pwn cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following pwn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pwn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa11312 NM_136423.2
Latest version!
Drosophila melanogaster pawn (pwn), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa12206 NM_001273834.1
Latest version!
Drosophila melanogaster pawn (pwn), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa11312
    Clone ID Related Accession (Same CDS sequence) NM_136423.2
    Accession Version NM_136423.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pawn, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136423.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGACGAA AGCCACAGAA CTTAACACAC TCTATACCAT CGAGTAGAGC CGGAGACAGA 
    GGCGGAGGAG GAGGAGGAGT AGGTCGGGGC ACCTCCCGGC CGTCAGCAAT TTCCAACAAT
    AGAAGGCCGA GAGACCATCG ACACTTGATG GCCATGGTCG TGTCGACGTT GCCACTGGCT
    CTTTTGCTGC TCCTCGTTCA AACAACAGCT GGCCAGCACG AGAGCGTCTC ACAGATCTCA
    CCCAAATCGA GCAGCCACCG CAGTCCGGAC CGTCAGAGGA TCGAGAGCAC CATGGCGGAC
    TCCGTAAACG GTCTGGCCGA AGGCCAATCA TCGCCCCTGG ATGTGAACGA TAACGAAACG
    CGGGCGGAGC GAGTGGAGCG CTCGGCGTCG CCCATTCTGC ATGATCGGCA GCCAATCGGA
    CCCAACGATG TGCACTTCCC TGAGGATCTG GAAAAGGACG TGTCCGCCGG ACGGTACTTC
    CACTACAACA TCAAGCCCAC GGGCACCTTC GACAACCAGG ATGAGCAGCC GGAGCGTACG
    CACCAGGGGA TCAGGGCTGG GAAGACCCTG TCGCAAGGTG GCAGCAGCTC AGAGCAGTCT
    TTTTTAGGGC GGGGGGACCT GCGACCGCTA GTGTCGGGGT CCCCGATTCG GCCGAGTCCG
    AGCAATACTG CCACCTCAGC TACGGCAGCC AGTGCCACCC AGGCGCCGCA TAGTCCGCGC
    CAGAACGGAA ACCCCGACAT CCAGGACATC ATCACAGGCA TCGTCAAGCT GCTGAATGGC
    AATGTCAATG TCCATGCTAA TACGCAGGGC ATTAGGCGTC CCTCGGCCAG TAGGATTAAC
    AATCGCGGCC CACCGCGGAT CTCCGAGGCC CAGAACCTGC CAATCGACTA CGAGGCTCAG
    AAGCCGGGCA CTTCCATGCG CCCGCCGCCA TATCCCTTCG ATCGTCCGGA ACGACCCTTC
    ATTACCGGCG TGCCTATTCC CGAGCAGATT GTACCCGTAC GACCGGGATT CGTGAGCAAT
    CGTCCGCCCT GGTACCGCAA CAAGCCACGC CCACCTATTG CCACCAGTGT GGGCGGCAAT
    CGGAGACCCC TGCCCCAATA TAAGCCTCTT CCAGCTCCTC CAGTGCAAGC GCCTCTTCAG
    CCGCAGGATC CACTGGATAG AAAGAACGAA CAGGAGAAGG AGCAGAATCA TCAGGAGCAG
    CCGCAGCCAA CTGATGATGT TGTGCTGCCC ACCGACACCA CATACGACTC GGAGTTCTCC
    AATGAAGATG CCAATGCCCA GTACATCGAA GTGTCCGATC AGGATACGGA TGCCACTGGC
    GGAGAAGTAG AAGACGAACT GCAGCCGCCT CCTCCTCCAC CGTCCACAAC CAACAATCCA
    CCAACTACAC CAACAAATCC CTCAAAGAAA AAGCACAAGC CAAAGCCAGG AAGCGAGAAA
    AAGAAGCTAC CAGAGGAGTC TCCTCAGGAT GAGGGCTTCA GCACCATTAT TGAGACCAGT
    TCCGTGGTGC ACACCGTGAT GGGCATGTCC TCCACTTATG TGCCCATGTC CATGGACAGT
    TCGGAGAGCC TTGGTCTGGA TCCCTCCACC GAGGAGGTGA TCTTCATGAC CGCCAATCGA
    ACGCCCACTT TAGAACCAAG TGTCACATCC TCGCTGGGCA CCTCCTCGTC CTCCAACCCG
    GCCTCAGCCA TTCAACCTAC GTCCTCCAAA GAACATACCA AAACCACCAC TACCACTACT
    ACTACCACCA GCAGCTCCAC CTCAACCACA ACCTCAACCA CTACCCCGCC ATCTACTGAA
    AGTGTGTCGC CTAATAGTTC CAATGCTAAC ACCAATGCCA CTCCGCCCGC CCCCTACCAT
    CCCCGTCCTG GGATAGTCCT CGATGATCCC GAGTTTAAGC CCGGCGGTCG GCCACGCCCA
    CCTGTCCAGC GACCACCCGC CCAGCAGACC CTTCCCTTGC CAGCGGTGCA GCCCACTCGC
    CAGCATCTGC CCCCCGGCTA CGGGGAGATC TTCGACGTCA CCCTGTCCGC CATCCAGGGA
    CCGGGTCCCA AGGGCAGTGG ATCCCAGCAG ACGATCAACA TTAAGCCATA TGGAAGCTAT
    GCGGCGGGCG GAGGTCAGGG CGACATCATC GTGTCGGCAT CAGGTGACGA CGGCTTTGTC
    TCGATCGACG GCAAGCGCAC TTACATCAAT CTCTTTGGTG ACCCCACAGA TCCTCCAGCG
    GGCGCCACAA CTCCAGCCAC GCGCCTTCCC CAGTTGCCGG GCTCCGGATC AGGATCAGGT
    TCAGGATCAG GTACAGGTTC AGGAGGTGCA TCCACGCCGG GAGTTGGCGT CAGCAGTGGT
    GGTGGTGGTG GTAGTGGTTC CTCCATCTCT CCTGCTGCTC CTGCCAACGC TGTCACCCAG
    AGCAGTGGTG GCTACGTTGT GCCCGAAACG GAGGTGGTGG ACCTACAGGG GCACAGCAAG
    CCCCAAGGCG GAGCGCCCAC AACGAATGCC GGACCCACGA GGCCTCACTA CCGCCAGAGA
    CCAACGCAGC CGCCGGTGAG GATTGACACC TGTATCGTGG GCGATGATTC CACCTGCGAT
    CAGGCGCAGC ACGAGCGATG CAAGACGGAC AACGGCGTTT CCAGCTGTCA CTGCAGACCA
    GGATACTCCC GGCGAAAGCA TCGGGAGCCA TGCCGGCGGG TCATCTCATT CCATCTGGGC
    ATGCGCGTAG ATCGCATTTA CGAGCACCGA ATTGTGTGGG ACACCAAGCT CATGGACAAA
    CACAGCGAAC CCTTTGGACA ACTTAGCTAT GAATCAATAA GAGCGCTGGA CTCTGCCATG
    TCCATGACGC CCTACTCGGA TGAGTTCATG GAGGCGAAGG TGAACAACAT CTACCGGGGC
    GATCCTAATC TGGGCGGCAG CGGCGTCTAC GTGAACATGA CCATCAAGCT GGATGAAAGT
    GTAGAAACAT TGCGACCCAA TCTGCGCAGT GACGTTCAGA AGCATCTTCT GGGTGTGCTC
    CATCGGCGGA ACAACAACAT CGGCAACTCC GTGCTGTACG TGAGCTCCCC GGAGGGCGCA
    GTGTCGGCTC TGCAGGATCT GGATGAGTGT CAGTCACCGG AGCTAAATGA CTGCCATTCC
    GGCGCCAGTT GCAGCAACAC CTGGGGCAGC TTCCGCTGCG CCTGCGAGGC GGGACTCCGC
    GATCCGTGGG CTGACCAGCC GGCGCGGTCT GGACGCGAGT GCCAGGCCTG CGCCGACTCC
    GTCTGCAACA ACCACGGTAC CTGCAGCTAC GCCGAGGATG GTGCTCAGCT GTGCACCTGC
    GACTCCAGTC ACTATGGCGC CCAGTGTGAA ATCGACGGCG AGGTGTTGGG CGTGGCTATC
    GGCGCCTCCG TGGCGGCCAT TATCATCATC GTCCTGACCC TGGTGTGCCT GATCATGTGG
    TCGCGGCGCT GGCAGCGAGA GCAGAAGAAC GCCATGGGTT CACCTGTGTT TGGATATATG
    AACACTGCAC CCCTGAAGTC CGCCGGTTTG CCACAGGCGG GTTACCAGGT GACCCTGGAG
    GACCGCATGC GCTGGGCCCA GATAGCCGAT GTCATGGCGC AAACGAATCA CTACGGGGCT
    GAGCCCATCG GACCCACTAG ACCCTCGTCG GCGATGTTTG CCTACCCGAA CTTGGGCGCC
    ATGGGAATGG GCACCCTGGG CGGGATGTCT CTGCAGAGCA CCATGCAGAT GCACCCCAGT
    GCCACTCTGG CTCCTCCAGT GCCATTGCCC AGACTTGGCC TTGGTCCCCG CTCGAATGGA
    ATGCGCACAC TGGAGAACTC CAGTTCCAGC GAGGAGGAGG ATCGCGCCGA TTTGCTGGGA
    CGCAACTTCC AAGTGCCACG TCCCAAGAGT CGCAGCAATG GCAGCATAGC GAACCAATCT
    GGCATCTACT ACGATGTGGA CTACGAGCCG TCCGGGAATG GGATCGGCAA CTCCAGCGTG
    GACCACCTGT ACGGGTCGCA AAATCAGTCC GTGACGCACT CATCAGGCCA CAGCCACATC
    CCGGGACCCC AGGGCATCCC GATGAGCACG TACACCTCCG GGCGAGCTCC CAGCAGCTAC
    TACATGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_610267.2
    CDS1309..5400
    Translation

    Target ORF information:

    RefSeq Version NM_136423.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pawn (pwn), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_136423.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGACGAA AGCCACAGAA CTTAACACAC TCTATACCAT CGAGTAGAGC CGGAGACAGA 
    GGCGGAGGAG GAGGAGGAGT AGGTCGGGGC ACCTCCCGGC CGTCAGCAAT TTCCAACAAT
    AGAAGGCCGA GAGACCATCG ACACTTGATG GCCATGGTCG TGTCGACGTT GCCACTGGCT
    CTTTTGCTGC TCCTCGTTCA AACAACAGCT GGCCAGCACG AGAGCGTCTC ACAGATCTCA
    CCCAAATCGA GCAGCCACCG CAGTCCGGAC CGTCAGAGGA TCGAGAGCAC CATGGCGGAC
    TCCGTAAACG GTCTGGCCGA AGGCCAATCA TCGCCCCTGG ATGTGAACGA TAACGAAACG
    CGGGCGGAGC GAGTGGAGCG CTCGGCGTCG CCCATTCTGC ATGATCGGCA GCCAATCGGA
    CCCAACGATG TGCACTTCCC TGAGGATCTG GAAAAGGACG TGTCCGCCGG ACGGTACTTC
    CACTACAACA TCAAGCCCAC GGGCACCTTC GACAACCAGG ATGAGCAGCC GGAGCGTACG
    CACCAGGGGA TCAGGGCTGG GAAGACCCTG TCGCAAGGTG GCAGCAGCTC AGAGCAGTCT
    TTTTTAGGGC GGGGGGACCT GCGACCGCTA GTGTCGGGGT CCCCGATTCG GCCGAGTCCG
    AGCAATACTG CCACCTCAGC TACGGCAGCC AGTGCCACCC AGGCGCCGCA TAGTCCGCGC
    CAGAACGGAA ACCCCGACAT CCAGGACATC ATCACAGGCA TCGTCAAGCT GCTGAATGGC
    AATGTCAATG TCCATGCTAA TACGCAGGGC ATTAGGCGTC CCTCGGCCAG TAGGATTAAC
    AATCGCGGCC CACCGCGGAT CTCCGAGGCC CAGAACCTGC CAATCGACTA CGAGGCTCAG
    AAGCCGGGCA CTTCCATGCG CCCGCCGCCA TATCCCTTCG ATCGTCCGGA ACGACCCTTC
    ATTACCGGCG TGCCTATTCC CGAGCAGATT GTACCCGTAC GACCGGGATT CGTGAGCAAT
    CGTCCGCCCT GGTACCGCAA CAAGCCACGC CCACCTATTG CCACCAGTGT GGGCGGCAAT
    CGGAGACCCC TGCCCCAATA TAAGCCTCTT CCAGCTCCTC CAGTGCAAGC GCCTCTTCAG
    CCGCAGGATC CACTGGATAG AAAGAACGAA CAGGAGAAGG AGCAGAATCA TCAGGAGCAG
    CCGCAGCCAA CTGATGATGT TGTGCTGCCC ACCGACACCA CATACGACTC GGAGTTCTCC
    AATGAAGATG CCAATGCCCA GTACATCGAA GTGTCCGATC AGGATACGGA TGCCACTGGC
    GGAGAAGTAG AAGACGAACT GCAGCCGCCT CCTCCTCCAC CGTCCACAAC CAACAATCCA
    CCAACTACAC CAACAAATCC CTCAAAGAAA AAGCACAAGC CAAAGCCAGG AAGCGAGAAA
    AAGAAGCTAC CAGAGGAGTC TCCTCAGGAT GAGGGCTTCA GCACCATTAT TGAGACCAGT
    TCCGTGGTGC ACACCGTGAT GGGCATGTCC TCCACTTATG TGCCCATGTC CATGGACAGT
    TCGGAGAGCC TTGGTCTGGA TCCCTCCACC GAGGAGGTGA TCTTCATGAC CGCCAATCGA
    ACGCCCACTT TAGAACCAAG TGTCACATCC TCGCTGGGCA CCTCCTCGTC CTCCAACCCG
    GCCTCAGCCA TTCAACCTAC GTCCTCCAAA GAACATACCA AAACCACCAC TACCACTACT
    ACTACCACCA GCAGCTCCAC CTCAACCACA ACCTCAACCA CTACCCCGCC ATCTACTGAA
    AGTGTGTCGC CTAATAGTTC CAATGCTAAC ACCAATGCCA CTCCGCCCGC CCCCTACCAT
    CCCCGTCCTG GGATAGTCCT CGATGATCCC GAGTTTAAGC CCGGCGGTCG GCCACGCCCA
    CCTGTCCAGC GACCACCCGC CCAGCAGACC CTTCCCTTGC CAGCGGTGCA GCCCACTCGC
    CAGCATCTGC CCCCCGGCTA CGGGGAGATC TTCGACGTCA CCCTGTCCGC CATCCAGGGA
    CCGGGTCCCA AGGGCAGTGG ATCCCAGCAG ACGATCAACA TTAAGCCATA TGGAAGCTAT
    GCGGCGGGCG GAGGTCAGGG CGACATCATC GTGTCGGCAT CAGGTGACGA CGGCTTTGTC
    TCGATCGACG GCAAGCGCAC TTACATCAAT CTCTTTGGTG ACCCCACAGA TCCTCCAGCG
    GGCGCCACAA CTCCAGCCAC GCGCCTTCCC CAGTTGCCGG GCTCCGGATC AGGATCAGGT
    TCAGGATCAG GTACAGGTTC AGGAGGTGCA TCCACGCCGG GAGTTGGCGT CAGCAGTGGT
    GGTGGTGGTG GTAGTGGTTC CTCCATCTCT CCTGCTGCTC CTGCCAACGC TGTCACCCAG
    AGCAGTGGTG GCTACGTTGT GCCCGAAACG GAGGTGGTGG ACCTACAGGG GCACAGCAAG
    CCCCAAGGCG GAGCGCCCAC AACGAATGCC GGACCCACGA GGCCTCACTA CCGCCAGAGA
    CCAACGCAGC CGCCGGTGAG GATTGACACC TGTATCGTGG GCGATGATTC CACCTGCGAT
    CAGGCGCAGC ACGAGCGATG CAAGACGGAC AACGGCGTTT CCAGCTGTCA CTGCAGACCA
    GGATACTCCC GGCGAAAGCA TCGGGAGCCA TGCCGGCGGG TCATCTCATT CCATCTGGGC
    ATGCGCGTAG ATCGCATTTA CGAGCACCGA ATTGTGTGGG ACACCAAGCT CATGGACAAA
    CACAGCGAAC CCTTTGGACA ACTTAGCTAT GAATCAATAA GAGCGCTGGA CTCTGCCATG
    TCCATGACGC CCTACTCGGA TGAGTTCATG GAGGCGAAGG TGAACAACAT CTACCGGGGC
    GATCCTAATC TGGGCGGCAG CGGCGTCTAC GTGAACATGA CCATCAAGCT GGATGAAAGT
    GTAGAAACAT TGCGACCCAA TCTGCGCAGT GACGTTCAGA AGCATCTTCT GGGTGTGCTC
    CATCGGCGGA ACAACAACAT CGGCAACTCC GTGCTGTACG TGAGCTCCCC GGAGGGCGCA
    GTGTCGGCTC TGCAGGATCT GGATGAGTGT CAGTCACCGG AGCTAAATGA CTGCCATTCC
    GGCGCCAGTT GCAGCAACAC CTGGGGCAGC TTCCGCTGCG CCTGCGAGGC GGGACTCCGC
    GATCCGTGGG CTGACCAGCC GGCGCGGTCT GGACGCGAGT GCCAGGCCTG CGCCGACTCC
    GTCTGCAACA ACCACGGTAC CTGCAGCTAC GCCGAGGATG GTGCTCAGCT GTGCACCTGC
    GACTCCAGTC ACTATGGCGC CCAGTGTGAA ATCGACGGCG AGGTGTTGGG CGTGGCTATC
    GGCGCCTCCG TGGCGGCCAT TATCATCATC GTCCTGACCC TGGTGTGCCT GATCATGTGG
    TCGCGGCGCT GGCAGCGAGA GCAGAAGAAC GCCATGGGTT CACCTGTGTT TGGATATATG
    AACACTGCAC CCCTGAAGTC CGCCGGTTTG CCACAGGCGG GTTACCAGGT GACCCTGGAG
    GACCGCATGC GCTGGGCCCA GATAGCCGAT GTCATGGCGC AAACGAATCA CTACGGGGCT
    GAGCCCATCG GACCCACTAG ACCCTCGTCG GCGATGTTTG CCTACCCGAA CTTGGGCGCC
    ATGGGAATGG GCACCCTGGG CGGGATGTCT CTGCAGAGCA CCATGCAGAT GCACCCCAGT
    GCCACTCTGG CTCCTCCAGT GCCATTGCCC AGACTTGGCC TTGGTCCCCG CTCGAATGGA
    ATGCGCACAC TGGAGAACTC CAGTTCCAGC GAGGAGGAGG ATCGCGCCGA TTTGCTGGGA
    CGCAACTTCC AAGTGCCACG TCCCAAGAGT CGCAGCAATG GCAGCATAGC GAACCAATCT
    GGCATCTACT ACGATGTGGA CTACGAGCCG TCCGGGAATG GGATCGGCAA CTCCAGCGTG
    GACCACCTGT ACGGGTCGCA AAATCAGTCC GTGACGCACT CATCAGGCCA CAGCCACATC
    CCGGGACCCC AGGGCATCCC GATGAGCACG TACACCTCCG GGCGAGCTCC CAGCAGCTAC
    TACATGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12206
    Clone ID Related Accession (Same CDS sequence) NM_001273834.1
    Accession Version NM_001273834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product pawn, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGACGAA AGCCACAGAA CTTAACACAC TCTATACCAT CGAGTAGAGC CGGAGACAGA 
    GGCGGAGGAG GAGGAGGAGT AGGTCGGGGC ACCTCCCGGC CGTCAGCAAT TTCCAACAAT
    AGAAGGCCGA GAGACCATCG ACACTTGATG GCCATGGTCG TGTCGACGTT GCCACTGGCT
    CTTTTGCTGC TCCTCGTTCA AACAACAGCT GGCCAGCACG AGAGCGTCTC ACAGATCTCA
    CCCAAATCGA GCAGCCACCG CAGTCCGGAC CGTCAGAGGA TCGAGAGCAC CATGGCGGAC
    TCCGTAAACG GTCTGGCCGA AGGCCAATCA TCGCCCCTGG ATGTGAACGA TAACGAAACG
    CGGGCGGAGC GAGTGGAGCG CTCGGCGTCG CCCATTCTGC ATGATCGGCA GCCAATCGGA
    CCCAACGATG TGCACTTCCC TGAGGATCTG GAAAAGGACG TGTCCGCCGG ACGGTACTTC
    CACTACAACA TCAAGCCCAC GGGCACCTTC GACAACCAGG ATGAGCAGCC GGAGCGTACG
    CACCAGGGGA TCAGGGCTGG GAAGACCCTG TCGCAAGGTG GCAGCAGCTC AGAGCAGTCT
    TTTTTAGGGC GGGGGGACCT GCGACCGCTA GTGTCGGGGT CCCCGATTCG GCCGAGTCCG
    AGCAATACTG CCACCTCAGC TACGGCAGCC AGTGCCACCC AGGCGCCGCA TAGTCCGCGC
    CAGAACGGAA ACCCCGACAT CCAGGACATC ATCACAGGCA TCGTCAAGCT GCTGAATGGC
    AATGTCAATG TCCATGCTAA TACGCAGGGC ATTAGGCGTC CCTCGGCCAG TAGGATTAAC
    AATCGCGGCC CACCGCGGAT CTCCGAGGCC CAGAACCTGC CAATCGACTA CGAGGCTCAG
    AAGCCGGGCA CTTCCATGCG CCCGCCGCCA TATCCCTTCG ATCGTCCGGA ACGACCCTTC
    ATTACCGGCG TGCCTATTCC CGAGCAGATT GTACCCGTAC GACCGGGATT CGTGAGCAAT
    CGTCCGCCCT GGTACCGCAA CAAGCCACGC CCACCTATTG CCACCAGTGT GGGCGGCAAT
    CGGAGACCCC TGCCCCAATA TAAGCCTCTT CCAGCTCCTC CAGTGCAAGC GCCTCTTCAG
    CCGCAGGATC CACTGGATAG AAAGAACGAA CAGGAGAAGG AGCAGAATCA TCAGGAGCAG
    CCGCAGCCAA CTGATGATGT TGTGCTGCCC ACCGACACCA CATACGACTC GGAGTTCTCC
    AATGAAGATG CCAATGCCCA GTACATCGAA GTGTCCGATC AGGATACGGA TGCCACTGGC
    GGAGAAGTAG AAGACGAACT GCAGCCGCCT CCTCCTCCAC CGTCCACAAC CAACAATCCA
    CCAACTACAC CAACAAATCC CTCAAAGAAA AAGCACAAGC CAAAGCCAGG AAGCGAGAAA
    AAGAAGCTAC CAGAGGAGTC TCCTCAGGAT GAGGGCTTCA GCACCATTAT TGAGACCAGT
    TCCGTGGTGC ACACCGTGAT GGGCATGTCC TCCACTTATG TGCCCATGTC CATGGACAGT
    TCGGAGAGCC TTGGTCTGGA TCCCTCCACC GAGGAGGTGA TCTTCATGAC CGCCAATCGA
    ACGCCCACTT TAGAACCAAG TGTCACATCC TCGCTGGGCA CCTCCTCGTC CTCCAACCCG
    GCCTCAGCCA TTCAACCTAC GTCCTCCAAA GAACATACCA AAACCACCAC TACCACTACT
    ACTACCACCA GCAGCTCCAC CTCAACCACA ACCTCAACCA CTACCCCGCC ATCTACTGAA
    AGTGTGTCGC CTAATAGTTC CAATGCTAAC ACCAATGCCA CTCCGCCCGC CCCCTACCAT
    CCCCGTCCTG GGATAGTCCT CGATGATCCC GAGTTTAAGC CCGGCGGTCG GCCACGCCCA
    CCTGTCCAGC GACCACCCGC CCAGCAGACC CTTCCCTTGC CAGCGGTGCA GCCCACTCGC
    CAGCATCTGC CCCCCGGCTA CGGGGAGATC TTCGACGTCA CCCTGTCCGC CATCCAGGGA
    CCGGGTCCCA AGGGCAGTGG ATCCCAGCAG ACGATCAACA TTAAGCCATA TGGAAGCTAT
    GCGGCGGGCG GAGGTCAGGG CGACATCATC GTGTCGGCAT CAGATCCTCC AGCGGGCGCC
    ACAACTCCAG CCACGCGCCT TCCCCAGTTG CCGGGCTCCG GATCAGGATC AGGTTCAGGA
    TCAGGTACAG GTTCAGGAGG TGCATCCACG CCGGGAGTTG GCGTCAGCAG TGGTGGTGGT
    GGTGGTAGTG GTTCCTCCAT CTCTCCTGCT GCTCCTGCCA ACGCTGTCAC CCAGAGCAGT
    GGTGGCTACG TTGTGCCCGA AACGGAGGTG GTGGACCTAC AGGGGCACAG CAAGCCCCAA
    GGCGGAGCGC CCACAACGAA TGCCGGACCC ACGAGGCCTC ACTACCGCCA GAGACCAACG
    CAGCCGCCGG TGAGGATTGA CACCTGTATC GTGGGCGATG ATTCCACCTG CGATCAGGCG
    CAGCACGAGC GATGCAAGAC GGACAACGGC GTTTCCAGCT GTCACTGCAG ACCAGGATAC
    TCCCGGCGAA AGCATCGGGA GCCATGCCGG CGGGTCATCT CATTCCATCT GGGCATGCGC
    GTAGATCGCA TTTACGAGCA CCGAATTGTG TGGGACACCA AGCTCATGGA CAAACACAGC
    GAACCCTTTG GACAACTTAG CTATGAATCA ATAAGAGCGC TGGACTCTGC CATGTCCATG
    ACGCCCTACT CGGATGAGTT CATGGAGGCG AAGGTGAACA ACATCTACCG GGGCGATCCT
    AATCTGGGCG GCAGCGGCGT CTACGTGAAC ATGACCATCA AGCTGGATGA AAGTGTAGAA
    ACATTGCGAC CCAATCTGCG CAGTGACGTT CAGAAGCATC TTCTGGGTGT GCTCCATCGG
    CGGAACAACA ACATCGGCAA CTCCGTGCTG TACGTGAGCT CCCCGGAGGG CGCAGTGTCG
    GCTCTGCAGG ATCTGGATGA GTGTCAGTCA CCGGAGCTAA ATGACTGCCA TTCCGGCGCC
    AGTTGCAGCA ACACCTGGGG CAGCTTCCGC TGCGCCTGCG AGGCGGGACT CCGCGATCCG
    TGGGCTGACC AGCCGGCGCG GTCTGGACGC GAGTGCCAGG CCTGCGCCGA CTCCGTCTGC
    AACAACCACG GTACCTGCAG CTACGCCGAG GATGGTGCTC AGCTGTGCAC CTGCGACTCC
    AGTCACTATG GCGCCCAGTG TGAAATCGAC GGCGAGGTGT TGGGCGTGGC TATCGGCGCC
    TCCGTGGCGG CCATTATCAT CATCGTCCTG ACCCTGGTGT GCCTGATCAT GTGGTCGCGG
    CGCTGGCAGC GAGAGCAGAA GAACGCCATG GGTTCACCTG TGTTTGGATA TATGAACACT
    GCACCCCTGA AGTCCGCCGG TTTGCCACAG GCGGGTTACC AGGTGACCCT GGAGGACCGC
    ATGCGCTGGG CCCAGATAGC CGATGTCATG GCGCAAACGA ATCACTACGG GCAGGCTGAG
    CCCATCGGAC CCACTAGACC CTCGTCGGCG ATGTTTGCCT ACCCGAACTT GGGCGCCATG
    GGAATGGGCA CCCTGGGCGG GATGTCTCTG CAGAGCACCA TGCAGATGCA CCCCAGTGCC
    ACTCTGGCTC CTCCAGTGCC ATTGCCCAGA AGACTTGGCC TTGGTCCCCG CTCGAATGGA
    ATGCGCACAC TGGAGAACTC CAGTTCCAGC GAGGAGGAGG ATCGCGCCGA TTTGCTGGGA
    CGCAACTTCC AAGTGCCACG TCCCAAGAGT CGCAGCAATG GCAGCATAGC GAACCAATCT
    GGCATCTACT ACGATGTGGA CTACGAGCCG TCCGGGAATG GGATCGGCAA CTCCAGCGTG
    GACCACCTGT ACGGGTCGCA AAATCAGTCC GTGACGCACT CATCAGGCCA CAGCCACATC
    CCGGGACCCC AGGGCATCCC GATGAGCACG TACACCTCCG GGCGAGCTCC CAGCAGCTAC
    TACATGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260763.1
    CDS518..4549
    Translation

    Target ORF information:

    RefSeq Version NM_001273834.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster pawn (pwn), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGACGAA AGCCACAGAA CTTAACACAC TCTATACCAT CGAGTAGAGC CGGAGACAGA 
    GGCGGAGGAG GAGGAGGAGT AGGTCGGGGC ACCTCCCGGC CGTCAGCAAT TTCCAACAAT
    AGAAGGCCGA GAGACCATCG ACACTTGATG GCCATGGTCG TGTCGACGTT GCCACTGGCT
    CTTTTGCTGC TCCTCGTTCA AACAACAGCT GGCCAGCACG AGAGCGTCTC ACAGATCTCA
    CCCAAATCGA GCAGCCACCG CAGTCCGGAC CGTCAGAGGA TCGAGAGCAC CATGGCGGAC
    TCCGTAAACG GTCTGGCCGA AGGCCAATCA TCGCCCCTGG ATGTGAACGA TAACGAAACG
    CGGGCGGAGC GAGTGGAGCG CTCGGCGTCG CCCATTCTGC ATGATCGGCA GCCAATCGGA
    CCCAACGATG TGCACTTCCC TGAGGATCTG GAAAAGGACG TGTCCGCCGG ACGGTACTTC
    CACTACAACA TCAAGCCCAC GGGCACCTTC GACAACCAGG ATGAGCAGCC GGAGCGTACG
    CACCAGGGGA TCAGGGCTGG GAAGACCCTG TCGCAAGGTG GCAGCAGCTC AGAGCAGTCT
    TTTTTAGGGC GGGGGGACCT GCGACCGCTA GTGTCGGGGT CCCCGATTCG GCCGAGTCCG
    AGCAATACTG CCACCTCAGC TACGGCAGCC AGTGCCACCC AGGCGCCGCA TAGTCCGCGC
    CAGAACGGAA ACCCCGACAT CCAGGACATC ATCACAGGCA TCGTCAAGCT GCTGAATGGC
    AATGTCAATG TCCATGCTAA TACGCAGGGC ATTAGGCGTC CCTCGGCCAG TAGGATTAAC
    AATCGCGGCC CACCGCGGAT CTCCGAGGCC CAGAACCTGC CAATCGACTA CGAGGCTCAG
    AAGCCGGGCA CTTCCATGCG CCCGCCGCCA TATCCCTTCG ATCGTCCGGA ACGACCCTTC
    ATTACCGGCG TGCCTATTCC CGAGCAGATT GTACCCGTAC GACCGGGATT CGTGAGCAAT
    CGTCCGCCCT GGTACCGCAA CAAGCCACGC CCACCTATTG CCACCAGTGT GGGCGGCAAT
    CGGAGACCCC TGCCCCAATA TAAGCCTCTT CCAGCTCCTC CAGTGCAAGC GCCTCTTCAG
    CCGCAGGATC CACTGGATAG AAAGAACGAA CAGGAGAAGG AGCAGAATCA TCAGGAGCAG
    CCGCAGCCAA CTGATGATGT TGTGCTGCCC ACCGACACCA CATACGACTC GGAGTTCTCC
    AATGAAGATG CCAATGCCCA GTACATCGAA GTGTCCGATC AGGATACGGA TGCCACTGGC
    GGAGAAGTAG AAGACGAACT GCAGCCGCCT CCTCCTCCAC CGTCCACAAC CAACAATCCA
    CCAACTACAC CAACAAATCC CTCAAAGAAA AAGCACAAGC CAAAGCCAGG AAGCGAGAAA
    AAGAAGCTAC CAGAGGAGTC TCCTCAGGAT GAGGGCTTCA GCACCATTAT TGAGACCAGT
    TCCGTGGTGC ACACCGTGAT GGGCATGTCC TCCACTTATG TGCCCATGTC CATGGACAGT
    TCGGAGAGCC TTGGTCTGGA TCCCTCCACC GAGGAGGTGA TCTTCATGAC CGCCAATCGA
    ACGCCCACTT TAGAACCAAG TGTCACATCC TCGCTGGGCA CCTCCTCGTC CTCCAACCCG
    GCCTCAGCCA TTCAACCTAC GTCCTCCAAA GAACATACCA AAACCACCAC TACCACTACT
    ACTACCACCA GCAGCTCCAC CTCAACCACA ACCTCAACCA CTACCCCGCC ATCTACTGAA
    AGTGTGTCGC CTAATAGTTC CAATGCTAAC ACCAATGCCA CTCCGCCCGC CCCCTACCAT
    CCCCGTCCTG GGATAGTCCT CGATGATCCC GAGTTTAAGC CCGGCGGTCG GCCACGCCCA
    CCTGTCCAGC GACCACCCGC CCAGCAGACC CTTCCCTTGC CAGCGGTGCA GCCCACTCGC
    CAGCATCTGC CCCCCGGCTA CGGGGAGATC TTCGACGTCA CCCTGTCCGC CATCCAGGGA
    CCGGGTCCCA AGGGCAGTGG ATCCCAGCAG ACGATCAACA TTAAGCCATA TGGAAGCTAT
    GCGGCGGGCG GAGGTCAGGG CGACATCATC GTGTCGGCAT CAGATCCTCC AGCGGGCGCC
    ACAACTCCAG CCACGCGCCT TCCCCAGTTG CCGGGCTCCG GATCAGGATC AGGTTCAGGA
    TCAGGTACAG GTTCAGGAGG TGCATCCACG CCGGGAGTTG GCGTCAGCAG TGGTGGTGGT
    GGTGGTAGTG GTTCCTCCAT CTCTCCTGCT GCTCCTGCCA ACGCTGTCAC CCAGAGCAGT
    GGTGGCTACG TTGTGCCCGA AACGGAGGTG GTGGACCTAC AGGGGCACAG CAAGCCCCAA
    GGCGGAGCGC CCACAACGAA TGCCGGACCC ACGAGGCCTC ACTACCGCCA GAGACCAACG
    CAGCCGCCGG TGAGGATTGA CACCTGTATC GTGGGCGATG ATTCCACCTG CGATCAGGCG
    CAGCACGAGC GATGCAAGAC GGACAACGGC GTTTCCAGCT GTCACTGCAG ACCAGGATAC
    TCCCGGCGAA AGCATCGGGA GCCATGCCGG CGGGTCATCT CATTCCATCT GGGCATGCGC
    GTAGATCGCA TTTACGAGCA CCGAATTGTG TGGGACACCA AGCTCATGGA CAAACACAGC
    GAACCCTTTG GACAACTTAG CTATGAATCA ATAAGAGCGC TGGACTCTGC CATGTCCATG
    ACGCCCTACT CGGATGAGTT CATGGAGGCG AAGGTGAACA ACATCTACCG GGGCGATCCT
    AATCTGGGCG GCAGCGGCGT CTACGTGAAC ATGACCATCA AGCTGGATGA AAGTGTAGAA
    ACATTGCGAC CCAATCTGCG CAGTGACGTT CAGAAGCATC TTCTGGGTGT GCTCCATCGG
    CGGAACAACA ACATCGGCAA CTCCGTGCTG TACGTGAGCT CCCCGGAGGG CGCAGTGTCG
    GCTCTGCAGG ATCTGGATGA GTGTCAGTCA CCGGAGCTAA ATGACTGCCA TTCCGGCGCC
    AGTTGCAGCA ACACCTGGGG CAGCTTCCGC TGCGCCTGCG AGGCGGGACT CCGCGATCCG
    TGGGCTGACC AGCCGGCGCG GTCTGGACGC GAGTGCCAGG CCTGCGCCGA CTCCGTCTGC
    AACAACCACG GTACCTGCAG CTACGCCGAG GATGGTGCTC AGCTGTGCAC CTGCGACTCC
    AGTCACTATG GCGCCCAGTG TGAAATCGAC GGCGAGGTGT TGGGCGTGGC TATCGGCGCC
    TCCGTGGCGG CCATTATCAT CATCGTCCTG ACCCTGGTGT GCCTGATCAT GTGGTCGCGG
    CGCTGGCAGC GAGAGCAGAA GAACGCCATG GGTTCACCTG TGTTTGGATA TATGAACACT
    GCACCCCTGA AGTCCGCCGG TTTGCCACAG GCGGGTTACC AGGTGACCCT GGAGGACCGC
    ATGCGCTGGG CCCAGATAGC CGATGTCATG GCGCAAACGA ATCACTACGG GCAGGCTGAG
    CCCATCGGAC CCACTAGACC CTCGTCGGCG ATGTTTGCCT ACCCGAACTT GGGCGCCATG
    GGAATGGGCA CCCTGGGCGG GATGTCTCTG CAGAGCACCA TGCAGATGCA CCCCAGTGCC
    ACTCTGGCTC CTCCAGTGCC ATTGCCCAGA AGACTTGGCC TTGGTCCCCG CTCGAATGGA
    ATGCGCACAC TGGAGAACTC CAGTTCCAGC GAGGAGGAGG ATCGCGCCGA TTTGCTGGGA
    CGCAACTTCC AAGTGCCACG TCCCAAGAGT CGCAGCAATG GCAGCATAGC GAACCAATCT
    GGCATCTACT ACGATGTGGA CTACGAGCCG TCCGGGAATG GGATCGGCAA CTCCAGCGTG
    GACCACCTGT ACGGGTCGCA AAATCAGTCC GTGACGCACT CATCAGGCCA CAGCCACATC
    CCGGGACCCC AGGGCATCCC GATGAGCACG TACACCTCCG GGCGAGCTCC CAGCAGCTAC
    TACATGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.