robo2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa15208 NM_001272939.2
Latest version!
Drosophila melanogaster roundabout 2 (robo2), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa16433 NM_080531.4
Latest version!
Drosophila melanogaster roundabout 2 (robo2), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa15208
    Clone ID Related Accession (Same CDS sequence) NM_001272939.2
    Accession Version NM_001272939.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product roundabout 2, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001272939.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
    GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC
    AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC
    GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA
    ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG
    ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA
    ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA
    ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT
    GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA
    ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG
    GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA
    ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT
    GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG
    TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT
    GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG
    GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC
    TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG
    GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC
    GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC
    AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT
    CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT
    CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT
    CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC
    AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT
    ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC
    CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT
    GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT
    CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC
    TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA
    GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC
    GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG
    GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT
    AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT
    CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT
    ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG
    CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA
    CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG
    TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG
    GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG
    CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT
    CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA
    TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT
    TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT
    CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC
    AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA
    GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT
    GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC
    GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG
    GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC
    CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC
    GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG
    CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT
    CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG
    CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG
    CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT
    GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC
    AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC
    AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT
    GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA
    CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG
    CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC
    CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC
    ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG
    CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT
    CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG
    CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT
    CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT
    CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG
    GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC
    GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA
    CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG
    AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG
    GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC
    CACAATGTCT GACCTTTGCT CCCTGGTTCA TCCTTGGGCC AGTCTCAGTC CAAATCCCAG
    TCGCAGTCGC AGTCCCAGAG TGATCTGCAG TCCCAGGAAC AACTCTACGC TCGGGAAGTG
    CGGGAATTAC TCGCCTGGTG CGAACGCTTC AACAACGGTG GAGTGGAGAA CTTTGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259868.1
    CDS289..4848
    Translation

    Target ORF information:

    RefSeq Version NM_001272939.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster roundabout 2 (robo2), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
    GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC
    AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC
    GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA
    ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG
    ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA
    ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA
    ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT
    GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA
    ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG
    GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA
    ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT
    GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG
    TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT
    GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG
    GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC
    TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG
    GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC
    GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC
    AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT
    CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT
    CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT
    CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC
    AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT
    ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC
    CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT
    GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT
    CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC
    TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA
    GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC
    GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG
    GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT
    AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT
    CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT
    ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG
    CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA
    CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG
    TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG
    GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG
    CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT
    CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA
    TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT
    TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT
    CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC
    AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA
    GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT
    GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC
    GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG
    GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC
    CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC
    GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG
    CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT
    CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG
    CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG
    CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT
    GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC
    AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC
    AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT
    GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA
    CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG
    CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC
    CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC
    ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG
    CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT
    CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG
    CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT
    CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT
    CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG
    GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC
    GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA
    CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG
    AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG
    GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC
    CACAATGTCT GACCTTTGCT CCCTGGTTCA TCCTTGGGCC AGTCTCAGTC CAAATCCCAG
    TCGCAGTCGC AGTCCCAGAG TGATCTGCAG TCCCAGGAAC AACTCTACGC TCGGGAAGTG
    CGGGAATTAC TCGCCTGGTG CGAACGCTTC AACAACGGTG GAGTGGAGAA CTTTGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16433
    Clone ID Related Accession (Same CDS sequence) NM_080531.4
    Accession Version NM_080531.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product roundabout 2, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_080531.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
    GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC
    AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC
    GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA
    ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG
    ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA
    ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA
    ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT
    GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA
    ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG
    GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA
    ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT
    GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG
    TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT
    GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG
    GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC
    TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG
    GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC
    GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC
    AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT
    CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT
    CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT
    CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC
    AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT
    ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC
    CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT
    GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT
    CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC
    TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA
    GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC
    GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG
    GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT
    AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT
    CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT
    ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG
    CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA
    CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG
    TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG
    GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG
    CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT
    CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA
    TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT
    TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT
    CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC
    AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA
    GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT
    GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC
    GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG
    GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC
    CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC
    GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG
    CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT
    CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG
    CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG
    CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT
    GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC
    AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC
    AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT
    GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA
    CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG
    CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC
    CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC
    ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG
    CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT
    CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG
    CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT
    CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT
    CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG
    GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC
    GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA
    CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG
    AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG
    GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC
    CACAATGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_536792.2
    CDS289..4680
    Translation

    Target ORF information:

    RefSeq Version NM_080531.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster roundabout 2 (robo2), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_080531.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCATTTGT GCGAGATGCG TGGAATGCCG CTGCGGTTGC AACCTCAGTG TTTAACCAAC 
    GAAAGCGAAA TTCAAATTAA AATCAAAAGC AAAATCAAAA ATAAAACTGC CAGCGAGATC
    AAACGCCGAC ACAGAAACAA TCGAGATTTT CATGGGGAGC GGCTAAAAAT AATGCCAGTC
    GATCGGAGTC GACGCGCAGT ATTCCCACTG CTGCTGCTCC TCGCGGGATT AAACGGATTA
    ACTCAAGTCG GAGCGCTCAA GGGTGAAAAT CCACGCATCA TCGAGCATCC CATGGACACG
    ACGGTGCCAA AAAATGATCC ATTTACGTTT AATTGCCAGG CCGAGGGCAA TCCAACACCA
    ACCATTCAAT GGTTTAAGGA CGGTCGCGAA CTGAAGACGG ATACGGGTTC GCATCGCATA
    ATGCTGCCCG CCGGGGGTCT ATTCTTTCTC AAGGTTATCC ACTCACGTAG AGAGAGCGAT
    GCGGGCACTT ACTGGTGCGA GGCCAAAAAC GAGTTTGGAG TGGCACGGTC CAGGAATGCA
    ACGTTGCAAG TGGCATTTCT CCGCGACGAA TTCCGTTTGG AGCCGGCAAA TACCCGCGTG
    GCCCAAGGCG AGGTGGCCCT GATGGAATGC GGTGCCCCCC GAGGATCTCC GGAGCCGCAA
    ATCTCGTGGC GCAAGAACGG CCAGACCCTG AATCTTGTCG GGAACAAGCG GATTCGCATT
    GTCGACGGTG GCAATCTGGC CATCCAGGAA GCCCGCCAAT CGGACGACGG ACGCTACCAG
    TGTGTGGTCA AGAATGTGGT TGGCACCCGG GAGTCGGCCA CCGCTTTTCT TAAAGTGCAT
    GTACGTCCAT TCCTCATCCG AGGACCCCAG AATCAGACGG CGGTGGTGGG CAGCTCGGTG
    GTCTTCCAGT GCCGCATCGG AGGCGATCCC CTGCCTGATG TCCTGTGGCG ACGCACTGCC
    TCCGGCGGCA ATATGCCACT GCGTCGTGTG CACGTACTTG AGGACCGCAG TCTGAAGCTG
    GACGACGTTA CTCTGGAGGA CATGGGCGAG TACACTTGCG AGGCGGACAA TGCGGTGGGC
    GGCATCACGG CCACTGGCAT CCTCACCGTT CACGCTCCCC CCAAATTTGT GATACGCCCC
    AAGAATCAGC TGGTGGAGAT CGGTGATGAA GTGCTGTTCG AGTGCCAAGC GAATGGACAT
    CCCCGACCAA CGCTCTACTG GTCGGTGGAG GGCAACAGCT CCCTGCTGCT CCCCGGCTAT
    CGGGATGGCC GCATGGAAGT GACCCTGACG CCCGAGGGGC GCTCGGTGCT CTCGATAGCT
    CGATTTGCCC GTGAGGATTC CGGAAAGGTG GTCACTTGCA ACGCCCTGAA CGCCGTGGGC
    AGCGTCAGCA GTCGGACTGT GGTCAGTGTG GATACGCAAT TCGAGCTGCC ACCGCCGATT
    ATCGAACAGG GGCCCGTGAA TCAAACGTTG CCCGTTAAAT CAATTGTGGT TCTGCCATGC
    CGAACTCTGG GCACTCCAGT GCCACAGGTC TCTTGGTACC TGGATGGCAT ACCCATCGAT
    GTGCAGGAGC ACGAGCGGCG GAATCTTTCG GACGCTGGAG CCTTAACCAT TTCGGATCTT
    CAGCGCCACG AGGATGAAGG CTTGTACACC TGCGTGGCCA GCAATCGCAA CGGAAAATCC
    TCTTGGAGTG GTTACCTTCG TCTGGACACC CCGACAAATC CGAATATCAA GTTCTTCAGA
    GCCCCAGAAC TTTCCACCTA CCCAGGGCCG CCAGGAAAAC CGCAAATGGT GGAGAAGGGC
    GAAAATTCGG TGACTCTCAG CTGGACGAGG AGCAACAAGG TGGGCGGCTC CAGTCTGGTG
    GGCTATGTAA TCGAGATGTT TGGCAAAAAC GAAACGGATG GCTGGGTGGC TGTGGGCACT
    AGGGTGCAAA ATACCACGTT TACCCAAACG GGTCTGCTGC CGGGTGTGAA TTACTTCTTT
    CTAATTCGAG CCGAGAACTC CCATGGCTTA TCACTGCCCA GTCCGATGTC GGAACCCATT
    ACGGTGGGAA CGCGCTACTT CAATAGTGGT CTGGATCTGA GCGAGGCTCG TGCCAGTCTG
    CTGTCCGGAG ATGTTGTGGA GCTGAGCAAC GCCAGTGTGG TGGACTCCAC TAGCATGAAA
    CTCACCTGGC AGATCATCAA TGGCAAATAC GTCGAGGGCT TCTATGTCTA TGCGAGACAG
    TTGCCAAATC CAATAGTCAA CAATCCGGCG CCCGTTACTA GCAATACCAA TCCGCTGCTG
    GGCTCTACAT CCACATCCGC ATCCGCATCC GCCTCGGCAT CGGCATTGAT TTCGACAAAG
    CCAAATATTG CAGCTGCCGG CAAACGTGAT GGGGAGACAA ACCAGAGTGG AGGAGGAGCT
    CCGACCCCAC TGAACACCAA GTATCGCATG CTAACGATTC TCAATGGCGG TGGCGCCTCA
    TCCTGCACCA TCACCGGGCT CGTCCAGTAC ACGCTGTATG AATTTTTCAT CGTGCCATTT
    TACAAATCCG TCGAGGGCAA GCCGTCGAAT TCGCGCATCG CTCGCACCCT TGAAGATGTT
    CCCTCTGAGG CACCATATGG AATGGAGGCT CTGCTGTTGA ACTCCTCCGC GGTCTTCCTC
    AAATGGAAGG CACCAGAACT CAAGGATCGG CATGGTGTTC TCTTGAACTA TCATGTTATA
    GTCCGAGGTA TTGACACTGC CCACAATTTC TCACGCATTT TGACAAATGT CACCATCGAT
    GCCGCTTCGC CTACTCTGGT TTTGGCCAAT CTCACCGAAG GCGTCATGTA CACCGTGGGC
    GTGGCGGCCG GAAATAACGC TGGAGTTGGT CCTTATTGTG TCCCAGCTAC TTTGCGTTTG
    GATCCCATCA CAAAGCGACT CGATCCGTTC ATCAATCAGC GCTATCCCAT CAATCAGGAC
    CATGTTAACG ATGTGCTGAC GCAGCCCTGG TTCATAATAC TCCTGGGCGC CATCCTGGCC
    GTTCTTATGC TGTCCTTTGG CGCAATGGTC TTTGTGAAGC GCAAGCACAT GATGATGAAG
    CAGTCGGCCC TAAATACAAT GCGTGGCAAT CACACGAGCG ACGTGCTCAA AATGCCGAGT
    CTATCGGCGC GCAATGGAAA CGGCTACTGG CTGGACTCCT CCACCGGCGG AATGGTGTGG
    CGTCCCTCGC CCGGCGGCGA CTCGCTGGAG ATGCAAAAGG ATCACATCGC CGACTATGCG
    CCGGTCTGCG GTGCCCCCGG TTCTCCGGCC GGCGGTGGCA CCTCTTCCGG TGGATCCGGT
    GGCGCGGGCA GCGGTGCCAG CGGCGGCGAT GACATTCATG GAGGACACGG CAGCGAACGC
    AATCAGCAGC GGTACGTGGG CGAGTACTCC AACATACCGA CCGACTATGC AGAGGTGTCC
    AGTTTTGGCA AGGCACCCAG CGAGTATGGT CGGCATGGCA ACGCCTCCCC GGCCCCTTAT
    GCCACCTCTT CGATCCTGAG TCCCCACCAG CAGCAACAGC AGCAGCAGCC GCGTTATCAA
    CAGCGACCAG TGCCCGGCTA TGGGCTCCAG CGCCCAATGC ACCCACACTA CCAGCAGCAG
    CAGCATCAGC AGCAACAGGC GCAGCAGACG CACCAGCAAC ACCAGGCTCT CCAGCAGCAC
    CAGCAACTGC CACCCAGCAA CATCTACCAG CAGATGTCCA CCACCAGCGA GATATACCCC
    ACGAACACGG GTCCTTCGCG CTCTGTCTAC TCTGAGCAGT ATTACTACCC CAAGGACAAG
    CAGAGACACA TCCACATCAC CGAGAACAAG CTGAGCAACT GCCACACCTA TGAGGCGGCT
    CCTGGCGCCA AGCAGTCCTC GCCGATATCC TCGCAGTTCG CCAGCGTGAG GCGGCAGCAG
    CTGCCGCCCA ACTGCAGCAT CGGCAGGGAA AGTGCCCGCT TCAAGGTGCT AAACACGGAT
    CAGGGCAAGA ACCAGCAGAA TCTCCTGGAT CTCGACGGCT CCTCGATGTG CTACAACGGT
    CTGGCAGACT CGGGCTGCGG TGGATCTCCC TCCCCGATGG CCATGCTGAT GTCGCACGAG
    GACGAGCACG CGCTGTACCA CACGGCGGAT GGGGATCTGG ACGACATGGA ACGACTGTAC
    GTCAAGGTGG ACGAGCAGCA GCCTCCACAG CAGCAGCAGC AGCTGATTCC CCTGGTCCCA
    CAGCATCCGG CGGAAGGTCA CCTGCAGTCC TGGCGGAATC AGAGCACGCG GAGCAGTCGG
    AAGAACGGCC AGGAATGCAT CAAGGAACCC AGCGAGTTGA TCTACGCTCC GGGAAGCGTG
    GCCAGCGAAC GGAGCCTCCT CAGCAACTCG GGTAGCGGCA CCAGCAGCCA GCCAGCTGGC
    CACAATGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.