E(spl)mbeta-HLH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E(spl)mbeta-HLH
Entrez Gene ID	43152
Full Name	Enhancer of split mbeta, helix-loop-helix
Synonyms	CG14548,Dm-mA,Dmel\CG14548,E(Spl) HLH-Mbeta,E(Spl)mbeta,E(spl),E(spl) mbeta,E(spl)-Mbeta,E(spl)-mbeta,E(spl)Mbeta,E(spl)beta,E(spl)mA,E(spl)mb,E(spl)mbeta,E(spl)mbeta},HLH-mbeta,HLHMbeta,HLHmA,HLHmbeta,Mbeta,bHLHb34,m-beta,mA,mb,mbeta
General protein information	Preferred Names Enhancer of split mbeta, helix-loop-helix Names CG14548 gene product from transcript CG14548-RA CG14548-PA E(spl) region transcript mbeta E(spl)mbeta-HLH-PA enhancer of split m-beta enhancer of split mbeta enhancer of split mbeta, helix-loop-helix enhancer of split region transcript HLHmA split locus enhancer protein mA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96F9-96F9\|3-89.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079781.3	NP_524505.2	enhancer of split mbeta, helix-loop-helix

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	E(spl)mgamma-HLH	NP_524504.2
Drosophila melanogaster (fruit fly)	E(spl)mbeta-HLH	NP_524505.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012342	XP_320210.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The proliferation and differentiation of intestinal stem cells are not regulated by individual Notch downstream target E(spl)mbeta, but by different downstream targets collectively.
Title: Notch signaling downstream target E(spl)mbeta is dispensable for adult midgut homeostasis in Drosophila.

The following E(spl)mbeta-HLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E(spl)mbeta-HLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06165	NM_079781.3 Latest version!	Drosophila melanogaster enhancer of split mbeta, helix-loop-helix (E(spl)mbeta-HLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E(spl)mbeta-HLH ( NM_079781.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524505.2 Drosophila melanogaster enhancer of split mbeta, helix-loop-helix (E(spl)mbeta-HLH), mRNA.

Price & Availability↑

CloneID	OFa06165
Clone ID Related Accession (Same CDS sequence)	NM_079781.3
Accession Version	NM_079781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of split mbeta, helix-loop-helix
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079781.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCTGG AAATGGAGAT GTCCAAGACC TATCAGTACC GCAAGGTGAT GAAGCCCATG 
CTGGAGCGTA AGCGCCGTGC CAGGATTAAC AAGTGCCTGG ACGAGCTCAA GGACATCATG 
GTGGAGTGCC TGACCCAGGA GGGCGAGCAC ATCACCCGGC TGGAGAAGGC CGACATCCTG 
GAGCTGACCG TGGAGCACAT GAAGAAGCTG CGTGCCCAGA AGCAGCTGCG TCTGTCCAGC 
GTCACCGGTG GAGTGTCTCC GAGTGCCGAT CCCAAGCTTA GCATCGCTGA GAGTTTCCGT 
GCTGGCTACG TTCATGCTGC CAATGAAGTG TCCAAAACCT TGGCCGCTGT ACCCGGAGTG 
AGTGTGGATC TCGGCACCCA GCTGATGAGT CACCTTGGCC ATCGTCTCAA CTACCTGCAA 
GTGGTGGTTC CCTCGCTGCC CATTGGAGTG CCTCTTCAAG CTCCAGTAGA GGATCAAGCT 
ATGGTCACTC CACCTCCCTC GGAATGCGAC AGCTTGGAGA GCGGAGCCTG CAGCCCGGCG 
CCCAGCGAGG CCAGCTCCAC CTCTGGCCCC ATGTGGCGTC CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524505.2
CDS	131..718
Translation	MVLEMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECLTQEGEHITRLEKADILELTVEHMKKLRAQKQLRLSSVTGGVSPSADPKLSIAESFRAGYVHAANEVSKTLAAVPGVSVDLGTQLMSHLGHRLNYLQVVVPSLPIGVPLQAPVEDQAMVTPPPSECDSLESGACSPAPSEASSTSGPMWRPW

Target ORF information:

RefSeq Version	NM_079781.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of split mbeta, helix-loop-helix (E(spl)mbeta-HLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079781.3

ORF Insert Sequence:

ATGGTTCTGG AAATGGAGAT GTCCAAGACC TATCAGTACC GCAAGGTGAT GAAGCCCATG 
CTGGAGCGTA AGCGCCGTGC CAGGATTAAC AAGTGCCTGG ACGAGCTCAA GGACATCATG 
GTGGAGTGCC TGACCCAGGA GGGCGAGCAC ATCACCCGGC TGGAGAAGGC CGACATCCTG 
GAGCTGACCG TGGAGCACAT GAAGAAGCTG CGTGCCCAGA AGCAGCTGCG TCTGTCCAGC 
GTCACCGGTG GAGTGTCTCC GAGTGCCGAT CCCAAGCTTA GCATCGCTGA GAGTTTCCGT 
GCTGGCTACG TTCATGCTGC CAATGAAGTG TCCAAAACCT TGGCCGCTGT ACCCGGAGTG 
AGTGTGGATC TCGGCACCCA GCTGATGAGT CACCTTGGCC ATCGTCTCAA CTACCTGCAA 
GTGGTGGTTC CCTCGCTGCC CATTGGAGTG CCTCTTCAAG CTCCAGTAGA GGATCAAGCT 
ATGGTCACTC CACCTCCCTC GGAATGCGAC AGCTTGGAGA GCGGAGCCTG CAGCCCGGCG 
CCCAGCGAGG CCAGCTCCAC CTCTGGCCCC ATGTGGCGTC CCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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E(spl)mbeta-HLH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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