E(spl)mgamma-HLH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E(spl)mgamma-HLH
Entrez Gene ID	43151
Full Name	Enhancer of split mgamma, helix-loop-helix
Synonyms	CG8333,Dm-mB,Dmel\CG8333,E(Spl) HLH-m gamma,E(spl) m gamma,E(spl) mg,E(spl) mgamma,E(spl)-mgamma,E(spl)Mgamma,E(spl)gamma,E(spl)mB,E(spl)mgamma,HLH-mgamma,HLHMgamma,HLHmB,HLHmgamma,HlHmg,Mgamma,anon-EST:Liang-1.49,bHLHb34,clone 1.49,e(spl)mgamma,gamma,mB,mg,mgamma
General protein information	Preferred Names Enhancer of split mgamma, helix-loop-helix Names CG8333 gene product from transcript CG8333-RA CG8333-PA E(spl) region transcript g E(spl) region transcript mgamma E(spl)mgamma-HLH-PA enhancer of split mgamma enhancer of split mgamma, helix-loop-helix enhancer of split region transcript HLHmB split locus enhancer protein mB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96F9-96F9\|3-89.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079780.3	NP_524504.2	enhancer of split mgamma, helix-loop-helix

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	E(spl)mgamma-HLH	NP_524504.2
Drosophila melanogaster (fruit fly)	E(spl)mbeta-HLH	NP_524505.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012342	XP_320210.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Sc C-terminal domain is required for E(spl) recruitment in an enhancer context-dependent fashion, suggesting that in some enhancers alternative interaction surfaces can be used to recruit E(spl) proteins
Title: Role of the Sc C terminus in transcriptional activation and E(spl) repressor recruitment.

Data show that E(spl) proteins m7 and mgamma are potent inhibitors of neural fate, even in the presence of excess Sc activity and even when their DNA-binding basic domain has been inactivated
Title: Two modes of recruitment of E(spl) repressors onto target genes.

The following E(spl)mgamma-HLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E(spl)mgamma-HLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06164	NM_079780.3 Latest version!	Drosophila melanogaster enhancer of split mgamma, helix-loop-helix (E(spl)mgamma-HLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E(spl)mgamma-HLH ( NM_079780.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524504.2 Drosophila melanogaster enhancer of split mgamma, helix-loop-helix (E(spl)mgamma-HLH), mRNA.

Price & Availability↑

CloneID	OFa06164
Clone ID Related Accession (Same CDS sequence)	NM_079780.3
Accession Version	NM_079780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of split mgamma, helix-loop-helix
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079780.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCGC TACAAATGTC CGAGATGTCC AAGACCTATC AGTACCGCAA GGTGATGAAG 
CCCATGCTGG AGAGGAAGCG TCGTGCCAGA ATCAACAAGT GCCTGGACGA GCTAAAGGAT 
CTTATGGTTG CCACCTTGGA GTCCGAGGGC GAGCACGTCA CCCGTTTGGA GAAAGCCGAT 
ATCCTGGAAC TTACCGTCAC CCATTTGCAA AAGATGAAGC AGCAGCGTCA GCACAAGCGG 
GCTTCCGGCG ATGAGAGCTT GACTCCGGCG GAGGGCTTCC GTTCCGGCTA CATCCATGCC 
GTCAATGAGG TCTCCCGTTC ACTCTCCCAG CTGCCCGGCA TGAATGTCAG CCTAGGCACT 
CAGCTAATGA CCCACCTGGG ACAGCGCCTC AATCAGATCC AGCCAGCAGA AAAGGAAGTC 
TTGCCGGTGA CCGCTCCACT CTCCGTGCAC ATCGCCAATC GGGATGCCTA CTCCGTGCCC 
ATTTCGCCAA TCTCCAGCTA CGCCGGCAGT CCCAACTCCA ATACCAGTTC GACCAGCCAT 
TCCCTGTTGA CCACCATCGA TGTGACCAAG ATGGAGGACG ACAGCGAGGA CGAGGAGAAC 
GTCTGGCGTC CCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524504.2
CDS	93..710
Translation	MSSLQMSEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATLESEGEHVTRLEKADILELTVTHLQKMKQQRQHKRASGDESLTPAEGFRSGYIHAVNEVSRSLSQLPGMNVSLGTQLMTHLGQRLNQIQPAEKEVLPVTAPLSVHIANRDAYSVPISPISSYAGSPNSNTSSTSHSLLTTIDVTKMEDDSEDEENVWRPW

Target ORF information:

RefSeq Version	NM_079780.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of split mgamma, helix-loop-helix (E(spl)mgamma-HLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079780.3

ORF Insert Sequence:

ATGTCGTCGC TACAAATGTC CGAGATGTCC AAGACCTATC AGTACCGCAA GGTGATGAAG 
CCCATGCTGG AGAGGAAGCG TCGTGCCAGA ATCAACAAGT GCCTGGACGA GCTAAAGGAT 
CTTATGGTTG CCACCTTGGA GTCCGAGGGC GAGCACGTCA CCCGTTTGGA GAAAGCCGAT 
ATCCTGGAAC TTACCGTCAC CCATTTGCAA AAGATGAAGC AGCAGCGTCA GCACAAGCGG 
GCTTCCGGCG ATGAGAGCTT GACTCCGGCG GAGGGCTTCC GTTCCGGCTA CATCCATGCC 
GTCAATGAGG TCTCCCGTTC ACTCTCCCAG CTGCCCGGCA TGAATGTCAG CCTAGGCACT 
CAGCTAATGA CCCACCTGGG ACAGCGCCTC AATCAGATCC AGCCAGCAGA AAAGGAAGTC 
TTGCCGGTGA CCGCTCCACT CTCCGTGCAC ATCGCCAATC GGGATGCCTA CTCCGTGCCC 
ATTTCGCCAA TCTCCAGCTA CGCCGGCAGT CCCAACTCCA ATACCAGTTC GACCAGCCAT 
TCCCTGTTGA CCACCATCGA TGTGACCAAG ATGGAGGACG ACAGCGAGGA CGAGGAGAAC 
GTCTGGCGTC CCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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E(spl)mgamma-HLH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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