knk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	knk
Entrez Gene ID	47137
Full Name	knickkopf
Synonyms	CG6217,Dmel\CG6217,Gnarled,Knk,dyspneic,gnar,l(3)85Eg
General protein information	Preferred Names knickkopf Names CG6217 gene product from transcript CG6217-RA CG6217-PA dyspneic gnarled knk-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85F13-85F13\|3-49.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_141724.4	NP_649981.1	knickkopf

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	knk	NP_649981.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004500	XP_313797.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression 30 h prior and 48 h after pupariation is essential for correct wing cuticle formation
Title: Timed Knickkopf function is essential for wing cuticle formation in Drosophila melanogaster.

Results confirm the absolute necessity of tryptophan(299), methionine(333), and histidine(437) for Knickkopf function and stability, the latter two being predicted to be critical for heme binding.
Title: The Knickkopf DOMON domain is essential for cuticle differentiation in Drosophila melanogaster.

Knk and the predicted chitin-binding protein Rtv form membrane complexes essential for epithelial tubulogenesis and cuticle formation through their specific role in directing chitin filament assembly.
Title: Drosophila Knickkopf and Retroactive are needed for epithelial tube growth and cuticle differentiation through their specific requirement for chitin filament organization.

The following knk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the knk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06916	NM_141724.4 Latest version!	Drosophila melanogaster knickkopf (knk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

knk ( NM_141724.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649981.1 Drosophila melanogaster knickkopf (knk), mRNA.

Price & Availability↑

CloneID	OFa06916
Clone ID Related Accession (Same CDS sequence)	NM_141724.4
Accession Version	NM_141724.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	knickkopf
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141724.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTGA GCAGCAGAGT TCTTTGTCTG CGATTGCAGC ACCATTTATT ACTGGTTATT 
TTAACGATTT TCCTGCTTTG CCCCGCCGCC TCGGCCCAGA ACGAAGAAGA AGATGGTCCG 
TACCGGGGAA AATATCTGGG GAAACTGAAC TCCTACCATC ATCAAGTGTC CGGCGATGTG 
TACGCTGTGA ATGAGTACAC CTTTCTGATT GTGGGCTTTA ACTACGATGG CAACGGGGCG 
GATACGTTTT TCTGGTCGGG CGCTAGTAAT CGGCCGGGAC CGCAGGGATT CATTGTGCCC 
GATGAGTATG GCAAAACCAA CATTCTGGAT CGCTATCACA ACAAGGATTT CACGCTGACG 
CTGCCGGATC GAAAGAAGAT AACTGAGATT AAATGGCTGG CGGTTTACGA TCTGAGCAGT 
CAAAACAACT TTGGCGACGT TTATATACCC GAGGAGTTCG ATCCACCGAT GAGCCAATTG 
GGCGGAACCT TCTCCAAGCG CAGTCACAAT GTGAGCAGCA GCAGTGTTGA GATCTTGGAC 
TCAAAGACCA TTCGCATCAA GGACTTTACC TACGATGGGC GTGGCAAGAG GACATTTTTC 
TGGACAGGAG TCGGGCCACA GCCATCGAGT CGAGGAAGCA AGCTGCCAGA TGAGCGAGGA 
TACCTTGATC CTATAAGACA GTACAACAAG GAGACCATAG AACTGGAACT GCCAGGAGAT 
AAGACTATCT TTGACATCGA TTGGATCAGT GTATACGATG TGGCTGACAA CGAGAACTAT 
GGTCATGTTC TGTTCAACGA TAAACTTAAT GTTCCACCTT CCCTAGTGAA AGTTACACCA 
TTCGAATTCT CGCTGCCCAA TTGCCGGCAA CTACACAAGG ATATGCAGGT GTCCTGGGAG 
GTTTTCGGGC CGCAGATCAC GTTTCAACTA TCCGGTCAGG TGGGCGGCAA CGATTATATG 
TCTTTCGGCA TATCCGGATC TGATGTATCC AGCCAGATGA TTGGCTCCGA TGTCGTGGTG 
GCCTATATAG ACGACATCAG AGGCTACACC GTGGACTACA ATATCACTTC GTTAGCGCCG 
TGCGTTCAGG TTTTGGGTCA AAACAAAGGA GTTTGCCGTG ACGATGTGGT TGGTGGATTG 
GACAGCTTCC AGTTGAATAC GTATAGTCGC AAGGATGGCA TTAATACGAT CAGTTTTAGG 
CGGACGCTCA AGTCATCCGA TGATGGCGAT AAGGAGATCT TCCTAGACCG CAGCAACTAC 
GTGATCTGGG CATTTGGCCC ATTGGACTCC AACAACGAGC CCGCCTTTCA CACTTACTAT 
CCCAAGAGCG ATATAGTGAT AGATTTTAAC ACCACAGAAC CGGTTAATGA TTGCTTTGCA 
TTTACAAAAC GAGCGGAGAC GACTAATCCT CCAGTGTGGG AGCGAACCAG GATAACAGAT 
GCAACGGTAC GCACATTCAA CGCCTACTTG GGACCCTCTG GAGGACTTCG TGGCTATCAG 
GGATTGACGA ATCATGTGTC CAGTGGCTTG GCCTGGTACA TCAACGGCTA CATGATTCCA 
GAGCTTTACC TAAAGCGTGG TCTGACCTAC ACCTTTAAGG TTAGGGGTGG CAATAATCCT 
CACTCGCCGG AGCACTATCA TCCGTTGGTT ATCACCGATG ATCCCCAGGG TGGGTATGAC 
CGACTGTCTG ATGCAAAGCA GAGTGAGATT CGTGTATTAG CCGGTGTTGA ATTCACCCGG 
AGAGGCAGGC CCAAGCCCAC GGCTGCTGGT CCCCTGTGCC TGTCCAGATA TCCTCAAAAT 
AGTGATCGTC GACTGGATGA TAACTTCCCC ACTTTCAAGA AGTTCAATCG ATCCCTCATC 
ACCGAGTGTG TAGAAGGGGA ACCGGCTCTA TTGGAAATTA CACCGAATAT CACATGGCCG 
GATACTGTGT ACTACAACTC CTTTACCCAT GGCAATATGG GCTGGAAGAT TCACATCGTG 
GACAGTTATA CCAACTTGAA GAGTGGATCG ATGGGCTTAT CCTGGTCCTT GTGCATCATC 
CTGCTACCGT GGCTCGTGCT CCAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649981.1
CDS	446..2515
Translation	MALSSRVLCLRLQHHLLLVILTIFLLCPAASAQNEEEDGPYRGKYLGKLNSYHHQVSGDVYAVNEYTFLIVGFNYDGNGADTFFWSGASNRPGPQGFIVPDEYGKTNILDRYHNKDFTLTLPDRKKITEIKWLAVYDLSSQNNFGDVYIPEEFDPPMSQLGGTFSKRSHNVSSSSVEILDSKTIRIKDFTYDGRGKRTFFWTGVGPQPSSRGSKLPDERGYLDPIRQYNKETIELELPGDKTIFDIDWISVYDVADNENYGHVLFNDKLNVPPSLVKVTPFEFSLPNCRQLHKDMQVSWEVFGPQITFQLSGQVGGNDYMSFGISGSDVSSQMIGSDVVVAYIDDIRGYTVDYNITSLAPCVQVLGQNKGVCRDDVVGGLDSFQLNTYSRKDGINTISFRRTLKSSDDGDKEIFLDRSNYVIWAFGPLDSNNEPAFHTYYPKSDIVIDFNTTEPVNDCFAFTKRAETTNPPVWERTRITDATVRTFNAYLGPSGGLRGYQGLTNHVSSGLAWYINGYMIPELYLKRGLTYTFKVRGGNNPHSPEHYHPLVITDDPQGGYDRLSDAKQSEIRVLAGVEFTRRGRPKPTAAGPLCLSRYPQNSDRRLDDNFPTFKKFNRSLITECVEGEPALLEITPNITWPDTVYYNSFTHGNMGWKIHIVDSYTNLKSGSMGLSWSLCIILLPWLVLQN

Target ORF information:

RefSeq Version	NM_141724.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster knickkopf (knk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141724.4

ORF Insert Sequence:

ATGGCATTGA GCAGCAGAGT TCTTTGTCTG CGATTGCAGC ACCATTTATT ACTGGTTATT 
TTAACGATTT TCCTGCTTTG CCCCGCCGCC TCGGCCCAGA ACGAAGAAGA AGATGGTCCG 
TACCGGGGAA AATATCTGGG GAAACTGAAC TCCTACCATC ATCAAGTGTC CGGCGATGTG 
TACGCTGTGA ATGAGTACAC CTTTCTGATT GTGGGCTTTA ACTACGATGG CAACGGGGCG 
GATACGTTTT TCTGGTCGGG CGCTAGTAAT CGGCCGGGAC CGCAGGGATT CATTGTGCCC 
GATGAGTATG GCAAAACCAA CATTCTGGAT CGCTATCACA ACAAGGATTT CACGCTGACG 
CTGCCGGATC GAAAGAAGAT AACTGAGATT AAATGGCTGG CGGTTTACGA TCTGAGCAGT 
CAAAACAACT TTGGCGACGT TTATATACCC GAGGAGTTCG ATCCACCGAT GAGCCAATTG 
GGCGGAACCT TCTCCAAGCG CAGTCACAAT GTGAGCAGCA GCAGTGTTGA GATCTTGGAC 
TCAAAGACCA TTCGCATCAA GGACTTTACC TACGATGGGC GTGGCAAGAG GACATTTTTC 
TGGACAGGAG TCGGGCCACA GCCATCGAGT CGAGGAAGCA AGCTGCCAGA TGAGCGAGGA 
TACCTTGATC CTATAAGACA GTACAACAAG GAGACCATAG AACTGGAACT GCCAGGAGAT 
AAGACTATCT TTGACATCGA TTGGATCAGT GTATACGATG TGGCTGACAA CGAGAACTAT 
GGTCATGTTC TGTTCAACGA TAAACTTAAT GTTCCACCTT CCCTAGTGAA AGTTACACCA 
TTCGAATTCT CGCTGCCCAA TTGCCGGCAA CTACACAAGG ATATGCAGGT GTCCTGGGAG 
GTTTTCGGGC CGCAGATCAC GTTTCAACTA TCCGGTCAGG TGGGCGGCAA CGATTATATG 
TCTTTCGGCA TATCCGGATC TGATGTATCC AGCCAGATGA TTGGCTCCGA TGTCGTGGTG 
GCCTATATAG ACGACATCAG AGGCTACACC GTGGACTACA ATATCACTTC GTTAGCGCCG 
TGCGTTCAGG TTTTGGGTCA AAACAAAGGA GTTTGCCGTG ACGATGTGGT TGGTGGATTG 
GACAGCTTCC AGTTGAATAC GTATAGTCGC AAGGATGGCA TTAATACGAT CAGTTTTAGG 
CGGACGCTCA AGTCATCCGA TGATGGCGAT AAGGAGATCT TCCTAGACCG CAGCAACTAC 
GTGATCTGGG CATTTGGCCC ATTGGACTCC AACAACGAGC CCGCCTTTCA CACTTACTAT 
CCCAAGAGCG ATATAGTGAT AGATTTTAAC ACCACAGAAC CGGTTAATGA TTGCTTTGCA 
TTTACAAAAC GAGCGGAGAC GACTAATCCT CCAGTGTGGG AGCGAACCAG GATAACAGAT 
GCAACGGTAC GCACATTCAA CGCCTACTTG GGACCCTCTG GAGGACTTCG TGGCTATCAG 
GGATTGACGA ATCATGTGTC CAGTGGCTTG GCCTGGTACA TCAACGGCTA CATGATTCCA 
GAGCTTTACC TAAAGCGTGG TCTGACCTAC ACCTTTAAGG TTAGGGGTGG CAATAATCCT 
CACTCGCCGG AGCACTATCA TCCGTTGGTT ATCACCGATG ATCCCCAGGG TGGGTATGAC 
CGACTGTCTG ATGCAAAGCA GAGTGAGATT CGTGTATTAG CCGGTGTTGA ATTCACCCGG 
AGAGGCAGGC CCAAGCCCAC GGCTGCTGGT CCCCTGTGCC TGTCCAGATA TCCTCAAAAT 
AGTGATCGTC GACTGGATGA TAACTTCCCC ACTTTCAAGA AGTTCAATCG ATCCCTCATC 
ACCGAGTGTG TAGAAGGGGA ACCGGCTCTA TTGGAAATTA CACCGAATAT CACATGGCCG 
GATACTGTGT ACTACAACTC CTTTACCCAT GGCAATATGG GCTGGAAGAT TCACATCGTG 
GACAGTTATA CCAACTTGAA GAGTGGATCG ATGGGCTTAT CCTGGTCCTT GTGCATCATC 
CTGCTACCGT GGCTCGTGCT CCAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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knk cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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