Sulf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sulf1
Entrez Gene ID	53437
Full Name	Sulfated
Synonyms	CG6725,DSulf,DSulf1,Dmel\CG6725,Dsulf1,Neu5,sulf1
General protein information	Preferred Names Sulfated Names CG6725 gene product from transcript CG6725-RC CG6725-PA CG6725-PB CG6725-PC Sulf1-PA Sulf1-PB Sulf1-PC sulfated
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B12-89B13

mRNA and Protein(s)

mRNA	Protein	Name
NM_080248.3	NP_524987.1	sulfated, isoform A
NM_001275697.1	NP_001262626.1	sulfated, isoform B
NM_001275698.1	NP_001262627.1	sulfated, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Sulf1	NP_001262626.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003374	XP_314283.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sulf1 is required for terminating intestinal stem cell division at the end of regeneration.
Title: Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration.

vertebrate and Drosophila Sulfs have an intrinsically similar activity and the function of Sulfs in the fate of Wnt/Wg ligands is context-dependent
Title: Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation.

DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop.
Title: DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop.

The results demonstrate that sulf1 is a novel Wg target gene and by a feedback mechanism, it negatively regulated Wg signaling and distribution in vivo.
Title: Sulfated is a negative feedback regulator of wingless in Drosophila.

Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation
Title: Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation.

The following Sulf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sulf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07013	NM_080248.3 Latest version!	Drosophila melanogaster sulfated, transcript variant A (Sulf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07013	NM_001275697.1 Latest version!	Drosophila melanogaster sulfated, transcript variant B (Sulf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07013	NM_001275698.1 Latest version!	Drosophila melanogaster sulfated, transcript variant C (Sulf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sulf1 ( NM_080248.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524987.1 Drosophila melanogaster sulfated, transcript variant A (Sulf1), mRNA.

Price & Availability↑

CloneID	OFa07013
Clone ID Related Accession (Same CDS sequence)	NM_080248.3 , NM_001275697.1 , NM_001275698.1
Accession Version	NM_080248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sulfated, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080248.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGTC ACTCCAGTTT GCGCCTTATA ATTGGCGGCC TGATACTCCT CCTCTTTGTG 
CTTAATGTCT TCAGCAAGGA ACAGGGATCT CATTCCCACA AACGCAGCCA TTCGGCGAAG 
AGATTCTCGC GGGACTCCAA CTCCGCACGT GAGCGACGCC CAAACATAAT CCTCATTCTG 
ACCGATGACC AGGATGTGGA GCTGGGCTCC CTTAACTTCA TGCCGCGCAC GTTGCGACTT 
CTTAGAGATG GAGGAGCCGA ATTCCGACAT GCCTATACCA CCACACCCAT GTGCTGTCCG 
GCGAGATCGT CTCTGCTGAC TGGGATGTAC GTCCACAACC ACATGGTGTT TACCAACAAT 
GACAACTGCT CCAGCCCACA GTGGCAGGCC ACTCATGAGA CGCGATCCTA TGCCACATAC 
CTCTCGAATG CAGGCTATCG CACCGGCTAT TTCGGAAAGT ATCTAAACAA GTACAATGGA 
TCCTATATCC CACCAGGTTG GCGGGAGTGG GGAGGATTGA TCATGAACTC CAAGTACTAC 
AACTACAGCA TCAACCTGAA TGGACAAAAA ATTAAGCACG GTTTTGACTA CGCTAAAGAC 
TACTATCCGG ATCTGATAGC CAATGACTCG ATTGCCTTCC TCCGCTCCTC AAAGCAACAG 
AACCAGCGGA AGCCAGTGCT GCTCACCATG AGTTTTCCTG CACCACATGG CCCTGAGGAT 
TCGGCTCCCC AGTATAGTCA TCTCTTCTTT AATGTGACAA CCCATCACAC TCCATCGTAT 
GATCACGCCC CAAATCCGGA CAAGCAATGG ATCCTGAGGG TCACGGAACC CATGCAGCCT 
GTTCACAAAA GGTTCACCAA TCTGCTCATG ACGAAGCGAC TGCAAACGCT CCAAAGTGTC 
GACGTTGCCG TGGAGCGGGT TTATAACGAG CTAAAAGAAC TCGGAGAGCT GGACAACACT 
TATATAGTAT ACACTTCCGA TCATGGTTAT CATCTGGGTC AGTTTGGACT TATTAAAGGA 
AAAAGTTTTC CCTTTGAGTT TGATGTCCGT GTGCCGTTCC TTATCCGTGG TCCAGGGATT 
CAGGCATCTA AGGTGGTCAA TGAGATTGTT TTAAATGTGG ACCTGGCCCC CACTTTCCTG 
GATATGGGTG GTGTGCCAAC GCCTCAGCAT ATGGATGGGC GCAGCATACT GCCACTGCTT 
CTGAGCAGAA ATCGCGCTGT GCGAGACAAC TGGCCAGATA GCTTCCTCAT TGAGAGTTCC 
GGCAGGAGGG AGACAGCTGA GCAGATAGCC GAATCTCGTG CCCGCCTTCA AATTGAAAGG 
CGAAACATGA AACTGGCCAA CAGTTCGCTG CTTGAAGATT TCCTTGAAGG AGCAGGCGAA 
AGTACCACCA TTGTTTCGAG CAGCAGCACC GCTGCCACAT TGATGAGCTC AACCGCTCAG 
CAGCCGGAGG ATGGGGAAGA GGAAGTGGAA ACGGACAACG AAGAGGATGA TGTAGACGGT 
GATGGTGCCA TGGACAGCTC AGCAGCAGCT CTGGAAGAAG ATGACCTCGA CGATGCCGCC 
TTCGAGGAGG GTGATGAAGA GCTTGATCAG GAGTTTCAAC AAAACAACGA TCTTCCACTG 
GCTCCTTATA TAACCAAGAT GATGCGCCTT AACTCGGAGT GCTCTGACCC GGCGCTACTC 
AAAAACTGTC TACCTGGGCA AAAATGGAAG TGCGTCAATG AGGAGGGACG TTGGCGGAAG 
CACAAGTGCA AATTCCATCT TCAGTTAGAG CATCAGTTGG CTGCGATGCC CCGGAAGCAA 
TATCAACGTA ATTGTGCCTG TTTCACTCCC GACGGAGTAG TCTATACCAA GATTCGAGCC 
CCATCTGCTG GATTGCATCG CGTTAACAAG CGCACACATA ACGGCCCAGG AAGGCGTCGA 
AACAAGCGGG AGGTGTTCCA TACTGAGTTA CCTGACGAGA TGGAGGAGCT GTTAGATCTC 
CACCAGGTGG TAGATCAACT TGTTGATCAT ACGCATCGCA GCAAGCGTGA TTTGCCCGCA 
AGCAGCAATG AGACTATTGC CCAGGTCATT CAGCAGATAC AAAGCACCCT GGAGATCTTG 
GAGCTTAAAT TCAACGAGCA CGAGCTACAC GCATCCAACT CTAGTGGCAA CTCATATGAG 
CGGGGAGAAA AGTACACAAA ATCTGGTGGC CATCGGTGCT TCGTGGATGC GACCACGGCT 
AAAGTGAACT GCTCAAATGT GATATACGAC GACGAAAAGA CGTGGCGTAC TTCGCGTACT 
CAAATTGATA TGCTGATCAA GCTTCTAAAG GACAAGATTG GCAAGCTGAA GGAGATGAAG 
AAGCAGTTGA GGGAAAGCAA TAAACAGGCC TTGGCCGCTG GTAGAAGAAA TGATAACCGA 
CGGCGTAATG ATCAAAGTGT GCTGGATAGT GGAGCCGGAC CAGAGTTTAA CATGAGCTAC 
TTTACTGAGA TCAGCAGCAC GCCAAGATCA AACGTCGTAG GTCAAACGGA AGTATTCCAA 
GGATATGGAA GTGCAAGCGC ATTCGATTCA TTGGAGCAAA CGCAGTCGCA TCGGTTTACA 
CCGCGAGCAG AATGCTATTG CGAACCAGAT GTAGGCGAGA ACCATGCAGA CTCTAAGGAA 
ATGGCGCGTG AGGCACGACG AAAGCTTAAA GAGGAGCGAC AGCGCAAGAA GGAACGGAAG 
CGCATTAAGA AGGCTCGTCT GGAAAAGGAG TGTCTGTCCG AGAAGATGAA CTGCTTCTCG 
CACGACAATC AGCACTGGCG AACGGCTCCC CTTTGGAACG ACAGTCCATT TTGCTTCTGT 
ATGAACGCCA ATAATAACAC CTACTCCTGC CTAAGGACCA TCAATGGCAC CCACAACTTT 
TTGTACTGCG AGTTTACCAC AGGCCTTATC ACCTTCTATA ACCTGACCAT TGATCGCTTT 
GAAACGATAA ACCGTGCAGC GGGTCTGACG CCTGGAGAGA GATCCCACAT GCACGATGCT 
TTAGATCAGT TGAAGAGCTG TCGAGGACGC AGTTGCAGCA TCCGGCGACA CCAGAATCAC 
CTGGAGGGCG GATCTAGTGC TCCACTGCTG CCCATCAATC AAGTGCACCG CAACAACAAA 
CGAAAGCACT CTCCTCTCGC GGGCGCAGTT GGCAACTATG CATTCGTAGG CCCACGACTG 
GATATGGAAG CACTGCCTCC GATTAAGCGG CGTAAGCTGT CCAAATACAA CAGATTGACT 
GGCTCGCAGC AGTCGCATAT GAAGCGGCGT CCCTGGAAGC AGACGCCCCT TCAGCAGTCC 
CCCAGATTCT TGCGCACGCA TTCTGTGACA CCAGCCCAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524987.1
CDS	852..4196
Translation	MMRHSSLRLIIGGLILLLFVLNVFSKEQGSHSHKRSHSAKRFSRDSNSARERRPNIILILTDDQDVELGSLNFMPRTLRLLRDGGAEFRHAYTTTPMCCPARSSLLTGMYVHNHMVFTNNDNCSSPQWQATHETRSYATYLSNAGYRTGYFGKYLNKYNGSYIPPGWREWGGLIMNSKYYNYSINLNGQKIKHGFDYAKDYYPDLIANDSIAFLRSSKQQNQRKPVLLTMSFPAPHGPEDSAPQYSHLFFNVTTHHTPSYDHAPNPDKQWILRVTEPMQPVHKRFTNLLMTKRLQTLQSVDVAVERVYNELKELGELDNTYIVYTSDHGYHLGQFGLIKGKSFPFEFDVRVPFLIRGPGIQASKVVNEIVLNVDLAPTFLDMGGVPTPQHMDGRSILPLLLSRNRAVRDNWPDSFLIESSGRRETAEQIAESRARLQIERRNMKLANSSLLEDFLEGAGESTTIVSSSSTAATLMSSTAQQPEDGEEEVETDNEEDDVDGDGAMDSSAAALEEDDLDDAAFEEGDEELDQEFQQNNDLPLAPYITKMMRLNSECSDPALLKNCLPGQKWKCVNEEGRWRKHKCKFHLQLEHQLAAMPRKQYQRNCACFTPDGVVYTKIRAPSAGLHRVNKRTHNGPGRRRNKREVFHTELPDEMEELLDLHQVVDQLVDHTHRSKRDLPASSNETIAQVIQQIQSTLEILELKFNEHELHASNSSGNSYERGEKYTKSGGHRCFVDATTAKVNCSNVIYDDEKTWRTSRTQIDMLIKLLKDKIGKLKEMKKQLRESNKQALAAGRRNDNRRRNDQSVLDSGAGPEFNMSYFTEISSTPRSNVVGQTEVFQGYGSASAFDSLEQTQSHRFTPRAECYCEPDVGENHADSKEMAREARRKLKEERQRKKERKRIKKARLEKECLSEKMNCFSHDNQHWRTAPLWNDSPFCFCMNANNNTYSCLRTINGTHNFLYCEFTTGLITFYNLTIDRFETINRAAGLTPGERSHMHDALDQLKSCRGRSCSIRRHQNHLEGGSSAPLLPINQVHRNNKRKHSPLAGAVGNYAFVGPRLDMEALPPIKRRKLSKYNRLTGSQQSHMKRRPWKQTPLQQSPRFLRTHSVTPAQA

Target ORF information:

RefSeq Version	NM_080248.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sulfated, transcript variant A (Sulf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080248.3

ORF Insert Sequence:

ATGATGCGTC ACTCCAGTTT GCGCCTTATA ATTGGCGGCC TGATACTCCT CCTCTTTGTG 
CTTAATGTCT TCAGCAAGGA ACAGGGATCT CATTCCCACA AACGCAGCCA TTCGGCGAAG 
AGATTCTCGC GGGACTCCAA CTCCGCACGT GAGCGACGCC CAAACATAAT CCTCATTCTG 
ACCGATGACC AGGATGTGGA GCTGGGCTCC CTTAACTTCA TGCCGCGCAC GTTGCGACTT 
CTTAGAGATG GAGGAGCCGA ATTCCGACAT GCCTATACCA CCACACCCAT GTGCTGTCCG 
GCGAGATCGT CTCTGCTGAC TGGGATGTAC GTCCACAACC ACATGGTGTT TACCAACAAT 
GACAACTGCT CCAGCCCACA GTGGCAGGCC ACTCATGAGA CGCGATCCTA TGCCACATAC 
CTCTCGAATG CAGGCTATCG CACCGGCTAT TTCGGAAAGT ATCTAAACAA GTACAATGGA 
TCCTATATCC CACCAGGTTG GCGGGAGTGG GGAGGATTGA TCATGAACTC CAAGTACTAC 
AACTACAGCA TCAACCTGAA TGGACAAAAA ATTAAGCACG GTTTTGACTA CGCTAAAGAC 
TACTATCCGG ATCTGATAGC CAATGACTCG ATTGCCTTCC TCCGCTCCTC AAAGCAACAG 
AACCAGCGGA AGCCAGTGCT GCTCACCATG AGTTTTCCTG CACCACATGG CCCTGAGGAT 
TCGGCTCCCC AGTATAGTCA TCTCTTCTTT AATGTGACAA CCCATCACAC TCCATCGTAT 
GATCACGCCC CAAATCCGGA CAAGCAATGG ATCCTGAGGG TCACGGAACC CATGCAGCCT 
GTTCACAAAA GGTTCACCAA TCTGCTCATG ACGAAGCGAC TGCAAACGCT CCAAAGTGTC 
GACGTTGCCG TGGAGCGGGT TTATAACGAG CTAAAAGAAC TCGGAGAGCT GGACAACACT 
TATATAGTAT ACACTTCCGA TCATGGTTAT CATCTGGGTC AGTTTGGACT TATTAAAGGA 
AAAAGTTTTC CCTTTGAGTT TGATGTCCGT GTGCCGTTCC TTATCCGTGG TCCAGGGATT 
CAGGCATCTA AGGTGGTCAA TGAGATTGTT TTAAATGTGG ACCTGGCCCC CACTTTCCTG 
GATATGGGTG GTGTGCCAAC GCCTCAGCAT ATGGATGGGC GCAGCATACT GCCACTGCTT 
CTGAGCAGAA ATCGCGCTGT GCGAGACAAC TGGCCAGATA GCTTCCTCAT TGAGAGTTCC 
GGCAGGAGGG AGACAGCTGA GCAGATAGCC GAATCTCGTG CCCGCCTTCA AATTGAAAGG 
CGAAACATGA AACTGGCCAA CAGTTCGCTG CTTGAAGATT TCCTTGAAGG AGCAGGCGAA 
AGTACCACCA TTGTTTCGAG CAGCAGCACC GCTGCCACAT TGATGAGCTC AACCGCTCAG 
CAGCCGGAGG ATGGGGAAGA GGAAGTGGAA ACGGACAACG AAGAGGATGA TGTAGACGGT 
GATGGTGCCA TGGACAGCTC AGCAGCAGCT CTGGAAGAAG ATGACCTCGA CGATGCCGCC 
TTCGAGGAGG GTGATGAAGA GCTTGATCAG GAGTTTCAAC AAAACAACGA TCTTCCACTG 
GCTCCTTATA TAACCAAGAT GATGCGCCTT AACTCGGAGT GCTCTGACCC GGCGCTACTC 
AAAAACTGTC TACCTGGGCA AAAATGGAAG TGCGTCAATG AGGAGGGACG TTGGCGGAAG 
CACAAGTGCA AATTCCATCT TCAGTTAGAG CATCAGTTGG CTGCGATGCC CCGGAAGCAA 
TATCAACGTA ATTGTGCCTG TTTCACTCCC GACGGAGTAG TCTATACCAA GATTCGAGCC 
CCATCTGCTG GATTGCATCG CGTTAACAAG CGCACACATA ACGGCCCAGG AAGGCGTCGA 
AACAAGCGGG AGGTGTTCCA TACTGAGTTA CCTGACGAGA TGGAGGAGCT GTTAGATCTC 
CACCAGGTGG TAGATCAACT TGTTGATCAT ACGCATCGCA GCAAGCGTGA TTTGCCCGCA 
AGCAGCAATG AGACTATTGC CCAGGTCATT CAGCAGATAC AAAGCACCCT GGAGATCTTG 
GAGCTTAAAT TCAACGAGCA CGAGCTACAC GCATCCAACT CTAGTGGCAA CTCATATGAG 
CGGGGAGAAA AGTACACAAA ATCTGGTGGC CATCGGTGCT TCGTGGATGC GACCACGGCT 
AAAGTGAACT GCTCAAATGT GATATACGAC GACGAAAAGA CGTGGCGTAC TTCGCGTACT 
CAAATTGATA TGCTGATCAA GCTTCTAAAG GACAAGATTG GCAAGCTGAA GGAGATGAAG 
AAGCAGTTGA GGGAAAGCAA TAAACAGGCC TTGGCCGCTG GTAGAAGAAA TGATAACCGA 
CGGCGTAATG ATCAAAGTGT GCTGGATAGT GGAGCCGGAC CAGAGTTTAA CATGAGCTAC 
TTTACTGAGA TCAGCAGCAC GCCAAGATCA AACGTCGTAG GTCAAACGGA AGTATTCCAA 
GGATATGGAA GTGCAAGCGC ATTCGATTCA TTGGAGCAAA CGCAGTCGCA TCGGTTTACA 
CCGCGAGCAG AATGCTATTG CGAACCAGAT GTAGGCGAGA ACCATGCAGA CTCTAAGGAA 
ATGGCGCGTG AGGCACGACG AAAGCTTAAA GAGGAGCGAC AGCGCAAGAA GGAACGGAAG 
CGCATTAAGA AGGCTCGTCT GGAAAAGGAG TGTCTGTCCG AGAAGATGAA CTGCTTCTCG 
CACGACAATC AGCACTGGCG AACGGCTCCC CTTTGGAACG ACAGTCCATT TTGCTTCTGT 
ATGAACGCCA ATAATAACAC CTACTCCTGC CTAAGGACCA TCAATGGCAC CCACAACTTT 
TTGTACTGCG AGTTTACCAC AGGCCTTATC ACCTTCTATA ACCTGACCAT TGATCGCTTT 
GAAACGATAA ACCGTGCAGC GGGTCTGACG CCTGGAGAGA GATCCCACAT GCACGATGCT 
TTAGATCAGT TGAAGAGCTG TCGAGGACGC AGTTGCAGCA TCCGGCGACA CCAGAATCAC 
CTGGAGGGCG GATCTAGTGC TCCACTGCTG CCCATCAATC AAGTGCACCG CAACAACAAA 
CGAAAGCACT CTCCTCTCGC GGGCGCAGTT GGCAACTATG CATTCGTAGG CCCACGACTG 
GATATGGAAG CACTGCCTCC GATTAAGCGG CGTAAGCTGT CCAAATACAA CAGATTGACT 
GGCTCGCAGC AGTCGCATAT GAAGCGGCGT CCCTGGAAGC AGACGCCCCT TCAGCAGTCC 
CCCAGATTCT TGCGCACGCA TTCTGTGACA CCAGCCCAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sulf1 ( NM_001275697.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262626.1 Drosophila melanogaster sulfated, transcript variant B (Sulf1), mRNA.

Price & Availability↑

CloneID	OFa07013
Clone ID Related Accession (Same CDS sequence)	NM_080248.3 , NM_001275697.1 , NM_001275698.1
Accession Version	NM_001275697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sulfated, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGTC ACTCCAGTTT GCGCCTTATA ATTGGCGGCC TGATACTCCT CCTCTTTGTG 
CTTAATGTCT TCAGCAAGGA ACAGGGATCT CATTCCCACA AACGCAGCCA TTCGGCGAAG 
AGATTCTCGC GGGACTCCAA CTCCGCACGT GAGCGACGCC CAAACATAAT CCTCATTCTG 
ACCGATGACC AGGATGTGGA GCTGGGCTCC CTTAACTTCA TGCCGCGCAC GTTGCGACTT 
CTTAGAGATG GAGGAGCCGA ATTCCGACAT GCCTATACCA CCACACCCAT GTGCTGTCCG 
GCGAGATCGT CTCTGCTGAC TGGGATGTAC GTCCACAACC ACATGGTGTT TACCAACAAT 
GACAACTGCT CCAGCCCACA GTGGCAGGCC ACTCATGAGA CGCGATCCTA TGCCACATAC 
CTCTCGAATG CAGGCTATCG CACCGGCTAT TTCGGAAAGT ATCTAAACAA GTACAATGGA 
TCCTATATCC CACCAGGTTG GCGGGAGTGG GGAGGATTGA TCATGAACTC CAAGTACTAC 
AACTACAGCA TCAACCTGAA TGGACAAAAA ATTAAGCACG GTTTTGACTA CGCTAAAGAC 
TACTATCCGG ATCTGATAGC CAATGACTCG ATTGCCTTCC TCCGCTCCTC AAAGCAACAG 
AACCAGCGGA AGCCAGTGCT GCTCACCATG AGTTTTCCTG CACCACATGG CCCTGAGGAT 
TCGGCTCCCC AGTATAGTCA TCTCTTCTTT AATGTGACAA CCCATCACAC TCCATCGTAT 
GATCACGCCC CAAATCCGGA CAAGCAATGG ATCCTGAGGG TCACGGAACC CATGCAGCCT 
GTTCACAAAA GGTTCACCAA TCTGCTCATG ACGAAGCGAC TGCAAACGCT CCAAAGTGTC 
GACGTTGCCG TGGAGCGGGT TTATAACGAG CTAAAAGAAC TCGGAGAGCT GGACAACACT 
TATATAGTAT ACACTTCCGA TCATGGTTAT CATCTGGGTC AGTTTGGACT TATTAAAGGA 
AAAAGTTTTC CCTTTGAGTT TGATGTCCGT GTGCCGTTCC TTATCCGTGG TCCAGGGATT 
CAGGCATCTA AGGTGGTCAA TGAGATTGTT TTAAATGTGG ACCTGGCCCC CACTTTCCTG 
GATATGGGTG GTGTGCCAAC GCCTCAGCAT ATGGATGGGC GCAGCATACT GCCACTGCTT 
CTGAGCAGAA ATCGCGCTGT GCGAGACAAC TGGCCAGATA GCTTCCTCAT TGAGAGTTCC 
GGCAGGAGGG AGACAGCTGA GCAGATAGCC GAATCTCGTG CCCGCCTTCA AATTGAAAGG 
CGAAACATGA AACTGGCCAA CAGTTCGCTG CTTGAAGATT TCCTTGAAGG AGCAGGCGAA 
AGTACCACCA TTGTTTCGAG CAGCAGCACC GCTGCCACAT TGATGAGCTC AACCGCTCAG 
CAGCCGGAGG ATGGGGAAGA GGAAGTGGAA ACGGACAACG AAGAGGATGA TGTAGACGGT 
GATGGTGCCA TGGACAGCTC AGCAGCAGCT CTGGAAGAAG ATGACCTCGA CGATGCCGCC 
TTCGAGGAGG GTGATGAAGA GCTTGATCAG GAGTTTCAAC AAAACAACGA TCTTCCACTG 
GCTCCTTATA TAACCAAGAT GATGCGCCTT AACTCGGAGT GCTCTGACCC GGCGCTACTC 
AAAAACTGTC TACCTGGGCA AAAATGGAAG TGCGTCAATG AGGAGGGACG TTGGCGGAAG 
CACAAGTGCA AATTCCATCT TCAGTTAGAG CATCAGTTGG CTGCGATGCC CCGGAAGCAA 
TATCAACGTA ATTGTGCCTG TTTCACTCCC GACGGAGTAG TCTATACCAA GATTCGAGCC 
CCATCTGCTG GATTGCATCG CGTTAACAAG CGCACACATA ACGGCCCAGG AAGGCGTCGA 
AACAAGCGGG AGGTGTTCCA TACTGAGTTA CCTGACGAGA TGGAGGAGCT GTTAGATCTC 
CACCAGGTGG TAGATCAACT TGTTGATCAT ACGCATCGCA GCAAGCGTGA TTTGCCCGCA 
AGCAGCAATG AGACTATTGC CCAGGTCATT CAGCAGATAC AAAGCACCCT GGAGATCTTG 
GAGCTTAAAT TCAACGAGCA CGAGCTACAC GCATCCAACT CTAGTGGCAA CTCATATGAG 
CGGGGAGAAA AGTACACAAA ATCTGGTGGC CATCGGTGCT TCGTGGATGC GACCACGGCT 
AAAGTGAACT GCTCAAATGT GATATACGAC GACGAAAAGA CGTGGCGTAC TTCGCGTACT 
CAAATTGATA TGCTGATCAA GCTTCTAAAG GACAAGATTG GCAAGCTGAA GGAGATGAAG 
AAGCAGTTGA GGGAAAGCAA TAAACAGGCC TTGGCCGCTG GTAGAAGAAA TGATAACCGA 
CGGCGTAATG ATCAAAGTGT GCTGGATAGT GGAGCCGGAC CAGAGTTTAA CATGAGCTAC 
TTTACTGAGA TCAGCAGCAC GCCAAGATCA AACGTCGTAG GTCAAACGGA AGTATTCCAA 
GGATATGGAA GTGCAAGCGC ATTCGATTCA TTGGAGCAAA CGCAGTCGCA TCGGTTTACA 
CCGCGAGCAG AATGCTATTG CGAACCAGAT GTAGGCGAGA ACCATGCAGA CTCTAAGGAA 
ATGGCGCGTG AGGCACGACG AAAGCTTAAA GAGGAGCGAC AGCGCAAGAA GGAACGGAAG 
CGCATTAAGA AGGCTCGTCT GGAAAAGGAG TGTCTGTCCG AGAAGATGAA CTGCTTCTCG 
CACGACAATC AGCACTGGCG AACGGCTCCC CTTTGGAACG ACAGTCCATT TTGCTTCTGT 
ATGAACGCCA ATAATAACAC CTACTCCTGC CTAAGGACCA TCAATGGCAC CCACAACTTT 
TTGTACTGCG AGTTTACCAC AGGCCTTATC ACCTTCTATA ACCTGACCAT TGATCGCTTT 
GAAACGATAA ACCGTGCAGC GGGTCTGACG CCTGGAGAGA GATCCCACAT GCACGATGCT 
TTAGATCAGT TGAAGAGCTG TCGAGGACGC AGTTGCAGCA TCCGGCGACA CCAGAATCAC 
CTGGAGGGCG GATCTAGTGC TCCACTGCTG CCCATCAATC AAGTGCACCG CAACAACAAA 
CGAAAGCACT CTCCTCTCGC GGGCGCAGTT GGCAACTATG CATTCGTAGG CCCACGACTG 
GATATGGAAG CACTGCCTCC GATTAAGCGG CGTAAGCTGT CCAAATACAA CAGATTGACT 
GGCTCGCAGC AGTCGCATAT GAAGCGGCGT CCCTGGAAGC AGACGCCCCT TCAGCAGTCC 
CCCAGATTCT TGCGCACGCA TTCTGTGACA CCAGCCCAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262626.1
CDS	1059..4403
Translation	MMRHSSLRLIIGGLILLLFVLNVFSKEQGSHSHKRSHSAKRFSRDSNSARERRPNIILILTDDQDVELGSLNFMPRTLRLLRDGGAEFRHAYTTTPMCCPARSSLLTGMYVHNHMVFTNNDNCSSPQWQATHETRSYATYLSNAGYRTGYFGKYLNKYNGSYIPPGWREWGGLIMNSKYYNYSINLNGQKIKHGFDYAKDYYPDLIANDSIAFLRSSKQQNQRKPVLLTMSFPAPHGPEDSAPQYSHLFFNVTTHHTPSYDHAPNPDKQWILRVTEPMQPVHKRFTNLLMTKRLQTLQSVDVAVERVYNELKELGELDNTYIVYTSDHGYHLGQFGLIKGKSFPFEFDVRVPFLIRGPGIQASKVVNEIVLNVDLAPTFLDMGGVPTPQHMDGRSILPLLLSRNRAVRDNWPDSFLIESSGRRETAEQIAESRARLQIERRNMKLANSSLLEDFLEGAGESTTIVSSSSTAATLMSSTAQQPEDGEEEVETDNEEDDVDGDGAMDSSAAALEEDDLDDAAFEEGDEELDQEFQQNNDLPLAPYITKMMRLNSECSDPALLKNCLPGQKWKCVNEEGRWRKHKCKFHLQLEHQLAAMPRKQYQRNCACFTPDGVVYTKIRAPSAGLHRVNKRTHNGPGRRRNKREVFHTELPDEMEELLDLHQVVDQLVDHTHRSKRDLPASSNETIAQVIQQIQSTLEILELKFNEHELHASNSSGNSYERGEKYTKSGGHRCFVDATTAKVNCSNVIYDDEKTWRTSRTQIDMLIKLLKDKIGKLKEMKKQLRESNKQALAAGRRNDNRRRNDQSVLDSGAGPEFNMSYFTEISSTPRSNVVGQTEVFQGYGSASAFDSLEQTQSHRFTPRAECYCEPDVGENHADSKEMAREARRKLKEERQRKKERKRIKKARLEKECLSEKMNCFSHDNQHWRTAPLWNDSPFCFCMNANNNTYSCLRTINGTHNFLYCEFTTGLITFYNLTIDRFETINRAAGLTPGERSHMHDALDQLKSCRGRSCSIRRHQNHLEGGSSAPLLPINQVHRNNKRKHSPLAGAVGNYAFVGPRLDMEALPPIKRRKLSKYNRLTGSQQSHMKRRPWKQTPLQQSPRFLRTHSVTPAQA

Target ORF information:

RefSeq Version	NM_001275697.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sulfated, transcript variant B (Sulf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275697.1

ORF Insert Sequence:

ATGATGCGTC ACTCCAGTTT GCGCCTTATA ATTGGCGGCC TGATACTCCT CCTCTTTGTG 
CTTAATGTCT TCAGCAAGGA ACAGGGATCT CATTCCCACA AACGCAGCCA TTCGGCGAAG 
AGATTCTCGC GGGACTCCAA CTCCGCACGT GAGCGACGCC CAAACATAAT CCTCATTCTG 
ACCGATGACC AGGATGTGGA GCTGGGCTCC CTTAACTTCA TGCCGCGCAC GTTGCGACTT 
CTTAGAGATG GAGGAGCCGA ATTCCGACAT GCCTATACCA CCACACCCAT GTGCTGTCCG 
GCGAGATCGT CTCTGCTGAC TGGGATGTAC GTCCACAACC ACATGGTGTT TACCAACAAT 
GACAACTGCT CCAGCCCACA GTGGCAGGCC ACTCATGAGA CGCGATCCTA TGCCACATAC 
CTCTCGAATG CAGGCTATCG CACCGGCTAT TTCGGAAAGT ATCTAAACAA GTACAATGGA 
TCCTATATCC CACCAGGTTG GCGGGAGTGG GGAGGATTGA TCATGAACTC CAAGTACTAC 
AACTACAGCA TCAACCTGAA TGGACAAAAA ATTAAGCACG GTTTTGACTA CGCTAAAGAC 
TACTATCCGG ATCTGATAGC CAATGACTCG ATTGCCTTCC TCCGCTCCTC AAAGCAACAG 
AACCAGCGGA AGCCAGTGCT GCTCACCATG AGTTTTCCTG CACCACATGG CCCTGAGGAT 
TCGGCTCCCC AGTATAGTCA TCTCTTCTTT AATGTGACAA CCCATCACAC TCCATCGTAT 
GATCACGCCC CAAATCCGGA CAAGCAATGG ATCCTGAGGG TCACGGAACC CATGCAGCCT 
GTTCACAAAA GGTTCACCAA TCTGCTCATG ACGAAGCGAC TGCAAACGCT CCAAAGTGTC 
GACGTTGCCG TGGAGCGGGT TTATAACGAG CTAAAAGAAC TCGGAGAGCT GGACAACACT 
TATATAGTAT ACACTTCCGA TCATGGTTAT CATCTGGGTC AGTTTGGACT TATTAAAGGA 
AAAAGTTTTC CCTTTGAGTT TGATGTCCGT GTGCCGTTCC TTATCCGTGG TCCAGGGATT 
CAGGCATCTA AGGTGGTCAA TGAGATTGTT TTAAATGTGG ACCTGGCCCC CACTTTCCTG 
GATATGGGTG GTGTGCCAAC GCCTCAGCAT ATGGATGGGC GCAGCATACT GCCACTGCTT 
CTGAGCAGAA ATCGCGCTGT GCGAGACAAC TGGCCAGATA GCTTCCTCAT TGAGAGTTCC 
GGCAGGAGGG AGACAGCTGA GCAGATAGCC GAATCTCGTG CCCGCCTTCA AATTGAAAGG 
CGAAACATGA AACTGGCCAA CAGTTCGCTG CTTGAAGATT TCCTTGAAGG AGCAGGCGAA 
AGTACCACCA TTGTTTCGAG CAGCAGCACC GCTGCCACAT TGATGAGCTC AACCGCTCAG 
CAGCCGGAGG ATGGGGAAGA GGAAGTGGAA ACGGACAACG AAGAGGATGA TGTAGACGGT 
GATGGTGCCA TGGACAGCTC AGCAGCAGCT CTGGAAGAAG ATGACCTCGA CGATGCCGCC 
TTCGAGGAGG GTGATGAAGA GCTTGATCAG GAGTTTCAAC AAAACAACGA TCTTCCACTG 
GCTCCTTATA TAACCAAGAT GATGCGCCTT AACTCGGAGT GCTCTGACCC GGCGCTACTC 
AAAAACTGTC TACCTGGGCA AAAATGGAAG TGCGTCAATG AGGAGGGACG TTGGCGGAAG 
CACAAGTGCA AATTCCATCT TCAGTTAGAG CATCAGTTGG CTGCGATGCC CCGGAAGCAA 
TATCAACGTA ATTGTGCCTG TTTCACTCCC GACGGAGTAG TCTATACCAA GATTCGAGCC 
CCATCTGCTG GATTGCATCG CGTTAACAAG CGCACACATA ACGGCCCAGG AAGGCGTCGA 
AACAAGCGGG AGGTGTTCCA TACTGAGTTA CCTGACGAGA TGGAGGAGCT GTTAGATCTC 
CACCAGGTGG TAGATCAACT TGTTGATCAT ACGCATCGCA GCAAGCGTGA TTTGCCCGCA 
AGCAGCAATG AGACTATTGC CCAGGTCATT CAGCAGATAC AAAGCACCCT GGAGATCTTG 
GAGCTTAAAT TCAACGAGCA CGAGCTACAC GCATCCAACT CTAGTGGCAA CTCATATGAG 
CGGGGAGAAA AGTACACAAA ATCTGGTGGC CATCGGTGCT TCGTGGATGC GACCACGGCT 
AAAGTGAACT GCTCAAATGT GATATACGAC GACGAAAAGA CGTGGCGTAC TTCGCGTACT 
CAAATTGATA TGCTGATCAA GCTTCTAAAG GACAAGATTG GCAAGCTGAA GGAGATGAAG 
AAGCAGTTGA GGGAAAGCAA TAAACAGGCC TTGGCCGCTG GTAGAAGAAA TGATAACCGA 
CGGCGTAATG ATCAAAGTGT GCTGGATAGT GGAGCCGGAC CAGAGTTTAA CATGAGCTAC 
TTTACTGAGA TCAGCAGCAC GCCAAGATCA AACGTCGTAG GTCAAACGGA AGTATTCCAA 
GGATATGGAA GTGCAAGCGC ATTCGATTCA TTGGAGCAAA CGCAGTCGCA TCGGTTTACA 
CCGCGAGCAG AATGCTATTG CGAACCAGAT GTAGGCGAGA ACCATGCAGA CTCTAAGGAA 
ATGGCGCGTG AGGCACGACG AAAGCTTAAA GAGGAGCGAC AGCGCAAGAA GGAACGGAAG 
CGCATTAAGA AGGCTCGTCT GGAAAAGGAG TGTCTGTCCG AGAAGATGAA CTGCTTCTCG 
CACGACAATC AGCACTGGCG AACGGCTCCC CTTTGGAACG ACAGTCCATT TTGCTTCTGT 
ATGAACGCCA ATAATAACAC CTACTCCTGC CTAAGGACCA TCAATGGCAC CCACAACTTT 
TTGTACTGCG AGTTTACCAC AGGCCTTATC ACCTTCTATA ACCTGACCAT TGATCGCTTT 
GAAACGATAA ACCGTGCAGC GGGTCTGACG CCTGGAGAGA GATCCCACAT GCACGATGCT 
TTAGATCAGT TGAAGAGCTG TCGAGGACGC AGTTGCAGCA TCCGGCGACA CCAGAATCAC 
CTGGAGGGCG GATCTAGTGC TCCACTGCTG CCCATCAATC AAGTGCACCG CAACAACAAA 
CGAAAGCACT CTCCTCTCGC GGGCGCAGTT GGCAACTATG CATTCGTAGG CCCACGACTG 
GATATGGAAG CACTGCCTCC GATTAAGCGG CGTAAGCTGT CCAAATACAA CAGATTGACT 
GGCTCGCAGC AGTCGCATAT GAAGCGGCGT CCCTGGAAGC AGACGCCCCT TCAGCAGTCC 
CCCAGATTCT TGCGCACGCA TTCTGTGACA CCAGCCCAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sulf1 ( NM_001275698.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262627.1 Drosophila melanogaster sulfated, transcript variant C (Sulf1), mRNA.

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CloneID	OFa07013
Clone ID Related Accession (Same CDS sequence)	NM_080248.3 , NM_001275697.1 , NM_001275698.1
Accession Version	NM_001275698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sulfated, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCGTC ACTCCAGTTT GCGCCTTATA ATTGGCGGCC TGATACTCCT CCTCTTTGTG 
CTTAATGTCT TCAGCAAGGA ACAGGGATCT CATTCCCACA AACGCAGCCA TTCGGCGAAG 
AGATTCTCGC GGGACTCCAA CTCCGCACGT GAGCGACGCC CAAACATAAT CCTCATTCTG 
ACCGATGACC AGGATGTGGA GCTGGGCTCC CTTAACTTCA TGCCGCGCAC GTTGCGACTT 
CTTAGAGATG GAGGAGCCGA ATTCCGACAT GCCTATACCA CCACACCCAT GTGCTGTCCG 
GCGAGATCGT CTCTGCTGAC TGGGATGTAC GTCCACAACC ACATGGTGTT TACCAACAAT 
GACAACTGCT CCAGCCCACA GTGGCAGGCC ACTCATGAGA CGCGATCCTA TGCCACATAC 
CTCTCGAATG CAGGCTATCG CACCGGCTAT TTCGGAAAGT ATCTAAACAA GTACAATGGA 
TCCTATATCC CACCAGGTTG GCGGGAGTGG GGAGGATTGA TCATGAACTC CAAGTACTAC 
AACTACAGCA TCAACCTGAA TGGACAAAAA ATTAAGCACG GTTTTGACTA CGCTAAAGAC 
TACTATCCGG ATCTGATAGC CAATGACTCG ATTGCCTTCC TCCGCTCCTC AAAGCAACAG 
AACCAGCGGA AGCCAGTGCT GCTCACCATG AGTTTTCCTG CACCACATGG CCCTGAGGAT 
TCGGCTCCCC AGTATAGTCA TCTCTTCTTT AATGTGACAA CCCATCACAC TCCATCGTAT 
GATCACGCCC CAAATCCGGA CAAGCAATGG ATCCTGAGGG TCACGGAACC CATGCAGCCT 
GTTCACAAAA GGTTCACCAA TCTGCTCATG ACGAAGCGAC TGCAAACGCT CCAAAGTGTC 
GACGTTGCCG TGGAGCGGGT TTATAACGAG CTAAAAGAAC TCGGAGAGCT GGACAACACT 
TATATAGTAT ACACTTCCGA TCATGGTTAT CATCTGGGTC AGTTTGGACT TATTAAAGGA 
AAAAGTTTTC CCTTTGAGTT TGATGTCCGT GTGCCGTTCC TTATCCGTGG TCCAGGGATT 
CAGGCATCTA AGGTGGTCAA TGAGATTGTT TTAAATGTGG ACCTGGCCCC CACTTTCCTG 
GATATGGGTG GTGTGCCAAC GCCTCAGCAT ATGGATGGGC GCAGCATACT GCCACTGCTT 
CTGAGCAGAA ATCGCGCTGT GCGAGACAAC TGGCCAGATA GCTTCCTCAT TGAGAGTTCC 
GGCAGGAGGG AGACAGCTGA GCAGATAGCC GAATCTCGTG CCCGCCTTCA AATTGAAAGG 
CGAAACATGA AACTGGCCAA CAGTTCGCTG CTTGAAGATT TCCTTGAAGG AGCAGGCGAA 
AGTACCACCA TTGTTTCGAG CAGCAGCACC GCTGCCACAT TGATGAGCTC AACCGCTCAG 
CAGCCGGAGG ATGGGGAAGA GGAAGTGGAA ACGGACAACG AAGAGGATGA TGTAGACGGT 
GATGGTGCCA TGGACAGCTC AGCAGCAGCT CTGGAAGAAG ATGACCTCGA CGATGCCGCC 
TTCGAGGAGG GTGATGAAGA GCTTGATCAG GAGTTTCAAC AAAACAACGA TCTTCCACTG 
GCTCCTTATA TAACCAAGAT GATGCGCCTT AACTCGGAGT GCTCTGACCC GGCGCTACTC 
AAAAACTGTC TACCTGGGCA AAAATGGAAG TGCGTCAATG AGGAGGGACG TTGGCGGAAG 
CACAAGTGCA AATTCCATCT TCAGTTAGAG CATCAGTTGG CTGCGATGCC CCGGAAGCAA 
TATCAACGTA ATTGTGCCTG TTTCACTCCC GACGGAGTAG TCTATACCAA GATTCGAGCC 
CCATCTGCTG GATTGCATCG CGTTAACAAG CGCACACATA ACGGCCCAGG AAGGCGTCGA 
AACAAGCGGG AGGTGTTCCA TACTGAGTTA CCTGACGAGA TGGAGGAGCT GTTAGATCTC 
CACCAGGTGG TAGATCAACT TGTTGATCAT ACGCATCGCA GCAAGCGTGA TTTGCCCGCA 
AGCAGCAATG AGACTATTGC CCAGGTCATT CAGCAGATAC AAAGCACCCT GGAGATCTTG 
GAGCTTAAAT TCAACGAGCA CGAGCTACAC GCATCCAACT CTAGTGGCAA CTCATATGAG 
CGGGGAGAAA AGTACACAAA ATCTGGTGGC CATCGGTGCT TCGTGGATGC GACCACGGCT 
AAAGTGAACT GCTCAAATGT GATATACGAC GACGAAAAGA CGTGGCGTAC TTCGCGTACT 
CAAATTGATA TGCTGATCAA GCTTCTAAAG GACAAGATTG GCAAGCTGAA GGAGATGAAG 
AAGCAGTTGA GGGAAAGCAA TAAACAGGCC TTGGCCGCTG GTAGAAGAAA TGATAACCGA 
CGGCGTAATG ATCAAAGTGT GCTGGATAGT GGAGCCGGAC CAGAGTTTAA CATGAGCTAC 
TTTACTGAGA TCAGCAGCAC GCCAAGATCA AACGTCGTAG GTCAAACGGA AGTATTCCAA 
GGATATGGAA GTGCAAGCGC ATTCGATTCA TTGGAGCAAA CGCAGTCGCA TCGGTTTACA 
CCGCGAGCAG AATGCTATTG CGAACCAGAT GTAGGCGAGA ACCATGCAGA CTCTAAGGAA 
ATGGCGCGTG AGGCACGACG AAAGCTTAAA GAGGAGCGAC AGCGCAAGAA GGAACGGAAG 
CGCATTAAGA AGGCTCGTCT GGAAAAGGAG TGTCTGTCCG AGAAGATGAA CTGCTTCTCG 
CACGACAATC AGCACTGGCG AACGGCTCCC CTTTGGAACG ACAGTCCATT TTGCTTCTGT 
ATGAACGCCA ATAATAACAC CTACTCCTGC CTAAGGACCA TCAATGGCAC CCACAACTTT 
TTGTACTGCG AGTTTACCAC AGGCCTTATC ACCTTCTATA ACCTGACCAT TGATCGCTTT 
GAAACGATAA ACCGTGCAGC GGGTCTGACG CCTGGAGAGA GATCCCACAT GCACGATGCT 
TTAGATCAGT TGAAGAGCTG TCGAGGACGC AGTTGCAGCA TCCGGCGACA CCAGAATCAC 
CTGGAGGGCG GATCTAGTGC TCCACTGCTG CCCATCAATC AAGTGCACCG CAACAACAAA 
CGAAAGCACT CTCCTCTCGC GGGCGCAGTT GGCAACTATG CATTCGTAGG CCCACGACTG 
GATATGGAAG CACTGCCTCC GATTAAGCGG CGTAAGCTGT CCAAATACAA CAGATTGACT 
GGCTCGCAGC AGTCGCATAT GAAGCGGCGT CCCTGGAAGC AGACGCCCCT TCAGCAGTCC 
CCCAGATTCT TGCGCACGCA TTCTGTGACA CCAGCCCAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262627.1
CDS	418..3762
Translation	MMRHSSLRLIIGGLILLLFVLNVFSKEQGSHSHKRSHSAKRFSRDSNSARERRPNIILILTDDQDVELGSLNFMPRTLRLLRDGGAEFRHAYTTTPMCCPARSSLLTGMYVHNHMVFTNNDNCSSPQWQATHETRSYATYLSNAGYRTGYFGKYLNKYNGSYIPPGWREWGGLIMNSKYYNYSINLNGQKIKHGFDYAKDYYPDLIANDSIAFLRSSKQQNQRKPVLLTMSFPAPHGPEDSAPQYSHLFFNVTTHHTPSYDHAPNPDKQWILRVTEPMQPVHKRFTNLLMTKRLQTLQSVDVAVERVYNELKELGELDNTYIVYTSDHGYHLGQFGLIKGKSFPFEFDVRVPFLIRGPGIQASKVVNEIVLNVDLAPTFLDMGGVPTPQHMDGRSILPLLLSRNRAVRDNWPDSFLIESSGRRETAEQIAESRARLQIERRNMKLANSSLLEDFLEGAGESTTIVSSSSTAATLMSSTAQQPEDGEEEVETDNEEDDVDGDGAMDSSAAALEEDDLDDAAFEEGDEELDQEFQQNNDLPLAPYITKMMRLNSECSDPALLKNCLPGQKWKCVNEEGRWRKHKCKFHLQLEHQLAAMPRKQYQRNCACFTPDGVVYTKIRAPSAGLHRVNKRTHNGPGRRRNKREVFHTELPDEMEELLDLHQVVDQLVDHTHRSKRDLPASSNETIAQVIQQIQSTLEILELKFNEHELHASNSSGNSYERGEKYTKSGGHRCFVDATTAKVNCSNVIYDDEKTWRTSRTQIDMLIKLLKDKIGKLKEMKKQLRESNKQALAAGRRNDNRRRNDQSVLDSGAGPEFNMSYFTEISSTPRSNVVGQTEVFQGYGSASAFDSLEQTQSHRFTPRAECYCEPDVGENHADSKEMAREARRKLKEERQRKKERKRIKKARLEKECLSEKMNCFSHDNQHWRTAPLWNDSPFCFCMNANNNTYSCLRTINGTHNFLYCEFTTGLITFYNLTIDRFETINRAAGLTPGERSHMHDALDQLKSCRGRSCSIRRHQNHLEGGSSAPLLPINQVHRNNKRKHSPLAGAVGNYAFVGPRLDMEALPPIKRRKLSKYNRLTGSQQSHMKRRPWKQTPLQQSPRFLRTHSVTPAQA

Target ORF information:

RefSeq Version	NM_001275698.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sulfated, transcript variant C (Sulf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275698.1

ORF Insert Sequence:

ATGATGCGTC ACTCCAGTTT GCGCCTTATA ATTGGCGGCC TGATACTCCT CCTCTTTGTG 
CTTAATGTCT TCAGCAAGGA ACAGGGATCT CATTCCCACA AACGCAGCCA TTCGGCGAAG 
AGATTCTCGC GGGACTCCAA CTCCGCACGT GAGCGACGCC CAAACATAAT CCTCATTCTG 
ACCGATGACC AGGATGTGGA GCTGGGCTCC CTTAACTTCA TGCCGCGCAC GTTGCGACTT 
CTTAGAGATG GAGGAGCCGA ATTCCGACAT GCCTATACCA CCACACCCAT GTGCTGTCCG 
GCGAGATCGT CTCTGCTGAC TGGGATGTAC GTCCACAACC ACATGGTGTT TACCAACAAT 
GACAACTGCT CCAGCCCACA GTGGCAGGCC ACTCATGAGA CGCGATCCTA TGCCACATAC 
CTCTCGAATG CAGGCTATCG CACCGGCTAT TTCGGAAAGT ATCTAAACAA GTACAATGGA 
TCCTATATCC CACCAGGTTG GCGGGAGTGG GGAGGATTGA TCATGAACTC CAAGTACTAC 
AACTACAGCA TCAACCTGAA TGGACAAAAA ATTAAGCACG GTTTTGACTA CGCTAAAGAC 
TACTATCCGG ATCTGATAGC CAATGACTCG ATTGCCTTCC TCCGCTCCTC AAAGCAACAG 
AACCAGCGGA AGCCAGTGCT GCTCACCATG AGTTTTCCTG CACCACATGG CCCTGAGGAT 
TCGGCTCCCC AGTATAGTCA TCTCTTCTTT AATGTGACAA CCCATCACAC TCCATCGTAT 
GATCACGCCC CAAATCCGGA CAAGCAATGG ATCCTGAGGG TCACGGAACC CATGCAGCCT 
GTTCACAAAA GGTTCACCAA TCTGCTCATG ACGAAGCGAC TGCAAACGCT CCAAAGTGTC 
GACGTTGCCG TGGAGCGGGT TTATAACGAG CTAAAAGAAC TCGGAGAGCT GGACAACACT 
TATATAGTAT ACACTTCCGA TCATGGTTAT CATCTGGGTC AGTTTGGACT TATTAAAGGA 
AAAAGTTTTC CCTTTGAGTT TGATGTCCGT GTGCCGTTCC TTATCCGTGG TCCAGGGATT 
CAGGCATCTA AGGTGGTCAA TGAGATTGTT TTAAATGTGG ACCTGGCCCC CACTTTCCTG 
GATATGGGTG GTGTGCCAAC GCCTCAGCAT ATGGATGGGC GCAGCATACT GCCACTGCTT 
CTGAGCAGAA ATCGCGCTGT GCGAGACAAC TGGCCAGATA GCTTCCTCAT TGAGAGTTCC 
GGCAGGAGGG AGACAGCTGA GCAGATAGCC GAATCTCGTG CCCGCCTTCA AATTGAAAGG 
CGAAACATGA AACTGGCCAA CAGTTCGCTG CTTGAAGATT TCCTTGAAGG AGCAGGCGAA 
AGTACCACCA TTGTTTCGAG CAGCAGCACC GCTGCCACAT TGATGAGCTC AACCGCTCAG 
CAGCCGGAGG ATGGGGAAGA GGAAGTGGAA ACGGACAACG AAGAGGATGA TGTAGACGGT 
GATGGTGCCA TGGACAGCTC AGCAGCAGCT CTGGAAGAAG ATGACCTCGA CGATGCCGCC 
TTCGAGGAGG GTGATGAAGA GCTTGATCAG GAGTTTCAAC AAAACAACGA TCTTCCACTG 
GCTCCTTATA TAACCAAGAT GATGCGCCTT AACTCGGAGT GCTCTGACCC GGCGCTACTC 
AAAAACTGTC TACCTGGGCA AAAATGGAAG TGCGTCAATG AGGAGGGACG TTGGCGGAAG 
CACAAGTGCA AATTCCATCT TCAGTTAGAG CATCAGTTGG CTGCGATGCC CCGGAAGCAA 
TATCAACGTA ATTGTGCCTG TTTCACTCCC GACGGAGTAG TCTATACCAA GATTCGAGCC 
CCATCTGCTG GATTGCATCG CGTTAACAAG CGCACACATA ACGGCCCAGG AAGGCGTCGA 
AACAAGCGGG AGGTGTTCCA TACTGAGTTA CCTGACGAGA TGGAGGAGCT GTTAGATCTC 
CACCAGGTGG TAGATCAACT TGTTGATCAT ACGCATCGCA GCAAGCGTGA TTTGCCCGCA 
AGCAGCAATG AGACTATTGC CCAGGTCATT CAGCAGATAC AAAGCACCCT GGAGATCTTG 
GAGCTTAAAT TCAACGAGCA CGAGCTACAC GCATCCAACT CTAGTGGCAA CTCATATGAG 
CGGGGAGAAA AGTACACAAA ATCTGGTGGC CATCGGTGCT TCGTGGATGC GACCACGGCT 
AAAGTGAACT GCTCAAATGT GATATACGAC GACGAAAAGA CGTGGCGTAC TTCGCGTACT 
CAAATTGATA TGCTGATCAA GCTTCTAAAG GACAAGATTG GCAAGCTGAA GGAGATGAAG 
AAGCAGTTGA GGGAAAGCAA TAAACAGGCC TTGGCCGCTG GTAGAAGAAA TGATAACCGA 
CGGCGTAATG ATCAAAGTGT GCTGGATAGT GGAGCCGGAC CAGAGTTTAA CATGAGCTAC 
TTTACTGAGA TCAGCAGCAC GCCAAGATCA AACGTCGTAG GTCAAACGGA AGTATTCCAA 
GGATATGGAA GTGCAAGCGC ATTCGATTCA TTGGAGCAAA CGCAGTCGCA TCGGTTTACA 
CCGCGAGCAG AATGCTATTG CGAACCAGAT GTAGGCGAGA ACCATGCAGA CTCTAAGGAA 
ATGGCGCGTG AGGCACGACG AAAGCTTAAA GAGGAGCGAC AGCGCAAGAA GGAACGGAAG 
CGCATTAAGA AGGCTCGTCT GGAAAAGGAG TGTCTGTCCG AGAAGATGAA CTGCTTCTCG 
CACGACAATC AGCACTGGCG AACGGCTCCC CTTTGGAACG ACAGTCCATT TTGCTTCTGT 
ATGAACGCCA ATAATAACAC CTACTCCTGC CTAAGGACCA TCAATGGCAC CCACAACTTT 
TTGTACTGCG AGTTTACCAC AGGCCTTATC ACCTTCTATA ACCTGACCAT TGATCGCTTT 
GAAACGATAA ACCGTGCAGC GGGTCTGACG CCTGGAGAGA GATCCCACAT GCACGATGCT 
TTAGATCAGT TGAAGAGCTG TCGAGGACGC AGTTGCAGCA TCCGGCGACA CCAGAATCAC 
CTGGAGGGCG GATCTAGTGC TCCACTGCTG CCCATCAATC AAGTGCACCG CAACAACAAA 
CGAAAGCACT CTCCTCTCGC GGGCGCAGTT GGCAACTATG CATTCGTAGG CCCACGACTG 
GATATGGAAG CACTGCCTCC GATTAAGCGG CGTAAGCTGT CCAAATACAA CAGATTGACT 
GGCTCGCAGC AGTCGCATAT GAAGCGGCGT CCCTGGAAGC AGACGCCCCT TCAGCAGTCC 
CCCAGATTCT TGCGCACGCA TTCTGTGACA CCAGCCCAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sulf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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