Acf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acf
Entrez Gene ID	43751
Full Name	ATP-dependent chromatin assembly factor large subunit
Synonyms	175K protein,ACF,ACF/CHRAC,ACF1,Acf-1,Acf1,CAF,CG1966,CHRAC,CHRAC-175,Dmel\CG1966,acf1,dACF,dCHRAC,p170,p170/p185,p175
General protein information	Preferred Names ATP-dependent chromatin assembly factor large subunit Names CG1966 gene product from transcript CG1966-RA ATP-utilizing chromatin assembly and remodeling factor Acf-PA CG1966-PA chromatin accessibility complex
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100D2-100D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_080486.3	NP_536734.2	ATP-dependent chromatin assembly factor large subunit

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Acf1	NP_536734.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003137	XP_001237499.3
Caenorhabditis elegans (roundworm)	athp-2	NP_494767.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000668	XP_311194.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACF1 has a role in nucleosome remodeling during Drosophila oogenesis
Title: A role for tuned levels of nucleosome remodeler subunit ACF1 during Drosophila oogenesis.

Altering the levels of ACF1 leads to global and variegated deviations from normal chromatin organisation with pleiotropic defects.
Title: Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation.

The chromatin remodeler Acf1 directly represses Wg transcriptional targets.
Title: The chromatin remodelers ISWI and ACF1 directly repress Wingless transcriptional targets.

ACF1 facilitates HP1 binding to chromatin methylated at lysine 9 within the H3 N terminus (H3K9).
Title: HP1 binding to chromatin methylated at H3K9 is enhanced by auxiliary factors.

The following Acf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06804	NM_080486.3 Latest version!	Drosophila melanogaster ATP-dependent chromatin assembly factor large subunit (Acf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acf ( NM_080486.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536734.2 Drosophila melanogaster ATP-dependent chromatin assembly factor large subunit (Acf), mRNA.

Price & Availability↑

CloneID	OFa06804
Clone ID Related Accession (Same CDS sequence)	NM_080486.3
Accession Version	NM_080486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ATP-dependent chromatin assembly factor large subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_080486.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCATTT GCAAGCGGGA AGGATTCGAC CTGAATCAGA AGGAGGGCAA GAACGAAACA 
TTCCACGACA ACGACCAGGT CTTCTGCTGC TACATCACTA AGCGCATTTT TCGCGACTAT 
GAACACTACT TCCGGCACGT GATGGTGATC AACTCAACGG TGTGGCAGTG CGAGGCCACC 
GGCAAGGAGA ACCTCACCTA CGAGGAGGCG GTCAAAAGTG AGCGGGCGGC TCGCAAGAAG 
ATGGAGCAGT TCAAACAGAG CCTTCGGGCA CCCGTCCTTC TGGTTGTGGA GCACGCCCAA 
CAGTCGGCGG TCAACACCTT GAACATGATA GTGGCCAAGT TTCTGCGCAA GCGCTACTTT 
ATCGGCGAGG AGGTGTCGGT GCAAGCTAAG AAGAACGCCA CGTACACGGT GTTGGGTGTC 
AAGTTGGACA AAAATATGCC GGAACCACTC AATGGTATCT ATGAGGACAC GGACAACCTG 
GTCTACAGAC TACGACCAAA CAAGGGCGAT CCCAGTGCAG AGCTTGATCT GCCCTTTAGG 
CAATTACGTC GCTCGCGCAT GGAGTTTAAC CTGGAAAACC TGAGTATGTT TATAAAGAGC 
AATGTATCGC GGGTGGATGG ACTTCTTCGA CCCAAGCCAG AGGCATACAA GCAGTACGTG 
ACGGACCCTG GCGTAAACTT CTCCACAATT TTCATTGGCA AAATGCCACG TTATTCTCCC 
GCTAAGATTA AAAAACCTGA CGGCAAGAAG CAATCCACTC TCAACAAATA TATTGTCGCC 
GGTGAAGCAA CGGCTGCAAA GTCAAAGGCG AAGGCCAAGT CTGATGCTAA ATCTCTGGCA 
GAGGAATTGG AGCGGGTTAA GCGAGAAAAA GAGGCCAAGC TTATTGAGTT GGAAAAACAA 
AAGGCGGAAA AGAAGGCACA GTTGATTGAA CGCGTTGAAA ACGAATGTAA TTTACTGCTT 
CAAAAGACGG ACGACCTGGA GAGAACCGAC CAAAAAGTCC TGCCCCGCTA CAGACAGATC 
GTCACATTGC TGCCTGAGCA CCTCCTGGGT GATGCCTTCA TGATGCGCGA GTTTATGCAC 
ACTTACACAG GATTGCTGTC CGGCATCGAG GTGTTCCGTC AGAACCTCAG TTTCTACGAG 
ATGACACGTG CACTGACTGC TCGTGAGATT GCGGGCCCAC TATCGGACAT CCTCCTCGTT 
CTGCTTGGCA CTGTTTTCGA TCTGCAAAAA GAAGAGGAGG AGGAATGCGC AGTGACCTAC 
CTCGATCGGG CTGCACAAAC TCAGGAGCCC TATTGGAGTA TGGCGCAGGC CGCCAAAAGT 
CACCTTTACG CCAAGAGGCA CTTTTCCTTT AAGGTAAATG AACTACCATT AGATGCACTG 
ACTCTCAGTG AAGTGCTACG ACTGCATCTA CTTGGATCCG GAGCTTTTGT AAATGAAAAG 
GCGGAGCGCT GGAGAGTCAT GTACCGGAAT GGCTACTCGT CAAAAGAAGA CCCTGGTCTG 
GAACTACGTC TGGAACACTC GCACATCCTG CGCATTCTCA AAAATCATTC CGTATATCAG 
CTAAAGTTTA AGGATATAAT GCTCCTGATT CGCTGCCTGA TGTCCCAAAT TATGACATAC 
TCGGGCACGA TTAACTTGAT TGAGGAGCGT ATGGAGCAAA CAGCAAAGGC CAGGCAAGAT 
CTACGAGCTC TAGTTGTCGG GGAAAATAAG AGATTAGCCG CCGTAGAGAT AAACAGGAAA 
AAACTCACGC AAATGCACCA CCTGGAGGTA AATGGCGTTG AGCCTGAAAA GAGGGAAGCG 
TTGGTCGAGA AATTAAAGAA GAGTATTGCC GAGCTACACG CCCAATCGGA TCAGCAACAT 
CGCAAGCACG AGTTGCAGAT GCTTAAGTTG CATTCGCAGC TTTTCAATTT TTTGGTTTAC 
TTGGGCATGG ATAGGTGCTA CAGGAAATAT TACGTTTTGG AGTCTATGCC TGGGATCTTT 
GTCGAGCATT CGCCGGACAG CCTTGACACT TGTTTGGAGC AACCAATAAC AAATAAGTCC 
CAAATAGAAA TTCGTCAGCA GTCTGCATTG CCAAAGAATC GCAAGGACCT AAGGGTATAT 
CTACTCAAAC TCTACGGAGA CGACGAAAAG AAAACTAAGA AAAAGGCCAA GCACTCTCTA 
GAGAACAAGG AGAACCAAGA GCATCGCCTT AACGGCAGTG CAGAACCCAT GGATGTCGAA 
TCTGATTCTC CAGAGGCTCC CACTCATTTT GAGCTCTTGA TGTGCAGTGG TGACAAACGA 
TCTTGTATTG TGCATGATTC AAGAAATGGG CAACGGCAGC GTTGGGCATA TATTTACAAA 
GCCGAGGAGA TCGATGAATT AATTAAGGCT CTCAATCCCA ATGGTCTCCG TGAATATGAA 
CTCCTTCAAG AGCTATCCGT TCTGCGTTCC TTAATCGAAC AGCATGCTAA GACCTGCCCA 
GTAGACTTGC TAAGTCTAGA AAACGAAACA ATGCGCAAGA AGTTTATGGC GGCGATGGAA 
TCTGAAACTA ACCGTAAATA CGGAGAAGCA AACTTTGGGT TACCGAATGG GACCGATCTA 
AACGAAGTGA TGCGCTTGCA TTTAGTGGAT CGAATTATTC AATTTGAAAA TGATATTTAC 
ACTGGTGATC TCGGCCGCCT GAAAGTTAAG GATATGGAAA AGTGGCGAAG TGACTTGCTT 
GGAGGCAATT ACGACGCTCA GTGCAAGTTG CAGTGGGGCC CGGGTGGCAA GTTGGAAGAT 
GAAGCTGGTT CTGACAACGA ATCCCACGAA ACTCACGAGG AGGACGATGG CGCACTATTA 
GGTAAATATG CCAGGAAACC ATATCGCGAT CCTGGCATGT ATTTGGCAGC TTCTGCAGAC 
ACAAAACCCC TACCAGACAG CGACGATGAG GAGGATCAGC ATACGAATGC CGTCTCGATT 
CCTATCGCCG TGCATAACAT GGCCTCGGCC CTTCTGCAAG TGGAGCAGGC TATTGGAAAA 
CGGTTTCTGA AGGAACCCTA CGGCATGAAG AAGTGGGACC CCAAGCAGGA GGCTTTAAAA 
CTTGCCTGCG ACTCGCGTTT GCACCAGTGG GAAGTTTCGC TAATGGAAAG TACAAGCTTT 
GCTCAAGTCT TTCTGCACCT AAACATCTTG CACGACTGTA TTCAGTGGAG GCGTTCCACC 
AATAAGTCAT TATGCAAGGT CTGCAGGCGT GGCAGCGATC CGGAGAAGAT GCTGCTGTGC 
GATGAATGCA ACGCTGGCAC TCACATGTTC TGCCTGAAGC CTAAGCTGAG GTCTGTACCC 
CCGGGCAACT GGTATTGCAA TGATTGCGTA AAGAGTCTGG GTCTCAGCAA TGGTCAAAAC 
GAAAAGGATA AAAAGCAGGC GACAAAGAAG AAACGCAAGT TTATCGTAGA AGAGGAGGAT 
GATGAAGCTA CAGACGAAGA AGAGGAAGAG AAAAAGGATG ACGATATGAC CGATGAGGAT 
GCGGAGCACG AGAACGAAAA ACACGATGAG GACGTTGAGG ACGACGAAAG TGTGACCTCT 
ACCCCCTCAT CTTCCAGAGT CAATGGGCGA ATTTTAAGGC GACCCCGCAC ACGGCCAACA 
AGCAGAAGAC TTACTTCGAA AGAGATCGAA GAACATGCTC AGGAAGACGT TGACAGCGGA 
GACGTCAGCG ATGACGCATC CTTAACCGCC GGTGAAGATA CGATCGAAGA CGAGTCAGAT 
GAGGAAAAGG TGTGTCAGAA GTGTTTCTAC GATGGCGGTG AAATCAAATG TGTGCAATGC 
AGGCTATTCT TTCACCTGGA ATGTGTTCAC CTCAAGCGAC CGCCTCGCAC AGATTTCGTT 
TGTAAAACCT GCAAGCCGAT GCCACAACGA CCTAGGCGCC GGCACAGTAA CATGAATGGT 
GATCATGACC GCGATGAGGA GGAGCCAAAA GCAAAGCGAC CACGTAACTC TTTGCGCCTA 
TCTATCGACA AGACCGCCCG CCCAAGCAAC GGCAACAACA ACAACAATAA TAACAACAGC 
AGCGTCAATA ACAACAACCA TCGCAGAAGC GGGCGTCGGA CGAACGAGCA CATGCCGTTG 
AACAGTGCCG CCTTGTACGA TCTGCTTGAG CAGATCATGA AGCACAAGGC CGCTTGGCCG 
TTTCTGCGAC CAGTGCTGAC ATCGGAGGTT CCCGACTACC ATCAGATCAT CAAAACGCCC 
ATGGATCTAG CTAAAATCAA ATCCAAGCTA AACATGGGCG CCTACCAGCT AAACGAGGAG 
CTGCTCAGCG ACATTCAGTT GGTGTTTAGA AACTGCGATT TGTACAACGT AGAAGGCAAC 
GAAATATACG ACGCTGGTTG TCAACTAGAA CGATTCGTGA TCGATCGATG TAGGGACATG 
CAGCTACCGT TTAGGCCTAG CGATATGAAC GGGGAAGTCA AAGCTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536734.2
CDS	388..4818
Translation	MPICKREGFDLNQKEGKNETFHDNDQVFCCYITKRIFRDYEHYFRHVMVINSTVWQCEATGKENLTYEEAVKSERAARKKMEQFKQSLRAPVLLVVEHAQQSAVNTLNMIVAKFLRKRYFIGEEVSVQAKKNATYTVLGVKLDKNMPEPLNGIYEDTDNLVYRLRPNKGDPSAELDLPFRQLRRSRMEFNLENLSMFIKSNVSRVDGLLRPKPEAYKQYVTDPGVNFSTIFIGKMPRYSPAKIKKPDGKKQSTLNKYIVAGEATAAKSKAKAKSDAKSLAEELERVKREKEAKLIELEKQKAEKKAQLIERVENECNLLLQKTDDLERTDQKVLPRYRQIVTLLPEHLLGDAFMMREFMHTYTGLLSGIEVFRQNLSFYEMTRALTAREIAGPLSDILLVLLGTVFDLQKEEEEECAVTYLDRAAQTQEPYWSMAQAAKSHLYAKRHFSFKVNELPLDALTLSEVLRLHLLGSGAFVNEKAERWRVMYRNGYSSKEDPGLELRLEHSHILRILKNHSVYQLKFKDIMLLIRCLMSQIMTYSGTINLIEERMEQTAKARQDLRALVVGENKRLAAVEINRKKLTQMHHLEVNGVEPEKREALVEKLKKSIAELHAQSDQQHRKHELQMLKLHSQLFNFLVYLGMDRCYRKYYVLESMPGIFVEHSPDSLDTCLEQPITNKSQIEIRQQSALPKNRKDLRVYLLKLYGDDEKKTKKKAKHSLENKENQEHRLNGSAEPMDVESDSPEAPTHFELLMCSGDKRSCIVHDSRNGQRQRWAYIYKAEEIDELIKALNPNGLREYELLQELSVLRSLIEQHAKTCPVDLLSLENETMRKKFMAAMESETNRKYGEANFGLPNGTDLNEVMRLHLVDRIIQFENDIYTGDLGRLKVKDMEKWRSDLLGGNYDAQCKLQWGPGGKLEDEAGSDNESHETHEEDDGALLGKYARKPYRDPGMYLAASADTKPLPDSDDEEDQHTNAVSIPIAVHNMASALLQVEQAIGKRFLKEPYGMKKWDPKQEALKLACDSRLHQWEVSLMESTSFAQVFLHLNILHDCIQWRRSTNKSLCKVCRRGSDPEKMLLCDECNAGTHMFCLKPKLRSVPPGNWYCNDCVKSLGLSNGQNEKDKKQATKKKRKFIVEEEDDEATDEEEEEKKDDDMTDEDAEHENEKHDEDVEDDESVTSTPSSSRVNGRILRRPRTRPTSRRLTSKEIEEHAQEDVDSGDVSDDASLTAGEDTIEDESDEEKVCQKCFYDGGEIKCVQCRLFFHLECVHLKRPPRTDFVCKTCKPMPQRPRRRHSNMNGDHDRDEEEPKAKRPRNSLRLSIDKTARPSNGNNNNNNNNSSVNNNNHRRSGRRTNEHMPLNSAALYDLLEQIMKHKAAWPFLRPVLTSEVPDYHQIIKTPMDLAKIKSKLNMGAYQLNEELLSDIQLVFRNCDLYNVEGNEIYDAGCQLERFVIDRCRDMQLPFRPSDMNGEVKAC

Target ORF information:

RefSeq Version	NM_080486.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ATP-dependent chromatin assembly factor large subunit (Acf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080486.3

ORF Insert Sequence:

ATGCCCATTT GCAAGCGGGA AGGATTCGAC CTGAATCAGA AGGAGGGCAA GAACGAAACA 
TTCCACGACA ACGACCAGGT CTTCTGCTGC TACATCACTA AGCGCATTTT TCGCGACTAT 
GAACACTACT TCCGGCACGT GATGGTGATC AACTCAACGG TGTGGCAGTG CGAGGCCACC 
GGCAAGGAGA ACCTCACCTA CGAGGAGGCG GTCAAAAGTG AGCGGGCGGC TCGCAAGAAG 
ATGGAGCAGT TCAAACAGAG CCTTCGGGCA CCCGTCCTTC TGGTTGTGGA GCACGCCCAA 
CAGTCGGCGG TCAACACCTT GAACATGATA GTGGCCAAGT TTCTGCGCAA GCGCTACTTT 
ATCGGCGAGG AGGTGTCGGT GCAAGCTAAG AAGAACGCCA CGTACACGGT GTTGGGTGTC 
AAGTTGGACA AAAATATGCC GGAACCACTC AATGGTATCT ATGAGGACAC GGACAACCTG 
GTCTACAGAC TACGACCAAA CAAGGGCGAT CCCAGTGCAG AGCTTGATCT GCCCTTTAGG 
CAATTACGTC GCTCGCGCAT GGAGTTTAAC CTGGAAAACC TGAGTATGTT TATAAAGAGC 
AATGTATCGC GGGTGGATGG ACTTCTTCGA CCCAAGCCAG AGGCATACAA GCAGTACGTG 
ACGGACCCTG GCGTAAACTT CTCCACAATT TTCATTGGCA AAATGCCACG TTATTCTCCC 
GCTAAGATTA AAAAACCTGA CGGCAAGAAG CAATCCACTC TCAACAAATA TATTGTCGCC 
GGTGAAGCAA CGGCTGCAAA GTCAAAGGCG AAGGCCAAGT CTGATGCTAA ATCTCTGGCA 
GAGGAATTGG AGCGGGTTAA GCGAGAAAAA GAGGCCAAGC TTATTGAGTT GGAAAAACAA 
AAGGCGGAAA AGAAGGCACA GTTGATTGAA CGCGTTGAAA ACGAATGTAA TTTACTGCTT 
CAAAAGACGG ACGACCTGGA GAGAACCGAC CAAAAAGTCC TGCCCCGCTA CAGACAGATC 
GTCACATTGC TGCCTGAGCA CCTCCTGGGT GATGCCTTCA TGATGCGCGA GTTTATGCAC 
ACTTACACAG GATTGCTGTC CGGCATCGAG GTGTTCCGTC AGAACCTCAG TTTCTACGAG 
ATGACACGTG CACTGACTGC TCGTGAGATT GCGGGCCCAC TATCGGACAT CCTCCTCGTT 
CTGCTTGGCA CTGTTTTCGA TCTGCAAAAA GAAGAGGAGG AGGAATGCGC AGTGACCTAC 
CTCGATCGGG CTGCACAAAC TCAGGAGCCC TATTGGAGTA TGGCGCAGGC CGCCAAAAGT 
CACCTTTACG CCAAGAGGCA CTTTTCCTTT AAGGTAAATG AACTACCATT AGATGCACTG 
ACTCTCAGTG AAGTGCTACG ACTGCATCTA CTTGGATCCG GAGCTTTTGT AAATGAAAAG 
GCGGAGCGCT GGAGAGTCAT GTACCGGAAT GGCTACTCGT CAAAAGAAGA CCCTGGTCTG 
GAACTACGTC TGGAACACTC GCACATCCTG CGCATTCTCA AAAATCATTC CGTATATCAG 
CTAAAGTTTA AGGATATAAT GCTCCTGATT CGCTGCCTGA TGTCCCAAAT TATGACATAC 
TCGGGCACGA TTAACTTGAT TGAGGAGCGT ATGGAGCAAA CAGCAAAGGC CAGGCAAGAT 
CTACGAGCTC TAGTTGTCGG GGAAAATAAG AGATTAGCCG CCGTAGAGAT AAACAGGAAA 
AAACTCACGC AAATGCACCA CCTGGAGGTA AATGGCGTTG AGCCTGAAAA GAGGGAAGCG 
TTGGTCGAGA AATTAAAGAA GAGTATTGCC GAGCTACACG CCCAATCGGA TCAGCAACAT 
CGCAAGCACG AGTTGCAGAT GCTTAAGTTG CATTCGCAGC TTTTCAATTT TTTGGTTTAC 
TTGGGCATGG ATAGGTGCTA CAGGAAATAT TACGTTTTGG AGTCTATGCC TGGGATCTTT 
GTCGAGCATT CGCCGGACAG CCTTGACACT TGTTTGGAGC AACCAATAAC AAATAAGTCC 
CAAATAGAAA TTCGTCAGCA GTCTGCATTG CCAAAGAATC GCAAGGACCT AAGGGTATAT 
CTACTCAAAC TCTACGGAGA CGACGAAAAG AAAACTAAGA AAAAGGCCAA GCACTCTCTA 
GAGAACAAGG AGAACCAAGA GCATCGCCTT AACGGCAGTG CAGAACCCAT GGATGTCGAA 
TCTGATTCTC CAGAGGCTCC CACTCATTTT GAGCTCTTGA TGTGCAGTGG TGACAAACGA 
TCTTGTATTG TGCATGATTC AAGAAATGGG CAACGGCAGC GTTGGGCATA TATTTACAAA 
GCCGAGGAGA TCGATGAATT AATTAAGGCT CTCAATCCCA ATGGTCTCCG TGAATATGAA 
CTCCTTCAAG AGCTATCCGT TCTGCGTTCC TTAATCGAAC AGCATGCTAA GACCTGCCCA 
GTAGACTTGC TAAGTCTAGA AAACGAAACA ATGCGCAAGA AGTTTATGGC GGCGATGGAA 
TCTGAAACTA ACCGTAAATA CGGAGAAGCA AACTTTGGGT TACCGAATGG GACCGATCTA 
AACGAAGTGA TGCGCTTGCA TTTAGTGGAT CGAATTATTC AATTTGAAAA TGATATTTAC 
ACTGGTGATC TCGGCCGCCT GAAAGTTAAG GATATGGAAA AGTGGCGAAG TGACTTGCTT 
GGAGGCAATT ACGACGCTCA GTGCAAGTTG CAGTGGGGCC CGGGTGGCAA GTTGGAAGAT 
GAAGCTGGTT CTGACAACGA ATCCCACGAA ACTCACGAGG AGGACGATGG CGCACTATTA 
GGTAAATATG CCAGGAAACC ATATCGCGAT CCTGGCATGT ATTTGGCAGC TTCTGCAGAC 
ACAAAACCCC TACCAGACAG CGACGATGAG GAGGATCAGC ATACGAATGC CGTCTCGATT 
CCTATCGCCG TGCATAACAT GGCCTCGGCC CTTCTGCAAG TGGAGCAGGC TATTGGAAAA 
CGGTTTCTGA AGGAACCCTA CGGCATGAAG AAGTGGGACC CCAAGCAGGA GGCTTTAAAA 
CTTGCCTGCG ACTCGCGTTT GCACCAGTGG GAAGTTTCGC TAATGGAAAG TACAAGCTTT 
GCTCAAGTCT TTCTGCACCT AAACATCTTG CACGACTGTA TTCAGTGGAG GCGTTCCACC 
AATAAGTCAT TATGCAAGGT CTGCAGGCGT GGCAGCGATC CGGAGAAGAT GCTGCTGTGC 
GATGAATGCA ACGCTGGCAC TCACATGTTC TGCCTGAAGC CTAAGCTGAG GTCTGTACCC 
CCGGGCAACT GGTATTGCAA TGATTGCGTA AAGAGTCTGG GTCTCAGCAA TGGTCAAAAC 
GAAAAGGATA AAAAGCAGGC GACAAAGAAG AAACGCAAGT TTATCGTAGA AGAGGAGGAT 
GATGAAGCTA CAGACGAAGA AGAGGAAGAG AAAAAGGATG ACGATATGAC CGATGAGGAT 
GCGGAGCACG AGAACGAAAA ACACGATGAG GACGTTGAGG ACGACGAAAG TGTGACCTCT 
ACCCCCTCAT CTTCCAGAGT CAATGGGCGA ATTTTAAGGC GACCCCGCAC ACGGCCAACA 
AGCAGAAGAC TTACTTCGAA AGAGATCGAA GAACATGCTC AGGAAGACGT TGACAGCGGA 
GACGTCAGCG ATGACGCATC CTTAACCGCC GGTGAAGATA CGATCGAAGA CGAGTCAGAT 
GAGGAAAAGG TGTGTCAGAA GTGTTTCTAC GATGGCGGTG AAATCAAATG TGTGCAATGC 
AGGCTATTCT TTCACCTGGA ATGTGTTCAC CTCAAGCGAC CGCCTCGCAC AGATTTCGTT 
TGTAAAACCT GCAAGCCGAT GCCACAACGA CCTAGGCGCC GGCACAGTAA CATGAATGGT 
GATCATGACC GCGATGAGGA GGAGCCAAAA GCAAAGCGAC CACGTAACTC TTTGCGCCTA 
TCTATCGACA AGACCGCCCG CCCAAGCAAC GGCAACAACA ACAACAATAA TAACAACAGC 
AGCGTCAATA ACAACAACCA TCGCAGAAGC GGGCGTCGGA CGAACGAGCA CATGCCGTTG 
AACAGTGCCG CCTTGTACGA TCTGCTTGAG CAGATCATGA AGCACAAGGC CGCTTGGCCG 
TTTCTGCGAC CAGTGCTGAC ATCGGAGGTT CCCGACTACC ATCAGATCAT CAAAACGCCC 
ATGGATCTAG CTAAAATCAA ATCCAAGCTA AACATGGGCG CCTACCAGCT AAACGAGGAG 
CTGCTCAGCG ACATTCAGTT GGTGTTTAGA AACTGCGATT TGTACAACGT AGAAGGCAAC 
GAAATATACG ACGCTGGTTG TCAACTAGAA CGATTCGTGA TCGATCGATG TAGGGACATG 
CAGCTACCGT TTAGGCCTAG CGATATGAAC GGGGAAGTCA AAGCTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Acf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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