CG14540 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG14540
Entrez Gene ID	43145
Full Name	CG14540 gene product from transcript CG14540-RA
Synonyms	Dmel\CG14540
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96F8-96F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_143194.2	NP_651451.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG14540 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG14540 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06157	NM_143194.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG14540), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG14540 ( NM_143194.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651451.1 Drosophila melanogaster uncharacterized protein (CG14540), mRNA.

Price & Availability↑

CloneID	OFa06157
Clone ID Related Accession (Same CDS sequence)	NM_143194.2
Accession Version	NM_143194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143194.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGATGG TGCGCCCAAT TCGTCGCAAG GGAATCGAGG AGGTCTATGG TTCCAAAATG 
GATTCCTTGC ATCCGGTAAA GTCGGTAGCG ATATCCCATG CAGGTGTGAC CAAGAAAACA 
GCAAGGGCTG AGAGATCGGG TCCAGGCGGT GCAACTGGCG GTGGAGGAAC CATTAGCTCC 
CGACAAGTCG ATGCTCGCAC TCAGCGCAGT TTTGTCACCG AAAATGATTA CTACATCACC 
ACCAATCTGC CGTTGACGGC GGCCAATCTG CGAAATGCCC CCGGTTCGAT GGCGCAGCCA 
TCAATATCGC AAATGATTCC CGGCCATGTC CAGCCATCTC CATATAACGC CATGCCCTCG 
AGGCGAAACC AAATGGCCAA TAAGGCCAGT GCTCGTCAGG CGCCGATGAA CAAGGTGCCG 
CATTTTAGCC CGGCCGAAGG CACGGTAAAC CCTAATGGCT ATGGCATGTC CAAGTCGAAG 
GAGACATACC GAATGAAGCA CCGCGAAAAG GGCAATACCC AGAGGAACGA CTATCCAATG 
GATGACAACG ACGACTACTC CGATAAGGAA CTAATGGTGC CCCAGCAGGG AAACATGGGA 
AATATGCGCC GGCACAATCC GCACTCTCAT GCCCATCAAA TGCAAATGCA GTTGCAATCC 
GGAGCAATGC AGCATCCGCC GCAACACCAG CTGCACCAGC AGCAGCAGAT GACTACTCAC 
AACCACCTCA AGCATCAGCA ACAGCAGCGC CTCCAGCATT CGCAACCGCA TCAGCATCAG 
CATCAGCATC CGCATCCGCA TCAGAAGCAG ATGCGTGGGC GACAGCCTGT TCCGGCGAGC 
AAGGTGCACG TGAGTACCGC CGATGACGAT GAAGATAAGG AGGAAGATCC TGAGGAGTTC 
TTTGACCTGA TCCGCCAGAC TGTCAAAACG GCCGTTGGCA ACACCATATC CGATGCTCTG 
GCAAAGAACT TTCGCGATCT GAGCCACAAA ATCGAAAGGT TCTCGGGCGA GTTGAAGCAG 
ACAAACGATA ACCTGGATAA GCTGCAGGAA CAAGTCATTA GCAAGGTAAT TTACTTCGGT 
GAGGAAAATT CGCGACACTT TCGGTACTTG TGCATGAAAT CCGAGTACGA TAAAATGTTT 
TACCAGCATC AATCGCTGAT GAATGGAAAG CCCACTCAGG AAATGCTGAG TCTTTCGAAG 
GCGAACTTAG AAGCAGCGGC CTCAGTCAGT CAGAATCTGA CACAGAAACC GTCGCATAGT 
TTAAAACAAC AACCAGGAAA AGTGATTAGG AATCAAAGCA AAATGCCTAT TAGCGCCAAA 
AAAGACCATA ACGAGTCCCT AATGAACTCC AGTGCCTATC GTAGCATCTG CAAAGCTCAG 
CAGCCAGTTC TTGCTGCACA AAAGTCGTCA TCGGAGCAAA GTTTAGTTGT AAAATCATCT 
GATGTGGGCG TGCAAGAAGT CCTGGATAAT ATCCAGCGCT ACTGCAATCA GGTGAAGGAT 
ATGAATGGTC AGATGTCGGC TGAACTGCCC AGTCTCGAGG ACATCTTGAT ACCCAAGAAA 
AATTCAAACG GTGGACTTTG TCAGCCAGCA GCCATGAGCT CTAGGACCCT CGCAGGTAGT 
CAGAAAAATA AAAAGGTTGA CGACACGGAG ATCGAAACGC CCAACGATAG TACGGATGAG 
ACCTACACCG AAGTGGATGA CTTCCAGCTC TCCTCGGACA TCTCGACCGG CAGCGAGGAC 
GATGACTGTG GCCTCAAGTA TCCAAGTGGA GCAACTACGA GCCGGCCCAA CCGCAAATTA 
AATCGAAGGG GAGCCAACCA AGGCGCCGGA GATGGTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651451.1
CDS	198..2039
Translation	MKMVRPIRRKGIEEVYGSKMDSLHPVKSVAISHAGVTKKTARAERSGPGGATGGGGTISSRQVDARTQRSFVTENDYYITTNLPLTAANLRNAPGSMAQPSISQMIPGHVQPSPYNAMPSRRNQMANKASARQAPMNKVPHFSPAEGTVNPNGYGMSKSKETYRMKHREKGNTQRNDYPMDDNDDYSDKELMVPQQGNMGNMRRHNPHSHAHQMQMQLQSGAMQHPPQHQLHQQQQMTTHNHLKHQQQQRLQHSQPHQHQHQHPHPHQKQMRGRQPVPASKVHVSTADDDEDKEEDPEEFFDLIRQTVKTAVGNTISDALAKNFRDLSHKIERFSGELKQTNDNLDKLQEQVISKVIYFGEENSRHFRYLCMKSEYDKMFYQHQSLMNGKPTQEMLSLSKANLEAAASVSQNLTQKPSHSLKQQPGKVIRNQSKMPISAKKDHNESLMNSSAYRSICKAQQPVLAAQKSSSEQSLVVKSSDVGVQEVLDNIQRYCNQVKDMNGQMSAELPSLEDILIPKKNSNGGLCQPAAMSSRTLAGSQKNKKVDDTEIETPNDSTDETYTEVDDFQLSSDISTGSEDDDCGLKYPSGATTSRPNRKLNRRGANQGAGDGQ

Target ORF information:

RefSeq Version	NM_143194.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG14540), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143194.2

ORF Insert Sequence:

ATGAAGATGG TGCGCCCAAT TCGTCGCAAG GGAATCGAGG AGGTCTATGG TTCCAAAATG 
GATTCCTTGC ATCCGGTAAA GTCGGTAGCG ATATCCCATG CAGGTGTGAC CAAGAAAACA 
GCAAGGGCTG AGAGATCGGG TCCAGGCGGT GCAACTGGCG GTGGAGGAAC CATTAGCTCC 
CGACAAGTCG ATGCTCGCAC TCAGCGCAGT TTTGTCACCG AAAATGATTA CTACATCACC 
ACCAATCTGC CGTTGACGGC GGCCAATCTG CGAAATGCCC CCGGTTCGAT GGCGCAGCCA 
TCAATATCGC AAATGATTCC CGGCCATGTC CAGCCATCTC CATATAACGC CATGCCCTCG 
AGGCGAAACC AAATGGCCAA TAAGGCCAGT GCTCGTCAGG CGCCGATGAA CAAGGTGCCG 
CATTTTAGCC CGGCCGAAGG CACGGTAAAC CCTAATGGCT ATGGCATGTC CAAGTCGAAG 
GAGACATACC GAATGAAGCA CCGCGAAAAG GGCAATACCC AGAGGAACGA CTATCCAATG 
GATGACAACG ACGACTACTC CGATAAGGAA CTAATGGTGC CCCAGCAGGG AAACATGGGA 
AATATGCGCC GGCACAATCC GCACTCTCAT GCCCATCAAA TGCAAATGCA GTTGCAATCC 
GGAGCAATGC AGCATCCGCC GCAACACCAG CTGCACCAGC AGCAGCAGAT GACTACTCAC 
AACCACCTCA AGCATCAGCA ACAGCAGCGC CTCCAGCATT CGCAACCGCA TCAGCATCAG 
CATCAGCATC CGCATCCGCA TCAGAAGCAG ATGCGTGGGC GACAGCCTGT TCCGGCGAGC 
AAGGTGCACG TGAGTACCGC CGATGACGAT GAAGATAAGG AGGAAGATCC TGAGGAGTTC 
TTTGACCTGA TCCGCCAGAC TGTCAAAACG GCCGTTGGCA ACACCATATC CGATGCTCTG 
GCAAAGAACT TTCGCGATCT GAGCCACAAA ATCGAAAGGT TCTCGGGCGA GTTGAAGCAG 
ACAAACGATA ACCTGGATAA GCTGCAGGAA CAAGTCATTA GCAAGGTAAT TTACTTCGGT 
GAGGAAAATT CGCGACACTT TCGGTACTTG TGCATGAAAT CCGAGTACGA TAAAATGTTT 
TACCAGCATC AATCGCTGAT GAATGGAAAG CCCACTCAGG AAATGCTGAG TCTTTCGAAG 
GCGAACTTAG AAGCAGCGGC CTCAGTCAGT CAGAATCTGA CACAGAAACC GTCGCATAGT 
TTAAAACAAC AACCAGGAAA AGTGATTAGG AATCAAAGCA AAATGCCTAT TAGCGCCAAA 
AAAGACCATA ACGAGTCCCT AATGAACTCC AGTGCCTATC GTAGCATCTG CAAAGCTCAG 
CAGCCAGTTC TTGCTGCACA AAAGTCGTCA TCGGAGCAAA GTTTAGTTGT AAAATCATCT 
GATGTGGGCG TGCAAGAAGT CCTGGATAAT ATCCAGCGCT ACTGCAATCA GGTGAAGGAT 
ATGAATGGTC AGATGTCGGC TGAACTGCCC AGTCTCGAGG ACATCTTGAT ACCCAAGAAA 
AATTCAAACG GTGGACTTTG TCAGCCAGCA GCCATGAGCT CTAGGACCCT CGCAGGTAGT 
CAGAAAAATA AAAAGGTTGA CGACACGGAG ATCGAAACGC CCAACGATAG TACGGATGAG 
ACCTACACCG AAGTGGATGA CTTCCAGCTC TCCTCGGACA TCTCGACCGG CAGCGAGGAC 
GATGACTGTG GCCTCAAGTA TCCAAGTGGA GCAACTACGA GCCGGCCCAA CCGCAAATTA 
AATCGAAGGG GAGCCAACCA AGGCGCCGGA GATGGTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG14540 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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