axo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	axo
Entrez Gene ID	43923
Full Name	axotactin
Synonyms	CG13715,CG13717,CG18296,CG32234,CG42664,CG43225,CT1277,Dmel\CG43225,Dmel_CG13715,Dmel_CG13717,Dmel_CG18296,Dmel_CG32234,Dmel_CG42664
General protein information	Preferred Names axotactin Names CG43225 gene product from transcript CG43225-RG CG43225-PB CG43225-PC CG43225-PD CG43225-PF CG43225-PG CG43225-PH CG43225-PI axo-PB axo-PC axo-PD axo-PF axo-PG axo-PH axo-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64B12-64B13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274494.1	NP_001261423.1	axotactin, isoform G
NM_001259703.1	NP_001246632.1	axotactin, isoform D
NM_001274493.1	NP_001261422.1	axotactin, isoform F
NM_001259701.1	NP_001246630.1	axotactin, isoform B
NM_001274495.1	NP_001261424.1	axotactin, isoform H
NM_079917.5	NP_524656.5	axotactin, isoform I
NM_001259702.1	NP_001246631.1	axotactin, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006513	XP_316540.4
Drosophila melanogaster (fruit fly)	axo	NP_001246630.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following axo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the axo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa21256	NM_001259702.1 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
OFa21256	NM_001274494.1 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
OFa19338	NM_079917.5 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa21257	NM_001274493.1 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa21258	NM_001259701.1 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa21259	NM_001274495.1 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa21259	NM_001259703.1 Latest version!	Drosophila melanogaster axotactin (axo), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

axo ( NM_001274494.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261423.1 Drosophila melanogaster axotactin (axo), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa21256
Clone ID Related Accession (Same CDS sequence)	NM_001274494.1 , NM_001259702.1
Accession Version	NM_001274494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCATCCCA TCCAACCTCC TGATGCCATT TTCATGCCCA ATCTCCCCCA GAAAGCGCAG 
CAGCGGCAGC CGCAGGAACA CAAATCCCGC CACAAAGCGA CCGATGACAC AGAGGCTCCA 
AAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGTCCTTTG ATGTAGCCAC AAATGCCAAT 
GGGTCCTCTC TGCCAGCGAG TCGGGTGAAA AATCCGGAGG AGCCGGGAGG AAAATCCCCA 
TTGGTGCCGA TGCGACAGCA TCACTCGAAA GTGACGCGCC CACCCCCGCC GCCGACGCTT 
TTCCTGGAAA ACTCACTGCT GCAGCGCCAG TTCGCGAATC CAATTAGTTA CTTGGGTGGA 
CCGCGTCTGC AGCCGCGCAG TAATCGCACC AGCATCGACT CGATATTATC GCTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261423.1
CDS	394..6933
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPHPIQPPDAIFMPNLPQKAQQRQPQEHKSRHKATDDTEAPKQQQQQQQQQQSFDVATNANGSSLPASRVKNPEEPGGKSPLVPMRQHHSKVTRPPPPPTLFLENSLLQRQFANPISYLGGPRLQPRSNRTSIDSILSLD

Target ORF information:

RefSeq Version	NM_001274494.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274494.1

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCATCCCA TCCAACCTCC TGATGCCATT TTCATGCCCA ATCTCCCCCA GAAAGCGCAG 
CAGCGGCAGC CGCAGGAACA CAAATCCCGC CACAAAGCGA CCGATGACAC AGAGGCTCCA 
AAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGTCCTTTG ATGTAGCCAC AAATGCCAAT 
GGGTCCTCTC TGCCAGCGAG TCGGGTGAAA AATCCGGAGG AGCCGGGAGG AAAATCCCCA 
TTGGTGCCGA TGCGACAGCA TCACTCGAAA GTGACGCGCC CACCCCCGCC GCCGACGCTT 
TTCCTGGAAA ACTCACTGCT GCAGCGCCAG TTCGCGAATC CAATTAGTTA CTTGGGTGGA 
CCGCGTCTGC AGCCGCGCAG TAATCGCACC AGCATCGACT CGATATTATC GCTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

axo ( NM_001259702.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246631.1 Drosophila melanogaster axotactin (axo), transcript variant C, mRNA.

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CloneID	OFa21256
Clone ID Related Accession (Same CDS sequence)	NM_001274494.1 , NM_001259702.1
Accession Version	NM_001259702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCATCCCA TCCAACCTCC TGATGCCATT TTCATGCCCA ATCTCCCCCA GAAAGCGCAG 
CAGCGGCAGC CGCAGGAACA CAAATCCCGC CACAAAGCGA CCGATGACAC AGAGGCTCCA 
AAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGTCCTTTG ATGTAGCCAC AAATGCCAAT 
GGGTCCTCTC TGCCAGCGAG TCGGGTGAAA AATCCGGAGG AGCCGGGAGG AAAATCCCCA 
TTGGTGCCGA TGCGACAGCA TCACTCGAAA GTGACGCGCC CACCCCCGCC GCCGACGCTT 
TTCCTGGAAA ACTCACTGCT GCAGCGCCAG TTCGCGAATC CAATTAGTTA CTTGGGTGGA 
CCGCGTCTGC AGCCGCGCAG TAATCGCACC AGCATCGACT CGATATTATC GCTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246631.1
CDS	285..6824
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPHPIQPPDAIFMPNLPQKAQQRQPQEHKSRHKATDDTEAPKQQQQQQQQQQSFDVATNANGSSLPASRVKNPEEPGGKSPLVPMRQHHSKVTRPPPPPTLFLENSLLQRQFANPISYLGGPRLQPRSNRTSIDSILSLD

Target ORF information:

RefSeq Version	NM_001259702.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259702.1

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCATCCCA TCCAACCTCC TGATGCCATT TTCATGCCCA ATCTCCCCCA GAAAGCGCAG 
CAGCGGCAGC CGCAGGAACA CAAATCCCGC CACAAAGCGA CCGATGACAC AGAGGCTCCA 
AAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGTCCTTTG ATGTAGCCAC AAATGCCAAT 
GGGTCCTCTC TGCCAGCGAG TCGGGTGAAA AATCCGGAGG AGCCGGGAGG AAAATCCCCA 
TTGGTGCCGA TGCGACAGCA TCACTCGAAA GTGACGCGCC CACCCCCGCC GCCGACGCTT 
TTCCTGGAAA ACTCACTGCT GCAGCGCCAG TTCGCGAATC CAATTAGTTA CTTGGGTGGA 
CCGCGTCTGC AGCCGCGCAG TAATCGCACC AGCATCGACT CGATATTATC GCTGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

axo ( NM_079917.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524656.5 Drosophila melanogaster axotactin (axo), transcript variant I, mRNA.

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CloneID	OFa19338
Clone ID Related Accession (Same CDS sequence)	NM_079917.5
Accession Version	NM_079917.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079917.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCCAAATA GAATTAAACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524656.5
CDS	394..6537
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPPNRIKP

Target ORF information:

RefSeq Version	NM_079917.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079917.5

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCCAAATA GAATTAAACC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

axo ( NM_001274493.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261422.1 Drosophila melanogaster axotactin (axo), transcript variant F, mRNA.

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CloneID	OFa21257
Clone ID Related Accession (Same CDS sequence)	NM_001274493.1
Accession Version	NM_001274493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCAGCATG AAATGCAAAA GCAGCTGCCG CCGCAACGAC AACTGTCTCA ACGTGGAGTT 
GGAATCCAGC AGCAGACGAG CTCCAAGCGA AGGAAGCCAG TAGGAAGTGG TGGAGCCGGA 
GGTGCAACTG TAGTTGGACC GGCCACAGTG CGGCGACAAG TTTCTTGGGG GCCGCCAATC 
GTGGCCGAAA CGGATGTGGA TTTGGCCAAC AACAGCAGCG GCAATGGCAA CGGCAATGGG 
AATGGAAATG GAAATCGCAA AGCGGGCGGC AAACAAAATG GATACCATGG CGTCAATAGA 
CGGGCCACCA CCACCAGGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261422.1
CDS	394..6837
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPQHEMQKQLPPQRQLSQRGVGIQQQTSSKRRKPVGSGGAGGATVVGPATVRRQVSWGPPIVAETDVDLANNSSGNGNGNGNGNGNRKAGGKQNGYHGVNRRATTTRN

Target ORF information:

RefSeq Version	NM_001274493.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274493.1

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCAGCATG AAATGCAAAA GCAGCTGCCG CCGCAACGAC AACTGTCTCA ACGTGGAGTT 
GGAATCCAGC AGCAGACGAG CTCCAAGCGA AGGAAGCCAG TAGGAAGTGG TGGAGCCGGA 
GGTGCAACTG TAGTTGGACC GGCCACAGTG CGGCGACAAG TTTCTTGGGG GCCGCCAATC 
GTGGCCGAAA CGGATGTGGA TTTGGCCAAC AACAGCAGCG GCAATGGCAA CGGCAATGGG 
AATGGAAATG GAAATCGCAA AGCGGGCGGC AAACAAAATG GATACCATGG CGTCAATAGA 
CGGGCCACCA CCACCAGGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

axo ( NM_001259701.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246630.1 Drosophila melanogaster axotactin (axo), transcript variant B, mRNA.

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CloneID	OFa21258
Clone ID Related Accession (Same CDS sequence)	NM_001259701.1
Accession Version	NM_001259701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCAGCAGC ATGAAATGCA AAAGCAGCTG CCGCCGCAAC GACAACTGTC TCAACGTGGA 
GTTGGAATCC AGCAGCAGAC GAGCTCCAAG CGAAGGAAGC CAGTAGGAAG TGGTGGAGCC 
GGAGGTGCAA CTGTAGTTGG ACCGGCCACA GTGCGGCGAC AAGTTTCTTG GGGGCCGCCA 
ATCGTGGCCG AAACGGATGT GGATTTGGCC AACAACAGCA GCGGCAATGG CAACGGCAAT 
GGGAATGGAA ATGGAAATCG CAAAGCGGGC GGCAAACAAA ATGGATACCA TGGCGTCAAT 
AGACGGGCCA CCACCACCAG GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246630.1
CDS	285..6731
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPQQHEMQKQLPPQRQLSQRGVGIQQQTSSKRRKPVGSGGAGGATVVGPATVRRQVSWGPPIVAETDVDLANNSSGNGNGNGNGNGNRKAGGKQNGYHGVNRRATTTRN

Target ORF information:

RefSeq Version	NM_001259701.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259701.1

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGCAGCAGC ATGAAATGCA AAAGCAGCTG CCGCCGCAAC GACAACTGTC TCAACGTGGA 
GTTGGAATCC AGCAGCAGAC GAGCTCCAAG CGAAGGAAGC CAGTAGGAAG TGGTGGAGCC 
GGAGGTGCAA CTGTAGTTGG ACCGGCCACA GTGCGGCGAC AAGTTTCTTG GGGGCCGCCA 
ATCGTGGCCG AAACGGATGT GGATTTGGCC AACAACAGCA GCGGCAATGG CAACGGCAAT 
GGGAATGGAA ATGGAAATCG CAAAGCGGGC GGCAAACAAA ATGGATACCA TGGCGTCAAT 
AGACGGGCCA CCACCACCAG GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

axo ( NM_001259703.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246632.1 Drosophila melanogaster axotactin (axo), transcript variant D, mRNA.

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CloneID	OFa21259
Clone ID Related Accession (Same CDS sequence)	NM_001259703.1 , NM_001274495.1
Accession Version	NM_001259703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGGTAAGCA GAGGATCCCA ACCTACAGTA AACCTTGTCA GAATGGCGCC AATAGCCACA 
ATCAGCGAAA CCAATTTGCA TGGATTGGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246632.1
CDS	285..6497
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPVSRGSQPTVNLVRMAPIATISETNLHGLG

Target ORF information:

RefSeq Version	NM_001259703.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259703.1

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGGTAAGCA GAGGATCCCA ACCTACAGTA AACCTTGTCA GAATGGCGCC AATAGCCACA 
ATCAGCGAAA CCAATTTGCA TGGATTGGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

axo ( NM_001274495.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261424.1 Drosophila melanogaster axotactin (axo), transcript variant H, mRNA.

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CloneID	OFa21259
Clone ID Related Accession (Same CDS sequence)	NM_001259703.1 , NM_001274495.1
Accession Version	NM_001274495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axotactin, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGGTAAGCA GAGGATCCCA ACCTACAGTA AACCTTGTCA GAATGGCGCC AATAGCCACA 
ATCAGCGAAA CCAATTTGCA TGGATTGGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261424.1
CDS	394..6606
Translation	MAFPYIWALLPLICSASGLSLPNMTSTDAVVAGGGILPILVAGNPGNLGSSNMSLSGGGGLAGSSTGGQSLPDTGGGNSAGGSPAGGSSGTGGGGSNSGISGNNSAMIQGQKSNQYEKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFLQSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIFAKNNTFIQMDGDIIQTFQLRLCREISFQFRTRLPHGLLVYHNVKNPDRINLDPYALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVMVRIDVHGARLIARVDNSQEEVYLKGLNHEYNYGVSTNLPSVVLVGGLSSEEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGSRCINHYGGISCDCFGTHYEGEHCDIYTATIITLRGASYVSYRIYDWKDRVHSSTRRISLMFRTNFDDSALFYASGESLKHQYIAASIKNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILHEQRTVSIILDQQQKVLELPATASGNMLFDPEIYFGGGPELHKKKGLASHNNFVGSLKYVYYNDISILYELQRGNPKVHYHGVLEAEFVENEVNVIPITYPFATSHIWWPINHAEEFNIKFDFRSSRPGAVLAYSDVTTSAGNGFWEIRLTSDKLSFDLVPDVNNNVTHSTTIKINRATSWHSVELDYKLGEIRFTVDYRHTLSQMYGLTFNIGDKLIIGSSLKSAAMGLVGCIRDIEINGHLIEPRHVVKTERVVGEVALDNCNYIDPCKRPNTCEHGGKCFVKDDRVTCDCKHTGYIGKNCHFTKYRKTCEELALLGFTKSDVYLIDIDGNGVFPPAHVKCDFQSLENATKTIVEHNLPSQVDVRSARESDFSFNIRYREFSPHMLQELISHSLYCTQYIKYDCYRAQLELHSATWFTSSAKNLTVDFLGNVKRGACPCSVNKTCVDPNQSCNCDVKENKWNSDEGYYQDPQSLGITNMYFLQQKDMDDEAQGRITLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRPHYPSFMVALTGDDQLTFTFTLSTGTTRELVINSHRRLNGGEWHKIWIDYNQYHVRFMINTDYQMLDLLPEEEFGPFEGSMYIGGATFDLLKKLSVKAGLIGCFRGLVVNGEILDIYSYMSVHLSEIIKDCKPSCVPSPCRNGAQCKELWSSFKCVCNNPWAHIGEFCETNINEKALTFINRESFLMRNYLSVGATPVILMHGINGERDVLKGILNQDLLINLRTYDTNALVLYANDHYNNFVHLYISLNREIVFLYNYGDEIVNLTLLDDTLMASLKSIQVAIVRGEQETRMHVNEHSVSIDRGTLLLDEYANKPWSNPEKEVLSPHRPPAPPTEYFQFHVGGYDPANLLRPNVDAPALEGYIGCVRGLKIGAQLIDLADINERNIAPTQEGVLPNCQIKCDAEPCKNGGTCQEHFAEQLSTCDCEHTSFLGEFCSEEKGADFSGESTLQRKFELPGTGRVDYVRLQLAFSSFDLRRANRIMLLMQTEAERSYYLLLAITSDGYLQLEEDRDNGQTVGARIDRNFLNSARHSVYYVRNGTQSQLFIDREQVPLSEFAARVLTTGGDAGSNRVQIGGINSTDSRFAVFKSYSGCLSNIYIQVNGHVMKPLEEYMLFTKSGADNITVINPQGVRSAQCNAKFDVSEQPTQEPMVNVSMIPEPWVEEPPARVPYIPRFVYDENKQEDSTQVVFLTLTSVFVIIVICCLLEVYRSHLAYKKRIERETDEDIIWSKEQATKMHESPGVKAGLLGGVTAGSGNGLPPYTYKALPQEDKKPGNGAPLVGILKNGSATPSQPGTPTALSKNGDIASRIEEEEEEEDEAPAQKAAEKSGENEEPPAKDTTASEIKESQAQPPEQLAKDTTDASAAPKASKETEAQAEPSEPSSQLNSAQNGQLAQMEQAARGDEVQVPSLPVSRGSQPTVNLVRMAPIATISETNLHGLG

Target ORF information:

RefSeq Version	NM_001274495.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axotactin (axo), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274495.1

ORF Insert Sequence:

ATGGCATTCC CTTATATCTG GGCACTACTG CCCCTCATCT GCTCCGCTTC AGGTCTCAGC 
CTCCCAAACA TGACCTCAAC GGATGCAGTG GTTGCCGGTG GAGGCATTTT GCCAATCCTG 
GTGGCAGGAA ATCCCGGCAA TCTGGGGAGC AGCAATATGA GCCTGAGCGG CGGGGGAGGA 
TTGGCCGGCA GCAGTACCGG CGGACAATCG TTACCGGATA CGGGAGGAGG TAACTCTGCG 
GGCGGCTCGC CGGCGGGTGG CAGCAGCGGT ACCGGCGGCG GTGGCAGCAA CAGCGGCATC 
AGTGGCAACA ACTCGGCCAT GATCCAAGGA CAAAAGAGCA ACCAGTACGA GAAGTGTGCG 
GGTCCTGGAG ATCCGGGTCC ATGCAAGCAG TACATCTACA AGTGGCGCTA TGAGCCAACC 
ACCAACGAGT GCACCAACTT CATTTGGGGC GGTTGTGAAG GAAATCCGCA GAACCGTTTC 
GGCACCGAGG CGGAGTGCCT GTTCCACTGC ATCGGCGGAC CACACACTCT GCCACCGTTC 
CTACAATCCA CCACCCGTGA GCCAAGCACT ACGGAGAGCT CCATGCTTCT GGGTCTGCCG 
TACACCCAGA GTCCGGCTCA GTCACCGGAC GGAATGGGCG GGGCAGAGGG CGGTGATGGC 
ACAACGCCCG TGCCCATCGA ACAGCGCGGA CCCGAGCTGA CTTTCGCGGA GACGGGCCAA 
GGCAAAACTT TCATTTTCGC CAAGAACAAC ACGTTCATCC AAATGGACGG CGACATTATA 
CAGACATTTC AATTAAGACT TTGCCGTGAG ATTTCATTTC AATTCCGCAC CCGACTGCCG 
CACGGCCTGC TCGTCTATCA CAACGTTAAG AATCCGGATC GCATTAATTT GGACCCCTAT 
GCACTCTACG TGATTGTGGA GAAGGGCCAG CTGAAGGTGG TCCATGTCTT TGGCAAGCAT 
TCAACGAGTG TCACTGTTGG CGAGAGTCTA AATCGAGATG AATGGCACAG TGTGATGGTG 
CGCATCGATG TCCATGGTGC AAGATTAATT GCACGCGTCG ACAATAGTCA GGAGGAGGTC 
TATCTGAAAG GACTGAATCA CGAGTATAAC TACGGCGTCT CCACGAATCT GCCATCGGTG 
GTCCTGGTCG GAGGCCTTTC CTCGGAGGAG AAGCTGCACG GCGTGAAATA CATTACTGAG 
TCCTTTGTGG GCTGCATTCG CAATGTGGTC CTGAGCTCCG GCAAGGCCGC CTCCGACCTC 
CTGCCCATCG CACCCCTGGT GGCCACCAAG CACGAGAACG TCAACGAGGG ATGCTCGGAC 
ATGTGCGAAT CCCGGCACAA CCTCTGCTTC GTGGGATCGC GCTGCATCAA CCACTACGGA 
GGAATCTCCT GCGATTGCTT TGGCACCCAC TACGAGGGCG AGCACTGCGA CATTTACACC 
GCCACCATAA TCACATTGCG AGGAGCTAGC TATGTATCGT ACAGGATATA CGACTGGAAG 
GATCGAGTGC ACTCCTCCAC CAGGCGTATT AGTCTCATGT TCCGTACGAA TTTCGACGAT 
TCAGCTCTAT TCTACGCCAG TGGCGAATCA TTGAAACATC AGTATATAGC GGCTTCCATT 
AAAAACCAGT CGGTTCACGT GGAAATGGAC TTTGGCGACA ATGTGATGAG CACCGTGCTA 
ACCGACGACC TGACCCGCGG TTATTGGCAC AATCTGACAA TACTACACGA ACAGCGAACG 
GTTAGCATTA TCCTGGATCA GCAGCAAAAG GTCCTGGAAC TGCCAGCAAC GGCCAGTGGG 
AATATGCTCT TCGATCCGGA AATATATTTC GGCGGAGGAC CGGAATTGCA TAAGAAGAAG 
GGTCTGGCCT CACATAACAA TTTCGTGGGC AGCCTGAAGT ATGTCTACTA CAATGACATA 
TCCATTTTGT ACGAACTGCA GCGTGGAAAT CCCAAGGTGC ACTATCACGG CGTGCTGGAG 
GCGGAGTTTG TCGAGAACGA AGTGAATGTC ATACCAATCA CCTATCCCTT TGCGACATCA 
CACATTTGGT GGCCCATAAA TCACGCCGAG GAATTTAATA TTAAATTCGA TTTCCGCAGC 
AGTCGGCCGG GCGCGGTGCT GGCCTACAGT GACGTAACGA CCAGTGCCGG AAATGGCTTC 
TGGGAGATAC GTCTGACGTC GGACAAACTA AGTTTCGATC TGGTACCTGA TGTTAACAAC 
AATGTAACCC ACTCGACCAC CATTAAAATC AATAGGGCCA CCTCCTGGCA TAGCGTGGAG 
CTGGATTATA AGCTCGGCGA GATTCGCTTC ACTGTGGACT ATCGGCACAC TCTTAGCCAG 
ATGTACGGCT TAACCTTTAA CATCGGCGAC AAACTGATCA TTGGCAGCAG CTTAAAGTCG 
GCAGCCATGG GCTTAGTGGG CTGTATACGG GATATAGAAA TTAATGGACA TTTAATCGAA 
CCGCGACATG TGGTCAAAAC TGAACGAGTT GTGGGAGAGG TCGCCCTGGA CAACTGCAAC 
TACATAGATC CGTGCAAAAG ACCCAATACC TGTGAGCATG GTGGCAAATG CTTTGTAAAG 
GACGATCGAG TCACCTGTGA TTGCAAACAC ACGGGTTATA TAGGTAAAAA TTGTCACTTT 
ACCAAGTACA GAAAGACCTG CGAAGAGCTG GCACTCTTGG GCTTCACCAA ATCAGATGTC 
TATCTCATCG ATATCGATGG CAATGGTGTT TTTCCACCTG CCCATGTCAA GTGCGATTTC 
CAGAGCCTTG AGAATGCCAC CAAAACGATT GTGGAGCACA ATCTGCCCAG TCAAGTGGAT 
GTGCGATCCG CCAGGGAATC GGACTTCAGC TTCAACATCA GATATAGGGA ATTTTCACCC 
CACATGCTGC AGGAACTTAT CTCACATTCA CTGTACTGCA CTCAGTACAT CAAGTACGAC 
TGCTACCGGG CTCAGTTGGA ACTGCACTCG GCCACATGGT TCACATCCTC GGCCAAGAAT 
CTCACCGTCG ACTTCCTGGG CAATGTCAAG AGAGGCGCCT GCCCGTGCTC CGTTAACAAG 
ACGTGCGTGG ATCCGAATCA ATCATGCAAC TGCGATGTAA AGGAGAACAA ATGGAATTCG 
GATGAGGGCT ACTACCAGGA TCCCCAGAGC CTGGGCATTA CAAACATGTA CTTCCTGCAG 
CAAAAGGATA TGGATGACGA GGCCCAGGGT CGCATCACTC TGGGTCCGCT GGAGTGTGTT 
GAAACAAATA CGCAAAAGTA CGTGGTCACC TTCACTACAT CGCAGTCGTA CATCGAGGTG 
CCCGGCTGGA GGAAGGGCGA CATTGCCTTC TCGTTCCGCA CCACCGGCGA GAAGGCCATC 
CTGCTATTCC AGCCACCCAT CCGGCCGCAC TATCCATCGT TCATGGTGGC GCTGACCGGC 
GATGACCAGC TCACGTTCAC CTTCACCCTG AGCACGGGTA CCACCCGCGA GCTGGTCATT 
AACTCGCACC GACGGTTGAA CGGCGGCGAA TGGCATAAAA TATGGATCGA CTACAACCAG 
TATCATGTGC GCTTCATGAT AAACACCGAT TACCAGATGC TCGATTTGCT GCCAGAGGAG 
GAGTTCGGAC CGTTCGAGGG CAGCATGTAC ATCGGTGGCG CCACATTCGA CCTGCTGAAG 
AAGCTGTCAG TTAAGGCCGG CCTTATTGGC TGCTTCCGTG GGTTGGTGGT CAACGGCGAG 
ATTCTCGACA TCTACAGCTA CATGTCGGTG CATCTGTCGG AGATTATCAA GGACTGCAAG 
CCATCCTGCG TCCCATCGCC CTGTCGCAAT GGCGCCCAGT GCAAGGAGCT CTGGAGCAGC 
TTTAAGTGCG TCTGCAACAA TCCCTGGGCA CATATCGGCG AATTCTGCGA GACAAACATC 
AACGAAAAGG CTCTTACCTT CATCAACCGT GAATCCTTTC TGATGCGAAA TTATCTGAGT 
GTGGGTGCCA CACCTGTTAT CCTAATGCAC GGTATAAATG GCGAACGGGA TGTCCTAAAG 
GGAATCCTTA ACCAAGATCT ACTGATCAAT TTACGAACTT ACGACACGAA TGCCCTGGTG 
CTTTATGCCA ACGATCATTA TAATAACTTT GTGCACCTCT ACATTAGTCT GAACCGGGAG 
ATTGTTTTTC TTTACAATTA CGGTGATGAA ATTGTAAATC TTACGCTGCT AGATGACACC 
TTAATGGCGT CTTTAAAAAG CATTCAAGTG GCCATCGTGC GAGGAGAGCA GGAAACTAGG 
ATGCATGTGA ACGAGCACAG TGTGAGCATC GATAGGGGAA CTCTGCTCCT TGATGAGTAT 
GCCAACAAGC CTTGGAGTAA TCCGGAAAAA GAGGTCCTGT CGCCCCACCG CCCGCCTGCC 
CCGCCCACCG AGTACTTCCA GTTCCACGTG GGCGGCTATG ATCCGGCGAA TTTGTTGCGC 
CCCAATGTGG ATGCACCGGC TCTGGAAGGC TACATCGGGT GTGTGCGGGG CCTGAAAATC 
GGAGCGCAGT TGATCGACTT GGCGGATATC AATGAAAGGA ATATAGCGCC CACCCAGGAA 
GGTGTCCTGC CCAACTGCCA GATCAAGTGT GATGCAGAGC CGTGCAAAAA TGGCGGCACC 
TGCCAGGAGC ACTTTGCTGA GCAACTCTCG ACATGCGATT GCGAGCACAC GAGTTTTCTG 
GGCGAATTCT GTTCGGAGGA GAAAGGAGCA GATTTCAGTG GCGAATCGAC GTTGCAGCGC 
AAATTTGAAC TGCCTGGTAC GGGTCGCGTT GACTATGTGC GCCTGCAACT GGCCTTTTCG 
TCCTTCGACC TGAGACGAGC CAATCGGATA ATGCTGCTGA TGCAAACGGA GGCTGAGCGC 
AGCTACTACC TTCTATTGGC CATCACCTCC GACGGTTACC TGCAGCTGGA GGAGGACCGA 
GATAATGGGC AAACGGTGGG CGCCCGAATT GATCGGAATT TCCTGAACAG TGCCAGGCAC 
TCGGTGTATT ATGTCAGAAA TGGCACCCAA TCGCAGCTAT TCATTGATCG CGAGCAGGTG 
CCGCTGAGCG AGTTCGCCGC ACGTGTCCTT ACCACCGGTG GCGATGCTGG CTCGAATCGC 
GTCCAGATCG GTGGCATCAA CAGCACGGAC TCGCGGTTTG CCGTCTTTAA AAGCTACAGC 
GGTTGCCTGT CGAACATCTA TATACAGGTG AACGGGCATG TTATGAAGCC ACTCGAGGAG 
TACATGCTAT TCACAAAGTC CGGTGCCGAC AACATAACGG TCATCAATCC GCAGGGCGTC 
CGGAGCGCCC AGTGCAATGC CAAATTCGAT GTGAGCGAAC AGCCCACCCA GGAGCCCATG 
GTTAATGTCA GCATGATTCC CGAGCCATGG GTGGAGGAGC CGCCAGCCCG CGTTCCCTAC 
ATACCGAGAT TCGTGTACGA TGAGAACAAG CAGGAGGACT CCACGCAGGT CGTCTTCCTC 
ACGCTGACCA GTGTCTTCGT CATCATTGTG ATCTGCTGCC TGCTGGAGGT GTATCGCAGC 
CACTTGGCCT ACAAGAAACG CATCGAACGC GAGACGGACG AGGACATCAT TTGGTCAAAG 
GAGCAGGCAA CCAAGATGCA CGAGTCGCCA GGTGTTAAGG CTGGCCTCTT GGGCGGCGTG 
ACTGCGGGAT CGGGGAATGG CCTGCCTCCA TATACCTACA AAGCGTTGCC GCAGGAGGAC 
AAAAAGCCTG GCAACGGTGC CCCCTTGGTG GGCATTTTGA AAAACGGCAG TGCCACGCCC 
TCGCAGCCTG GCACGCCCAC CGCGCTGTCC AAAAATGGAG ACATCGCCTC GCGCATCGAA 
GAGGAGGAAG AGGAGGAGGA TGAGGCGCCC GCGCAAAAGG CAGCGGAAAA AAGCGGCGAA 
AATGAGGAGC CACCGGCGAA GGACACAACC GCCAGCGAAA TAAAGGAGTC GCAGGCTCAG 
CCACCTGAAC AGCTGGCAAA GGACACCACC GATGCTTCTG CCGCACCAAA AGCTTCCAAG 
GAGACGGAGG CGCAGGCAGA GCCTTCGGAG CCATCATCTC AATTAAACTC CGCCCAGAAC 
GGACAGTTGG CCCAAATGGA GCAGGCGGCT AGAGGGGATG AGGTGCAGGT GCCAAGTCTA 
CCGGTAAGCA GAGGATCCCA ACCTACAGTA AACCTTGTCA GAATGGCGCC AATAGCCACA 
ATCAGCGAAA CCAATTTGCA TGGATTGGGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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axo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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