CG12290 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12290
Entrez Gene ID	43182
Full Name	CG12290 gene product from transcript CG12290-RB
Synonyms	Dmel\CG12290,anon-WO0170980.49,anon-WO0170980.50,lincRNA.S7704
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97A6-97A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_143213.4	NP_651470.1	uncharacterized protein, isoform A
NM_001300611.1	NP_001287540.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG12290	NP_651470.1
Anopheles gambiae (African malaria mosquito)	GPRHIS	XP_312371.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12290 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12290 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06196	NM_143213.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12290), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa06196	NM_001300611.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12290), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12290 ( NM_143213.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651470.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12290), mRNA.

Price & Availability↑

CloneID	OFa06196
Clone ID Related Accession (Same CDS sequence)	NM_143213.4 , NM_001300611.1
Accession Version	NM_143213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143213.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATAG ACCTCCTGAT CCTGGCCCTG CTGCTCGTCT CGTTCCTGAT CAACCTGCTG 
GCGCTGTGTG CCTTTTGGAT AACGCCTGGC TTGAGGACCA CCGCCAATCG CTTTACGATC 
AACCTGCTGG CCATCAATCT CATTGGTTGC TGCATCTTGG CCCCCACTTT GTTCCTGGGA 
CTGCCGGGAA AGTCTGCGGA GGCATCCACT TCCAACGCCG AGACCCTGGA GTTCTTCTCG 
AAGCCGGGAA ACCATCAGGT GATACTTCGT CGCAATGGCC AGCTGGTGGA ACAGGACGGC 
GTGGTGGTGC GAAGGAACAT CAGCGAAAAC GGCGATACGG TGGAGACCTT CTTCAAGTGC 
AATGCCACCT ACTGTCGCGA GTTGACCATC GATGAGCGCG GAGATGGTGG CTTTGTCATC 
ACGGAAACCG AGACACACGA AGAGAATCTC TCGGCCTTTG AGAGCTTACC CACGGAGGCT 
CCCATTTTGC CGCCCGTCCA ACTGAGATGT TGGTCCATAG ACATGACTGC TGCGCTGGGA 
GCTCTGGCAG TACTGCTCGT GGTGGGGGAC ACCTGGTGTG CCGTTACGGA TCCCCTGCGC 
TACCACAGTC GCATTTCGGG CGTGAAAACC TGGATCTTCA TAGCACTCAC CTGGGTGGTG 
GGCATACTCT TTGGAGCCCT TTCCGCCTTC CGAGTTTTGG ACTTTGAGGC GGACGCCCTG 
TTCAGCAGAC AACGCCGCCT GGCAGTCACG TACTTCAACA TCAGCAGCAC CAATAGTATT 
TTCGGAGTGG TGTACGCCAG TGTCTACTTC ATCGTGATCA TCCTCCTGCC CTTCGGCTTC 
GTGTGCGGAA TGTACTGGAG GATATTCAGT GAGGCGCGCG GAAATGGGCT GCGCATGCGC 
CAGAATGGCT CTTCGCCGCT GCTGCAGAGC GCCTTGAATC TGACGGCTGG CCAGCAGGCG 
GCTCAAGCCA ACCAGTTCTC CAATAGCTTG TGTGTTCATC GGCATTCCAT ATCCTCGGCC 
AGTTCGCATG GCGGCAACAG CAGCTTGGGC TTGGGCGGTC TGCAAATGCA AATTGATCAG 
CGCCAACAGC CGAGGAGTTC GCCCAGTTGT CTCCGTCGGG ACTCGGCCGC GAAAGTACTT 
TTGCCCACCA TCTCGGATGA TGGTGGCTCA GATGCGGAAA GTGGAGCCGG AGTTCAACTG 
ATGCCCGTAC AGGAGCACTC ACTGTCAGAT AGAAATCAGA ACATTATGCT TACTCTGCAA 
ACAGCCAGTG GTGAAATAAA GAGGAACTAC TCCGCCAGAC AGCTGCCCCT GTTGGGCACT 
TCTTCGCAGG ATCTGAGGGA GACTAATCGT CTGCAGGGCA TACGCCAGGT GCACAGTTCG 
CCCAATCTCC ACAAGTACAC GGAACTGCGC CAGGACTCGC TCAGTGAGGA GTGTGGTTCT 
CCGCATCTTT TGGGTCACGC CCAGCGGCAG CAGCAGCAGC AATTGCACCT GCAACATCAG 
CAGCAACAGC ATCATCACCA CCAGCAACAT CATCCGCACT TCAGTTCGCC GCGCCACCAG 
CAACACGGCC ACGCCCTCCA GATACCCGCC ATTCATGCCT CCCCCAAGGC TCTGAGTTAC 
ATGAGCTCCT TGCGACACCG TTTGAGCAAC GCCAGTTCGC TATTCAAATA CCGTGAGGAG 
TCGAGAGCAG CCCGCATCAG CATCCTGGTG GTGGTGATGT TTGTGGTCTC GTACCTGCCC 
TTCGGACTGC TGGTACTCCT GCAATCGCGA CTGTCGGCTG CTAACTTTGG CGGATCCTCC 
CAGCTGGCCA TCTTCATGAT CCTGCTGGCC AATCTCAGTT CGCCATTCAT CTTCGCCTAC 
AGGAACAAGA GGGTGAGGCG AGGAGTCAAG CGGCTGTTTG GTCTGGATTC GTCGTCGGGC 
CTGCAGCGCA ACTGCAGCAG CAGTGTAAAG ACCAATGGCA CCGCAGGTCC TGCTGCAAGT 
GGTGCACAAT TGCAGCGCAA CAGCAGCAAG TTGTCGCAGT ACAGCAGCAA CAGCTGCAAG 
TATCTCACCC CCCAGAGTTC CCTGGTCAGT CAAGTGCCGG TGCATACGAC CTTGACGTTA 
AGGCCCAACA GCAGCTGCAG CACCATCATC AATTTCGGTG GCGCCAGGGG CTCAGCGGAC 
TCCGATGAGC AGCCACCGGC AACACCACCA CCTACTGTGG CAGTTGCTCC ACCGCCCACA 
CGACCGCAGC GCCCCAAGCT GCAGAGGGGC ATCACCATCG TTGAGCACAT AGCTATAACA 
CCAACCATGC CCCAAAAGTT CCAGAATCGG CGCGCCAGGC TCTTCGACAT GTTCTTCCGC 
AGCTCCAAGA AACTGCAGGC GGGCTGCCAG AGCCAGAGTT TGCCCACTGA GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651470.1
CDS	458..2854
Translation	MAIDLLILALLLVSFLINLLALCAFWITPGLRTTANRFTINLLAINLIGCCILAPTLFLGLPGKSAEASTSNAETLEFFSKPGNHQVILRRNGQLVEQDGVVVRRNISENGDTVETFFKCNATYCRELTIDERGDGGFVITETETHEENLSAFESLPTEAPILPPVQLRCWSIDMTAALGALAVLLVVGDTWCAVTDPLRYHSRISGVKTWIFIALTWVVGILFGALSAFRVLDFEADALFSRQRRLAVTYFNISSTNSIFGVVYASVYFIVIILLPFGFVCGMYWRIFSEARGNGLRMRQNGSSPLLQSALNLTAGQQAAQANQFSNSLCVHRHSISSASSHGGNSSLGLGGLQMQIDQRQQPRSSPSCLRRDSAAKVLLPTISDDGGSDAESGAGVQLMPVQEHSLSDRNQNIMLTLQTASGEIKRNYSARQLPLLGTSSQDLRETNRLQGIRQVHSSPNLHKYTELRQDSLSEECGSPHLLGHAQRQQQQQLHLQHQQQQHHHHQQHHPHFSSPRHQQHGHALQIPAIHASPKALSYMSSLRHRLSNASSLFKYREESRAARISILVVVMFVVSYLPFGLLVLLQSRLSAANFGGSSQLAIFMILLANLSSPFIFAYRNKRVRRGVKRLFGLDSSSGLQRNCSSSVKTNGTAGPAASGAQLQRNSSKLSQYSSNSCKYLTPQSSLVSQVPVHTTLTLRPNSSCSTIINFGGARGSADSDEQPPATPPPTVAVAPPPTRPQRPKLQRGITIVEHIAITPTMPQKFQNRRARLFDMFFRSSKKLQAGCQSQSLPTEV

Target ORF information:

RefSeq Version	NM_143213.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12290), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143213.4

ORF Insert Sequence:

ATGGCCATAG ACCTCCTGAT CCTGGCCCTG CTGCTCGTCT CGTTCCTGAT CAACCTGCTG 
GCGCTGTGTG CCTTTTGGAT AACGCCTGGC TTGAGGACCA CCGCCAATCG CTTTACGATC 
AACCTGCTGG CCATCAATCT CATTGGTTGC TGCATCTTGG CCCCCACTTT GTTCCTGGGA 
CTGCCGGGAA AGTCTGCGGA GGCATCCACT TCCAACGCCG AGACCCTGGA GTTCTTCTCG 
AAGCCGGGAA ACCATCAGGT GATACTTCGT CGCAATGGCC AGCTGGTGGA ACAGGACGGC 
GTGGTGGTGC GAAGGAACAT CAGCGAAAAC GGCGATACGG TGGAGACCTT CTTCAAGTGC 
AATGCCACCT ACTGTCGCGA GTTGACCATC GATGAGCGCG GAGATGGTGG CTTTGTCATC 
ACGGAAACCG AGACACACGA AGAGAATCTC TCGGCCTTTG AGAGCTTACC CACGGAGGCT 
CCCATTTTGC CGCCCGTCCA ACTGAGATGT TGGTCCATAG ACATGACTGC TGCGCTGGGA 
GCTCTGGCAG TACTGCTCGT GGTGGGGGAC ACCTGGTGTG CCGTTACGGA TCCCCTGCGC 
TACCACAGTC GCATTTCGGG CGTGAAAACC TGGATCTTCA TAGCACTCAC CTGGGTGGTG 
GGCATACTCT TTGGAGCCCT TTCCGCCTTC CGAGTTTTGG ACTTTGAGGC GGACGCCCTG 
TTCAGCAGAC AACGCCGCCT GGCAGTCACG TACTTCAACA TCAGCAGCAC CAATAGTATT 
TTCGGAGTGG TGTACGCCAG TGTCTACTTC ATCGTGATCA TCCTCCTGCC CTTCGGCTTC 
GTGTGCGGAA TGTACTGGAG GATATTCAGT GAGGCGCGCG GAAATGGGCT GCGCATGCGC 
CAGAATGGCT CTTCGCCGCT GCTGCAGAGC GCCTTGAATC TGACGGCTGG CCAGCAGGCG 
GCTCAAGCCA ACCAGTTCTC CAATAGCTTG TGTGTTCATC GGCATTCCAT ATCCTCGGCC 
AGTTCGCATG GCGGCAACAG CAGCTTGGGC TTGGGCGGTC TGCAAATGCA AATTGATCAG 
CGCCAACAGC CGAGGAGTTC GCCCAGTTGT CTCCGTCGGG ACTCGGCCGC GAAAGTACTT 
TTGCCCACCA TCTCGGATGA TGGTGGCTCA GATGCGGAAA GTGGAGCCGG AGTTCAACTG 
ATGCCCGTAC AGGAGCACTC ACTGTCAGAT AGAAATCAGA ACATTATGCT TACTCTGCAA 
ACAGCCAGTG GTGAAATAAA GAGGAACTAC TCCGCCAGAC AGCTGCCCCT GTTGGGCACT 
TCTTCGCAGG ATCTGAGGGA GACTAATCGT CTGCAGGGCA TACGCCAGGT GCACAGTTCG 
CCCAATCTCC ACAAGTACAC GGAACTGCGC CAGGACTCGC TCAGTGAGGA GTGTGGTTCT 
CCGCATCTTT TGGGTCACGC CCAGCGGCAG CAGCAGCAGC AATTGCACCT GCAACATCAG 
CAGCAACAGC ATCATCACCA CCAGCAACAT CATCCGCACT TCAGTTCGCC GCGCCACCAG 
CAACACGGCC ACGCCCTCCA GATACCCGCC ATTCATGCCT CCCCCAAGGC TCTGAGTTAC 
ATGAGCTCCT TGCGACACCG TTTGAGCAAC GCCAGTTCGC TATTCAAATA CCGTGAGGAG 
TCGAGAGCAG CCCGCATCAG CATCCTGGTG GTGGTGATGT TTGTGGTCTC GTACCTGCCC 
TTCGGACTGC TGGTACTCCT GCAATCGCGA CTGTCGGCTG CTAACTTTGG CGGATCCTCC 
CAGCTGGCCA TCTTCATGAT CCTGCTGGCC AATCTCAGTT CGCCATTCAT CTTCGCCTAC 
AGGAACAAGA GGGTGAGGCG AGGAGTCAAG CGGCTGTTTG GTCTGGATTC GTCGTCGGGC 
CTGCAGCGCA ACTGCAGCAG CAGTGTAAAG ACCAATGGCA CCGCAGGTCC TGCTGCAAGT 
GGTGCACAAT TGCAGCGCAA CAGCAGCAAG TTGTCGCAGT ACAGCAGCAA CAGCTGCAAG 
TATCTCACCC CCCAGAGTTC CCTGGTCAGT CAAGTGCCGG TGCATACGAC CTTGACGTTA 
AGGCCCAACA GCAGCTGCAG CACCATCATC AATTTCGGTG GCGCCAGGGG CTCAGCGGAC 
TCCGATGAGC AGCCACCGGC AACACCACCA CCTACTGTGG CAGTTGCTCC ACCGCCCACA 
CGACCGCAGC GCCCCAAGCT GCAGAGGGGC ATCACCATCG TTGAGCACAT AGCTATAACA 
CCAACCATGC CCCAAAAGTT CCAGAATCGG CGCGCCAGGC TCTTCGACAT GTTCTTCCGC 
AGCTCCAAGA AACTGCAGGC GGGCTGCCAG AGCCAGAGTT TGCCCACTGA GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12290 ( NM_001300611.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287540.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12290), mRNA.

Price & Availability↑

CloneID	OFa06196
Clone ID Related Accession (Same CDS sequence)	NM_143213.4 , NM_001300611.1
Accession Version	NM_001300611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATAG ACCTCCTGAT CCTGGCCCTG CTGCTCGTCT CGTTCCTGAT CAACCTGCTG 
GCGCTGTGTG CCTTTTGGAT AACGCCTGGC TTGAGGACCA CCGCCAATCG CTTTACGATC 
AACCTGCTGG CCATCAATCT CATTGGTTGC TGCATCTTGG CCCCCACTTT GTTCCTGGGA 
CTGCCGGGAA AGTCTGCGGA GGCATCCACT TCCAACGCCG AGACCCTGGA GTTCTTCTCG 
AAGCCGGGAA ACCATCAGGT GATACTTCGT CGCAATGGCC AGCTGGTGGA ACAGGACGGC 
GTGGTGGTGC GAAGGAACAT CAGCGAAAAC GGCGATACGG TGGAGACCTT CTTCAAGTGC 
AATGCCACCT ACTGTCGCGA GTTGACCATC GATGAGCGCG GAGATGGTGG CTTTGTCATC 
ACGGAAACCG AGACACACGA AGAGAATCTC TCGGCCTTTG AGAGCTTACC CACGGAGGCT 
CCCATTTTGC CGCCCGTCCA ACTGAGATGT TGGTCCATAG ACATGACTGC TGCGCTGGGA 
GCTCTGGCAG TACTGCTCGT GGTGGGGGAC ACCTGGTGTG CCGTTACGGA TCCCCTGCGC 
TACCACAGTC GCATTTCGGG CGTGAAAACC TGGATCTTCA TAGCACTCAC CTGGGTGGTG 
GGCATACTCT TTGGAGCCCT TTCCGCCTTC CGAGTTTTGG ACTTTGAGGC GGACGCCCTG 
TTCAGCAGAC AACGCCGCCT GGCAGTCACG TACTTCAACA TCAGCAGCAC CAATAGTATT 
TTCGGAGTGG TGTACGCCAG TGTCTACTTC ATCGTGATCA TCCTCCTGCC CTTCGGCTTC 
GTGTGCGGAA TGTACTGGAG GATATTCAGT GAGGCGCGCG GAAATGGGCT GCGCATGCGC 
CAGAATGGCT CTTCGCCGCT GCTGCAGAGC GCCTTGAATC TGACGGCTGG CCAGCAGGCG 
GCTCAAGCCA ACCAGTTCTC CAATAGCTTG TGTGTTCATC GGCATTCCAT ATCCTCGGCC 
AGTTCGCATG GCGGCAACAG CAGCTTGGGC TTGGGCGGTC TGCAAATGCA AATTGATCAG 
CGCCAACAGC CGAGGAGTTC GCCCAGTTGT CTCCGTCGGG ACTCGGCCGC GAAAGTACTT 
TTGCCCACCA TCTCGGATGA TGGTGGCTCA GATGCGGAAA GTGGAGCCGG AGTTCAACTG 
ATGCCCGTAC AGGAGCACTC ACTGTCAGAT AGAAATCAGA ACATTATGCT TACTCTGCAA 
ACAGCCAGTG GTGAAATAAA GAGGAACTAC TCCGCCAGAC AGCTGCCCCT GTTGGGCACT 
TCTTCGCAGG ATCTGAGGGA GACTAATCGT CTGCAGGGCA TACGCCAGGT GCACAGTTCG 
CCCAATCTCC ACAAGTACAC GGAACTGCGC CAGGACTCGC TCAGTGAGGA GTGTGGTTCT 
CCGCATCTTT TGGGTCACGC CCAGCGGCAG CAGCAGCAGC AATTGCACCT GCAACATCAG 
CAGCAACAGC ATCATCACCA CCAGCAACAT CATCCGCACT TCAGTTCGCC GCGCCACCAG 
CAACACGGCC ACGCCCTCCA GATACCCGCC ATTCATGCCT CCCCCAAGGC TCTGAGTTAC 
ATGAGCTCCT TGCGACACCG TTTGAGCAAC GCCAGTTCGC TATTCAAATA CCGTGAGGAG 
TCGAGAGCAG CCCGCATCAG CATCCTGGTG GTGGTGATGT TTGTGGTCTC GTACCTGCCC 
TTCGGACTGC TGGTACTCCT GCAATCGCGA CTGTCGGCTG CTAACTTTGG CGGATCCTCC 
CAGCTGGCCA TCTTCATGAT CCTGCTGGCC AATCTCAGTT CGCCATTCAT CTTCGCCTAC 
AGGAACAAGA GGGTGAGGCG AGGAGTCAAG CGGCTGTTTG GTCTGGATTC GTCGTCGGGC 
CTGCAGCGCA ACTGCAGCAG CAGTGTAAAG ACCAATGGCA CCGCAGGTCC TGCTGCAAGT 
GGTGCACAAT TGCAGCGCAA CAGCAGCAAG TTGTCGCAGT ACAGCAGCAA CAGCTGCAAG 
TATCTCACCC CCCAGAGTTC CCTGGTCAGT CAAGTGCCGG TGCATACGAC CTTGACGTTA 
AGGCCCAACA GCAGCTGCAG CACCATCATC AATTTCGGTG GCGCCAGGGG CTCAGCGGAC 
TCCGATGAGC AGCCACCGGC AACACCACCA CCTACTGTGG CAGTTGCTCC ACCGCCCACA 
CGACCGCAGC GCCCCAAGCT GCAGAGGGGC ATCACCATCG TTGAGCACAT AGCTATAACA 
CCAACCATGC CCCAAAAGTT CCAGAATCGG CGCGCCAGGC TCTTCGACAT GTTCTTCCGC 
AGCTCCAAGA AACTGCAGGC GGGCTGCCAG AGCCAGAGTT TGCCCACTGA GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287540.1
CDS	458..2854
Translation	MAIDLLILALLLVSFLINLLALCAFWITPGLRTTANRFTINLLAINLIGCCILAPTLFLGLPGKSAEASTSNAETLEFFSKPGNHQVILRRNGQLVEQDGVVVRRNISENGDTVETFFKCNATYCRELTIDERGDGGFVITETETHEENLSAFESLPTEAPILPPVQLRCWSIDMTAALGALAVLLVVGDTWCAVTDPLRYHSRISGVKTWIFIALTWVVGILFGALSAFRVLDFEADALFSRQRRLAVTYFNISSTNSIFGVVYASVYFIVIILLPFGFVCGMYWRIFSEARGNGLRMRQNGSSPLLQSALNLTAGQQAAQANQFSNSLCVHRHSISSASSHGGNSSLGLGGLQMQIDQRQQPRSSPSCLRRDSAAKVLLPTISDDGGSDAESGAGVQLMPVQEHSLSDRNQNIMLTLQTASGEIKRNYSARQLPLLGTSSQDLRETNRLQGIRQVHSSPNLHKYTELRQDSLSEECGSPHLLGHAQRQQQQQLHLQHQQQQHHHHQQHHPHFSSPRHQQHGHALQIPAIHASPKALSYMSSLRHRLSNASSLFKYREESRAARISILVVVMFVVSYLPFGLLVLLQSRLSAANFGGSSQLAIFMILLANLSSPFIFAYRNKRVRRGVKRLFGLDSSSGLQRNCSSSVKTNGTAGPAASGAQLQRNSSKLSQYSSNSCKYLTPQSSLVSQVPVHTTLTLRPNSSCSTIINFGGARGSADSDEQPPATPPPTVAVAPPPTRPQRPKLQRGITIVEHIAITPTMPQKFQNRRARLFDMFFRSSKKLQAGCQSQSLPTEV

Target ORF information:

RefSeq Version	NM_001300611.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12290), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300611.1

ORF Insert Sequence:

ATGGCCATAG ACCTCCTGAT CCTGGCCCTG CTGCTCGTCT CGTTCCTGAT CAACCTGCTG 
GCGCTGTGTG CCTTTTGGAT AACGCCTGGC TTGAGGACCA CCGCCAATCG CTTTACGATC 
AACCTGCTGG CCATCAATCT CATTGGTTGC TGCATCTTGG CCCCCACTTT GTTCCTGGGA 
CTGCCGGGAA AGTCTGCGGA GGCATCCACT TCCAACGCCG AGACCCTGGA GTTCTTCTCG 
AAGCCGGGAA ACCATCAGGT GATACTTCGT CGCAATGGCC AGCTGGTGGA ACAGGACGGC 
GTGGTGGTGC GAAGGAACAT CAGCGAAAAC GGCGATACGG TGGAGACCTT CTTCAAGTGC 
AATGCCACCT ACTGTCGCGA GTTGACCATC GATGAGCGCG GAGATGGTGG CTTTGTCATC 
ACGGAAACCG AGACACACGA AGAGAATCTC TCGGCCTTTG AGAGCTTACC CACGGAGGCT 
CCCATTTTGC CGCCCGTCCA ACTGAGATGT TGGTCCATAG ACATGACTGC TGCGCTGGGA 
GCTCTGGCAG TACTGCTCGT GGTGGGGGAC ACCTGGTGTG CCGTTACGGA TCCCCTGCGC 
TACCACAGTC GCATTTCGGG CGTGAAAACC TGGATCTTCA TAGCACTCAC CTGGGTGGTG 
GGCATACTCT TTGGAGCCCT TTCCGCCTTC CGAGTTTTGG ACTTTGAGGC GGACGCCCTG 
TTCAGCAGAC AACGCCGCCT GGCAGTCACG TACTTCAACA TCAGCAGCAC CAATAGTATT 
TTCGGAGTGG TGTACGCCAG TGTCTACTTC ATCGTGATCA TCCTCCTGCC CTTCGGCTTC 
GTGTGCGGAA TGTACTGGAG GATATTCAGT GAGGCGCGCG GAAATGGGCT GCGCATGCGC 
CAGAATGGCT CTTCGCCGCT GCTGCAGAGC GCCTTGAATC TGACGGCTGG CCAGCAGGCG 
GCTCAAGCCA ACCAGTTCTC CAATAGCTTG TGTGTTCATC GGCATTCCAT ATCCTCGGCC 
AGTTCGCATG GCGGCAACAG CAGCTTGGGC TTGGGCGGTC TGCAAATGCA AATTGATCAG 
CGCCAACAGC CGAGGAGTTC GCCCAGTTGT CTCCGTCGGG ACTCGGCCGC GAAAGTACTT 
TTGCCCACCA TCTCGGATGA TGGTGGCTCA GATGCGGAAA GTGGAGCCGG AGTTCAACTG 
ATGCCCGTAC AGGAGCACTC ACTGTCAGAT AGAAATCAGA ACATTATGCT TACTCTGCAA 
ACAGCCAGTG GTGAAATAAA GAGGAACTAC TCCGCCAGAC AGCTGCCCCT GTTGGGCACT 
TCTTCGCAGG ATCTGAGGGA GACTAATCGT CTGCAGGGCA TACGCCAGGT GCACAGTTCG 
CCCAATCTCC ACAAGTACAC GGAACTGCGC CAGGACTCGC TCAGTGAGGA GTGTGGTTCT 
CCGCATCTTT TGGGTCACGC CCAGCGGCAG CAGCAGCAGC AATTGCACCT GCAACATCAG 
CAGCAACAGC ATCATCACCA CCAGCAACAT CATCCGCACT TCAGTTCGCC GCGCCACCAG 
CAACACGGCC ACGCCCTCCA GATACCCGCC ATTCATGCCT CCCCCAAGGC TCTGAGTTAC 
ATGAGCTCCT TGCGACACCG TTTGAGCAAC GCCAGTTCGC TATTCAAATA CCGTGAGGAG 
TCGAGAGCAG CCCGCATCAG CATCCTGGTG GTGGTGATGT TTGTGGTCTC GTACCTGCCC 
TTCGGACTGC TGGTACTCCT GCAATCGCGA CTGTCGGCTG CTAACTTTGG CGGATCCTCC 
CAGCTGGCCA TCTTCATGAT CCTGCTGGCC AATCTCAGTT CGCCATTCAT CTTCGCCTAC 
AGGAACAAGA GGGTGAGGCG AGGAGTCAAG CGGCTGTTTG GTCTGGATTC GTCGTCGGGC 
CTGCAGCGCA ACTGCAGCAG CAGTGTAAAG ACCAATGGCA CCGCAGGTCC TGCTGCAAGT 
GGTGCACAAT TGCAGCGCAA CAGCAGCAAG TTGTCGCAGT ACAGCAGCAA CAGCTGCAAG 
TATCTCACCC CCCAGAGTTC CCTGGTCAGT CAAGTGCCGG TGCATACGAC CTTGACGTTA 
AGGCCCAACA GCAGCTGCAG CACCATCATC AATTTCGGTG GCGCCAGGGG CTCAGCGGAC 
TCCGATGAGC AGCCACCGGC AACACCACCA CCTACTGTGG CAGTTGCTCC ACCGCCCACA 
CGACCGCAGC GCCCCAAGCT GCAGAGGGGC ATCACCATCG TTGAGCACAT AGCTATAACA 
CCAACCATGC CCCAAAAGTT CCAGAATCGG CGCGCCAGGC TCTTCGACAT GTTCTTCCGC 
AGCTCCAAGA AACTGCAGGC GGGCTGCCAG AGCCAGAGTT TGCCCACTGA GGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12290 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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