Myo28B1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo28B1
Entrez Gene ID	53515
Full Name	Myosin 28B1
Synonyms	28B,CG6976,DmVIIB,Dmel\CG6976,Myo18,Myo28B,VIIb 28B,myo 28B1,myo28B
General protein information	Preferred Names Myosin 28B1 Names CG6976 gene product from transcript CG6976-RB CG6976-PA CG6976-PB CG6976-PC CG6976-PD Myo28B1-PA Myo28B1-PB Myo28B1-PC Myo28B1-PD myosin 28B1 myosin VII myosin VII (28B) myosin VII 28B1-B4 myosin VIIB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28C1-28C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_164759.3	NP_723295.2	myosin 28B1, isoform C
NM_144373.4	NP_652630.2	myosin 28B1, isoform A
NM_164758.2	NP_723294.1	myosin 28B1, isoform B
NM_164760.4	NP_723296.2	myosin 28B1, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila myosin VIIB is a high duty ratio motor
Title: Myosin VIIB from Drosophila is a high duty ratio motor.

The following Myo28B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo28B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15332	NM_164759.3 Latest version!	Drosophila melanogaster myosin 28B1, transcript variant C (Myo28B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15334	NM_164760.4 Latest version!	Drosophila melanogaster myosin 28B1, transcript variant D (Myo28B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15331	NM_164758.2 Latest version!	Drosophila melanogaster myosin 28B1, transcript variant B (Myo28B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15333	NM_144373.4 Latest version!	Drosophila melanogaster myosin 28B1, transcript variant A (Myo28B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo28B1 ( NM_164759.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723295.2 Drosophila melanogaster myosin 28B1, transcript variant C (Myo28B1), mRNA.

Price & Availability↑

CloneID	OFa15332
Clone ID Related Accession (Same CDS sequence)	NM_164759.3
Accession Version	NM_164759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 28B1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_164759.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCG GCTATTCGCC CCGAAAAGGC GAGTATGTGT GGGTGAAGCC GCAAAATACT 
ACCAGCGAAT TCGCAGTGCC CTTTGGGGCT CGAATTGTGC GAACGGAGAA AACACAGACC 
CTAGTTTGCG ATGACCGGAA CAAGCAGTTC TGGGTTCCAG CTGGGGATGT CCTGAAGGCA 
ATGCATATCA CCTCCCAGGA GGATGTTGAG GATATGATCA CTTTGGGTGA CTTACAGGAG 
TACACTATAC TGAGGAACCT GCAGAACAGA TATGCCAAGC AATTAATATA TACCTACACA 
GGCTCCATGT TGGTGGCCAT CAATCCGTAC CAGATTCTGC CTATCTACAC GAATCGCGAG 
ATACAGCTCT ACAGAAATAA GTCCCTTGCT GAACTACCTC CTCACATTTT CGCCATTAGT 
GACAATGCCT TTCAGAGACT ACAAAGACTT AAGGAGAACC AGTGCGTGGT GATCAGCGGG 
GAGTCGGGTG CCGGGAAGAC GGAGAGCACC AAGCTGATCC TTCAGTATCT GGCCGCAATT 
AGTGGCAAAC ACTCGTGGAT CGAGCAGCAA ATCATAGAGG CCAATCCTAT TATGGAGGCC 
TTTGGCAATG CCAAGACTGT ACGGAATGAC AACTCTTCAA GGTTCGGAAA GTACATCGAG 
ATCAGATTCA CGCCACAGGG CGCTATTCAA GGAGCTAGAA TCCAGCAGTA TCTTCTGGAA 
AAATCCCGAA TTGTTTTCCA GAGTCGCGAT GAGCGCAACT ATCACATTTT CTACTGCATG 
TTGGCGGGAC TTTCAACTGC GGAGAGAGAG CGCCTAAAGC TACAGGAGCA ATCGCCAAGT 
CAGTATCATT ACTTGGCCCA AGGAGGATGC TTTACTTTGC CAGGAAGAGG TGATGCCAAG 
GATTTTGCCG ATATTCGAGC GGCTATGAAA GTGCTTTCAT TTAAGCCGGA GGAAGTGTGG 
AGCATACTTA GTTTACTGGC GGCTATTTTG CACTTGGGAA ACCTCAGATT CACAGCCACC 
GAGGTTGCCA ATTTGGCTAC GGCTGAGATA GACGATACAC CAAATCTTCA GAGGGTGGCT 
CAATTACTGG GAATACCCAT ATCTGCTCTG AATGCTGCTC TTACGCAAAG AACCATCTTT 
GTGCATGGTG AGCACGTGAC CACCAGTTTG TCCAAGGAAG CGGCTATTGA GGGTCGAGAT 
GCTTTTGTAA AATCGTTGTA CGATGGAATT TTTGTGCGCA TCGTGCGAAG AATCAATGAG 
ACTATAAACA AGCAAGTGGA TCAGCCCATG AATAGCATAG GTGTTCTAGA TATCTTCGGG 
TTCGAGAACT TCGACAATAA TAGCTTTGAA CAACTGTGCA TCAACTATGC CAACGAAAAT 
CTACAGCAAT TTTTCGTGGG ACATATCTTC AAGATGGAAC AGGATGAGTA CCAGAACGAG 
CATATCAACT GGCAGCACAT TGAGTTCCAG GATAATCAGC AAATCCTCGA TCTAATTGGC 
ATGAAACCCA TGAACTTGAT GTCCCTCATC GACGAAGAGT CCAAGTTTCC CAAGGGCACT 
GATCAAACTC TGCTCGAAAA GCTACATGTT CAGCATGGTA ATCGATCCAT TTACGTGAAA 
GGTAAAACCA CCCAAACGTC TCTATTCGGA ATCCGTCATT ATGCCGGCGT CGTGATGTAC 
AATCCATTGG GCTTTCTTGA AAAGAACAGG GATTCATTCA GCGGAGATCT TAGGACTCTG 
GTGCAGCGAT CAACAAATAA ATATCTGGTG GACATATTCC CCCATGAAAT GCCCATGGAT 
ACGGCCAAGA AGCAGCCCAC TTTGTGCGTG AAATTCAGAA ACTCTCTGGA CATGCTGATG 
CGCACTTTGT CGCAGGCACA TCCCTACTTT ATCCGTTGCA TCAAGCCCAA TGAATACAAG 
GAACCTAAGA ACTTTGACAA GGAGCTGTGC GTGCGGCAGT TGCGCTACTC AGGAATGATG 
GAGACGGCTC GCATTCGAAG GGCTGGCTAT CCGATCCGCC ACGCCTACCG TGCCTTCGTG 
GAGAGATATC GCCTCTTGGT GCCACCGGTG GGACCTTTGG AGCAATGTGA CTGCCGGAAG 
TTGGCGCGGC AGATCTGTGA AGTTGCCCTA CCTGCCGATT CTGATCGTCA GTACGGAAAA 
ACAAAGCTGT TCCTGCGGGA CGAGGACGAC GCTAGCCTGG AACTGCAGCG GTCCCAGCTG 
ATGCTCAAGA GTATCGTGAC AATTCAGCGA GGAATCAGAC GGGTCTTGTT CCGACGTTAC 
ATGAAGAGAT ACCGCGAGGC CATTATTACG GTTCAGAGAT ATTGGCGAGG TCGCCTGCAG 
CGTCGTAAGT ACCAGGTTAT GCGGCAGGGA TTCCATCGCC TGGGAGCCTG TATAGCTGCG 
CAGCAGTTGA CCACCAAGTT CACGATGGTC AGGTGTCGAA CCATAAAACT GCAGGCCTTG 
AGTCGCGGGT ATCTGGTACG CAAGGACTTC CAGAAAAAGT TGTTGGAGCG GCGAAAGCAG 
AACCAGCTGA AGAAGGAGGA GTTACTTAAG TTGGCCAAGA TGAAGGAAGC AGAGGAACTG 
CTAAGACTGC AGCAACTGAA GGAGCAGAAG GAGAGAGAAC AAAGAGAACA GCAGGAAAAA 
CGATTGCAGG AGGAGCAGAG GCTCAAGGCC GAAGCAGCAG CCAGAAATGC ATTGGCCATG 
GCTGCAGTGC AGCAGAAGAG GCGAACTAAG CCCGTCAAGC AAGAGGCTCC AAAGGCTCCA 
ACCCTGCAAG CACGCAACTC CCTGCCACCA CCACCCACCA CACTGATAGT AGCTGCCCCA 
CTACCCACTC GACCGGCCAG TGCCGTTACC AGAATTAATA CCATACCCGA GAGTCCTGGC 
ACCATTGATG TGGAGTCCTC CAAGCAAATG GTGGATGACG TGTTCCGCTT CCTAAACGAT 
GAACCCGATG TGAGTGGTCC TCTGGGGCCG AATGTCAAAG AGAAGTCTAT GATGTTCGAG 
CAGGCATTGC GTCTGCGTAG AGATGTGCCC ACCAAGCTCC TTTCCCGCCC GGTCAACTAT 
TACGAGGCGG CGCCTAGGAA GATTGTCAAC CAGACCCGCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723295.2
CDS	31..3135
Translation	MDAGYSPRKGEYVWVKPQNTTSEFAVPFGARIVRTEKTQTLVCDDRNKQFWVPAGDVLKAMHITSQEDVEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQILPIYTNREIQLYRNKSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYLAAISGKHSWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLSFKPEEVWSILSLLAAILHLGNLRFTATEVANLATAEIDDTPNLQRVAQLLGIPISALNAALTQRTIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQVDQPMNSIGVLDIFGFENFDNNSFEQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQDNQQILDLIGMKPMNLMSLIDEESKFPKGTDQTLLEKLHVQHGNRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRSTNKYLVDIFPHEMPMDTAKKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLLVPPVGPLEQCDCRKLARQICEVALPADSDRQYGKTKLFLRDEDDASLELQRSQLMLKSIVTIQRGIRRVLFRRYMKRYREAIITVQRYWRGRLQRRKYQVMRQGFHRLGACIAAQQLTTKFTMVRCRTIKLQALSRGYLVRKDFQKKLLERRKQNQLKKEELLKLAKMKEAEELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEAAARNALAMAAVQQKRRTKPVKQEAPKAPTLQARNSLPPPPTTLIVAAPLPTRPASAVTRINTIPESPGTIDVESSKQMVDDVFRFLNDEPDVSGPLGPNVKEKSMMFEQALRLRRDVPTKLLSRPVNYYEAAPRKIVNQTRL

Target ORF information:

RefSeq Version	NM_164759.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 28B1, transcript variant C (Myo28B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164759.3

ORF Insert Sequence:

ATGGACGCCG GCTATTCGCC CCGAAAAGGC GAGTATGTGT GGGTGAAGCC GCAAAATACT 
ACCAGCGAAT TCGCAGTGCC CTTTGGGGCT CGAATTGTGC GAACGGAGAA AACACAGACC 
CTAGTTTGCG ATGACCGGAA CAAGCAGTTC TGGGTTCCAG CTGGGGATGT CCTGAAGGCA 
ATGCATATCA CCTCCCAGGA GGATGTTGAG GATATGATCA CTTTGGGTGA CTTACAGGAG 
TACACTATAC TGAGGAACCT GCAGAACAGA TATGCCAAGC AATTAATATA TACCTACACA 
GGCTCCATGT TGGTGGCCAT CAATCCGTAC CAGATTCTGC CTATCTACAC GAATCGCGAG 
ATACAGCTCT ACAGAAATAA GTCCCTTGCT GAACTACCTC CTCACATTTT CGCCATTAGT 
GACAATGCCT TTCAGAGACT ACAAAGACTT AAGGAGAACC AGTGCGTGGT GATCAGCGGG 
GAGTCGGGTG CCGGGAAGAC GGAGAGCACC AAGCTGATCC TTCAGTATCT GGCCGCAATT 
AGTGGCAAAC ACTCGTGGAT CGAGCAGCAA ATCATAGAGG CCAATCCTAT TATGGAGGCC 
TTTGGCAATG CCAAGACTGT ACGGAATGAC AACTCTTCAA GGTTCGGAAA GTACATCGAG 
ATCAGATTCA CGCCACAGGG CGCTATTCAA GGAGCTAGAA TCCAGCAGTA TCTTCTGGAA 
AAATCCCGAA TTGTTTTCCA GAGTCGCGAT GAGCGCAACT ATCACATTTT CTACTGCATG 
TTGGCGGGAC TTTCAACTGC GGAGAGAGAG CGCCTAAAGC TACAGGAGCA ATCGCCAAGT 
CAGTATCATT ACTTGGCCCA AGGAGGATGC TTTACTTTGC CAGGAAGAGG TGATGCCAAG 
GATTTTGCCG ATATTCGAGC GGCTATGAAA GTGCTTTCAT TTAAGCCGGA GGAAGTGTGG 
AGCATACTTA GTTTACTGGC GGCTATTTTG CACTTGGGAA ACCTCAGATT CACAGCCACC 
GAGGTTGCCA ATTTGGCTAC GGCTGAGATA GACGATACAC CAAATCTTCA GAGGGTGGCT 
CAATTACTGG GAATACCCAT ATCTGCTCTG AATGCTGCTC TTACGCAAAG AACCATCTTT 
GTGCATGGTG AGCACGTGAC CACCAGTTTG TCCAAGGAAG CGGCTATTGA GGGTCGAGAT 
GCTTTTGTAA AATCGTTGTA CGATGGAATT TTTGTGCGCA TCGTGCGAAG AATCAATGAG 
ACTATAAACA AGCAAGTGGA TCAGCCCATG AATAGCATAG GTGTTCTAGA TATCTTCGGG 
TTCGAGAACT TCGACAATAA TAGCTTTGAA CAACTGTGCA TCAACTATGC CAACGAAAAT 
CTACAGCAAT TTTTCGTGGG ACATATCTTC AAGATGGAAC AGGATGAGTA CCAGAACGAG 
CATATCAACT GGCAGCACAT TGAGTTCCAG GATAATCAGC AAATCCTCGA TCTAATTGGC 
ATGAAACCCA TGAACTTGAT GTCCCTCATC GACGAAGAGT CCAAGTTTCC CAAGGGCACT 
GATCAAACTC TGCTCGAAAA GCTACATGTT CAGCATGGTA ATCGATCCAT TTACGTGAAA 
GGTAAAACCA CCCAAACGTC TCTATTCGGA ATCCGTCATT ATGCCGGCGT CGTGATGTAC 
AATCCATTGG GCTTTCTTGA AAAGAACAGG GATTCATTCA GCGGAGATCT TAGGACTCTG 
GTGCAGCGAT CAACAAATAA ATATCTGGTG GACATATTCC CCCATGAAAT GCCCATGGAT 
ACGGCCAAGA AGCAGCCCAC TTTGTGCGTG AAATTCAGAA ACTCTCTGGA CATGCTGATG 
CGCACTTTGT CGCAGGCACA TCCCTACTTT ATCCGTTGCA TCAAGCCCAA TGAATACAAG 
GAACCTAAGA ACTTTGACAA GGAGCTGTGC GTGCGGCAGT TGCGCTACTC AGGAATGATG 
GAGACGGCTC GCATTCGAAG GGCTGGCTAT CCGATCCGCC ACGCCTACCG TGCCTTCGTG 
GAGAGATATC GCCTCTTGGT GCCACCGGTG GGACCTTTGG AGCAATGTGA CTGCCGGAAG 
TTGGCGCGGC AGATCTGTGA AGTTGCCCTA CCTGCCGATT CTGATCGTCA GTACGGAAAA 
ACAAAGCTGT TCCTGCGGGA CGAGGACGAC GCTAGCCTGG AACTGCAGCG GTCCCAGCTG 
ATGCTCAAGA GTATCGTGAC AATTCAGCGA GGAATCAGAC GGGTCTTGTT CCGACGTTAC 
ATGAAGAGAT ACCGCGAGGC CATTATTACG GTTCAGAGAT ATTGGCGAGG TCGCCTGCAG 
CGTCGTAAGT ACCAGGTTAT GCGGCAGGGA TTCCATCGCC TGGGAGCCTG TATAGCTGCG 
CAGCAGTTGA CCACCAAGTT CACGATGGTC AGGTGTCGAA CCATAAAACT GCAGGCCTTG 
AGTCGCGGGT ATCTGGTACG CAAGGACTTC CAGAAAAAGT TGTTGGAGCG GCGAAAGCAG 
AACCAGCTGA AGAAGGAGGA GTTACTTAAG TTGGCCAAGA TGAAGGAAGC AGAGGAACTG 
CTAAGACTGC AGCAACTGAA GGAGCAGAAG GAGAGAGAAC AAAGAGAACA GCAGGAAAAA 
CGATTGCAGG AGGAGCAGAG GCTCAAGGCC GAAGCAGCAG CCAGAAATGC ATTGGCCATG 
GCTGCAGTGC AGCAGAAGAG GCGAACTAAG CCCGTCAAGC AAGAGGCTCC AAAGGCTCCA 
ACCCTGCAAG CACGCAACTC CCTGCCACCA CCACCCACCA CACTGATAGT AGCTGCCCCA 
CTACCCACTC GACCGGCCAG TGCCGTTACC AGAATTAATA CCATACCCGA GAGTCCTGGC 
ACCATTGATG TGGAGTCCTC CAAGCAAATG GTGGATGACG TGTTCCGCTT CCTAAACGAT 
GAACCCGATG TGAGTGGTCC TCTGGGGCCG AATGTCAAAG AGAAGTCTAT GATGTTCGAG 
CAGGCATTGC GTCTGCGTAG AGATGTGCCC ACCAAGCTCC TTTCCCGCCC GGTCAACTAT 
TACGAGGCGG CGCCTAGGAA GATTGTCAAC CAGACCCGCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo28B1 ( NM_164760.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723296.2 Drosophila melanogaster myosin 28B1, transcript variant D (Myo28B1), mRNA.

Price & Availability↑

CloneID	OFa15334
Clone ID Related Accession (Same CDS sequence)	NM_164760.4
Accession Version	NM_164760.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 28B1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164760.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAAT TTGTGCGGCA ACATGGCGAG TATGTGTGGG TGAAGCCGCA AAATACTACC 
AGCGAATTCG CAGTGCCCTT TGGGGCTCGA ATTGTGCGAA CGGAGAAAAC ACAGACCCTA 
GTTTGCGATG ACCGGAACAA GCAGTTCTGG GTTCCAGCTG GGGATGTCCT GAAGGCAATG 
CATATCACCT CCCAGGAGGA TGTTGAGGAT ATGATCACTT TGGGTGACTT ACAGGAGTAC 
ACTATACTGA GGAACCTGCA GAACAGATAT GCCAAGCAAT TAATATATAC CTACACAGGC 
TCCATGTTGG TGGCCATCAA TCCGTACCAG ATTCTGCCTA TCTACACGAA TCGCGAGATA 
CAGCTCTACA GAAATAAGTC CCTTGCTGAA CTACCTCCTC ACATTTTCGC CATTAGTGAC 
AATGCCTTTC AGAGACTACA AAGACTTAAG GAGAACCAGT GCGTGGTGAT CAGCGGGGAG 
TCGGGTGCCG GGAAGACGGA GAGCACCAAG CTGATCCTTC AGTATCTGGC CGCAATTAGT 
GGCAAACACT CGTGGATCGA GCAGCAAATC ATAGAGGCCA ATCCTATTAT GGAGGCCTTT 
GGCAATGCCA AGACTGTACG GAATGACAAC TCTTCAAGGT TCGGAAAGTA CATCGAGATC 
AGATTCACGC CACAGGGCGC TATTCAAGGA GCTAGAATCC AGCAGTATCT TCTGGAAAAA 
TCCCGAATTG TTTTCCAGAG TCGCGATGAG CGCAACTATC ACATTTTCTA CTGCATGTTG 
GCGGGACTTT CAACTGCGGA GAGAGAGCGC CTAAAGCTAC AGGAGCAATC GCCAAGTCAG 
TATCATTACT TGGCCCAAGG AGGATGCTTT ACTTTGCCAG GAAGAGGTGA TGCCAAGGAT 
TTTGCCGATA TTCGAGCGGC TATGAAAGTG CTTTCATTTA AGCCGGAGGA AGTGTGGAGC 
ATACTTAGTT TACTGGCGGC TATTTTGCAC TTGGGAAACC TCAGATTCAC AGCCACCGAG 
GTTGCCAATT TGGCTACGGC TGAGATAGAC GATACACCAA ATCTTCAGAG GGTGGCTCAA 
TTACTGGGAA TACCCATATC TGCTCTGAAT GCTGCTCTTA CGCAAAGAAC CATCTTTGTG 
CATGGTGAGC ACGTGACCAC CAGTTTGTCC AAGGAAGCGG CTATTGAGGG TCGAGATGCT 
TTTGTAAAAT CGTTGTACGA TGGAATTTTT GTGCGCATCG TGCGAAGAAT CAATGAGACT 
ATAAACAAGC AAGTGGATCA GCCCATGAAT AGCATAGGTG TTCTAGATAT CTTCGGGTTC 
GAGAACTTCG ACAATAATAG CTTTGAACAA CTGTGCATCA ACTATGCCAA CGAAAATCTA 
CAGCAATTTT TCGTGGGACA TATCTTCAAG ATGGAACAGG ATGAGTACCA GAACGAGCAT 
ATCAACTGGC AGCACATTGA GTTCCAGGAT AATCAGCAAA TCCTCGATCT AATTGGCATG 
AAACCCATGA ACTTGATGTC CCTCATCGAC GAAGAGTCCA AGTTTCCCAA GGGCACTGAT 
CAAACTCTGC TCGAAAAGCT ACATGTTCAG CATGGTAATC GATCCATTTA CGTGAAAGGT 
AAAACCACCC AAACGTCTCT ATTCGGAATC CGTCATTATG CCGGCGTCGT GATGTACAAT 
CCATTGGGCT TTCTTGAAAA GAACAGGGAT TCATTCAGCG GAGATCTTAG GACTCTGGTG 
CAGCGATCAA CAAATAAATA TCTGGTGGAC ATATTCCCCC ATGAAATGCC CATGGATACG 
GCCAAGAAGC AGCCCACTTT GTGCGTGAAA TTCAGAAACT CTCTGGACAT GCTGATGCGC 
ACTTTGTCGC AGGCACATCC CTACTTTATC CGTTGCATCA AGCCCAATGA ATACAAGGAA 
CCTAAGAACT TTGACAAGGA GCTGTGCGTG CGGCAGTTGC GCTACTCAGG AATGATGGAG 
ACGGCTCGCA TTCGAAGGGC TGGCTATCCG ATCCGCCACG CCTACCGTGC CTTCGTGGAG 
AGATATCGCC TCTTGGTGCC ACCGGTGGGA CCTTTGGAGC AATGTGACTG CCGGAAGTTG 
GCGCGGCAGA TCTGTGAAGT TGCCCTACCT GCCGATTCTG ATCGTCAGTA CGGAAAAACA 
AAGCTGTTCC TGCGGGACGA GGACGACGCT AGCCTGGAAC TGCAGCGGTC CCAGCTGATG 
CTCAAGAGTA TCGTGACAAT TCAGCGAGGA ATCAGACGGG TCTTGTTCCG ACGTTACATG 
AAGAGATACC GCGAGGCCAT TATTACGGTT CAGAGATATT GGCGAGGTCG CCTGCAGCGT 
CGTAAGTACC AGGTTATGCG GCAGGGATTC CATCGCCTGG GAGCCTGTAT AGCTGCGCAG 
CAGTTGACCA CCAAGTTCAC GATGGTCAGG TGTCGAACCA TAAAACTGCA GGCCTTGAGT 
CGCGGGTATC TGGTACGCAA GGACTTCCAG AAAAAGTTGT TGGAGCGGCG AAAGCAGAAC 
CAGCTGAAGA AGGAGGAGTT ACTTAAGTTG GCCAAGATGA AGGAAGCAGA GGAACTGCTA 
AGACTGCAGC AACTGAAGGA GCAGAAGGAG AGAGAACAAA GAGAACAGCA GGAAAAACGA 
TTGCAGGAGG AGCAGAGGCT CAAGGCCGAA GCAGCAGCCA GAAATGCATT GGCCATGGCT 
GCAGTGCAGC AGAAGAGGCG AACTAAGCCC GTCAAGCAAG AGGCTCCAAA GGCTCCAACC 
CTGCAAGCAC GCAACTCCCT GCCACCACCA CCCACCACAC TGATAGTAGC TGCCCCACTA 
CCCACTCGAC CGGCCAGTGC CGTTACCAGA ATTAATACCA TACCCGAGAG TCCTGGCACC 
ATTGATGTGG AGTCCTCCAA GCAAATGGTG GATGACGTGT TCCGCTTCCT AAACGATGAA 
CCCGATGTGA GTGGTCCTCT GGGGCCGAAT GTCAAAGAGA AGTCTATGAT GTTCGAGCAG 
GCATTGCGTC TGCGTAGAGA TGTGCCCACC AAGCTCCTTT CCCGCCCGGT CAACTATTAC 
GAGGCGGCGC CTAGGAAGAT TGTCAACCAG ACCCGCCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723296.2
CDS	179..3280
Translation	MSEFVRQHGEYVWVKPQNTTSEFAVPFGARIVRTEKTQTLVCDDRNKQFWVPAGDVLKAMHITSQEDVEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQILPIYTNREIQLYRNKSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYLAAISGKHSWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLSFKPEEVWSILSLLAAILHLGNLRFTATEVANLATAEIDDTPNLQRVAQLLGIPISALNAALTQRTIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQVDQPMNSIGVLDIFGFENFDNNSFEQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQDNQQILDLIGMKPMNLMSLIDEESKFPKGTDQTLLEKLHVQHGNRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRSTNKYLVDIFPHEMPMDTAKKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLLVPPVGPLEQCDCRKLARQICEVALPADSDRQYGKTKLFLRDEDDASLELQRSQLMLKSIVTIQRGIRRVLFRRYMKRYREAIITVQRYWRGRLQRRKYQVMRQGFHRLGACIAAQQLTTKFTMVRCRTIKLQALSRGYLVRKDFQKKLLERRKQNQLKKEELLKLAKMKEAEELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEAAARNALAMAAVQQKRRTKPVKQEAPKAPTLQARNSLPPPPTTLIVAAPLPTRPASAVTRINTIPESPGTIDVESSKQMVDDVFRFLNDEPDVSGPLGPNVKEKSMMFEQALRLRRDVPTKLLSRPVNYYEAAPRKIVNQTRL

Target ORF information:

RefSeq Version	NM_164760.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 28B1, transcript variant D (Myo28B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164760.4

ORF Insert Sequence:

ATGTCCGAAT TTGTGCGGCA ACATGGCGAG TATGTGTGGG TGAAGCCGCA AAATACTACC 
AGCGAATTCG CAGTGCCCTT TGGGGCTCGA ATTGTGCGAA CGGAGAAAAC ACAGACCCTA 
GTTTGCGATG ACCGGAACAA GCAGTTCTGG GTTCCAGCTG GGGATGTCCT GAAGGCAATG 
CATATCACCT CCCAGGAGGA TGTTGAGGAT ATGATCACTT TGGGTGACTT ACAGGAGTAC 
ACTATACTGA GGAACCTGCA GAACAGATAT GCCAAGCAAT TAATATATAC CTACACAGGC 
TCCATGTTGG TGGCCATCAA TCCGTACCAG ATTCTGCCTA TCTACACGAA TCGCGAGATA 
CAGCTCTACA GAAATAAGTC CCTTGCTGAA CTACCTCCTC ACATTTTCGC CATTAGTGAC 
AATGCCTTTC AGAGACTACA AAGACTTAAG GAGAACCAGT GCGTGGTGAT CAGCGGGGAG 
TCGGGTGCCG GGAAGACGGA GAGCACCAAG CTGATCCTTC AGTATCTGGC CGCAATTAGT 
GGCAAACACT CGTGGATCGA GCAGCAAATC ATAGAGGCCA ATCCTATTAT GGAGGCCTTT 
GGCAATGCCA AGACTGTACG GAATGACAAC TCTTCAAGGT TCGGAAAGTA CATCGAGATC 
AGATTCACGC CACAGGGCGC TATTCAAGGA GCTAGAATCC AGCAGTATCT TCTGGAAAAA 
TCCCGAATTG TTTTCCAGAG TCGCGATGAG CGCAACTATC ACATTTTCTA CTGCATGTTG 
GCGGGACTTT CAACTGCGGA GAGAGAGCGC CTAAAGCTAC AGGAGCAATC GCCAAGTCAG 
TATCATTACT TGGCCCAAGG AGGATGCTTT ACTTTGCCAG GAAGAGGTGA TGCCAAGGAT 
TTTGCCGATA TTCGAGCGGC TATGAAAGTG CTTTCATTTA AGCCGGAGGA AGTGTGGAGC 
ATACTTAGTT TACTGGCGGC TATTTTGCAC TTGGGAAACC TCAGATTCAC AGCCACCGAG 
GTTGCCAATT TGGCTACGGC TGAGATAGAC GATACACCAA ATCTTCAGAG GGTGGCTCAA 
TTACTGGGAA TACCCATATC TGCTCTGAAT GCTGCTCTTA CGCAAAGAAC CATCTTTGTG 
CATGGTGAGC ACGTGACCAC CAGTTTGTCC AAGGAAGCGG CTATTGAGGG TCGAGATGCT 
TTTGTAAAAT CGTTGTACGA TGGAATTTTT GTGCGCATCG TGCGAAGAAT CAATGAGACT 
ATAAACAAGC AAGTGGATCA GCCCATGAAT AGCATAGGTG TTCTAGATAT CTTCGGGTTC 
GAGAACTTCG ACAATAATAG CTTTGAACAA CTGTGCATCA ACTATGCCAA CGAAAATCTA 
CAGCAATTTT TCGTGGGACA TATCTTCAAG ATGGAACAGG ATGAGTACCA GAACGAGCAT 
ATCAACTGGC AGCACATTGA GTTCCAGGAT AATCAGCAAA TCCTCGATCT AATTGGCATG 
AAACCCATGA ACTTGATGTC CCTCATCGAC GAAGAGTCCA AGTTTCCCAA GGGCACTGAT 
CAAACTCTGC TCGAAAAGCT ACATGTTCAG CATGGTAATC GATCCATTTA CGTGAAAGGT 
AAAACCACCC AAACGTCTCT ATTCGGAATC CGTCATTATG CCGGCGTCGT GATGTACAAT 
CCATTGGGCT TTCTTGAAAA GAACAGGGAT TCATTCAGCG GAGATCTTAG GACTCTGGTG 
CAGCGATCAA CAAATAAATA TCTGGTGGAC ATATTCCCCC ATGAAATGCC CATGGATACG 
GCCAAGAAGC AGCCCACTTT GTGCGTGAAA TTCAGAAACT CTCTGGACAT GCTGATGCGC 
ACTTTGTCGC AGGCACATCC CTACTTTATC CGTTGCATCA AGCCCAATGA ATACAAGGAA 
CCTAAGAACT TTGACAAGGA GCTGTGCGTG CGGCAGTTGC GCTACTCAGG AATGATGGAG 
ACGGCTCGCA TTCGAAGGGC TGGCTATCCG ATCCGCCACG CCTACCGTGC CTTCGTGGAG 
AGATATCGCC TCTTGGTGCC ACCGGTGGGA CCTTTGGAGC AATGTGACTG CCGGAAGTTG 
GCGCGGCAGA TCTGTGAAGT TGCCCTACCT GCCGATTCTG ATCGTCAGTA CGGAAAAACA 
AAGCTGTTCC TGCGGGACGA GGACGACGCT AGCCTGGAAC TGCAGCGGTC CCAGCTGATG 
CTCAAGAGTA TCGTGACAAT TCAGCGAGGA ATCAGACGGG TCTTGTTCCG ACGTTACATG 
AAGAGATACC GCGAGGCCAT TATTACGGTT CAGAGATATT GGCGAGGTCG CCTGCAGCGT 
CGTAAGTACC AGGTTATGCG GCAGGGATTC CATCGCCTGG GAGCCTGTAT AGCTGCGCAG 
CAGTTGACCA CCAAGTTCAC GATGGTCAGG TGTCGAACCA TAAAACTGCA GGCCTTGAGT 
CGCGGGTATC TGGTACGCAA GGACTTCCAG AAAAAGTTGT TGGAGCGGCG AAAGCAGAAC 
CAGCTGAAGA AGGAGGAGTT ACTTAAGTTG GCCAAGATGA AGGAAGCAGA GGAACTGCTA 
AGACTGCAGC AACTGAAGGA GCAGAAGGAG AGAGAACAAA GAGAACAGCA GGAAAAACGA 
TTGCAGGAGG AGCAGAGGCT CAAGGCCGAA GCAGCAGCCA GAAATGCATT GGCCATGGCT 
GCAGTGCAGC AGAAGAGGCG AACTAAGCCC GTCAAGCAAG AGGCTCCAAA GGCTCCAACC 
CTGCAAGCAC GCAACTCCCT GCCACCACCA CCCACCACAC TGATAGTAGC TGCCCCACTA 
CCCACTCGAC CGGCCAGTGC CGTTACCAGA ATTAATACCA TACCCGAGAG TCCTGGCACC 
ATTGATGTGG AGTCCTCCAA GCAAATGGTG GATGACGTGT TCCGCTTCCT AAACGATGAA 
CCCGATGTGA GTGGTCCTCT GGGGCCGAAT GTCAAAGAGA AGTCTATGAT GTTCGAGCAG 
GCATTGCGTC TGCGTAGAGA TGTGCCCACC AAGCTCCTTT CCCGCCCGGT CAACTATTAC 
GAGGCGGCGC CTAGGAAGAT TGTCAACCAG ACCCGCCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo28B1 ( NM_164758.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723294.1 Drosophila melanogaster myosin 28B1, transcript variant B (Myo28B1), mRNA.

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CloneID	OFa15331
Clone ID Related Accession (Same CDS sequence)	NM_164758.2
Accession Version	NM_164758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 28B1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_164758.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCG GCTATTCGCC CCGAAAAGGC GAGTATGTGT GGGTGAAGCC GCAAAATACT 
ACCAGCGAAT TCGCAGTGCC CTTTGGGGCT CGAATTGTGC GAACGGAGAA AACACAGACC 
CTAGTTTGCG ATGACCGGAA CAAGCAGTTC TGGGTTCCAG CTGGGGATGT CCTGAAGGCA 
ATGCATATCA CCTCCCAGGA GGATGTTGAG GATATGATCA CTTTGGGTGA CTTACAGGAG 
TACACTATAC TGAGGAACCT GCAGAACAGA TATGCCAAGC AATTAATATA TACCTACACA 
GGCTCCATGT TGGTGGCCAT CAATCCGTAC CAGATTCTGC CTATCTACAC GAATCGCGAG 
ATACAGCTCT ACAGAAATAA GTCCCTTGCT GAACTACCTC CTCACATTTT CGCCATTAGT 
GACAATGCCT TTCAGAGACT ACAAAGACTT AAGGAGAACC AGTGCGTGGT GATCAGCGGG 
GAGTCGGGTG CCGGGAAGAC GGAGAGCACC AAGCTGATCC TTCAGTATCT GGCCGCAATT 
AGTGGCAAAC ACTCGTGGAT CGAGCAGCAA ATCATAGAGG CCAATCCTAT TATGGAGGCC 
TTTGGCAATG CCAAGACTGT ACGGAATGAC AACTCTTCAA GGTTCGGAAA GTACATCGAG 
ATCAGATTCA CGCCACAGGG CGCTATTCAA GGAGCTAGAA TCCAGCAGTA TCTTCTGGAA 
AAATCCCGAA TTGTTTTCCA GAGTCGCGAT GAGCGCAACT ATCACATTTT CTACTGCATG 
TTGGCGGGAC TTTCAACTGC GGAGAGAGAG CGCCTAAAGC TACAGGAGCA ATCGCCAAGT 
CAGTATCATT ACTTGGCCCA AGGAGGATGC TTTACTTTGC CAGGAAGAGG TGATGCCAAG 
GATTTTGCCG ATATTCGAGC GGCTATGAAA GTGCTTTCAT TTAAGCCGGA GGAAGTGTGG 
AGCATACTTA GTTTACTGGC GGCTATTTTG CACTTGGGAA ACCTCAGATT CACAGCCACC 
GAGGTTGCCA ATTTGGCTAC GGCTGAGATA GACGATACAC CAAATCTTCA GAGGGTGGCT 
CAATTACTGG GAATACCCAT ATCTGCTCTG AATGCTGCTC TTACGCAAAG AACCATCTTT 
GTGCATGGTG AGCACGTGAC CACCAGTTTG TCCAAGGAAG CGGCTATTGA GGGTCGAGAT 
GCTTTTGTAA AATCGTTGTA CGATGGAATT TTTGTGCGCA TCGTGCGAAG AATCAATGAG 
ACTATAAACA AGCAAGTGGA TCAGCCCATG AATAGCATAG GTGTTCTAGA TATCTTCGGG 
TTCGAGAACT TCGACAATAA TAGCTTTGAA CAACTGTGCA TCAACTATGC CAACGAAAAT 
CTACAGCAAT TTTTCGTGGG ACATATCTTC AAGATGGAAC AGGATGAGTA CCAGAACGAG 
CATATCAACT GGCAGCACAT TGAGTTCCAG GATAATCAGC AAATCCTCGA TCTAATTGGC 
ATGAAACCCA TGAACTTGAT GTCCCTCATC GACGAAGAGT CCAAGTTTCC CAAGGGCACT 
GATCAAACTC TGCTCGAAAA GCTACATGTT CAGCATGGTA ATCGATCCAT TTACGTGAAA 
GGTAAAACCA CCCAAACGTC TCTATTCGGA ATCCGTCATT ATGCCGGCGT CGTGATGTAC 
AATCCATTGG GCTTTCTTGA AAAGAACAGG GATTCATTCA GCGGAGATCT TAGGACTCTG 
GTGCAGCGAT CAACAAATAA ATATCTGGTG GACATATTCC CCCATGAAAT GCCCATGGAT 
ACGGCCAAGA AGCAGCCCAC TTTGTGCGTG AAATTCAGAA ACTCTCTGGA CATGCTGATG 
CGCACTTTGT CGCAGGCACA TCCCTACTTT ATCCGTTGCA TCAAGCCCAA TGAATACAAG 
GAACCTAAGA ACTTTGACAA GGAGCTGTGC GTGCGGCAGT TGCGCTACTC AGGAATGATG 
GAGACGGCTC GCATTCGAAG GGCTGGCTAT CCGATCCGCC ACGCCTACCG TGCCTTCGTG 
GAGAGATATC GCCTCTTGGT GCCACCGGTG GGACCTTTGG AGCAATGTGA CTGCCGGAAG 
TTGGCGCGGC AGATCTGTGA AGTTGCCCTA CCTGCCGATT CTGATCGTCA GTACGGAAAA 
ACAAAGCTGT TCCTGCGGGA CGAGGACGAC GCTAGCCTGG AACTGCAGCG GTCCCAGCTG 
ATGCTCAAGA GTATCGTGAC AATTCAGCGA GGAATCAGAC GGGTCTTGTT CCGACGTTAC 
ATGAAGAGAT ACCGCGAGGC CATTATTACG GTTCAGAGAT ATTGGCGAGG TCGCCTGCAG 
CGTCGTAAGT ACCAGGTTAT GCGGCAGGGA TTCCATCGCC TGGGAGCCTG TATAGCTGCG 
CAGCAGTTGA CCACCAAGTT CACGATGGTC AGGTGTCGAA CCATAAAACT GCAGGCCTTG 
AGTCGCGGGT ATCTGGTACG CAAGGACTTC CAGAAAAAGT TGTTGGAGCG GCGAAAGCAG 
AACCAGCTGA AGAAGGAGGA GTTACTTAAG TTGGCCAAGA TGAAGGAAGC AGAGGAACTG 
CTAAGACTGC AGCAACTGAA GGAGCAGAAG GAGAGAGAAC AAAGAGAACA GCAGGAAAAA 
CGATTGCAGG AGGAGCAGAG GCTCAAGGCC GAAGCAGCAG CCAGAAATGC ATTGGCCATG 
GCTGCAGTGC AGCAGAAGAG GCGAACTAAG CCCGTCAAGC AAGAGGCTCC AAAGGCTCCA 
ACCCTGCAAG CACGCAACTC CCTGCCACCA CCACCCACCA CACTGATAGT AGCTGCCCCA 
CTACCCACTC GACCGGCCAG TGCCGTTACC AGAATTAATA CCATACCCGA GAGTCCTGGC 
ACCATTGATG TGGAGTCCTC CAAGCAAATG GTGGATGACG TGTTCCGCTT CCTAAACGAT 
GAACCCGATG CAGCCTTGAG GAAACTTAAC AACATCTCTT CTGGAGATAC AATACGTCTA 
CCTAAATCGG TGCCAAATAA CATCGACACC TCCGATTTCA GTTATTTGAA ATATGCGGCT 
ACTTACTTCG GCGGTGGCGC CACTGCACAG CACGAAAGAA AGCCTCTGAA GAAATCTTTG 
TTAAAACATG AACATCCCAT TGATGAGATG GCCTCTAAGG CCATTTGGTT GACTATACTC 
CGCTTTATGG GCGACTTGCC GGATGTTGTT TCCTCGCCTA CTCTTCACGT ATTCGATAAT 
GAAAACTTGA TGAGTGATTT GGCAAGCCTC TTGAACACTT CTGATTCCTA TAAGCCTCGC 
TTATTTGTGC GCCAATCCCA AAGACGTATC CCAAAGCCTT TGGCTAGTGG TGAGAAGGAA 
GCCCAGGAAT TCTATCAACA CTGGCTCAAC GTTCCCACAA GTCACCTGGA GAAAATTCAC 
TTCATCATAG GTCATGGTAT AATAAAGAAT AGCTTACGAG ACGAAATCTT GGCCCAGATC 
TGCAAGCAGC TATATCTTAA CCCAAGTCGG AGCTCCTACT CCCGCGGATG GTTGCTCCTT 
TCGCTATGCC TCAGCTGCTT TCCACCCAGC AAGGAGTTTG AGCCACACTT GCGCAGTTTC 
ATGAAGCAGG GAACTGCCCA ACTGCAGGCC ACTCCATCCC TGCAGCGATT GGAGCGAACC 
CTGGTCAATG GACCACGCTG TCAGCCACCT TCGTTATTTG AGCTCCACGC AATCCGCGGT 
CGCCACCCGC TTAGGTTGGA CATTCACCTG ATGGATGGGC AGCAGAGAAG ATTGCAGGTG 
GATGCGGCCA GTACAGCTCG AGAGGCAGTC AATCAACTTT GTCAGGGTAT GGGGCTAACC 
GACACCTTTG GCTTTGGATT GGTGATGTCA TTGAATGGGA AGCTAATGCC GCTGGGAGCA 
GGTCAGGAGC ATGTCCTGGA TGCCATTTCG GAGTGCGAGC AACGTCAGCT GGATGCACCT 
TGGAAACTAT ACATCCGAAA GGAAATGTTT GCCACTTGGT ATGATCCCTC GATGGATCCC 
AAGGCCACTC AACTTATATA CAAGCAAATT CTAAACGGGT TGAAGTGCGG AGAGTATCGA 
TGTCGCTCCG AGAAGGACAT AGCAATGGTG TGTGCTCTGG CCTGCTTTGT AGAATATGGA 
CCTGGGGAGA TTCTTCGATT GAAGCCATCG GAGATCACTG CATTTGTCCC AAGTGATCTT 
CTAGCTCCCG GCGAGCGAGC CATTGAGAAC TGGAGTCGCC TAATAGCTGC CACCTACGAG 
AAGAGCTCTT ATGTCAAAGA AGAACAGAAT GACCTGCTAT TGGAGGCGCA GAAGAGAGCC 
AAGGAGGATA TATGTCTGTT TGCACATCTC TCCTGGCCTA TGAGGCACTC CCGACTGTTT 
GAAGTGGTTC GCAAAGAGGG ACCAAAACTC CAAAGCGATG AACTTATGCT GGGCATTAAT 
TCCGCGGGCC TTTTCCTTAT CGACGAAACC GAACAGGTGC TGGCATCCTG TTGTTTTAGC 
GAGGTGCTGA AAGTGCACGT GGAATCAGAT GATAAACTGC ATGTTATGAC CTTCCAACAC 
GTCAATTTTG TGCTGCAGTG CAGCTCTGCT CAGGATGCCA ACGAGGTGAT AAACTATATG 
CTGGATAATC TCCGACAGCG TTCAAGCTAT GGCGTAGCTC TTGATCCGGT CGTAGAGGGG 
GATCTAGAGG ATTGTCTAGT GCTAAACCCC GGTGACCTAA TCGAATTCGA GGCAGGTGTA 
ACAGGTGCCC AGTTAATGGC TGGTAATGCA CAAGACTGCT ACAGGGGATG TGTCAATGGC 
CAGTGGGGTC AGTTTTTAGC TGGAAATGTT CGCGTTTTGG CCACGCTCAC CAAGCCGAGT 
GAAAAACTTC AAGATATCCT CCGAGAAGGC CGATTTCAAG AACCCCCGAA ACCAACTCCA 
AGAGCCAATT ACTCTCGTCG TCGTCAACAC AATATCTCTC AGCTGGCTGA GAGCCACTTT 
AGGGAGCCAT TGGACTCCGA TAAGGCACCA CTATCAAAGT TTTCACCTGA GCCTCTGAAA 
GCCCCGCTGC TCAAAGCTGT TGTTAAGGTA CCGCCACTTT TCCAGCAGGC ATTGGTTATG 
CATCATCATA TTCTTAAGTA CATGGGTGAC ATAGCAAGGA GCAATCTACC GGTCAACACG 
GACCTCATTT TCCAGCCAGC ACTGCAGCAT CCGCTGTTGT GTGACGAACT CTACTGCCAG 
CTGATGAAGC AACTGAGCGA TAATCCCTCC AGTGAAAGCG AGAAGCGGGG CTGGGACCTG 
CTTTACTTGG CCACGGGCTT GGTGGCTCCC AGTGTCTTGG TCATGAGGGA GCTGATCATA 
CTACTGCGCA TGCGTGCAGA CGCCCTGGCC GATGCTTGCC TGAAGCGACT GAAGCGATCC 
TTGGCACAGG GCCAGCGAAA AAAGGCACCA CATCTCATAG AGGTCGAGGG AATTCAACAG 
CGATGCCTGC ATATCTACCA CAAGATATAT TTCCCCGACG ATACGGTTGA GGCTTTCGAG 
ATTGAATCAC ACACCCGGGG AGCAGAGCTC ATAGCGGACA TAGCCCAGAG ATTGGAGCTC 
AAATCACCTG TGGGTTATAG TATTTTCTTG AAGACAGGCG ACAGGGTCTA TGCTATGCCG 
GAAGAGGAGT TCGTTTTTGA TTTCATCACC CAACTCATCT ACTGGCTAAG ACAGCAGAGG 
ACCATACGCT CCATATCCGA TGGCCAATAC CAACTGCACT TCATGAGAAA GTTATGGTTA 
AACAACCATC CAGGCGAAGA CCTCAATGGA GATATGATCT TTAGCTATCC CCAAGAGCTG 
CACAAATACT TGAAGGGCTA CTATCCCATC GATTGCGAGC AGGCCTCGCG TTTGGCAATT 
CTTGTGTACA GTGCAGATCA TGACGTTAGC TTGCAGCGGC TGCCCGAAGT GCTGACACGT 
TTAATTCCTG AAGACCTAAT ACCGCTTCAA ACGGTGGCCG AATGGAGGCA GCAGATCCTG 
CCCAAGGTCC ACCGCGATCA TTTAACGGAA GATCACGCCA AGATTTTGTT CCTTCAAGAG 
CTGTCCCATT TCGCTTGCTT CGGTTCCACA TTCTTTGTGG TGAAGCAACA AAATGACGAT 
GCCCTTCCCG AAACTCTTTT AATCGCAATT AACAGCACAG GTTTCCATAT GCTGGATCCG 
ACAACTAAGG AGATCCTTCG CAGCTATGAA TACTCCCAGT TGGGTATTTG GAGCTCGGGT 
AAGAACCACT TCCACATTCG TTTTGGTAAC ATGATCGGAG CATCGAAGCT GCTGTGCAGC 
ACCACCCAAG GATACAAAAT GGACGATTTG CTGGCCTCTT ATGTTAAGTA TTTTAATGAA 
CACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723294.1
CDS	31..6399
Translation	MDAGYSPRKGEYVWVKPQNTTSEFAVPFGARIVRTEKTQTLVCDDRNKQFWVPAGDVLKAMHITSQEDVEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQILPIYTNREIQLYRNKSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYLAAISGKHSWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLSFKPEEVWSILSLLAAILHLGNLRFTATEVANLATAEIDDTPNLQRVAQLLGIPISALNAALTQRTIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQVDQPMNSIGVLDIFGFENFDNNSFEQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQDNQQILDLIGMKPMNLMSLIDEESKFPKGTDQTLLEKLHVQHGNRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRSTNKYLVDIFPHEMPMDTAKKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLLVPPVGPLEQCDCRKLARQICEVALPADSDRQYGKTKLFLRDEDDASLELQRSQLMLKSIVTIQRGIRRVLFRRYMKRYREAIITVQRYWRGRLQRRKYQVMRQGFHRLGACIAAQQLTTKFTMVRCRTIKLQALSRGYLVRKDFQKKLLERRKQNQLKKEELLKLAKMKEAEELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEAAARNALAMAAVQQKRRTKPVKQEAPKAPTLQARNSLPPPPTTLIVAAPLPTRPASAVTRINTIPESPGTIDVESSKQMVDDVFRFLNDEPDAALRKLNNISSGDTIRLPKSVPNNIDTSDFSYLKYAATYFGGGATAQHERKPLKKSLLKHEHPIDEMASKAIWLTILRFMGDLPDVVSSPTLHVFDNENLMSDLASLLNTSDSYKPRLFVRQSQRRIPKPLASGEKEAQEFYQHWLNVPTSHLEKIHFIIGHGIIKNSLRDEILAQICKQLYLNPSRSSYSRGWLLLSLCLSCFPPSKEFEPHLRSFMKQGTAQLQATPSLQRLERTLVNGPRCQPPSLFELHAIRGRHPLRLDIHLMDGQQRRLQVDAASTAREAVNQLCQGMGLTDTFGFGLVMSLNGKLMPLGAGQEHVLDAISECEQRQLDAPWKLYIRKEMFATWYDPSMDPKATQLIYKQILNGLKCGEYRCRSEKDIAMVCALACFVEYGPGEILRLKPSEITAFVPSDLLAPGERAIENWSRLIAATYEKSSYVKEEQNDLLLEAQKRAKEDICLFAHLSWPMRHSRLFEVVRKEGPKLQSDELMLGINSAGLFLIDETEQVLASCCFSEVLKVHVESDDKLHVMTFQHVNFVLQCSSAQDANEVINYMLDNLRQRSSYGVALDPVVEGDLEDCLVLNPGDLIEFEAGVTGAQLMAGNAQDCYRGCVNGQWGQFLAGNVRVLATLTKPSEKLQDILREGRFQEPPKPTPRANYSRRRQHNISQLAESHFREPLDSDKAPLSKFSPEPLKAPLLKAVVKVPPLFQQALVMHHHILKYMGDIARSNLPVNTDLIFQPALQHPLLCDELYCQLMKQLSDNPSSESEKRGWDLLYLATGLVAPSVLVMRELIILLRMRADALADACLKRLKRSLAQGQRKKAPHLIEVEGIQQRCLHIYHKIYFPDDTVEAFEIESHTRGAELIADIAQRLELKSPVGYSIFLKTGDRVYAMPEEEFVFDFITQLIYWLRQQRTIRSISDGQYQLHFMRKLWLNNHPGEDLNGDMIFSYPQELHKYLKGYYPIDCEQASRLAILVYSADHDVSLQRLPEVLTRLIPEDLIPLQTVAEWRQQILPKVHRDHLTEDHAKILFLQELSHFACFGSTFFVVKQQNDDALPETLLIAINSTGFHMLDPTTKEILRSYEYSQLGIWSSGKNHFHIRFGNMIGASKLLCSTTQGYKMDDLLASYVKYFNEHE

Target ORF information:

RefSeq Version	NM_164758.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 28B1, transcript variant B (Myo28B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164758.2

ORF Insert Sequence:

ATGGACGCCG GCTATTCGCC CCGAAAAGGC GAGTATGTGT GGGTGAAGCC GCAAAATACT 
ACCAGCGAAT TCGCAGTGCC CTTTGGGGCT CGAATTGTGC GAACGGAGAA AACACAGACC 
CTAGTTTGCG ATGACCGGAA CAAGCAGTTC TGGGTTCCAG CTGGGGATGT CCTGAAGGCA 
ATGCATATCA CCTCCCAGGA GGATGTTGAG GATATGATCA CTTTGGGTGA CTTACAGGAG 
TACACTATAC TGAGGAACCT GCAGAACAGA TATGCCAAGC AATTAATATA TACCTACACA 
GGCTCCATGT TGGTGGCCAT CAATCCGTAC CAGATTCTGC CTATCTACAC GAATCGCGAG 
ATACAGCTCT ACAGAAATAA GTCCCTTGCT GAACTACCTC CTCACATTTT CGCCATTAGT 
GACAATGCCT TTCAGAGACT ACAAAGACTT AAGGAGAACC AGTGCGTGGT GATCAGCGGG 
GAGTCGGGTG CCGGGAAGAC GGAGAGCACC AAGCTGATCC TTCAGTATCT GGCCGCAATT 
AGTGGCAAAC ACTCGTGGAT CGAGCAGCAA ATCATAGAGG CCAATCCTAT TATGGAGGCC 
TTTGGCAATG CCAAGACTGT ACGGAATGAC AACTCTTCAA GGTTCGGAAA GTACATCGAG 
ATCAGATTCA CGCCACAGGG CGCTATTCAA GGAGCTAGAA TCCAGCAGTA TCTTCTGGAA 
AAATCCCGAA TTGTTTTCCA GAGTCGCGAT GAGCGCAACT ATCACATTTT CTACTGCATG 
TTGGCGGGAC TTTCAACTGC GGAGAGAGAG CGCCTAAAGC TACAGGAGCA ATCGCCAAGT 
CAGTATCATT ACTTGGCCCA AGGAGGATGC TTTACTTTGC CAGGAAGAGG TGATGCCAAG 
GATTTTGCCG ATATTCGAGC GGCTATGAAA GTGCTTTCAT TTAAGCCGGA GGAAGTGTGG 
AGCATACTTA GTTTACTGGC GGCTATTTTG CACTTGGGAA ACCTCAGATT CACAGCCACC 
GAGGTTGCCA ATTTGGCTAC GGCTGAGATA GACGATACAC CAAATCTTCA GAGGGTGGCT 
CAATTACTGG GAATACCCAT ATCTGCTCTG AATGCTGCTC TTACGCAAAG AACCATCTTT 
GTGCATGGTG AGCACGTGAC CACCAGTTTG TCCAAGGAAG CGGCTATTGA GGGTCGAGAT 
GCTTTTGTAA AATCGTTGTA CGATGGAATT TTTGTGCGCA TCGTGCGAAG AATCAATGAG 
ACTATAAACA AGCAAGTGGA TCAGCCCATG AATAGCATAG GTGTTCTAGA TATCTTCGGG 
TTCGAGAACT TCGACAATAA TAGCTTTGAA CAACTGTGCA TCAACTATGC CAACGAAAAT 
CTACAGCAAT TTTTCGTGGG ACATATCTTC AAGATGGAAC AGGATGAGTA CCAGAACGAG 
CATATCAACT GGCAGCACAT TGAGTTCCAG GATAATCAGC AAATCCTCGA TCTAATTGGC 
ATGAAACCCA TGAACTTGAT GTCCCTCATC GACGAAGAGT CCAAGTTTCC CAAGGGCACT 
GATCAAACTC TGCTCGAAAA GCTACATGTT CAGCATGGTA ATCGATCCAT TTACGTGAAA 
GGTAAAACCA CCCAAACGTC TCTATTCGGA ATCCGTCATT ATGCCGGCGT CGTGATGTAC 
AATCCATTGG GCTTTCTTGA AAAGAACAGG GATTCATTCA GCGGAGATCT TAGGACTCTG 
GTGCAGCGAT CAACAAATAA ATATCTGGTG GACATATTCC CCCATGAAAT GCCCATGGAT 
ACGGCCAAGA AGCAGCCCAC TTTGTGCGTG AAATTCAGAA ACTCTCTGGA CATGCTGATG 
CGCACTTTGT CGCAGGCACA TCCCTACTTT ATCCGTTGCA TCAAGCCCAA TGAATACAAG 
GAACCTAAGA ACTTTGACAA GGAGCTGTGC GTGCGGCAGT TGCGCTACTC AGGAATGATG 
GAGACGGCTC GCATTCGAAG GGCTGGCTAT CCGATCCGCC ACGCCTACCG TGCCTTCGTG 
GAGAGATATC GCCTCTTGGT GCCACCGGTG GGACCTTTGG AGCAATGTGA CTGCCGGAAG 
TTGGCGCGGC AGATCTGTGA AGTTGCCCTA CCTGCCGATT CTGATCGTCA GTACGGAAAA 
ACAAAGCTGT TCCTGCGGGA CGAGGACGAC GCTAGCCTGG AACTGCAGCG GTCCCAGCTG 
ATGCTCAAGA GTATCGTGAC AATTCAGCGA GGAATCAGAC GGGTCTTGTT CCGACGTTAC 
ATGAAGAGAT ACCGCGAGGC CATTATTACG GTTCAGAGAT ATTGGCGAGG TCGCCTGCAG 
CGTCGTAAGT ACCAGGTTAT GCGGCAGGGA TTCCATCGCC TGGGAGCCTG TATAGCTGCG 
CAGCAGTTGA CCACCAAGTT CACGATGGTC AGGTGTCGAA CCATAAAACT GCAGGCCTTG 
AGTCGCGGGT ATCTGGTACG CAAGGACTTC CAGAAAAAGT TGTTGGAGCG GCGAAAGCAG 
AACCAGCTGA AGAAGGAGGA GTTACTTAAG TTGGCCAAGA TGAAGGAAGC AGAGGAACTG 
CTAAGACTGC AGCAACTGAA GGAGCAGAAG GAGAGAGAAC AAAGAGAACA GCAGGAAAAA 
CGATTGCAGG AGGAGCAGAG GCTCAAGGCC GAAGCAGCAG CCAGAAATGC ATTGGCCATG 
GCTGCAGTGC AGCAGAAGAG GCGAACTAAG CCCGTCAAGC AAGAGGCTCC AAAGGCTCCA 
ACCCTGCAAG CACGCAACTC CCTGCCACCA CCACCCACCA CACTGATAGT AGCTGCCCCA 
CTACCCACTC GACCGGCCAG TGCCGTTACC AGAATTAATA CCATACCCGA GAGTCCTGGC 
ACCATTGATG TGGAGTCCTC CAAGCAAATG GTGGATGACG TGTTCCGCTT CCTAAACGAT 
GAACCCGATG CAGCCTTGAG GAAACTTAAC AACATCTCTT CTGGAGATAC AATACGTCTA 
CCTAAATCGG TGCCAAATAA CATCGACACC TCCGATTTCA GTTATTTGAA ATATGCGGCT 
ACTTACTTCG GCGGTGGCGC CACTGCACAG CACGAAAGAA AGCCTCTGAA GAAATCTTTG 
TTAAAACATG AACATCCCAT TGATGAGATG GCCTCTAAGG CCATTTGGTT GACTATACTC 
CGCTTTATGG GCGACTTGCC GGATGTTGTT TCCTCGCCTA CTCTTCACGT ATTCGATAAT 
GAAAACTTGA TGAGTGATTT GGCAAGCCTC TTGAACACTT CTGATTCCTA TAAGCCTCGC 
TTATTTGTGC GCCAATCCCA AAGACGTATC CCAAAGCCTT TGGCTAGTGG TGAGAAGGAA 
GCCCAGGAAT TCTATCAACA CTGGCTCAAC GTTCCCACAA GTCACCTGGA GAAAATTCAC 
TTCATCATAG GTCATGGTAT AATAAAGAAT AGCTTACGAG ACGAAATCTT GGCCCAGATC 
TGCAAGCAGC TATATCTTAA CCCAAGTCGG AGCTCCTACT CCCGCGGATG GTTGCTCCTT 
TCGCTATGCC TCAGCTGCTT TCCACCCAGC AAGGAGTTTG AGCCACACTT GCGCAGTTTC 
ATGAAGCAGG GAACTGCCCA ACTGCAGGCC ACTCCATCCC TGCAGCGATT GGAGCGAACC 
CTGGTCAATG GACCACGCTG TCAGCCACCT TCGTTATTTG AGCTCCACGC AATCCGCGGT 
CGCCACCCGC TTAGGTTGGA CATTCACCTG ATGGATGGGC AGCAGAGAAG ATTGCAGGTG 
GATGCGGCCA GTACAGCTCG AGAGGCAGTC AATCAACTTT GTCAGGGTAT GGGGCTAACC 
GACACCTTTG GCTTTGGATT GGTGATGTCA TTGAATGGGA AGCTAATGCC GCTGGGAGCA 
GGTCAGGAGC ATGTCCTGGA TGCCATTTCG GAGTGCGAGC AACGTCAGCT GGATGCACCT 
TGGAAACTAT ACATCCGAAA GGAAATGTTT GCCACTTGGT ATGATCCCTC GATGGATCCC 
AAGGCCACTC AACTTATATA CAAGCAAATT CTAAACGGGT TGAAGTGCGG AGAGTATCGA 
TGTCGCTCCG AGAAGGACAT AGCAATGGTG TGTGCTCTGG CCTGCTTTGT AGAATATGGA 
CCTGGGGAGA TTCTTCGATT GAAGCCATCG GAGATCACTG CATTTGTCCC AAGTGATCTT 
CTAGCTCCCG GCGAGCGAGC CATTGAGAAC TGGAGTCGCC TAATAGCTGC CACCTACGAG 
AAGAGCTCTT ATGTCAAAGA AGAACAGAAT GACCTGCTAT TGGAGGCGCA GAAGAGAGCC 
AAGGAGGATA TATGTCTGTT TGCACATCTC TCCTGGCCTA TGAGGCACTC CCGACTGTTT 
GAAGTGGTTC GCAAAGAGGG ACCAAAACTC CAAAGCGATG AACTTATGCT GGGCATTAAT 
TCCGCGGGCC TTTTCCTTAT CGACGAAACC GAACAGGTGC TGGCATCCTG TTGTTTTAGC 
GAGGTGCTGA AAGTGCACGT GGAATCAGAT GATAAACTGC ATGTTATGAC CTTCCAACAC 
GTCAATTTTG TGCTGCAGTG CAGCTCTGCT CAGGATGCCA ACGAGGTGAT AAACTATATG 
CTGGATAATC TCCGACAGCG TTCAAGCTAT GGCGTAGCTC TTGATCCGGT CGTAGAGGGG 
GATCTAGAGG ATTGTCTAGT GCTAAACCCC GGTGACCTAA TCGAATTCGA GGCAGGTGTA 
ACAGGTGCCC AGTTAATGGC TGGTAATGCA CAAGACTGCT ACAGGGGATG TGTCAATGGC 
CAGTGGGGTC AGTTTTTAGC TGGAAATGTT CGCGTTTTGG CCACGCTCAC CAAGCCGAGT 
GAAAAACTTC AAGATATCCT CCGAGAAGGC CGATTTCAAG AACCCCCGAA ACCAACTCCA 
AGAGCCAATT ACTCTCGTCG TCGTCAACAC AATATCTCTC AGCTGGCTGA GAGCCACTTT 
AGGGAGCCAT TGGACTCCGA TAAGGCACCA CTATCAAAGT TTTCACCTGA GCCTCTGAAA 
GCCCCGCTGC TCAAAGCTGT TGTTAAGGTA CCGCCACTTT TCCAGCAGGC ATTGGTTATG 
CATCATCATA TTCTTAAGTA CATGGGTGAC ATAGCAAGGA GCAATCTACC GGTCAACACG 
GACCTCATTT TCCAGCCAGC ACTGCAGCAT CCGCTGTTGT GTGACGAACT CTACTGCCAG 
CTGATGAAGC AACTGAGCGA TAATCCCTCC AGTGAAAGCG AGAAGCGGGG CTGGGACCTG 
CTTTACTTGG CCACGGGCTT GGTGGCTCCC AGTGTCTTGG TCATGAGGGA GCTGATCATA 
CTACTGCGCA TGCGTGCAGA CGCCCTGGCC GATGCTTGCC TGAAGCGACT GAAGCGATCC 
TTGGCACAGG GCCAGCGAAA AAAGGCACCA CATCTCATAG AGGTCGAGGG AATTCAACAG 
CGATGCCTGC ATATCTACCA CAAGATATAT TTCCCCGACG ATACGGTTGA GGCTTTCGAG 
ATTGAATCAC ACACCCGGGG AGCAGAGCTC ATAGCGGACA TAGCCCAGAG ATTGGAGCTC 
AAATCACCTG TGGGTTATAG TATTTTCTTG AAGACAGGCG ACAGGGTCTA TGCTATGCCG 
GAAGAGGAGT TCGTTTTTGA TTTCATCACC CAACTCATCT ACTGGCTAAG ACAGCAGAGG 
ACCATACGCT CCATATCCGA TGGCCAATAC CAACTGCACT TCATGAGAAA GTTATGGTTA 
AACAACCATC CAGGCGAAGA CCTCAATGGA GATATGATCT TTAGCTATCC CCAAGAGCTG 
CACAAATACT TGAAGGGCTA CTATCCCATC GATTGCGAGC AGGCCTCGCG TTTGGCAATT 
CTTGTGTACA GTGCAGATCA TGACGTTAGC TTGCAGCGGC TGCCCGAAGT GCTGACACGT 
TTAATTCCTG AAGACCTAAT ACCGCTTCAA ACGGTGGCCG AATGGAGGCA GCAGATCCTG 
CCCAAGGTCC ACCGCGATCA TTTAACGGAA GATCACGCCA AGATTTTGTT CCTTCAAGAG 
CTGTCCCATT TCGCTTGCTT CGGTTCCACA TTCTTTGTGG TGAAGCAACA AAATGACGAT 
GCCCTTCCCG AAACTCTTTT AATCGCAATT AACAGCACAG GTTTCCATAT GCTGGATCCG 
ACAACTAAGG AGATCCTTCG CAGCTATGAA TACTCCCAGT TGGGTATTTG GAGCTCGGGT 
AAGAACCACT TCCACATTCG TTTTGGTAAC ATGATCGGAG CATCGAAGCT GCTGTGCAGC 
ACCACCCAAG GATACAAAAT GGACGATTTG CTGGCCTCTT ATGTTAAGTA TTTTAATGAA 
CACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo28B1 ( NM_144373.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652630.2 Drosophila melanogaster myosin 28B1, transcript variant A (Myo28B1), mRNA.

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CloneID	OFa15333
Clone ID Related Accession (Same CDS sequence)	NM_144373.4
Accession Version	NM_144373.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 28B1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_144373.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAAT TTGTGCGGCA ACATGGCGAG TATGTGTGGG TGAAGCCGCA AAATACTACC 
AGCGAATTCG CAGTGCCCTT TGGGGCTCGA ATTGTGCGAA CGGAGAAAAC ACAGACCCTA 
GTTTGCGATG ACCGGAACAA GCAGTTCTGG GTTCCAGCTG GGGATGTCCT GAAGGCAATG 
CATATCACCT CCCAGGAGGA TGTTGAGGAT ATGATCACTT TGGGTGACTT ACAGGAGTAC 
ACTATACTGA GGAACCTGCA GAACAGATAT GCCAAGCAAT TAATATATAC CTACACAGGC 
TCCATGTTGG TGGCCATCAA TCCGTACCAG ATTCTGCCTA TCTACACGAA TCGCGAGATA 
CAGCTCTACA GAAATAAGTC CCTTGCTGAA CTACCTCCTC ACATTTTCGC CATTAGTGAC 
AATGCCTTTC AGAGACTACA AAGACTTAAG GAGAACCAGT GCGTGGTGAT CAGCGGGGAG 
TCGGGTGCCG GGAAGACGGA GAGCACCAAG CTGATCCTTC AGTATCTGGC CGCAATTAGT 
GGCAAACACT CGTGGATCGA GCAGCAAATC ATAGAGGCCA ATCCTATTAT GGAGGCCTTT 
GGCAATGCCA AGACTGTACG GAATGACAAC TCTTCAAGGT TCGGAAAGTA CATCGAGATC 
AGATTCACGC CACAGGGCGC TATTCAAGGA GCTAGAATCC AGCAGTATCT TCTGGAAAAA 
TCCCGAATTG TTTTCCAGAG TCGCGATGAG CGCAACTATC ACATTTTCTA CTGCATGTTG 
GCGGGACTTT CAACTGCGGA GAGAGAGCGC CTAAAGCTAC AGGAGCAATC GCCAAGTCAG 
TATCATTACT TGGCCCAAGG AGGATGCTTT ACTTTGCCAG GAAGAGGTGA TGCCAAGGAT 
TTTGCCGATA TTCGAGCGGC TATGAAAGTG CTTTCATTTA AGCCGGAGGA AGTGTGGAGC 
ATACTTAGTT TACTGGCGGC TATTTTGCAC TTGGGAAACC TCAGATTCAC AGCCACCGAG 
GTTGCCAATT TGGCTACGGC TGAGATAGAC GATACACCAA ATCTTCAGAG GGTGGCTCAA 
TTACTGGGAA TACCCATATC TGCTCTGAAT GCTGCTCTTA CGCAAAGAAC CATCTTTGTG 
CATGGTGAGC ACGTGACCAC CAGTTTGTCC AAGGAAGCGG CTATTGAGGG TCGAGATGCT 
TTTGTAAAAT CGTTGTACGA TGGAATTTTT GTGCGCATCG TGCGAAGAAT CAATGAGACT 
ATAAACAAGC AAGTGGATCA GCCCATGAAT AGCATAGGTG TTCTAGATAT CTTCGGGTTC 
GAGAACTTCG ACAATAATAG CTTTGAACAA CTGTGCATCA ACTATGCCAA CGAAAATCTA 
CAGCAATTTT TCGTGGGACA TATCTTCAAG ATGGAACAGG ATGAGTACCA GAACGAGCAT 
ATCAACTGGC AGCACATTGA GTTCCAGGAT AATCAGCAAA TCCTCGATCT AATTGGCATG 
AAACCCATGA ACTTGATGTC CCTCATCGAC GAAGAGTCCA AGTTTCCCAA GGGCACTGAT 
CAAACTCTGC TCGAAAAGCT ACATGTTCAG CATGGTAATC GATCCATTTA CGTGAAAGGT 
AAAACCACCC AAACGTCTCT ATTCGGAATC CGTCATTATG CCGGCGTCGT GATGTACAAT 
CCATTGGGCT TTCTTGAAAA GAACAGGGAT TCATTCAGCG GAGATCTTAG GACTCTGGTG 
CAGCGATCAA CAAATAAATA TCTGGTGGAC ATATTCCCCC ATGAAATGCC CATGGATACG 
GCCAAGAAGC AGCCCACTTT GTGCGTGAAA TTCAGAAACT CTCTGGACAT GCTGATGCGC 
ACTTTGTCGC AGGCACATCC CTACTTTATC CGTTGCATCA AGCCCAATGA ATACAAGGAA 
CCTAAGAACT TTGACAAGGA GCTGTGCGTG CGGCAGTTGC GCTACTCAGG AATGATGGAG 
ACGGCTCGCA TTCGAAGGGC TGGCTATCCG ATCCGCCACG CCTACCGTGC CTTCGTGGAG 
AGATATCGCC TCTTGGTGCC ACCGGTGGGA CCTTTGGAGC AATGTGACTG CCGGAAGTTG 
GCGCGGCAGA TCTGTGAAGT TGCCCTACCT GCCGATTCTG ATCGTCAGTA CGGAAAAACA 
AAGCTGTTCC TGCGGGACGA GGACGACGCT AGCCTGGAAC TGCAGCGGTC CCAGCTGATG 
CTCAAGAGTA TCGTGACAAT TCAGCGAGGA ATCAGACGGG TCTTGTTCCG ACGTTACATG 
AAGAGATACC GCGAGGCCAT TATTACGGTT CAGAGATATT GGCGAGGTCG CCTGCAGCGT 
CGTAAGTACC AGGTTATGCG GCAGGGATTC CATCGCCTGG GAGCCTGTAT AGCTGCGCAG 
CAGTTGACCA CCAAGTTCAC GATGGTCAGG TGTCGAACCA TAAAACTGCA GGCCTTGAGT 
CGCGGGTATC TGGTACGCAA GGACTTCCAG AAAAAGTTGT TGGAGCGGCG AAAGCAGAAC 
CAGCTGAAGA AGGAGGAGTT ACTTAAGTTG GCCAAGATGA AGGAAGCAGA GGAACTGCTA 
AGACTGCAGC AACTGAAGGA GCAGAAGGAG AGAGAACAAA GAGAACAGCA GGAAAAACGA 
TTGCAGGAGG AGCAGAGGCT CAAGGCCGAA GCAGCAGCCA GAAATGCATT GGCCATGGCT 
GCAGTGCAGC AGAAGAGGCG AACTAAGCCC GTCAAGCAAG AGGCTCCAAA GGCTCCAACC 
CTGCAAGCAC GCAACTCCCT GCCACCACCA CCCACCACAC TGATAGTAGC TGCCCCACTA 
CCCACTCGAC CGGCCAGTGC CGTTACCAGA ATTAATACCA TACCCGAGAG TCCTGGCACC 
ATTGATGTGG AGTCCTCCAA GCAAATGGTG GATGACGTGT TCCGCTTCCT AAACGATGAA 
CCCGATGCAG CCTTGAGGAA ACTTAACAAC ATCTCTTCTG GAGATACAAT ACGTCTACCT 
AAATCGGTGC CAAATAACAT CGACACCTCC GATTTCAGTT ATTTGAAATA TGCGGCTACT 
TACTTCGGCG GTGGCGCCAC TGCACAGCAC GAAAGAAAGC CTCTGAAGAA ATCTTTGTTA 
AAACATGAAC ATCCCATTGA TGAGATGGCC TCTAAGGCCA TTTGGTTGAC TATACTCCGC 
TTTATGGGCG ACTTGCCGGA TGTTGTTTCC TCGCCTACTC TTCACGTATT CGATAATGAA 
AACTTGATGA GTGATTTGGC AAGCCTCTTG AACACTTCTG ATTCCTATAA GCCTCGCTTA 
TTTGTGCGCC AATCCCAAAG ACGTATCCCA AAGCCTTTGG CTAGTGGTGA GAAGGAAGCC 
CAGGAATTCT ATCAACACTG GCTCAACGTT CCCACAAGTC ACCTGGAGAA AATTCACTTC 
ATCATAGGTC ATGGTATAAT AAAGAATAGC TTACGAGACG AAATCTTGGC CCAGATCTGC 
AAGCAGCTAT ATCTTAACCC AAGTCGGAGC TCCTACTCCC GCGGATGGTT GCTCCTTTCG 
CTATGCCTCA GCTGCTTTCC ACCCAGCAAG GAGTTTGAGC CACACTTGCG CAGTTTCATG 
AAGCAGGGAA CTGCCCAACT GCAGGCCACT CCATCCCTGC AGCGATTGGA GCGAACCCTG 
GTCAATGGAC CACGCTGTCA GCCACCTTCG TTATTTGAGC TCCACGCAAT CCGCGGTCGC 
CACCCGCTTA GGTTGGACAT TCACCTGATG GATGGGCAGC AGAGAAGATT GCAGGTGGAT 
GCGGCCAGTA CAGCTCGAGA GGCAGTCAAT CAACTTTGTC AGGGTATGGG GCTAACCGAC 
ACCTTTGGCT TTGGATTGGT GATGTCATTG AATGGGAAGC TAATGCCGCT GGGAGCAGGT 
CAGGAGCATG TCCTGGATGC CATTTCGGAG TGCGAGCAAC GTCAGCTGGA TGCACCTTGG 
AAACTATACA TCCGAAAGGA AATGTTTGCC ACTTGGTATG ATCCCTCGAT GGATCCCAAG 
GCCACTCAAC TTATATACAA GCAAATTCTA AACGGGTTGA AGTGCGGAGA GTATCGATGT 
CGCTCCGAGA AGGACATAGC AATGGTGTGT GCTCTGGCCT GCTTTGTAGA ATATGGACCT 
GGGGAGATTC TTCGATTGAA GCCATCGGAG ATCACTGCAT TTGTCCCAAG TGATCTTCTA 
GCTCCCGGCG AGCGAGCCAT TGAGAACTGG AGTCGCCTAA TAGCTGCCAC CTACGAGAAG 
AGCTCTTATG TCAAAGAAGA ACAGAATGAC CTGCTATTGG AGGCGCAGAA GAGAGCCAAG 
GAGGATATAT GTCTGTTTGC ACATCTCTCC TGGCCTATGA GGCACTCCCG ACTGTTTGAA 
GTGGTTCGCA AAGAGGGACC AAAACTCCAA AGCGATGAAC TTATGCTGGG CATTAATTCC 
GCGGGCCTTT TCCTTATCGA CGAAACCGAA CAGGTGCTGG CATCCTGTTG TTTTAGCGAG 
GTGCTGAAAG TGCACGTGGA ATCAGATGAT AAACTGCATG TTATGACCTT CCAACACGTC 
AATTTTGTGC TGCAGTGCAG CTCTGCTCAG GATGCCAACG AGGTGATAAA CTATATGCTG 
GATAATCTCC GACAGCGTTC AAGCTATGGC GTAGCTCTTG ATCCGGTCGT AGAGGGGGAT 
CTAGAGGATT GTCTAGTGCT AAACCCCGGT GACCTAATCG AATTCGAGGC AGGTGTAACA 
GGTGCCCAGT TAATGGCTGG TAATGCACAA GACTGCTACA GGGGATGTGT CAATGGCCAG 
TGGGGTCAGT TTTTAGCTGG AAATGTTCGC GTTTTGGCCA CGCTCACCAA GCCGAGTGAA 
AAACTTCAAG ATATCCTCCG AGAAGGCCGA TTTCAAGAAC CCCCGAAACC AACTCCAAGA 
GCCAATTACT CTCGTCGTCG TCAACACAAT ATCTCTCAGC TGGCTGAGAG CCACTTTAGG 
GAGCCATTGG ACTCCGATAA GGCACCACTA TCAAAGTTTT CACCTGAGCC TCTGAAAGCC 
CCGCTGCTCA AAGCTGTTGT TAAGGTACCG CCACTTTTCC AGCAGGCATT GGTTATGCAT 
CATCATATTC TTAAGTACAT GGGTGACATA GCAAGGAGCA ATCTACCGGT CAACACGGAC 
CTCATTTTCC AGCCAGCACT GCAGCATCCG CTGTTGTGTG ACGAACTCTA CTGCCAGCTG 
ATGAAGCAAC TGAGCGATAA TCCCTCCAGT GAAAGCGAGA AGCGGGGCTG GGACCTGCTT 
TACTTGGCCA CGGGCTTGGT GGCTCCCAGT GTCTTGGTCA TGAGGGAGCT GATCATACTA 
CTGCGCATGC GTGCAGACGC CCTGGCCGAT GCTTGCCTGA AGCGACTGAA GCGATCCTTG 
GCACAGGGCC AGCGAAAAAA GGCACCACAT CTCATAGAGG TCGAGGGAAT TCAACAGCGA 
TGCCTGCATA TCTACCACAA GATATATTTC CCCGACGATA CGGTTGAGGC TTTCGAGATT 
GAATCACACA CCCGGGGAGC AGAGCTCATA GCGGACATAG CCCAGAGATT GGAGCTCAAA 
TCACCTGTGG GTTATAGTAT TTTCTTGAAG ACAGGCGACA GGGTCTATGC TATGCCGGAA 
GAGGAGTTCG TTTTTGATTT CATCACCCAA CTCATCTACT GGCTAAGACA GCAGAGGACC 
ATACGCTCCA TATCCGATGG CCAATACCAA CTGCACTTCA TGAGAAAGTT ATGGTTAAAC 
AACCATCCAG GCGAAGACCT CAATGGAGAT ATGATCTTTA GCTATCCCCA AGAGCTGCAC 
AAATACTTGA AGGGCTACTA TCCCATCGAT TGCGAGCAGG CCTCGCGTTT GGCAATTCTT 
GTGTACAGTG CAGATCATGA CGTTAGCTTG CAGCGGCTGC CCGAAGTGCT GACACGTTTA 
ATTCCTGAAG ACCTAATACC GCTTCAAACG GTGGCCGAAT GGAGGCAGCA GATCCTGCCC 
AAGGTCCACC GCGATCATTT AACGGAAGAT CACGCCAAGA TTTTGTTCCT TCAAGAGCTG 
TCCCATTTCG CTTGCTTCGG TTCCACATTC TTTGTGGTGA AGCAACAAAA TGACGATGCC 
CTTCCCGAAA CTCTTTTAAT CGCAATTAAC AGCACAGGTT TCCATATGCT GGATCCGACA 
ACTAAGGAGA TCCTTCGCAG CTATGAATAC TCCCAGTTGG GTATTTGGAG CTCGGGTAAG 
AACCACTTCC ACATTCGTTT TGGTAACATG ATCGGAGCAT CGAAGCTGCT GTGCAGCACC 
ACCCAAGGAT ACAAAATGGA CGATTTGCTG GCCTCTTATG TTAAGTATTT TAATGAACAC 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652630.2
CDS	179..6544
Translation	MSEFVRQHGEYVWVKPQNTTSEFAVPFGARIVRTEKTQTLVCDDRNKQFWVPAGDVLKAMHITSQEDVEDMITLGDLQEYTILRNLQNRYAKQLIYTYTGSMLVAINPYQILPIYTNREIQLYRNKSLAELPPHIFAISDNAFQRLQRLKENQCVVISGESGAGKTESTKLILQYLAAISGKHSWIEQQIIEANPIMEAFGNAKTVRNDNSSRFGKYIEIRFTPQGAIQGARIQQYLLEKSRIVFQSRDERNYHIFYCMLAGLSTAERERLKLQEQSPSQYHYLAQGGCFTLPGRGDAKDFADIRAAMKVLSFKPEEVWSILSLLAAILHLGNLRFTATEVANLATAEIDDTPNLQRVAQLLGIPISALNAALTQRTIFVHGEHVTTSLSKEAAIEGRDAFVKSLYDGIFVRIVRRINETINKQVDQPMNSIGVLDIFGFENFDNNSFEQLCINYANENLQQFFVGHIFKMEQDEYQNEHINWQHIEFQDNQQILDLIGMKPMNLMSLIDEESKFPKGTDQTLLEKLHVQHGNRSIYVKGKTTQTSLFGIRHYAGVVMYNPLGFLEKNRDSFSGDLRTLVQRSTNKYLVDIFPHEMPMDTAKKQPTLCVKFRNSLDMLMRTLSQAHPYFIRCIKPNEYKEPKNFDKELCVRQLRYSGMMETARIRRAGYPIRHAYRAFVERYRLLVPPVGPLEQCDCRKLARQICEVALPADSDRQYGKTKLFLRDEDDASLELQRSQLMLKSIVTIQRGIRRVLFRRYMKRYREAIITVQRYWRGRLQRRKYQVMRQGFHRLGACIAAQQLTTKFTMVRCRTIKLQALSRGYLVRKDFQKKLLERRKQNQLKKEELLKLAKMKEAEELLRLQQLKEQKEREQREQQEKRLQEEQRLKAEAAARNALAMAAVQQKRRTKPVKQEAPKAPTLQARNSLPPPPTTLIVAAPLPTRPASAVTRINTIPESPGTIDVESSKQMVDDVFRFLNDEPDAALRKLNNISSGDTIRLPKSVPNNIDTSDFSYLKYAATYFGGGATAQHERKPLKKSLLKHEHPIDEMASKAIWLTILRFMGDLPDVVSSPTLHVFDNENLMSDLASLLNTSDSYKPRLFVRQSQRRIPKPLASGEKEAQEFYQHWLNVPTSHLEKIHFIIGHGIIKNSLRDEILAQICKQLYLNPSRSSYSRGWLLLSLCLSCFPPSKEFEPHLRSFMKQGTAQLQATPSLQRLERTLVNGPRCQPPSLFELHAIRGRHPLRLDIHLMDGQQRRLQVDAASTAREAVNQLCQGMGLTDTFGFGLVMSLNGKLMPLGAGQEHVLDAISECEQRQLDAPWKLYIRKEMFATWYDPSMDPKATQLIYKQILNGLKCGEYRCRSEKDIAMVCALACFVEYGPGEILRLKPSEITAFVPSDLLAPGERAIENWSRLIAATYEKSSYVKEEQNDLLLEAQKRAKEDICLFAHLSWPMRHSRLFEVVRKEGPKLQSDELMLGINSAGLFLIDETEQVLASCCFSEVLKVHVESDDKLHVMTFQHVNFVLQCSSAQDANEVINYMLDNLRQRSSYGVALDPVVEGDLEDCLVLNPGDLIEFEAGVTGAQLMAGNAQDCYRGCVNGQWGQFLAGNVRVLATLTKPSEKLQDILREGRFQEPPKPTPRANYSRRRQHNISQLAESHFREPLDSDKAPLSKFSPEPLKAPLLKAVVKVPPLFQQALVMHHHILKYMGDIARSNLPVNTDLIFQPALQHPLLCDELYCQLMKQLSDNPSSESEKRGWDLLYLATGLVAPSVLVMRELIILLRMRADALADACLKRLKRSLAQGQRKKAPHLIEVEGIQQRCLHIYHKIYFPDDTVEAFEIESHTRGAELIADIAQRLELKSPVGYSIFLKTGDRVYAMPEEEFVFDFITQLIYWLRQQRTIRSISDGQYQLHFMRKLWLNNHPGEDLNGDMIFSYPQELHKYLKGYYPIDCEQASRLAILVYSADHDVSLQRLPEVLTRLIPEDLIPLQTVAEWRQQILPKVHRDHLTEDHAKILFLQELSHFACFGSTFFVVKQQNDDALPETLLIAINSTGFHMLDPTTKEILRSYEYSQLGIWSSGKNHFHIRFGNMIGASKLLCSTTQGYKMDDLLASYVKYFNEHE

Target ORF information:

RefSeq Version	NM_144373.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 28B1, transcript variant A (Myo28B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144373.4

ORF Insert Sequence:

ATGTCCGAAT TTGTGCGGCA ACATGGCGAG TATGTGTGGG TGAAGCCGCA AAATACTACC 
AGCGAATTCG CAGTGCCCTT TGGGGCTCGA ATTGTGCGAA CGGAGAAAAC ACAGACCCTA 
GTTTGCGATG ACCGGAACAA GCAGTTCTGG GTTCCAGCTG GGGATGTCCT GAAGGCAATG 
CATATCACCT CCCAGGAGGA TGTTGAGGAT ATGATCACTT TGGGTGACTT ACAGGAGTAC 
ACTATACTGA GGAACCTGCA GAACAGATAT GCCAAGCAAT TAATATATAC CTACACAGGC 
TCCATGTTGG TGGCCATCAA TCCGTACCAG ATTCTGCCTA TCTACACGAA TCGCGAGATA 
CAGCTCTACA GAAATAAGTC CCTTGCTGAA CTACCTCCTC ACATTTTCGC CATTAGTGAC 
AATGCCTTTC AGAGACTACA AAGACTTAAG GAGAACCAGT GCGTGGTGAT CAGCGGGGAG 
TCGGGTGCCG GGAAGACGGA GAGCACCAAG CTGATCCTTC AGTATCTGGC CGCAATTAGT 
GGCAAACACT CGTGGATCGA GCAGCAAATC ATAGAGGCCA ATCCTATTAT GGAGGCCTTT 
GGCAATGCCA AGACTGTACG GAATGACAAC TCTTCAAGGT TCGGAAAGTA CATCGAGATC 
AGATTCACGC CACAGGGCGC TATTCAAGGA GCTAGAATCC AGCAGTATCT TCTGGAAAAA 
TCCCGAATTG TTTTCCAGAG TCGCGATGAG CGCAACTATC ACATTTTCTA CTGCATGTTG 
GCGGGACTTT CAACTGCGGA GAGAGAGCGC CTAAAGCTAC AGGAGCAATC GCCAAGTCAG 
TATCATTACT TGGCCCAAGG AGGATGCTTT ACTTTGCCAG GAAGAGGTGA TGCCAAGGAT 
TTTGCCGATA TTCGAGCGGC TATGAAAGTG CTTTCATTTA AGCCGGAGGA AGTGTGGAGC 
ATACTTAGTT TACTGGCGGC TATTTTGCAC TTGGGAAACC TCAGATTCAC AGCCACCGAG 
GTTGCCAATT TGGCTACGGC TGAGATAGAC GATACACCAA ATCTTCAGAG GGTGGCTCAA 
TTACTGGGAA TACCCATATC TGCTCTGAAT GCTGCTCTTA CGCAAAGAAC CATCTTTGTG 
CATGGTGAGC ACGTGACCAC CAGTTTGTCC AAGGAAGCGG CTATTGAGGG TCGAGATGCT 
TTTGTAAAAT CGTTGTACGA TGGAATTTTT GTGCGCATCG TGCGAAGAAT CAATGAGACT 
ATAAACAAGC AAGTGGATCA GCCCATGAAT AGCATAGGTG TTCTAGATAT CTTCGGGTTC 
GAGAACTTCG ACAATAATAG CTTTGAACAA CTGTGCATCA ACTATGCCAA CGAAAATCTA 
CAGCAATTTT TCGTGGGACA TATCTTCAAG ATGGAACAGG ATGAGTACCA GAACGAGCAT 
ATCAACTGGC AGCACATTGA GTTCCAGGAT AATCAGCAAA TCCTCGATCT AATTGGCATG 
AAACCCATGA ACTTGATGTC CCTCATCGAC GAAGAGTCCA AGTTTCCCAA GGGCACTGAT 
CAAACTCTGC TCGAAAAGCT ACATGTTCAG CATGGTAATC GATCCATTTA CGTGAAAGGT 
AAAACCACCC AAACGTCTCT ATTCGGAATC CGTCATTATG CCGGCGTCGT GATGTACAAT 
CCATTGGGCT TTCTTGAAAA GAACAGGGAT TCATTCAGCG GAGATCTTAG GACTCTGGTG 
CAGCGATCAA CAAATAAATA TCTGGTGGAC ATATTCCCCC ATGAAATGCC CATGGATACG 
GCCAAGAAGC AGCCCACTTT GTGCGTGAAA TTCAGAAACT CTCTGGACAT GCTGATGCGC 
ACTTTGTCGC AGGCACATCC CTACTTTATC CGTTGCATCA AGCCCAATGA ATACAAGGAA 
CCTAAGAACT TTGACAAGGA GCTGTGCGTG CGGCAGTTGC GCTACTCAGG AATGATGGAG 
ACGGCTCGCA TTCGAAGGGC TGGCTATCCG ATCCGCCACG CCTACCGTGC CTTCGTGGAG 
AGATATCGCC TCTTGGTGCC ACCGGTGGGA CCTTTGGAGC AATGTGACTG CCGGAAGTTG 
GCGCGGCAGA TCTGTGAAGT TGCCCTACCT GCCGATTCTG ATCGTCAGTA CGGAAAAACA 
AAGCTGTTCC TGCGGGACGA GGACGACGCT AGCCTGGAAC TGCAGCGGTC CCAGCTGATG 
CTCAAGAGTA TCGTGACAAT TCAGCGAGGA ATCAGACGGG TCTTGTTCCG ACGTTACATG 
AAGAGATACC GCGAGGCCAT TATTACGGTT CAGAGATATT GGCGAGGTCG CCTGCAGCGT 
CGTAAGTACC AGGTTATGCG GCAGGGATTC CATCGCCTGG GAGCCTGTAT AGCTGCGCAG 
CAGTTGACCA CCAAGTTCAC GATGGTCAGG TGTCGAACCA TAAAACTGCA GGCCTTGAGT 
CGCGGGTATC TGGTACGCAA GGACTTCCAG AAAAAGTTGT TGGAGCGGCG AAAGCAGAAC 
CAGCTGAAGA AGGAGGAGTT ACTTAAGTTG GCCAAGATGA AGGAAGCAGA GGAACTGCTA 
AGACTGCAGC AACTGAAGGA GCAGAAGGAG AGAGAACAAA GAGAACAGCA GGAAAAACGA 
TTGCAGGAGG AGCAGAGGCT CAAGGCCGAA GCAGCAGCCA GAAATGCATT GGCCATGGCT 
GCAGTGCAGC AGAAGAGGCG AACTAAGCCC GTCAAGCAAG AGGCTCCAAA GGCTCCAACC 
CTGCAAGCAC GCAACTCCCT GCCACCACCA CCCACCACAC TGATAGTAGC TGCCCCACTA 
CCCACTCGAC CGGCCAGTGC CGTTACCAGA ATTAATACCA TACCCGAGAG TCCTGGCACC 
ATTGATGTGG AGTCCTCCAA GCAAATGGTG GATGACGTGT TCCGCTTCCT AAACGATGAA 
CCCGATGCAG CCTTGAGGAA ACTTAACAAC ATCTCTTCTG GAGATACAAT ACGTCTACCT 
AAATCGGTGC CAAATAACAT CGACACCTCC GATTTCAGTT ATTTGAAATA TGCGGCTACT 
TACTTCGGCG GTGGCGCCAC TGCACAGCAC GAAAGAAAGC CTCTGAAGAA ATCTTTGTTA 
AAACATGAAC ATCCCATTGA TGAGATGGCC TCTAAGGCCA TTTGGTTGAC TATACTCCGC 
TTTATGGGCG ACTTGCCGGA TGTTGTTTCC TCGCCTACTC TTCACGTATT CGATAATGAA 
AACTTGATGA GTGATTTGGC AAGCCTCTTG AACACTTCTG ATTCCTATAA GCCTCGCTTA 
TTTGTGCGCC AATCCCAAAG ACGTATCCCA AAGCCTTTGG CTAGTGGTGA GAAGGAAGCC 
CAGGAATTCT ATCAACACTG GCTCAACGTT CCCACAAGTC ACCTGGAGAA AATTCACTTC 
ATCATAGGTC ATGGTATAAT AAAGAATAGC TTACGAGACG AAATCTTGGC CCAGATCTGC 
AAGCAGCTAT ATCTTAACCC AAGTCGGAGC TCCTACTCCC GCGGATGGTT GCTCCTTTCG 
CTATGCCTCA GCTGCTTTCC ACCCAGCAAG GAGTTTGAGC CACACTTGCG CAGTTTCATG 
AAGCAGGGAA CTGCCCAACT GCAGGCCACT CCATCCCTGC AGCGATTGGA GCGAACCCTG 
GTCAATGGAC CACGCTGTCA GCCACCTTCG TTATTTGAGC TCCACGCAAT CCGCGGTCGC 
CACCCGCTTA GGTTGGACAT TCACCTGATG GATGGGCAGC AGAGAAGATT GCAGGTGGAT 
GCGGCCAGTA CAGCTCGAGA GGCAGTCAAT CAACTTTGTC AGGGTATGGG GCTAACCGAC 
ACCTTTGGCT TTGGATTGGT GATGTCATTG AATGGGAAGC TAATGCCGCT GGGAGCAGGT 
CAGGAGCATG TCCTGGATGC CATTTCGGAG TGCGAGCAAC GTCAGCTGGA TGCACCTTGG 
AAACTATACA TCCGAAAGGA AATGTTTGCC ACTTGGTATG ATCCCTCGAT GGATCCCAAG 
GCCACTCAAC TTATATACAA GCAAATTCTA AACGGGTTGA AGTGCGGAGA GTATCGATGT 
CGCTCCGAGA AGGACATAGC AATGGTGTGT GCTCTGGCCT GCTTTGTAGA ATATGGACCT 
GGGGAGATTC TTCGATTGAA GCCATCGGAG ATCACTGCAT TTGTCCCAAG TGATCTTCTA 
GCTCCCGGCG AGCGAGCCAT TGAGAACTGG AGTCGCCTAA TAGCTGCCAC CTACGAGAAG 
AGCTCTTATG TCAAAGAAGA ACAGAATGAC CTGCTATTGG AGGCGCAGAA GAGAGCCAAG 
GAGGATATAT GTCTGTTTGC ACATCTCTCC TGGCCTATGA GGCACTCCCG ACTGTTTGAA 
GTGGTTCGCA AAGAGGGACC AAAACTCCAA AGCGATGAAC TTATGCTGGG CATTAATTCC 
GCGGGCCTTT TCCTTATCGA CGAAACCGAA CAGGTGCTGG CATCCTGTTG TTTTAGCGAG 
GTGCTGAAAG TGCACGTGGA ATCAGATGAT AAACTGCATG TTATGACCTT CCAACACGTC 
AATTTTGTGC TGCAGTGCAG CTCTGCTCAG GATGCCAACG AGGTGATAAA CTATATGCTG 
GATAATCTCC GACAGCGTTC AAGCTATGGC GTAGCTCTTG ATCCGGTCGT AGAGGGGGAT 
CTAGAGGATT GTCTAGTGCT AAACCCCGGT GACCTAATCG AATTCGAGGC AGGTGTAACA 
GGTGCCCAGT TAATGGCTGG TAATGCACAA GACTGCTACA GGGGATGTGT CAATGGCCAG 
TGGGGTCAGT TTTTAGCTGG AAATGTTCGC GTTTTGGCCA CGCTCACCAA GCCGAGTGAA 
AAACTTCAAG ATATCCTCCG AGAAGGCCGA TTTCAAGAAC CCCCGAAACC AACTCCAAGA 
GCCAATTACT CTCGTCGTCG TCAACACAAT ATCTCTCAGC TGGCTGAGAG CCACTTTAGG 
GAGCCATTGG ACTCCGATAA GGCACCACTA TCAAAGTTTT CACCTGAGCC TCTGAAAGCC 
CCGCTGCTCA AAGCTGTTGT TAAGGTACCG CCACTTTTCC AGCAGGCATT GGTTATGCAT 
CATCATATTC TTAAGTACAT GGGTGACATA GCAAGGAGCA ATCTACCGGT CAACACGGAC 
CTCATTTTCC AGCCAGCACT GCAGCATCCG CTGTTGTGTG ACGAACTCTA CTGCCAGCTG 
ATGAAGCAAC TGAGCGATAA TCCCTCCAGT GAAAGCGAGA AGCGGGGCTG GGACCTGCTT 
TACTTGGCCA CGGGCTTGGT GGCTCCCAGT GTCTTGGTCA TGAGGGAGCT GATCATACTA 
CTGCGCATGC GTGCAGACGC CCTGGCCGAT GCTTGCCTGA AGCGACTGAA GCGATCCTTG 
GCACAGGGCC AGCGAAAAAA GGCACCACAT CTCATAGAGG TCGAGGGAAT TCAACAGCGA 
TGCCTGCATA TCTACCACAA GATATATTTC CCCGACGATA CGGTTGAGGC TTTCGAGATT 
GAATCACACA CCCGGGGAGC AGAGCTCATA GCGGACATAG CCCAGAGATT GGAGCTCAAA 
TCACCTGTGG GTTATAGTAT TTTCTTGAAG ACAGGCGACA GGGTCTATGC TATGCCGGAA 
GAGGAGTTCG TTTTTGATTT CATCACCCAA CTCATCTACT GGCTAAGACA GCAGAGGACC 
ATACGCTCCA TATCCGATGG CCAATACCAA CTGCACTTCA TGAGAAAGTT ATGGTTAAAC 
AACCATCCAG GCGAAGACCT CAATGGAGAT ATGATCTTTA GCTATCCCCA AGAGCTGCAC 
AAATACTTGA AGGGCTACTA TCCCATCGAT TGCGAGCAGG CCTCGCGTTT GGCAATTCTT 
GTGTACAGTG CAGATCATGA CGTTAGCTTG CAGCGGCTGC CCGAAGTGCT GACACGTTTA 
ATTCCTGAAG ACCTAATACC GCTTCAAACG GTGGCCGAAT GGAGGCAGCA GATCCTGCCC 
AAGGTCCACC GCGATCATTT AACGGAAGAT CACGCCAAGA TTTTGTTCCT TCAAGAGCTG 
TCCCATTTCG CTTGCTTCGG TTCCACATTC TTTGTGGTGA AGCAACAAAA TGACGATGCC 
CTTCCCGAAA CTCTTTTAAT CGCAATTAAC AGCACAGGTT TCCATATGCT GGATCCGACA 
ACTAAGGAGA TCCTTCGCAG CTATGAATAC TCCCAGTTGG GTATTTGGAG CTCGGGTAAG 
AACCACTTCC ACATTCGTTT TGGTAACATG ATCGGAGCAT CGAAGCTGCT GTGCAGCACC 
ACCCAAGGAT ACAAAATGGA CGATTTGCTG GCCTCTTATG TTAAGTATTT TAATGAACAC 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Myo28B1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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