nerfin-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nerfin-1
Entrez Gene ID	44786
Full Name	nervous fingers 1
Synonyms	CG13906,Dmel\CG13906,Nerfin,Nerfin-1,nerfin
General protein information	Preferred Names nervous fingers 1 Names CG13906 gene product from transcript CG13906-RA CG13906-PA Nerfin-1 nerfin nerfin 1 nerfin-1-PA nervous finger-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61D4-61D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_080044.4	NP_524783.1	nervous fingers 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	nerfin-1	NP_524783.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002601	XP_563560.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data revealed a new link to understand the underlying mechanism for Nerfin-1 turnover in post-translational level, and provided useful insights in animal development and disease treatment by manipulating the activity of Slimb and Nerfin-1.
Title: The SCF ubiquitin ligase Slimb controls Nerfin-1 turnover in Drosophila.

Nerfin-1 represses Notch activity in medulla neurons and prevents them from dedifferentiation.
Title: Prevention of medulla neuron dedifferentiation by Nerfin-1 requires inhibition of Notch activity.

(Nerfin-1) locks neurons into differentiation, preventing their reversion into Neuroblasts
Title: The transcription factor Nerfin-1 prevents reversion of neurons into neural stem cells.

EvoPrinter facilitates the discovery and analysis of enhancers that control crucial aspects of nerfin-1 expression.
Title: Conserved sequence block clustering and flanking inter-cluster flexibility delineate enhancers that regulate nerfin-1 expression during Drosophila CNS development.

multiple microRNAs play a cooperative role in the post-transcriptional regulation of nerfin-1 mRNA
Title: The Drosophila nerfin-1 mRNA requires multiple microRNAs to regulate its spatial and temporal translation dynamics in the developing nervous system.

The following nerfin-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nerfin-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20489	NM_080044.4 Latest version!	Drosophila melanogaster nervous fingers 1 (nerfin-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nerfin-1 ( NM_080044.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524783.1 Drosophila melanogaster nervous fingers 1 (nerfin-1), mRNA.

Price & Availability↑

CloneID	OFa20489
Clone ID Related Accession (Same CDS sequence)	NM_080044.4
Accession Version	NM_080044.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nervous fingers 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080044.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCAGA TACAGACACC GATACCGATT CCAGCCCCCG CGGAGCCCAT TGAAAAGCTC 
AGCCACCCGC ACAGCATTGG CATCCAGTTG CAGCACAAGG GGATGCCCCA TGGCCTGGGC 
CTGCTGACCA CAGTGGCCTC GCTGCCCCCC AAGTACCGCA GTCGATATCT GAACCTGAAA 
ACCAGGTGCG AAATACCGCT GGATCTAACG CTGAAGCTTC CCTCACCACT GCCCAGCAGC 
GATGTGCCCA TGGCGGCCAC ATTCGCGGAG GTCTATAGCG AAAATACGAG TGCTAGTTCC 
AGCGAGGTGC AGGGGGAGGT GACCAAGGTG GAGGAGCTGC CGGTGAAGCC CCCAACACCA 
CCGCAGAGTC CAGCAGGGAA GCGTAAATTG AGCCGGGAGA GCTATGACGA CGAGCCGGCT 
AAAATGGCCA AAAAGGAGGA GGAGGTTCCG GTGGAAACAG TAGAATCCAC ACCAGCGAAA 
CCCGTCAAAG TGGCAGAATC TGCGCCTAAA ACCAACAAGG CCTCCACTTC CTCGGGAAAG 
CCCTCCCGCA ATAAAGCCAC CCGCAAGCTG AAGTTCGATG AGGAGACCAG TTCCCCCGTC 
TCGGGCACCG TCATCCGACC GCTCGAGGAC ATCACCGATG GATCGATGCA GTACAGCAAC 
GGCGACATTG ACCCCAAGTA CAACATAGTC GAGATCACGG AGGAGACCAA GGCGGAACTG 
GCCGCCATCA AGAACGTGAT CGGGGACTAT GTCTGCCGAC TGTGCAAGAT CAAGTTCGAG 
GACGCCTTCG GCCTGGCCCG GCACAGGTGC GCCTGCATCG TCCTGCTGGA GTACCGGTGT 
CCCGAGTGCG GCAAGCAGTT CAATTGTCCT GCTAACTTGG CCTCCCATCG CCGGTGGCAC 
AAGCCGAGGA AGGAGGCGTC TAAAAAGGAG AATCGCAATA CCACCAACCA GCCGGAGAAA 
CAGCAGCAGG TGGAGAAAAA GTCGGAGGAG GAACTGGCCT TCGATTGCCA GGAGTGCGGC 
AAGAAGTTTA AGAGAGCCGC CTACCTGAGG AAGCACCAGC TGACCCATCA GAAAAAGGAA 
AAGCCAGCGG AAAAACAGTT GGAAATGAAG CCCACCACCA CAACTATCAC AGGCAGTTTT 
CACTTTAACC AGGCGGTGTC CACCTCCTCC TCCGCCAGCA GTTCGCACTC CGATGAGGGA 
GTCTACGTGG TGGGATCGAA TGCCAGGGAG AACTACGACT ATGACAACGA CTACCTCTCG 
GACTCCAGCT CCTCCGCCTC ATCTGCGGGA TACGGACGGC TTCAGATTGT GGAACATGGT 
CTCACGGAGG AGGAAAGCAT TGCTGCCGCA GCTCTGACCA ACTTGAGGAA CTGCGCCTCC 
GTCATCCAAC ACACAACCAT GGCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524783.1
CDS	187..1596
Translation	MAQIQTPIPIPAPAEPIEKLSHPHSIGIQLQHKGMPHGLGLLTTVASLPPKYRSRYLNLKTRCEIPLDLTLKLPSPLPSSDVPMAATFAEVYSENTSASSSEVQGEVTKVEELPVKPPTPPQSPAGKRKLSRESYDDEPAKMAKKEEEVPVETVESTPAKPVKVAESAPKTNKASTSSGKPSRNKATRKLKFDEETSSPVSGTVIRPLEDITDGSMQYSNGDIDPKYNIVEITEETKAELAAIKNVIGDYVCRLCKIKFEDAFGLARHRCACIVLLEYRCPECGKQFNCPANLASHRRWHKPRKEASKKENRNTTNQPEKQQQVEKKSEEELAFDCQECGKKFKRAAYLRKHQLTHQKKEKPAEKQLEMKPTTTTITGSFHFNQAVSTSSSASSSHSDEGVYVVGSNARENYDYDNDYLSDSSSSASSAGYGRLQIVEHGLTEEESIAAAALTNLRNCASVIQHTTMAH

Target ORF information:

RefSeq Version	NM_080044.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nervous fingers 1 (nerfin-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080044.4

ORF Insert Sequence:

ATGGCCCAGA TACAGACACC GATACCGATT CCAGCCCCCG CGGAGCCCAT TGAAAAGCTC 
AGCCACCCGC ACAGCATTGG CATCCAGTTG CAGCACAAGG GGATGCCCCA TGGCCTGGGC 
CTGCTGACCA CAGTGGCCTC GCTGCCCCCC AAGTACCGCA GTCGATATCT GAACCTGAAA 
ACCAGGTGCG AAATACCGCT GGATCTAACG CTGAAGCTTC CCTCACCACT GCCCAGCAGC 
GATGTGCCCA TGGCGGCCAC ATTCGCGGAG GTCTATAGCG AAAATACGAG TGCTAGTTCC 
AGCGAGGTGC AGGGGGAGGT GACCAAGGTG GAGGAGCTGC CGGTGAAGCC CCCAACACCA 
CCGCAGAGTC CAGCAGGGAA GCGTAAATTG AGCCGGGAGA GCTATGACGA CGAGCCGGCT 
AAAATGGCCA AAAAGGAGGA GGAGGTTCCG GTGGAAACAG TAGAATCCAC ACCAGCGAAA 
CCCGTCAAAG TGGCAGAATC TGCGCCTAAA ACCAACAAGG CCTCCACTTC CTCGGGAAAG 
CCCTCCCGCA ATAAAGCCAC CCGCAAGCTG AAGTTCGATG AGGAGACCAG TTCCCCCGTC 
TCGGGCACCG TCATCCGACC GCTCGAGGAC ATCACCGATG GATCGATGCA GTACAGCAAC 
GGCGACATTG ACCCCAAGTA CAACATAGTC GAGATCACGG AGGAGACCAA GGCGGAACTG 
GCCGCCATCA AGAACGTGAT CGGGGACTAT GTCTGCCGAC TGTGCAAGAT CAAGTTCGAG 
GACGCCTTCG GCCTGGCCCG GCACAGGTGC GCCTGCATCG TCCTGCTGGA GTACCGGTGT 
CCCGAGTGCG GCAAGCAGTT CAATTGTCCT GCTAACTTGG CCTCCCATCG CCGGTGGCAC 
AAGCCGAGGA AGGAGGCGTC TAAAAAGGAG AATCGCAATA CCACCAACCA GCCGGAGAAA 
CAGCAGCAGG TGGAGAAAAA GTCGGAGGAG GAACTGGCCT TCGATTGCCA GGAGTGCGGC 
AAGAAGTTTA AGAGAGCCGC CTACCTGAGG AAGCACCAGC TGACCCATCA GAAAAAGGAA 
AAGCCAGCGG AAAAACAGTT GGAAATGAAG CCCACCACCA CAACTATCAC AGGCAGTTTT 
CACTTTAACC AGGCGGTGTC CACCTCCTCC TCCGCCAGCA GTTCGCACTC CGATGAGGGA 
GTCTACGTGG TGGGATCGAA TGCCAGGGAG AACTACGACT ATGACAACGA CTACCTCTCG 
GACTCCAGCT CCTCCGCCTC ATCTGCGGGA TACGGACGGC TTCAGATTGT GGAACATGGT 
CTCACGGAGG AGGAAAGCAT TGCTGCCGCA GCTCTGACCA ACTTGAGGAA CTGCGCCTCC 
GTCATCCAAC ACACAACCAT GGCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nerfin-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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