Usp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp1
Entrez Gene ID	43462
Full Name	Ubiquitin specific protease 1
Synonyms	BcDNA:LD22910,CG15817,Dmel\CG15817,LD22910,Q8I077,usp1
General protein information	Preferred Names Ubiquitin specific protease 1 Names CG15817 gene product from transcript CG15817-RE CG15817-PC CG15817-PD CG15817-PE Usp1-PC Usp1-PD Usp1-PE ubiquitin specific protease 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99A1-99A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143430.4	NP_651687.3	ubiquitin specific protease 1, isoform D
NM_001276132.1	NP_001263061.1	ubiquitin specific protease 1, isoform E
NM_170403.3	NP_733282.1	ubiquitin specific protease 1, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Usp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06485	NM_143430.4 Latest version!	Drosophila melanogaster ubiquitin specific protease 1, transcript variant D (Usp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa06485	NM_170403.3 Latest version!	Drosophila melanogaster ubiquitin specific protease 1, transcript variant C (Usp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa06485	NM_001276132.1 Latest version!	Drosophila melanogaster ubiquitin specific protease 1, transcript variant E (Usp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp1 ( NM_143430.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651687.3 Drosophila melanogaster ubiquitin specific protease 1, transcript variant D (Usp1), mRNA.

Price & Availability↑

CloneID	OFa06485
Clone ID Related Accession (Same CDS sequence)	NM_143430.4 , NM_001276132.1 , NM_170403.3
Accession Version	NM_143430.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143430.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAT ATGACGTAAA TGAGCGCAAA GAGGAACCAG CCAGTCTTCC ACCCACACAA 
AACGAGACCA CTGAAATCAA CAGCCAGCTA CGACAGGATC AGCAAAATCA GGAACAGGAG 
AATCCCCTAC GGCAGCAGGA TAACAGCCAC CCGCCTCAGG AAAATCCAAT TAATCAGCAG 
GAGAAGGACA CATTGCAAGT TTCCGTTCTT TATCCTCTGG TAGTCTGCGC GCAGCTGGCG 
GACAGTCAAC TGGTTCTGAC TGCGGACACG CCGAAGCTAT CTCCAGTTAA AAAGCGTCAG 
CGGCGCGTTT ATGTTGCGGA CACCTTGCGC TGCTACAGTC TACGTTCCCG GCAAGAGCCC 
GCGACCAAGG ACCCACTCGA AGGTGGCCTA AAGCAGCCGG ACGTCGTTCC ACCACCCCCG 
CCTGTCCAGG CGCCCTCTCC CACGCCCACA GAGTCCACAG AATACACACC TCCAGAACAA 
TTTAAATCGG AGTTTTGGAA CAGAATTGAA GAGGCTCAAG AGCCAAAGCC TGTTGGAAAG 
AAGCGACGAA CCCCTGGAGG ACTCAAAAGA ACGCGGGGCA ACAAGCTTCT AAAAGAGCTC 
GAGATCCAAT CGCCGGAAAC GGCGGCCGCT ATTGCGGCAG CACAGGCGGA ACTCAACGCC 
GCAGGAAAGT CGGGAGCAGC AGGAGCACGG TCCAGCCGCA AGAGGACCAA CACGATGTCT 
CTATACGATC GGCGATTCCA GACGACCACC GAGGAGCGCC GCGTGGCTAA CGGATACGGT 
GGCAATGCTG CGCGATCGGT CACTGGCTCC TCGTCAAACG AGATCGAGTC CCAGGAGCCG 
CAGAGTCATC AGTATTACGG TCCCAATGGC GGCAGCAATG CCGCGGGAGC AGGCGCAGGC 
TTGAACGGAA GTGCGGCGGC CCTGAACCAT GCCCCTTCCA TGGGCACACT GTGCAACATT 
GGCAACAGTT GCTACTTGAA TTCTGTGGTT TACACCCTGC GCTTTGCCCC GCACTTTTTG 
CACAACCTAC ACCACCTGAT ACAGGACTTG AATGTTGTGC AGCAGACGAT AGTTCGCCAA 
CAAACGGCCA GGTCTGCGTC CCTCGGCAAA AATGTGAGTG CCGCTCAGTT GGAGCACGCC 
CGCAGCTGGA GCAGCAAGGA TCTGGCCACC AGTACGGATC AGTACAGTGG CCAGAATGGC 
GGAGTTAATA GCGGAAACGG TGGTAGCGGC TCGAGCAAGT CGACTCATCA GTCGGTAACC 
GAAAAACTCC ATGAACTGTA TAACAACCTG CACGGCAACG AGATGGCAGA CTCCACGGAG 
CCCTACCACG CGGACACGCT GCTGCACGCC ATTCAGGACG TAAACGCCAC CTTTGAAGGT 
AACCAGCAGC AGGACGCACA CGAATTCCTA ATGTGCGTGC TTAATTGCAT CAGGGAAACG 
AATCAGTCTT TGATCAAGGC CATTGGCGAG TGTCCTGAGG TCATCGCAAA TGGATACATA 
GCCAACCCGG ATGAGGTCGA CACCGGAGAG GGACAGGATC GCACTGATTC GACTGCGTCG 
CAGAACCTGA ACGCTGGCAA TGGATCGCTG GCAACTAGTC AAACAACCAC GACCAAAACG 
TCGTTCTTCT CTCGCAAATC AAAAAGAAAA GACGAGGTCA AGCCATCCAA GTCGACGCGC 
GTTCAGTCGC CACTCAAGGA GAATAGTCCC ACGGCGGGCG GGATAACCGG TGCGGGCACA 
GCCCATGCCA CCGCCAACAG CCTGTTCTAC CTGAACACAG TTGATCTCAG CGGCGCCAGC 
AGCACCAGCG GTAGTGCCAG TACGAGTGCC AGTGGCGTTG TTTCCACAAG TGCAGCGTTG 
CCCACACCCC CGCAGGCTAC AAAATACTCC AGTGACGACG AGATGAATTC CGCCACCGTG 
CTGAAGGACA AAATGCGACT GGAGGAGCGA ATACGGGAGC TCAATCTAAA CTTCTTCAGC 
TCGGACTTCG AAGGCATTGT GGTGCTGACC ACCAAGTGCC TCAGTTGCGA GACGATAACG 
CGGCAAAAGC AGGGCATGCT TGATATTTCT GTGCCGGTGC CGATCTCGGG GTACGACAAT 
GCTGATCTCC AAGACAAACC GAGCACCTAC ATACAGAATT CCTGCATTAC GAAAGAGTAT 
TTCCGCGGGG AGAATAAGTA TAGTTGCAAC CAGTGCACCG GCTACACCGA AGCCATCCGC 
TCCATCTCGT ACGAGGTGCT GCCGCGGTTA CTGGTCATTC AGCTGAATCG CTTCTCTGGC 
GGCATGGAGA AAGTGAGTAC GTACGTGCCG ACCACCTTCA CTCTGCCCTG TTTCTGCGCC 
ACCTGCTGTG AGCTGGGCGA GGGCAACAAG CTGCACGTTT ACAAGCTGTA CAGCGTGATC 
ACGCATGTGG GAGCCACCCT GACGGTGGGC CACTACATCG CCTATACGTG CTTCTTGGAT 
CTCGCCAGTG ACTATGTCAA CTGCCCTAAG GATCGCAGGA ATACGATGAC GAACTCGCAA 
ACGATGGCTT CGCAAGCAGT GCCCTCCAAT GAGAATGCTG CGCCAAACAA TGGTAGCAGC 
TCCGTGGCCA GCACTCCAGT TATTGCAGCA GCTTCGGCAT TGGCCTCATC GGGCAACATT 
TTGATGAAGA AGATGAAGTT CGGTCGCAGC AAGGCCTCCA GCAGTGGTGA TATGAGCAAG 
AACGTTAAGC AGGTTAATGG GATAATCAGC AAGAACATCA CCAATGGAAT TGGCAAGCTG 
AGCATAAGCA CCACCTGCCA AGGAGTCAAC TGCTGTGCCA TGAGGCTGTG TTGCACCCAG 
CAGACGAGCA GCAGCAACTC GACCAGTTGC AGTGATTTCA GCGAGGAGTC CCTGCAAAAC 
GGAAGCAACA GCAATCTCAG CTTCGGAGGA TCGGGCAGCA CCTATCCCAC GGGCTACGGA 
AGCACGGGTC GCGGTGGTGT CAGAGCCAAC TACGCCCACG GAGGTCCTGA TCCCATTTGG 
TATATGTGCG ACGACGACAA GATCAAGGCG ATGACCCAAA GGGAGTTCGA AGAGCTGCTA 
TCGCCCACAC GGAAGATCAC AATAACGCCG TATTTGCTCT TTTACGCGCG ATTCGATCTG 
CAGCCGCAGA AGGCGACGCC GCCGATGCCT GGATCATCCA CAACGCCTCC GCCACCGTCG 
TCGGCACAGA GTTCGTGGTC GAATGAAAAC GTGTCCAGCG GCTCGCACAA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651687.3
CDS	429..3665
Translation	MHEYDVNERKEEPASLPPTQNETTEINSQLRQDQQNQEQENPLRQQDNSHPPQENPINQQEKDTLQVSVLYPLVVCAQLADSQLVLTADTPKLSPVKKRQRRVYVADTLRCYSLRSRQEPATKDPLEGGLKQPDVVPPPPPVQAPSPTPTESTEYTPPEQFKSEFWNRIEEAQEPKPVGKKRRTPGGLKRTRGNKLLKELEIQSPETAAAIAAAQAELNAAGKSGAAGARSSRKRTNTMSLYDRRFQTTTEERRVANGYGGNAARSVTGSSSNEIESQEPQSHQYYGPNGGSNAAGAGAGLNGSAAALNHAPSMGTLCNIGNSCYLNSVVYTLRFAPHFLHNLHHLIQDLNVVQQTIVRQQTARSASLGKNVSAAQLEHARSWSSKDLATSTDQYSGQNGGVNSGNGGSGSSKSTHQSVTEKLHELYNNLHGNEMADSTEPYHADTLLHAIQDVNATFEGNQQQDAHEFLMCVLNCIRETNQSLIKAIGECPEVIANGYIANPDEVDTGEGQDRTDSTASQNLNAGNGSLATSQTTTTKTSFFSRKSKRKDEVKPSKSTRVQSPLKENSPTAGGITGAGTAHATANSLFYLNTVDLSGASSTSGSASTSASGVVSTSAALPTPPQATKYSSDDEMNSATVLKDKMRLEERIRELNLNFFSSDFEGIVVLTTKCLSCETITRQKQGMLDISVPVPISGYDNADLQDKPSTYIQNSCITKEYFRGENKYSCNQCTGYTEAIRSISYEVLPRLLVIQLNRFSGGMEKVSTYVPTTFTLPCFCATCCELGEGNKLHVYKLYSVITHVGATLTVGHYIAYTCFLDLASDYVNCPKDRRNTMTNSQTMASQAVPSNENAAPNNGSSSVASTPVIAAASALASSGNILMKKMKFGRSKASSSGDMSKNVKQVNGIISKNITNGIGKLSISTTCQGVNCCAMRLCCTQQTSSSNSTSCSDFSEESLQNGSNSNLSFGGSGSTYPTGYGSTGRGGVRANYAHGGPDPIWYMCDDDKIKAMTQREFEELLSPTRKITITPYLLFYARFDLQPQKATPPMPGSSTTPPPPSSAQSSWSNENVSSGSHKI

Target ORF information:

RefSeq Version	NM_143430.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 1, transcript variant D (Usp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143430.4

ORF Insert Sequence:

ATGCACGAAT ATGACGTAAA TGAGCGCAAA GAGGAACCAG CCAGTCTTCC ACCCACACAA 
AACGAGACCA CTGAAATCAA CAGCCAGCTA CGACAGGATC AGCAAAATCA GGAACAGGAG 
AATCCCCTAC GGCAGCAGGA TAACAGCCAC CCGCCTCAGG AAAATCCAAT TAATCAGCAG 
GAGAAGGACA CATTGCAAGT TTCCGTTCTT TATCCTCTGG TAGTCTGCGC GCAGCTGGCG 
GACAGTCAAC TGGTTCTGAC TGCGGACACG CCGAAGCTAT CTCCAGTTAA AAAGCGTCAG 
CGGCGCGTTT ATGTTGCGGA CACCTTGCGC TGCTACAGTC TACGTTCCCG GCAAGAGCCC 
GCGACCAAGG ACCCACTCGA AGGTGGCCTA AAGCAGCCGG ACGTCGTTCC ACCACCCCCG 
CCTGTCCAGG CGCCCTCTCC CACGCCCACA GAGTCCACAG AATACACACC TCCAGAACAA 
TTTAAATCGG AGTTTTGGAA CAGAATTGAA GAGGCTCAAG AGCCAAAGCC TGTTGGAAAG 
AAGCGACGAA CCCCTGGAGG ACTCAAAAGA ACGCGGGGCA ACAAGCTTCT AAAAGAGCTC 
GAGATCCAAT CGCCGGAAAC GGCGGCCGCT ATTGCGGCAG CACAGGCGGA ACTCAACGCC 
GCAGGAAAGT CGGGAGCAGC AGGAGCACGG TCCAGCCGCA AGAGGACCAA CACGATGTCT 
CTATACGATC GGCGATTCCA GACGACCACC GAGGAGCGCC GCGTGGCTAA CGGATACGGT 
GGCAATGCTG CGCGATCGGT CACTGGCTCC TCGTCAAACG AGATCGAGTC CCAGGAGCCG 
CAGAGTCATC AGTATTACGG TCCCAATGGC GGCAGCAATG CCGCGGGAGC AGGCGCAGGC 
TTGAACGGAA GTGCGGCGGC CCTGAACCAT GCCCCTTCCA TGGGCACACT GTGCAACATT 
GGCAACAGTT GCTACTTGAA TTCTGTGGTT TACACCCTGC GCTTTGCCCC GCACTTTTTG 
CACAACCTAC ACCACCTGAT ACAGGACTTG AATGTTGTGC AGCAGACGAT AGTTCGCCAA 
CAAACGGCCA GGTCTGCGTC CCTCGGCAAA AATGTGAGTG CCGCTCAGTT GGAGCACGCC 
CGCAGCTGGA GCAGCAAGGA TCTGGCCACC AGTACGGATC AGTACAGTGG CCAGAATGGC 
GGAGTTAATA GCGGAAACGG TGGTAGCGGC TCGAGCAAGT CGACTCATCA GTCGGTAACC 
GAAAAACTCC ATGAACTGTA TAACAACCTG CACGGCAACG AGATGGCAGA CTCCACGGAG 
CCCTACCACG CGGACACGCT GCTGCACGCC ATTCAGGACG TAAACGCCAC CTTTGAAGGT 
AACCAGCAGC AGGACGCACA CGAATTCCTA ATGTGCGTGC TTAATTGCAT CAGGGAAACG 
AATCAGTCTT TGATCAAGGC CATTGGCGAG TGTCCTGAGG TCATCGCAAA TGGATACATA 
GCCAACCCGG ATGAGGTCGA CACCGGAGAG GGACAGGATC GCACTGATTC GACTGCGTCG 
CAGAACCTGA ACGCTGGCAA TGGATCGCTG GCAACTAGTC AAACAACCAC GACCAAAACG 
TCGTTCTTCT CTCGCAAATC AAAAAGAAAA GACGAGGTCA AGCCATCCAA GTCGACGCGC 
GTTCAGTCGC CACTCAAGGA GAATAGTCCC ACGGCGGGCG GGATAACCGG TGCGGGCACA 
GCCCATGCCA CCGCCAACAG CCTGTTCTAC CTGAACACAG TTGATCTCAG CGGCGCCAGC 
AGCACCAGCG GTAGTGCCAG TACGAGTGCC AGTGGCGTTG TTTCCACAAG TGCAGCGTTG 
CCCACACCCC CGCAGGCTAC AAAATACTCC AGTGACGACG AGATGAATTC CGCCACCGTG 
CTGAAGGACA AAATGCGACT GGAGGAGCGA ATACGGGAGC TCAATCTAAA CTTCTTCAGC 
TCGGACTTCG AAGGCATTGT GGTGCTGACC ACCAAGTGCC TCAGTTGCGA GACGATAACG 
CGGCAAAAGC AGGGCATGCT TGATATTTCT GTGCCGGTGC CGATCTCGGG GTACGACAAT 
GCTGATCTCC AAGACAAACC GAGCACCTAC ATACAGAATT CCTGCATTAC GAAAGAGTAT 
TTCCGCGGGG AGAATAAGTA TAGTTGCAAC CAGTGCACCG GCTACACCGA AGCCATCCGC 
TCCATCTCGT ACGAGGTGCT GCCGCGGTTA CTGGTCATTC AGCTGAATCG CTTCTCTGGC 
GGCATGGAGA AAGTGAGTAC GTACGTGCCG ACCACCTTCA CTCTGCCCTG TTTCTGCGCC 
ACCTGCTGTG AGCTGGGCGA GGGCAACAAG CTGCACGTTT ACAAGCTGTA CAGCGTGATC 
ACGCATGTGG GAGCCACCCT GACGGTGGGC CACTACATCG CCTATACGTG CTTCTTGGAT 
CTCGCCAGTG ACTATGTCAA CTGCCCTAAG GATCGCAGGA ATACGATGAC GAACTCGCAA 
ACGATGGCTT CGCAAGCAGT GCCCTCCAAT GAGAATGCTG CGCCAAACAA TGGTAGCAGC 
TCCGTGGCCA GCACTCCAGT TATTGCAGCA GCTTCGGCAT TGGCCTCATC GGGCAACATT 
TTGATGAAGA AGATGAAGTT CGGTCGCAGC AAGGCCTCCA GCAGTGGTGA TATGAGCAAG 
AACGTTAAGC AGGTTAATGG GATAATCAGC AAGAACATCA CCAATGGAAT TGGCAAGCTG 
AGCATAAGCA CCACCTGCCA AGGAGTCAAC TGCTGTGCCA TGAGGCTGTG TTGCACCCAG 
CAGACGAGCA GCAGCAACTC GACCAGTTGC AGTGATTTCA GCGAGGAGTC CCTGCAAAAC 
GGAAGCAACA GCAATCTCAG CTTCGGAGGA TCGGGCAGCA CCTATCCCAC GGGCTACGGA 
AGCACGGGTC GCGGTGGTGT CAGAGCCAAC TACGCCCACG GAGGTCCTGA TCCCATTTGG 
TATATGTGCG ACGACGACAA GATCAAGGCG ATGACCCAAA GGGAGTTCGA AGAGCTGCTA 
TCGCCCACAC GGAAGATCAC AATAACGCCG TATTTGCTCT TTTACGCGCG ATTCGATCTG 
CAGCCGCAGA AGGCGACGCC GCCGATGCCT GGATCATCCA CAACGCCTCC GCCACCGTCG 
TCGGCACAGA GTTCGTGGTC GAATGAAAAC GTGTCCAGCG GCTCGCACAA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp1 ( NM_001276132.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263061.1 Drosophila melanogaster ubiquitin specific protease 1, transcript variant E (Usp1), mRNA.

Price & Availability↑

CloneID	OFa06485
Clone ID Related Accession (Same CDS sequence)	NM_143430.4 , NM_001276132.1 , NM_170403.3
Accession Version	NM_001276132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAT ATGACGTAAA TGAGCGCAAA GAGGAACCAG CCAGTCTTCC ACCCACACAA 
AACGAGACCA CTGAAATCAA CAGCCAGCTA CGACAGGATC AGCAAAATCA GGAACAGGAG 
AATCCCCTAC GGCAGCAGGA TAACAGCCAC CCGCCTCAGG AAAATCCAAT TAATCAGCAG 
GAGAAGGACA CATTGCAAGT TTCCGTTCTT TATCCTCTGG TAGTCTGCGC GCAGCTGGCG 
GACAGTCAAC TGGTTCTGAC TGCGGACACG CCGAAGCTAT CTCCAGTTAA AAAGCGTCAG 
CGGCGCGTTT ATGTTGCGGA CACCTTGCGC TGCTACAGTC TACGTTCCCG GCAAGAGCCC 
GCGACCAAGG ACCCACTCGA AGGTGGCCTA AAGCAGCCGG ACGTCGTTCC ACCACCCCCG 
CCTGTCCAGG CGCCCTCTCC CACGCCCACA GAGTCCACAG AATACACACC TCCAGAACAA 
TTTAAATCGG AGTTTTGGAA CAGAATTGAA GAGGCTCAAG AGCCAAAGCC TGTTGGAAAG 
AAGCGACGAA CCCCTGGAGG ACTCAAAAGA ACGCGGGGCA ACAAGCTTCT AAAAGAGCTC 
GAGATCCAAT CGCCGGAAAC GGCGGCCGCT ATTGCGGCAG CACAGGCGGA ACTCAACGCC 
GCAGGAAAGT CGGGAGCAGC AGGAGCACGG TCCAGCCGCA AGAGGACCAA CACGATGTCT 
CTATACGATC GGCGATTCCA GACGACCACC GAGGAGCGCC GCGTGGCTAA CGGATACGGT 
GGCAATGCTG CGCGATCGGT CACTGGCTCC TCGTCAAACG AGATCGAGTC CCAGGAGCCG 
CAGAGTCATC AGTATTACGG TCCCAATGGC GGCAGCAATG CCGCGGGAGC AGGCGCAGGC 
TTGAACGGAA GTGCGGCGGC CCTGAACCAT GCCCCTTCCA TGGGCACACT GTGCAACATT 
GGCAACAGTT GCTACTTGAA TTCTGTGGTT TACACCCTGC GCTTTGCCCC GCACTTTTTG 
CACAACCTAC ACCACCTGAT ACAGGACTTG AATGTTGTGC AGCAGACGAT AGTTCGCCAA 
CAAACGGCCA GGTCTGCGTC CCTCGGCAAA AATGTGAGTG CCGCTCAGTT GGAGCACGCC 
CGCAGCTGGA GCAGCAAGGA TCTGGCCACC AGTACGGATC AGTACAGTGG CCAGAATGGC 
GGAGTTAATA GCGGAAACGG TGGTAGCGGC TCGAGCAAGT CGACTCATCA GTCGGTAACC 
GAAAAACTCC ATGAACTGTA TAACAACCTG CACGGCAACG AGATGGCAGA CTCCACGGAG 
CCCTACCACG CGGACACGCT GCTGCACGCC ATTCAGGACG TAAACGCCAC CTTTGAAGGT 
AACCAGCAGC AGGACGCACA CGAATTCCTA ATGTGCGTGC TTAATTGCAT CAGGGAAACG 
AATCAGTCTT TGATCAAGGC CATTGGCGAG TGTCCTGAGG TCATCGCAAA TGGATACATA 
GCCAACCCGG ATGAGGTCGA CACCGGAGAG GGACAGGATC GCACTGATTC GACTGCGTCG 
CAGAACCTGA ACGCTGGCAA TGGATCGCTG GCAACTAGTC AAACAACCAC GACCAAAACG 
TCGTTCTTCT CTCGCAAATC AAAAAGAAAA GACGAGGTCA AGCCATCCAA GTCGACGCGC 
GTTCAGTCGC CACTCAAGGA GAATAGTCCC ACGGCGGGCG GGATAACCGG TGCGGGCACA 
GCCCATGCCA CCGCCAACAG CCTGTTCTAC CTGAACACAG TTGATCTCAG CGGCGCCAGC 
AGCACCAGCG GTAGTGCCAG TACGAGTGCC AGTGGCGTTG TTTCCACAAG TGCAGCGTTG 
CCCACACCCC CGCAGGCTAC AAAATACTCC AGTGACGACG AGATGAATTC CGCCACCGTG 
CTGAAGGACA AAATGCGACT GGAGGAGCGA ATACGGGAGC TCAATCTAAA CTTCTTCAGC 
TCGGACTTCG AAGGCATTGT GGTGCTGACC ACCAAGTGCC TCAGTTGCGA GACGATAACG 
CGGCAAAAGC AGGGCATGCT TGATATTTCT GTGCCGGTGC CGATCTCGGG GTACGACAAT 
GCTGATCTCC AAGACAAACC GAGCACCTAC ATACAGAATT CCTGCATTAC GAAAGAGTAT 
TTCCGCGGGG AGAATAAGTA TAGTTGCAAC CAGTGCACCG GCTACACCGA AGCCATCCGC 
TCCATCTCGT ACGAGGTGCT GCCGCGGTTA CTGGTCATTC AGCTGAATCG CTTCTCTGGC 
GGCATGGAGA AAGTGAGTAC GTACGTGCCG ACCACCTTCA CTCTGCCCTG TTTCTGCGCC 
ACCTGCTGTG AGCTGGGCGA GGGCAACAAG CTGCACGTTT ACAAGCTGTA CAGCGTGATC 
ACGCATGTGG GAGCCACCCT GACGGTGGGC CACTACATCG CCTATACGTG CTTCTTGGAT 
CTCGCCAGTG ACTATGTCAA CTGCCCTAAG GATCGCAGGA ATACGATGAC GAACTCGCAA 
ACGATGGCTT CGCAAGCAGT GCCCTCCAAT GAGAATGCTG CGCCAAACAA TGGTAGCAGC 
TCCGTGGCCA GCACTCCAGT TATTGCAGCA GCTTCGGCAT TGGCCTCATC GGGCAACATT 
TTGATGAAGA AGATGAAGTT CGGTCGCAGC AAGGCCTCCA GCAGTGGTGA TATGAGCAAG 
AACGTTAAGC AGGTTAATGG GATAATCAGC AAGAACATCA CCAATGGAAT TGGCAAGCTG 
AGCATAAGCA CCACCTGCCA AGGAGTCAAC TGCTGTGCCA TGAGGCTGTG TTGCACCCAG 
CAGACGAGCA GCAGCAACTC GACCAGTTGC AGTGATTTCA GCGAGGAGTC CCTGCAAAAC 
GGAAGCAACA GCAATCTCAG CTTCGGAGGA TCGGGCAGCA CCTATCCCAC GGGCTACGGA 
AGCACGGGTC GCGGTGGTGT CAGAGCCAAC TACGCCCACG GAGGTCCTGA TCCCATTTGG 
TATATGTGCG ACGACGACAA GATCAAGGCG ATGACCCAAA GGGAGTTCGA AGAGCTGCTA 
TCGCCCACAC GGAAGATCAC AATAACGCCG TATTTGCTCT TTTACGCGCG ATTCGATCTG 
CAGCCGCAGA AGGCGACGCC GCCGATGCCT GGATCATCCA CAACGCCTCC GCCACCGTCG 
TCGGCACAGA GTTCGTGGTC GAATGAAAAC GTGTCCAGCG GCTCGCACAA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263061.1
CDS	269..3505
Translation	MHEYDVNERKEEPASLPPTQNETTEINSQLRQDQQNQEQENPLRQQDNSHPPQENPINQQEKDTLQVSVLYPLVVCAQLADSQLVLTADTPKLSPVKKRQRRVYVADTLRCYSLRSRQEPATKDPLEGGLKQPDVVPPPPPVQAPSPTPTESTEYTPPEQFKSEFWNRIEEAQEPKPVGKKRRTPGGLKRTRGNKLLKELEIQSPETAAAIAAAQAELNAAGKSGAAGARSSRKRTNTMSLYDRRFQTTTEERRVANGYGGNAARSVTGSSSNEIESQEPQSHQYYGPNGGSNAAGAGAGLNGSAAALNHAPSMGTLCNIGNSCYLNSVVYTLRFAPHFLHNLHHLIQDLNVVQQTIVRQQTARSASLGKNVSAAQLEHARSWSSKDLATSTDQYSGQNGGVNSGNGGSGSSKSTHQSVTEKLHELYNNLHGNEMADSTEPYHADTLLHAIQDVNATFEGNQQQDAHEFLMCVLNCIRETNQSLIKAIGECPEVIANGYIANPDEVDTGEGQDRTDSTASQNLNAGNGSLATSQTTTTKTSFFSRKSKRKDEVKPSKSTRVQSPLKENSPTAGGITGAGTAHATANSLFYLNTVDLSGASSTSGSASTSASGVVSTSAALPTPPQATKYSSDDEMNSATVLKDKMRLEERIRELNLNFFSSDFEGIVVLTTKCLSCETITRQKQGMLDISVPVPISGYDNADLQDKPSTYIQNSCITKEYFRGENKYSCNQCTGYTEAIRSISYEVLPRLLVIQLNRFSGGMEKVSTYVPTTFTLPCFCATCCELGEGNKLHVYKLYSVITHVGATLTVGHYIAYTCFLDLASDYVNCPKDRRNTMTNSQTMASQAVPSNENAAPNNGSSSVASTPVIAAASALASSGNILMKKMKFGRSKASSSGDMSKNVKQVNGIISKNITNGIGKLSISTTCQGVNCCAMRLCCTQQTSSSNSTSCSDFSEESLQNGSNSNLSFGGSGSTYPTGYGSTGRGGVRANYAHGGPDPIWYMCDDDKIKAMTQREFEELLSPTRKITITPYLLFYARFDLQPQKATPPMPGSSTTPPPPSSAQSSWSNENVSSGSHKI

Target ORF information:

RefSeq Version	NM_001276132.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 1, transcript variant E (Usp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276132.1

ORF Insert Sequence:

ATGCACGAAT ATGACGTAAA TGAGCGCAAA GAGGAACCAG CCAGTCTTCC ACCCACACAA 
AACGAGACCA CTGAAATCAA CAGCCAGCTA CGACAGGATC AGCAAAATCA GGAACAGGAG 
AATCCCCTAC GGCAGCAGGA TAACAGCCAC CCGCCTCAGG AAAATCCAAT TAATCAGCAG 
GAGAAGGACA CATTGCAAGT TTCCGTTCTT TATCCTCTGG TAGTCTGCGC GCAGCTGGCG 
GACAGTCAAC TGGTTCTGAC TGCGGACACG CCGAAGCTAT CTCCAGTTAA AAAGCGTCAG 
CGGCGCGTTT ATGTTGCGGA CACCTTGCGC TGCTACAGTC TACGTTCCCG GCAAGAGCCC 
GCGACCAAGG ACCCACTCGA AGGTGGCCTA AAGCAGCCGG ACGTCGTTCC ACCACCCCCG 
CCTGTCCAGG CGCCCTCTCC CACGCCCACA GAGTCCACAG AATACACACC TCCAGAACAA 
TTTAAATCGG AGTTTTGGAA CAGAATTGAA GAGGCTCAAG AGCCAAAGCC TGTTGGAAAG 
AAGCGACGAA CCCCTGGAGG ACTCAAAAGA ACGCGGGGCA ACAAGCTTCT AAAAGAGCTC 
GAGATCCAAT CGCCGGAAAC GGCGGCCGCT ATTGCGGCAG CACAGGCGGA ACTCAACGCC 
GCAGGAAAGT CGGGAGCAGC AGGAGCACGG TCCAGCCGCA AGAGGACCAA CACGATGTCT 
CTATACGATC GGCGATTCCA GACGACCACC GAGGAGCGCC GCGTGGCTAA CGGATACGGT 
GGCAATGCTG CGCGATCGGT CACTGGCTCC TCGTCAAACG AGATCGAGTC CCAGGAGCCG 
CAGAGTCATC AGTATTACGG TCCCAATGGC GGCAGCAATG CCGCGGGAGC AGGCGCAGGC 
TTGAACGGAA GTGCGGCGGC CCTGAACCAT GCCCCTTCCA TGGGCACACT GTGCAACATT 
GGCAACAGTT GCTACTTGAA TTCTGTGGTT TACACCCTGC GCTTTGCCCC GCACTTTTTG 
CACAACCTAC ACCACCTGAT ACAGGACTTG AATGTTGTGC AGCAGACGAT AGTTCGCCAA 
CAAACGGCCA GGTCTGCGTC CCTCGGCAAA AATGTGAGTG CCGCTCAGTT GGAGCACGCC 
CGCAGCTGGA GCAGCAAGGA TCTGGCCACC AGTACGGATC AGTACAGTGG CCAGAATGGC 
GGAGTTAATA GCGGAAACGG TGGTAGCGGC TCGAGCAAGT CGACTCATCA GTCGGTAACC 
GAAAAACTCC ATGAACTGTA TAACAACCTG CACGGCAACG AGATGGCAGA CTCCACGGAG 
CCCTACCACG CGGACACGCT GCTGCACGCC ATTCAGGACG TAAACGCCAC CTTTGAAGGT 
AACCAGCAGC AGGACGCACA CGAATTCCTA ATGTGCGTGC TTAATTGCAT CAGGGAAACG 
AATCAGTCTT TGATCAAGGC CATTGGCGAG TGTCCTGAGG TCATCGCAAA TGGATACATA 
GCCAACCCGG ATGAGGTCGA CACCGGAGAG GGACAGGATC GCACTGATTC GACTGCGTCG 
CAGAACCTGA ACGCTGGCAA TGGATCGCTG GCAACTAGTC AAACAACCAC GACCAAAACG 
TCGTTCTTCT CTCGCAAATC AAAAAGAAAA GACGAGGTCA AGCCATCCAA GTCGACGCGC 
GTTCAGTCGC CACTCAAGGA GAATAGTCCC ACGGCGGGCG GGATAACCGG TGCGGGCACA 
GCCCATGCCA CCGCCAACAG CCTGTTCTAC CTGAACACAG TTGATCTCAG CGGCGCCAGC 
AGCACCAGCG GTAGTGCCAG TACGAGTGCC AGTGGCGTTG TTTCCACAAG TGCAGCGTTG 
CCCACACCCC CGCAGGCTAC AAAATACTCC AGTGACGACG AGATGAATTC CGCCACCGTG 
CTGAAGGACA AAATGCGACT GGAGGAGCGA ATACGGGAGC TCAATCTAAA CTTCTTCAGC 
TCGGACTTCG AAGGCATTGT GGTGCTGACC ACCAAGTGCC TCAGTTGCGA GACGATAACG 
CGGCAAAAGC AGGGCATGCT TGATATTTCT GTGCCGGTGC CGATCTCGGG GTACGACAAT 
GCTGATCTCC AAGACAAACC GAGCACCTAC ATACAGAATT CCTGCATTAC GAAAGAGTAT 
TTCCGCGGGG AGAATAAGTA TAGTTGCAAC CAGTGCACCG GCTACACCGA AGCCATCCGC 
TCCATCTCGT ACGAGGTGCT GCCGCGGTTA CTGGTCATTC AGCTGAATCG CTTCTCTGGC 
GGCATGGAGA AAGTGAGTAC GTACGTGCCG ACCACCTTCA CTCTGCCCTG TTTCTGCGCC 
ACCTGCTGTG AGCTGGGCGA GGGCAACAAG CTGCACGTTT ACAAGCTGTA CAGCGTGATC 
ACGCATGTGG GAGCCACCCT GACGGTGGGC CACTACATCG CCTATACGTG CTTCTTGGAT 
CTCGCCAGTG ACTATGTCAA CTGCCCTAAG GATCGCAGGA ATACGATGAC GAACTCGCAA 
ACGATGGCTT CGCAAGCAGT GCCCTCCAAT GAGAATGCTG CGCCAAACAA TGGTAGCAGC 
TCCGTGGCCA GCACTCCAGT TATTGCAGCA GCTTCGGCAT TGGCCTCATC GGGCAACATT 
TTGATGAAGA AGATGAAGTT CGGTCGCAGC AAGGCCTCCA GCAGTGGTGA TATGAGCAAG 
AACGTTAAGC AGGTTAATGG GATAATCAGC AAGAACATCA CCAATGGAAT TGGCAAGCTG 
AGCATAAGCA CCACCTGCCA AGGAGTCAAC TGCTGTGCCA TGAGGCTGTG TTGCACCCAG 
CAGACGAGCA GCAGCAACTC GACCAGTTGC AGTGATTTCA GCGAGGAGTC CCTGCAAAAC 
GGAAGCAACA GCAATCTCAG CTTCGGAGGA TCGGGCAGCA CCTATCCCAC GGGCTACGGA 
AGCACGGGTC GCGGTGGTGT CAGAGCCAAC TACGCCCACG GAGGTCCTGA TCCCATTTGG 
TATATGTGCG ACGACGACAA GATCAAGGCG ATGACCCAAA GGGAGTTCGA AGAGCTGCTA 
TCGCCCACAC GGAAGATCAC AATAACGCCG TATTTGCTCT TTTACGCGCG ATTCGATCTG 
CAGCCGCAGA AGGCGACGCC GCCGATGCCT GGATCATCCA CAACGCCTCC GCCACCGTCG 
TCGGCACAGA GTTCGTGGTC GAATGAAAAC GTGTCCAGCG GCTCGCACAA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp1 ( NM_170403.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733282.1 Drosophila melanogaster ubiquitin specific protease 1, transcript variant C (Usp1), mRNA.

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CloneID	OFa06485
Clone ID Related Accession (Same CDS sequence)	NM_143430.4 , NM_001276132.1 , NM_170403.3
Accession Version	NM_170403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170403.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAT ATGACGTAAA TGAGCGCAAA GAGGAACCAG CCAGTCTTCC ACCCACACAA 
AACGAGACCA CTGAAATCAA CAGCCAGCTA CGACAGGATC AGCAAAATCA GGAACAGGAG 
AATCCCCTAC GGCAGCAGGA TAACAGCCAC CCGCCTCAGG AAAATCCAAT TAATCAGCAG 
GAGAAGGACA CATTGCAAGT TTCCGTTCTT TATCCTCTGG TAGTCTGCGC GCAGCTGGCG 
GACAGTCAAC TGGTTCTGAC TGCGGACACG CCGAAGCTAT CTCCAGTTAA AAAGCGTCAG 
CGGCGCGTTT ATGTTGCGGA CACCTTGCGC TGCTACAGTC TACGTTCCCG GCAAGAGCCC 
GCGACCAAGG ACCCACTCGA AGGTGGCCTA AAGCAGCCGG ACGTCGTTCC ACCACCCCCG 
CCTGTCCAGG CGCCCTCTCC CACGCCCACA GAGTCCACAG AATACACACC TCCAGAACAA 
TTTAAATCGG AGTTTTGGAA CAGAATTGAA GAGGCTCAAG AGCCAAAGCC TGTTGGAAAG 
AAGCGACGAA CCCCTGGAGG ACTCAAAAGA ACGCGGGGCA ACAAGCTTCT AAAAGAGCTC 
GAGATCCAAT CGCCGGAAAC GGCGGCCGCT ATTGCGGCAG CACAGGCGGA ACTCAACGCC 
GCAGGAAAGT CGGGAGCAGC AGGAGCACGG TCCAGCCGCA AGAGGACCAA CACGATGTCT 
CTATACGATC GGCGATTCCA GACGACCACC GAGGAGCGCC GCGTGGCTAA CGGATACGGT 
GGCAATGCTG CGCGATCGGT CACTGGCTCC TCGTCAAACG AGATCGAGTC CCAGGAGCCG 
CAGAGTCATC AGTATTACGG TCCCAATGGC GGCAGCAATG CCGCGGGAGC AGGCGCAGGC 
TTGAACGGAA GTGCGGCGGC CCTGAACCAT GCCCCTTCCA TGGGCACACT GTGCAACATT 
GGCAACAGTT GCTACTTGAA TTCTGTGGTT TACACCCTGC GCTTTGCCCC GCACTTTTTG 
CACAACCTAC ACCACCTGAT ACAGGACTTG AATGTTGTGC AGCAGACGAT AGTTCGCCAA 
CAAACGGCCA GGTCTGCGTC CCTCGGCAAA AATGTGAGTG CCGCTCAGTT GGAGCACGCC 
CGCAGCTGGA GCAGCAAGGA TCTGGCCACC AGTACGGATC AGTACAGTGG CCAGAATGGC 
GGAGTTAATA GCGGAAACGG TGGTAGCGGC TCGAGCAAGT CGACTCATCA GTCGGTAACC 
GAAAAACTCC ATGAACTGTA TAACAACCTG CACGGCAACG AGATGGCAGA CTCCACGGAG 
CCCTACCACG CGGACACGCT GCTGCACGCC ATTCAGGACG TAAACGCCAC CTTTGAAGGT 
AACCAGCAGC AGGACGCACA CGAATTCCTA ATGTGCGTGC TTAATTGCAT CAGGGAAACG 
AATCAGTCTT TGATCAAGGC CATTGGCGAG TGTCCTGAGG TCATCGCAAA TGGATACATA 
GCCAACCCGG ATGAGGTCGA CACCGGAGAG GGACAGGATC GCACTGATTC GACTGCGTCG 
CAGAACCTGA ACGCTGGCAA TGGATCGCTG GCAACTAGTC AAACAACCAC GACCAAAACG 
TCGTTCTTCT CTCGCAAATC AAAAAGAAAA GACGAGGTCA AGCCATCCAA GTCGACGCGC 
GTTCAGTCGC CACTCAAGGA GAATAGTCCC ACGGCGGGCG GGATAACCGG TGCGGGCACA 
GCCCATGCCA CCGCCAACAG CCTGTTCTAC CTGAACACAG TTGATCTCAG CGGCGCCAGC 
AGCACCAGCG GTAGTGCCAG TACGAGTGCC AGTGGCGTTG TTTCCACAAG TGCAGCGTTG 
CCCACACCCC CGCAGGCTAC AAAATACTCC AGTGACGACG AGATGAATTC CGCCACCGTG 
CTGAAGGACA AAATGCGACT GGAGGAGCGA ATACGGGAGC TCAATCTAAA CTTCTTCAGC 
TCGGACTTCG AAGGCATTGT GGTGCTGACC ACCAAGTGCC TCAGTTGCGA GACGATAACG 
CGGCAAAAGC AGGGCATGCT TGATATTTCT GTGCCGGTGC CGATCTCGGG GTACGACAAT 
GCTGATCTCC AAGACAAACC GAGCACCTAC ATACAGAATT CCTGCATTAC GAAAGAGTAT 
TTCCGCGGGG AGAATAAGTA TAGTTGCAAC CAGTGCACCG GCTACACCGA AGCCATCCGC 
TCCATCTCGT ACGAGGTGCT GCCGCGGTTA CTGGTCATTC AGCTGAATCG CTTCTCTGGC 
GGCATGGAGA AAGTGAGTAC GTACGTGCCG ACCACCTTCA CTCTGCCCTG TTTCTGCGCC 
ACCTGCTGTG AGCTGGGCGA GGGCAACAAG CTGCACGTTT ACAAGCTGTA CAGCGTGATC 
ACGCATGTGG GAGCCACCCT GACGGTGGGC CACTACATCG CCTATACGTG CTTCTTGGAT 
CTCGCCAGTG ACTATGTCAA CTGCCCTAAG GATCGCAGGA ATACGATGAC GAACTCGCAA 
ACGATGGCTT CGCAAGCAGT GCCCTCCAAT GAGAATGCTG CGCCAAACAA TGGTAGCAGC 
TCCGTGGCCA GCACTCCAGT TATTGCAGCA GCTTCGGCAT TGGCCTCATC GGGCAACATT 
TTGATGAAGA AGATGAAGTT CGGTCGCAGC AAGGCCTCCA GCAGTGGTGA TATGAGCAAG 
AACGTTAAGC AGGTTAATGG GATAATCAGC AAGAACATCA CCAATGGAAT TGGCAAGCTG 
AGCATAAGCA CCACCTGCCA AGGAGTCAAC TGCTGTGCCA TGAGGCTGTG TTGCACCCAG 
CAGACGAGCA GCAGCAACTC GACCAGTTGC AGTGATTTCA GCGAGGAGTC CCTGCAAAAC 
GGAAGCAACA GCAATCTCAG CTTCGGAGGA TCGGGCAGCA CCTATCCCAC GGGCTACGGA 
AGCACGGGTC GCGGTGGTGT CAGAGCCAAC TACGCCCACG GAGGTCCTGA TCCCATTTGG 
TATATGTGCG ACGACGACAA GATCAAGGCG ATGACCCAAA GGGAGTTCGA AGAGCTGCTA 
TCGCCCACAC GGAAGATCAC AATAACGCCG TATTTGCTCT TTTACGCGCG ATTCGATCTG 
CAGCCGCAGA AGGCGACGCC GCCGATGCCT GGATCATCCA CAACGCCTCC GCCACCGTCG 
TCGGCACAGA GTTCGTGGTC GAATGAAAAC GTGTCCAGCG GCTCGCACAA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733282.1
CDS	269..3505
Translation	MHEYDVNERKEEPASLPPTQNETTEINSQLRQDQQNQEQENPLRQQDNSHPPQENPINQQEKDTLQVSVLYPLVVCAQLADSQLVLTADTPKLSPVKKRQRRVYVADTLRCYSLRSRQEPATKDPLEGGLKQPDVVPPPPPVQAPSPTPTESTEYTPPEQFKSEFWNRIEEAQEPKPVGKKRRTPGGLKRTRGNKLLKELEIQSPETAAAIAAAQAELNAAGKSGAAGARSSRKRTNTMSLYDRRFQTTTEERRVANGYGGNAARSVTGSSSNEIESQEPQSHQYYGPNGGSNAAGAGAGLNGSAAALNHAPSMGTLCNIGNSCYLNSVVYTLRFAPHFLHNLHHLIQDLNVVQQTIVRQQTARSASLGKNVSAAQLEHARSWSSKDLATSTDQYSGQNGGVNSGNGGSGSSKSTHQSVTEKLHELYNNLHGNEMADSTEPYHADTLLHAIQDVNATFEGNQQQDAHEFLMCVLNCIRETNQSLIKAIGECPEVIANGYIANPDEVDTGEGQDRTDSTASQNLNAGNGSLATSQTTTTKTSFFSRKSKRKDEVKPSKSTRVQSPLKENSPTAGGITGAGTAHATANSLFYLNTVDLSGASSTSGSASTSASGVVSTSAALPTPPQATKYSSDDEMNSATVLKDKMRLEERIRELNLNFFSSDFEGIVVLTTKCLSCETITRQKQGMLDISVPVPISGYDNADLQDKPSTYIQNSCITKEYFRGENKYSCNQCTGYTEAIRSISYEVLPRLLVIQLNRFSGGMEKVSTYVPTTFTLPCFCATCCELGEGNKLHVYKLYSVITHVGATLTVGHYIAYTCFLDLASDYVNCPKDRRNTMTNSQTMASQAVPSNENAAPNNGSSSVASTPVIAAASALASSGNILMKKMKFGRSKASSSGDMSKNVKQVNGIISKNITNGIGKLSISTTCQGVNCCAMRLCCTQQTSSSNSTSCSDFSEESLQNGSNSNLSFGGSGSTYPTGYGSTGRGGVRANYAHGGPDPIWYMCDDDKIKAMTQREFEELLSPTRKITITPYLLFYARFDLQPQKATPPMPGSSTTPPPPSSAQSSWSNENVSSGSHKI

Target ORF information:

RefSeq Version	NM_170403.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 1, transcript variant C (Usp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170403.3

ORF Insert Sequence:

ATGCACGAAT ATGACGTAAA TGAGCGCAAA GAGGAACCAG CCAGTCTTCC ACCCACACAA 
AACGAGACCA CTGAAATCAA CAGCCAGCTA CGACAGGATC AGCAAAATCA GGAACAGGAG 
AATCCCCTAC GGCAGCAGGA TAACAGCCAC CCGCCTCAGG AAAATCCAAT TAATCAGCAG 
GAGAAGGACA CATTGCAAGT TTCCGTTCTT TATCCTCTGG TAGTCTGCGC GCAGCTGGCG 
GACAGTCAAC TGGTTCTGAC TGCGGACACG CCGAAGCTAT CTCCAGTTAA AAAGCGTCAG 
CGGCGCGTTT ATGTTGCGGA CACCTTGCGC TGCTACAGTC TACGTTCCCG GCAAGAGCCC 
GCGACCAAGG ACCCACTCGA AGGTGGCCTA AAGCAGCCGG ACGTCGTTCC ACCACCCCCG 
CCTGTCCAGG CGCCCTCTCC CACGCCCACA GAGTCCACAG AATACACACC TCCAGAACAA 
TTTAAATCGG AGTTTTGGAA CAGAATTGAA GAGGCTCAAG AGCCAAAGCC TGTTGGAAAG 
AAGCGACGAA CCCCTGGAGG ACTCAAAAGA ACGCGGGGCA ACAAGCTTCT AAAAGAGCTC 
GAGATCCAAT CGCCGGAAAC GGCGGCCGCT ATTGCGGCAG CACAGGCGGA ACTCAACGCC 
GCAGGAAAGT CGGGAGCAGC AGGAGCACGG TCCAGCCGCA AGAGGACCAA CACGATGTCT 
CTATACGATC GGCGATTCCA GACGACCACC GAGGAGCGCC GCGTGGCTAA CGGATACGGT 
GGCAATGCTG CGCGATCGGT CACTGGCTCC TCGTCAAACG AGATCGAGTC CCAGGAGCCG 
CAGAGTCATC AGTATTACGG TCCCAATGGC GGCAGCAATG CCGCGGGAGC AGGCGCAGGC 
TTGAACGGAA GTGCGGCGGC CCTGAACCAT GCCCCTTCCA TGGGCACACT GTGCAACATT 
GGCAACAGTT GCTACTTGAA TTCTGTGGTT TACACCCTGC GCTTTGCCCC GCACTTTTTG 
CACAACCTAC ACCACCTGAT ACAGGACTTG AATGTTGTGC AGCAGACGAT AGTTCGCCAA 
CAAACGGCCA GGTCTGCGTC CCTCGGCAAA AATGTGAGTG CCGCTCAGTT GGAGCACGCC 
CGCAGCTGGA GCAGCAAGGA TCTGGCCACC AGTACGGATC AGTACAGTGG CCAGAATGGC 
GGAGTTAATA GCGGAAACGG TGGTAGCGGC TCGAGCAAGT CGACTCATCA GTCGGTAACC 
GAAAAACTCC ATGAACTGTA TAACAACCTG CACGGCAACG AGATGGCAGA CTCCACGGAG 
CCCTACCACG CGGACACGCT GCTGCACGCC ATTCAGGACG TAAACGCCAC CTTTGAAGGT 
AACCAGCAGC AGGACGCACA CGAATTCCTA ATGTGCGTGC TTAATTGCAT CAGGGAAACG 
AATCAGTCTT TGATCAAGGC CATTGGCGAG TGTCCTGAGG TCATCGCAAA TGGATACATA 
GCCAACCCGG ATGAGGTCGA CACCGGAGAG GGACAGGATC GCACTGATTC GACTGCGTCG 
CAGAACCTGA ACGCTGGCAA TGGATCGCTG GCAACTAGTC AAACAACCAC GACCAAAACG 
TCGTTCTTCT CTCGCAAATC AAAAAGAAAA GACGAGGTCA AGCCATCCAA GTCGACGCGC 
GTTCAGTCGC CACTCAAGGA GAATAGTCCC ACGGCGGGCG GGATAACCGG TGCGGGCACA 
GCCCATGCCA CCGCCAACAG CCTGTTCTAC CTGAACACAG TTGATCTCAG CGGCGCCAGC 
AGCACCAGCG GTAGTGCCAG TACGAGTGCC AGTGGCGTTG TTTCCACAAG TGCAGCGTTG 
CCCACACCCC CGCAGGCTAC AAAATACTCC AGTGACGACG AGATGAATTC CGCCACCGTG 
CTGAAGGACA AAATGCGACT GGAGGAGCGA ATACGGGAGC TCAATCTAAA CTTCTTCAGC 
TCGGACTTCG AAGGCATTGT GGTGCTGACC ACCAAGTGCC TCAGTTGCGA GACGATAACG 
CGGCAAAAGC AGGGCATGCT TGATATTTCT GTGCCGGTGC CGATCTCGGG GTACGACAAT 
GCTGATCTCC AAGACAAACC GAGCACCTAC ATACAGAATT CCTGCATTAC GAAAGAGTAT 
TTCCGCGGGG AGAATAAGTA TAGTTGCAAC CAGTGCACCG GCTACACCGA AGCCATCCGC 
TCCATCTCGT ACGAGGTGCT GCCGCGGTTA CTGGTCATTC AGCTGAATCG CTTCTCTGGC 
GGCATGGAGA AAGTGAGTAC GTACGTGCCG ACCACCTTCA CTCTGCCCTG TTTCTGCGCC 
ACCTGCTGTG AGCTGGGCGA GGGCAACAAG CTGCACGTTT ACAAGCTGTA CAGCGTGATC 
ACGCATGTGG GAGCCACCCT GACGGTGGGC CACTACATCG CCTATACGTG CTTCTTGGAT 
CTCGCCAGTG ACTATGTCAA CTGCCCTAAG GATCGCAGGA ATACGATGAC GAACTCGCAA 
ACGATGGCTT CGCAAGCAGT GCCCTCCAAT GAGAATGCTG CGCCAAACAA TGGTAGCAGC 
TCCGTGGCCA GCACTCCAGT TATTGCAGCA GCTTCGGCAT TGGCCTCATC GGGCAACATT 
TTGATGAAGA AGATGAAGTT CGGTCGCAGC AAGGCCTCCA GCAGTGGTGA TATGAGCAAG 
AACGTTAAGC AGGTTAATGG GATAATCAGC AAGAACATCA CCAATGGAAT TGGCAAGCTG 
AGCATAAGCA CCACCTGCCA AGGAGTCAAC TGCTGTGCCA TGAGGCTGTG TTGCACCCAG 
CAGACGAGCA GCAGCAACTC GACCAGTTGC AGTGATTTCA GCGAGGAGTC CCTGCAAAAC 
GGAAGCAACA GCAATCTCAG CTTCGGAGGA TCGGGCAGCA CCTATCCCAC GGGCTACGGA 
AGCACGGGTC GCGGTGGTGT CAGAGCCAAC TACGCCCACG GAGGTCCTGA TCCCATTTGG 
TATATGTGCG ACGACGACAA GATCAAGGCG ATGACCCAAA GGGAGTTCGA AGAGCTGCTA 
TCGCCCACAC GGAAGATCAC AATAACGCCG TATTTGCTCT TTTACGCGCG ATTCGATCTG 
CAGCCGCAGA AGGCGACGCC GCCGATGCCT GGATCATCCA CAACGCCTCC GCCACCGTCG 
TCGGCACAGA GTTCGTGGTC GAATGAAAAC GTGTCCAGCG GCTCGCACAA AATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Usp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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