eIF4H2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF4H2
Entrez Gene ID	43645
Full Name	CG1340 gene product from transcript CG1340-RC
Synonyms	Dmel\CG1340,cg1340,eIF4H
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100A3-100A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276193.1	NP_001263122.1	eukaryotic translation initiation factor 4H2, isoform C
NM_143563.3	NP_651820.1	eukaryotic translation initiation factor 4H2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eIF4H2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF4H2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06689	NM_143563.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4H2 (eIF4H2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06689	NM_001276193.1 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4H2 (eIF4H2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF4H2 ( NM_001276193.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263122.1 Drosophila melanogaster eukaryotic translation initiation factor 4H2 (eIF4H2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06689
Clone ID Related Accession (Same CDS sequence)	NM_001276193.1 , NM_143563.3
Accession Version	NM_001276193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4H2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAATC GCTCCAGCCA GCGGGACCGA GAGTTCAATC GTGATAATAC TTACAATAAC 
AATCGCGACA ATCGAGACCA TAATCGGGAC AATCGCGATA ATCGGGAGAA TCGCAGTAAT 
TTCCAAAACT ATCGCAATCG TGACAGGGAT CGCGATAGGC AGCGTCAGGT GCCCACGGAA 
CCTCCGTTCA TCGCTTATGT GGGCAACCTT CCCAAGGGTC TCGTCCAGGG CGATGTGATG 
AAGATATTTT CGGACTTCGA GGTGAAGAAT GTGCGCCTGA TCAAGGACCG TGAGACGGAT 
GAATTCAAAG GCTATGGCTA CGTAGAGTTC GAGACTCTGG CACAACTGAA GAGTGCTCTC 
AACTGCAACG GACGCATCAA GCTAGACAAC TTTTCGGCCC CCCTGCGTAT CGATATAGCC 
GACCATCGCC GGCAGAACCC GGGTGCTCCC AGCGGTGTTG GCGGAGCTCC TCCAGCCGGA 
GTGGGCCACG AAAGAGGTGG TGGCGTCGGA AGAGGAGGCA GCACGGGTGC AGCAAACGGT 
AACAATCCCT ACTACCAGAG GCGCAACTAT CGGCGGGACG ACAGTGTGGG ATCCCATCAG 
TTTAGAAGGA GGGAACCGCG CAGCAACAGC AGCAACCACC AAATGTCCAA TAGCAGCCCC 
ACACAAAGCA CCACGTCCAT TAATTACAAC CGCACCACTC GAGGTGGTTT CAATAGCCGT 
GTTGCTGTAG GTGGAAATGG CAATCGTTAC CAAGGAGGAG CTCCACGTAA CTTCAATGAC 
CGCGAGGATC AGCAATCGAC CGGCAGCGGA GGATTCCAAC GCAATCGCAC CTACAACTTT 
AACCGATCCA TGAACAACAG CGTCGGCGGA GGAGGTGCTG GAGTAGGTAG GGGCGGGCAT 
CCGCAGCGGA ACTTCAATGG AAATGGAGGC GGCGGTGGAT TCGGGGTCGT AAATGGAGGC 
GGCAACTATA CCAATTTTGT GCAGAATCGC AATCGCGATC GCCGTGGACA CTACAATCCA 
AATCACTCTG GAAGTTCTGG CGGCTACAAT AACAATGGGA ACAACACCTT TGGCGGGGAG 
AACAATCATC CTGTGCACGG AGCATCCGAG TCGGCATCAT CTGGAACAGG TCCTAGTTCG 
AGCAATCAAT TCGCCGTACG CGACGATGAT GATCGGCCCA AGCTAGTGCT CAAGCCCAGG 
ACTGTGACTG CACCCATCAA CTCTCTGGCT GAAACCAAAC AGGCTGCTCT CATCTTCGGA 
AAGGCCAAGC CCCGTGATGA TAGCTCCACG CCCGTCTCCT CGCCACGCCA GGATTTGGAC 
GCAGGATCGT CAGGTGCTCC AAGTGATCAT GATCATGATC CAGGCTTGGG CGACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263122.1
CDS	224..1603
Translation	MANRSSQRDREFNRDNTYNNNRDNRDHNRDNRDNRENRSNFQNYRNRDRDRDRQRQVPTEPPFIAYVGNLPKGLVQGDVMKIFSDFEVKNVRLIKDRETDEFKGYGYVEFETLAQLKSALNCNGRIKLDNFSAPLRIDIADHRRQNPGAPSGVGGAPPAGVGHERGGGVGRGGSTGAANGNNPYYQRRNYRRDDSVGSHQFRRREPRSNSSNHQMSNSSPTQSTTSINYNRTTRGGFNSRVAVGGNGNRYQGGAPRNFNDREDQQSTGSGGFQRNRTYNFNRSMNNSVGGGGAGVGRGGHPQRNFNGNGGGGGFGVVNGGGNYTNFVQNRNRDRRGHYNPNHSGSSGGYNNNGNNTFGGENNHPVHGASESASSGTGPSSSNQFAVRDDDDRPKLVLKPRTVTAPINSLAETKQAALIFGKAKPRDDSSTPVSSPRQDLDAGSSGAPSDHDHDPGLGDN

Target ORF information:

RefSeq Version	NM_001276193.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4H2 (eIF4H2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276193.1

ORF Insert Sequence:

ATGGCCAATC GCTCCAGCCA GCGGGACCGA GAGTTCAATC GTGATAATAC TTACAATAAC 
AATCGCGACA ATCGAGACCA TAATCGGGAC AATCGCGATA ATCGGGAGAA TCGCAGTAAT 
TTCCAAAACT ATCGCAATCG TGACAGGGAT CGCGATAGGC AGCGTCAGGT GCCCACGGAA 
CCTCCGTTCA TCGCTTATGT GGGCAACCTT CCCAAGGGTC TCGTCCAGGG CGATGTGATG 
AAGATATTTT CGGACTTCGA GGTGAAGAAT GTGCGCCTGA TCAAGGACCG TGAGACGGAT 
GAATTCAAAG GCTATGGCTA CGTAGAGTTC GAGACTCTGG CACAACTGAA GAGTGCTCTC 
AACTGCAACG GACGCATCAA GCTAGACAAC TTTTCGGCCC CCCTGCGTAT CGATATAGCC 
GACCATCGCC GGCAGAACCC GGGTGCTCCC AGCGGTGTTG GCGGAGCTCC TCCAGCCGGA 
GTGGGCCACG AAAGAGGTGG TGGCGTCGGA AGAGGAGGCA GCACGGGTGC AGCAAACGGT 
AACAATCCCT ACTACCAGAG GCGCAACTAT CGGCGGGACG ACAGTGTGGG ATCCCATCAG 
TTTAGAAGGA GGGAACCGCG CAGCAACAGC AGCAACCACC AAATGTCCAA TAGCAGCCCC 
ACACAAAGCA CCACGTCCAT TAATTACAAC CGCACCACTC GAGGTGGTTT CAATAGCCGT 
GTTGCTGTAG GTGGAAATGG CAATCGTTAC CAAGGAGGAG CTCCACGTAA CTTCAATGAC 
CGCGAGGATC AGCAATCGAC CGGCAGCGGA GGATTCCAAC GCAATCGCAC CTACAACTTT 
AACCGATCCA TGAACAACAG CGTCGGCGGA GGAGGTGCTG GAGTAGGTAG GGGCGGGCAT 
CCGCAGCGGA ACTTCAATGG AAATGGAGGC GGCGGTGGAT TCGGGGTCGT AAATGGAGGC 
GGCAACTATA CCAATTTTGT GCAGAATCGC AATCGCGATC GCCGTGGACA CTACAATCCA 
AATCACTCTG GAAGTTCTGG CGGCTACAAT AACAATGGGA ACAACACCTT TGGCGGGGAG 
AACAATCATC CTGTGCACGG AGCATCCGAG TCGGCATCAT CTGGAACAGG TCCTAGTTCG 
AGCAATCAAT TCGCCGTACG CGACGATGAT GATCGGCCCA AGCTAGTGCT CAAGCCCAGG 
ACTGTGACTG CACCCATCAA CTCTCTGGCT GAAACCAAAC AGGCTGCTCT CATCTTCGGA 
AAGGCCAAGC CCCGTGATGA TAGCTCCACG CCCGTCTCCT CGCCACGCCA GGATTTGGAC 
GCAGGATCGT CAGGTGCTCC AAGTGATCAT GATCATGATC CAGGCTTGGG CGACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4H2 ( NM_143563.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651820.1 Drosophila melanogaster eukaryotic translation initiation factor 4H2 (eIF4H2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06689
Clone ID Related Accession (Same CDS sequence)	NM_001276193.1 , NM_143563.3
Accession Version	NM_143563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4H2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143563.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAATC GCTCCAGCCA GCGGGACCGA GAGTTCAATC GTGATAATAC TTACAATAAC 
AATCGCGACA ATCGAGACCA TAATCGGGAC AATCGCGATA ATCGGGAGAA TCGCAGTAAT 
TTCCAAAACT ATCGCAATCG TGACAGGGAT CGCGATAGGC AGCGTCAGGT GCCCACGGAA 
CCTCCGTTCA TCGCTTATGT GGGCAACCTT CCCAAGGGTC TCGTCCAGGG CGATGTGATG 
AAGATATTTT CGGACTTCGA GGTGAAGAAT GTGCGCCTGA TCAAGGACCG TGAGACGGAT 
GAATTCAAAG GCTATGGCTA CGTAGAGTTC GAGACTCTGG CACAACTGAA GAGTGCTCTC 
AACTGCAACG GACGCATCAA GCTAGACAAC TTTTCGGCCC CCCTGCGTAT CGATATAGCC 
GACCATCGCC GGCAGAACCC GGGTGCTCCC AGCGGTGTTG GCGGAGCTCC TCCAGCCGGA 
GTGGGCCACG AAAGAGGTGG TGGCGTCGGA AGAGGAGGCA GCACGGGTGC AGCAAACGGT 
AACAATCCCT ACTACCAGAG GCGCAACTAT CGGCGGGACG ACAGTGTGGG ATCCCATCAG 
TTTAGAAGGA GGGAACCGCG CAGCAACAGC AGCAACCACC AAATGTCCAA TAGCAGCCCC 
ACACAAAGCA CCACGTCCAT TAATTACAAC CGCACCACTC GAGGTGGTTT CAATAGCCGT 
GTTGCTGTAG GTGGAAATGG CAATCGTTAC CAAGGAGGAG CTCCACGTAA CTTCAATGAC 
CGCGAGGATC AGCAATCGAC CGGCAGCGGA GGATTCCAAC GCAATCGCAC CTACAACTTT 
AACCGATCCA TGAACAACAG CGTCGGCGGA GGAGGTGCTG GAGTAGGTAG GGGCGGGCAT 
CCGCAGCGGA ACTTCAATGG AAATGGAGGC GGCGGTGGAT TCGGGGTCGT AAATGGAGGC 
GGCAACTATA CCAATTTTGT GCAGAATCGC AATCGCGATC GCCGTGGACA CTACAATCCA 
AATCACTCTG GAAGTTCTGG CGGCTACAAT AACAATGGGA ACAACACCTT TGGCGGGGAG 
AACAATCATC CTGTGCACGG AGCATCCGAG TCGGCATCAT CTGGAACAGG TCCTAGTTCG 
AGCAATCAAT TCGCCGTACG CGACGATGAT GATCGGCCCA AGCTAGTGCT CAAGCCCAGG 
ACTGTGACTG CACCCATCAA CTCTCTGGCT GAAACCAAAC AGGCTGCTCT CATCTTCGGA 
AAGGCCAAGC CCCGTGATGA TAGCTCCACG CCCGTCTCCT CGCCACGCCA GGATTTGGAC 
GCAGGATCGT CAGGTGCTCC AAGTGATCAT GATCATGATC CAGGCTTGGG CGACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651820.1
CDS	224..1603
Translation	MANRSSQRDREFNRDNTYNNNRDNRDHNRDNRDNRENRSNFQNYRNRDRDRDRQRQVPTEPPFIAYVGNLPKGLVQGDVMKIFSDFEVKNVRLIKDRETDEFKGYGYVEFETLAQLKSALNCNGRIKLDNFSAPLRIDIADHRRQNPGAPSGVGGAPPAGVGHERGGGVGRGGSTGAANGNNPYYQRRNYRRDDSVGSHQFRRREPRSNSSNHQMSNSSPTQSTTSINYNRTTRGGFNSRVAVGGNGNRYQGGAPRNFNDREDQQSTGSGGFQRNRTYNFNRSMNNSVGGGGAGVGRGGHPQRNFNGNGGGGGFGVVNGGGNYTNFVQNRNRDRRGHYNPNHSGSSGGYNNNGNNTFGGENNHPVHGASESASSGTGPSSSNQFAVRDDDDRPKLVLKPRTVTAPINSLAETKQAALIFGKAKPRDDSSTPVSSPRQDLDAGSSGAPSDHDHDPGLGDN

Target ORF information:

RefSeq Version	NM_143563.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4H2 (eIF4H2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143563.3

ORF Insert Sequence:

ATGGCCAATC GCTCCAGCCA GCGGGACCGA GAGTTCAATC GTGATAATAC TTACAATAAC 
AATCGCGACA ATCGAGACCA TAATCGGGAC AATCGCGATA ATCGGGAGAA TCGCAGTAAT 
TTCCAAAACT ATCGCAATCG TGACAGGGAT CGCGATAGGC AGCGTCAGGT GCCCACGGAA 
CCTCCGTTCA TCGCTTATGT GGGCAACCTT CCCAAGGGTC TCGTCCAGGG CGATGTGATG 
AAGATATTTT CGGACTTCGA GGTGAAGAAT GTGCGCCTGA TCAAGGACCG TGAGACGGAT 
GAATTCAAAG GCTATGGCTA CGTAGAGTTC GAGACTCTGG CACAACTGAA GAGTGCTCTC 
AACTGCAACG GACGCATCAA GCTAGACAAC TTTTCGGCCC CCCTGCGTAT CGATATAGCC 
GACCATCGCC GGCAGAACCC GGGTGCTCCC AGCGGTGTTG GCGGAGCTCC TCCAGCCGGA 
GTGGGCCACG AAAGAGGTGG TGGCGTCGGA AGAGGAGGCA GCACGGGTGC AGCAAACGGT 
AACAATCCCT ACTACCAGAG GCGCAACTAT CGGCGGGACG ACAGTGTGGG ATCCCATCAG 
TTTAGAAGGA GGGAACCGCG CAGCAACAGC AGCAACCACC AAATGTCCAA TAGCAGCCCC 
ACACAAAGCA CCACGTCCAT TAATTACAAC CGCACCACTC GAGGTGGTTT CAATAGCCGT 
GTTGCTGTAG GTGGAAATGG CAATCGTTAC CAAGGAGGAG CTCCACGTAA CTTCAATGAC 
CGCGAGGATC AGCAATCGAC CGGCAGCGGA GGATTCCAAC GCAATCGCAC CTACAACTTT 
AACCGATCCA TGAACAACAG CGTCGGCGGA GGAGGTGCTG GAGTAGGTAG GGGCGGGCAT 
CCGCAGCGGA ACTTCAATGG AAATGGAGGC GGCGGTGGAT TCGGGGTCGT AAATGGAGGC 
GGCAACTATA CCAATTTTGT GCAGAATCGC AATCGCGATC GCCGTGGACA CTACAATCCA 
AATCACTCTG GAAGTTCTGG CGGCTACAAT AACAATGGGA ACAACACCTT TGGCGGGGAG 
AACAATCATC CTGTGCACGG AGCATCCGAG TCGGCATCAT CTGGAACAGG TCCTAGTTCG 
AGCAATCAAT TCGCCGTACG CGACGATGAT GATCGGCCCA AGCTAGTGCT CAAGCCCAGG 
ACTGTGACTG CACCCATCAA CTCTCTGGCT GAAACCAAAC AGGCTGCTCT CATCTTCGGA 
AAGGCCAAGC CCCGTGATGA TAGCTCCACG CCCGTCTCCT CGCCACGCCA GGATTTGGAC 
GCAGGATCGT CAGGTGCTCC AAGTGATCAT GATCATGATC CAGGCTTGGG CGACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF4H2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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