wb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wb
Entrez Gene ID	43946
Full Name	wing blister
Synonyms	&agr1,2,2Lan,2]],A1,BEST:CK02229,BG:DS03792.1,BG:DS03792.2,CG15288,CG32970,CG42677,CK02229,CT35236,DLAM,Dmel\CG42677,Dmel_CG15288,Dmel_CG32970,LM-A/alpha2,LanA1,alpha[[1,fus5,l(2)09437,l(2)34Fb,l(2)br1,l34Fb
General protein information	Preferred Names wing blister Names CG42677 gene product from transcript CG42677-RB CG42677-PB CG42677-PC CG42677-PD CG42677-PE CG42677-PF CG42677-PG D-laminin alpha2 SGP cluster fusion defects 5 alpha laminin laminin laminin A2 laminin alpha1,2 laminin alpha[[1,2]] lamininW lethal 1 in the black-reduced region lethal group A1 wb-PB wb-PC wb-PD wb-PE wb-PF wb-PG wing blistered
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35A3-35A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165086.3	NP_723870.1	wing blister, isoform B
NM_001201877.2	NP_001188806.1	wing blister, isoform C
NM_001201879.2	NP_001188808.1	wing blister, isoform E
NM_001201878.2	NP_001188807.1	wing blister, isoform D
NM_057442.5	NP_476790.3	wing blister, isoform G
NM_001201880.2	NP_001188809.1	wing blister, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	wb	NP_723870.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007849	XP_555667.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wb laminin is an extracellular matrix ligand that is essential for integrin-dependent cellular migration in Drosophila.
Title: Tissue-specific laminin expression facilitates integrin-dependent association of the embryonic wing disc with the trachea in Drosophila.

The following wb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15182	NM_165086.3 Latest version!	Drosophila melanogaster wing blister (wb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa15183	NM_057442.5 Latest version!	Drosophila melanogaster wing blister (wb), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa15831	NM_001201880.2 Latest version!	Drosophila melanogaster wing blister (wb), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa15831	NM_001201877.2 Latest version!	Drosophila melanogaster wing blister (wb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa15831	NM_001201879.2 Latest version!	Drosophila melanogaster wing blister (wb), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa15831	NM_001201878.2 Latest version!	Drosophila melanogaster wing blister (wb), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wb ( NM_165086.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723870.1 Drosophila melanogaster wing blister (wb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15182
Clone ID Related Accession (Same CDS sequence)	NM_165086.3
Accession Version	NM_165086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wing blister, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165086.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAATCG GAAATTTTAT CAAATGGCCA CTGGGCCTGA TCCTCATCCT GCTGGTCACC 
CACTGTCAGG CCAAAAGGCA GCGGAAACGG TTGCGTGGAT TCTCCACTGC CAAGGACCTA 
CAGGATTATA GCATGCCGCT CAACGACAAC TCCACGGAAC TCCAGCAGAA CATTGTGTCC 
TGGACAGGAG CTCGAATTGC GTCGAGTCCC GATAAGAAGA AGAAGTCACC GCAATCATCG 
AGACGTCACA AAACCAAAAG TGGCCTGATC ATTGAAAGTA ACATTGCCGA CTCGGAATCG 
ATGTTGGCTA TTGCCTCCTC GGAATCCGTC ACCAAGAAGA AGCCGCCGCA GCGTCGTCGC 
AACGGAAAAT TACAGAAATC CGGAGGAGGA GCAGGAGGTG GAGCCCTTCG ATTGCTCAAA 
ACGGAGGACG TGTACTCATC CAGTTTCTCC GGCGGCCTCT ATCCGCCGCT CTTCAATGTG 
GTTCCACGGG CTCAGATCTC GGTGAATGCC ACTTGTGGCC AGAACGGTGC CGAGGAGTAC 
TGCAAACAGG TCGGCGCCAA GCCGTGTGGC ATCTGCAATG CCCATTCCTC GGATCGAGCC 
AAACAGCGCT CCATACAGTC GCTGATATCG TCGGGATCGG GATCGGGATC GGGATCGGGA 
TTTGAAGAAG GCTGGTGGCA ATCACCAACG CTGCAGGGTG GCCGGCAATT TGAGTATGTG 
ACCATACTAT TGGATCTGAA GCAGACTTTT CAAATATTTT CCGTGTGGCT AAAATCGGCC 
AACTCTCCGC GTCCAGCCTC ATGGATACTG GAAAAGTCCC TGGATGGGAT TAACTTTGAG 
CCATGGCAGT ATTTCGGATT GAGCGATGCA GATTGTCAGC GACGATGGAA TTTATCCGGG 
CAAAATGGCA AATACGTCTT CCAGAACGAT ACAGAAATCA TTTGCTCCAC TCAGTTTTCG 
AAGCCGGGTC CTTTGGAGAA CGGCGTCTTG CATGCTTCGC TGCTGAAGAA TCGCCCTGGA 
GCCACGGATC AAAGTCCGGA GCTAATGAAG TTCATAACGA CTCGGTACAT AAGGATTCGC 
CTGCAGGGCA TGCACTCCAC GGCCAATCAG GACAACAGTC TGGACTGGCT GCTGGACTCT 
CCGTCGCTGG AGAAGCACAG CTTCTACTCG CTGTCCCAGT TGAAGGTGAG TGCCCGTCTG 
GATTGTAATG GGCACGCCAA TCGCTCCCAC GAGTCGCCAG ATGACCCTCT GATGCAGTGC 
ATCTGCCAGC ACAATACCTG TGGTGCTCAG TGCGAACAGT GCTGTCCGCT TTTCCAGGAT 
CGCCCCTATC AAATGGGCGG AGAGTGCGAG ATATGCCAAT GCTATGGTCA CGCCGAGAGC 
TGCACCTATG ATCCCTTCCT GGACAAGGGC ATTTGCCAGA GCTGCTCGAA TAACACGGCT 
GGCATTGAGT GCGAGTTTTG CGAGATGGGA TTTTACAGGG AGCTGGATGC ACCGCTCACC 
GATCCCTGTT TGCCCTGCTC CTGTAATCCT GCTCGTTCCA CGGGTGGTTG CCAGTCGGAT 
GGAGGAAGCT GTAACTGCTT GGAGGGTTTC CAGGGCAAGA ACTGCGAGGA ATGTGCTCCG 
GGCTACTACG GAGACGATTG CAAACGGTGT GAGTGCGACG AAAGGGGAAG TCTTGGCTCC 
ACGGGTTCCT GCTCGGGTGT GTGCCAGTGC AAGCTCAATG TGGAGGGCAG CACCTGCTCC 
GAGTGTGCGC CCGGATATTT TGATCTAAGC GCAGAGAATG CAGAGGGATG CACCAGCTGT 
TGGTGCTCAG GTGTCTCGCA AACATGTCAC AGTGCCAAAC TGCAGACATT GGCCTTTGAA 
ACCCTCAACG ATTGGAAAAT AACCGATATT CAAAGAGTGA AACCAATTTC AATACCAGTT 
GATGCCGAAA CAAACCGTTT GATCTTTGCC AACGAACTGG ATGAAGTGGA GGCCATTTAC 
TGGCAGGCTT CGTTGGGTTA CTTGGGAAAT CGCTTGACCA GCTATGGGTC GAGACTGCAG 
CTGGTGTTGT CGTGGGATGT GATAAGAGGC GATAGATCGG GAAAGCCCAC GACGGGGCCC 
AATGTCATCC TAGTCGGCAA GAATGGACTG AAGATTGCCT TCGGCGATGA ATCCTTGGAC 
GGATTGGGAA TCAACTTGAA TGTAACCCTA ACAGAGGTTG GCTGGTATCA CGTGCCACCC 
ACTGTCGTTG ATATAAAAAC AAGACTTCGG CGAACCGAGG GCGGTGACTA TCACGGAGAA 
TCTGTTACCC GTTCCCAGTT TCTCTCCGTT CTCGTTTCCC TCGATGCTGT CCTTATTAGA 
GCGGCTTTTC ACACGGATCA AGGGGAAACA TCACTCGAGA GAGCAGTCAT CTATTCAGGA 
GGTGTGGAGT TGGGCGGAAA GTCTTCCAGC CAAGTGGAGC AATGCCTCTG CCCAGCTGGC 
TATACCGGTC TGTCCTGCGA GGGCTGCGCC TTTGGATTCA AGCGGATTTA CGAGAACACC 
TCGGACCATC AGATTCTGAG CAAGTGCATC CCGTGTCCCT GCAACGGACA CTCAAACTCC 
TGCGATCTGC AGAGCGGAAA TTGCGGCGAC TGCATGCACA ACACTTTTGG AGATCGTTGT 
GAGCGTTGCC AGTTGGGTTA CTACGGGAAT CCCCTGCAGG GAACCCCCAA TGACTGCAAG 
CGATGCGCCT GTCCCCTGTC CGAGGATTCC AACAACTTCT CGCCCAGTTG TCAGCTAAAG 
AGCTACAACT ACATGGACCT GAATCCGCAG TTCGAGCTGA TAGAGCATGC GGAGTACATA 
TGCACCCAAT GTCCGGAAGG TTATACGGGT GATCATTGTC AGGTGTGCGA CGATGGGTAC 
TTCGGGAATC CACGCCAGCC GGGAAGCAGT TGTCAGCGCT GCGATTGCGC CGGAGGGCCC 
TGCAACGTGA CCACCGGGGA GTGCATCACG TGCCGCGGAA ACACAGAGGG TTGGCACTGC 
GAGCGCTGCA AGCTGGGCTA CTGGGGTGAT CCGGCCGTGG GCTGTGATCC CTGTCATTGC 
CACACGGAAG GATCGGAGAG CGGACTATGC GACAGCACCG ATGGTCAATG CCTGTGTAAG 
CCACGTTATG CTGGACAGAA GTGCGATGAA TGTGACGTGG GCTATGCTAA TGTTGAGCTA 
CGCTGTCCAT CCTGCAACTG CGATCCATTG GGCTCGTTGG TCCAGGACAG ATGTGATCCC 
CACACTGGAC AATGCCATTG TAAGGAGGGC GTTATGGGTG CCAAATGCCA CGAGTGCCAG 
GATGGCTACT TCGGCATGAA TGCTGTGGCC TATCGAATGG ATGACCTTGC TGCACTGCGT 
CAAAACTCCG ATAGCGACGA TGACGAATGG GAATTGGTTC CAGATACTGA AGATCCCAAT 
AGCGAATCCA CGGTGGCCTG CGAGGAATGC CACTGCAGCA GTGTGGGATC CCTGTCATCG 
GATTGCGATA AGCGAACTGG GCAATGCGCC TGCCTGGCCA ACGTCACCGG GAGGAGATGC 
GACAAGTGTC GACCGGGTCA CTGGAATCTC ACGGCGGGCG AGGGCTGCCG GGATTGCCGA 
TGCGATCCTC ATGGTTCCCG AGGTCACGAG TGCAACCCGT GGACGGGCCA ATGCGACTGC 
AAGATCGGGG TTGGAGGTCA GCACTGTAAC GAGTGTACGG AGGGATTCTT TGGCTTCAGC 
ACGGAGGGTT GTCAGCGGTG CTCGGCCTGC AGGTCGGAGG GTCAGGTGTG TGACCCGCAC 
AATGGACGCT GCATTTGCCC CAAGTTCACC CGTGGCCTGG GATGTGGACA GTGTGTGCCG 
GGCACCTGGG GCTGGCAGGC TCGATTGGGT TGCCGGGAAT GCGAGTGCGA TCACATTGGC 
TCCATTGGTC AGCAGTGCAG CACCGGCGAT GGACAATGCC AGTGCCGAGA GGGATATTCG 
GGCAGAAAGT GCGACACTTG CGCCATCGGG TATTTCGGCT ATCCGGAGTG CCGACGATGC 
GGCTGCGATG CGGAGGGTTC CTTCACCCAG GCGGATGGCT CAATAGCCTG TGACTCGAAT 
GGCCAATGCC CGTGCAAATC CCTTGTGGTG GGTCTCAAAT GCGATACCTG CATGCAAAGC 
ACTTTTGGAT TGTCCGCACA AAATCCCGAA GGATGCACCC GATGCTTCTG CTTCGGGAGA 
TCCGGTGAGT GCGAGCAGAG CGACTTGTCT TGGGGACACA TCCGTATGGC GGAGTCCAGA 
AATCTCAGCG TTCAGCAAAT AAGACCGCAC TCGGTACCCA GCTCAGACTA CGAGTACATA 
GTGGTGGTTC AGATGGAGGG CAGCAGTTTC CATCGCGAAG ACGCGGAGAT CCAGCGCATG 
AATGATCTGA GCTTAGTGCC CAAATCTACG GGGAATGTTT CCATCGGAGC ATATGGACAA 
TTTTACCATC CGCTCTACTT CCAACTGCCT CCGCAGTTCT ACGGAGATCG GACTAGTAGC 
TATGGTGGCT TTTTGTACTT CACCCTGATC ACCGAAGGTG CCCACAAGCC CTTGGAGCGA 
AATATTTTGG GTCAGTATCC ATTGGTTCAG TTACACGCGC ACTCCAAGTT GCTACTGGAC 
TTTTACGAGT ACGAAGAGTT CGAGTACTCC TTGAACGTGA CCCATCGGGT GCCGCTGCAT 
GAGTCCTTCT GGAAGTACCA CCACACCAGC CAGGCAGTGG ATCGTAATAC TCTAATGGCT 
GCCCTCCAAA ACATTCGACA CATCTTCATT CGAGCCTTTG CTTTTGCCGA TTTCCAGGAA 
GTTGTCCTTC AAAATGTACA CATGGATGCG GCCATCTATA TCAAGGGCTC CACCAATCTT 
ATAGCCAAGG GAGTCGAACG GTGTAAGTGT CCCAAGCGGT TTGATGGACT TTCATGCCAG 
GATCCGGGAA GATCCTTCTA CCGTTGGCGG AATACCACTA TTGTGGAGAG TGTATTCATT 
GAGGATCTGA TAGGAAGAGC AGCTCCCTGT CATTGTAATG GTCGTAGCAG CGATTGTGAT 
CGCGAAACTG GAGTCTGTCA GAACTGTCGA GGTAACACTG GAGGAGATCA CTGCCATCAG 
TGTGCTGAGG GATTCTACGG CGATCCCAAT TCTCCACACG GTTGTCAGGC TTGTCCGTGC 
CCGGAGACCA ATAGGAACTT TGCCCGGGGC TGCAATGTCT GGGACGGAGA AGTCAGTTGT 
GTCTGCAAGC CGGGTTACAC GGGCAGATTG TGTGAGCGGT GCCAGGCGGG ATATTTTGGA 
GATCCTATGA GATATCCAAA CACTACTTGC CAACCTTGCA ACTGTCACCC AGATGGAATC 
CAAACCGAAG GCTGCGATGT AGAGACTGGT AGATGCTACT GTCGAGAAGG AGTTACTGGG 
CTAAAGTGCG ACAAGTGTCA GGCGGAACGT CATCATCTGG TGGACAACGG ATGCAAAATC 
TGTGACAATT GCACTCTTCT GCTTCTGGAT TATATGGAGC TGGTGGGCAA CAAGCTTCGT 
CGTGGAATGC ACAACATGGA TTTGACTGGA ATTCCGGCGC CATATCGAAA ATTGTCAGAG 
TACGAAAGCG CCTATGAGAA ATGGAATGGG CGCCACTGGG ACTTTAGCCA GACCAAACGG 
CGTCTACAGG ATTACGACTC CGCTGACATC CTAAAGCTGG AGGCCCACGC CGAAAATTTG 
AAGTTTCAAT CCCGCAAAGC AGTGGCCACC ATTGGAAAAC GGGAGTTTGC CATAAAATCG 
ATGCGGGAAG ATGCTGTAAC CCAACAGCAT AGTGTCGGCT TACTCCGCTC GGAGATTCTT 
CAAACCCTTT CCGATTTACA TGGCTATGGA AAGAGTGCCC ATTATCTCAG TCTGCCTACT 
GCTCTCAAGC AGGCCCGATT CTACCTGCAG GCCATCCGGG AGCACGACCA AATGGTTCAG 
GGAATCCGCA GTACCAACGA CTGTGCATGG AAGCATTTTT ATGCCATGGG CAACGCATCG 
GATGCGTCGT TCGACGAGAG CGGCCGCTTG GAGATGCTCT GGCGGGACTT GAATCAGACC 
AATCATCGAG TGGTAGACAT GCGGCTGCAG GTGGATCGCG TGCAAGAGGT GGAGAACGAA 
GCGGAGGACG TTCTGGAACA TGTGCGCAAT TTGAGTATTC GCGTGGGTGA ATCCCACCAG 
GAGCTGGATG AACTTAACCA GCGTATTTCC GATCACCTAG ATCCGGGCTA CCTAGAACAG 
GGCGAGGGCC TGCTAAGGCT GACAGTCCAG CGGCAGATAA TGCTCAATGG GCATCTTAAC 
CAATTGGATG GCTACCGTAT ACTACTAAAT ACCACATTGG GCGTAAAGAC TGAGCAGCAG 
CGGGAGGTGC GTAAACACTG GCTTCCCAAG GCGGAAAAGC ACGCCAGTCA TCTGCTGGCG 
CGATCCAATG AGTACGCCCG CAAGTTTCAA CCCACCAGAA ATGGAGCCCG CATAGCCATG 
CTGGCCAGTT CGGCCCACAG CAATATCACT GAGGCCATCA ACGATGCCCG GCTAGCATCA 
ATCCTGGCCA AGGAACGGGT GTACGAGGCG CAGAGAACAC TCTATCCCAG TGACGGCAGC 
TCCATGATCG AGCGAGCCAA GCACTCGCTG CACAGATCAA AGCAACTTCA GCAAGAAGCG 
CTCAAGCAGA TGCACAAATC CAATGTGTTG AAGGACAAAC TGCATCGCCA GGAGCAGCAG 
GTGGAGGGCA TCAAGTCCAC GATTTACGAT TCCGGACTGC GCACCAACAA CATATCCGGA 
CAGCTGCAGG GTCTTTCAGA TGGTTCAGCA CGTCGTCAAG CGAAGGATAG TCTGGAAATG 
GCTGATAGAA CGGGAGAACA AATGCGTGCT GAACTGCAAA AGGCGAAGGA TATGCAGAAG 
TCCATCCAAA ACATGCGAAA TTCGTTCTCC AATCTGGAAC CCGACTGGGA AATCAAGCTG 
GGAATGGCCC AAGAGAACAT ATCCCTGACC CAGACTAACT TGCGTCTGGC CAATGTCTCA 
TTGAGCTACC TAGAGCAGCA GGCCGAGAAG GAGCAACAGG TGTTCGAGGT GTGGAACAAC 
AGCATGGCCC AGCAACTGCA GCAGCTCCGG GATCAAATAG CCAAGGCCAG ACATGCAGCA 
GAAGCGATCG ATGTTTCCCT GGAATCGCTG GGTCCTAAAT GCATTCGATC CTATTTGCCC 
GCCTCCTACG GTTTGAGTAC CTCGAATAAG ATTCGTATGA GCTTCGCCTT ATCCAATCAC 
CTCGAGAGCT CTCCATTGAT TCACCTGGCC AGCAGCGAGG GGCGTCACAT CACCCTGGAG 
CTGTACAAAC GTCGTGTGCG CCTGGTGTGG AATCTTGGTG GCACCACCGC AACCGTTACG 
CATCCCATGG TGGTGCATAC TCGTGACCCC AAGTACGACG ATGCCTGGTA CCATGTGGAG 
GCCAATAGGA CACTTAATCT GGGCAGCCTG GTCGTGCGTC GAATGAACAA CTATGGCGAG 
CTGACTCCGC CTAACCCGGT CATCATAACA GGATCCACGG ATACGGAGCA CACACGTTTC 
TATCAGTCTC GAAGCGATCG AATTTCGCTG GGAGGCTTTG CATCGAAAGA TTTGCAATTC 
ACGCCGGGAC TCAATGTGGT GGTGCATCAG GTGGAGGTGG ACAACAAACC GCTGGGTCTG 
TGGAACTTCG TGACCAGCGA GGGCAGCTGC GGTGGATCAA TGGTTGGAGC TAAGGAGTCT 
TCGGCCTCGT CCACGGCTCG CCATTTCAAT GGATTAGGCT ATGCCCAGTT GATGAAGACC 
CGACCACGAC CCACTCGCAA AAACCTGTTC TCCGTGCAGA TGACTTTCCG CACGTTGGAC 
GAAAATGCCT TGCTCTTCTT GGCGGTTGAT GACAAGAACA ACCGCTCGGT ATCGGTAACT 
TTGAGCCGGG GTCGGATCAT GTTCCGCATT GACTATGGCG ATGAGTCCAA GCTGGAAATC 
AACACCACTA AGAAGTATAA TGTGGGCCAG TGGATCAAGA TCGAGGCTGC GCGAGAGTTC 
TCGGCGAAAA GAAGTACTGA AAATGGAATG CTCCGAGTAA ACAACGATCG TCCAATTTCC 
GGAGCACCTA CACTACCCGT AAACATTCAC TTGCTTCCGG ATCTTTCCAA GACTGTCTAT 
TATCTGGGCG GTGTGCCACC AGGTTTCACT TCGGGAACCT CAAAGGCGCC TGGTGCTGAT 
AATCCCTTCT TGGGATGTAT GAAGGATGTC CAGGTAAATG GCGAGACCTA CGATCCTCTG 
GAGAGCTCAA GCTACTACGG TGTGGAGCCT TCCTGCAAGA ACATGATTAC CAAGGCTGGT 
TTCTCGGGCA ATGGTTACTT GGAGCTGCCA TCGCAGTCAC TACGAAAGCG CTCCAATACC 
GCTTTGGTGT TCCGCACCCT TCAGCCCGAT TGCCTGCTGC TCCTTGCTGC CTATCCGCCT 
GAGATCCTGG GCGACTATGA TGCTAAGGAC ATTAAGGGCA ACTTCTCCAT TAGCCTGGTC 
GATGGTCAAT TGCATGTTTG GGTTAACTCT GGTCGCAGCT TCATCAAGAT GTCCTCGAAC 
TCCAGTCAGA TGAACGATGG CGAGTTTCAC GTGGTACATT TGATCAAGAC CGGTCGCAAG 
CTGGAGCTGA TGGTGGACGA TGAGCTGCAA GAGATTCGCA ATCTAAACGG CAATCCCACG 
GTGGTCAGCC TGCCGAGGGA TGCAGGTGGC CTGTATATAG GTGGAGCTCC GCCGCACGAA 
AGTTATACGC CATTGGCGCC GACTTTTGTA AATCTAGAAG GAGCCATTCG GGATGTGGTG 
TTCAACAATC GGACCATTAA CTTTAACGAT GCCCTGACCT TTGCCAACGT CCAGATTGGA 
CGTAATGGTC CACTGATGGG CAGTCTGAAA GGAGGACTGT ACGATGTCCT CCTGAAAACC 
GAACCCATGA TTGGAAAGAG TTTTACGGCC TCGCCTGAGG GCTGCAAGCG GATCGGCAGC 
TATTCCTACG AGCCGAATGC ATTCAAGTTT GGAGACGATA TCTATAGCTA TTCGCAGCTG 
AAGCTACCGG AGCGTCATTT CTGGCAGCGC AACTTCCATC TGAGCTTCGA TTTCCGCTCC 
TTCTATCCCA ATGGAATGCT CTACTTGTCG CCGGGAAGCA AGGAGAAACC CAAGCACTAC 
GTGGCTCTCG TCCTCAAGGA CGGACAGCTG GTCCTGGTGG TGCGAGGTCG TCGGCGCGAG 
GAACTCCAGT TGACCGCCAA GTTAAACGAT GGCGAATGGC ACCGGGTGAC GATTAGCTGT 
CACGATCGCA AGGTCACCAT GTCGGTGGAA ATAGGACGTA CAGATCAAAA GACTTCCGCC 
CAGATGAAGC TGCCCAAGAA GATTGGCGCC TCTCAGCTTT TGCTGGTTGG TGGGCTACCA 
CAGTCTCCAG TCAAGGTCTC CTCCGATCTC TATGTCCGTC TGGAACCATT CAAGGGTTGT 
CTGCGCCGGG TGAGCATCAA CAACAACACC CAGGATCTGG CCCGACCCGG CAAACATTCG 
AATGTGGGTC AGTGCTTTCC CACGGTGGAG AGAGGCAGTT ACTTTCCCGG CGATGCCTAT 
GCCATTTACA AGAAAAACTT CAATGTGGGC AAGTATCTCG ATCTGGAAAC GGAGTTTAGG 
ACCTCGGAGC TATCGGGCAT TCTGCTGAGT GTCTCTGATC CAAATGGATT CCCAGCGCTT 
TCACTGGAAC TGCATAATGG CAATATAATA TTCTCTTGCG ATCCTGGAAA CGGAGCGCCC 
ATGCGTGTGG AGTCATCACT GCCCACCAAA TATGCCCTAT GTGACAACAA ATGGCATAAT 
ATTTCAGCTC TCTACGATGG CGAACAGATT GTCCTGCGCA TCGATCAATT GCCAGCCGTG 
ATCAGTGTGG GTAATCAGGG GAATGCTGGA AAGGTGCAAA CCCGATCCCC GCTCTATATT 
GGCGGTTTGC CAGAGAGTGC TCCCAGTGGT TCACTGATAT CGCGGGAGAA CTTCAAGGGC 
TGCATCCGGC ACGTGTCCAT ACGGAACGAG AGACGCGACT GGATCGAGAT GGAGGACCTG 
AGGAACGTGC TGCTCAGCGA GTGCCTCGTG TCCAGCGATG AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723870.1
CDS	695..10822
Translation	MQIGNFIKWPLGLILILLVTHCQAKRQRKRLRGFSTAKDLQDYSMPLNDNSTELQQNIVSWTGARIASSPDKKKKSPQSSRRHKTKSGLIIESNIADSESMLAIASSESVTKKKPPQRRRNGKLQKSGGGAGGGALRLLKTEDVYSSSFSGGLYPPLFNVVPRAQISVNATCGQNGAEEYCKQVGAKPCGICNAHSSDRAKQRSIQSLISSGSGSGSGSGFEEGWWQSPTLQGGRQFEYVTILLDLKQTFQIFSVWLKSANSPRPASWILEKSLDGINFEPWQYFGLSDADCQRRWNLSGQNGKYVFQNDTEIICSTQFSKPGPLENGVLHASLLKNRPGATDQSPELMKFITTRYIRIRLQGMHSTANQDNSLDWLLDSPSLEKHSFYSLSQLKVSARLDCNGHANRSHESPDDPLMQCICQHNTCGAQCEQCCPLFQDRPYQMGGECEICQCYGHAESCTYDPFLDKGICQSCSNNTAGIECEFCEMGFYRELDAPLTDPCLPCSCNPARSTGGCQSDGGSCNCLEGFQGKNCEECAPGYYGDDCKRCECDERGSLGSTGSCSGVCQCKLNVEGSTCSECAPGYFDLSAENAEGCTSCWCSGVSQTCHSAKLQTLAFETLNDWKITDIQRVKPISIPVDAETNRLIFANELDEVEAIYWQASLGYLGNRLTSYGSRLQLVLSWDVIRGDRSGKPTTGPNVILVGKNGLKIAFGDESLDGLGINLNVTLTEVGWYHVPPTVVDIKTRLRRTEGGDYHGESVTRSQFLSVLVSLDAVLIRAAFHTDQGETSLERAVIYSGGVELGGKSSSQVEQCLCPAGYTGLSCEGCAFGFKRIYENTSDHQILSKCIPCPCNGHSNSCDLQSGNCGDCMHNTFGDRCERCQLGYYGNPLQGTPNDCKRCACPLSEDSNNFSPSCQLKSYNYMDLNPQFELIEHAEYICTQCPEGYTGDHCQVCDDGYFGNPRQPGSSCQRCDCAGGPCNVTTGECITCRGNTEGWHCERCKLGYWGDPAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELRCPSCNCDPLGSLVQDRCDPHTGQCHCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQARFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQTNHRVVDMRLQVDRVQEVENEAEDVLEHVRNLSIRVGESHQELDELNQRISDHLDPGYLEQGEGLLRLTVQRQIMLNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEKHASHLLARSNEYARKFQPTRNGARIAMLASSAHSNITEAINDARLASILAKERVYEAQRTLYPSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISGQLQGLSDGSARRQAKDSLEMADRTGEQMRAELQKAKDMQKSIQNMRNSFSNLEPDWEIKLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEVWNNSMAQQLQQLRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHLASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAWYHVEANRTLNLGSLVVRRMNNYGELTPPNPVIITGSTDTEHTRFYQSRSDRISLGGFASKDLQFTPGLNVVVHQVEVDNKPLGLWNFVTSEGSCGGSMVGAKESSASSTARHFNGLGYAQLMKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGRIMFRIDYGDESKLEINTTKKYNVGQWIKIEAAREFSAKRSTENGMLRVNNDRPISGAPTLPVNIHLLPDLSKTVYYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSYYGVEPSCKNMITKAGFSGNGYLELPSQSLRKRSNTALVFRTLQPDCLLLLAAYPPEILGDYDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQEIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFNNRTINFNDALTFANVQIGRNGPLMGSLKGGLYDVLLKTEPMIGKSFTASPEGCKRIGSYSYEPNAFKFGDDIYSYSQLKLPERHFWQRNFHLSFDFRSFYPNGMLYLSPGSKEKPKHYVALVLKDGQLVLVVRGRRREELQLTAKLNDGEWHRVTISCHDRKVTMSVEIGRTDQKTSAQMKLPKKIGASQLLLVGGLPQSPVKVSSDLYVRLEPFKGCLRRVSINNNTQDLARPGKHSNVGQCFPTVERGSYFPGDAYAIYKKNFNVGKYLDLETEFRTSELSGILLSVSDPNGFPALSLELHNGNIIFSCDPGNGAPMRVESSLPTKYALCDNKWHNISALYDGEQIVLRIDQLPAVISVGNQGNAGKVQTRSPLYIGGLPESAPSGSLISRENFKGCIRHVSIRNERRDWIEMEDLRNVLLSECLVSSDES

Target ORF information:

RefSeq Version	NM_165086.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wing blister (wb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165086.3

ORF Insert Sequence:

ATGCAAATCG GAAATTTTAT CAAATGGCCA CTGGGCCTGA TCCTCATCCT GCTGGTCACC 
CACTGTCAGG CCAAAAGGCA GCGGAAACGG TTGCGTGGAT TCTCCACTGC CAAGGACCTA 
CAGGATTATA GCATGCCGCT CAACGACAAC TCCACGGAAC TCCAGCAGAA CATTGTGTCC 
TGGACAGGAG CTCGAATTGC GTCGAGTCCC GATAAGAAGA AGAAGTCACC GCAATCATCG 
AGACGTCACA AAACCAAAAG TGGCCTGATC ATTGAAAGTA ACATTGCCGA CTCGGAATCG 
ATGTTGGCTA TTGCCTCCTC GGAATCCGTC ACCAAGAAGA AGCCGCCGCA GCGTCGTCGC 
AACGGAAAAT TACAGAAATC CGGAGGAGGA GCAGGAGGTG GAGCCCTTCG ATTGCTCAAA 
ACGGAGGACG TGTACTCATC CAGTTTCTCC GGCGGCCTCT ATCCGCCGCT CTTCAATGTG 
GTTCCACGGG CTCAGATCTC GGTGAATGCC ACTTGTGGCC AGAACGGTGC CGAGGAGTAC 
TGCAAACAGG TCGGCGCCAA GCCGTGTGGC ATCTGCAATG CCCATTCCTC GGATCGAGCC 
AAACAGCGCT CCATACAGTC GCTGATATCG TCGGGATCGG GATCGGGATC GGGATCGGGA 
TTTGAAGAAG GCTGGTGGCA ATCACCAACG CTGCAGGGTG GCCGGCAATT TGAGTATGTG 
ACCATACTAT TGGATCTGAA GCAGACTTTT CAAATATTTT CCGTGTGGCT AAAATCGGCC 
AACTCTCCGC GTCCAGCCTC ATGGATACTG GAAAAGTCCC TGGATGGGAT TAACTTTGAG 
CCATGGCAGT ATTTCGGATT GAGCGATGCA GATTGTCAGC GACGATGGAA TTTATCCGGG 
CAAAATGGCA AATACGTCTT CCAGAACGAT ACAGAAATCA TTTGCTCCAC TCAGTTTTCG 
AAGCCGGGTC CTTTGGAGAA CGGCGTCTTG CATGCTTCGC TGCTGAAGAA TCGCCCTGGA 
GCCACGGATC AAAGTCCGGA GCTAATGAAG TTCATAACGA CTCGGTACAT AAGGATTCGC 
CTGCAGGGCA TGCACTCCAC GGCCAATCAG GACAACAGTC TGGACTGGCT GCTGGACTCT 
CCGTCGCTGG AGAAGCACAG CTTCTACTCG CTGTCCCAGT TGAAGGTGAG TGCCCGTCTG 
GATTGTAATG GGCACGCCAA TCGCTCCCAC GAGTCGCCAG ATGACCCTCT GATGCAGTGC 
ATCTGCCAGC ACAATACCTG TGGTGCTCAG TGCGAACAGT GCTGTCCGCT TTTCCAGGAT 
CGCCCCTATC AAATGGGCGG AGAGTGCGAG ATATGCCAAT GCTATGGTCA CGCCGAGAGC 
TGCACCTATG ATCCCTTCCT GGACAAGGGC ATTTGCCAGA GCTGCTCGAA TAACACGGCT 
GGCATTGAGT GCGAGTTTTG CGAGATGGGA TTTTACAGGG AGCTGGATGC ACCGCTCACC 
GATCCCTGTT TGCCCTGCTC CTGTAATCCT GCTCGTTCCA CGGGTGGTTG CCAGTCGGAT 
GGAGGAAGCT GTAACTGCTT GGAGGGTTTC CAGGGCAAGA ACTGCGAGGA ATGTGCTCCG 
GGCTACTACG GAGACGATTG CAAACGGTGT GAGTGCGACG AAAGGGGAAG TCTTGGCTCC 
ACGGGTTCCT GCTCGGGTGT GTGCCAGTGC AAGCTCAATG TGGAGGGCAG CACCTGCTCC 
GAGTGTGCGC CCGGATATTT TGATCTAAGC GCAGAGAATG CAGAGGGATG CACCAGCTGT 
TGGTGCTCAG GTGTCTCGCA AACATGTCAC AGTGCCAAAC TGCAGACATT GGCCTTTGAA 
ACCCTCAACG ATTGGAAAAT AACCGATATT CAAAGAGTGA AACCAATTTC AATACCAGTT 
GATGCCGAAA CAAACCGTTT GATCTTTGCC AACGAACTGG ATGAAGTGGA GGCCATTTAC 
TGGCAGGCTT CGTTGGGTTA CTTGGGAAAT CGCTTGACCA GCTATGGGTC GAGACTGCAG 
CTGGTGTTGT CGTGGGATGT GATAAGAGGC GATAGATCGG GAAAGCCCAC GACGGGGCCC 
AATGTCATCC TAGTCGGCAA GAATGGACTG AAGATTGCCT TCGGCGATGA ATCCTTGGAC 
GGATTGGGAA TCAACTTGAA TGTAACCCTA ACAGAGGTTG GCTGGTATCA CGTGCCACCC 
ACTGTCGTTG ATATAAAAAC AAGACTTCGG CGAACCGAGG GCGGTGACTA TCACGGAGAA 
TCTGTTACCC GTTCCCAGTT TCTCTCCGTT CTCGTTTCCC TCGATGCTGT CCTTATTAGA 
GCGGCTTTTC ACACGGATCA AGGGGAAACA TCACTCGAGA GAGCAGTCAT CTATTCAGGA 
GGTGTGGAGT TGGGCGGAAA GTCTTCCAGC CAAGTGGAGC AATGCCTCTG CCCAGCTGGC 
TATACCGGTC TGTCCTGCGA GGGCTGCGCC TTTGGATTCA AGCGGATTTA CGAGAACACC 
TCGGACCATC AGATTCTGAG CAAGTGCATC CCGTGTCCCT GCAACGGACA CTCAAACTCC 
TGCGATCTGC AGAGCGGAAA TTGCGGCGAC TGCATGCACA ACACTTTTGG AGATCGTTGT 
GAGCGTTGCC AGTTGGGTTA CTACGGGAAT CCCCTGCAGG GAACCCCCAA TGACTGCAAG 
CGATGCGCCT GTCCCCTGTC CGAGGATTCC AACAACTTCT CGCCCAGTTG TCAGCTAAAG 
AGCTACAACT ACATGGACCT GAATCCGCAG TTCGAGCTGA TAGAGCATGC GGAGTACATA 
TGCACCCAAT GTCCGGAAGG TTATACGGGT GATCATTGTC AGGTGTGCGA CGATGGGTAC 
TTCGGGAATC CACGCCAGCC GGGAAGCAGT TGTCAGCGCT GCGATTGCGC CGGAGGGCCC 
TGCAACGTGA CCACCGGGGA GTGCATCACG TGCCGCGGAA ACACAGAGGG TTGGCACTGC 
GAGCGCTGCA AGCTGGGCTA CTGGGGTGAT CCGGCCGTGG GCTGTGATCC CTGTCATTGC 
CACACGGAAG GATCGGAGAG CGGACTATGC GACAGCACCG ATGGTCAATG CCTGTGTAAG 
CCACGTTATG CTGGACAGAA GTGCGATGAA TGTGACGTGG GCTATGCTAA TGTTGAGCTA 
CGCTGTCCAT CCTGCAACTG CGATCCATTG GGCTCGTTGG TCCAGGACAG ATGTGATCCC 
CACACTGGAC AATGCCATTG TAAGGAGGGC GTTATGGGTG CCAAATGCCA CGAGTGCCAG 
GATGGCTACT TCGGCATGAA TGCTGTGGCC TATCGAATGG ATGACCTTGC TGCACTGCGT 
CAAAACTCCG ATAGCGACGA TGACGAATGG GAATTGGTTC CAGATACTGA AGATCCCAAT 
AGCGAATCCA CGGTGGCCTG CGAGGAATGC CACTGCAGCA GTGTGGGATC CCTGTCATCG 
GATTGCGATA AGCGAACTGG GCAATGCGCC TGCCTGGCCA ACGTCACCGG GAGGAGATGC 
GACAAGTGTC GACCGGGTCA CTGGAATCTC ACGGCGGGCG AGGGCTGCCG GGATTGCCGA 
TGCGATCCTC ATGGTTCCCG AGGTCACGAG TGCAACCCGT GGACGGGCCA ATGCGACTGC 
AAGATCGGGG TTGGAGGTCA GCACTGTAAC GAGTGTACGG AGGGATTCTT TGGCTTCAGC 
ACGGAGGGTT GTCAGCGGTG CTCGGCCTGC AGGTCGGAGG GTCAGGTGTG TGACCCGCAC 
AATGGACGCT GCATTTGCCC CAAGTTCACC CGTGGCCTGG GATGTGGACA GTGTGTGCCG 
GGCACCTGGG GCTGGCAGGC TCGATTGGGT TGCCGGGAAT GCGAGTGCGA TCACATTGGC 
TCCATTGGTC AGCAGTGCAG CACCGGCGAT GGACAATGCC AGTGCCGAGA GGGATATTCG 
GGCAGAAAGT GCGACACTTG CGCCATCGGG TATTTCGGCT ATCCGGAGTG CCGACGATGC 
GGCTGCGATG CGGAGGGTTC CTTCACCCAG GCGGATGGCT CAATAGCCTG TGACTCGAAT 
GGCCAATGCC CGTGCAAATC CCTTGTGGTG GGTCTCAAAT GCGATACCTG CATGCAAAGC 
ACTTTTGGAT TGTCCGCACA AAATCCCGAA GGATGCACCC GATGCTTCTG CTTCGGGAGA 
TCCGGTGAGT GCGAGCAGAG CGACTTGTCT TGGGGACACA TCCGTATGGC GGAGTCCAGA 
AATCTCAGCG TTCAGCAAAT AAGACCGCAC TCGGTACCCA GCTCAGACTA CGAGTACATA 
GTGGTGGTTC AGATGGAGGG CAGCAGTTTC CATCGCGAAG ACGCGGAGAT CCAGCGCATG 
AATGATCTGA GCTTAGTGCC CAAATCTACG GGGAATGTTT CCATCGGAGC ATATGGACAA 
TTTTACCATC CGCTCTACTT CCAACTGCCT CCGCAGTTCT ACGGAGATCG GACTAGTAGC 
TATGGTGGCT TTTTGTACTT CACCCTGATC ACCGAAGGTG CCCACAAGCC CTTGGAGCGA 
AATATTTTGG GTCAGTATCC ATTGGTTCAG TTACACGCGC ACTCCAAGTT GCTACTGGAC 
TTTTACGAGT ACGAAGAGTT CGAGTACTCC TTGAACGTGA CCCATCGGGT GCCGCTGCAT 
GAGTCCTTCT GGAAGTACCA CCACACCAGC CAGGCAGTGG ATCGTAATAC TCTAATGGCT 
GCCCTCCAAA ACATTCGACA CATCTTCATT CGAGCCTTTG CTTTTGCCGA TTTCCAGGAA 
GTTGTCCTTC AAAATGTACA CATGGATGCG GCCATCTATA TCAAGGGCTC CACCAATCTT 
ATAGCCAAGG GAGTCGAACG GTGTAAGTGT CCCAAGCGGT TTGATGGACT TTCATGCCAG 
GATCCGGGAA GATCCTTCTA CCGTTGGCGG AATACCACTA TTGTGGAGAG TGTATTCATT 
GAGGATCTGA TAGGAAGAGC AGCTCCCTGT CATTGTAATG GTCGTAGCAG CGATTGTGAT 
CGCGAAACTG GAGTCTGTCA GAACTGTCGA GGTAACACTG GAGGAGATCA CTGCCATCAG 
TGTGCTGAGG GATTCTACGG CGATCCCAAT TCTCCACACG GTTGTCAGGC TTGTCCGTGC 
CCGGAGACCA ATAGGAACTT TGCCCGGGGC TGCAATGTCT GGGACGGAGA AGTCAGTTGT 
GTCTGCAAGC CGGGTTACAC GGGCAGATTG TGTGAGCGGT GCCAGGCGGG ATATTTTGGA 
GATCCTATGA GATATCCAAA CACTACTTGC CAACCTTGCA ACTGTCACCC AGATGGAATC 
CAAACCGAAG GCTGCGATGT AGAGACTGGT AGATGCTACT GTCGAGAAGG AGTTACTGGG 
CTAAAGTGCG ACAAGTGTCA GGCGGAACGT CATCATCTGG TGGACAACGG ATGCAAAATC 
TGTGACAATT GCACTCTTCT GCTTCTGGAT TATATGGAGC TGGTGGGCAA CAAGCTTCGT 
CGTGGAATGC ACAACATGGA TTTGACTGGA ATTCCGGCGC CATATCGAAA ATTGTCAGAG 
TACGAAAGCG CCTATGAGAA ATGGAATGGG CGCCACTGGG ACTTTAGCCA GACCAAACGG 
CGTCTACAGG ATTACGACTC CGCTGACATC CTAAAGCTGG AGGCCCACGC CGAAAATTTG 
AAGTTTCAAT CCCGCAAAGC AGTGGCCACC ATTGGAAAAC GGGAGTTTGC CATAAAATCG 
ATGCGGGAAG ATGCTGTAAC CCAACAGCAT AGTGTCGGCT TACTCCGCTC GGAGATTCTT 
CAAACCCTTT CCGATTTACA TGGCTATGGA AAGAGTGCCC ATTATCTCAG TCTGCCTACT 
GCTCTCAAGC AGGCCCGATT CTACCTGCAG GCCATCCGGG AGCACGACCA AATGGTTCAG 
GGAATCCGCA GTACCAACGA CTGTGCATGG AAGCATTTTT ATGCCATGGG CAACGCATCG 
GATGCGTCGT TCGACGAGAG CGGCCGCTTG GAGATGCTCT GGCGGGACTT GAATCAGACC 
AATCATCGAG TGGTAGACAT GCGGCTGCAG GTGGATCGCG TGCAAGAGGT GGAGAACGAA 
GCGGAGGACG TTCTGGAACA TGTGCGCAAT TTGAGTATTC GCGTGGGTGA ATCCCACCAG 
GAGCTGGATG AACTTAACCA GCGTATTTCC GATCACCTAG ATCCGGGCTA CCTAGAACAG 
GGCGAGGGCC TGCTAAGGCT GACAGTCCAG CGGCAGATAA TGCTCAATGG GCATCTTAAC 
CAATTGGATG GCTACCGTAT ACTACTAAAT ACCACATTGG GCGTAAAGAC TGAGCAGCAG 
CGGGAGGTGC GTAAACACTG GCTTCCCAAG GCGGAAAAGC ACGCCAGTCA TCTGCTGGCG 
CGATCCAATG AGTACGCCCG CAAGTTTCAA CCCACCAGAA ATGGAGCCCG CATAGCCATG 
CTGGCCAGTT CGGCCCACAG CAATATCACT GAGGCCATCA ACGATGCCCG GCTAGCATCA 
ATCCTGGCCA AGGAACGGGT GTACGAGGCG CAGAGAACAC TCTATCCCAG TGACGGCAGC 
TCCATGATCG AGCGAGCCAA GCACTCGCTG CACAGATCAA AGCAACTTCA GCAAGAAGCG 
CTCAAGCAGA TGCACAAATC CAATGTGTTG AAGGACAAAC TGCATCGCCA GGAGCAGCAG 
GTGGAGGGCA TCAAGTCCAC GATTTACGAT TCCGGACTGC GCACCAACAA CATATCCGGA 
CAGCTGCAGG GTCTTTCAGA TGGTTCAGCA CGTCGTCAAG CGAAGGATAG TCTGGAAATG 
GCTGATAGAA CGGGAGAACA AATGCGTGCT GAACTGCAAA AGGCGAAGGA TATGCAGAAG 
TCCATCCAAA ACATGCGAAA TTCGTTCTCC AATCTGGAAC CCGACTGGGA AATCAAGCTG 
GGAATGGCCC AAGAGAACAT ATCCCTGACC CAGACTAACT TGCGTCTGGC CAATGTCTCA 
TTGAGCTACC TAGAGCAGCA GGCCGAGAAG GAGCAACAGG TGTTCGAGGT GTGGAACAAC 
AGCATGGCCC AGCAACTGCA GCAGCTCCGG GATCAAATAG CCAAGGCCAG ACATGCAGCA 
GAAGCGATCG ATGTTTCCCT GGAATCGCTG GGTCCTAAAT GCATTCGATC CTATTTGCCC 
GCCTCCTACG GTTTGAGTAC CTCGAATAAG ATTCGTATGA GCTTCGCCTT ATCCAATCAC 
CTCGAGAGCT CTCCATTGAT TCACCTGGCC AGCAGCGAGG GGCGTCACAT CACCCTGGAG 
CTGTACAAAC GTCGTGTGCG CCTGGTGTGG AATCTTGGTG GCACCACCGC AACCGTTACG 
CATCCCATGG TGGTGCATAC TCGTGACCCC AAGTACGACG ATGCCTGGTA CCATGTGGAG 
GCCAATAGGA CACTTAATCT GGGCAGCCTG GTCGTGCGTC GAATGAACAA CTATGGCGAG 
CTGACTCCGC CTAACCCGGT CATCATAACA GGATCCACGG ATACGGAGCA CACACGTTTC 
TATCAGTCTC GAAGCGATCG AATTTCGCTG GGAGGCTTTG CATCGAAAGA TTTGCAATTC 
ACGCCGGGAC TCAATGTGGT GGTGCATCAG GTGGAGGTGG ACAACAAACC GCTGGGTCTG 
TGGAACTTCG TGACCAGCGA GGGCAGCTGC GGTGGATCAA TGGTTGGAGC TAAGGAGTCT 
TCGGCCTCGT CCACGGCTCG CCATTTCAAT GGATTAGGCT ATGCCCAGTT GATGAAGACC 
CGACCACGAC CCACTCGCAA AAACCTGTTC TCCGTGCAGA TGACTTTCCG CACGTTGGAC 
GAAAATGCCT TGCTCTTCTT GGCGGTTGAT GACAAGAACA ACCGCTCGGT ATCGGTAACT 
TTGAGCCGGG GTCGGATCAT GTTCCGCATT GACTATGGCG ATGAGTCCAA GCTGGAAATC 
AACACCACTA AGAAGTATAA TGTGGGCCAG TGGATCAAGA TCGAGGCTGC GCGAGAGTTC 
TCGGCGAAAA GAAGTACTGA AAATGGAATG CTCCGAGTAA ACAACGATCG TCCAATTTCC 
GGAGCACCTA CACTACCCGT AAACATTCAC TTGCTTCCGG ATCTTTCCAA GACTGTCTAT 
TATCTGGGCG GTGTGCCACC AGGTTTCACT TCGGGAACCT CAAAGGCGCC TGGTGCTGAT 
AATCCCTTCT TGGGATGTAT GAAGGATGTC CAGGTAAATG GCGAGACCTA CGATCCTCTG 
GAGAGCTCAA GCTACTACGG TGTGGAGCCT TCCTGCAAGA ACATGATTAC CAAGGCTGGT 
TTCTCGGGCA ATGGTTACTT GGAGCTGCCA TCGCAGTCAC TACGAAAGCG CTCCAATACC 
GCTTTGGTGT TCCGCACCCT TCAGCCCGAT TGCCTGCTGC TCCTTGCTGC CTATCCGCCT 
GAGATCCTGG GCGACTATGA TGCTAAGGAC ATTAAGGGCA ACTTCTCCAT TAGCCTGGTC 
GATGGTCAAT TGCATGTTTG GGTTAACTCT GGTCGCAGCT TCATCAAGAT GTCCTCGAAC 
TCCAGTCAGA TGAACGATGG CGAGTTTCAC GTGGTACATT TGATCAAGAC CGGTCGCAAG 
CTGGAGCTGA TGGTGGACGA TGAGCTGCAA GAGATTCGCA ATCTAAACGG CAATCCCACG 
GTGGTCAGCC TGCCGAGGGA TGCAGGTGGC CTGTATATAG GTGGAGCTCC GCCGCACGAA 
AGTTATACGC CATTGGCGCC GACTTTTGTA AATCTAGAAG GAGCCATTCG GGATGTGGTG 
TTCAACAATC GGACCATTAA CTTTAACGAT GCCCTGACCT TTGCCAACGT CCAGATTGGA 
CGTAATGGTC CACTGATGGG CAGTCTGAAA GGAGGACTGT ACGATGTCCT CCTGAAAACC 
GAACCCATGA TTGGAAAGAG TTTTACGGCC TCGCCTGAGG GCTGCAAGCG GATCGGCAGC 
TATTCCTACG AGCCGAATGC ATTCAAGTTT GGAGACGATA TCTATAGCTA TTCGCAGCTG 
AAGCTACCGG AGCGTCATTT CTGGCAGCGC AACTTCCATC TGAGCTTCGA TTTCCGCTCC 
TTCTATCCCA ATGGAATGCT CTACTTGTCG CCGGGAAGCA AGGAGAAACC CAAGCACTAC 
GTGGCTCTCG TCCTCAAGGA CGGACAGCTG GTCCTGGTGG TGCGAGGTCG TCGGCGCGAG 
GAACTCCAGT TGACCGCCAA GTTAAACGAT GGCGAATGGC ACCGGGTGAC GATTAGCTGT 
CACGATCGCA AGGTCACCAT GTCGGTGGAA ATAGGACGTA CAGATCAAAA GACTTCCGCC 
CAGATGAAGC TGCCCAAGAA GATTGGCGCC TCTCAGCTTT TGCTGGTTGG TGGGCTACCA 
CAGTCTCCAG TCAAGGTCTC CTCCGATCTC TATGTCCGTC TGGAACCATT CAAGGGTTGT 
CTGCGCCGGG TGAGCATCAA CAACAACACC CAGGATCTGG CCCGACCCGG CAAACATTCG 
AATGTGGGTC AGTGCTTTCC CACGGTGGAG AGAGGCAGTT ACTTTCCCGG CGATGCCTAT 
GCCATTTACA AGAAAAACTT CAATGTGGGC AAGTATCTCG ATCTGGAAAC GGAGTTTAGG 
ACCTCGGAGC TATCGGGCAT TCTGCTGAGT GTCTCTGATC CAAATGGATT CCCAGCGCTT 
TCACTGGAAC TGCATAATGG CAATATAATA TTCTCTTGCG ATCCTGGAAA CGGAGCGCCC 
ATGCGTGTGG AGTCATCACT GCCCACCAAA TATGCCCTAT GTGACAACAA ATGGCATAAT 
ATTTCAGCTC TCTACGATGG CGAACAGATT GTCCTGCGCA TCGATCAATT GCCAGCCGTG 
ATCAGTGTGG GTAATCAGGG GAATGCTGGA AAGGTGCAAA CCCGATCCCC GCTCTATATT 
GGCGGTTTGC CAGAGAGTGC TCCCAGTGGT TCACTGATAT CGCGGGAGAA CTTCAAGGGC 
TGCATCCGGC ACGTGTCCAT ACGGAACGAG AGACGCGACT GGATCGAGAT GGAGGACCTG 
AGGAACGTGC TGCTCAGCGA GTGCCTCGTG TCCAGCGATG AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wb ( NM_057442.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476790.3 Drosophila melanogaster wing blister (wb), transcript variant G, mRNA.

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CloneID	OFa15183
Clone ID Related Accession (Same CDS sequence)	NM_057442.5
Accession Version	NM_057442.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wing blister, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057442.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAATCG GAAATTTTAT CAAATGGCCA CTGGGCCTGA TCCTCATCCT GCTGGTCACC 
CACTGTCAGG CCAAAAGGCA GCGGAAACGG TTGCGTGGAT TCTCCACTGC CAAGGACCTA 
CAGGATTATA GCATGCCGCT CAACGACAAC TCCACGGAAC TCCAGCAGAA CATTGTGTCC 
TGGACAGGAG CTCGAATTGC GTCGAGTCCC GATAAGAAGA AGAAGTCACC GCAATCATCG 
AGACGTCACA AAACCAAAAG TGGCCTGATC ATTGAAAGTA ACATTGCCGA CTCGGAATCG 
ATGTTGGCTA TTGCCTCCTC GGAATCCGTC ACCAAGAAGA AGCCGCCGCA GCGTCGTCGC 
AACGGAAAAT TACAGAAATC CGGAGGAGGA GCAGGAGGTG GAGCCCTTCG ATTGCTCAAA 
ACGGAGGACG TGTACTCATC CAGTTTCTCC GGCGGCCTCT ATCCGCCGCT CTTCAATGTG 
GTTCCACGGG CTCAGATCTC GGTGAATGCC ACTTGTGGCC AGAACGGTGC CGAGGAGTAC 
TGCAAACAGG TCGGCGCCAA GCCGTGTGGC ATCTGCAATG CCCATTCCTC GGATCGAGCC 
AAACAGCGCT CCATACAGTC GCTGATATCG TCGGGATCGG GATCGGGATC GGGATCGGGA 
TTTGAAGAAG GCTGGTGGCA ATCACCAACG CTGCAGGGTG GCCGGCAATT TGAGTATGTG 
ACCATACTAT TGGATCTGAA GCAGACTTTT CAAATATTTT CCGTGTGGCT AAAATCGGCC 
AACTCTCCGC GTCCAGCCTC ATGGATACTG GAAAAGTCCC TGGATGGGAT TAACTTTGAG 
CCATGGCAGT ATTTCGGATT GAGCGATGCA GATTGTCAGC GACGATGGAA TTTATCCGGG 
CAAAATGGCA AATACGTCTT CCAGAACGAT ACAGAAATCA TTTGCTCCAC TCAGTTTTCG 
AAGCCGGGTC CTTTGGAGAA CGGCGTCTTG CATGCTTCGC TGCTGAAGAA TCGCCCTGGA 
GCCACGGATC AAAGTCCGGA GCTAATGAAG TTCATAACGA CTCGGTACAT AAGGATTCGC 
CTGCAGGGCA TGCACTCCAC GGCCAATCAG GACAACAGTC TGGACTGGCT GCTGGACTCT 
CCGTCGCTGG AGAAGCACAG CTTCTACTCG CTGTCCCAGT TGAAGGTGAG TGCCCGTCTG 
GATTGTAATG GGCACGCCAA TCGCTCCCAC GAGTCGCCAG ATGACCCTCT GATGCAGTGC 
ATCTGCCAGC ACAATACCTG TGGTGCTCAG TGCGAACAGT GCTGTCCGCT TTTCCAGGAT 
CGCCCCTATC AAATGGGCGG AGAGTGCGAG ATATGCCAAT GCTATGGTCA CGCCGAGAGC 
TGCACCTATG ATCCCTTCCT GGACAAGGGC ATTTGCCAGA GCTGCTCGAA TAACACGGCT 
GGCATTGAGT GCGAGTTTTG CGAGATGGGA TTTTACAGGG AGCTGGATGC ACCGCTCACC 
GATCCCTGTT TGCCCTGCTC CTGTAATCCT GCTCGTTCCA CGGGTGGTTG CCAGTCGGAT 
GGAGGAAGCT GTAACTGCTT GGAGGGTTTC CAGGGCAAGA ACTGCGAGGA ATGTGCTCCG 
GGCTACTACG GAGACGATTG CAAACGGTGT GAGTGCGACG AAAGGGGAAG TCTTGGCTCC 
ACGGGTTCCT GCTCGGGTGT GTGCCAGTGC AAGCTCAATG TGGAGGGCAG CACCTGCTCC 
GAGTGTGCGC CCGGATATTT TGATCTAAGC GCAGAGAATG CAGAGGGATG CACCAGCTGT 
TGGTGCTCAG GTGTCTCGCA AACATGTCAC AGTGCCAAAC TGCAGACATT GGCCTTTGAA 
ACCCTCAACG ATTGGAAAAT AACCGATATT CAAAGAGTGA AACCAATTTC AATACCAGTT 
GATGCCGAAA CAAACCGTTT GATCTTTGCC AACGAACTGG ATGAAGTGGA GGCCATTTAC 
TGGCAGGCTT CGTTGGGTTA CTTGGGAAAT CGCTTGACCA GCTATGGGTC GAGACTGCAG 
CTGGTGTTGT CGTGGGATGT GATAAGAGGC GATAGATCGG GAAAGCCCAC GACGGGGCCC 
AATGTCATCC TAGTCGGCAA GAATGGACTG AAGATTGCCT TCGGCGATGA ATCCTTGGAC 
GGATTGGGAA TCAACTTGAA TGTAACCCTA ACAGAGGTTG GCTGGTATCA CGTGCCACCC 
ACTGTCGTTG ATATAAAAAC AAGACTTCGG CGAACCGAGG GCGGTGACTA TCACGGAGAA 
TCTGTTACCC GTTCCCAGTT TCTCTCCGTT CTCGTTTCCC TCGATGCTGT CCTTATTAGA 
GCGGCTTTTC ACACGGATCA AGGGGAAACA TCACTCGAGA GAGCAGTCAT CTATTCAGGA 
GGTGTGGAGT TGGGCGGAAA GTCTTCCAGC CAAGTGGAGC AATGCCTCTG CCCAGCTGGC 
TATACCGGTC TGTCCTGCGA GGGCTGCGCC TTTGGATTCA AGCGGATTTA CGAGAACACC 
TCGGACCATC AGATTCTGAG CAAGTGCATC CCGTGTCCCT GCAACGGACA CTCAAACTCC 
TGCGATCTGC AGAGCGGAAA TTGCGGCGAC TGCATGCACA ACACTTTTGG AGATCGTTGT 
GAGCGTTGCC AGTTGGGTTA CTACGGGAAT CCCCTGCAGG GAACCCCCAA TGACTGCAAG 
CGATGCGCCT GTCCCCTGTC CGAGGATTCC AACAACTTCT CGCCCAGTTG TCAGCTAAAG 
AGCTACAACT ACATGGACCT GAATCCGCAG TTCGAGCTGA TAGAGCATGC GGAGTACATA 
TGCACCCAAT GTCCGGAAGG TTATACGGGT GATCATTGTC AGGTGTGCGA CGATGGGTAC 
TTCGGGAATC CACGCCAGCC GGGAAGCAGT TGTCAGCGCT GCGATTGCGC CGGAGGGCCC 
TGCAACGTGA CCACCGGGGA GTGCATCACG TGCCGCGGAA ACACAGAGGG TTGGCACTGC 
GAGCGCTGCA AGCTGGGCTA CTGGGGTGAT CCGGCCGTGG GCTGTGATCC CTGTCATTGC 
CACACGGAAG GATCGGAGAG CGGACTATGC GACAGCACCG ATGGTCAATG CCTGTGTAAG 
CCACGTTATG CTGGACAGAA GTGCGATGAA TGTGACGTGG GCTATGCTAA TGTTGAGCTA 
CGCTGTCCAT CCTGCAACTG CGATCCATTG GGCTCGTTGG TCCAGGACAG ATGTGATCCC 
CACACTGGAC AATGCCATTG TAAGGAGGGC GTTATGGGTG CCAAATGCCA CGAGTGCCAG 
GATGGCTACT TCGGCATGAA TGCTGTGGCC TATCGAATGG ATGACCTTGC TGCACTGCGT 
CAAAACTCCG ATAGCGACGA TGACGAATGG GAATTGGTTC CAGATACTGA AGATCCCAAT 
AGCGAATCCA CGGTGGCCTG CGAGGAATGC CACTGCAGCA GTGTGGGATC CCTGTCATCG 
GATTGCGATA AGCGAACTGG GCAATGCGCC TGCCTGGCCA ACGTCACCGG GAGGAGATGC 
GACAAGTGTC GACCGGGTCA CTGGAATCTC ACGGCGGGCG AGGGCTGCCG GGATTGCCGA 
TGCGATCCTC ATGGTTCCCG AGGTCACGAG TGCAACCCGT GGACGGGCCA ATGCGACTGC 
AAGATCGGGG TTGGAGGTCA GCACTGTAAC GAGTGTACGG AGGGATTCTT TGGCTTCAGC 
ACGGAGGGTT GTCAGCGGTG CTCGGCCTGC AGGTCGGAGG GTCAGGTGTG TGACCCGCAC 
AATGGACGCT GCATTTGCCC CAAGTTCACC CGTGGCCTGG GATGTGGACA GTGTGTGCCG 
GGCACCTGGG GCTGGCAGGC TCGATTGGGT TGCCGGGAAT GCGAGTGCGA TCACATTGGC 
TCCATTGGTC AGCAGTGCAG CACCGGCGAT GGACAATGCC AGTGCCGAGA GGGATATTCG 
GGCAGAAAGT GCGACACTTG CGCCATCGGG TATTTCGGCT ATCCGGAGTG CCGACGATGC 
GGCTGCGATG CGGAGGGTTC CTTCACCCAG GCGGATGGCT CAATAGCCTG TGACTCGAAT 
GGCCAATGCC CGTGCAAATC CCTTGTGGTG GGTCTCAAAT GCGATACCTG CATGCAAAGC 
ACTTTTGGAT TGTCCGCACA AAATCCCGAA GGATGCACCC GATGCTTCTG CTTCGGGAGA 
TCCGGTGAGT GCGAGCAGAG CGACTTGTCT TGGGGACACA TCCGTATGGC GGAGTCCAGA 
AATCTCAGCG TTCAGCAAAT AAGACCGCAC TCGGTACCCA GCTCAGACTA CGAGTACATA 
GTGGTGGTTC AGATGGAGGG CAGCAGTTTC CATCGCGAAG ACGCGGAGAT CCAGCGCATG 
AATGATCTGA GCTTAGTGCC CAAATCTACG GGGAATGTTT CCATCGGAGC ATATGGACAA 
TTTTACCATC CGCTCTACTT CCAACTGCCT CCGCAGTTCT ACGGAGATCG GACTAGTAGC 
TATGGTGGCT TTTTGTACTT CACCCTGATC ACCGAAGGTG CCCACAAGCC CTTGGAGCGA 
AATATTTTGG GTCAGTATCC ATTGGTTCAG TTACACGCGC ACTCCAAGTT GCTACTGGAC 
TTTTACGAGT ACGAAGAGTT CGAGTACTCC TTGAACGTGA CCCATCGGGT GCCGCTGCAT 
GAGTCCTTCT GGAAGTACCA CCACACCAGC CAGGCAGTGG ATCGTAATAC TCTAATGGCT 
GCCCTCCAAA ACATTCGACA CATCTTCATT CGAGCCTTTG CTTTTGCCGA TTTCCAGGAA 
GTTGTCCTTC AAAATGTACA CATGGATGCG GCCATCTATA TCAAGGGCTC CACCAATCTT 
ATAGCCAAGG GAGTCGAACG GTGTAAGTGT CCCAAGCGGT TTGATGGACT TTCATGCCAG 
GATCCGGGAA GATCCTTCTA CCGTTGGCGG AATACCACTA TTGTGGAGAG TGTATTCATT 
GAGGATCTGA TAGGAAGAGC AGCTCCCTGT CATTGTAATG GTCGTAGCAG CGATTGTGAT 
CGCGAAACTG GAGTCTGTCA GAACTGTCGA GGTAACACTG GAGGAGATCA CTGCCATCAG 
TGTGCTGAGG GATTCTACGG CGATCCCAAT TCTCCACACG GTTGTCAGGC TTGTCCGTGC 
CCGGAGACCA ATAGGAACTT TGCCCGGGGC TGCAATGTCT GGGACGGAGA AGTCAGTTGT 
GTCTGCAAGC CGGGTTACAC GGGCAGATTG TGTGAGCGGT GCCAGGCGGG ATATTTTGGA 
GATCCTATGA GATATCCAAA CACTACTTGC CAACCTTGCA ACTGTCACCC AGATGGAATC 
CAAACCGAAG GCTGCGATGT AGAGACTGGT AGATGCTACT GTCGAGAAGG AGTTACTGGG 
CTAAAGTGCG ACAAGTGTCA GGCGGAACGT CATCATCTGG TGGACAACGG ATGCAAAATC 
TGTGACAATT GCACTCTTCT GCTTCTGGAT TATATGGAGC TGGTGGGCAA CAAGCTTCGT 
CGTGGAATGC ACAACATGGA TTTGACTGGA ATTCCGGCGC CATATCGAAA ATTGTCAGAG 
TACGAAAGCG CCTATGAGAA ATGGAATGGG CGCCACTGGG ACTTTAGCCA GACCAAACGG 
CGTCTACAGG ATTACGACTC CGCTGACATC CTAAAGCTGG AGGCCCACGC CGAAAATTTG 
AAGTTTCAAT CCCGCAAAGC AGTGGCCACC ATTGGAAAAC GGGAGTTTGC CATAAAATCG 
ATGCGGGAAG ATGCTGTAAC CCAACAGCAT AGTGTCGGCT TACTCCGCTC GGAGATTCTT 
CAAACCCTTT CCGATTTACA TGGCTATGGA AAGAGTGCCC ATTATCTCAG TCTGCCTACT 
GCTCTCAAGC AGGCCCGATT CTACCTGCAG GCCATCCGGG AGCACGACCA AATGGTTCAG 
GGAATCCGCA GTACCAACGA CTGTGCATGG AAGCATTTTT ATGCCATGGG CAACGCATCG 
GATGCGTCGT TCGACGAGAG CGGCCGCTTG GAGATGCTCT GGCGGGACTT GAATCAGACC 
AATCATCGAG TGGTAGACAT GCGGCTGCAG GTGGATCGCG TGCAAGAGGT GGAGAACGAA 
GCGGAGGACG TTCTGGAACA TGTGCGCAAT TTGAGTATTC GCGTGGGTGA ATCCCACCAG 
GAGCTGGATG AACTTAACCA GCGTATTTCC GATCACCTAG ATCCGGGCTA CCTAGAACAG 
GGCGAGGGCC TGCTAAGGCT GACAGTCCAG CGGCAGATAA TGCTCAATGG GCATCTTAAC 
CAATTGGATG GCTACCGTAT ACTACTAAAT ACCACATTGG GCGTAAAGAC TGAGCAGCAG 
CGGGAGGTGC GTAAACACTG GCTTCCCAAG GCGGAAAAGC ACGCCAGTCA TCTGCTGGCG 
CGATCCAATG AGTACGCCCG CAAGTTTCAA CCCACCAGAA ATGGAGCCCG CATAGCCATG 
CTGGCCAGTT CGGCCCACAG CAATATCACT GAGGCCATCA ACGATGCCCG GCTAGCATCA 
ATCCTGGCCA AGGAACGGGT GTACGAGGCG CAGAGAACAC TCTATCCCAG TGACGGCAGC 
TCCATGATCG AGCGAGCCAA GCACTCGCTG CACAGATCAA AGCAACTTCA GCAAGAAGCG 
CTCAAGCAGA TGCACAAATC CAATGTGTTG AAGGACAAAC TGCATCGCCA GGAGCAGCAG 
GTGGAGGGCA TCAAGTCCAC GATTTACGAT TCCGGACTGC GCACCAACAA CATATCCGGA 
CAGCTGCAGG GTCTTTCAGA TGGTTCAGCA CGTCGTCAAG CGAAGGATAG TCTGGAAATG 
GCTGATAGAA CGGGAGAACA AATGCGTGCT GAACTGCAAA AGGCGAAGGA TATGCAGAAG 
TCCATCCAAA ACATGCGAAA TTCGTTCTCC AATCTGGAAC CCGACTGGGA AATCAAGCTG 
GGAATGGCCC AAGAGAACAT ATCCCTGACC CAGACTAACT TGCGTCTGGC CAATGTCTCA 
TTGAGCTACC TAGAGCAGCA GGCCGAGAAG GAGCAACAGG TGTTCGAGGT GTGGAACAAC 
AGCATGGCCC AGCAACTGCA GCAGCTCCGG GATCAAATAG CCAAGGCCAG ACATGCAGCA 
GAAGCGATCG ATGTTTCCCT GGAATCGCTG GGTCCTAAAT GCATTCGATC CTATTTGCCC 
GCCTCCTACG GTTTGAGTAC CTCGAATAAG ATTCGTATGA GCTTCGCCTT ATCCAATCAC 
CTCGAGAGCT CTCCATTGAT TCACCTGGCC AGCAGCGAGG GGCGTCACAT CACCCTGGAG 
CTGTACAAAC GTCGTGTGCG CCTGGTGTGG AATCTTGGTG GCACCACCGC AACCGTTACG 
CATCCCATGG TGGTGCATAC TCGTGACCCC AAGTACGACG ATGCCTGGTA CCATGTGGAG 
GCCAATAGGA CACTTAATCT GGGCAGCCTG GTCGTGCGTC GAATGAACAA CTATGGCGAG 
CTGACTCCGC CTAACCCGGT CATCATAACA GGATCCACGG ATACGGAGCA CACACGTTTC 
TATCAGTCTC GAAGCGATCG AATTTCGCTG GGAGGCTTTG CATCGAAAGA TTTGCAATTC 
ACGCCGGGAC TCAATGTGGT GGTGCATCAG GTGGAGGTGG ACAACAAACC GCTGGGTCTG 
TGGAACTTCG TGACCAGCGA GGGCAGCTGC GGTGGATCAA TGGTTGGAGC TAAGGAGTCT 
TCGGCCTCGT CCACGGCTCG CCATTTCAAT GGATTAGGCT ATGCCCAGTT GATGAAGACC 
CGACCACGAC CCACTCGCAA AAACCTGTTC TCCGTGCAGA TGACTTTCCG CACGTTGGAC 
GAAAATGCCT TGCTCTTCTT GGCGGTTGAT GACAAGAACA ACCGCTCGGT ATCGGTAACT 
TTGAGCCGGG GTCGGATCAT GTTCCGCATT GACTATGGCG ATGAGTCCAA GCTGGAAATC 
AACACCACTA AGAAGTATAA TGTGGGCCAG TGGATCAAGA TCGAGGCTGC GCGAGAGTTC 
TCGGCGAAAA GAAGTACTGA AAATGGAATG CTCCGAGTAA ACAACGATCG TCCAATTTCC 
GGAGCACCTA CACTACCCGT AAACATTCAC TTGCTTCCGG ATCTTTCCAA GACTGTCTAT 
TATCTGGGCG GTGTGCCACC AGGTTTCACT TCGGGAACCT CAAAGGCGCC TGGTGCTGAT 
AATCCCTTCT TGGGATGTAT GAAGGATGTC CAGGTAAATG GCGAGACCTA CGATCCTCTG 
GAGAGCTCAA GCTACTACGG TGTGGAGCCT TCCTGCAAGA ACATGATTAC CAAGGCTGGT 
TTCTCGGGCA ATGGTTACTT GGAGCTGCCA TCGCAGTCAC TACGAAAGCG CTCCAATACC 
GCTTTGGTGT TCCGCACCCT TCAGCCCGAT TGCCTGCTGC TCCTTGCTGC CTATCCGCCT 
GAGATCCTGG GCGACTATGA TGCTAAGGAC ATTAAGGGCA ACTTCTCCAT TAGCCTGGTC 
GATGGTCAAT TGCATGTTTG GGTTAACTCT GGTCGCAGCT TCATCAAGAT GTCCTCGAAC 
TCCAGTCAGA TGAACGATGG CGAGTTTCAC GTGGTACATT TGATCAAGAC CGGTCGCAAG 
CTGGAGCTGA TGGTGGACGA TGAGCTGCAA GAGATTCGCA ATCTAAACGG CAATCCCACG 
GTGGTCAGCC TGCCGAGGGA TGCAGGTGGC CTGTATATAG GTGGAGCTCC GCCGCACGAA 
AGTTATACGC CATTGGCGCC GACTTTTGTA AATCTAGAAG GAGCCATTCG GGATGTGGTG 
TTCAACAATC GGACCATTAA CTTTAACGAT GCCCTGACCT TTGCCAACGT CCAGATTGGA 
CGTAATGGTC CACTGATGGG CAGTCTGAAA GGAGGACTGT ACGATGTCCT CCTGAAAACC 
GAACCCATGA TTGGAAAGAG TTTTACGGCC TCGCCTGAGG GCTGCAAGCG GGATTTTAGT 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476790.3
CDS	695..9700
Translation	MQIGNFIKWPLGLILILLVTHCQAKRQRKRLRGFSTAKDLQDYSMPLNDNSTELQQNIVSWTGARIASSPDKKKKSPQSSRRHKTKSGLIIESNIADSESMLAIASSESVTKKKPPQRRRNGKLQKSGGGAGGGALRLLKTEDVYSSSFSGGLYPPLFNVVPRAQISVNATCGQNGAEEYCKQVGAKPCGICNAHSSDRAKQRSIQSLISSGSGSGSGSGFEEGWWQSPTLQGGRQFEYVTILLDLKQTFQIFSVWLKSANSPRPASWILEKSLDGINFEPWQYFGLSDADCQRRWNLSGQNGKYVFQNDTEIICSTQFSKPGPLENGVLHASLLKNRPGATDQSPELMKFITTRYIRIRLQGMHSTANQDNSLDWLLDSPSLEKHSFYSLSQLKVSARLDCNGHANRSHESPDDPLMQCICQHNTCGAQCEQCCPLFQDRPYQMGGECEICQCYGHAESCTYDPFLDKGICQSCSNNTAGIECEFCEMGFYRELDAPLTDPCLPCSCNPARSTGGCQSDGGSCNCLEGFQGKNCEECAPGYYGDDCKRCECDERGSLGSTGSCSGVCQCKLNVEGSTCSECAPGYFDLSAENAEGCTSCWCSGVSQTCHSAKLQTLAFETLNDWKITDIQRVKPISIPVDAETNRLIFANELDEVEAIYWQASLGYLGNRLTSYGSRLQLVLSWDVIRGDRSGKPTTGPNVILVGKNGLKIAFGDESLDGLGINLNVTLTEVGWYHVPPTVVDIKTRLRRTEGGDYHGESVTRSQFLSVLVSLDAVLIRAAFHTDQGETSLERAVIYSGGVELGGKSSSQVEQCLCPAGYTGLSCEGCAFGFKRIYENTSDHQILSKCIPCPCNGHSNSCDLQSGNCGDCMHNTFGDRCERCQLGYYGNPLQGTPNDCKRCACPLSEDSNNFSPSCQLKSYNYMDLNPQFELIEHAEYICTQCPEGYTGDHCQVCDDGYFGNPRQPGSSCQRCDCAGGPCNVTTGECITCRGNTEGWHCERCKLGYWGDPAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELRCPSCNCDPLGSLVQDRCDPHTGQCHCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPNSESTVACEECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQARFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQTNHRVVDMRLQVDRVQEVENEAEDVLEHVRNLSIRVGESHQELDELNQRISDHLDPGYLEQGEGLLRLTVQRQIMLNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEKHASHLLARSNEYARKFQPTRNGARIAMLASSAHSNITEAINDARLASILAKERVYEAQRTLYPSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISGQLQGLSDGSARRQAKDSLEMADRTGEQMRAELQKAKDMQKSIQNMRNSFSNLEPDWEIKLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEVWNNSMAQQLQQLRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHLASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAWYHVEANRTLNLGSLVVRRMNNYGELTPPNPVIITGSTDTEHTRFYQSRSDRISLGGFASKDLQFTPGLNVVVHQVEVDNKPLGLWNFVTSEGSCGGSMVGAKESSASSTARHFNGLGYAQLMKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGRIMFRIDYGDESKLEINTTKKYNVGQWIKIEAAREFSAKRSTENGMLRVNNDRPISGAPTLPVNIHLLPDLSKTVYYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSYYGVEPSCKNMITKAGFSGNGYLELPSQSLRKRSNTALVFRTLQPDCLLLLAAYPPEILGDYDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQEIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFNNRTINFNDALTFANVQIGRNGPLMGSLKGGLYDVLLKTEPMIGKSFTASPEGCKRDFSC

Target ORF information:

RefSeq Version	NM_057442.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wing blister (wb), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057442.5

ORF Insert Sequence:

ATGCAAATCG GAAATTTTAT CAAATGGCCA CTGGGCCTGA TCCTCATCCT GCTGGTCACC 
CACTGTCAGG CCAAAAGGCA GCGGAAACGG TTGCGTGGAT TCTCCACTGC CAAGGACCTA 
CAGGATTATA GCATGCCGCT CAACGACAAC TCCACGGAAC TCCAGCAGAA CATTGTGTCC 
TGGACAGGAG CTCGAATTGC GTCGAGTCCC GATAAGAAGA AGAAGTCACC GCAATCATCG 
AGACGTCACA AAACCAAAAG TGGCCTGATC ATTGAAAGTA ACATTGCCGA CTCGGAATCG 
ATGTTGGCTA TTGCCTCCTC GGAATCCGTC ACCAAGAAGA AGCCGCCGCA GCGTCGTCGC 
AACGGAAAAT TACAGAAATC CGGAGGAGGA GCAGGAGGTG GAGCCCTTCG ATTGCTCAAA 
ACGGAGGACG TGTACTCATC CAGTTTCTCC GGCGGCCTCT ATCCGCCGCT CTTCAATGTG 
GTTCCACGGG CTCAGATCTC GGTGAATGCC ACTTGTGGCC AGAACGGTGC CGAGGAGTAC 
TGCAAACAGG TCGGCGCCAA GCCGTGTGGC ATCTGCAATG CCCATTCCTC GGATCGAGCC 
AAACAGCGCT CCATACAGTC GCTGATATCG TCGGGATCGG GATCGGGATC GGGATCGGGA 
TTTGAAGAAG GCTGGTGGCA ATCACCAACG CTGCAGGGTG GCCGGCAATT TGAGTATGTG 
ACCATACTAT TGGATCTGAA GCAGACTTTT CAAATATTTT CCGTGTGGCT AAAATCGGCC 
AACTCTCCGC GTCCAGCCTC ATGGATACTG GAAAAGTCCC TGGATGGGAT TAACTTTGAG 
CCATGGCAGT ATTTCGGATT GAGCGATGCA GATTGTCAGC GACGATGGAA TTTATCCGGG 
CAAAATGGCA AATACGTCTT CCAGAACGAT ACAGAAATCA TTTGCTCCAC TCAGTTTTCG 
AAGCCGGGTC CTTTGGAGAA CGGCGTCTTG CATGCTTCGC TGCTGAAGAA TCGCCCTGGA 
GCCACGGATC AAAGTCCGGA GCTAATGAAG TTCATAACGA CTCGGTACAT AAGGATTCGC 
CTGCAGGGCA TGCACTCCAC GGCCAATCAG GACAACAGTC TGGACTGGCT GCTGGACTCT 
CCGTCGCTGG AGAAGCACAG CTTCTACTCG CTGTCCCAGT TGAAGGTGAG TGCCCGTCTG 
GATTGTAATG GGCACGCCAA TCGCTCCCAC GAGTCGCCAG ATGACCCTCT GATGCAGTGC 
ATCTGCCAGC ACAATACCTG TGGTGCTCAG TGCGAACAGT GCTGTCCGCT TTTCCAGGAT 
CGCCCCTATC AAATGGGCGG AGAGTGCGAG ATATGCCAAT GCTATGGTCA CGCCGAGAGC 
TGCACCTATG ATCCCTTCCT GGACAAGGGC ATTTGCCAGA GCTGCTCGAA TAACACGGCT 
GGCATTGAGT GCGAGTTTTG CGAGATGGGA TTTTACAGGG AGCTGGATGC ACCGCTCACC 
GATCCCTGTT TGCCCTGCTC CTGTAATCCT GCTCGTTCCA CGGGTGGTTG CCAGTCGGAT 
GGAGGAAGCT GTAACTGCTT GGAGGGTTTC CAGGGCAAGA ACTGCGAGGA ATGTGCTCCG 
GGCTACTACG GAGACGATTG CAAACGGTGT GAGTGCGACG AAAGGGGAAG TCTTGGCTCC 
ACGGGTTCCT GCTCGGGTGT GTGCCAGTGC AAGCTCAATG TGGAGGGCAG CACCTGCTCC 
GAGTGTGCGC CCGGATATTT TGATCTAAGC GCAGAGAATG CAGAGGGATG CACCAGCTGT 
TGGTGCTCAG GTGTCTCGCA AACATGTCAC AGTGCCAAAC TGCAGACATT GGCCTTTGAA 
ACCCTCAACG ATTGGAAAAT AACCGATATT CAAAGAGTGA AACCAATTTC AATACCAGTT 
GATGCCGAAA CAAACCGTTT GATCTTTGCC AACGAACTGG ATGAAGTGGA GGCCATTTAC 
TGGCAGGCTT CGTTGGGTTA CTTGGGAAAT CGCTTGACCA GCTATGGGTC GAGACTGCAG 
CTGGTGTTGT CGTGGGATGT GATAAGAGGC GATAGATCGG GAAAGCCCAC GACGGGGCCC 
AATGTCATCC TAGTCGGCAA GAATGGACTG AAGATTGCCT TCGGCGATGA ATCCTTGGAC 
GGATTGGGAA TCAACTTGAA TGTAACCCTA ACAGAGGTTG GCTGGTATCA CGTGCCACCC 
ACTGTCGTTG ATATAAAAAC AAGACTTCGG CGAACCGAGG GCGGTGACTA TCACGGAGAA 
TCTGTTACCC GTTCCCAGTT TCTCTCCGTT CTCGTTTCCC TCGATGCTGT CCTTATTAGA 
GCGGCTTTTC ACACGGATCA AGGGGAAACA TCACTCGAGA GAGCAGTCAT CTATTCAGGA 
GGTGTGGAGT TGGGCGGAAA GTCTTCCAGC CAAGTGGAGC AATGCCTCTG CCCAGCTGGC 
TATACCGGTC TGTCCTGCGA GGGCTGCGCC TTTGGATTCA AGCGGATTTA CGAGAACACC 
TCGGACCATC AGATTCTGAG CAAGTGCATC CCGTGTCCCT GCAACGGACA CTCAAACTCC 
TGCGATCTGC AGAGCGGAAA TTGCGGCGAC TGCATGCACA ACACTTTTGG AGATCGTTGT 
GAGCGTTGCC AGTTGGGTTA CTACGGGAAT CCCCTGCAGG GAACCCCCAA TGACTGCAAG 
CGATGCGCCT GTCCCCTGTC CGAGGATTCC AACAACTTCT CGCCCAGTTG TCAGCTAAAG 
AGCTACAACT ACATGGACCT GAATCCGCAG TTCGAGCTGA TAGAGCATGC GGAGTACATA 
TGCACCCAAT GTCCGGAAGG TTATACGGGT GATCATTGTC AGGTGTGCGA CGATGGGTAC 
TTCGGGAATC CACGCCAGCC GGGAAGCAGT TGTCAGCGCT GCGATTGCGC CGGAGGGCCC 
TGCAACGTGA CCACCGGGGA GTGCATCACG TGCCGCGGAA ACACAGAGGG TTGGCACTGC 
GAGCGCTGCA AGCTGGGCTA CTGGGGTGAT CCGGCCGTGG GCTGTGATCC CTGTCATTGC 
CACACGGAAG GATCGGAGAG CGGACTATGC GACAGCACCG ATGGTCAATG CCTGTGTAAG 
CCACGTTATG CTGGACAGAA GTGCGATGAA TGTGACGTGG GCTATGCTAA TGTTGAGCTA 
CGCTGTCCAT CCTGCAACTG CGATCCATTG GGCTCGTTGG TCCAGGACAG ATGTGATCCC 
CACACTGGAC AATGCCATTG TAAGGAGGGC GTTATGGGTG CCAAATGCCA CGAGTGCCAG 
GATGGCTACT TCGGCATGAA TGCTGTGGCC TATCGAATGG ATGACCTTGC TGCACTGCGT 
CAAAACTCCG ATAGCGACGA TGACGAATGG GAATTGGTTC CAGATACTGA AGATCCCAAT 
AGCGAATCCA CGGTGGCCTG CGAGGAATGC CACTGCAGCA GTGTGGGATC CCTGTCATCG 
GATTGCGATA AGCGAACTGG GCAATGCGCC TGCCTGGCCA ACGTCACCGG GAGGAGATGC 
GACAAGTGTC GACCGGGTCA CTGGAATCTC ACGGCGGGCG AGGGCTGCCG GGATTGCCGA 
TGCGATCCTC ATGGTTCCCG AGGTCACGAG TGCAACCCGT GGACGGGCCA ATGCGACTGC 
AAGATCGGGG TTGGAGGTCA GCACTGTAAC GAGTGTACGG AGGGATTCTT TGGCTTCAGC 
ACGGAGGGTT GTCAGCGGTG CTCGGCCTGC AGGTCGGAGG GTCAGGTGTG TGACCCGCAC 
AATGGACGCT GCATTTGCCC CAAGTTCACC CGTGGCCTGG GATGTGGACA GTGTGTGCCG 
GGCACCTGGG GCTGGCAGGC TCGATTGGGT TGCCGGGAAT GCGAGTGCGA TCACATTGGC 
TCCATTGGTC AGCAGTGCAG CACCGGCGAT GGACAATGCC AGTGCCGAGA GGGATATTCG 
GGCAGAAAGT GCGACACTTG CGCCATCGGG TATTTCGGCT ATCCGGAGTG CCGACGATGC 
GGCTGCGATG CGGAGGGTTC CTTCACCCAG GCGGATGGCT CAATAGCCTG TGACTCGAAT 
GGCCAATGCC CGTGCAAATC CCTTGTGGTG GGTCTCAAAT GCGATACCTG CATGCAAAGC 
ACTTTTGGAT TGTCCGCACA AAATCCCGAA GGATGCACCC GATGCTTCTG CTTCGGGAGA 
TCCGGTGAGT GCGAGCAGAG CGACTTGTCT TGGGGACACA TCCGTATGGC GGAGTCCAGA 
AATCTCAGCG TTCAGCAAAT AAGACCGCAC TCGGTACCCA GCTCAGACTA CGAGTACATA 
GTGGTGGTTC AGATGGAGGG CAGCAGTTTC CATCGCGAAG ACGCGGAGAT CCAGCGCATG 
AATGATCTGA GCTTAGTGCC CAAATCTACG GGGAATGTTT CCATCGGAGC ATATGGACAA 
TTTTACCATC CGCTCTACTT CCAACTGCCT CCGCAGTTCT ACGGAGATCG GACTAGTAGC 
TATGGTGGCT TTTTGTACTT CACCCTGATC ACCGAAGGTG CCCACAAGCC CTTGGAGCGA 
AATATTTTGG GTCAGTATCC ATTGGTTCAG TTACACGCGC ACTCCAAGTT GCTACTGGAC 
TTTTACGAGT ACGAAGAGTT CGAGTACTCC TTGAACGTGA CCCATCGGGT GCCGCTGCAT 
GAGTCCTTCT GGAAGTACCA CCACACCAGC CAGGCAGTGG ATCGTAATAC TCTAATGGCT 
GCCCTCCAAA ACATTCGACA CATCTTCATT CGAGCCTTTG CTTTTGCCGA TTTCCAGGAA 
GTTGTCCTTC AAAATGTACA CATGGATGCG GCCATCTATA TCAAGGGCTC CACCAATCTT 
ATAGCCAAGG GAGTCGAACG GTGTAAGTGT CCCAAGCGGT TTGATGGACT TTCATGCCAG 
GATCCGGGAA GATCCTTCTA CCGTTGGCGG AATACCACTA TTGTGGAGAG TGTATTCATT 
GAGGATCTGA TAGGAAGAGC AGCTCCCTGT CATTGTAATG GTCGTAGCAG CGATTGTGAT 
CGCGAAACTG GAGTCTGTCA GAACTGTCGA GGTAACACTG GAGGAGATCA CTGCCATCAG 
TGTGCTGAGG GATTCTACGG CGATCCCAAT TCTCCACACG GTTGTCAGGC TTGTCCGTGC 
CCGGAGACCA ATAGGAACTT TGCCCGGGGC TGCAATGTCT GGGACGGAGA AGTCAGTTGT 
GTCTGCAAGC CGGGTTACAC GGGCAGATTG TGTGAGCGGT GCCAGGCGGG ATATTTTGGA 
GATCCTATGA GATATCCAAA CACTACTTGC CAACCTTGCA ACTGTCACCC AGATGGAATC 
CAAACCGAAG GCTGCGATGT AGAGACTGGT AGATGCTACT GTCGAGAAGG AGTTACTGGG 
CTAAAGTGCG ACAAGTGTCA GGCGGAACGT CATCATCTGG TGGACAACGG ATGCAAAATC 
TGTGACAATT GCACTCTTCT GCTTCTGGAT TATATGGAGC TGGTGGGCAA CAAGCTTCGT 
CGTGGAATGC ACAACATGGA TTTGACTGGA ATTCCGGCGC CATATCGAAA ATTGTCAGAG 
TACGAAAGCG CCTATGAGAA ATGGAATGGG CGCCACTGGG ACTTTAGCCA GACCAAACGG 
CGTCTACAGG ATTACGACTC CGCTGACATC CTAAAGCTGG AGGCCCACGC CGAAAATTTG 
AAGTTTCAAT CCCGCAAAGC AGTGGCCACC ATTGGAAAAC GGGAGTTTGC CATAAAATCG 
ATGCGGGAAG ATGCTGTAAC CCAACAGCAT AGTGTCGGCT TACTCCGCTC GGAGATTCTT 
CAAACCCTTT CCGATTTACA TGGCTATGGA AAGAGTGCCC ATTATCTCAG TCTGCCTACT 
GCTCTCAAGC AGGCCCGATT CTACCTGCAG GCCATCCGGG AGCACGACCA AATGGTTCAG 
GGAATCCGCA GTACCAACGA CTGTGCATGG AAGCATTTTT ATGCCATGGG CAACGCATCG 
GATGCGTCGT TCGACGAGAG CGGCCGCTTG GAGATGCTCT GGCGGGACTT GAATCAGACC 
AATCATCGAG TGGTAGACAT GCGGCTGCAG GTGGATCGCG TGCAAGAGGT GGAGAACGAA 
GCGGAGGACG TTCTGGAACA TGTGCGCAAT TTGAGTATTC GCGTGGGTGA ATCCCACCAG 
GAGCTGGATG AACTTAACCA GCGTATTTCC GATCACCTAG ATCCGGGCTA CCTAGAACAG 
GGCGAGGGCC TGCTAAGGCT GACAGTCCAG CGGCAGATAA TGCTCAATGG GCATCTTAAC 
CAATTGGATG GCTACCGTAT ACTACTAAAT ACCACATTGG GCGTAAAGAC TGAGCAGCAG 
CGGGAGGTGC GTAAACACTG GCTTCCCAAG GCGGAAAAGC ACGCCAGTCA TCTGCTGGCG 
CGATCCAATG AGTACGCCCG CAAGTTTCAA CCCACCAGAA ATGGAGCCCG CATAGCCATG 
CTGGCCAGTT CGGCCCACAG CAATATCACT GAGGCCATCA ACGATGCCCG GCTAGCATCA 
ATCCTGGCCA AGGAACGGGT GTACGAGGCG CAGAGAACAC TCTATCCCAG TGACGGCAGC 
TCCATGATCG AGCGAGCCAA GCACTCGCTG CACAGATCAA AGCAACTTCA GCAAGAAGCG 
CTCAAGCAGA TGCACAAATC CAATGTGTTG AAGGACAAAC TGCATCGCCA GGAGCAGCAG 
GTGGAGGGCA TCAAGTCCAC GATTTACGAT TCCGGACTGC GCACCAACAA CATATCCGGA 
CAGCTGCAGG GTCTTTCAGA TGGTTCAGCA CGTCGTCAAG CGAAGGATAG TCTGGAAATG 
GCTGATAGAA CGGGAGAACA AATGCGTGCT GAACTGCAAA AGGCGAAGGA TATGCAGAAG 
TCCATCCAAA ACATGCGAAA TTCGTTCTCC AATCTGGAAC CCGACTGGGA AATCAAGCTG 
GGAATGGCCC AAGAGAACAT ATCCCTGACC CAGACTAACT TGCGTCTGGC CAATGTCTCA 
TTGAGCTACC TAGAGCAGCA GGCCGAGAAG GAGCAACAGG TGTTCGAGGT GTGGAACAAC 
AGCATGGCCC AGCAACTGCA GCAGCTCCGG GATCAAATAG CCAAGGCCAG ACATGCAGCA 
GAAGCGATCG ATGTTTCCCT GGAATCGCTG GGTCCTAAAT GCATTCGATC CTATTTGCCC 
GCCTCCTACG GTTTGAGTAC CTCGAATAAG ATTCGTATGA GCTTCGCCTT ATCCAATCAC 
CTCGAGAGCT CTCCATTGAT TCACCTGGCC AGCAGCGAGG GGCGTCACAT CACCCTGGAG 
CTGTACAAAC GTCGTGTGCG CCTGGTGTGG AATCTTGGTG GCACCACCGC AACCGTTACG 
CATCCCATGG TGGTGCATAC TCGTGACCCC AAGTACGACG ATGCCTGGTA CCATGTGGAG 
GCCAATAGGA CACTTAATCT GGGCAGCCTG GTCGTGCGTC GAATGAACAA CTATGGCGAG 
CTGACTCCGC CTAACCCGGT CATCATAACA GGATCCACGG ATACGGAGCA CACACGTTTC 
TATCAGTCTC GAAGCGATCG AATTTCGCTG GGAGGCTTTG CATCGAAAGA TTTGCAATTC 
ACGCCGGGAC TCAATGTGGT GGTGCATCAG GTGGAGGTGG ACAACAAACC GCTGGGTCTG 
TGGAACTTCG TGACCAGCGA GGGCAGCTGC GGTGGATCAA TGGTTGGAGC TAAGGAGTCT 
TCGGCCTCGT CCACGGCTCG CCATTTCAAT GGATTAGGCT ATGCCCAGTT GATGAAGACC 
CGACCACGAC CCACTCGCAA AAACCTGTTC TCCGTGCAGA TGACTTTCCG CACGTTGGAC 
GAAAATGCCT TGCTCTTCTT GGCGGTTGAT GACAAGAACA ACCGCTCGGT ATCGGTAACT 
TTGAGCCGGG GTCGGATCAT GTTCCGCATT GACTATGGCG ATGAGTCCAA GCTGGAAATC 
AACACCACTA AGAAGTATAA TGTGGGCCAG TGGATCAAGA TCGAGGCTGC GCGAGAGTTC 
TCGGCGAAAA GAAGTACTGA AAATGGAATG CTCCGAGTAA ACAACGATCG TCCAATTTCC 
GGAGCACCTA CACTACCCGT AAACATTCAC TTGCTTCCGG ATCTTTCCAA GACTGTCTAT 
TATCTGGGCG GTGTGCCACC AGGTTTCACT TCGGGAACCT CAAAGGCGCC TGGTGCTGAT 
AATCCCTTCT TGGGATGTAT GAAGGATGTC CAGGTAAATG GCGAGACCTA CGATCCTCTG 
GAGAGCTCAA GCTACTACGG TGTGGAGCCT TCCTGCAAGA ACATGATTAC CAAGGCTGGT 
TTCTCGGGCA ATGGTTACTT GGAGCTGCCA TCGCAGTCAC TACGAAAGCG CTCCAATACC 
GCTTTGGTGT TCCGCACCCT TCAGCCCGAT TGCCTGCTGC TCCTTGCTGC CTATCCGCCT 
GAGATCCTGG GCGACTATGA TGCTAAGGAC ATTAAGGGCA ACTTCTCCAT TAGCCTGGTC 
GATGGTCAAT TGCATGTTTG GGTTAACTCT GGTCGCAGCT TCATCAAGAT GTCCTCGAAC 
TCCAGTCAGA TGAACGATGG CGAGTTTCAC GTGGTACATT TGATCAAGAC CGGTCGCAAG 
CTGGAGCTGA TGGTGGACGA TGAGCTGCAA GAGATTCGCA ATCTAAACGG CAATCCCACG 
GTGGTCAGCC TGCCGAGGGA TGCAGGTGGC CTGTATATAG GTGGAGCTCC GCCGCACGAA 
AGTTATACGC CATTGGCGCC GACTTTTGTA AATCTAGAAG GAGCCATTCG GGATGTGGTG 
TTCAACAATC GGACCATTAA CTTTAACGAT GCCCTGACCT TTGCCAACGT CCAGATTGGA 
CGTAATGGTC CACTGATGGG CAGTCTGAAA GGAGGACTGT ACGATGTCCT CCTGAAAACC 
GAACCCATGA TTGGAAAGAG TTTTACGGCC TCGCCTGAGG GCTGCAAGCG GGATTTTAGT 
TGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wb ( NM_001201877.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188806.1 Drosophila melanogaster wing blister (wb), transcript variant C, mRNA.

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CloneID	OFa15831
Clone ID Related Accession (Same CDS sequence)	NM_001201877.2 , NM_001201879.2 , NM_001201878.2 , NM_001201880.2
Accession Version	NM_001201877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wing blister, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201877.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188806.1
CDS	761..8464
Translation	MSASDKRTVKMSDASIKPRKLKKHLRAADVKPRGMMTKSLLLHLCVYLVCITQTLQLQQRQQQQQQQHRQQQHGQPEGNMLQQHHRTSVNSSVNINISRQFNEIIQTATASASASGTGSASGAVTFASVTAATTITPGRRKLRRKFQGQQNRNSTLGRRRKLRLHPYHRRNATHATRTTTVASVSATATTLLPKMPHKQPFNYHTHLHSSFQQQQQQQHVAGGQVTQQQQQQQQVHVVAVTPRPQQRQQQPGFAIVPATYYHQQQQQQQRTLLGNLTQQQQQQQQHLLIQPGQNILVYSNLGPIPPCPPGLLSGSGMPVYTQQQHFIPLRSTTTPRPRQRECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQARFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQTNHRVVDMRLQVDRVQEVENEAEDVLEHVRNLSIRVGESHQELDELNQRISDHLDPGYLEQGEGLLRLTVQRQIMLNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEKHASHLLARSNEYARKFQPTRNGARIAMLASSAHSNITEAINDARLASILAKERVYEAQRTLYPSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISGQLQGLSDGSARRQAKDSLEMADRTGEQMRAELQKAKDMQKSIQNMRNSFSNLEPDWEIKLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEVWNNSMAQQLQQLRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHLASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAWYHVEANRTLNLGSLVVRRMNNYGELTPPNPVIITGSTDTEHTRFYQSRSDRISLGGFASKDLQFTPGLNVVVHQVEVDNKPLGLWNFVTSEGSCGGSMVGAKESSASSTARHFNGLGYAQLMKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGRIMFRIDYGDESKLEINTTKKYNVGQWIKIEAAREFSAKRSTENGMLRVNNDRPISGAPTLPVNIHLLPDLSKTVYYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSYYGVEPSCKNMITKAGFSGNGYLELPSQSLRKRSNTALVFRTLQPDCLLLLAAYPPEILGDYDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQEIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFNNRTINFNDALTFANVQIGRNGPLMGSLKGGLYDVLLKTEPMIGKSFTASPEGCKRIGSYSYEPNAFKFGDDIYSYSQLKLPERHFWQRNFHLSFDFRSFYPNGMLYLSPGSKEKPKHYVALVLKDGQLVLVVRGRRREELQLTAKLNDGEWHRVTISCHDRKVTMSVEIGRTDQKTSAQMKLPKKIGASQLLLVGGLPQSPVKVSSDLYVRLEPFKGCLRRVSINNNTQDLARPGKHSNVGQCFPTVERGSYFPGDAYAIYKKNFNVGKYLDLETEFRTSELSGILLSVSDPNGFPALSLELHNGNIIFSCDPGNGAPMRVESSLPTKYALCDNKWHNISALYDGEQIVLRIDQLPAVISVGNQGNAGKVQTRSPLYIGGLPESAPSGSLISRENFKGCIRHVSIRNERRDWIEMEDLRNVLLSECLVSSDES

Target ORF information:

RefSeq Version	NM_001201877.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wing blister (wb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201877.2

ORF Insert Sequence:

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wb ( NM_001201879.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188808.1 Drosophila melanogaster wing blister (wb), transcript variant E, mRNA.

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CloneID	OFa15831
Clone ID Related Accession (Same CDS sequence)	NM_001201877.2 , NM_001201879.2 , NM_001201878.2 , NM_001201880.2
Accession Version	NM_001201879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wing blister, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201879.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188808.1
CDS	969..8672
Translation	MSASDKRTVKMSDASIKPRKLKKHLRAADVKPRGMMTKSLLLHLCVYLVCITQTLQLQQRQQQQQQQHRQQQHGQPEGNMLQQHHRTSVNSSVNINISRQFNEIIQTATASASASGTGSASGAVTFASVTAATTITPGRRKLRRKFQGQQNRNSTLGRRRKLRLHPYHRRNATHATRTTTVASVSATATTLLPKMPHKQPFNYHTHLHSSFQQQQQQQHVAGGQVTQQQQQQQQVHVVAVTPRPQQRQQQPGFAIVPATYYHQQQQQQQRTLLGNLTQQQQQQQQHLLIQPGQNILVYSNLGPIPPCPPGLLSGSGMPVYTQQQHFIPLRSTTTPRPRQRECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQARFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQTNHRVVDMRLQVDRVQEVENEAEDVLEHVRNLSIRVGESHQELDELNQRISDHLDPGYLEQGEGLLRLTVQRQIMLNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEKHASHLLARSNEYARKFQPTRNGARIAMLASSAHSNITEAINDARLASILAKERVYEAQRTLYPSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISGQLQGLSDGSARRQAKDSLEMADRTGEQMRAELQKAKDMQKSIQNMRNSFSNLEPDWEIKLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEVWNNSMAQQLQQLRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHLASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAWYHVEANRTLNLGSLVVRRMNNYGELTPPNPVIITGSTDTEHTRFYQSRSDRISLGGFASKDLQFTPGLNVVVHQVEVDNKPLGLWNFVTSEGSCGGSMVGAKESSASSTARHFNGLGYAQLMKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGRIMFRIDYGDESKLEINTTKKYNVGQWIKIEAAREFSAKRSTENGMLRVNNDRPISGAPTLPVNIHLLPDLSKTVYYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSYYGVEPSCKNMITKAGFSGNGYLELPSQSLRKRSNTALVFRTLQPDCLLLLAAYPPEILGDYDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQEIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFNNRTINFNDALTFANVQIGRNGPLMGSLKGGLYDVLLKTEPMIGKSFTASPEGCKRIGSYSYEPNAFKFGDDIYSYSQLKLPERHFWQRNFHLSFDFRSFYPNGMLYLSPGSKEKPKHYVALVLKDGQLVLVVRGRRREELQLTAKLNDGEWHRVTISCHDRKVTMSVEIGRTDQKTSAQMKLPKKIGASQLLLVGGLPQSPVKVSSDLYVRLEPFKGCLRRVSINNNTQDLARPGKHSNVGQCFPTVERGSYFPGDAYAIYKKNFNVGKYLDLETEFRTSELSGILLSVSDPNGFPALSLELHNGNIIFSCDPGNGAPMRVESSLPTKYALCDNKWHNISALYDGEQIVLRIDQLPAVISVGNQGNAGKVQTRSPLYIGGLPESAPSGSLISRENFKGCIRHVSIRNERRDWIEMEDLRNVLLSECLVSSDES

Target ORF information:

RefSeq Version	NM_001201879.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wing blister (wb), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201879.2

ORF Insert Sequence:

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wb ( NM_001201878.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188807.1 Drosophila melanogaster wing blister (wb), transcript variant D, mRNA.

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CloneID	OFa15831
Clone ID Related Accession (Same CDS sequence)	NM_001201877.2 , NM_001201879.2 , NM_001201878.2 , NM_001201880.2
Accession Version	NM_001201878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wing blister, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201878.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188807.1
CDS	843..8546
Translation	MSASDKRTVKMSDASIKPRKLKKHLRAADVKPRGMMTKSLLLHLCVYLVCITQTLQLQQRQQQQQQQHRQQQHGQPEGNMLQQHHRTSVNSSVNINISRQFNEIIQTATASASASGTGSASGAVTFASVTAATTITPGRRKLRRKFQGQQNRNSTLGRRRKLRLHPYHRRNATHATRTTTVASVSATATTLLPKMPHKQPFNYHTHLHSSFQQQQQQQHVAGGQVTQQQQQQQQVHVVAVTPRPQQRQQQPGFAIVPATYYHQQQQQQQRTLLGNLTQQQQQQQQHLLIQPGQNILVYSNLGPIPPCPPGLLSGSGMPVYTQQQHFIPLRSTTTPRPRQRECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQARFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQTNHRVVDMRLQVDRVQEVENEAEDVLEHVRNLSIRVGESHQELDELNQRISDHLDPGYLEQGEGLLRLTVQRQIMLNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEKHASHLLARSNEYARKFQPTRNGARIAMLASSAHSNITEAINDARLASILAKERVYEAQRTLYPSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISGQLQGLSDGSARRQAKDSLEMADRTGEQMRAELQKAKDMQKSIQNMRNSFSNLEPDWEIKLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEVWNNSMAQQLQQLRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHLASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAWYHVEANRTLNLGSLVVRRMNNYGELTPPNPVIITGSTDTEHTRFYQSRSDRISLGGFASKDLQFTPGLNVVVHQVEVDNKPLGLWNFVTSEGSCGGSMVGAKESSASSTARHFNGLGYAQLMKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGRIMFRIDYGDESKLEINTTKKYNVGQWIKIEAAREFSAKRSTENGMLRVNNDRPISGAPTLPVNIHLLPDLSKTVYYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSYYGVEPSCKNMITKAGFSGNGYLELPSQSLRKRSNTALVFRTLQPDCLLLLAAYPPEILGDYDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQEIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFNNRTINFNDALTFANVQIGRNGPLMGSLKGGLYDVLLKTEPMIGKSFTASPEGCKRIGSYSYEPNAFKFGDDIYSYSQLKLPERHFWQRNFHLSFDFRSFYPNGMLYLSPGSKEKPKHYVALVLKDGQLVLVVRGRRREELQLTAKLNDGEWHRVTISCHDRKVTMSVEIGRTDQKTSAQMKLPKKIGASQLLLVGGLPQSPVKVSSDLYVRLEPFKGCLRRVSINNNTQDLARPGKHSNVGQCFPTVERGSYFPGDAYAIYKKNFNVGKYLDLETEFRTSELSGILLSVSDPNGFPALSLELHNGNIIFSCDPGNGAPMRVESSLPTKYALCDNKWHNISALYDGEQIVLRIDQLPAVISVGNQGNAGKVQTRSPLYIGGLPESAPSGSLISRENFKGCIRHVSIRNERRDWIEMEDLRNVLLSECLVSSDES

Target ORF information:

RefSeq Version	NM_001201878.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wing blister (wb), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201878.2

ORF Insert Sequence:

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wb ( NM_001201880.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188809.1 Drosophila melanogaster wing blister (wb), transcript variant F, mRNA.

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CloneID	OFa15831
Clone ID Related Accession (Same CDS sequence)	NM_001201877.2 , NM_001201879.2 , NM_001201878.2 , NM_001201880.2
Accession Version	NM_001201880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wing blister, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201880.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188809.1
CDS	782..8485
Translation	MSASDKRTVKMSDASIKPRKLKKHLRAADVKPRGMMTKSLLLHLCVYLVCITQTLQLQQRQQQQQQQHRQQQHGQPEGNMLQQHHRTSVNSSVNINISRQFNEIIQTATASASASGTGSASGAVTFASVTAATTITPGRRKLRRKFQGQQNRNSTLGRRRKLRLHPYHRRNATHATRTTTVASVSATATTLLPKMPHKQPFNYHTHLHSSFQQQQQQQHVAGGQVTQQQQQQQQVHVVAVTPRPQQRQQQPGFAIVPATYYHQQQQQQQRTLLGNLTQQQQQQQQHLLIQPGQNILVYSNLGPIPPCPPGLLSGSGMPVYTQQQHFIPLRSTTTPRPRQRECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHGSRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFTRGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYPECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGRSGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSLVPKSTGNVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNILGQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHIFIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTTIVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTGGDHCHQCAEGFYGDPNSPHGCQACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGIQTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHNMDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQDYDSADILKLEAHAENLKFQSRKAVATIGKREFAIKSMREDAVTQQHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQARFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQTNHRVVDMRLQVDRVQEVENEAEDVLEHVRNLSIRVGESHQELDELNQRISDHLDPGYLEQGEGLLRLTVQRQIMLNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEKHASHLLARSNEYARKFQPTRNGARIAMLASSAHSNITEAINDARLASILAKERVYEAQRTLYPSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISGQLQGLSDGSARRQAKDSLEMADRTGEQMRAELQKAKDMQKSIQNMRNSFSNLEPDWEIKLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEVWNNSMAQQLQQLRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHLASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAWYHVEANRTLNLGSLVVRRMNNYGELTPPNPVIITGSTDTEHTRFYQSRSDRISLGGFASKDLQFTPGLNVVVHQVEVDNKPLGLWNFVTSEGSCGGSMVGAKESSASSTARHFNGLGYAQLMKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGRIMFRIDYGDESKLEINTTKKYNVGQWIKIEAAREFSAKRSTENGMLRVNNDRPISGAPTLPVNIHLLPDLSKTVYYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSYYGVEPSCKNMITKAGFSGNGYLELPSQSLRKRSNTALVFRTLQPDCLLLLAAYPPEILGDYDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQEIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFNNRTINFNDALTFANVQIGRNGPLMGSLKGGLYDVLLKTEPMIGKSFTASPEGCKRIGSYSYEPNAFKFGDDIYSYSQLKLPERHFWQRNFHLSFDFRSFYPNGMLYLSPGSKEKPKHYVALVLKDGQLVLVVRGRRREELQLTAKLNDGEWHRVTISCHDRKVTMSVEIGRTDQKTSAQMKLPKKIGASQLLLVGGLPQSPVKVSSDLYVRLEPFKGCLRRVSINNNTQDLARPGKHSNVGQCFPTVERGSYFPGDAYAIYKKNFNVGKYLDLETEFRTSELSGILLSVSDPNGFPALSLELHNGNIIFSCDPGNGAPMRVESSLPTKYALCDNKWHNISALYDGEQIVLRIDQLPAVISVGNQGNAGKVQTRSPLYIGGLPESAPSGSLISRENFKGCIRHVSIRNERRDWIEMEDLRNVLLSECLVSSDES

Target ORF information:

RefSeq Version	NM_001201880.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wing blister (wb), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201880.2

ORF Insert Sequence:

ATGTCAGCGT CTGACAAACG AACTGTCAAA ATGTCAGACG CGTCAATAAA GCCACGAAAA 
TTAAAAAAAC ATCTGCGGGC AGCAGATGTA AAGCCTCGTG GCATGATGAC AAAAAGCCTG 
CTGCTGCATC TATGCGTCTA TCTAGTCTGC ATAACACAAA CGCTGCAACT GCAGCAACGA 
CAACAGCAGC AGCAGCAGCA ACACCGACAG CAGCAACATG GCCAGCCCGA GGGCAACATG 
CTGCAGCAAC ATCACCGAAC CAGTGTAAAT AGCAGTGTAA ATATCAATAT AAGCAGGCAA 
TTCAATGAAA TTATACAAAC GGCAACGGCC AGTGCATCGG CCAGCGGTAC GGGATCGGCA 
TCGGGAGCGG TAACATTCGC ATCGGTTACG GCAGCAACAA CAATAACGCC AGGACGTCGA 
AAACTGCGAC GCAAGTTTCA GGGGCAACAA AACCGTAATT CCACCTTGGG CAGGAGGCGA 
AAACTGCGTT TGCATCCCTA TCATCGGCGC AATGCAACAC ATGCCACACG CACCACGACT 
GTGGCAAGTG TCTCTGCGAC GGCAACTACG CTGCTACCAA AGATGCCCCA CAAGCAGCCC 
TTCAACTATC ACACCCACCT GCACTCGAGC TTCCAGCAGC AGCAACAGCA GCAACATGTT 
GCCGGTGGCC AAGTTACCCA GCAGCAGCAG CAGCAGCAAC AAGTGCATGT TGTTGCCGTA 
ACACCGCGCC CGCAACAGCG GCAACAACAG CCCGGATTTG CCATAGTACC TGCAACCTAC 
TATCACCAGC AGCAGCAGCA ACAGCAACGA ACACTTTTGG GCAATCTCAC CCAGCAGCAG 
CAGCAACAGC AGCAACATCT ACTCATACAG CCGGGCCAAA ACATTTTGGT CTATAGCAAT 
CTGGGACCCA TACCGCCCTG TCCGCCGGGC CTGCTTTCCG GATCGGGAAT GCCAGTCTAT 
ACGCAACAGC AACACTTTAT ACCGCTGCGA TCCACGACGA CCCCGCGCCC CAGGCAACGT 
GAATGCCACT GCAGCAGTGT GGGATCCCTG TCATCGGATT GCGATAAGCG AACTGGGCAA 
TGCGCCTGCC TGGCCAACGT CACCGGGAGG AGATGCGACA AGTGTCGACC GGGTCACTGG 
AATCTCACGG CGGGCGAGGG CTGCCGGGAT TGCCGATGCG ATCCTCATGG TTCCCGAGGT 
CACGAGTGCA ACCCGTGGAC GGGCCAATGC GACTGCAAGA TCGGGGTTGG AGGTCAGCAC 
TGTAACGAGT GTACGGAGGG ATTCTTTGGC TTCAGCACGG AGGGTTGTCA GCGGTGCTCG 
GCCTGCAGGT CGGAGGGTCA GGTGTGTGAC CCGCACAATG GACGCTGCAT TTGCCCCAAG 
TTCACCCGTG GCCTGGGATG TGGACAGTGT GTGCCGGGCA CCTGGGGCTG GCAGGCTCGA 
TTGGGTTGCC GGGAATGCGA GTGCGATCAC ATTGGCTCCA TTGGTCAGCA GTGCAGCACC 
GGCGATGGAC AATGCCAGTG CCGAGAGGGA TATTCGGGCA GAAAGTGCGA CACTTGCGCC 
ATCGGGTATT TCGGCTATCC GGAGTGCCGA CGATGCGGCT GCGATGCGGA GGGTTCCTTC 
ACCCAGGCGG ATGGCTCAAT AGCCTGTGAC TCGAATGGCC AATGCCCGTG CAAATCCCTT 
GTGGTGGGTC TCAAATGCGA TACCTGCATG CAAAGCACTT TTGGATTGTC CGCACAAAAT 
CCCGAAGGAT GCACCCGATG CTTCTGCTTC GGGAGATCCG GTGAGTGCGA GCAGAGCGAC 
TTGTCTTGGG GACACATCCG TATGGCGGAG TCCAGAAATC TCAGCGTTCA GCAAATAAGA 
CCGCACTCGG TACCCAGCTC AGACTACGAG TACATAGTGG TGGTTCAGAT GGAGGGCAGC 
AGTTTCCATC GCGAAGACGC GGAGATCCAG CGCATGAATG ATCTGAGCTT AGTGCCCAAA 
TCTACGGGGA ATGTTTCCAT CGGAGCATAT GGACAATTTT ACCATCCGCT CTACTTCCAA 
CTGCCTCCGC AGTTCTACGG AGATCGGACT AGTAGCTATG GTGGCTTTTT GTACTTCACC 
CTGATCACCG AAGGTGCCCA CAAGCCCTTG GAGCGAAATA TTTTGGGTCA GTATCCATTG 
GTTCAGTTAC ACGCGCACTC CAAGTTGCTA CTGGACTTTT ACGAGTACGA AGAGTTCGAG 
TACTCCTTGA ACGTGACCCA TCGGGTGCCG CTGCATGAGT CCTTCTGGAA GTACCACCAC 
ACCAGCCAGG CAGTGGATCG TAATACTCTA ATGGCTGCCC TCCAAAACAT TCGACACATC 
TTCATTCGAG CCTTTGCTTT TGCCGATTTC CAGGAAGTTG TCCTTCAAAA TGTACACATG 
GATGCGGCCA TCTATATCAA GGGCTCCACC AATCTTATAG CCAAGGGAGT CGAACGGTGT 
AAGTGTCCCA AGCGGTTTGA TGGACTTTCA TGCCAGGATC CGGGAAGATC CTTCTACCGT 
TGGCGGAATA CCACTATTGT GGAGAGTGTA TTCATTGAGG ATCTGATAGG AAGAGCAGCT 
CCCTGTCATT GTAATGGTCG TAGCAGCGAT TGTGATCGCG AAACTGGAGT CTGTCAGAAC 
TGTCGAGGTA ACACTGGAGG AGATCACTGC CATCAGTGTG CTGAGGGATT CTACGGCGAT 
CCCAATTCTC CACACGGTTG TCAGGCTTGT CCGTGCCCGG AGACCAATAG GAACTTTGCC 
CGGGGCTGCA ATGTCTGGGA CGGAGAAGTC AGTTGTGTCT GCAAGCCGGG TTACACGGGC 
AGATTGTGTG AGCGGTGCCA GGCGGGATAT TTTGGAGATC CTATGAGATA TCCAAACACT 
ACTTGCCAAC CTTGCAACTG TCACCCAGAT GGAATCCAAA CCGAAGGCTG CGATGTAGAG 
ACTGGTAGAT GCTACTGTCG AGAAGGAGTT ACTGGGCTAA AGTGCGACAA GTGTCAGGCG 
GAACGTCATC ATCTGGTGGA CAACGGATGC AAAATCTGTG ACAATTGCAC TCTTCTGCTT 
CTGGATTATA TGGAGCTGGT GGGCAACAAG CTTCGTCGTG GAATGCACAA CATGGATTTG 
ACTGGAATTC CGGCGCCATA TCGAAAATTG TCAGAGTACG AAAGCGCCTA TGAGAAATGG 
AATGGGCGCC ACTGGGACTT TAGCCAGACC AAACGGCGTC TACAGGATTA CGACTCCGCT 
GACATCCTAA AGCTGGAGGC CCACGCCGAA AATTTGAAGT TTCAATCCCG CAAAGCAGTG 
GCCACCATTG GAAAACGGGA GTTTGCCATA AAATCGATGC GGGAAGATGC TGTAACCCAA 
CAGCATAGTG TCGGCTTACT CCGCTCGGAG ATTCTTCAAA CCCTTTCCGA TTTACATGGC 
TATGGAAAGA GTGCCCATTA TCTCAGTCTG CCTACTGCTC TCAAGCAGGC CCGATTCTAC 
CTGCAGGCCA TCCGGGAGCA CGACCAAATG GTTCAGGGAA TCCGCAGTAC CAACGACTGT 
GCATGGAAGC ATTTTTATGC CATGGGCAAC GCATCGGATG CGTCGTTCGA CGAGAGCGGC 
CGCTTGGAGA TGCTCTGGCG GGACTTGAAT CAGACCAATC ATCGAGTGGT AGACATGCGG 
CTGCAGGTGG ATCGCGTGCA AGAGGTGGAG AACGAAGCGG AGGACGTTCT GGAACATGTG 
CGCAATTTGA GTATTCGCGT GGGTGAATCC CACCAGGAGC TGGATGAACT TAACCAGCGT 
ATTTCCGATC ACCTAGATCC GGGCTACCTA GAACAGGGCG AGGGCCTGCT AAGGCTGACA 
GTCCAGCGGC AGATAATGCT CAATGGGCAT CTTAACCAAT TGGATGGCTA CCGTATACTA 
CTAAATACCA CATTGGGCGT AAAGACTGAG CAGCAGCGGG AGGTGCGTAA ACACTGGCTT 
CCCAAGGCGG AAAAGCACGC CAGTCATCTG CTGGCGCGAT CCAATGAGTA CGCCCGCAAG 
TTTCAACCCA CCAGAAATGG AGCCCGCATA GCCATGCTGG CCAGTTCGGC CCACAGCAAT 
ATCACTGAGG CCATCAACGA TGCCCGGCTA GCATCAATCC TGGCCAAGGA ACGGGTGTAC 
GAGGCGCAGA GAACACTCTA TCCCAGTGAC GGCAGCTCCA TGATCGAGCG AGCCAAGCAC 
TCGCTGCACA GATCAAAGCA ACTTCAGCAA GAAGCGCTCA AGCAGATGCA CAAATCCAAT 
GTGTTGAAGG ACAAACTGCA TCGCCAGGAG CAGCAGGTGG AGGGCATCAA GTCCACGATT 
TACGATTCCG GACTGCGCAC CAACAACATA TCCGGACAGC TGCAGGGTCT TTCAGATGGT 
TCAGCACGTC GTCAAGCGAA GGATAGTCTG GAAATGGCTG ATAGAACGGG AGAACAAATG 
CGTGCTGAAC TGCAAAAGGC GAAGGATATG CAGAAGTCCA TCCAAAACAT GCGAAATTCG 
TTCTCCAATC TGGAACCCGA CTGGGAAATC AAGCTGGGAA TGGCCCAAGA GAACATATCC 
CTGACCCAGA CTAACTTGCG TCTGGCCAAT GTCTCATTGA GCTACCTAGA GCAGCAGGCC 
GAGAAGGAGC AACAGGTGTT CGAGGTGTGG AACAACAGCA TGGCCCAGCA ACTGCAGCAG 
CTCCGGGATC AAATAGCCAA GGCCAGACAT GCAGCAGAAG CGATCGATGT TTCCCTGGAA 
TCGCTGGGTC CTAAATGCAT TCGATCCTAT TTGCCCGCCT CCTACGGTTT GAGTACCTCG 
AATAAGATTC GTATGAGCTT CGCCTTATCC AATCACCTCG AGAGCTCTCC ATTGATTCAC 
CTGGCCAGCA GCGAGGGGCG TCACATCACC CTGGAGCTGT ACAAACGTCG TGTGCGCCTG 
GTGTGGAATC TTGGTGGCAC CACCGCAACC GTTACGCATC CCATGGTGGT GCATACTCGT 
GACCCCAAGT ACGACGATGC CTGGTACCAT GTGGAGGCCA ATAGGACACT TAATCTGGGC 
AGCCTGGTCG TGCGTCGAAT GAACAACTAT GGCGAGCTGA CTCCGCCTAA CCCGGTCATC 
ATAACAGGAT CCACGGATAC GGAGCACACA CGTTTCTATC AGTCTCGAAG CGATCGAATT 
TCGCTGGGAG GCTTTGCATC GAAAGATTTG CAATTCACGC CGGGACTCAA TGTGGTGGTG 
CATCAGGTGG AGGTGGACAA CAAACCGCTG GGTCTGTGGA ACTTCGTGAC CAGCGAGGGC 
AGCTGCGGTG GATCAATGGT TGGAGCTAAG GAGTCTTCGG CCTCGTCCAC GGCTCGCCAT 
TTCAATGGAT TAGGCTATGC CCAGTTGATG AAGACCCGAC CACGACCCAC TCGCAAAAAC 
CTGTTCTCCG TGCAGATGAC TTTCCGCACG TTGGACGAAA ATGCCTTGCT CTTCTTGGCG 
GTTGATGACA AGAACAACCG CTCGGTATCG GTAACTTTGA GCCGGGGTCG GATCATGTTC 
CGCATTGACT ATGGCGATGA GTCCAAGCTG GAAATCAACA CCACTAAGAA GTATAATGTG 
GGCCAGTGGA TCAAGATCGA GGCTGCGCGA GAGTTCTCGG CGAAAAGAAG TACTGAAAAT 
GGAATGCTCC GAGTAAACAA CGATCGTCCA ATTTCCGGAG CACCTACACT ACCCGTAAAC 
ATTCACTTGC TTCCGGATCT TTCCAAGACT GTCTATTATC TGGGCGGTGT GCCACCAGGT 
TTCACTTCGG GAACCTCAAA GGCGCCTGGT GCTGATAATC CCTTCTTGGG ATGTATGAAG 
GATGTCCAGG TAAATGGCGA GACCTACGAT CCTCTGGAGA GCTCAAGCTA CTACGGTGTG 
GAGCCTTCCT GCAAGAACAT GATTACCAAG GCTGGTTTCT CGGGCAATGG TTACTTGGAG 
CTGCCATCGC AGTCACTACG AAAGCGCTCC AATACCGCTT TGGTGTTCCG CACCCTTCAG 
CCCGATTGCC TGCTGCTCCT TGCTGCCTAT CCGCCTGAGA TCCTGGGCGA CTATGATGCT 
AAGGACATTA AGGGCAACTT CTCCATTAGC CTGGTCGATG GTCAATTGCA TGTTTGGGTT 
AACTCTGGTC GCAGCTTCAT CAAGATGTCC TCGAACTCCA GTCAGATGAA CGATGGCGAG 
TTTCACGTGG TACATTTGAT CAAGACCGGT CGCAAGCTGG AGCTGATGGT GGACGATGAG 
CTGCAAGAGA TTCGCAATCT AAACGGCAAT CCCACGGTGG TCAGCCTGCC GAGGGATGCA 
GGTGGCCTGT ATATAGGTGG AGCTCCGCCG CACGAAAGTT ATACGCCATT GGCGCCGACT 
TTTGTAAATC TAGAAGGAGC CATTCGGGAT GTGGTGTTCA ACAATCGGAC CATTAACTTT 
AACGATGCCC TGACCTTTGC CAACGTCCAG ATTGGACGTA ATGGTCCACT GATGGGCAGT 
CTGAAAGGAG GACTGTACGA TGTCCTCCTG AAAACCGAAC CCATGATTGG AAAGAGTTTT 
ACGGCCTCGC CTGAGGGCTG CAAGCGGATC GGCAGCTATT CCTACGAGCC GAATGCATTC 
AAGTTTGGAG ACGATATCTA TAGCTATTCG CAGCTGAAGC TACCGGAGCG TCATTTCTGG 
CAGCGCAACT TCCATCTGAG CTTCGATTTC CGCTCCTTCT ATCCCAATGG AATGCTCTAC 
TTGTCGCCGG GAAGCAAGGA GAAACCCAAG CACTACGTGG CTCTCGTCCT CAAGGACGGA 
CAGCTGGTCC TGGTGGTGCG AGGTCGTCGG CGCGAGGAAC TCCAGTTGAC CGCCAAGTTA 
AACGATGGCG AATGGCACCG GGTGACGATT AGCTGTCACG ATCGCAAGGT CACCATGTCG 
GTGGAAATAG GACGTACAGA TCAAAAGACT TCCGCCCAGA TGAAGCTGCC CAAGAAGATT 
GGCGCCTCTC AGCTTTTGCT GGTTGGTGGG CTACCACAGT CTCCAGTCAA GGTCTCCTCC 
GATCTCTATG TCCGTCTGGA ACCATTCAAG GGTTGTCTGC GCCGGGTGAG CATCAACAAC 
AACACCCAGG ATCTGGCCCG ACCCGGCAAA CATTCGAATG TGGGTCAGTG CTTTCCCACG 
GTGGAGAGAG GCAGTTACTT TCCCGGCGAT GCCTATGCCA TTTACAAGAA AAACTTCAAT 
GTGGGCAAGT ATCTCGATCT GGAAACGGAG TTTAGGACCT CGGAGCTATC GGGCATTCTG 
CTGAGTGTCT CTGATCCAAA TGGATTCCCA GCGCTTTCAC TGGAACTGCA TAATGGCAAT 
ATAATATTCT CTTGCGATCC TGGAAACGGA GCGCCCATGC GTGTGGAGTC ATCACTGCCC 
ACCAAATATG CCCTATGTGA CAACAAATGG CATAATATTT CAGCTCTCTA CGATGGCGAA 
CAGATTGTCC TGCGCATCGA TCAATTGCCA GCCGTGATCA GTGTGGGTAA TCAGGGGAAT 
GCTGGAAAGG TGCAAACCCG ATCCCCGCTC TATATTGGCG GTTTGCCAGA GAGTGCTCCC 
AGTGGTTCAC TGATATCGCG GGAGAACTTC AAGGGCTGCA TCCGGCACGT GTCCATACGG 
AACGAGAGAC GCGACTGGAT CGAGATGGAG GACCTGAGGA ACGTGCTGCT CAGCGAGTGC 
CTCGTGTCCA GCGATGAGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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