cmet cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cmet
Entrez Gene ID	53561
Full Name	CENP-meta
Synonyms	CENP-E,CENP-E meta,CENP-META,CENP-Meta,CENPE,CENPmeta,CG6392,Cenp-E,Cenp-M,Cenp-meta,Cenp_E,Cmet,DmCmet,DmCmeta,Dmel\CG6392,KIF 10,KIF10,l(2)04431
General protein information	Preferred Names CENP-meta Names CG6392 gene product from transcript CG6392-RF CG6392-PC CG6392-PD CG6392-PE CG6392-PF Cenp-meta CenpMeta Kinesin2A cmet-PC cmet-PD cmet-PE cmet-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32E2-32E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_080254.3	NP_524993.3	CENP-meta, isoform C
NM_001273451.1	NP_001260380.1	CENP-meta, isoform D
NM_001273452.1	NP_001260381.1	CENP-meta, isoform E
NM_001273453.1	NP_001260382.1	CENP-meta, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	cmet	NP_524993.3
Drosophila melanogaster (fruit fly)	cana	NP_001027247.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

kinetochores movement depends on SPC105R, can occur in the absence of NDC80, and is antagonized by plus-end directed forces from the CENP-E motor.
Title: Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes.

Depletion of CENP-E or Nsl1-the latter mediating kinetochore targeting of Bub3-in epithelial tissues unable to activate the apoptotic program induces significant levels of aneuploidy and drives tumor-like growth.
Title: Tumor suppressor roles of CENP-E and Nsl1 in Drosophila epithelial tissues.

CENP-E appears to be required to destabilize kinetochore attachment. Activation of the mechanism that corrects inappropriate kinetochore attachment requires the antagonistic effects of BubR1 and CENP-E.
Title: BubR1 and CENP-E have antagonistic effects upon the stability of microtubule-kinetochore attachments in Drosophila S2 cell mitosis.

The following cmet gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cmet cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15342	NM_080254.3 Latest version!	Drosophila melanogaster CENP-meta (cmet), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15784	NM_001273453.1 Latest version!	Drosophila melanogaster CENP-meta (cmet), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15785	NM_001273451.1 Latest version!	Drosophila melanogaster CENP-meta (cmet), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15786	NM_001273452.1 Latest version!	Drosophila melanogaster CENP-meta (cmet), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cmet ( NM_080254.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524993.3 Drosophila melanogaster CENP-meta (cmet), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15342
Clone ID Related Accession (Same CDS sequence)	NM_080254.3
Accession Version	NM_080254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CENP-meta, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080254.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CAACGGATAA AATCAAGAAG GAAATCCAGG ACCTGCAAAT GTTCACCAGC 
TTAGAGAAGC ATTTTGAGGT GGAGTGCGAA GAGGTGCAGG GTTTAAAAGA AAAGCTGGCC 
GAGGTTACAG CACAGCGGGA TAATTTAGAG CAAGAATCGT TAGCCGAAAA GGAGCGTTAT 
GATGCTCTGG AAAAGGAAGT AACCAGCCTA AGAGCGGATA ATGAGGCTGC GAATTCAAAA 
ATCAGCGAAT TGGAAGAGAA ACTGAGTACT CTAAAGCAGA CTATGAGAAT AATGGAAGTG 
GAAAATCAGG TGGCCGTGGG CCTCGAGTTT GAGTTTGAGG CTCACAAGAA ATCATCGAAA 
CTTCGCGTGG ATGACTTACT CTCTGCCCTT TTAGAAAAGG AGTCGACTAT AGAGAGTCTT 
CAAAAGTCCC TCGACAATTT AACCCGCGAT GTCTTACGGA ACAGCAAGGA GGGCCACATG 
CTTTCCATTG CCCCAGAGCA GGAAGACATC GCCGGCGACA GTATATGCAA CAAGTGTGAG 
GAACTGGAGA AGCTGATTGC AGATTTAGAG TCCAAGAAAA ATTCATGCGA GTGTGATCAG 
CTCCGGTTGG AAATCGTCTC TGTTCGCGAC AAGTTAGAGA GCGTTGAATC TGCCTTTAAC 
CTGGCAAGCT CGGAGATTAT CCAAAAGGCG ACTGATTGCG AGCGACTTTC GAAAGAGCTA 
TCCACATCCC AGAATGCCTT TGGACAACTT CAGGAAAGGT ACGACGCTCT GGACCAACAG 
TGGCAAGCTC AACAAGCGGG CATCACAACC CTACATGAAA AGCACGAGCA CGTGCAGGAG 
AAATACCAGA AATTGCAGGA GGAGTATGAG CAGTTAGAAA GCAGGGCAAG AAGTGCCTCC 
AGCGCTGAGT TTCAACGGCT TCAAAATGAC AACACGAAGT TTCAAGCCGA TATCGCATCC 
CTCAATGAAC GATTGGAGGA GGCCCAAAAC ATGCTTACGG AAGTCCAAAA CTCTGAATCT 
ACTGTGGAAA AGCTTCGAAT ACAAAATCAC GAACTTACAG CGAAAATCAA AGAACTGGAG 
ACGAACTTTG AAGAGATGCA GCGGGAATAC GATTGCCTTT CCAATCAACT GATGGAGAGT 
GTCCAGGAGA ACGATGCCCT GCGAGAGGAG ATAAAGCAAC GACCCACGAG TCACGTTGAG 
GAGTCTATGA GGAGTTCCGG TATAAGCTCC GATTTTGACG AACAGAAGCA GGATATAAAC 
CTTCTACACC AGTTTGTTCA GCTTTCTGAG TCCGTCCAAC AGATAGAGCT GCAGCATCAT 
TCCGGCATCA GTCGCCTCTT TAGAGCCAAC CAAATGAAAC TGGATCAGAG CGAGCCAGGA 
CTTAAGCTTT GCCTTGAGTC TGCTGAGTAC ATCGAGGAAG ACAATCGTCA ATCCGATGCA 
ACTGAACCTA TTTGTCTCAA AGGCTTTCTC AAGCGACACA GATTTCAGAT AAAGCGACTT 
AGCCAGGAAC ATGTAGATAT GGGAGAGGAG AAGCGATTGC TTGATATTAT TTCGCAGCTG 
GAACAGGAAA TCGAAGAGAA GAGTGCACTT ATGGAGGCAA CAGAGGCAAC CATCAATGAA 
ATGCGAGAGC AGATGACTAA CCTAGAGTCG GCACTCCTCG AAAAGAGCGT TATAATTAAC 
AAGGTTGAGG ACTACCAGCG GCAGATCGAG TCCCTGGAAA AACAAAATGC GGAGATGACA 
ATGGTATACG AGGAGCTGCA GGATAGAGTA ACAAGGGAGA GCTCGATGAG CGAGAGTCTG 
CTCCGAGTTC CACCTGACGA GGATACCCTT CCAGGATGCC CTACATCTCC CAGTAGACGA 
GAACAGGAGG TGGCCACGCT AAAGACATCC ATAACAGAAT TGCAGTCACA AGTTTCTGAT 
CTAAAAGCAG AACTTGAAAA CCATTTAAGG CAGATCCAGT TGAAAGACGG GAACATAGCG 
AGACTACAGA CGGATTTTGA GGAAATGAGT GAGCGCTGCT TATCGATGGA GGTGAGGCTA 
GCCGAGTTGG ATGAGGACAC TAAGCAAAAA CAAGAACTAC TGGATCGTCA GGCGCAGAAG 
CTCTCCGATG ACCTATGCCT TATCGATCAG CTTCAAAAGA AAAACGCGCA GCTCGTTGAA 
CAGTATCATA AAGCGACAGA ATCTCTCAGT CTTGCGGATG CAAAGCCGGA TCAGATACTA 
CTTTCCTCCC AATATGATAG TCAGATCGAG AAACTTAATC AATTGCTAAA TGCAGCCAAA 
GATGAGCTAC ACGATGTGCG AAGGATAAAA GATGATGAGA TAAGTGCTTT GCGAATGGAG 
TTCCTGCTCC AAATTGAAAC GAATGAGAAA GAGAACCAAG CCAAGTTCTA CGCTGAATTG 
CAGGAGACTA AGGATCGCTA TGAGAGCAAT GTGGCCGAGT TAAAGGAGAA GCTATTGCAG 
GTAGAGGAGA CACTCTCCAG CGTTACGGTT CGTTGTCAGG CGGAGTTGGA AGCGCTTAAA 
TCTGCTCATA AAGAAAATAT TTCTCAGGCT GTGGAGGAAA GAAACAATTT GATTGTCCAG 
CACCAGGCAG AAATGGAGAC TATCAGGGAA ACTCTTAAGA ATAAGCTAGC TGAGGCTAGC 
ACCCAACAAT CAAAAATGGA AGATGCCTTT AGAGCGGAAA TAAACGAGGT GAGAGCCACC 
CTAATGGAGC AATTGAATCA AACGAAAGAA GATCGCGATA AGGGAGCCTC GAAGCTTGAG 
GAGGTCAAAA AGACACTGGA GCAGATGATA AATGGAGGAC GTGTCATGTC AGACACCATT 
GCTGAGTTGG AGAAAACAAA AGCTGAGCAG GACTTGGCAG TCAATAAATT AACAAAGGAC 
AACATCGAAT TAGAGAAGCA GTGCAGTAAG ACGCAGGAGC AGTTGCAAAT GGAATCGCTT 
ACTCGAGATC AAATCAGTTT CGAAATAGAA GCACATATTA AAAAGCTGGA ATTGATTGTG 
GCCTCTTCAA AGAAAAGGAT TATTGAGCTC GAGGAAAAGT GTGACCAGCA GGTTCTGGAA 
TTGGATAAAT GTAGGCTCGA GAAGTTGTCC CTGGAATCAG AGATTCAAAA AGCGAATTCA 
GAGCATTCGT GCACCATGGA AAAACTTCAG GAACTCCAAG CTGAAATGAA AGTTCTAAGC 
AACCGGAATG AGAAGGAAAA GTGTGATTTC GAAACTAAGC TCGAGACATT TACTTTCAAG 
ATAACCGATC TCGAAGAAGT CCTGAAGGAG GCACAACATA AAGTCATTCT CTATGACGAT 
TTGGTATCGC AGCACGAACG CCTAAAAATC TGTCTTGCCG AGGCAAATGA ACTATCATCA 
AACTTGCAAA AGAAAGTGAT GAGCCTGCAC ACGGAATTGA TTGATTCCCA AAAGGGAATA 
TCGAGTCGAG ATGTGGAAAT TAATGAGCTA CGCGAAGAAC TGAAGGCCGC TATGGATGCC 
AAGGCAACCG CGAGTGCCGA ACAAATGACT CTGGTAACGC AGCTCAAAGA TGTCGAGGAG 
CGGATGGCAA ACCAGGCAGA AAAGTTCACA CGTGAGGCGG CTAATCTAAA AGGGTCTATA 
AACGAGCTCC TCTTGAAGCT CAATTCCATG CAAGAAACTA AAGATATGCT AGAGTCCGGA 
AATGAAGAAC TCAAAGAACA GCTGAGAAAT TCCCAAAATC TGCGGAACAT GCTGGATGAG 
GAGAGTAAGA TGTGTATCTC CCTAAAAGAA AAATTAGTCA AACTGGAAGA TGCAAAAACT 
AGCCTTGAAC AGCAGTTGCG AGACAATAAA TCAGAAATAT ATCAAAGGCA CACGGAGCTT 
ACCAAGGAGG TGGAGCTGGG CCGCAACAGA ATTGGAGAAC TCACAAAGAA GTGTGAGGAG 
CTGTGCTCTG ATCTGGAAAA CTCAGACCAG ATACGACTCG ATCTGCAAGA AACTAAAGAG 
CAGTTGAAGA AAACGTTGGA AAATAACCTT GGATGGCAAC AGAAGGTTGA CGAAGTGACA 
AGAGAGTGCG AAAAGCTACG ATTTGATATG CAATCCAAAG AGGTGCAAAA TGAAAGTAAG 
GTTCAGGAAT TGATAAGCGA GTGCGAAGAG CTCCGGTCAA CTCTGAAATC CAAGGAAGCC 
AGTTTTCAGT CTGAGAAAGA AAGCATGGAC AGAACAATCT CAAGTCTTTT AGAAGACAAA 
CGCAACCTCG AGGAAAAGTT GTGCTCGGCT AACGATATTG TGGCTAAGCT TGAGACGGAA 
ATAGCCGCGT TGCGGCCAAG AAAAAGTCTA GATCGGAACC CAGTGCCGAG GAAATCGATT 
ACCTTTGAAT CCGAAATACG GAAGAATCGA CGAATCAGCG TCCACGATGA GCGACGGCAG 
TCTTACTGGA ACGACGTTCG GGAATTTGGC ATCATGACAG ATCCCGTTGA CAACAACTGC 
AACTGTGCGG AGCTGAATTC CAAGCTTCAG GACTGCCAGC GTGAGCTTTT CATTCGTGAA 
AGTCAAGTGA CGGCACTAAA GATGGAGCTG GACCATCATC CGCTGAAGGA TGAGAATGCG 
CAACTAACGA AGCGAGTTAT TGAGGAGCAA GACAAGGCAA AGGTTGAGCA GAAACGGCTT 
AAAATGAAAC TTCAAGATCT TAATGCTAGG ATTAATGACC TCACTACGGC ATCAGCCAAG 
GAACCAGAAT CTAACCAAAT GGCACAAGCA GCTAAACCTG CTACCGTAGC CGCTCAAACT 
CAGACGGAAT CCGATCTTGA GACAATCCTT GAGAAAACGA ACGTAAAGTA TCAGGAAGCG 
GTTCGCATGT TGCGCTACCG TTACCATCTC ATCCAGGAAC TGAAAGAGAA ACTTAGGCAA 
AACGAAAACA GTGATACCTC GAATATTACC TCACTCTCAG CTGGACAAAC CAGTGCATTG 
AAGGCACAGT GTGAGTCCCA AAAGAAGGAA ATTTTGGCAA TCAAATATAA GTATGAAGCC 
GCCAAGCGAA TTTTGGCGAT TCGAAATGAT GATTTGGATG CTCTGCGGGA AAAACTCGCG 
AAATATGAAA CAACTACATC AGATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524993.3
CDS	157..6726
Translation	MSAKNASSIQVCIKVRPCEPGLTSLWQVKERRSIHLADSHAEPYVFDYVFDEGASNQEVFDRMAKHIVHACMQGFNGTIFAYGQTSSGKTYTMMGDEQNPGVMVLAAKEIFQQISSETERDFLLRVGYIEIYNEKIYDLLNKKNQDLKIHESGNGIVNVNCEECIITSEVDLLRLLCLGNKERTVGETNMNERSSRSHAIFKIIIESRKSDHSDDDAVIQSVLNLVDLAGSERADQTGARGARLKEGGHINKSLLFLSNVIKSLSENADNRFTNYRDSKLTRILQASLGGNAFTSIICTIKPSIMEESQSTLSFATRAKKIRIKPQVNEMVSDATMMKRLEREIKVLKDKLAEEERKNENQQKVEHLERQIKHDMHKIICGHSLSDKGQQKRRRTWCPTASGSHLELAETGTTEDRIDQFPKVSHLPKPVFFHTSNAGKRWDNIPKTINILGSLDIGTESNSISEEFLPAECIDFGSPRPDVLKPMLTIRQLPDLPLTPKGPLTTDKIKKEIQDLQMFTSLEKHFEVECEEVQGLKEKLAEVTAQRDNLEQESLAEKERYDALEKEVTSLRADNEAANSKISELEEKLSTLKQTMRIMEVENQVAVGLEFEFEAHKKSSKLRVDDLLSALLEKESTIESLQKSLDNLTRDVLRNSKEGHMLSIAPEQEDIAGDSICNKCEELEKLIADLESKKNSCECDQLRLEIVSVRDKLESVESAFNLASSEIIQKATDCERLSKELSTSQNAFGQLQERYDALDQQWQAQQAGITTLHEKHEHVQEKYQKLQEEYEQLESRARSASSAEFQRLQNDNTKFQADIASLNERLEEAQNMLTEVQNSESTVEKLRIQNHELTAKIKELETNFEEMQREYDCLSNQLMESVQENDALREEIKQRPTSHVEESMRSSGISSDFDEQKQDINLLHQFVQLSESVQQIELQHHSGISRLFRANQMKLDQSEPGLKLCLESAEYIEEDNRQSDATEPICLKGFLKRHRFQIKRLSQEHVDMGEEKRLLDIISQLEQEIEEKSALMEATEATINEMREQMTNLESALLEKSVIINKVEDYQRQIESLEKQNAEMTMVYEELQDRVTRESSMSESLLRVPPDEDTLPGCPTSPSRREQEVATLKTSITELQSQVSDLKAELENHLRQIQLKDGNIARLQTDFEEMSERCLSMEVRLAELDEDTKQKQELLDRQAQKLSDDLCLIDQLQKKNAQLVEQYHKATESLSLADAKPDQILLSSQYDSQIEKLNQLLNAAKDELHDVRRIKDDEISALRMEFLLQIETNEKENQAKFYAELQETKDRYESNVAELKEKLLQVEETLSSVTVRCQAELEALKSAHKENISQAVEERNNLIVQHQAEMETIRETLKNKLAEASTQQSKMEDAFRAEINEVRATLMEQLNQTKEDRDKGASKLEEVKKTLEQMINGGRVMSDTIAELEKTKAEQDLAVNKLTKDNIELEKQCSKTQEQLQMESLTRDQISFEIEAHIKKLELIVASSKKRIIELEEKCDQQVLELDKCRLEKLSLESEIQKANSEHSCTMEKLQELQAEMKVLSNRNEKEKCDFETKLETFTFKITDLEEVLKEAQHKVILYDDLVSQHERLKICLAEANELSSNLQKKVMSLHTELIDSQKGISSRDVEINELREELKAAMDAKATASAEQMTLVTQLKDVEERMANQAEKFTREAANLKGSINELLLKLNSMQETKDMLESGNEELKEQLRNSQNLRNMLDEESKMCISLKEKLVKLEDAKTSLEQQLRDNKSEIYQRHTELTKEVELGRNRIGELTKKCEELCSDLENSDQIRLDLQETKEQLKKTLENNLGWQQKVDEVTRECEKLRFDMQSKEVQNESKVQELISECEELRSTLKSKEASFQSEKESMDRTISSLLEDKRNLEEKLCSANDIVAKLETEIAALRPRKSLDRNPVPRKSITFESEIRKNRRISVHDERRQSYWNDVREFGIMTDPVDNNCNCAELNSKLQDCQRELFIRESQVTALKMELDHHPLKDENAQLTKRVIEEQDKAKVEQKRLKMKLQDLNARINDLTTASAKEPESNQMAQAAKPATVAAQTQTESDLETILEKTNVKYQEAVRMLRYRYHLIQELKEKLRQNENSDTSNITSLSAGQTSALKAQCESQKKEILAIKYKYEAAKRILAIRNDDLDALREKLAKYETTTSDK

Target ORF information:

RefSeq Version	NM_080254.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CENP-meta (cmet), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080254.3

ORF Insert Sequence:

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CAACGGATAA AATCAAGAAG GAAATCCAGG ACCTGCAAAT GTTCACCAGC 
TTAGAGAAGC ATTTTGAGGT GGAGTGCGAA GAGGTGCAGG GTTTAAAAGA AAAGCTGGCC 
GAGGTTACAG CACAGCGGGA TAATTTAGAG CAAGAATCGT TAGCCGAAAA GGAGCGTTAT 
GATGCTCTGG AAAAGGAAGT AACCAGCCTA AGAGCGGATA ATGAGGCTGC GAATTCAAAA 
ATCAGCGAAT TGGAAGAGAA ACTGAGTACT CTAAAGCAGA CTATGAGAAT AATGGAAGTG 
GAAAATCAGG TGGCCGTGGG CCTCGAGTTT GAGTTTGAGG CTCACAAGAA ATCATCGAAA 
CTTCGCGTGG ATGACTTACT CTCTGCCCTT TTAGAAAAGG AGTCGACTAT AGAGAGTCTT 
CAAAAGTCCC TCGACAATTT AACCCGCGAT GTCTTACGGA ACAGCAAGGA GGGCCACATG 
CTTTCCATTG CCCCAGAGCA GGAAGACATC GCCGGCGACA GTATATGCAA CAAGTGTGAG 
GAACTGGAGA AGCTGATTGC AGATTTAGAG TCCAAGAAAA ATTCATGCGA GTGTGATCAG 
CTCCGGTTGG AAATCGTCTC TGTTCGCGAC AAGTTAGAGA GCGTTGAATC TGCCTTTAAC 
CTGGCAAGCT CGGAGATTAT CCAAAAGGCG ACTGATTGCG AGCGACTTTC GAAAGAGCTA 
TCCACATCCC AGAATGCCTT TGGACAACTT CAGGAAAGGT ACGACGCTCT GGACCAACAG 
TGGCAAGCTC AACAAGCGGG CATCACAACC CTACATGAAA AGCACGAGCA CGTGCAGGAG 
AAATACCAGA AATTGCAGGA GGAGTATGAG CAGTTAGAAA GCAGGGCAAG AAGTGCCTCC 
AGCGCTGAGT TTCAACGGCT TCAAAATGAC AACACGAAGT TTCAAGCCGA TATCGCATCC 
CTCAATGAAC GATTGGAGGA GGCCCAAAAC ATGCTTACGG AAGTCCAAAA CTCTGAATCT 
ACTGTGGAAA AGCTTCGAAT ACAAAATCAC GAACTTACAG CGAAAATCAA AGAACTGGAG 
ACGAACTTTG AAGAGATGCA GCGGGAATAC GATTGCCTTT CCAATCAACT GATGGAGAGT 
GTCCAGGAGA ACGATGCCCT GCGAGAGGAG ATAAAGCAAC GACCCACGAG TCACGTTGAG 
GAGTCTATGA GGAGTTCCGG TATAAGCTCC GATTTTGACG AACAGAAGCA GGATATAAAC 
CTTCTACACC AGTTTGTTCA GCTTTCTGAG TCCGTCCAAC AGATAGAGCT GCAGCATCAT 
TCCGGCATCA GTCGCCTCTT TAGAGCCAAC CAAATGAAAC TGGATCAGAG CGAGCCAGGA 
CTTAAGCTTT GCCTTGAGTC TGCTGAGTAC ATCGAGGAAG ACAATCGTCA ATCCGATGCA 
ACTGAACCTA TTTGTCTCAA AGGCTTTCTC AAGCGACACA GATTTCAGAT AAAGCGACTT 
AGCCAGGAAC ATGTAGATAT GGGAGAGGAG AAGCGATTGC TTGATATTAT TTCGCAGCTG 
GAACAGGAAA TCGAAGAGAA GAGTGCACTT ATGGAGGCAA CAGAGGCAAC CATCAATGAA 
ATGCGAGAGC AGATGACTAA CCTAGAGTCG GCACTCCTCG AAAAGAGCGT TATAATTAAC 
AAGGTTGAGG ACTACCAGCG GCAGATCGAG TCCCTGGAAA AACAAAATGC GGAGATGACA 
ATGGTATACG AGGAGCTGCA GGATAGAGTA ACAAGGGAGA GCTCGATGAG CGAGAGTCTG 
CTCCGAGTTC CACCTGACGA GGATACCCTT CCAGGATGCC CTACATCTCC CAGTAGACGA 
GAACAGGAGG TGGCCACGCT AAAGACATCC ATAACAGAAT TGCAGTCACA AGTTTCTGAT 
CTAAAAGCAG AACTTGAAAA CCATTTAAGG CAGATCCAGT TGAAAGACGG GAACATAGCG 
AGACTACAGA CGGATTTTGA GGAAATGAGT GAGCGCTGCT TATCGATGGA GGTGAGGCTA 
GCCGAGTTGG ATGAGGACAC TAAGCAAAAA CAAGAACTAC TGGATCGTCA GGCGCAGAAG 
CTCTCCGATG ACCTATGCCT TATCGATCAG CTTCAAAAGA AAAACGCGCA GCTCGTTGAA 
CAGTATCATA AAGCGACAGA ATCTCTCAGT CTTGCGGATG CAAAGCCGGA TCAGATACTA 
CTTTCCTCCC AATATGATAG TCAGATCGAG AAACTTAATC AATTGCTAAA TGCAGCCAAA 
GATGAGCTAC ACGATGTGCG AAGGATAAAA GATGATGAGA TAAGTGCTTT GCGAATGGAG 
TTCCTGCTCC AAATTGAAAC GAATGAGAAA GAGAACCAAG CCAAGTTCTA CGCTGAATTG 
CAGGAGACTA AGGATCGCTA TGAGAGCAAT GTGGCCGAGT TAAAGGAGAA GCTATTGCAG 
GTAGAGGAGA CACTCTCCAG CGTTACGGTT CGTTGTCAGG CGGAGTTGGA AGCGCTTAAA 
TCTGCTCATA AAGAAAATAT TTCTCAGGCT GTGGAGGAAA GAAACAATTT GATTGTCCAG 
CACCAGGCAG AAATGGAGAC TATCAGGGAA ACTCTTAAGA ATAAGCTAGC TGAGGCTAGC 
ACCCAACAAT CAAAAATGGA AGATGCCTTT AGAGCGGAAA TAAACGAGGT GAGAGCCACC 
CTAATGGAGC AATTGAATCA AACGAAAGAA GATCGCGATA AGGGAGCCTC GAAGCTTGAG 
GAGGTCAAAA AGACACTGGA GCAGATGATA AATGGAGGAC GTGTCATGTC AGACACCATT 
GCTGAGTTGG AGAAAACAAA AGCTGAGCAG GACTTGGCAG TCAATAAATT AACAAAGGAC 
AACATCGAAT TAGAGAAGCA GTGCAGTAAG ACGCAGGAGC AGTTGCAAAT GGAATCGCTT 
ACTCGAGATC AAATCAGTTT CGAAATAGAA GCACATATTA AAAAGCTGGA ATTGATTGTG 
GCCTCTTCAA AGAAAAGGAT TATTGAGCTC GAGGAAAAGT GTGACCAGCA GGTTCTGGAA 
TTGGATAAAT GTAGGCTCGA GAAGTTGTCC CTGGAATCAG AGATTCAAAA AGCGAATTCA 
GAGCATTCGT GCACCATGGA AAAACTTCAG GAACTCCAAG CTGAAATGAA AGTTCTAAGC 
AACCGGAATG AGAAGGAAAA GTGTGATTTC GAAACTAAGC TCGAGACATT TACTTTCAAG 
ATAACCGATC TCGAAGAAGT CCTGAAGGAG GCACAACATA AAGTCATTCT CTATGACGAT 
TTGGTATCGC AGCACGAACG CCTAAAAATC TGTCTTGCCG AGGCAAATGA ACTATCATCA 
AACTTGCAAA AGAAAGTGAT GAGCCTGCAC ACGGAATTGA TTGATTCCCA AAAGGGAATA 
TCGAGTCGAG ATGTGGAAAT TAATGAGCTA CGCGAAGAAC TGAAGGCCGC TATGGATGCC 
AAGGCAACCG CGAGTGCCGA ACAAATGACT CTGGTAACGC AGCTCAAAGA TGTCGAGGAG 
CGGATGGCAA ACCAGGCAGA AAAGTTCACA CGTGAGGCGG CTAATCTAAA AGGGTCTATA 
AACGAGCTCC TCTTGAAGCT CAATTCCATG CAAGAAACTA AAGATATGCT AGAGTCCGGA 
AATGAAGAAC TCAAAGAACA GCTGAGAAAT TCCCAAAATC TGCGGAACAT GCTGGATGAG 
GAGAGTAAGA TGTGTATCTC CCTAAAAGAA AAATTAGTCA AACTGGAAGA TGCAAAAACT 
AGCCTTGAAC AGCAGTTGCG AGACAATAAA TCAGAAATAT ATCAAAGGCA CACGGAGCTT 
ACCAAGGAGG TGGAGCTGGG CCGCAACAGA ATTGGAGAAC TCACAAAGAA GTGTGAGGAG 
CTGTGCTCTG ATCTGGAAAA CTCAGACCAG ATACGACTCG ATCTGCAAGA AACTAAAGAG 
CAGTTGAAGA AAACGTTGGA AAATAACCTT GGATGGCAAC AGAAGGTTGA CGAAGTGACA 
AGAGAGTGCG AAAAGCTACG ATTTGATATG CAATCCAAAG AGGTGCAAAA TGAAAGTAAG 
GTTCAGGAAT TGATAAGCGA GTGCGAAGAG CTCCGGTCAA CTCTGAAATC CAAGGAAGCC 
AGTTTTCAGT CTGAGAAAGA AAGCATGGAC AGAACAATCT CAAGTCTTTT AGAAGACAAA 
CGCAACCTCG AGGAAAAGTT GTGCTCGGCT AACGATATTG TGGCTAAGCT TGAGACGGAA 
ATAGCCGCGT TGCGGCCAAG AAAAAGTCTA GATCGGAACC CAGTGCCGAG GAAATCGATT 
ACCTTTGAAT CCGAAATACG GAAGAATCGA CGAATCAGCG TCCACGATGA GCGACGGCAG 
TCTTACTGGA ACGACGTTCG GGAATTTGGC ATCATGACAG ATCCCGTTGA CAACAACTGC 
AACTGTGCGG AGCTGAATTC CAAGCTTCAG GACTGCCAGC GTGAGCTTTT CATTCGTGAA 
AGTCAAGTGA CGGCACTAAA GATGGAGCTG GACCATCATC CGCTGAAGGA TGAGAATGCG 
CAACTAACGA AGCGAGTTAT TGAGGAGCAA GACAAGGCAA AGGTTGAGCA GAAACGGCTT 
AAAATGAAAC TTCAAGATCT TAATGCTAGG ATTAATGACC TCACTACGGC ATCAGCCAAG 
GAACCAGAAT CTAACCAAAT GGCACAAGCA GCTAAACCTG CTACCGTAGC CGCTCAAACT 
CAGACGGAAT CCGATCTTGA GACAATCCTT GAGAAAACGA ACGTAAAGTA TCAGGAAGCG 
GTTCGCATGT TGCGCTACCG TTACCATCTC ATCCAGGAAC TGAAAGAGAA ACTTAGGCAA 
AACGAAAACA GTGATACCTC GAATATTACC TCACTCTCAG CTGGACAAAC CAGTGCATTG 
AAGGCACAGT GTGAGTCCCA AAAGAAGGAA ATTTTGGCAA TCAAATATAA GTATGAAGCC 
GCCAAGCGAA TTTTGGCGAT TCGAAATGAT GATTTGGATG CTCTGCGGGA AAAACTCGCG 
AAATATGAAA CAACTACATC AGATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cmet ( NM_001273453.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260382.1 Drosophila melanogaster CENP-meta (cmet), transcript variant F, mRNA.

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CloneID	OFa15784
Clone ID Related Accession (Same CDS sequence)	NM_001273453.1
Accession Version	NM_001273453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CENP-meta, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CTTATGATGC TCTGGAAAAG GAAGTAACCA GCCTAAGAGC GGATAATGAG 
GCTGCGAATT CAAAAATCAG CGAATTGGAA GAGAAACTGA GTACTCTAAA GCAGACTATG 
AGAATAATGG AAGTGGAAAA TCAGGTGGCC GTGGGCCTCG AGTTTGAGTT TGAGGCTCAC 
AAGAAATCAT CGAAACTTCG CGTGGATGAC TTACTCTCTG CCCTTTTAGA AAAGGAGTCG 
ACTATAGAGA GTCTTCAAAA GTCCCTCGAC AATTTAACCC GCGATGTCTT ACGGAACAGC 
AAGGAGGGCC ACATGCTTTC CATTGCCCCA GAGCAGGAAG ACATCGCCGG CGACAGTATA 
TGCAACAAGT GTGAGGAACT GGAGAAGCTG ATTGCAGATT TAGAGTCCAA GAAAAATTCA 
TGCGAGTGTG ATCAGCTCCG GTTGGAAATC GTCTCTGTTC GCGACAAGTT AGAGAGCGTT 
GAATCTGCCT TTAACCTGGC AAGCTCGGAG ATTATCCAAA AGGCGACTGA TTGCGAGCGA 
CTTTCGAAAG AGCTATCCAC ATCCCAGAAT GCCTTTGGAC AACTTCAGGA AAGGTACGAC 
GCTCTGGACC AACAGTGGCA AGCTCAACAA GCGGGCATCA CAACCCTACA TGAAAAGCAC 
GAGCACGTGC AGGAGAAATA CCAGAAATTG CAGGAGGAGT ATGAGCAGTT AGAAAGCAGG 
GCAAGAAGTG CCTCCAGCGC TGAGTTTCAA CGGCTTCAAA ATGACAACAC GAAGTTTCAA 
GCCGATATCG CATCCCTCAA TGAACGATTG GAGGAGGCCC AAAACATGCT TACGGAAGTC 
CAAAACTCTG AATCTACTGT GGAAAAGCTT CGAATACAAA ATCACGAACT TACAGCGAAA 
ATCAAAGAAC TGGAGACGAA CTTTGAAGAG ATGCAGCGGG AATACGATTG CCTTTCCAAT 
CAACTGATGG AGAGTGTCCA GGAGAACGAT GCCCTGCGAG AGGAGATAAA GCAACGACCC 
ACGAGTCACG TTGAGGAGTC TATGAGGAGT TCCGGTATAA GCTCCGATTT TGACGAACAG 
AAGCAGGATA TAAACCTTCT ACACCAGTTT GTTCAGCTTT CTGAGTCCGT CCAACAGATA 
GAGCTGCAGC ATCATTCCGG CATCAGTCGC CTCTTTAGAG CCAACCAAAT GAAACTGGAT 
CAGAGCGAGC CAGGACTTAA GCTTTGCCTT GAGTCTGCTG AGTACATCGA GGAAGACAAT 
CGTCAATCCG ATGCAACTGA ACCTATTTGT CTCAAAGGCT TTCTCAAGCG ACACAGATTT 
CAGATAAAGC GACTTAGCCA GGAACATGTA GATATGGGAG AGGAGAAGCG ATTGCTTGAT 
ATTATTTCGC AGCTGGAACA GGAAATCGAA GAGAAGAGTG CACTTATGGA GGCAACAGAG 
GCAACCATCA ATGAAATGCG AGAGCAGATG ACTAACCTAG AGTCGGCACT CCTCGAAAAG 
AGCGTTATAA TTAACAAGGT TGAGGACTAC CAGCGGCAGA TCGAGTCCCT GGAAAAACAA 
AATGCGGAGA TGACAATGGT ATACGAGGAG CTGCAGGATA GAGTAACAAG GGAGAGCTCG 
ATGAGCGAGA GTCTGCTCCG AGTTCCACCT GACGAGGATA CCCTTCCAGG ATGCCCTACA 
TCTCCCAGTA GACGAGAACA GGAGGTGGCC ACGCTAAAGA CATCCATAAC AGAATTGCAG 
TCACAAGTTT CTGATCTAAA AGCAGAACTT GAAAACCATT TAAGGCAGAT CCAGTTGAAA 
GACGGGAACA TAGCGAGACT ACAGACGGAT TTTGAGGAAA TGAGTGAGCG CTGCTTATCG 
ATGGAGGTGA GGCTAGCCGA GTTGGATGAG GACACTAAGC AAAAACAAGA ACTACTGGAT 
CGTCAGGCGC AGAAGCTCTC CGATGACCTA TGCCTTATCG ATCAGCTTCA AAAGAAAAAC 
GCGCAGCTCG TTGAACAGTA TCATAAAGCG ACAGAATCTC TCAGTCTTGC GGATGCAAAG 
CCGGATCAGA TACTACTTTC CTCCCAATAT GATAGTCAGA TCGAGAAACT TAATCAATTG 
CTAAATGCAG CCAAAGATGA GCTACACGAT GTGCGAAGGA TAAAAGATGA TGAGATAAGT 
GCTTTGCGAA TGGAGTTCCT GCTCCAAATT GAAACGAATG AGAAAGAGAA CCAAGCCAAG 
TTCTACGCTG AATTGCAGGA GACTAAGGAT CGCTATGAGA GCAATGTGGC CGAGTTAAAG 
GAGAAGCTAT TGCAGGTAGA GGAGACACTC TCCAGCGTTA CGGTTCGTTG TCAGGCGGAG 
TTGGAAGCGC TTAAATCTGC TCATAAAGAA AATATTTCTC AGGCTGTGGA GGAAAGAAAC 
AATTTGATTG TCCAGCACCA GGCAGAAATG GAGACTATCA GGGAAACTCT TAAGAATAAG 
CTAGCTGAGG CTAGCACCCA ACAATCAAAA ATGGAAGATG CCTTTAGAGC GGAAATAAAC 
GAGGTGAGAG CCACCCTAAT GGAGCAATTG AATCAAACGA AAGAAGATCG CGATAAGGGA 
GCCTCGAAGC TTGAGGAGGT CAAAAAGACA CTGGAGCAGA TGATAAATGG AGGACGTGTC 
ATGTCAGACA CCATTGCTGA GTTGGAGAAA ACAAAAGCTG AGCAGGACTT GGCAGTCAAT 
AAATTAACAA AGGACAACAT CGAATTAGAG AAGCAGTGCA GTAAGACGCA GGAGCAGTTG 
CAAATGGAAT CGCTTACTCG AGATCAAATC AGTTTCGAAA TAGAAGCACA TATTAAAAAG 
CTGGAATTGA TTGTGGCCTC TTCAAAGAAA AGGATTATTG AGCTCGAGGA AAAGTGTGAC 
CAGCAGGTTC TGGAATTGGA TAAATGTAGG CTCGAGAAGT TGTCCCTGGA ATCAGAGATT 
CAAAAAGCGA ATTCAGAGCA TTCGTGCACC ATGGAAAAAC TTCAGGAACT CCAAGCTGAA 
ATGAAAGTTC TAAGCAACCG GAATGAGAAG GAAAAGTGTG ATTTCGAAAC TAAGCTCGAG 
ACATTTACTT TCAAGATAAC CGATCTCGAA GAAGTCCTGA AGGAGGCACA ACATAAAGTC 
ATTCTCTATG ACGATTTGGT ATCGCAGCAC GAACGCCTAA AAATCTGTCT TGCCGAGGCA 
AATGAACTAT CATCAAACTT GCAAAAGAAA GTGATGAGCC TGCACACGGA ATTGATTGAT 
TCCCAAAAGG GAATATCGAG TCGAGATGTG GAAATTAATG AGCTACGCGA AGAACTGAAG 
GCCGCTATGG ATGCCAAGGC AACCGCGAGT GCCGAACAAA TGACTCTGGT AACGCAGCTC 
AAAGATGTCG AGGAGCGGAT GGCAAACCAG GCAGAAAAGT TCACACGTGA GGCGGCTAAT 
CTAAAAGGGT CTATAAACGA GCTCCTCTTG AAGCTCAATT CCATGCAAGA AACTAAAGAT 
ATGCTAGAGT CCGGAAATGA AGAACTCAAA GAACAGCTGA GAAATTCCCA AAATCTGCGG 
AACATGCTGG ATGAGGAGAG TAAGATGTGT ATCTCCCTAA AAGAAAAATT AGTCAAACTG 
GAAGATGCAA AAACTAGCCT TGAACAGCAG TTGCGAGACA ATAAATCAGA AATATATCAA 
AGGCACACGG AGCTTACCAA GGAGGTGGAG CTGGGCCGCA ACAGAATTGG AGAACTCACA 
AAGAAGTGTG AGGAGCTGTG CTCTGATCTG GAAAACTCAG ACCAGATACG ACTCGATCTG 
CAAGAAACTA AAGAGCAGTT GAAGAAAACG TTGGAAAATA ACCTTGGATG GCAACAGAAG 
GTTGACGAAG TGACAAGAGA GTGCGAAAAG CTACGATTTG ATATGCAATC CAAAGAGGTG 
CAAAATGAAA GTAAGGTTCA GGAATTGATA AGCGAGTGCG AAGAGCTCCG GTCAACTCTG 
AAATCCAAGG AAGCCAGTTT TCAGTCTGAG AAAGAAAGCA TGGACAGAAC AATCTCAAGT 
CTTTTAGAAG ACAAACGCAA CCTCGAGGAA AAGTTGTGCT CGGCTAACGA TATTGTGGCT 
AAGCTTGAGA CGGAAATAGC CGCGTTGCGG CCAAGAAAAA GTCTAGATCG GAACCCAGTG 
CCGAGGAAAT CGATTACCTT TGAATCCGAA ATACGGAAGA ATCGACGAAT CAGCGTCCAC 
GATGAGCGAC GGCAGTCTTA CTGGAACGAC GTTCGGGAAT TTGGCATCAT GACAGATCCC 
GTTGACAACA ACTGCAACTG TGCGGAGCTG AATTCCAAGC TTCAGGACTG CCAGCGTGAG 
CTTTTCATTC GTGAAAGTCA AGTGACGGCA CTAAAGATGG AGCTGGACCA TCATCCGCTG 
AAGGATGAGA ATGCGCAACT AACGAAGCGA GTTATTGAGG AGCAAGACAA GGCAAAGGTT 
GAGCAGAAAC GGCTTAAAAT GAAACTTCAA GATCTTAATG CTAGGATTAA TGACCTCACT 
ACGGCATCAG CCAAGGAACC AGAATCTAAC CAAATGGCAC AAGCAGCTAA ACCTGCTACC 
GTAGCCGCTC AAACTCAGAC GGAATCCGAT CTTGAGACAA TCCTTGAGAA AACGAACGTA 
AAGTATCAGG AAGCGGTTCG CATGTTGCGC TACCGTTACC ATCTCATCCA GGAACTGAAA 
GAGAAACTTA GGCAAAACGA AAACAGTGAT ACCTCGAATA TTACCTCACT CTCAGCTGGA 
CAAACCAGTG CATTGAAGTG TGAGTCCCAA AAGAAGGAAA TTTTGGCAAT CAAATATAAG 
TATGAAGCCG CCAAGCGAAT TTTGGCGATT CGAAATGATG ATTTGGATGC TCTGCGGGAA 
AAACTCGCGA AATATGAAAC AACTACATCA GATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260382.1
CDS	157..6555
Translation	MSAKNASSIQVCIKVRPCEPGLTSLWQVKERRSIHLADSHAEPYVFDYVFDEGASNQEVFDRMAKHIVHACMQGFNGTIFAYGQTSSGKTYTMMGDEQNPGVMVLAAKEIFQQISSETERDFLLRVGYIEIYNEKIYDLLNKKNQDLKIHESGNGIVNVNCEECIITSEVDLLRLLCLGNKERTVGETNMNERSSRSHAIFKIIIESRKSDHSDDDAVIQSVLNLVDLAGSERADQTGARGARLKEGGHINKSLLFLSNVIKSLSENADNRFTNYRDSKLTRILQASLGGNAFTSIICTIKPSIMEESQSTLSFATRAKKIRIKPQVNEMVSDATMMKRLEREIKVLKDKLAEEERKNENQQKVEHLERQIKHDMHKIICGHSLSDKGQQKRRRTWCPTASGSHLELAETGTTEDRIDQFPKVSHLPKPVFFHTSNAGKRWDNIPKTINILGSLDIGTESNSISEEFLPAECIDFGSPRPDVLKPMLTIRQLPDLPLTPKGPLTYDALEKEVTSLRADNEAANSKISELEEKLSTLKQTMRIMEVENQVAVGLEFEFEAHKKSSKLRVDDLLSALLEKESTIESLQKSLDNLTRDVLRNSKEGHMLSIAPEQEDIAGDSICNKCEELEKLIADLESKKNSCECDQLRLEIVSVRDKLESVESAFNLASSEIIQKATDCERLSKELSTSQNAFGQLQERYDALDQQWQAQQAGITTLHEKHEHVQEKYQKLQEEYEQLESRARSASSAEFQRLQNDNTKFQADIASLNERLEEAQNMLTEVQNSESTVEKLRIQNHELTAKIKELETNFEEMQREYDCLSNQLMESVQENDALREEIKQRPTSHVEESMRSSGISSDFDEQKQDINLLHQFVQLSESVQQIELQHHSGISRLFRANQMKLDQSEPGLKLCLESAEYIEEDNRQSDATEPICLKGFLKRHRFQIKRLSQEHVDMGEEKRLLDIISQLEQEIEEKSALMEATEATINEMREQMTNLESALLEKSVIINKVEDYQRQIESLEKQNAEMTMVYEELQDRVTRESSMSESLLRVPPDEDTLPGCPTSPSRREQEVATLKTSITELQSQVSDLKAELENHLRQIQLKDGNIARLQTDFEEMSERCLSMEVRLAELDEDTKQKQELLDRQAQKLSDDLCLIDQLQKKNAQLVEQYHKATESLSLADAKPDQILLSSQYDSQIEKLNQLLNAAKDELHDVRRIKDDEISALRMEFLLQIETNEKENQAKFYAELQETKDRYESNVAELKEKLLQVEETLSSVTVRCQAELEALKSAHKENISQAVEERNNLIVQHQAEMETIRETLKNKLAEASTQQSKMEDAFRAEINEVRATLMEQLNQTKEDRDKGASKLEEVKKTLEQMINGGRVMSDTIAELEKTKAEQDLAVNKLTKDNIELEKQCSKTQEQLQMESLTRDQISFEIEAHIKKLELIVASSKKRIIELEEKCDQQVLELDKCRLEKLSLESEIQKANSEHSCTMEKLQELQAEMKVLSNRNEKEKCDFETKLETFTFKITDLEEVLKEAQHKVILYDDLVSQHERLKICLAEANELSSNLQKKVMSLHTELIDSQKGISSRDVEINELREELKAAMDAKATASAEQMTLVTQLKDVEERMANQAEKFTREAANLKGSINELLLKLNSMQETKDMLESGNEELKEQLRNSQNLRNMLDEESKMCISLKEKLVKLEDAKTSLEQQLRDNKSEIYQRHTELTKEVELGRNRIGELTKKCEELCSDLENSDQIRLDLQETKEQLKKTLENNLGWQQKVDEVTRECEKLRFDMQSKEVQNESKVQELISECEELRSTLKSKEASFQSEKESMDRTISSLLEDKRNLEEKLCSANDIVAKLETEIAALRPRKSLDRNPVPRKSITFESEIRKNRRISVHDERRQSYWNDVREFGIMTDPVDNNCNCAELNSKLQDCQRELFIRESQVTALKMELDHHPLKDENAQLTKRVIEEQDKAKVEQKRLKMKLQDLNARINDLTTASAKEPESNQMAQAAKPATVAAQTQTESDLETILEKTNVKYQEAVRMLRYRYHLIQELKEKLRQNENSDTSNITSLSAGQTSALKCESQKKEILAIKYKYEAAKRILAIRNDDLDALREKLAKYETTTSDK

Target ORF information:

RefSeq Version	NM_001273453.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CENP-meta (cmet), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273453.1

ORF Insert Sequence:

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CTTATGATGC TCTGGAAAAG GAAGTAACCA GCCTAAGAGC GGATAATGAG 
GCTGCGAATT CAAAAATCAG CGAATTGGAA GAGAAACTGA GTACTCTAAA GCAGACTATG 
AGAATAATGG AAGTGGAAAA TCAGGTGGCC GTGGGCCTCG AGTTTGAGTT TGAGGCTCAC 
AAGAAATCAT CGAAACTTCG CGTGGATGAC TTACTCTCTG CCCTTTTAGA AAAGGAGTCG 
ACTATAGAGA GTCTTCAAAA GTCCCTCGAC AATTTAACCC GCGATGTCTT ACGGAACAGC 
AAGGAGGGCC ACATGCTTTC CATTGCCCCA GAGCAGGAAG ACATCGCCGG CGACAGTATA 
TGCAACAAGT GTGAGGAACT GGAGAAGCTG ATTGCAGATT TAGAGTCCAA GAAAAATTCA 
TGCGAGTGTG ATCAGCTCCG GTTGGAAATC GTCTCTGTTC GCGACAAGTT AGAGAGCGTT 
GAATCTGCCT TTAACCTGGC AAGCTCGGAG ATTATCCAAA AGGCGACTGA TTGCGAGCGA 
CTTTCGAAAG AGCTATCCAC ATCCCAGAAT GCCTTTGGAC AACTTCAGGA AAGGTACGAC 
GCTCTGGACC AACAGTGGCA AGCTCAACAA GCGGGCATCA CAACCCTACA TGAAAAGCAC 
GAGCACGTGC AGGAGAAATA CCAGAAATTG CAGGAGGAGT ATGAGCAGTT AGAAAGCAGG 
GCAAGAAGTG CCTCCAGCGC TGAGTTTCAA CGGCTTCAAA ATGACAACAC GAAGTTTCAA 
GCCGATATCG CATCCCTCAA TGAACGATTG GAGGAGGCCC AAAACATGCT TACGGAAGTC 
CAAAACTCTG AATCTACTGT GGAAAAGCTT CGAATACAAA ATCACGAACT TACAGCGAAA 
ATCAAAGAAC TGGAGACGAA CTTTGAAGAG ATGCAGCGGG AATACGATTG CCTTTCCAAT 
CAACTGATGG AGAGTGTCCA GGAGAACGAT GCCCTGCGAG AGGAGATAAA GCAACGACCC 
ACGAGTCACG TTGAGGAGTC TATGAGGAGT TCCGGTATAA GCTCCGATTT TGACGAACAG 
AAGCAGGATA TAAACCTTCT ACACCAGTTT GTTCAGCTTT CTGAGTCCGT CCAACAGATA 
GAGCTGCAGC ATCATTCCGG CATCAGTCGC CTCTTTAGAG CCAACCAAAT GAAACTGGAT 
CAGAGCGAGC CAGGACTTAA GCTTTGCCTT GAGTCTGCTG AGTACATCGA GGAAGACAAT 
CGTCAATCCG ATGCAACTGA ACCTATTTGT CTCAAAGGCT TTCTCAAGCG ACACAGATTT 
CAGATAAAGC GACTTAGCCA GGAACATGTA GATATGGGAG AGGAGAAGCG ATTGCTTGAT 
ATTATTTCGC AGCTGGAACA GGAAATCGAA GAGAAGAGTG CACTTATGGA GGCAACAGAG 
GCAACCATCA ATGAAATGCG AGAGCAGATG ACTAACCTAG AGTCGGCACT CCTCGAAAAG 
AGCGTTATAA TTAACAAGGT TGAGGACTAC CAGCGGCAGA TCGAGTCCCT GGAAAAACAA 
AATGCGGAGA TGACAATGGT ATACGAGGAG CTGCAGGATA GAGTAACAAG GGAGAGCTCG 
ATGAGCGAGA GTCTGCTCCG AGTTCCACCT GACGAGGATA CCCTTCCAGG ATGCCCTACA 
TCTCCCAGTA GACGAGAACA GGAGGTGGCC ACGCTAAAGA CATCCATAAC AGAATTGCAG 
TCACAAGTTT CTGATCTAAA AGCAGAACTT GAAAACCATT TAAGGCAGAT CCAGTTGAAA 
GACGGGAACA TAGCGAGACT ACAGACGGAT TTTGAGGAAA TGAGTGAGCG CTGCTTATCG 
ATGGAGGTGA GGCTAGCCGA GTTGGATGAG GACACTAAGC AAAAACAAGA ACTACTGGAT 
CGTCAGGCGC AGAAGCTCTC CGATGACCTA TGCCTTATCG ATCAGCTTCA AAAGAAAAAC 
GCGCAGCTCG TTGAACAGTA TCATAAAGCG ACAGAATCTC TCAGTCTTGC GGATGCAAAG 
CCGGATCAGA TACTACTTTC CTCCCAATAT GATAGTCAGA TCGAGAAACT TAATCAATTG 
CTAAATGCAG CCAAAGATGA GCTACACGAT GTGCGAAGGA TAAAAGATGA TGAGATAAGT 
GCTTTGCGAA TGGAGTTCCT GCTCCAAATT GAAACGAATG AGAAAGAGAA CCAAGCCAAG 
TTCTACGCTG AATTGCAGGA GACTAAGGAT CGCTATGAGA GCAATGTGGC CGAGTTAAAG 
GAGAAGCTAT TGCAGGTAGA GGAGACACTC TCCAGCGTTA CGGTTCGTTG TCAGGCGGAG 
TTGGAAGCGC TTAAATCTGC TCATAAAGAA AATATTTCTC AGGCTGTGGA GGAAAGAAAC 
AATTTGATTG TCCAGCACCA GGCAGAAATG GAGACTATCA GGGAAACTCT TAAGAATAAG 
CTAGCTGAGG CTAGCACCCA ACAATCAAAA ATGGAAGATG CCTTTAGAGC GGAAATAAAC 
GAGGTGAGAG CCACCCTAAT GGAGCAATTG AATCAAACGA AAGAAGATCG CGATAAGGGA 
GCCTCGAAGC TTGAGGAGGT CAAAAAGACA CTGGAGCAGA TGATAAATGG AGGACGTGTC 
ATGTCAGACA CCATTGCTGA GTTGGAGAAA ACAAAAGCTG AGCAGGACTT GGCAGTCAAT 
AAATTAACAA AGGACAACAT CGAATTAGAG AAGCAGTGCA GTAAGACGCA GGAGCAGTTG 
CAAATGGAAT CGCTTACTCG AGATCAAATC AGTTTCGAAA TAGAAGCACA TATTAAAAAG 
CTGGAATTGA TTGTGGCCTC TTCAAAGAAA AGGATTATTG AGCTCGAGGA AAAGTGTGAC 
CAGCAGGTTC TGGAATTGGA TAAATGTAGG CTCGAGAAGT TGTCCCTGGA ATCAGAGATT 
CAAAAAGCGA ATTCAGAGCA TTCGTGCACC ATGGAAAAAC TTCAGGAACT CCAAGCTGAA 
ATGAAAGTTC TAAGCAACCG GAATGAGAAG GAAAAGTGTG ATTTCGAAAC TAAGCTCGAG 
ACATTTACTT TCAAGATAAC CGATCTCGAA GAAGTCCTGA AGGAGGCACA ACATAAAGTC 
ATTCTCTATG ACGATTTGGT ATCGCAGCAC GAACGCCTAA AAATCTGTCT TGCCGAGGCA 
AATGAACTAT CATCAAACTT GCAAAAGAAA GTGATGAGCC TGCACACGGA ATTGATTGAT 
TCCCAAAAGG GAATATCGAG TCGAGATGTG GAAATTAATG AGCTACGCGA AGAACTGAAG 
GCCGCTATGG ATGCCAAGGC AACCGCGAGT GCCGAACAAA TGACTCTGGT AACGCAGCTC 
AAAGATGTCG AGGAGCGGAT GGCAAACCAG GCAGAAAAGT TCACACGTGA GGCGGCTAAT 
CTAAAAGGGT CTATAAACGA GCTCCTCTTG AAGCTCAATT CCATGCAAGA AACTAAAGAT 
ATGCTAGAGT CCGGAAATGA AGAACTCAAA GAACAGCTGA GAAATTCCCA AAATCTGCGG 
AACATGCTGG ATGAGGAGAG TAAGATGTGT ATCTCCCTAA AAGAAAAATT AGTCAAACTG 
GAAGATGCAA AAACTAGCCT TGAACAGCAG TTGCGAGACA ATAAATCAGA AATATATCAA 
AGGCACACGG AGCTTACCAA GGAGGTGGAG CTGGGCCGCA ACAGAATTGG AGAACTCACA 
AAGAAGTGTG AGGAGCTGTG CTCTGATCTG GAAAACTCAG ACCAGATACG ACTCGATCTG 
CAAGAAACTA AAGAGCAGTT GAAGAAAACG TTGGAAAATA ACCTTGGATG GCAACAGAAG 
GTTGACGAAG TGACAAGAGA GTGCGAAAAG CTACGATTTG ATATGCAATC CAAAGAGGTG 
CAAAATGAAA GTAAGGTTCA GGAATTGATA AGCGAGTGCG AAGAGCTCCG GTCAACTCTG 
AAATCCAAGG AAGCCAGTTT TCAGTCTGAG AAAGAAAGCA TGGACAGAAC AATCTCAAGT 
CTTTTAGAAG ACAAACGCAA CCTCGAGGAA AAGTTGTGCT CGGCTAACGA TATTGTGGCT 
AAGCTTGAGA CGGAAATAGC CGCGTTGCGG CCAAGAAAAA GTCTAGATCG GAACCCAGTG 
CCGAGGAAAT CGATTACCTT TGAATCCGAA ATACGGAAGA ATCGACGAAT CAGCGTCCAC 
GATGAGCGAC GGCAGTCTTA CTGGAACGAC GTTCGGGAAT TTGGCATCAT GACAGATCCC 
GTTGACAACA ACTGCAACTG TGCGGAGCTG AATTCCAAGC TTCAGGACTG CCAGCGTGAG 
CTTTTCATTC GTGAAAGTCA AGTGACGGCA CTAAAGATGG AGCTGGACCA TCATCCGCTG 
AAGGATGAGA ATGCGCAACT AACGAAGCGA GTTATTGAGG AGCAAGACAA GGCAAAGGTT 
GAGCAGAAAC GGCTTAAAAT GAAACTTCAA GATCTTAATG CTAGGATTAA TGACCTCACT 
ACGGCATCAG CCAAGGAACC AGAATCTAAC CAAATGGCAC AAGCAGCTAA ACCTGCTACC 
GTAGCCGCTC AAACTCAGAC GGAATCCGAT CTTGAGACAA TCCTTGAGAA AACGAACGTA 
AAGTATCAGG AAGCGGTTCG CATGTTGCGC TACCGTTACC ATCTCATCCA GGAACTGAAA 
GAGAAACTTA GGCAAAACGA AAACAGTGAT ACCTCGAATA TTACCTCACT CTCAGCTGGA 
CAAACCAGTG CATTGAAGTG TGAGTCCCAA AAGAAGGAAA TTTTGGCAAT CAAATATAAG 
TATGAAGCCG CCAAGCGAAT TTTGGCGATT CGAAATGATG ATTTGGATGC TCTGCGGGAA 
AAACTCGCGA AATATGAAAC AACTACATCA GATAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cmet ( NM_001273451.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260380.1 Drosophila melanogaster CENP-meta (cmet), transcript variant D, mRNA.

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CloneID	OFa15785
Clone ID Related Accession (Same CDS sequence)	NM_001273451.1
Accession Version	NM_001273451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CENP-meta, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CTTATGATGC TCTGGAAAAG GAAGTAACCA GCCTAAGAGC GGATAATGAG 
GCTGCGAATT CAAAAATCAG CGAATTGGAA GAGAAACTGA GTACTCTAAA GCAGACTATG 
AGAATAATGG AAGTGGAAAA TCAGGTGGCC GTGGGCCTCG AGTTTGAGTT TGAGGCTCAC 
AAGAAATCAT CGAAACTTCG CGTGGATGAC TTACTCTCTG CCCTTTTAGA AAAGGAGTCG 
ACTATAGAGA GTCTTCAAAA GTCCCTCGAC AATTTAACCC GCGATGTCTT ACGGAACAGC 
AAGGAGGGCC ACATGCTTTC CATTGCCCCA GAGCAGGAAG ACATCGCCGG CGACAGTATA 
TGCAACAAGT GTGAGGAACT GGAGAAGCTG ATTGCAGATT TAGAGTCCAA GAAAAATTCA 
TGCGAGTGTG ATCAGCTCCG GTTGGAAATC GTCTCTGTTC GCGACAAGTT AGAGAGCGTT 
GAATCTGCCT TTAACCTGGC AAGCTCGGAG ATTATCCAAA AGGCGACTGA TTGCGAGCGA 
CTTTCGAAAG AGCTATCCAC ATCCCAGAAT GCCTTTGGAC AACTTCAGGA AAGGTACGAC 
GCTCTGGACC AACAGTGGCA AGCTCAACAA GCGGGCATCA CAACCCTACA TGAAAAGCAC 
GAGCACGTGC AGGAGAAATA CCAGAAATTG CAGGAGGAGT ATGAGCAGTT AGAAAGCAGG 
GCAAGAAGTG CCTCCAGCGC TGAGTTTCAA CGGCTTCAAA ATGACAACAC GAAGTTTCAA 
GCCGATATCG CATCCCTCAA TGAACGATTG GAGGAGGCCC AAAACATGCT TACGGAAGTC 
CAAAACTCTG AATCTACTGT GGAAAAGCTT CGAATACAAA ATCACGAACT TACAGCGAAA 
ATCAAAGAAC TGGAGACGAA CTTTGAAGAG ATGCAGCGGG AATACGATTG CCTTTCCAAT 
CAACTGATGG AGAGTGTCCA GGAGAACGAT GCCCTGCGAG AGGAGATAAA GCAACGACCC 
ACGAGTCACG TTGAGGAGTC TATGAGGAGT TCCGGTATAA GCTCCGATTT TGACGAACAG 
AAGCAGGATA TAAACCTTCT ACACCAGTTT GTTCAGCTTT CTGAGTCCGT CCAACAGATA 
GAGCTGCAGC ATCATTCCGG CATCAGTCGC CTCTTTAGAG CCAACCAAAT GAAACTGGAT 
CAGAGCGAGC CAGGACTTAA GCTTTGCCTT GAGTCTGCTG AGTACATCGA GGAAGACAAT 
CGTCAATCCG ATGCAACTGA ACCTATTTGT CTCAAAGGCT TTCTCAAGCG ACACAGATTT 
CAGATAAAGC GACTTAGCCA GGAACATGTA GATATGGGAG AGGAGAAGCG ATTGCTTGAT 
ATTATTTCGC AGCTGGAACA GGAAATCGAA GAGAAGAGTG CACTTATGGA GGCAACAGAG 
GCAACCATCA ATGAAATGCG AGAGCAGATG ACTAACCTAG AGTCGGCACT CCTCGAAAAG 
AGCGTTATAA TTAACAAGGT TGAGGACTAC CAGCGGCAGA TCGAGTCCCT GGAAAAACAA 
AATGCGGAGA TGACAATGGT ATACGAGGAG CTGCAGGATA GAGTAACAAG GGAGAGCTCG 
ATGAGCGAGA GTCTGCTCCG AGTTCCACCT GACGAGGATA CCCTTCCAGG ATGCCCTACA 
TCTCCCAGTA GACGAGAACA GGAGGTGGCC ACGCTAAAGA CATCCATAAC AGAATTGCAG 
TCACAAGTTT CTGATCTAAA AGCAGAACTT GAAAACCATT TAAGGCAGAT CCAGTTGAAA 
GACGGGAACA TAGCGAGACT ACAGACGGAT TTTGAGGAAA TGAGTGAGCG CTGCTTATCG 
ATGGAGGTGA GGCTAGCCGA GTTGGATGAG GACACTAAGC AAAAACAAGA ACTACTGGAT 
CGTCAGGCGC AGAAGCTCTC CGATGACCTA TGCCTTATCG ATCAGCTTCA AAAGAAAAAC 
GCGCAGCTCG TTGAACAGTA TCATAAAGCG ACAGAATCTC TCAGTCTTGC GGATGCAAAG 
CCGGATCAGA TACTACTTTC CTCCCAATAT GATAGTCAGA TCGAGAAACT TAATCAATTG 
CTAAATGCAG CCAAAGATGA GCTACACGAT GTGCGAAGGA TAAAAGATGA TGAGATAAGT 
GCTTTGCGAA TGGAGTTCCT GCTCCAAATT GAAACGAATG AGAAAGAGAA CCAAGCCAAG 
TTCTACGCTG AATTGCAGGA GACTAAGGAT CGCTATGAGA GCAATGTGGC CGAGTTAAAG 
GAGAAGCTAT TGCAGGTAGA GGAGACACTC TCCAGCGTTA CGGTTCGTTG TCAGGCGGAG 
TTGGAAGCGC TTAAATCTGC TCATAAAGAA AATATTTCTC AGGCTGTGGA GGAAAGAAAC 
AATTTGATTG TCCAGCACCA GGCAGAAATG GAGACTATCA GGGAAACTCT TAAGAATAAG 
CTAGCTGAGG CTAGCACCCA ACAATCAAAA ATGGAAGATG CCTTTAGAGC GGAAATAAAC 
GAGGTGAGAG CCACCCTAAT GGAGCAATTG AATCAAACGA AAGAAGATCG CGATAAGGGA 
GCCTCGAAGC TTGAGGAGGT CAAAAAGACA CTGGAGCAGA TGATAAATGG AGGACGTGTC 
ATGTCAGACA CCATTGCTGA GTTGGAGAAA ACAAAAGCTG AGCAGGACTT GGCAGTCAAT 
AAATTAACAA AGGACAACAT CGAATTAGAG AAGCAGTGCA GTAAGACGCA GGAGCAGTTG 
CAAATGGAAT CGCTTACTCG AGATCAAATC AGTTTCGAAA TAGAAGCACA TATTAAAAAG 
CTGGAATTGA TTGTGGCCTC TTCAAAGAAA AGGATTATTG AGCTCGAGGA AAAGTGTGAC 
CAGCAGGTTC TGGAATTGGA TAAATGTAGG CTCGAGAAGT TGTCCCTGGA ATCAGAGATT 
CAAAAAGCGA ATTCAGAGCA TTCGTGCACC ATGGAAAAAC TTCAGGAACT CCAAGCTGAA 
ATGAAAGTTC TAAGCAACCG GAATGAGAAG GAAAAGTGTG ATTTCGAAAC TAAGCTCGAG 
ACATTTACTT TCAAGATAAC CGATCTCGAA GAAGTCCTGA AGGAGGCACA ACATAAAGTC 
ATTCTCTATG ACGATTTGGT ATCGCAGCAC GAACGCCTAA AAATCTGTCT TGCCGAGGCA 
AATGAACTAT CATCAAACTT GCAAAAGAAA GTGATGAGCC TGCACACGGA ATTGATTGAT 
TCCCAAAAGG GAATATCGAG TCGAGATGTG GAAATTAATG AGCTACGCGA AGAACTGAAG 
GCCGCTATGG ATGCCAAGGC AACCGCGAGT GCCGAACAAA TGACTCTGGT AACGCAGCTC 
AAAGATGTCG AGGAGCGGAT GGCAAACCAG GCAGAAAAGT TCACACGTGA GGCGGCTAAT 
CTAAAAGGGT CTATAAACGA GCTCCTCTTG AAGCTCAATT CCATGCAAGA AACTAAAGAT 
ATGCTAGAGT CCGGAAATGA AGAACTCAAA GAACAGCTGA GAAATTCCCA AAATCTGCGG 
AACATGCTGG ATGAGGAGAG TAAGATGTGT ATCTCCCTAA AAGAAAAATT AGTCAAACTG 
GAAGATGCAA AAACTAGCCT TGAACAGCAG TTGCGAGACA ATAAATCAGA AATATATCAA 
AGGCACACGG AGCTTACCAA GGAGGTGGAG CTGGGCCGCA ACAGAATTGG AGAACTCACA 
AAGAAGTGTG AGGAGCTGTG CTCTGATCTG GAAAACTCAG ACCAGATACG ACTCGATCTG 
CAAGAAACTA AAGAGCAGTT GAAGAAAACG TTGGAAAATA ACCTTGGATG GCAACAGAAG 
GTTGACGAAG TGACAAGAGA GTGCGAAAAG CTACGATTTG ATATGCAATC CAAAGAGGTG 
CAAAATGAAA GTAAGGTTCA GGAATTGATA AGCGAGTGCG AAGAGCTCCG GTCAACTCTG 
AAATCCAAGG AAGCCAGTTT TCAGTCTGAG AAAGAAAGCA TGGACAGAAC AATCTCAAGT 
CTTTTAGAAG ACAAACGCAA CCTCGAGGAA AAGTTGTGCT CGGCTAACGA TATTGTGGCT 
AAGCTTGAGA CGGAAATAGC CGCGTTGCGG CCAAGAAAAA GTCTAGATCG GAACCCAGTG 
CCGAGGAAAT CGATTACCTT TGAATCCGAA ATACGGAAGA ATCGACGAAT CAGCGTCCAC 
GATGAGCGAC GGCAGTCTTA CTGGAACGAC GTTCGGGAAT TTGGCATCAT GACAGATCCC 
GTTGACAACA ACTGCAACTG TGCGGAGCTG AATTCCAAGC TTCAGGACTG CCAGCGTGAG 
CTTTTCATTC GTGAAAGTCA AGTGACGGCA CTAAAGATGG AGCTGGACCA TCATCCGCTG 
AAGGATGAGA ATGCGCAACT AACGAAGCGA GTTATTGAGG AGCAAGACAA GGCAAAGGTT 
GAGCAGAAAC GGCTTAAAAT GAAACTTCAA GATCTTAATG CTAGGATTAA TGACCTCACT 
ACGGCATCAG CCAAGGAACC AGAATCTAAC CAAATGGCAC AAGCAGCTAA ACCTGCTACC 
GTAGCCGCTC AAACTCAGAC GGAATCCGAT CTTGAGACAA TCCTTGAGAA AACGAACGTA 
AAGTATCAGG AAGCGGTTCG CATGTTGCGC TACCGTTACC ATCTCATCCA GGAACTGAAA 
GAGAAACTTA GGCAAAACGA AAACAGTGAT ACCTCGAATA TTACCTCACT CTCAGCTGGA 
CAAACCAGTG CATTGAAGGC ACAGTGTGAG TCCCAAAAGA AGGAAATTTT GGCAATCAAA 
TATAAGTATG AAGCCGCCAA GCGAATTTTG GCGATTCGAA ATGATGATTT GGATGCTCTG 
CGGGAAAAAC TCGCGAAATA TGAAACAACT ACATCAGATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260380.1
CDS	157..6561
Translation	MSAKNASSIQVCIKVRPCEPGLTSLWQVKERRSIHLADSHAEPYVFDYVFDEGASNQEVFDRMAKHIVHACMQGFNGTIFAYGQTSSGKTYTMMGDEQNPGVMVLAAKEIFQQISSETERDFLLRVGYIEIYNEKIYDLLNKKNQDLKIHESGNGIVNVNCEECIITSEVDLLRLLCLGNKERTVGETNMNERSSRSHAIFKIIIESRKSDHSDDDAVIQSVLNLVDLAGSERADQTGARGARLKEGGHINKSLLFLSNVIKSLSENADNRFTNYRDSKLTRILQASLGGNAFTSIICTIKPSIMEESQSTLSFATRAKKIRIKPQVNEMVSDATMMKRLEREIKVLKDKLAEEERKNENQQKVEHLERQIKHDMHKIICGHSLSDKGQQKRRRTWCPTASGSHLELAETGTTEDRIDQFPKVSHLPKPVFFHTSNAGKRWDNIPKTINILGSLDIGTESNSISEEFLPAECIDFGSPRPDVLKPMLTIRQLPDLPLTPKGPLTYDALEKEVTSLRADNEAANSKISELEEKLSTLKQTMRIMEVENQVAVGLEFEFEAHKKSSKLRVDDLLSALLEKESTIESLQKSLDNLTRDVLRNSKEGHMLSIAPEQEDIAGDSICNKCEELEKLIADLESKKNSCECDQLRLEIVSVRDKLESVESAFNLASSEIIQKATDCERLSKELSTSQNAFGQLQERYDALDQQWQAQQAGITTLHEKHEHVQEKYQKLQEEYEQLESRARSASSAEFQRLQNDNTKFQADIASLNERLEEAQNMLTEVQNSESTVEKLRIQNHELTAKIKELETNFEEMQREYDCLSNQLMESVQENDALREEIKQRPTSHVEESMRSSGISSDFDEQKQDINLLHQFVQLSESVQQIELQHHSGISRLFRANQMKLDQSEPGLKLCLESAEYIEEDNRQSDATEPICLKGFLKRHRFQIKRLSQEHVDMGEEKRLLDIISQLEQEIEEKSALMEATEATINEMREQMTNLESALLEKSVIINKVEDYQRQIESLEKQNAEMTMVYEELQDRVTRESSMSESLLRVPPDEDTLPGCPTSPSRREQEVATLKTSITELQSQVSDLKAELENHLRQIQLKDGNIARLQTDFEEMSERCLSMEVRLAELDEDTKQKQELLDRQAQKLSDDLCLIDQLQKKNAQLVEQYHKATESLSLADAKPDQILLSSQYDSQIEKLNQLLNAAKDELHDVRRIKDDEISALRMEFLLQIETNEKENQAKFYAELQETKDRYESNVAELKEKLLQVEETLSSVTVRCQAELEALKSAHKENISQAVEERNNLIVQHQAEMETIRETLKNKLAEASTQQSKMEDAFRAEINEVRATLMEQLNQTKEDRDKGASKLEEVKKTLEQMINGGRVMSDTIAELEKTKAEQDLAVNKLTKDNIELEKQCSKTQEQLQMESLTRDQISFEIEAHIKKLELIVASSKKRIIELEEKCDQQVLELDKCRLEKLSLESEIQKANSEHSCTMEKLQELQAEMKVLSNRNEKEKCDFETKLETFTFKITDLEEVLKEAQHKVILYDDLVSQHERLKICLAEANELSSNLQKKVMSLHTELIDSQKGISSRDVEINELREELKAAMDAKATASAEQMTLVTQLKDVEERMANQAEKFTREAANLKGSINELLLKLNSMQETKDMLESGNEELKEQLRNSQNLRNMLDEESKMCISLKEKLVKLEDAKTSLEQQLRDNKSEIYQRHTELTKEVELGRNRIGELTKKCEELCSDLENSDQIRLDLQETKEQLKKTLENNLGWQQKVDEVTRECEKLRFDMQSKEVQNESKVQELISECEELRSTLKSKEASFQSEKESMDRTISSLLEDKRNLEEKLCSANDIVAKLETEIAALRPRKSLDRNPVPRKSITFESEIRKNRRISVHDERRQSYWNDVREFGIMTDPVDNNCNCAELNSKLQDCQRELFIRESQVTALKMELDHHPLKDENAQLTKRVIEEQDKAKVEQKRLKMKLQDLNARINDLTTASAKEPESNQMAQAAKPATVAAQTQTESDLETILEKTNVKYQEAVRMLRYRYHLIQELKEKLRQNENSDTSNITSLSAGQTSALKAQCESQKKEILAIKYKYEAAKRILAIRNDDLDALREKLAKYETTTSDK

Target ORF information:

RefSeq Version	NM_001273451.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CENP-meta (cmet), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273451.1

ORF Insert Sequence:

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CTTATGATGC TCTGGAAAAG GAAGTAACCA GCCTAAGAGC GGATAATGAG 
GCTGCGAATT CAAAAATCAG CGAATTGGAA GAGAAACTGA GTACTCTAAA GCAGACTATG 
AGAATAATGG AAGTGGAAAA TCAGGTGGCC GTGGGCCTCG AGTTTGAGTT TGAGGCTCAC 
AAGAAATCAT CGAAACTTCG CGTGGATGAC TTACTCTCTG CCCTTTTAGA AAAGGAGTCG 
ACTATAGAGA GTCTTCAAAA GTCCCTCGAC AATTTAACCC GCGATGTCTT ACGGAACAGC 
AAGGAGGGCC ACATGCTTTC CATTGCCCCA GAGCAGGAAG ACATCGCCGG CGACAGTATA 
TGCAACAAGT GTGAGGAACT GGAGAAGCTG ATTGCAGATT TAGAGTCCAA GAAAAATTCA 
TGCGAGTGTG ATCAGCTCCG GTTGGAAATC GTCTCTGTTC GCGACAAGTT AGAGAGCGTT 
GAATCTGCCT TTAACCTGGC AAGCTCGGAG ATTATCCAAA AGGCGACTGA TTGCGAGCGA 
CTTTCGAAAG AGCTATCCAC ATCCCAGAAT GCCTTTGGAC AACTTCAGGA AAGGTACGAC 
GCTCTGGACC AACAGTGGCA AGCTCAACAA GCGGGCATCA CAACCCTACA TGAAAAGCAC 
GAGCACGTGC AGGAGAAATA CCAGAAATTG CAGGAGGAGT ATGAGCAGTT AGAAAGCAGG 
GCAAGAAGTG CCTCCAGCGC TGAGTTTCAA CGGCTTCAAA ATGACAACAC GAAGTTTCAA 
GCCGATATCG CATCCCTCAA TGAACGATTG GAGGAGGCCC AAAACATGCT TACGGAAGTC 
CAAAACTCTG AATCTACTGT GGAAAAGCTT CGAATACAAA ATCACGAACT TACAGCGAAA 
ATCAAAGAAC TGGAGACGAA CTTTGAAGAG ATGCAGCGGG AATACGATTG CCTTTCCAAT 
CAACTGATGG AGAGTGTCCA GGAGAACGAT GCCCTGCGAG AGGAGATAAA GCAACGACCC 
ACGAGTCACG TTGAGGAGTC TATGAGGAGT TCCGGTATAA GCTCCGATTT TGACGAACAG 
AAGCAGGATA TAAACCTTCT ACACCAGTTT GTTCAGCTTT CTGAGTCCGT CCAACAGATA 
GAGCTGCAGC ATCATTCCGG CATCAGTCGC CTCTTTAGAG CCAACCAAAT GAAACTGGAT 
CAGAGCGAGC CAGGACTTAA GCTTTGCCTT GAGTCTGCTG AGTACATCGA GGAAGACAAT 
CGTCAATCCG ATGCAACTGA ACCTATTTGT CTCAAAGGCT TTCTCAAGCG ACACAGATTT 
CAGATAAAGC GACTTAGCCA GGAACATGTA GATATGGGAG AGGAGAAGCG ATTGCTTGAT 
ATTATTTCGC AGCTGGAACA GGAAATCGAA GAGAAGAGTG CACTTATGGA GGCAACAGAG 
GCAACCATCA ATGAAATGCG AGAGCAGATG ACTAACCTAG AGTCGGCACT CCTCGAAAAG 
AGCGTTATAA TTAACAAGGT TGAGGACTAC CAGCGGCAGA TCGAGTCCCT GGAAAAACAA 
AATGCGGAGA TGACAATGGT ATACGAGGAG CTGCAGGATA GAGTAACAAG GGAGAGCTCG 
ATGAGCGAGA GTCTGCTCCG AGTTCCACCT GACGAGGATA CCCTTCCAGG ATGCCCTACA 
TCTCCCAGTA GACGAGAACA GGAGGTGGCC ACGCTAAAGA CATCCATAAC AGAATTGCAG 
TCACAAGTTT CTGATCTAAA AGCAGAACTT GAAAACCATT TAAGGCAGAT CCAGTTGAAA 
GACGGGAACA TAGCGAGACT ACAGACGGAT TTTGAGGAAA TGAGTGAGCG CTGCTTATCG 
ATGGAGGTGA GGCTAGCCGA GTTGGATGAG GACACTAAGC AAAAACAAGA ACTACTGGAT 
CGTCAGGCGC AGAAGCTCTC CGATGACCTA TGCCTTATCG ATCAGCTTCA AAAGAAAAAC 
GCGCAGCTCG TTGAACAGTA TCATAAAGCG ACAGAATCTC TCAGTCTTGC GGATGCAAAG 
CCGGATCAGA TACTACTTTC CTCCCAATAT GATAGTCAGA TCGAGAAACT TAATCAATTG 
CTAAATGCAG CCAAAGATGA GCTACACGAT GTGCGAAGGA TAAAAGATGA TGAGATAAGT 
GCTTTGCGAA TGGAGTTCCT GCTCCAAATT GAAACGAATG AGAAAGAGAA CCAAGCCAAG 
TTCTACGCTG AATTGCAGGA GACTAAGGAT CGCTATGAGA GCAATGTGGC CGAGTTAAAG 
GAGAAGCTAT TGCAGGTAGA GGAGACACTC TCCAGCGTTA CGGTTCGTTG TCAGGCGGAG 
TTGGAAGCGC TTAAATCTGC TCATAAAGAA AATATTTCTC AGGCTGTGGA GGAAAGAAAC 
AATTTGATTG TCCAGCACCA GGCAGAAATG GAGACTATCA GGGAAACTCT TAAGAATAAG 
CTAGCTGAGG CTAGCACCCA ACAATCAAAA ATGGAAGATG CCTTTAGAGC GGAAATAAAC 
GAGGTGAGAG CCACCCTAAT GGAGCAATTG AATCAAACGA AAGAAGATCG CGATAAGGGA 
GCCTCGAAGC TTGAGGAGGT CAAAAAGACA CTGGAGCAGA TGATAAATGG AGGACGTGTC 
ATGTCAGACA CCATTGCTGA GTTGGAGAAA ACAAAAGCTG AGCAGGACTT GGCAGTCAAT 
AAATTAACAA AGGACAACAT CGAATTAGAG AAGCAGTGCA GTAAGACGCA GGAGCAGTTG 
CAAATGGAAT CGCTTACTCG AGATCAAATC AGTTTCGAAA TAGAAGCACA TATTAAAAAG 
CTGGAATTGA TTGTGGCCTC TTCAAAGAAA AGGATTATTG AGCTCGAGGA AAAGTGTGAC 
CAGCAGGTTC TGGAATTGGA TAAATGTAGG CTCGAGAAGT TGTCCCTGGA ATCAGAGATT 
CAAAAAGCGA ATTCAGAGCA TTCGTGCACC ATGGAAAAAC TTCAGGAACT CCAAGCTGAA 
ATGAAAGTTC TAAGCAACCG GAATGAGAAG GAAAAGTGTG ATTTCGAAAC TAAGCTCGAG 
ACATTTACTT TCAAGATAAC CGATCTCGAA GAAGTCCTGA AGGAGGCACA ACATAAAGTC 
ATTCTCTATG ACGATTTGGT ATCGCAGCAC GAACGCCTAA AAATCTGTCT TGCCGAGGCA 
AATGAACTAT CATCAAACTT GCAAAAGAAA GTGATGAGCC TGCACACGGA ATTGATTGAT 
TCCCAAAAGG GAATATCGAG TCGAGATGTG GAAATTAATG AGCTACGCGA AGAACTGAAG 
GCCGCTATGG ATGCCAAGGC AACCGCGAGT GCCGAACAAA TGACTCTGGT AACGCAGCTC 
AAAGATGTCG AGGAGCGGAT GGCAAACCAG GCAGAAAAGT TCACACGTGA GGCGGCTAAT 
CTAAAAGGGT CTATAAACGA GCTCCTCTTG AAGCTCAATT CCATGCAAGA AACTAAAGAT 
ATGCTAGAGT CCGGAAATGA AGAACTCAAA GAACAGCTGA GAAATTCCCA AAATCTGCGG 
AACATGCTGG ATGAGGAGAG TAAGATGTGT ATCTCCCTAA AAGAAAAATT AGTCAAACTG 
GAAGATGCAA AAACTAGCCT TGAACAGCAG TTGCGAGACA ATAAATCAGA AATATATCAA 
AGGCACACGG AGCTTACCAA GGAGGTGGAG CTGGGCCGCA ACAGAATTGG AGAACTCACA 
AAGAAGTGTG AGGAGCTGTG CTCTGATCTG GAAAACTCAG ACCAGATACG ACTCGATCTG 
CAAGAAACTA AAGAGCAGTT GAAGAAAACG TTGGAAAATA ACCTTGGATG GCAACAGAAG 
GTTGACGAAG TGACAAGAGA GTGCGAAAAG CTACGATTTG ATATGCAATC CAAAGAGGTG 
CAAAATGAAA GTAAGGTTCA GGAATTGATA AGCGAGTGCG AAGAGCTCCG GTCAACTCTG 
AAATCCAAGG AAGCCAGTTT TCAGTCTGAG AAAGAAAGCA TGGACAGAAC AATCTCAAGT 
CTTTTAGAAG ACAAACGCAA CCTCGAGGAA AAGTTGTGCT CGGCTAACGA TATTGTGGCT 
AAGCTTGAGA CGGAAATAGC CGCGTTGCGG CCAAGAAAAA GTCTAGATCG GAACCCAGTG 
CCGAGGAAAT CGATTACCTT TGAATCCGAA ATACGGAAGA ATCGACGAAT CAGCGTCCAC 
GATGAGCGAC GGCAGTCTTA CTGGAACGAC GTTCGGGAAT TTGGCATCAT GACAGATCCC 
GTTGACAACA ACTGCAACTG TGCGGAGCTG AATTCCAAGC TTCAGGACTG CCAGCGTGAG 
CTTTTCATTC GTGAAAGTCA AGTGACGGCA CTAAAGATGG AGCTGGACCA TCATCCGCTG 
AAGGATGAGA ATGCGCAACT AACGAAGCGA GTTATTGAGG AGCAAGACAA GGCAAAGGTT 
GAGCAGAAAC GGCTTAAAAT GAAACTTCAA GATCTTAATG CTAGGATTAA TGACCTCACT 
ACGGCATCAG CCAAGGAACC AGAATCTAAC CAAATGGCAC AAGCAGCTAA ACCTGCTACC 
GTAGCCGCTC AAACTCAGAC GGAATCCGAT CTTGAGACAA TCCTTGAGAA AACGAACGTA 
AAGTATCAGG AAGCGGTTCG CATGTTGCGC TACCGTTACC ATCTCATCCA GGAACTGAAA 
GAGAAACTTA GGCAAAACGA AAACAGTGAT ACCTCGAATA TTACCTCACT CTCAGCTGGA 
CAAACCAGTG CATTGAAGGC ACAGTGTGAG TCCCAAAAGA AGGAAATTTT GGCAATCAAA 
TATAAGTATG AAGCCGCCAA GCGAATTTTG GCGATTCGAA ATGATGATTT GGATGCTCTG 
CGGGAAAAAC TCGCGAAATA TGAAACAACT ACATCAGATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cmet ( NM_001273452.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260381.1 Drosophila melanogaster CENP-meta (cmet), transcript variant E, mRNA.

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CloneID	OFa15786
Clone ID Related Accession (Same CDS sequence)	NM_001273452.1
Accession Version	NM_001273452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	CENP-meta, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CAACGGATAA AATCAAGAAG GAAATCCAGG ACCTGCAAAT GTTCACCAGC 
TTAGAGAAGC ATTTTGAGGT GGAGTGCGAA GAGGTGCAGG GTTTAAAAGA AAAGCTGGCC 
GAGGTTACAG CACAGCGGGA TAATTTAGAG CAAGAATCGT TAGCCGAAAA GGAGCGTTAT 
GATGCTCTGG AAAAGGAAGT AACCAGCCTA AGAGCGGATA ATGAGGCTGC GAATTCAAAA 
ATCAGCGAAT TGGAAGAGAA ACTGAGTACT CTAAAGCAGA CTATGAGAAT AATGGAAGTG 
GAAAATCAGG TGGCCGTGGG CCTCGAGTTT GAGTTTGAGG CTCACAAGAA ATCATCGAAA 
CTTCGCGTGG ATGACTTACT CTCTGCCCTT TTAGAAAAGG AGTCGACTAT AGAGAGTCTT 
CAAAAGTCCC TCGACAATTT AACCCGCGAT GTCTTACGGA ACAGCAAGGA GGGCCACATG 
CTTTCCATTG CCCCAGAGCA GGAAGACATC GCCGGCGACA GTATATGCAA CAAGTGTGAG 
GAACTGGAGA AGCTGATTGC AGATTTAGAG TCCAAGAAAA ATTCATGCGA GTGTGATCAG 
CTCCGGTTGG AAATCGTCTC TGTTCGCGAC AAGTTAGAGA GCGTTGAATC TGCCTTTAAC 
CTGGCAAGCT CGGAGATTAT CCAAAAGGCG ACTGATTGCG AGCGACTTTC GAAAGAGCTA 
TCCACATCCC AGAATGCCTT TGGACAACTT CAGGAAAGGT ACGACGCTCT GGACCAACAG 
TGGCAAGCTC AACAAGCGGG CATCACAACC CTACATGAAA AGCACGAGCA CGTGCAGGAG 
AAATACCAGA AATTGCAGGA GGAGTATGAG CAGTTAGAAA GCAGGGCAAG AAGTGCCTCC 
AGCGCTGAGT TTCAACGGCT TCAAAATGAC AACACGAAGT TTCAAGCCGA TATCGCATCC 
CTCAATGAAC GATTGGAGGA GGCCCAAAAC ATGCTTACGG AAGTCCAAAA CTCTGAATCT 
ACTGTGGAAA AGCTTCGAAT ACAAAATCAC GAACTTACAG CGAAAATCAA AGAACTGGAG 
ACGAACTTTG AAGAGATGCA GCGGGAATAC GATTGCCTTT CCAATCAACT GATGGAGAGT 
GTCCAGGAGA ACGATGCCCT GCGAGAGGAG ATAAAGCAAC GACCCACGAG TCACGTTGAG 
GAGTCTATGA GGAGTTCCGG TATAAGCTCC GATTTTGACG AACAGAAGCA GGATATAAAC 
CTTCTACACC AGTTTGTTCA GCTTTCTGAG TCCGTCCAAC AGATAGAGCT GCAGCATCAT 
TCCGGCATCA GTCGCCTCTT TAGAGCCAAC CAAATGAAAC TGGATCAGAG CGAGCCAGGA 
CTTAAGCTTT GCCTTGAGTC TGCTGAGTAC ATCGAGGAAG ACAATCGTCA ATCCGATGCA 
ACTGAACCTA TTTGTCTCAA AGGCTTTCTC AAGCGACACA GATTTCAGAT AAAGCGACTT 
AGCCAGGAAC ATGTAGATAT GGGAGAGGAG AAGCGATTGC TTGATATTAT TTCGCAGCTG 
GAACAGGAAA TCGAAGAGAA GAGTGCACTT ATGGAGGCAA CAGAGGCAAC CATCAATGAA 
ATGCGAGAGC AGATGACTAA CCTAGAGTCG GCACTCCTCG AAAAGAGCGT TATAATTAAC 
AAGGTTGAGG ACTACCAGCG GCAGATCGAG TCCCTGGAAA AACAAAATGC GGAGATGACA 
ATGGTATACG AGGAGCTGCA GGATAGAGTA ACAAGGGAGA GCTCGATGAG CGAGAGTCTG 
CTCCGAGTTC CACCTGACGA GGATACCCTT CCAGGATGCC CTACATCTCC CAGTAGACGA 
GAACAGGAGG TGGCCACGCT AAAGACATCC ATAACAGAAT TGCAGTCACA AGTTTCTGAT 
CTAAAAGCAG AACTTGAAAA CCATTTAAGG CAGATCCAGT TGAAAGACGG GAACATAGCG 
AGACTACAGA CGGATTTTGA GGAAATGAGT GAGCGCTGCT TATCGATGGA GGTGAGGCTA 
GCCGAGTTGG ATGAGGACAC TAAGCAAAAA CAAGAACTAC TGGATCGTCA GGCGCAGAAG 
CTCTCCGATG ACCTATGCCT TATCGATCAG CTTCAAAAGA AAAACGCGCA GCTCGTTGAA 
CAGTATCATA AAGCGACAGA ATCTCTCAGT CTTGCGGATG CAAAGCCGGA TCAGATACTA 
CTTTCCTCCC AATATGATAG TCAGATCGAG AAACTTAATC AATTGCTAAA TGCAGCCAAA 
GATGAGCTAC ACGATGTGCG AAGGATAAAA GATGATGAGA TAAGTGCTTT GCGAATGGAG 
TTCCTGCTCC AAATTGAAAC GAATGAGAAA GAGAACCAAG CCAAGTTCTA CGCTGAATTG 
CAGGAGACTA AGGATCGCTA TGAGAGCAAT GTGGCCGAGT TAAAGGAGAA GCTATTGCAG 
GTAGAGGAGA CACTCTCCAG CGTTACGGTT CGTTGTCAGG CGGAGTTGGA AGCGCTTAAA 
TCTGCTCATA AAGAAAATAT TTCTCAGGCT GTGGAGGAAA GAAACAATTT GATTGTCCAG 
CACCAGGCAG AAATGGAGAC TATCAGGGAA ACTCTTAAGA ATAAGCTAGC TGAGGCTAGC 
ACCCAACAAT CAAAAATGGA AGATGCCTTT AGAGCGGAAA TAAACGAGGT GAGAGCCACC 
CTAATGGAGC AATTGAATCA AACGAAAGAA GATCGCGATA AGGGAGCCTC GAAGCTTGAG 
GAGGTCAAAA AGACACTGGA GCAGATGATA AATGGAGGAC GTGTCATGTC AGACACCATT 
GCTGAGTTGG AGAAAACAAA AGCTGAGCAG GACTTGGCAG TCAATAAATT AACAAAGGAC 
AACATCGAAT TAGAGAAGCA GTGCAGTAAG ACGCAGGAGC AGTTGCAAAT GGAATCGCTT 
ACTCGAGATC AAATCAGTTT CGAAATAGAA GCACATATTA AAAAGCTGGA ATTGATTGTG 
GCCTCTTCAA AGAAAAGGAT TATTGAGCTC GAGGAAAAGT GTGACCAGCA GGTTCTGGAA 
TTGGATAAAT GTAGGCTCGA GAAGTTGTCC CTGGAATCAG AGATTCAAAA AGCGAATTCA 
GAGCATTCGT GCACCATGGA AAAACTTCAG GAACTCCAAG CTGAAATGAA AGTTCTAAGC 
AACCGGAATG AGAAGGAAAA GTGTGATTTC GAAACTAAGC TCGAGACATT TACTTTCAAG 
ATAACCGATC TCGAAGAAGT CCTGAAGGAG GCACAACATA AAGTCATTCT CTATGACGAT 
TTGGTATCGC AGCACGAACG CCTAAAAATC TGTCTTGCCG AGGCAAATGA ACTATCATCA 
AACTTGCAAA AGAAAGTGAT GAGCCTGCAC ACGGAATTGA TTGATTCCCA AAAGGGAATA 
TCGAGTCGAG ATGTGGAAAT TAATGAGCTA CGCGAAGAAC TGAAGGCCGC TATGGATGCC 
AAGGCAACCG CGAGTGCCGA ACAAATGACT CTGGTAACGC AGCTCAAAGA TGTCGAGGAG 
CGGATGGCAA ACCAGGCAGA AAAGTTCACA CGTGAGGCGG CTAATCTAAA AGGGTCTATA 
AACGAGCTCC TCTTGAAGCT CAATTCCATG CAAGAAACTA AAGATATGCT AGAGTCCGGA 
AATGAAGAAC TCAAAGAACA GCTGAGAAAT TCCCAAAATC TGCGGAACAT GCTGGATGAG 
GAGAGTAAGA TGTGTATCTC CCTAAAAGAA AAATTAGTCA AACTGGAAGA TGCAAAAACT 
AGCCTTGAAC AGCAGTTGCG AGACAATAAA TCAGAAATAT ATCAAAGGCA CACGGAGCTT 
ACCAAGGAGG TGGAGCTGGG CCGCAACAGA ATTGGAGAAC TCACAAAGAA GTGTGAGGAG 
CTGTGCTCTG ATCTGGAAAA CTCAGACCAG ATACGACTCG ATCTGCAAGA AACTAAAGAG 
CAGTTGAAGA AAACGTTGGA AAATAACCTT GGATGGCAAC AGAAGGTTGA CGAAGTGACA 
AGAGAGTGCG AAAAGCTACG ATTTGATATG CAATCCAAAG AGGTGCAAAA TGAAAGTAAG 
GTTCAGGAAT TGATAAGCGA GTGCGAAGAG CTCCGGTCAA CTCTGGAAGC CAGTTTTCAG 
TCTGAGAAAG AAAGCATGGA CAGAACAATC TCAAGTCTTT TAGAAGACAA ACGCAACCTC 
GAGGAAAAGT TGTGCTCGGC TAACGATATT GTGGCTAAGC TTGAGACGGA AATAGCCGCG 
TTGCGGCCAA GAAAAAGTCT AGATCGGAAC CCAGTGCCGA GGAAATCGAT TACCTTTGAA 
TCCGAAATAC GGAAGAATCG ACGAATCAGC GTCCACGATG AGCGACGGCA GTCTTACTGG 
AACGACGTTC GGGAATTTGG CATCATGACA GATCCCGTTG ACAACAACTG CAACTGTGCG 
GAGCTGAATT CCAAGCTTCA GGACTGCCAG CGTGAGCTTT TCATTCGTGA AAGTCAAGTG 
ACGGCACTAA AGATGGAGCT GGACCATCAT CCGCTGAAGG ATGAGAATGC GCAACTAACG 
AAGCGAGTTA TTGAGGAGCA AGACAAGGCA AAGGTTGAGC AGAAACGGCT TAAAATGAAA 
CTTCAAGATC TTAATGCTAG GATTAATGAC CTCACTACGG CATCAGCCAA GGAACCAGAA 
TCTAACCAAA TGGCACAAGC AGCTAAACCT GCTACCGTAG CCGCTCAAAC TCAGACGGAA 
TCCGATCTTG AGACAATCCT TGAGAAAACG AACGTAAAGT ATCAGGAAGC GGTTCGCATG 
TTGCGCTACC GTTACCATCT CATCCAGGAA CTGAAAGAGA AACTTAGGCA AAACGAAAAC 
AGTGATACCT CGAATATTAC CTCACTCTCA GCTGGACAAA CCAGTGCATT GAAGGCACAG 
TGTGAGTCCC AAAAGAAGGA AATTTTGGCA ATCAAATATA AGTATGAAGC CGCCAAGCGA 
ATTTTGGCGA TTCGAAATGA TGATTTGGAT GCTCTGCGGG AAAAACTCGC GAAATATGAA 
ACAACTACAT CAGATAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260381.1
CDS	157..6717
Translation	MSAKNASSIQVCIKVRPCEPGLTSLWQVKERRSIHLADSHAEPYVFDYVFDEGASNQEVFDRMAKHIVHACMQGFNGTIFAYGQTSSGKTYTMMGDEQNPGVMVLAAKEIFQQISSETERDFLLRVGYIEIYNEKIYDLLNKKNQDLKIHESGNGIVNVNCEECIITSEVDLLRLLCLGNKERTVGETNMNERSSRSHAIFKIIIESRKSDHSDDDAVIQSVLNLVDLAGSERADQTGARGARLKEGGHINKSLLFLSNVIKSLSENADNRFTNYRDSKLTRILQASLGGNAFTSIICTIKPSIMEESQSTLSFATRAKKIRIKPQVNEMVSDATMMKRLEREIKVLKDKLAEEERKNENQQKVEHLERQIKHDMHKIICGHSLSDKGQQKRRRTWCPTASGSHLELAETGTTEDRIDQFPKVSHLPKPVFFHTSNAGKRWDNIPKTINILGSLDIGTESNSISEEFLPAECIDFGSPRPDVLKPMLTIRQLPDLPLTPKGPLTTDKIKKEIQDLQMFTSLEKHFEVECEEVQGLKEKLAEVTAQRDNLEQESLAEKERYDALEKEVTSLRADNEAANSKISELEEKLSTLKQTMRIMEVENQVAVGLEFEFEAHKKSSKLRVDDLLSALLEKESTIESLQKSLDNLTRDVLRNSKEGHMLSIAPEQEDIAGDSICNKCEELEKLIADLESKKNSCECDQLRLEIVSVRDKLESVESAFNLASSEIIQKATDCERLSKELSTSQNAFGQLQERYDALDQQWQAQQAGITTLHEKHEHVQEKYQKLQEEYEQLESRARSASSAEFQRLQNDNTKFQADIASLNERLEEAQNMLTEVQNSESTVEKLRIQNHELTAKIKELETNFEEMQREYDCLSNQLMESVQENDALREEIKQRPTSHVEESMRSSGISSDFDEQKQDINLLHQFVQLSESVQQIELQHHSGISRLFRANQMKLDQSEPGLKLCLESAEYIEEDNRQSDATEPICLKGFLKRHRFQIKRLSQEHVDMGEEKRLLDIISQLEQEIEEKSALMEATEATINEMREQMTNLESALLEKSVIINKVEDYQRQIESLEKQNAEMTMVYEELQDRVTRESSMSESLLRVPPDEDTLPGCPTSPSRREQEVATLKTSITELQSQVSDLKAELENHLRQIQLKDGNIARLQTDFEEMSERCLSMEVRLAELDEDTKQKQELLDRQAQKLSDDLCLIDQLQKKNAQLVEQYHKATESLSLADAKPDQILLSSQYDSQIEKLNQLLNAAKDELHDVRRIKDDEISALRMEFLLQIETNEKENQAKFYAELQETKDRYESNVAELKEKLLQVEETLSSVTVRCQAELEALKSAHKENISQAVEERNNLIVQHQAEMETIRETLKNKLAEASTQQSKMEDAFRAEINEVRATLMEQLNQTKEDRDKGASKLEEVKKTLEQMINGGRVMSDTIAELEKTKAEQDLAVNKLTKDNIELEKQCSKTQEQLQMESLTRDQISFEIEAHIKKLELIVASSKKRIIELEEKCDQQVLELDKCRLEKLSLESEIQKANSEHSCTMEKLQELQAEMKVLSNRNEKEKCDFETKLETFTFKITDLEEVLKEAQHKVILYDDLVSQHERLKICLAEANELSSNLQKKVMSLHTELIDSQKGISSRDVEINELREELKAAMDAKATASAEQMTLVTQLKDVEERMANQAEKFTREAANLKGSINELLLKLNSMQETKDMLESGNEELKEQLRNSQNLRNMLDEESKMCISLKEKLVKLEDAKTSLEQQLRDNKSEIYQRHTELTKEVELGRNRIGELTKKCEELCSDLENSDQIRLDLQETKEQLKKTLENNLGWQQKVDEVTRECEKLRFDMQSKEVQNESKVQELISECEELRSTLEASFQSEKESMDRTISSLLEDKRNLEEKLCSANDIVAKLETEIAALRPRKSLDRNPVPRKSITFESEIRKNRRISVHDERRQSYWNDVREFGIMTDPVDNNCNCAELNSKLQDCQRELFIRESQVTALKMELDHHPLKDENAQLTKRVIEEQDKAKVEQKRLKMKLQDLNARINDLTTASAKEPESNQMAQAAKPATVAAQTQTESDLETILEKTNVKYQEAVRMLRYRYHLIQELKEKLRQNENSDTSNITSLSAGQTSALKAQCESQKKEILAIKYKYEAAKRILAIRNDDLDALREKLAKYETTTSDK

Target ORF information:

RefSeq Version	NM_001273452.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster CENP-meta (cmet), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273452.1

ORF Insert Sequence:

ATGTCTGCGA AGAACGCCAG CTCCATCCAG GTCTGCATCA AGGTGCGTCC CTGCGAGCCC 
GGGCTCACTT CACTCTGGCA GGTGAAGGAG CGCCGCTCCA TCCACTTGGC GGACAGCCAT 
GCGGAGCCCT ATGTCTTCGA CTATGTGTTC GACGAGGGCG CCAGCAACCA GGAGGTGTTC 
GACCGGATGG CCAAGCACAT AGTGCACGCC TGCATGCAGG GCTTCAACGG AACTATTTTT 
GCCTACGGCC AGACGTCGTC GGGCAAGACG TACACCATGA TGGGCGACGA ACAGAATCCG 
GGCGTCATGG TGCTAGCCGC CAAGGAGATC TTTCAACAGA TCTCCAGTGA GACGGAGCGG 
GACTTTCTGC TGCGCGTGGG CTACATCGAG ATCTACAACG AGAAGATCTA CGATCTGCTG 
AACAAGAAGA ATCAGGACCT CAAGATCCAT GAGTCCGGAA ATGGGATTGT GAATGTGAAC 
TGCGAGGAGT GCATCATAAC CAGCGAGGTT GACCTCCTGC GCCTGCTATG CTTGGGCAAT 
AAGGAGCGCA CCGTGGGCGA GACGAACATG AACGAGCGGT CCAGCCGTTC GCATGCCATA 
TTTAAGATAA TCATCGAGTC CCGAAAGTCG GACCACAGCG ATGACGACGC AGTGATTCAA 
AGCGTGTTGA ACCTGGTGGA TCTGGCTGGG TCAGAGCGGG CGGACCAAAC GGGTGCTCGA 
GGAGCGCGCC TCAAAGAGGG CGGTCATATA AACAAGAGCT TACTTTTTCT CAGTAACGTT 
ATCAAAAGTC TGTCGGAAAA CGCGGACAAT AGATTTACCA ACTACCGCGA TTCCAAGCTG 
ACGCGCATTC TGCAGGCTTC TTTGGGCGGC AATGCATTCA CATCCATCAT TTGCACAATC 
AAACCATCGA TCATGGAAGA GTCGCAGTCT ACCCTTAGTT TCGCCACGCG AGCCAAGAAA 
ATTCGCATTA AGCCCCAAGT CAACGAGATG GTATCTGACG CCACAATGAT GAAGCGGCTG 
GAGCGAGAAA TAAAGGTGCT TAAGGATAAG CTGGCCGAAG AAGAGCGCAA AAACGAGAAC 
CAGCAAAAGG TAGAGCATCT GGAGAGACAA ATAAAACACG ACATGCACAA GATAATCTGT 
GGTCACTCCC TGAGCGACAA GGGTCAGCAG AAGCGTCGCC GTACTTGGTG TCCCACTGCA 
TCGGGATCGC ATTTGGAGCT AGCAGAGACC GGCACAACTG AAGATCGAAT TGACCAATTT 
CCAAAGGTCT CACACCTTCC AAAACCAGTG TTTTTTCACA CTTCAAATGC AGGCAAACGA 
TGGGACAACA TTCCAAAGAC CATAAACATT TTGGGTTCCC TGGATATCGG TACCGAGAGC 
AACTCCATTA GTGAGGAATT TCTCCCGGCA GAATGCATTG ATTTTGGCTC CCCTCGCCCA 
GATGTCCTCA AACCCATGTT GACCATCAGG CAACTGCCAG ACCTGCCCCT CACTCCCAAG 
GGTCCCTTAA CAACGGATAA AATCAAGAAG GAAATCCAGG ACCTGCAAAT GTTCACCAGC 
TTAGAGAAGC ATTTTGAGGT GGAGTGCGAA GAGGTGCAGG GTTTAAAAGA AAAGCTGGCC 
GAGGTTACAG CACAGCGGGA TAATTTAGAG CAAGAATCGT TAGCCGAAAA GGAGCGTTAT 
GATGCTCTGG AAAAGGAAGT AACCAGCCTA AGAGCGGATA ATGAGGCTGC GAATTCAAAA 
ATCAGCGAAT TGGAAGAGAA ACTGAGTACT CTAAAGCAGA CTATGAGAAT AATGGAAGTG 
GAAAATCAGG TGGCCGTGGG CCTCGAGTTT GAGTTTGAGG CTCACAAGAA ATCATCGAAA 
CTTCGCGTGG ATGACTTACT CTCTGCCCTT TTAGAAAAGG AGTCGACTAT AGAGAGTCTT 
CAAAAGTCCC TCGACAATTT AACCCGCGAT GTCTTACGGA ACAGCAAGGA GGGCCACATG 
CTTTCCATTG CCCCAGAGCA GGAAGACATC GCCGGCGACA GTATATGCAA CAAGTGTGAG 
GAACTGGAGA AGCTGATTGC AGATTTAGAG TCCAAGAAAA ATTCATGCGA GTGTGATCAG 
CTCCGGTTGG AAATCGTCTC TGTTCGCGAC AAGTTAGAGA GCGTTGAATC TGCCTTTAAC 
CTGGCAAGCT CGGAGATTAT CCAAAAGGCG ACTGATTGCG AGCGACTTTC GAAAGAGCTA 
TCCACATCCC AGAATGCCTT TGGACAACTT CAGGAAAGGT ACGACGCTCT GGACCAACAG 
TGGCAAGCTC AACAAGCGGG CATCACAACC CTACATGAAA AGCACGAGCA CGTGCAGGAG 
AAATACCAGA AATTGCAGGA GGAGTATGAG CAGTTAGAAA GCAGGGCAAG AAGTGCCTCC 
AGCGCTGAGT TTCAACGGCT TCAAAATGAC AACACGAAGT TTCAAGCCGA TATCGCATCC 
CTCAATGAAC GATTGGAGGA GGCCCAAAAC ATGCTTACGG AAGTCCAAAA CTCTGAATCT 
ACTGTGGAAA AGCTTCGAAT ACAAAATCAC GAACTTACAG CGAAAATCAA AGAACTGGAG 
ACGAACTTTG AAGAGATGCA GCGGGAATAC GATTGCCTTT CCAATCAACT GATGGAGAGT 
GTCCAGGAGA ACGATGCCCT GCGAGAGGAG ATAAAGCAAC GACCCACGAG TCACGTTGAG 
GAGTCTATGA GGAGTTCCGG TATAAGCTCC GATTTTGACG AACAGAAGCA GGATATAAAC 
CTTCTACACC AGTTTGTTCA GCTTTCTGAG TCCGTCCAAC AGATAGAGCT GCAGCATCAT 
TCCGGCATCA GTCGCCTCTT TAGAGCCAAC CAAATGAAAC TGGATCAGAG CGAGCCAGGA 
CTTAAGCTTT GCCTTGAGTC TGCTGAGTAC ATCGAGGAAG ACAATCGTCA ATCCGATGCA 
ACTGAACCTA TTTGTCTCAA AGGCTTTCTC AAGCGACACA GATTTCAGAT AAAGCGACTT 
AGCCAGGAAC ATGTAGATAT GGGAGAGGAG AAGCGATTGC TTGATATTAT TTCGCAGCTG 
GAACAGGAAA TCGAAGAGAA GAGTGCACTT ATGGAGGCAA CAGAGGCAAC CATCAATGAA 
ATGCGAGAGC AGATGACTAA CCTAGAGTCG GCACTCCTCG AAAAGAGCGT TATAATTAAC 
AAGGTTGAGG ACTACCAGCG GCAGATCGAG TCCCTGGAAA AACAAAATGC GGAGATGACA 
ATGGTATACG AGGAGCTGCA GGATAGAGTA ACAAGGGAGA GCTCGATGAG CGAGAGTCTG 
CTCCGAGTTC CACCTGACGA GGATACCCTT CCAGGATGCC CTACATCTCC CAGTAGACGA 
GAACAGGAGG TGGCCACGCT AAAGACATCC ATAACAGAAT TGCAGTCACA AGTTTCTGAT 
CTAAAAGCAG AACTTGAAAA CCATTTAAGG CAGATCCAGT TGAAAGACGG GAACATAGCG 
AGACTACAGA CGGATTTTGA GGAAATGAGT GAGCGCTGCT TATCGATGGA GGTGAGGCTA 
GCCGAGTTGG ATGAGGACAC TAAGCAAAAA CAAGAACTAC TGGATCGTCA GGCGCAGAAG 
CTCTCCGATG ACCTATGCCT TATCGATCAG CTTCAAAAGA AAAACGCGCA GCTCGTTGAA 
CAGTATCATA AAGCGACAGA ATCTCTCAGT CTTGCGGATG CAAAGCCGGA TCAGATACTA 
CTTTCCTCCC AATATGATAG TCAGATCGAG AAACTTAATC AATTGCTAAA TGCAGCCAAA 
GATGAGCTAC ACGATGTGCG AAGGATAAAA GATGATGAGA TAAGTGCTTT GCGAATGGAG 
TTCCTGCTCC AAATTGAAAC GAATGAGAAA GAGAACCAAG CCAAGTTCTA CGCTGAATTG 
CAGGAGACTA AGGATCGCTA TGAGAGCAAT GTGGCCGAGT TAAAGGAGAA GCTATTGCAG 
GTAGAGGAGA CACTCTCCAG CGTTACGGTT CGTTGTCAGG CGGAGTTGGA AGCGCTTAAA 
TCTGCTCATA AAGAAAATAT TTCTCAGGCT GTGGAGGAAA GAAACAATTT GATTGTCCAG 
CACCAGGCAG AAATGGAGAC TATCAGGGAA ACTCTTAAGA ATAAGCTAGC TGAGGCTAGC 
ACCCAACAAT CAAAAATGGA AGATGCCTTT AGAGCGGAAA TAAACGAGGT GAGAGCCACC 
CTAATGGAGC AATTGAATCA AACGAAAGAA GATCGCGATA AGGGAGCCTC GAAGCTTGAG 
GAGGTCAAAA AGACACTGGA GCAGATGATA AATGGAGGAC GTGTCATGTC AGACACCATT 
GCTGAGTTGG AGAAAACAAA AGCTGAGCAG GACTTGGCAG TCAATAAATT AACAAAGGAC 
AACATCGAAT TAGAGAAGCA GTGCAGTAAG ACGCAGGAGC AGTTGCAAAT GGAATCGCTT 
ACTCGAGATC AAATCAGTTT CGAAATAGAA GCACATATTA AAAAGCTGGA ATTGATTGTG 
GCCTCTTCAA AGAAAAGGAT TATTGAGCTC GAGGAAAAGT GTGACCAGCA GGTTCTGGAA 
TTGGATAAAT GTAGGCTCGA GAAGTTGTCC CTGGAATCAG AGATTCAAAA AGCGAATTCA 
GAGCATTCGT GCACCATGGA AAAACTTCAG GAACTCCAAG CTGAAATGAA AGTTCTAAGC 
AACCGGAATG AGAAGGAAAA GTGTGATTTC GAAACTAAGC TCGAGACATT TACTTTCAAG 
ATAACCGATC TCGAAGAAGT CCTGAAGGAG GCACAACATA AAGTCATTCT CTATGACGAT 
TTGGTATCGC AGCACGAACG CCTAAAAATC TGTCTTGCCG AGGCAAATGA ACTATCATCA 
AACTTGCAAA AGAAAGTGAT GAGCCTGCAC ACGGAATTGA TTGATTCCCA AAAGGGAATA 
TCGAGTCGAG ATGTGGAAAT TAATGAGCTA CGCGAAGAAC TGAAGGCCGC TATGGATGCC 
AAGGCAACCG CGAGTGCCGA ACAAATGACT CTGGTAACGC AGCTCAAAGA TGTCGAGGAG 
CGGATGGCAA ACCAGGCAGA AAAGTTCACA CGTGAGGCGG CTAATCTAAA AGGGTCTATA 
AACGAGCTCC TCTTGAAGCT CAATTCCATG CAAGAAACTA AAGATATGCT AGAGTCCGGA 
AATGAAGAAC TCAAAGAACA GCTGAGAAAT TCCCAAAATC TGCGGAACAT GCTGGATGAG 
GAGAGTAAGA TGTGTATCTC CCTAAAAGAA AAATTAGTCA AACTGGAAGA TGCAAAAACT 
AGCCTTGAAC AGCAGTTGCG AGACAATAAA TCAGAAATAT ATCAAAGGCA CACGGAGCTT 
ACCAAGGAGG TGGAGCTGGG CCGCAACAGA ATTGGAGAAC TCACAAAGAA GTGTGAGGAG 
CTGTGCTCTG ATCTGGAAAA CTCAGACCAG ATACGACTCG ATCTGCAAGA AACTAAAGAG 
CAGTTGAAGA AAACGTTGGA AAATAACCTT GGATGGCAAC AGAAGGTTGA CGAAGTGACA 
AGAGAGTGCG AAAAGCTACG ATTTGATATG CAATCCAAAG AGGTGCAAAA TGAAAGTAAG 
GTTCAGGAAT TGATAAGCGA GTGCGAAGAG CTCCGGTCAA CTCTGGAAGC CAGTTTTCAG 
TCTGAGAAAG AAAGCATGGA CAGAACAATC TCAAGTCTTT TAGAAGACAA ACGCAACCTC 
GAGGAAAAGT TGTGCTCGGC TAACGATATT GTGGCTAAGC TTGAGACGGA AATAGCCGCG 
TTGCGGCCAA GAAAAAGTCT AGATCGGAAC CCAGTGCCGA GGAAATCGAT TACCTTTGAA 
TCCGAAATAC GGAAGAATCG ACGAATCAGC GTCCACGATG AGCGACGGCA GTCTTACTGG 
AACGACGTTC GGGAATTTGG CATCATGACA GATCCCGTTG ACAACAACTG CAACTGTGCG 
GAGCTGAATT CCAAGCTTCA GGACTGCCAG CGTGAGCTTT TCATTCGTGA AAGTCAAGTG 
ACGGCACTAA AGATGGAGCT GGACCATCAT CCGCTGAAGG ATGAGAATGC GCAACTAACG 
AAGCGAGTTA TTGAGGAGCA AGACAAGGCA AAGGTTGAGC AGAAACGGCT TAAAATGAAA 
CTTCAAGATC TTAATGCTAG GATTAATGAC CTCACTACGG CATCAGCCAA GGAACCAGAA 
TCTAACCAAA TGGCACAAGC AGCTAAACCT GCTACCGTAG CCGCTCAAAC TCAGACGGAA 
TCCGATCTTG AGACAATCCT TGAGAAAACG AACGTAAAGT ATCAGGAAGC GGTTCGCATG 
TTGCGCTACC GTTACCATCT CATCCAGGAA CTGAAAGAGA AACTTAGGCA AAACGAAAAC 
AGTGATACCT CGAATATTAC CTCACTCTCA GCTGGACAAA CCAGTGCATT GAAGGCACAG 
TGTGAGTCCC AAAAGAAGGA AATTTTGGCA ATCAAATATA AGTATGAAGC CGCCAAGCGA 
ATTTTGGCGA TTCGAAATGA TGATTTGGAT GCTCTGCGGG AAAAACTCGC GAAATATGAA 
ACAACTACAT CAGATAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cmet cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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