pygo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pygo
Entrez Gene ID	43718
Full Name	pygopus
Synonyms	BEST:LD21971,CG11518,Dm Pygo,Dmel\CG11518,Pygo,anon-WO02077023.1,dPygo,gam,pNB5.2,pyg,pypo
General protein information	Preferred Names pygopus Names CG11518 gene product from transcript CG11518-RA CG11518-PA gammy legs pygo-PA pygopous
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100C6-100C6\|3-102 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_143615.4	NP_651872.1	pygopus

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors use a proteomics approach to discover the Chip/LDB-SSDP (ChiLS) complex as the ligand specifically binding to NPF motif of Pygo proteins.
Title: An ancient Pygo-dependent Wnt enhanceosome integrated by Chip/LDB-SSDP.

results are consistent with the idea that pygo functions independently of canonical Wnt signaling in the differentiation of the adult interchamber cardiac valves
Title: The role of pygopus in the differentiation of intracardiac valves in Drosophila.

humanized fly Pygo derepresses Notch targets, implying an inherent Notch-related function of classical Pygo orthologs, disabled in fly Pygo, which thus appears dedicated to Wnt signaling.
Title: Evolutionary adaptation of the fly Pygo PHD finger toward recognizing histone H3 tail methylated at arginine 2.

Double knockdown of pygo and Ca2+/calmodulin-dependent protein kinase II caused additional arrhythmia compared with pygo knockdown alone, suggesting that some of the effects of pygo are mediated by Ca2+ signaling.
Title: Pygopus maintains heart function in aging Drosophila independently of canonical Wnt signaling.

the PHD domain is required to link Drosophila Pygopus to Legless/beta-catenin and not to histone H3
Title: The PHD domain is required to link Drosophila Pygopus to Legless/beta-catenin and not to histone H3.

The following pygo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pygo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06766	NM_143615.4 Latest version!	Drosophila melanogaster pygopus (pygo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pygo ( NM_143615.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651872.1 Drosophila melanogaster pygopus (pygo), mRNA.

Price & Availability↑

CloneID	OFa06766
Clone ID Related Accession (Same CDS sequence)	NM_143615.4
Accession Version	NM_143615.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pygopus
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143615.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCACA ATCTTGGTAT GGCGCCATAT CGATTGCCGG GTCCAGCGGG CGGACTCTGT 
CCGCCCGATT TTAAGCCGCC GCCTCCCACG GACATCATCT CGGCGCCGAG CAATCCGAAG 
AAGCGGCGAA AAACCTCAAG TGCCGCCAAC TCCGCTGCAG CGGTGGCTGC GGCGGCGGCT 
GCAGCAGCTG CTGCGAATTC CATGCAGCAG CAGCAGGCGC CACCCACACC GCAGGATTTG 
CTGCCCCCTC CGCCAATGGG AGGCTTCGGA GACACCATTA TTGCCTCGAA TCCATTCGAC 
GACAGTCCCC AGGTGTCGGC GATGTCCAGC TCAGCGGCCG CGGCGATGGC GGCCATGAAT 
CAGATGGGCG GCGGACCAGG AGGTGGTCAC TTTGGCGGCG GTGGACCGGG TGGGCACCCG 
CACTGGGAAG ACCGCATGGG CATGGGCGGT GGACCTCCTC CCCCGCCTCA CATGCATCCC 
CATATGCACC CGCATCATCC AGGCGGACCT ATGGGTCACC CACATGGCCC ACATCCGCAC 
ATGGGTGGTC CACCTCCAAT GCGAGGAATG AGCCCCATGC ACCCCCATCA AATGGGACCG 
GGACCAGGCG TCGGACTACC GCCGCATATG AATCACGGAA GGCCAGGGGG ACCTGGTGGT 
CCTGGAGGAC CCGTCCCAAT GGGTAGTCCC ATGGGTGGAA TAGCTGGCAT GGGCGGCATG 
AGCCCAATGG GCGGAATGGG AGGCCCCAGC ATATCACCCC ATCACATGGG CATGGGTGGT 
CTGTCGCCCA TGGGAGGCGG TCCCAACGGA CCCAATCCGC GAGCCATGCA GGGTTCACCG 
ATGGGCGGTC CGGGGCAGAA CTCGCCAATG AACTCACTGC CTATGGGTTC GCCAATGGGC 
AATCCAATTG GCAGCCCGTT GGGCCCTCCC TCGGGACCGG GCCCTGGGAA TCCCGGCAAT 
ACCGGCGGAC CACAGCAGCA ACAACAACAA CCTCCGCAGC CACCGATGAA CAACGGGCAG 
ATGGGTCCTC CTCCTCTGCA CAGTCCGCTC GGAAACGGAC CAACGGGTCA TGGCAGTCAC 
ATGCCTGGAG GACCAATCCC AGGACCAGGT CCTGGGCCTG GCGGCCTAGT AGGTCCCGGT 
GGCATCTCCC CCGCGCACGG CAATAACCCG GGTGGTTCTG GGAACAACAT GCTCGGCGGG 
AATCCCGGCG GCGGCAACAG CAACAACAAC GGAAGCAATA CAAGTAACGC CAGCAACAAC 
AATCAAAATC CTCACCTCTC GCCAGCAGCC GGACGCCTGG GAGTGCCGAC GTCGATGCAG 
TCGAATGGAC CTTCGGTATC ATCGGTAGCC TCCTCATCGG TTCCCTCGCC CGCCACGCCC 
ACGCTCACGC CCACATCGAC GGCCACGTCC ATGTCCACGT CAGTGCCTAC ATCCTCGCCA 
GCGCCGCCCG CCATGTCACC GCATCACTCG CTAAACAGCG CCGGCCCGAG TCCGGGCATG 
CCCAACTCGG GACCCAGCCC GCTGCAGTCA CCAGCCGGAC CCAATGGCCC CAATAACAAC 
AACAGCAATA ACAACAACGG ACCCATGATG GGCCAGATGA TCCCGAACGC AGTTCCTATG 
CAGCACCAGC AGCACATGGG CGGCGGCCCA CCTGGCCACG GGCCCGGACC AATGCCCGGA 
ATGGGCATGA ACCAAATGCT GCCACCGCAG CAACCCTCCC ATCTTGGTCC CCCGCATCCG 
AATATGATGA ACCACCCGCA TCATCCGCAC CATCATCCTG GCGGACCACC GCCGCACATG 
ATGGGTGGAC CCGGAATGCA CGGCGGTCCT GCTGGAATGC CTCCTCATAT GGGCGGAGGA 
CCTAATCCGC ACATGATGGG CGGTCCGCAC GGGAACGCGG GTCCGCACAT GGGCCACGGC 
CACATGGGTG GAGTACCAGG TCCAGGACCC GGACCCGGCG GCATGAACGG ACCCCCGCAT 
CCGCACATGT CCCCGCACCA CGGACATCCG CATCACCACC ACAATCCGAT GGGCGGCCCA 
GGTCCAAATA TGTTCGGCGG TGGTGGAGGA GGTCCCATGG GTCCCGGTGG ACCGATGGGC 
AACATGGGGC CCATGGGAGG TGGCCCGATG GGCGGCCCTA TGGGCGTAGG TCCCAAGCCG 
ATGACAATGG GCGGCGGGAA GATGTACCCG CCGGGACAGC CAATGGTCTT TAATCCGCAG 
AACCCGAATG CGCCGCCCAT ATATCCTTGT GGCATGTGCC ACAAGGAGGT GAACGACAAC 
GACGAAGCCG TGTTCTGTGA ATCCGGTTGT AACTTTTTCT TTCACAGAAC CTGTGTTGGC 
CTGACAGAGG CGGCCTTCCA AATGCTCAAC AAGGAGGTGT TTGCCGAGTG GTGCTGCGAC 
AAGTGCGTGT CTTCCAAGCA TATTCCCATG GTCAAGTTCA AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651872.1
CDS	1003..3450
Translation	MTHNLGMAPYRLPGPAGGLCPPDFKPPPPTDIISAPSNPKKRRKTSSAANSAAAVAAAAAAAAAANSMQQQQAPPTPQDLLPPPPMGGFGDTIIASNPFDDSPQVSAMSSSAAAAMAAMNQMGGGPGGGHFGGGGPGGHPHWEDRMGMGGGPPPPPHMHPHMHPHHPGGPMGHPHGPHPHMGGPPPMRGMSPMHPHQMGPGPGVGLPPHMNHGRPGGPGGPGGPVPMGSPMGGIAGMGGMSPMGGMGGPSISPHHMGMGGLSPMGGGPNGPNPRAMQGSPMGGPGQNSPMNSLPMGSPMGNPIGSPLGPPSGPGPGNPGNTGGPQQQQQQPPQPPMNNGQMGPPPLHSPLGNGPTGHGSHMPGGPIPGPGPGPGGLVGPGGISPAHGNNPGGSGNNMLGGNPGGGNSNNNGSNTSNASNNNQNPHLSPAAGRLGVPTSMQSNGPSVSSVASSSVPSPATPTLTPTSTATSMSTSVPTSSPAPPAMSPHHSLNSAGPSPGMPNSGPSPLQSPAGPNGPNNNNSNNNNGPMMGQMIPNAVPMQHQQHMGGGPPGHGPGPMPGMGMNQMLPPQQPSHLGPPHPNMMNHPHHPHHHPGGPPPHMMGGPGMHGGPAGMPPHMGGGPNPHMMGGPHGNAGPHMGHGHMGGVPGPGPGPGGMNGPPHPHMSPHHGHPHHHHNPMGGPGPNMFGGGGGGPMGPGGPMGNMGPMGGGPMGGPMGVGPKPMTMGGGKMYPPGQPMVFNPQNPNAPPIYPCGMCHKEVNDNDEAVFCESGCNFFFHRTCVGLTEAAFQMLNKEVFAEWCCDKCVSSKHIPMVKFKC

Target ORF information:

RefSeq Version	NM_143615.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pygopus (pygo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143615.4

ORF Insert Sequence:

ATGACCCACA ATCTTGGTAT GGCGCCATAT CGATTGCCGG GTCCAGCGGG CGGACTCTGT 
CCGCCCGATT TTAAGCCGCC GCCTCCCACG GACATCATCT CGGCGCCGAG CAATCCGAAG 
AAGCGGCGAA AAACCTCAAG TGCCGCCAAC TCCGCTGCAG CGGTGGCTGC GGCGGCGGCT 
GCAGCAGCTG CTGCGAATTC CATGCAGCAG CAGCAGGCGC CACCCACACC GCAGGATTTG 
CTGCCCCCTC CGCCAATGGG AGGCTTCGGA GACACCATTA TTGCCTCGAA TCCATTCGAC 
GACAGTCCCC AGGTGTCGGC GATGTCCAGC TCAGCGGCCG CGGCGATGGC GGCCATGAAT 
CAGATGGGCG GCGGACCAGG AGGTGGTCAC TTTGGCGGCG GTGGACCGGG TGGGCACCCG 
CACTGGGAAG ACCGCATGGG CATGGGCGGT GGACCTCCTC CCCCGCCTCA CATGCATCCC 
CATATGCACC CGCATCATCC AGGCGGACCT ATGGGTCACC CACATGGCCC ACATCCGCAC 
ATGGGTGGTC CACCTCCAAT GCGAGGAATG AGCCCCATGC ACCCCCATCA AATGGGACCG 
GGACCAGGCG TCGGACTACC GCCGCATATG AATCACGGAA GGCCAGGGGG ACCTGGTGGT 
CCTGGAGGAC CCGTCCCAAT GGGTAGTCCC ATGGGTGGAA TAGCTGGCAT GGGCGGCATG 
AGCCCAATGG GCGGAATGGG AGGCCCCAGC ATATCACCCC ATCACATGGG CATGGGTGGT 
CTGTCGCCCA TGGGAGGCGG TCCCAACGGA CCCAATCCGC GAGCCATGCA GGGTTCACCG 
ATGGGCGGTC CGGGGCAGAA CTCGCCAATG AACTCACTGC CTATGGGTTC GCCAATGGGC 
AATCCAATTG GCAGCCCGTT GGGCCCTCCC TCGGGACCGG GCCCTGGGAA TCCCGGCAAT 
ACCGGCGGAC CACAGCAGCA ACAACAACAA CCTCCGCAGC CACCGATGAA CAACGGGCAG 
ATGGGTCCTC CTCCTCTGCA CAGTCCGCTC GGAAACGGAC CAACGGGTCA TGGCAGTCAC 
ATGCCTGGAG GACCAATCCC AGGACCAGGT CCTGGGCCTG GCGGCCTAGT AGGTCCCGGT 
GGCATCTCCC CCGCGCACGG CAATAACCCG GGTGGTTCTG GGAACAACAT GCTCGGCGGG 
AATCCCGGCG GCGGCAACAG CAACAACAAC GGAAGCAATA CAAGTAACGC CAGCAACAAC 
AATCAAAATC CTCACCTCTC GCCAGCAGCC GGACGCCTGG GAGTGCCGAC GTCGATGCAG 
TCGAATGGAC CTTCGGTATC ATCGGTAGCC TCCTCATCGG TTCCCTCGCC CGCCACGCCC 
ACGCTCACGC CCACATCGAC GGCCACGTCC ATGTCCACGT CAGTGCCTAC ATCCTCGCCA 
GCGCCGCCCG CCATGTCACC GCATCACTCG CTAAACAGCG CCGGCCCGAG TCCGGGCATG 
CCCAACTCGG GACCCAGCCC GCTGCAGTCA CCAGCCGGAC CCAATGGCCC CAATAACAAC 
AACAGCAATA ACAACAACGG ACCCATGATG GGCCAGATGA TCCCGAACGC AGTTCCTATG 
CAGCACCAGC AGCACATGGG CGGCGGCCCA CCTGGCCACG GGCCCGGACC AATGCCCGGA 
ATGGGCATGA ACCAAATGCT GCCACCGCAG CAACCCTCCC ATCTTGGTCC CCCGCATCCG 
AATATGATGA ACCACCCGCA TCATCCGCAC CATCATCCTG GCGGACCACC GCCGCACATG 
ATGGGTGGAC CCGGAATGCA CGGCGGTCCT GCTGGAATGC CTCCTCATAT GGGCGGAGGA 
CCTAATCCGC ACATGATGGG CGGTCCGCAC GGGAACGCGG GTCCGCACAT GGGCCACGGC 
CACATGGGTG GAGTACCAGG TCCAGGACCC GGACCCGGCG GCATGAACGG ACCCCCGCAT 
CCGCACATGT CCCCGCACCA CGGACATCCG CATCACCACC ACAATCCGAT GGGCGGCCCA 
GGTCCAAATA TGTTCGGCGG TGGTGGAGGA GGTCCCATGG GTCCCGGTGG ACCGATGGGC 
AACATGGGGC CCATGGGAGG TGGCCCGATG GGCGGCCCTA TGGGCGTAGG TCCCAAGCCG 
ATGACAATGG GCGGCGGGAA GATGTACCCG CCGGGACAGC CAATGGTCTT TAATCCGCAG 
AACCCGAATG CGCCGCCCAT ATATCCTTGT GGCATGTGCC ACAAGGAGGT GAACGACAAC 
GACGAAGCCG TGTTCTGTGA ATCCGGTTGT AACTTTTTCT TTCACAGAAC CTGTGTTGGC 
CTGACAGAGG CGGCCTTCCA AATGCTCAAC AAGGAGGTGT TTGCCGAGTG GTGCTGCGAC 
AAGTGCGTGT CTTCCAAGCA TATTCCCATG GTCAAGTTCA AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pygo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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