Grip cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grip
Entrez Gene ID	50391
Full Name	Glutamate receptor binding protein
Synonyms	CG14447,CG5980,DGrip,Dgrip,Dmel\CG14447,GRIP,dGRIP,dgrip,grip
General protein information	Preferred Names Glutamate receptor binding protein Names CG14447 gene product from transcript CG14447-RA CG14447-PA CG14447-PB CG14447-PC Glutamate-receptor-interacting-protein Grip-PA Grip-PB Grip-PC glutamate receptor binding protein glutamate receptor interacting protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5D1-5D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_132057.3	NP_572285.2	glutamate receptor binding protein, isoform A
NM_001297992.1	NP_001284921.1	glutamate receptor binding protein, isoform C
NM_001297991.1	NP_001284920.1	glutamate receptor binding protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

propose that Perd regulates projection of myotube processes the myotendinous junction by priming formation of a protein complex through its intracellular interaction with Grip and the tendon cell-expressed laminin-binding alpha PS1-beta PS integrin
Title: The transmembrane protein Perdido interacts with Grip and integrins to mediate myotube projection and attachment in the Drosophila embryo.

Kon is enriched at myotube tips during targeting and signals through the intracellular adaptor Dgrip in a conserved molecular pathway.
Title: The transmembrane protein Kon-tiki couples to Dgrip to mediate myotube targeting in Drosophila.

We propose that Ed and DGrip form a signaling complex, where competition between N-terminal and the C-terminal PDZDs of DGrip for Ed binding controls signaling function
Title: Complex interaction of Drosophila GRIP PDZ domains and Echinoid during muscle morphogenesis.

Data show that GRIP is necessary for the trafficking of Frizzled-2 to the nucleus.
Title: Nuclear trafficking of Drosophila Frizzled-2 during synapse development requires the PDZ protein dGRIP.

The following Grip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02447	NM_132057.3 Latest version!	Drosophila melanogaster glutamate receptor binding protein, transcript variant A (Grip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa22285	NM_001297992.1 Latest version!	Drosophila melanogaster glutamate receptor binding protein, transcript variant C (Grip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa22285	NM_001297991.1 Latest version!	Drosophila melanogaster glutamate receptor binding protein, transcript variant B (Grip), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grip ( NM_132057.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572285.2 Drosophila melanogaster glutamate receptor binding protein, transcript variant A (Grip), mRNA.

Price & Availability↑

CloneID	OFa02447
Clone ID Related Accession (Same CDS sequence)	NM_132057.3
Accession Version	NM_132057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glutamate receptor binding protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132057.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGT GGAAATCGAA GAAGCCCATT GGTGGCTGTG TGCCCGGAAA ATCGGCCGCC 
CTAAAACAGG ACCAACAGCA GCAACAACAA CAGCAACAGG AGTCGCACAA CAACTCCTTC 
AATGGACACC ATCATCCGCC GACGGACACT GCCTTGGCGC CCATGTTATC CGTTGACCGG 
GCGATGAGTC CGGCGCAATC GGAGGACTCC GGATTGGCAC CAGAAAGGGG TACCACCTAC 
GCCACCATCA CTCTGCCACG GAATGCCCTG CATTTGGCCA TCACATTCGC AGAACGCAAC 
GATCTGTCGT ATCCACCGGT GGTGGGCTCC CTAAATCCGG TGGGTCATGC GGCGGACTTC 
CTGGCGCCCG GTGATCGTCT CCACCAGATC GACGGCATAT CGACAATTGG TTTGAGCAAC 
CAGAAGGTGA TGAACATGCT CTGCGCTGGA GGATCGGATG CGTGTCCAGC CATCGTGGAG 
ATCGAGTACT CTCTGCCCGA ATGCACAGTT TCCCAGAACA GCCTTTGTGT GACCTCGAAG 
CTGGCGCAGA TCACCGTGGA GCGGGAGAGC GGATGCCTGG GCCTGACGTT GAGGGGCGGT 
GCCGACTACC CGCTGATCGT CACCCATGTG AGGCCCCATG GACCAGTGTA TAAGACCGGT 
CGCATCAAGC CAGGCGATCG GTTGCTGCGC GTCGATAATG TCTCACTGAT TGGCAAGACG 
CTGGCGGAGG CGCAGCAGAT CATCAAGTGC GGCGGTCATG TTTCCGGCTA CACCAACCTG 
ACCATCGAGT ACGATGTTTC TGTGGTGCAG AGCGTTGAGT TCTCGATGGG ACCGCTGCTC 
ATCGAGATCG AGCGACCGAT GAACGACAAG TTGGGCCTGG TGCTGTGCAA TTATACGGCG 
GCTGTGCCCG CTTCCGGTTC CGGTTCCAGT ACTCCTTCCA GCGGGGAGAA GATCGAGGAA 
TCGGCAGGCG TCTTTATCGC CAGTATCCTG CCCGCCAGCA TTGCAGATCG TTGCGGCGCC 
TTATCCGTTG GCGATCAGGT GCTCTCCATC GACGACACAA TGATCGAGCA TACCGCCTTC 
AGTCCCGACG AGGTGATGAC CATTTTGGAC ACCAGCACGG GTCGCGGCTA CACGCAGATG 
CAGATCATGC CCGCTCACGC GCTGGCACGT CGCGGTCATA CAACCTTGGG TAGTCCCAAG 
TACAGCTTTA GCACACTGGA GTCCCGTAAA TCCTCGACGG CGGGTCGCCA GCGTCAGCGT 
TTCGCGAGGA AAAGTTCCCT ACCGTTGGAG AATCCTGCAT GCGGCACTCC AGGCTCCACG 
GGAGGATCGA TGGGTACTGG TGGCATCCAT GGCAGCAGCA GCTTGGGCAT GGGTCTGGGC 
CTCTGCCGGG CGGAGAGCTT TCCCGTTCTC CTCGATTGCA GCCATGGAGC GGGCATTATC 
TTGTCTGAAA CGGTATCCGG ATCTGGAGGA TCGGGTGCTG TAGCCATTGC TCAGATCGTG 
CCGGATTCGG TGGCCGATCG CAGTGGCTGC ATTCAGGCGG GAGATCGCAT CGTGGCCATT 
AACAAGATGT ACAGCTTGGA TGCGGCGGCC ATGAGGCAAC TGCTCGAAGG AGGATCCAGT 
CGCAATGGAG CAAGCAATGG AACGCCGGCC AACTGGCTAG AACTGGAGAT CGAGTTCGAT 
ATGCCGGATG CCGTGGTGCC CGCCAGTGGT GTCTTTAGTG TCAAATTGCT CAGGGCCGGC 
AAATGTGGAC TGGGTCTGAG TGTGAGTGGT TCCAGTCATG GTGGTCTGGT CATTTCAGAC 
GTCAAAATGG GCAGTCCTGC CCATCGCAGT GGCTCCCTTC GATCAGGGGA CATTCTGCTT 
GCCGTGGACC AGCATCCAGT GCAGCATTTC AACGTGGACG CACTGCTCAA GGAGGAGGGC 
AGATCACAGA ATCCCAGCCA CAGCTCCTCG GACTTCACCA CGCTGACGAT CAAGCGGATC 
GTCCTGCCCG ACTTCCTGCC CATGTCCAGT CCGATTTACA GCAATTGCCC GGCGAGTGGC 
ATAAACTTGG GCATGGGCAT GGGTGTCTCC ACAAGCACGG ATCACGATCT TTACAGCAGT 
GCCTATGTGA CGGCGGGCAA GTATGCAGAT TGCGTGTCCC TGAAATCGAG GACTCCGCAA 
CCGGATTACT TCCGAGTGCC CAGCATGGAC GATGCCAGTC TGCAGTCCGT GCAGTTGCGG 
TCTGGATCGG GCTCGAATGG AGGTAACCAG GCGGCTAACA GCTGGTCCAC CGGGGTCAAC 
AGTCGATCCT ATGTGGCCCC ACCCAATACG CAGAGTCTAA CCACCGAGTT GCCCGAGGAG 
GAGGACGAGC AGGAGCAAAT GTACCCCGGA TACGAGCTCA ATCGCTATGC CAGTGTCGAC 
TGCACCGCCT TGCCGCCGCC CATGGAGAAC AAGGTGTACG GATCGGCGGC CAGTTCGAGC 
AGCAAGAGCA GCGGTAGCAG TCTGCACCAG ATCATCTTCA CGGTGCGCTT GGAGCCCAAA 
GGAGGATTGC TCGGCATCAC TTTGGCCGGC AGCGAGGATA TCACCAAGTC CATTACGATT 
AGTGGCCTTG TAGAGGGTGG CATTGCTCAC AAGAATGGCC AGATCCACGT GGGTGACCAG 
CTGCTGGCCA TCGATGAGCA CTCGGTGCAG GGCATGCCCC TTTCACATGC CACCAGTCTG 
CTGCAGAATC TCGGCGATCT GGTCGACCTG AAGATCCTGC GCAGCCACGA TTTGGCCAAT 
GGCAGTCATC TGCCGCAGAC GCAGGCCATT TATGCCAAGG TGCAACGGCG TCCCCGGAGT 
CCGTCGGCGA ATACGGAGGC CAGCAAGGAG TCGGGTAGCG GGAATGCCAA CGGTGGCGGA 
GCAGTCAATG GGAATTCGGG CAGTGGAAAG CCCCGGGTGT TTCATGTCAC CCTGTACAAG 
GACAAGGTGT ACGATGACTA TGGATTCTCG GTATCCGATG GACTGTACGA GCGTGGCGTC 
TTCATCAACA GGATTCGCAG CGGAGGTCCT GCCGATATGT GCGGCCTACT GAAGCCCTTC 
GATCGGATCA TGCAGGTAAA TGAAATGAAG ACGCAGGACT TCGATTGCTG TCTTACCGTT 
CCGCTGATCG CCGCCGCTGG CGATAAAATC GAGATGATCA TGCAGCGCTC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572285.2
CDS	288..3464
Translation	MKLWKSKKPIGGCVPGKSAALKQDQQQQQQQQQESHNNSFNGHHHPPTDTALAPMLSVDRAMSPAQSEDSGLAPERGTTYATITLPRNALHLAITFAERNDLSYPPVVGSLNPVGHAADFLAPGDRLHQIDGISTIGLSNQKVMNMLCAGGSDACPAIVEIEYSLPECTVSQNSLCVTSKLAQITVERESGCLGLTLRGGADYPLIVTHVRPHGPVYKTGRIKPGDRLLRVDNVSLIGKTLAEAQQIIKCGGHVSGYTNLTIEYDVSVVQSVEFSMGPLLIEIERPMNDKLGLVLCNYTAAVPASGSGSSTPSSGEKIEESAGVFIASILPASIADRCGALSVGDQVLSIDDTMIEHTAFSPDEVMTILDTSTGRGYTQMQIMPAHALARRGHTTLGSPKYSFSTLESRKSSTAGRQRQRFARKSSLPLENPACGTPGSTGGSMGTGGIHGSSSLGMGLGLCRAESFPVLLDCSHGAGIILSETVSGSGGSGAVAIAQIVPDSVADRSGCIQAGDRIVAINKMYSLDAAAMRQLLEGGSSRNGASNGTPANWLELEIEFDMPDAVVPASGVFSVKLLRAGKCGLGLSVSGSSHGGLVISDVKMGSPAHRSGSLRSGDILLAVDQHPVQHFNVDALLKEEGRSQNPSHSSSDFTTLTIKRIVLPDFLPMSSPIYSNCPASGINLGMGMGVSTSTDHDLYSSAYVTAGKYADCVSLKSRTPQPDYFRVPSMDDASLQSVQLRSGSGSNGGNQAANSWSTGVNSRSYVAPPNTQSLTTELPEEEDEQEQMYPGYELNRYASVDCTALPPPMENKVYGSAASSSSKSSGSSLHQIIFTVRLEPKGGLLGITLAGSEDITKSITISGLVEGGIAHKNGQIHVGDQLLAIDEHSVQGMPLSHATSLLQNLGDLVDLKILRSHDLANGSHLPQTQAIYAKVQRRPRSPSANTEASKESGSGNANGGGAVNGNSGSGKPRVFHVTLYKDKVYDDYGFSVSDGLYERGVFINRIRSGGPADMCGLLKPFDRIMQVNEMKTQDFDCCLTVPLIAAAGDKIEMIMQRSE

Target ORF information:

RefSeq Version	NM_132057.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamate receptor binding protein, transcript variant A (Grip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132057.3

ORF Insert Sequence:

ATGAAACTGT GGAAATCGAA GAAGCCCATT GGTGGCTGTG TGCCCGGAAA ATCGGCCGCC 
CTAAAACAGG ACCAACAGCA GCAACAACAA CAGCAACAGG AGTCGCACAA CAACTCCTTC 
AATGGACACC ATCATCCGCC GACGGACACT GCCTTGGCGC CCATGTTATC CGTTGACCGG 
GCGATGAGTC CGGCGCAATC GGAGGACTCC GGATTGGCAC CAGAAAGGGG TACCACCTAC 
GCCACCATCA CTCTGCCACG GAATGCCCTG CATTTGGCCA TCACATTCGC AGAACGCAAC 
GATCTGTCGT ATCCACCGGT GGTGGGCTCC CTAAATCCGG TGGGTCATGC GGCGGACTTC 
CTGGCGCCCG GTGATCGTCT CCACCAGATC GACGGCATAT CGACAATTGG TTTGAGCAAC 
CAGAAGGTGA TGAACATGCT CTGCGCTGGA GGATCGGATG CGTGTCCAGC CATCGTGGAG 
ATCGAGTACT CTCTGCCCGA ATGCACAGTT TCCCAGAACA GCCTTTGTGT GACCTCGAAG 
CTGGCGCAGA TCACCGTGGA GCGGGAGAGC GGATGCCTGG GCCTGACGTT GAGGGGCGGT 
GCCGACTACC CGCTGATCGT CACCCATGTG AGGCCCCATG GACCAGTGTA TAAGACCGGT 
CGCATCAAGC CAGGCGATCG GTTGCTGCGC GTCGATAATG TCTCACTGAT TGGCAAGACG 
CTGGCGGAGG CGCAGCAGAT CATCAAGTGC GGCGGTCATG TTTCCGGCTA CACCAACCTG 
ACCATCGAGT ACGATGTTTC TGTGGTGCAG AGCGTTGAGT TCTCGATGGG ACCGCTGCTC 
ATCGAGATCG AGCGACCGAT GAACGACAAG TTGGGCCTGG TGCTGTGCAA TTATACGGCG 
GCTGTGCCCG CTTCCGGTTC CGGTTCCAGT ACTCCTTCCA GCGGGGAGAA GATCGAGGAA 
TCGGCAGGCG TCTTTATCGC CAGTATCCTG CCCGCCAGCA TTGCAGATCG TTGCGGCGCC 
TTATCCGTTG GCGATCAGGT GCTCTCCATC GACGACACAA TGATCGAGCA TACCGCCTTC 
AGTCCCGACG AGGTGATGAC CATTTTGGAC ACCAGCACGG GTCGCGGCTA CACGCAGATG 
CAGATCATGC CCGCTCACGC GCTGGCACGT CGCGGTCATA CAACCTTGGG TAGTCCCAAG 
TACAGCTTTA GCACACTGGA GTCCCGTAAA TCCTCGACGG CGGGTCGCCA GCGTCAGCGT 
TTCGCGAGGA AAAGTTCCCT ACCGTTGGAG AATCCTGCAT GCGGCACTCC AGGCTCCACG 
GGAGGATCGA TGGGTACTGG TGGCATCCAT GGCAGCAGCA GCTTGGGCAT GGGTCTGGGC 
CTCTGCCGGG CGGAGAGCTT TCCCGTTCTC CTCGATTGCA GCCATGGAGC GGGCATTATC 
TTGTCTGAAA CGGTATCCGG ATCTGGAGGA TCGGGTGCTG TAGCCATTGC TCAGATCGTG 
CCGGATTCGG TGGCCGATCG CAGTGGCTGC ATTCAGGCGG GAGATCGCAT CGTGGCCATT 
AACAAGATGT ACAGCTTGGA TGCGGCGGCC ATGAGGCAAC TGCTCGAAGG AGGATCCAGT 
CGCAATGGAG CAAGCAATGG AACGCCGGCC AACTGGCTAG AACTGGAGAT CGAGTTCGAT 
ATGCCGGATG CCGTGGTGCC CGCCAGTGGT GTCTTTAGTG TCAAATTGCT CAGGGCCGGC 
AAATGTGGAC TGGGTCTGAG TGTGAGTGGT TCCAGTCATG GTGGTCTGGT CATTTCAGAC 
GTCAAAATGG GCAGTCCTGC CCATCGCAGT GGCTCCCTTC GATCAGGGGA CATTCTGCTT 
GCCGTGGACC AGCATCCAGT GCAGCATTTC AACGTGGACG CACTGCTCAA GGAGGAGGGC 
AGATCACAGA ATCCCAGCCA CAGCTCCTCG GACTTCACCA CGCTGACGAT CAAGCGGATC 
GTCCTGCCCG ACTTCCTGCC CATGTCCAGT CCGATTTACA GCAATTGCCC GGCGAGTGGC 
ATAAACTTGG GCATGGGCAT GGGTGTCTCC ACAAGCACGG ATCACGATCT TTACAGCAGT 
GCCTATGTGA CGGCGGGCAA GTATGCAGAT TGCGTGTCCC TGAAATCGAG GACTCCGCAA 
CCGGATTACT TCCGAGTGCC CAGCATGGAC GATGCCAGTC TGCAGTCCGT GCAGTTGCGG 
TCTGGATCGG GCTCGAATGG AGGTAACCAG GCGGCTAACA GCTGGTCCAC CGGGGTCAAC 
AGTCGATCCT ATGTGGCCCC ACCCAATACG CAGAGTCTAA CCACCGAGTT GCCCGAGGAG 
GAGGACGAGC AGGAGCAAAT GTACCCCGGA TACGAGCTCA ATCGCTATGC CAGTGTCGAC 
TGCACCGCCT TGCCGCCGCC CATGGAGAAC AAGGTGTACG GATCGGCGGC CAGTTCGAGC 
AGCAAGAGCA GCGGTAGCAG TCTGCACCAG ATCATCTTCA CGGTGCGCTT GGAGCCCAAA 
GGAGGATTGC TCGGCATCAC TTTGGCCGGC AGCGAGGATA TCACCAAGTC CATTACGATT 
AGTGGCCTTG TAGAGGGTGG CATTGCTCAC AAGAATGGCC AGATCCACGT GGGTGACCAG 
CTGCTGGCCA TCGATGAGCA CTCGGTGCAG GGCATGCCCC TTTCACATGC CACCAGTCTG 
CTGCAGAATC TCGGCGATCT GGTCGACCTG AAGATCCTGC GCAGCCACGA TTTGGCCAAT 
GGCAGTCATC TGCCGCAGAC GCAGGCCATT TATGCCAAGG TGCAACGGCG TCCCCGGAGT 
CCGTCGGCGA ATACGGAGGC CAGCAAGGAG TCGGGTAGCG GGAATGCCAA CGGTGGCGGA 
GCAGTCAATG GGAATTCGGG CAGTGGAAAG CCCCGGGTGT TTCATGTCAC CCTGTACAAG 
GACAAGGTGT ACGATGACTA TGGATTCTCG GTATCCGATG GACTGTACGA GCGTGGCGTC 
TTCATCAACA GGATTCGCAG CGGAGGTCCT GCCGATATGT GCGGCCTACT GAAGCCCTTC 
GATCGGATCA TGCAGGTAAA TGAAATGAAG ACGCAGGACT TCGATTGCTG TCTTACCGTT 
CCGCTGATCG CCGCCGCTGG CGATAAAATC GAGATGATCA TGCAGCGCTC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grip ( NM_001297992.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284921.1 Drosophila melanogaster glutamate receptor binding protein, transcript variant C (Grip), mRNA.

Price & Availability↑

CloneID	OFa22285
Clone ID Related Accession (Same CDS sequence)	NM_001297992.1 , NM_001297991.1
Accession Version	NM_001297992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glutamate receptor binding protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGT GGAAATCGAA GAAGCCCATT GGTGGCTGTG TGCCCGGAAA ATCGGCCGCC 
CTAAAACAGG ACCAACAGCA GCAACAACAA CAGCAACAGG AGTCGCACAA CAACTCCTTC 
AATGGACACC ATCATCCGCC GACGGACACT GCCTTGGCGC CCATGTTATC CGTTGACCGG 
GCGATGAGTC CGGCGCAATC GGAGGACTCC GGATTGGCAC CAGAAAGGGG TACCACCTAC 
GCCACCATCA CTCTGCCACG GAATGCCCTG CATTTGGCCA TCACATTCGC AGAACGCAAC 
GATCTGTCGT ATCCACCGGT GGTGGGCTCC CTAAATCCGG TGGGTCATGC GGCGGACTTC 
CTGGCGCCCG GTGATCGTCT CCACCAGATC GACGGCATAT CGACAATTGG TTTGAGCAAC 
CAGAAGGTGA TGAACATGCT CTGCGCTGGA GGATCGGATG CGTGTCCAGC CATCGTGGAG 
ATCGAGTACT CTCTGCCCGA ATGCATTTCC CAGAACAGCC TTTGTGTGAC CTCGAAGCTG 
GCGCAGATCA CCGTGGAGCG GGAGAGCGGA TGCCTGGGCC TGACGTTGAG GGGCGGTGCC 
GACTACCCGC TGATCGTCAC CCATGTGAGG CCCCATGGAC CAGTGTATAA GACCGGTCGC 
ATCAAGCCAG GCGATCGGTT GCTGCGCGTC GATAATGTCT CACTGATTGG CAAGACGCTG 
GCGGAGGCGC AGCAGATCAT CAAGTGCGGC GGTCATGTTT CCGGCTACAC CAACCTGACC 
ATCGAGTACG ATGTTTCTGT GGTGCAGAGC GTTGAGTTCT CGATGGGACC GCTGCTCATC 
GAGATCGAGC GACCGATGAA CGACAAGTTG GGCCTGGTGC TGTGCAATTA TACGGCGGCT 
GTGCCCGCTT CCGGTTCCGG TTCCAGTACT CCTTCCAGCG GGGAGAAGAT CGAGGAATCG 
GCAGGCGTCT TTATCGCCAG TATCCTGCCC GCCAGCATTG CAGATCGTTG CGGCGCCTTA 
TCCGTTGGCG ATCAGGTGCT CTCCATCGAC GACACAATGA TCGAGCATAC CGCCTTCAGT 
CCCGACGAGG TGATGACCAT TTTGGACACC AGCACGGGTC GCGGCTACAC GCAGATGCAG 
ATCATGCCCG CTCACGCGCT GGCACGTCGC GGTCATACAA CCTTGGGTAG TCCCAAGTAC 
AGCTTTAGCA CACTGGAGTC CCGTAAATCC TCGACGGCGG GTCGCCAGCG TCAGCGTTTC 
GCGAGGAAAA GTTCCCTACC GTTGGAGAAT CCTGCATGCG GCACTCCAGG CTCCACGGGA 
GGATCGATGG GTACTGGTGG CATCCATGGC AGCAGCAGCT TGGGCATGGG TCTGGGCCTC 
TGCCGGGCGG AGAGCTTTCC CGTTCTCCTC GATTGCAGCC ATGGAGCGGG CATTATCTTG 
TCTGAAACGG TATCCGGATC TGGAGGATCG GGTGCTGTAG CCATTGCTCA GATCGTGCCG 
GATTCGGTGG CCGATCGCAG TGGCTGCATT CAGGCGGGAG ATCGCATCGT GGCCATTAAC 
AAGATGTACA GCTTGGATGC GGCGGCCATG AGGCAACTGC TCGAAGGAGG ATCCAGTCGC 
AATGGAGCAA GCAATGGAAC GCCGGCCAAC TGGCTAGAAC TGGAGATCGA GTTCGATATG 
CCGGATGCCG TGGTGCCCGC CAGTGGTGTC TTTAGTGTCA AATTGCTCAG GGCCGGCAAA 
TGTGGACTGG GTCTGAGTGT GAGTGGTTCC AGTCATGGTG GTCTGGTCAT TTCAGACGTC 
AAAATGGGCA GTCCTGCCCA TCGCAGTGGC TCCCTTCGAT CAGGGGACAT TCTGCTTGCC 
GTGGACCAGC ATCCAGTGCA GCATTTCAAC GTGGACGCAC TGCTCAAGGA GGAGGGCAGA 
TCACAGAATC CCAGCCACAG CTCCTCGGAC TTCACCACGC TGACGATCAA GCGGATCGTC 
CTGCCCGACT TCCTGCCCAT GTCCAGTCCG ATTTACAGCA ATTGCCCGGC GAGTGGCATA 
AACTTGGGCA TGGGCATGGG TGTCTCCACA AGCACGGATC ACGATCTTTA CAGCAGTGCC 
TATGTGACGG CGGGCAAGTA TGCAGATTGC GTGTCCCTGA AATCGAGGAC TCCGCAACCG 
GATTACTTCC GAGTGCCCAG CATGGACGAT GCCAGTCTGC AGTCCGTGCA GTTGCGGTCT 
GGATCGGGCT CGAATGGAGG TAACCAGGCG GCTAACAGCT GGTCCACCGG GGTCAACAGT 
CGATCCTATG TGGCCCCACC CAATACGCAG AGTCTAACCA CCGAGTTGCC CGAGGAGGAG 
GACGAGCAGG AGCAAATGTA CCCCGGATAC GAGCTCAATC GCTATGCCAG TGTCGACTGC 
ACCGCCTTGC CGCCGCCCAT GGAGAACAAG GTGTACGGAT CGGCGGCCAG TTCGAGCAGC 
AAGAGCAGCG GTAGCAGTCT GCACCAGATC ATCTTCACGG TGCGCTTGGA GCCCAAAGGA 
GGATTGCTCG GCATCACTTT GGCCGGCAGC GAGGATATCA CCAAGTCCAT TACGATTAGT 
GGCCTTGTAG AGGGTGGCAT TGCTCACAAG AATGGCCAGA TCCACGTGGG TGACCAGCTG 
CTGGCCATCG ATGAGCACTC GGTGCAGGGC ATGCCCCTTT CACATGCCAC CAGTCTGCTG 
CAGAATCTCG GCGATCTGGT CGACCTGAAG ATCCTGCGCA GCCACGATTT GGCCAATGGC 
AGTCATCTGC CGCAGACGCA GGCCATTTAT GCCAAGGTGC AACGGCGTCC CCGGAGTCCG 
TCGGCGAATA CGGAGGCCAG CAAGGAGTCG GGTAGCGGGA ATGCCAACGG TGGCGGAGCA 
GTCAATGGGA ATTCGGGCAG TGGAAAGCCC CGGGTGTTTC ATGTCACCCT GTACAAGGAC 
AAGGTGTACG ATGACTATGG ATTCTCGGTA TCCGATGGAC TGTACGAGCG TGGCGTCTTC 
ATCAACAGGA TTCGCAGCGG AGGTCCTGCC GATATGTGCG GCCTACTGAA GCCCTTCGAT 
CGGATCATGC AGGTAAATGA AATGAAGACG CAGGACTTCG ATTGCTGTCT TACCGTTCCG 
CTGATCGCCG CCGCTGGCGA TAAAATCGAG ATGATCATGC AGCGCTCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284921.1
CDS	104..3277
Translation	MKLWKSKKPIGGCVPGKSAALKQDQQQQQQQQQESHNNSFNGHHHPPTDTALAPMLSVDRAMSPAQSEDSGLAPERGTTYATITLPRNALHLAITFAERNDLSYPPVVGSLNPVGHAADFLAPGDRLHQIDGISTIGLSNQKVMNMLCAGGSDACPAIVEIEYSLPECISQNSLCVTSKLAQITVERESGCLGLTLRGGADYPLIVTHVRPHGPVYKTGRIKPGDRLLRVDNVSLIGKTLAEAQQIIKCGGHVSGYTNLTIEYDVSVVQSVEFSMGPLLIEIERPMNDKLGLVLCNYTAAVPASGSGSSTPSSGEKIEESAGVFIASILPASIADRCGALSVGDQVLSIDDTMIEHTAFSPDEVMTILDTSTGRGYTQMQIMPAHALARRGHTTLGSPKYSFSTLESRKSSTAGRQRQRFARKSSLPLENPACGTPGSTGGSMGTGGIHGSSSLGMGLGLCRAESFPVLLDCSHGAGIILSETVSGSGGSGAVAIAQIVPDSVADRSGCIQAGDRIVAINKMYSLDAAAMRQLLEGGSSRNGASNGTPANWLELEIEFDMPDAVVPASGVFSVKLLRAGKCGLGLSVSGSSHGGLVISDVKMGSPAHRSGSLRSGDILLAVDQHPVQHFNVDALLKEEGRSQNPSHSSSDFTTLTIKRIVLPDFLPMSSPIYSNCPASGINLGMGMGVSTSTDHDLYSSAYVTAGKYADCVSLKSRTPQPDYFRVPSMDDASLQSVQLRSGSGSNGGNQAANSWSTGVNSRSYVAPPNTQSLTTELPEEEDEQEQMYPGYELNRYASVDCTALPPPMENKVYGSAASSSSKSSGSSLHQIIFTVRLEPKGGLLGITLAGSEDITKSITISGLVEGGIAHKNGQIHVGDQLLAIDEHSVQGMPLSHATSLLQNLGDLVDLKILRSHDLANGSHLPQTQAIYAKVQRRPRSPSANTEASKESGSGNANGGGAVNGNSGSGKPRVFHVTLYKDKVYDDYGFSVSDGLYERGVFINRIRSGGPADMCGLLKPFDRIMQVNEMKTQDFDCCLTVPLIAAAGDKIEMIMQRSE

Target ORF information:

RefSeq Version	NM_001297992.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamate receptor binding protein, transcript variant C (Grip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297992.1

ORF Insert Sequence:

ATGAAACTGT GGAAATCGAA GAAGCCCATT GGTGGCTGTG TGCCCGGAAA ATCGGCCGCC 
CTAAAACAGG ACCAACAGCA GCAACAACAA CAGCAACAGG AGTCGCACAA CAACTCCTTC 
AATGGACACC ATCATCCGCC GACGGACACT GCCTTGGCGC CCATGTTATC CGTTGACCGG 
GCGATGAGTC CGGCGCAATC GGAGGACTCC GGATTGGCAC CAGAAAGGGG TACCACCTAC 
GCCACCATCA CTCTGCCACG GAATGCCCTG CATTTGGCCA TCACATTCGC AGAACGCAAC 
GATCTGTCGT ATCCACCGGT GGTGGGCTCC CTAAATCCGG TGGGTCATGC GGCGGACTTC 
CTGGCGCCCG GTGATCGTCT CCACCAGATC GACGGCATAT CGACAATTGG TTTGAGCAAC 
CAGAAGGTGA TGAACATGCT CTGCGCTGGA GGATCGGATG CGTGTCCAGC CATCGTGGAG 
ATCGAGTACT CTCTGCCCGA ATGCATTTCC CAGAACAGCC TTTGTGTGAC CTCGAAGCTG 
GCGCAGATCA CCGTGGAGCG GGAGAGCGGA TGCCTGGGCC TGACGTTGAG GGGCGGTGCC 
GACTACCCGC TGATCGTCAC CCATGTGAGG CCCCATGGAC CAGTGTATAA GACCGGTCGC 
ATCAAGCCAG GCGATCGGTT GCTGCGCGTC GATAATGTCT CACTGATTGG CAAGACGCTG 
GCGGAGGCGC AGCAGATCAT CAAGTGCGGC GGTCATGTTT CCGGCTACAC CAACCTGACC 
ATCGAGTACG ATGTTTCTGT GGTGCAGAGC GTTGAGTTCT CGATGGGACC GCTGCTCATC 
GAGATCGAGC GACCGATGAA CGACAAGTTG GGCCTGGTGC TGTGCAATTA TACGGCGGCT 
GTGCCCGCTT CCGGTTCCGG TTCCAGTACT CCTTCCAGCG GGGAGAAGAT CGAGGAATCG 
GCAGGCGTCT TTATCGCCAG TATCCTGCCC GCCAGCATTG CAGATCGTTG CGGCGCCTTA 
TCCGTTGGCG ATCAGGTGCT CTCCATCGAC GACACAATGA TCGAGCATAC CGCCTTCAGT 
CCCGACGAGG TGATGACCAT TTTGGACACC AGCACGGGTC GCGGCTACAC GCAGATGCAG 
ATCATGCCCG CTCACGCGCT GGCACGTCGC GGTCATACAA CCTTGGGTAG TCCCAAGTAC 
AGCTTTAGCA CACTGGAGTC CCGTAAATCC TCGACGGCGG GTCGCCAGCG TCAGCGTTTC 
GCGAGGAAAA GTTCCCTACC GTTGGAGAAT CCTGCATGCG GCACTCCAGG CTCCACGGGA 
GGATCGATGG GTACTGGTGG CATCCATGGC AGCAGCAGCT TGGGCATGGG TCTGGGCCTC 
TGCCGGGCGG AGAGCTTTCC CGTTCTCCTC GATTGCAGCC ATGGAGCGGG CATTATCTTG 
TCTGAAACGG TATCCGGATC TGGAGGATCG GGTGCTGTAG CCATTGCTCA GATCGTGCCG 
GATTCGGTGG CCGATCGCAG TGGCTGCATT CAGGCGGGAG ATCGCATCGT GGCCATTAAC 
AAGATGTACA GCTTGGATGC GGCGGCCATG AGGCAACTGC TCGAAGGAGG ATCCAGTCGC 
AATGGAGCAA GCAATGGAAC GCCGGCCAAC TGGCTAGAAC TGGAGATCGA GTTCGATATG 
CCGGATGCCG TGGTGCCCGC CAGTGGTGTC TTTAGTGTCA AATTGCTCAG GGCCGGCAAA 
TGTGGACTGG GTCTGAGTGT GAGTGGTTCC AGTCATGGTG GTCTGGTCAT TTCAGACGTC 
AAAATGGGCA GTCCTGCCCA TCGCAGTGGC TCCCTTCGAT CAGGGGACAT TCTGCTTGCC 
GTGGACCAGC ATCCAGTGCA GCATTTCAAC GTGGACGCAC TGCTCAAGGA GGAGGGCAGA 
TCACAGAATC CCAGCCACAG CTCCTCGGAC TTCACCACGC TGACGATCAA GCGGATCGTC 
CTGCCCGACT TCCTGCCCAT GTCCAGTCCG ATTTACAGCA ATTGCCCGGC GAGTGGCATA 
AACTTGGGCA TGGGCATGGG TGTCTCCACA AGCACGGATC ACGATCTTTA CAGCAGTGCC 
TATGTGACGG CGGGCAAGTA TGCAGATTGC GTGTCCCTGA AATCGAGGAC TCCGCAACCG 
GATTACTTCC GAGTGCCCAG CATGGACGAT GCCAGTCTGC AGTCCGTGCA GTTGCGGTCT 
GGATCGGGCT CGAATGGAGG TAACCAGGCG GCTAACAGCT GGTCCACCGG GGTCAACAGT 
CGATCCTATG TGGCCCCACC CAATACGCAG AGTCTAACCA CCGAGTTGCC CGAGGAGGAG 
GACGAGCAGG AGCAAATGTA CCCCGGATAC GAGCTCAATC GCTATGCCAG TGTCGACTGC 
ACCGCCTTGC CGCCGCCCAT GGAGAACAAG GTGTACGGAT CGGCGGCCAG TTCGAGCAGC 
AAGAGCAGCG GTAGCAGTCT GCACCAGATC ATCTTCACGG TGCGCTTGGA GCCCAAAGGA 
GGATTGCTCG GCATCACTTT GGCCGGCAGC GAGGATATCA CCAAGTCCAT TACGATTAGT 
GGCCTTGTAG AGGGTGGCAT TGCTCACAAG AATGGCCAGA TCCACGTGGG TGACCAGCTG 
CTGGCCATCG ATGAGCACTC GGTGCAGGGC ATGCCCCTTT CACATGCCAC CAGTCTGCTG 
CAGAATCTCG GCGATCTGGT CGACCTGAAG ATCCTGCGCA GCCACGATTT GGCCAATGGC 
AGTCATCTGC CGCAGACGCA GGCCATTTAT GCCAAGGTGC AACGGCGTCC CCGGAGTCCG 
TCGGCGAATA CGGAGGCCAG CAAGGAGTCG GGTAGCGGGA ATGCCAACGG TGGCGGAGCA 
GTCAATGGGA ATTCGGGCAG TGGAAAGCCC CGGGTGTTTC ATGTCACCCT GTACAAGGAC 
AAGGTGTACG ATGACTATGG ATTCTCGGTA TCCGATGGAC TGTACGAGCG TGGCGTCTTC 
ATCAACAGGA TTCGCAGCGG AGGTCCTGCC GATATGTGCG GCCTACTGAA GCCCTTCGAT 
CGGATCATGC AGGTAAATGA AATGAAGACG CAGGACTTCG ATTGCTGTCT TACCGTTCCG 
CTGATCGCCG CCGCTGGCGA TAAAATCGAG ATGATCATGC AGCGCTCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grip ( NM_001297991.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284920.1 Drosophila melanogaster glutamate receptor binding protein, transcript variant B (Grip), mRNA.

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CloneID	OFa22285
Clone ID Related Accession (Same CDS sequence)	NM_001297992.1 , NM_001297991.1
Accession Version	NM_001297991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glutamate receptor binding protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACTGT GGAAATCGAA GAAGCCCATT GGTGGCTGTG TGCCCGGAAA ATCGGCCGCC 
CTAAAACAGG ACCAACAGCA GCAACAACAA CAGCAACAGG AGTCGCACAA CAACTCCTTC 
AATGGACACC ATCATCCGCC GACGGACACT GCCTTGGCGC CCATGTTATC CGTTGACCGG 
GCGATGAGTC CGGCGCAATC GGAGGACTCC GGATTGGCAC CAGAAAGGGG TACCACCTAC 
GCCACCATCA CTCTGCCACG GAATGCCCTG CATTTGGCCA TCACATTCGC AGAACGCAAC 
GATCTGTCGT ATCCACCGGT GGTGGGCTCC CTAAATCCGG TGGGTCATGC GGCGGACTTC 
CTGGCGCCCG GTGATCGTCT CCACCAGATC GACGGCATAT CGACAATTGG TTTGAGCAAC 
CAGAAGGTGA TGAACATGCT CTGCGCTGGA GGATCGGATG CGTGTCCAGC CATCGTGGAG 
ATCGAGTACT CTCTGCCCGA ATGCATTTCC CAGAACAGCC TTTGTGTGAC CTCGAAGCTG 
GCGCAGATCA CCGTGGAGCG GGAGAGCGGA TGCCTGGGCC TGACGTTGAG GGGCGGTGCC 
GACTACCCGC TGATCGTCAC CCATGTGAGG CCCCATGGAC CAGTGTATAA GACCGGTCGC 
ATCAAGCCAG GCGATCGGTT GCTGCGCGTC GATAATGTCT CACTGATTGG CAAGACGCTG 
GCGGAGGCGC AGCAGATCAT CAAGTGCGGC GGTCATGTTT CCGGCTACAC CAACCTGACC 
ATCGAGTACG ATGTTTCTGT GGTGCAGAGC GTTGAGTTCT CGATGGGACC GCTGCTCATC 
GAGATCGAGC GACCGATGAA CGACAAGTTG GGCCTGGTGC TGTGCAATTA TACGGCGGCT 
GTGCCCGCTT CCGGTTCCGG TTCCAGTACT CCTTCCAGCG GGGAGAAGAT CGAGGAATCG 
GCAGGCGTCT TTATCGCCAG TATCCTGCCC GCCAGCATTG CAGATCGTTG CGGCGCCTTA 
TCCGTTGGCG ATCAGGTGCT CTCCATCGAC GACACAATGA TCGAGCATAC CGCCTTCAGT 
CCCGACGAGG TGATGACCAT TTTGGACACC AGCACGGGTC GCGGCTACAC GCAGATGCAG 
ATCATGCCCG CTCACGCGCT GGCACGTCGC GGTCATACAA CCTTGGGTAG TCCCAAGTAC 
AGCTTTAGCA CACTGGAGTC CCGTAAATCC TCGACGGCGG GTCGCCAGCG TCAGCGTTTC 
GCGAGGAAAA GTTCCCTACC GTTGGAGAAT CCTGCATGCG GCACTCCAGG CTCCACGGGA 
GGATCGATGG GTACTGGTGG CATCCATGGC AGCAGCAGCT TGGGCATGGG TCTGGGCCTC 
TGCCGGGCGG AGAGCTTTCC CGTTCTCCTC GATTGCAGCC ATGGAGCGGG CATTATCTTG 
TCTGAAACGG TATCCGGATC TGGAGGATCG GGTGCTGTAG CCATTGCTCA GATCGTGCCG 
GATTCGGTGG CCGATCGCAG TGGCTGCATT CAGGCGGGAG ATCGCATCGT GGCCATTAAC 
AAGATGTACA GCTTGGATGC GGCGGCCATG AGGCAACTGC TCGAAGGAGG ATCCAGTCGC 
AATGGAGCAA GCAATGGAAC GCCGGCCAAC TGGCTAGAAC TGGAGATCGA GTTCGATATG 
CCGGATGCCG TGGTGCCCGC CAGTGGTGTC TTTAGTGTCA AATTGCTCAG GGCCGGCAAA 
TGTGGACTGG GTCTGAGTGT GAGTGGTTCC AGTCATGGTG GTCTGGTCAT TTCAGACGTC 
AAAATGGGCA GTCCTGCCCA TCGCAGTGGC TCCCTTCGAT CAGGGGACAT TCTGCTTGCC 
GTGGACCAGC ATCCAGTGCA GCATTTCAAC GTGGACGCAC TGCTCAAGGA GGAGGGCAGA 
TCACAGAATC CCAGCCACAG CTCCTCGGAC TTCACCACGC TGACGATCAA GCGGATCGTC 
CTGCCCGACT TCCTGCCCAT GTCCAGTCCG ATTTACAGCA ATTGCCCGGC GAGTGGCATA 
AACTTGGGCA TGGGCATGGG TGTCTCCACA AGCACGGATC ACGATCTTTA CAGCAGTGCC 
TATGTGACGG CGGGCAAGTA TGCAGATTGC GTGTCCCTGA AATCGAGGAC TCCGCAACCG 
GATTACTTCC GAGTGCCCAG CATGGACGAT GCCAGTCTGC AGTCCGTGCA GTTGCGGTCT 
GGATCGGGCT CGAATGGAGG TAACCAGGCG GCTAACAGCT GGTCCACCGG GGTCAACAGT 
CGATCCTATG TGGCCCCACC CAATACGCAG AGTCTAACCA CCGAGTTGCC CGAGGAGGAG 
GACGAGCAGG AGCAAATGTA CCCCGGATAC GAGCTCAATC GCTATGCCAG TGTCGACTGC 
ACCGCCTTGC CGCCGCCCAT GGAGAACAAG GTGTACGGAT CGGCGGCCAG TTCGAGCAGC 
AAGAGCAGCG GTAGCAGTCT GCACCAGATC ATCTTCACGG TGCGCTTGGA GCCCAAAGGA 
GGATTGCTCG GCATCACTTT GGCCGGCAGC GAGGATATCA CCAAGTCCAT TACGATTAGT 
GGCCTTGTAG AGGGTGGCAT TGCTCACAAG AATGGCCAGA TCCACGTGGG TGACCAGCTG 
CTGGCCATCG ATGAGCACTC GGTGCAGGGC ATGCCCCTTT CACATGCCAC CAGTCTGCTG 
CAGAATCTCG GCGATCTGGT CGACCTGAAG ATCCTGCGCA GCCACGATTT GGCCAATGGC 
AGTCATCTGC CGCAGACGCA GGCCATTTAT GCCAAGGTGC AACGGCGTCC CCGGAGTCCG 
TCGGCGAATA CGGAGGCCAG CAAGGAGTCG GGTAGCGGGA ATGCCAACGG TGGCGGAGCA 
GTCAATGGGA ATTCGGGCAG TGGAAAGCCC CGGGTGTTTC ATGTCACCCT GTACAAGGAC 
AAGGTGTACG ATGACTATGG ATTCTCGGTA TCCGATGGAC TGTACGAGCG TGGCGTCTTC 
ATCAACAGGA TTCGCAGCGG AGGTCCTGCC GATATGTGCG GCCTACTGAA GCCCTTCGAT 
CGGATCATGC AGGTAAATGA AATGAAGACG CAGGACTTCG ATTGCTGTCT TACCGTTCCG 
CTGATCGCCG CCGCTGGCGA TAAAATCGAG ATGATCATGC AGCGCTCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284920.1
CDS	153..3326
Translation	MKLWKSKKPIGGCVPGKSAALKQDQQQQQQQQQESHNNSFNGHHHPPTDTALAPMLSVDRAMSPAQSEDSGLAPERGTTYATITLPRNALHLAITFAERNDLSYPPVVGSLNPVGHAADFLAPGDRLHQIDGISTIGLSNQKVMNMLCAGGSDACPAIVEIEYSLPECISQNSLCVTSKLAQITVERESGCLGLTLRGGADYPLIVTHVRPHGPVYKTGRIKPGDRLLRVDNVSLIGKTLAEAQQIIKCGGHVSGYTNLTIEYDVSVVQSVEFSMGPLLIEIERPMNDKLGLVLCNYTAAVPASGSGSSTPSSGEKIEESAGVFIASILPASIADRCGALSVGDQVLSIDDTMIEHTAFSPDEVMTILDTSTGRGYTQMQIMPAHALARRGHTTLGSPKYSFSTLESRKSSTAGRQRQRFARKSSLPLENPACGTPGSTGGSMGTGGIHGSSSLGMGLGLCRAESFPVLLDCSHGAGIILSETVSGSGGSGAVAIAQIVPDSVADRSGCIQAGDRIVAINKMYSLDAAAMRQLLEGGSSRNGASNGTPANWLELEIEFDMPDAVVPASGVFSVKLLRAGKCGLGLSVSGSSHGGLVISDVKMGSPAHRSGSLRSGDILLAVDQHPVQHFNVDALLKEEGRSQNPSHSSSDFTTLTIKRIVLPDFLPMSSPIYSNCPASGINLGMGMGVSTSTDHDLYSSAYVTAGKYADCVSLKSRTPQPDYFRVPSMDDASLQSVQLRSGSGSNGGNQAANSWSTGVNSRSYVAPPNTQSLTTELPEEEDEQEQMYPGYELNRYASVDCTALPPPMENKVYGSAASSSSKSSGSSLHQIIFTVRLEPKGGLLGITLAGSEDITKSITISGLVEGGIAHKNGQIHVGDQLLAIDEHSVQGMPLSHATSLLQNLGDLVDLKILRSHDLANGSHLPQTQAIYAKVQRRPRSPSANTEASKESGSGNANGGGAVNGNSGSGKPRVFHVTLYKDKVYDDYGFSVSDGLYERGVFINRIRSGGPADMCGLLKPFDRIMQVNEMKTQDFDCCLTVPLIAAAGDKIEMIMQRSE

Target ORF information:

RefSeq Version	NM_001297991.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamate receptor binding protein, transcript variant B (Grip), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297991.1

ORF Insert Sequence:

ATGAAACTGT GGAAATCGAA GAAGCCCATT GGTGGCTGTG TGCCCGGAAA ATCGGCCGCC 
CTAAAACAGG ACCAACAGCA GCAACAACAA CAGCAACAGG AGTCGCACAA CAACTCCTTC 
AATGGACACC ATCATCCGCC GACGGACACT GCCTTGGCGC CCATGTTATC CGTTGACCGG 
GCGATGAGTC CGGCGCAATC GGAGGACTCC GGATTGGCAC CAGAAAGGGG TACCACCTAC 
GCCACCATCA CTCTGCCACG GAATGCCCTG CATTTGGCCA TCACATTCGC AGAACGCAAC 
GATCTGTCGT ATCCACCGGT GGTGGGCTCC CTAAATCCGG TGGGTCATGC GGCGGACTTC 
CTGGCGCCCG GTGATCGTCT CCACCAGATC GACGGCATAT CGACAATTGG TTTGAGCAAC 
CAGAAGGTGA TGAACATGCT CTGCGCTGGA GGATCGGATG CGTGTCCAGC CATCGTGGAG 
ATCGAGTACT CTCTGCCCGA ATGCATTTCC CAGAACAGCC TTTGTGTGAC CTCGAAGCTG 
GCGCAGATCA CCGTGGAGCG GGAGAGCGGA TGCCTGGGCC TGACGTTGAG GGGCGGTGCC 
GACTACCCGC TGATCGTCAC CCATGTGAGG CCCCATGGAC CAGTGTATAA GACCGGTCGC 
ATCAAGCCAG GCGATCGGTT GCTGCGCGTC GATAATGTCT CACTGATTGG CAAGACGCTG 
GCGGAGGCGC AGCAGATCAT CAAGTGCGGC GGTCATGTTT CCGGCTACAC CAACCTGACC 
ATCGAGTACG ATGTTTCTGT GGTGCAGAGC GTTGAGTTCT CGATGGGACC GCTGCTCATC 
GAGATCGAGC GACCGATGAA CGACAAGTTG GGCCTGGTGC TGTGCAATTA TACGGCGGCT 
GTGCCCGCTT CCGGTTCCGG TTCCAGTACT CCTTCCAGCG GGGAGAAGAT CGAGGAATCG 
GCAGGCGTCT TTATCGCCAG TATCCTGCCC GCCAGCATTG CAGATCGTTG CGGCGCCTTA 
TCCGTTGGCG ATCAGGTGCT CTCCATCGAC GACACAATGA TCGAGCATAC CGCCTTCAGT 
CCCGACGAGG TGATGACCAT TTTGGACACC AGCACGGGTC GCGGCTACAC GCAGATGCAG 
ATCATGCCCG CTCACGCGCT GGCACGTCGC GGTCATACAA CCTTGGGTAG TCCCAAGTAC 
AGCTTTAGCA CACTGGAGTC CCGTAAATCC TCGACGGCGG GTCGCCAGCG TCAGCGTTTC 
GCGAGGAAAA GTTCCCTACC GTTGGAGAAT CCTGCATGCG GCACTCCAGG CTCCACGGGA 
GGATCGATGG GTACTGGTGG CATCCATGGC AGCAGCAGCT TGGGCATGGG TCTGGGCCTC 
TGCCGGGCGG AGAGCTTTCC CGTTCTCCTC GATTGCAGCC ATGGAGCGGG CATTATCTTG 
TCTGAAACGG TATCCGGATC TGGAGGATCG GGTGCTGTAG CCATTGCTCA GATCGTGCCG 
GATTCGGTGG CCGATCGCAG TGGCTGCATT CAGGCGGGAG ATCGCATCGT GGCCATTAAC 
AAGATGTACA GCTTGGATGC GGCGGCCATG AGGCAACTGC TCGAAGGAGG ATCCAGTCGC 
AATGGAGCAA GCAATGGAAC GCCGGCCAAC TGGCTAGAAC TGGAGATCGA GTTCGATATG 
CCGGATGCCG TGGTGCCCGC CAGTGGTGTC TTTAGTGTCA AATTGCTCAG GGCCGGCAAA 
TGTGGACTGG GTCTGAGTGT GAGTGGTTCC AGTCATGGTG GTCTGGTCAT TTCAGACGTC 
AAAATGGGCA GTCCTGCCCA TCGCAGTGGC TCCCTTCGAT CAGGGGACAT TCTGCTTGCC 
GTGGACCAGC ATCCAGTGCA GCATTTCAAC GTGGACGCAC TGCTCAAGGA GGAGGGCAGA 
TCACAGAATC CCAGCCACAG CTCCTCGGAC TTCACCACGC TGACGATCAA GCGGATCGTC 
CTGCCCGACT TCCTGCCCAT GTCCAGTCCG ATTTACAGCA ATTGCCCGGC GAGTGGCATA 
AACTTGGGCA TGGGCATGGG TGTCTCCACA AGCACGGATC ACGATCTTTA CAGCAGTGCC 
TATGTGACGG CGGGCAAGTA TGCAGATTGC GTGTCCCTGA AATCGAGGAC TCCGCAACCG 
GATTACTTCC GAGTGCCCAG CATGGACGAT GCCAGTCTGC AGTCCGTGCA GTTGCGGTCT 
GGATCGGGCT CGAATGGAGG TAACCAGGCG GCTAACAGCT GGTCCACCGG GGTCAACAGT 
CGATCCTATG TGGCCCCACC CAATACGCAG AGTCTAACCA CCGAGTTGCC CGAGGAGGAG 
GACGAGCAGG AGCAAATGTA CCCCGGATAC GAGCTCAATC GCTATGCCAG TGTCGACTGC 
ACCGCCTTGC CGCCGCCCAT GGAGAACAAG GTGTACGGAT CGGCGGCCAG TTCGAGCAGC 
AAGAGCAGCG GTAGCAGTCT GCACCAGATC ATCTTCACGG TGCGCTTGGA GCCCAAAGGA 
GGATTGCTCG GCATCACTTT GGCCGGCAGC GAGGATATCA CCAAGTCCAT TACGATTAGT 
GGCCTTGTAG AGGGTGGCAT TGCTCACAAG AATGGCCAGA TCCACGTGGG TGACCAGCTG 
CTGGCCATCG ATGAGCACTC GGTGCAGGGC ATGCCCCTTT CACATGCCAC CAGTCTGCTG 
CAGAATCTCG GCGATCTGGT CGACCTGAAG ATCCTGCGCA GCCACGATTT GGCCAATGGC 
AGTCATCTGC CGCAGACGCA GGCCATTTAT GCCAAGGTGC AACGGCGTCC CCGGAGTCCG 
TCGGCGAATA CGGAGGCCAG CAAGGAGTCG GGTAGCGGGA ATGCCAACGG TGGCGGAGCA 
GTCAATGGGA ATTCGGGCAG TGGAAAGCCC CGGGTGTTTC ATGTCACCCT GTACAAGGAC 
AAGGTGTACG ATGACTATGG ATTCTCGGTA TCCGATGGAC TGTACGAGCG TGGCGTCTTC 
ATCAACAGGA TTCGCAGCGG AGGTCCTGCC GATATGTGCG GCCTACTGAA GCCCTTCGAT 
CGGATCATGC AGGTAAATGA AATGAAGACG CAGGACTTCG ATTGCTGTCT TACCGTTCCG 
CTGATCGCCG CCGCTGGCGA TAAAATCGAG ATGATCATGC AGCGCTCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Grip cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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