twin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	twin
Entrez Gene ID	42880
Full Name	CG31137 gene product from transcript CG31137-RE
Synonyms	BEST:GH20274,CCR4,CG17741,CG31137,CG5534,CT39331,Ccr4,Dmel\CG31137,Twin,ccr4,twn
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95F1-95F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_170130.3	NP_732966.1	twin, isoform E
NM_170132.3	NP_732968.1	twin, isoform D
NM_170129.3	NP_732965.1	twin, isoform A
NM_176555.2	NP_788732.1	twin, isoform F
NM_170131.3	NP_732967.1	twin, isoform B
NM_170128.2	NP_732964.1	twin, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CNOT6L	XP_003310375.1
Mus musculus (house mouse)	Cnot6l	NP_849185.2
Rattus norvegicus (Norway rat)	Cnot6l	NP_001101825.1
Eremothecium gossypii	AGOS_ACR288W	NP_983690.1
Schizosaccharomyces pombe (fission yeast)	ccr4	NP_587901.1
Homo sapiens (human)	CNOT6L	NP_001273719.1
Caenorhabditis elegans (roundworm)	ccr-4	NP_502355.2
Kluyveromyces lactis	KLLA0F15884g	XP_455795.1
Neurospora crassa	NCU07779	XP_958700.2
Xenopus tropicalis (tropical clawed frog)	cnot6l	NP_001120421.1
Canis lupus familiaris (dog)	CNOT6L	XP_005639155.1
Saccharomyces cerevisiae (baker's yeast)	CCR4	NP_009381.1
Magnaporthe oryzae (rice blast fungus)	MGG_13020	XP_003710203.1
Danio rerio (zebrafish)	cnot6l	NP_001018474.1
Drosophila melanogaster (fruit fly)	twin	NP_732966.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004405	XP_313691.2
	CNOT6L	XP_001092697.2
Bos taurus (cattle)	CNOT6L	NP_001178452.1
Gallus gallus (chicken)	CNOT6L	XP_004941080.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CCR4 and CAF1, the two deadenylases in the CCR4-NOT complex, can remove 3' terminal non-A residues in an exonucleolytic manner.
Title: CCR4 and CAF1 deadenylases have an intrinsic activity to remove the post-poly(A) sequence.

Twin has important intrinsic roles in promoting GSC self-renewal and progeny differentiation by functioning in different protein complexes.
Title: Twin Promotes the Maintenance and Differentiation of Germline Stem Cell Lineage through Modulation of Multiple Pathways.

These results indicate that the Ccr4-Not complex controls expression of 4E-BP at multiple levels and adjusts the magnitude of the total effect.
Title: Multi-functional regulation of 4E-BP gene expression by the Ccr4-Not complex.

CCR4 deadenylase is required for germline stem cell self-renewal. Nos and Pum act through CCR4 recruitment onto specific mRNAs.
Title: The CCR4 deadenylase acts with Nanos and Pumilio in the fine-tuning of Mei-P26 expression to promote germline stem cell self-renewal.

Functions during early oogenesis to coordinate cyst division, oocyte fate specification and egg chamber maturation.
Title: twin, a CCR4 homolog, regulates cyclin poly(A) tail length to permit Drosophila oogenesis.

The following twin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05914	NM_170130.3 Latest version!	Drosophila melanogaster twin, transcript variant E (twin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05914	NM_170129.3 Latest version!	Drosophila melanogaster twin, transcript variant A (twin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05914	NM_176555.2 Latest version!	Drosophila melanogaster twin, transcript variant F (twin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05915	NM_170128.2 Latest version!	Drosophila melanogaster twin, transcript variant C (twin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05916	NM_170132.3 Latest version!	Drosophila melanogaster twin, transcript variant D (twin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05917	NM_170131.3 Latest version!	Drosophila melanogaster twin, transcript variant B (twin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twin ( NM_170130.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732966.1 Drosophila melanogaster twin, transcript variant E (twin), mRNA.

Price & Availability↑

CloneID	OFa05914
Clone ID Related Accession (Same CDS sequence)	NM_170130.3 , NM_170129.3 , NM_176555.2
Accession Version	NM_170130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170130.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGGCA ATCATTATAA AATGTCTCGT AACAAAGACA AATACGACAG CGCCAATCGG 
CGGCAGCAGA TCTTTCTGTC GCAGGAGGAC ATAGCTGCGG GCAAGAAGAC GAACTGGAGT 
GGCCTAGAGA TTACTGGCTG CGTCCGCAAT ATCAGCCCGT CGCTGTGGGA ATTTGAGCAT 
CTAACCGCCC TCTACCTGAA CGACAACCAG CTGCTGCGAT TGCCCGCAGA CGTTGGCATG 
CTGACCAGCC TCCGAACTTT GGACCTGTCC AGCAATAAGC TAAGAAGTCT TCCCGCAGAG 
CTCGGCGAGC TCATCCAGTT GCGGGAGCTG TTGCTGAACA ACAACTTTCT GCGCGTACTG 
CCTTACGAGA TCGGCAAGCT GTTCCACCTC GTCATACTCG GCCTCATGGG CAATCCGCTG 
CAAAAGGAGT TCATGAACAT CTACAACGAA CCGAACGGCA CGCAGAAACT GCTCACCTAC 
ATGCTGGACA ACTTGTCATT TACCGTGAAT CCACCGCCCC AGAGGCCCTG GCTGCCACTG 
GCCAAGCCCA ACAAAACGCG ACCAGCCTGC ATTTTTACGG TCATGTGCTA TAATGTGCTC 
TGCGACAAGT ACGCGACGCG ACAAATGTAC GGATACTGTC CGTCGTGGGC GCTATGCTGG 
GAGTACCGAA AAAAGTCGAT TATCGACGAG ATACGGCACT ATGCAGCGGA CATTATCAGT 
CTGCAGGAGA TCGAAACGGA GCAATTCTAT CACTTCTTCC TGCCGGAACT CAAGAACGAT 
GGGTACGAGG GAATCTTCTC ACCGAAGTCG CGTGCTAAGA CTATGTCCGA GCTGGAGAGG 
AAGTACGTCG ATGGCTGTGC GATATTCTTC AGGGCGTCCA AGTTTACGCT GATCAAGGAA 
TCATTGATCG AGTTCAATCA GCTGGCAATG GCCAATGCCG AGGGCTCCGA CAACATGCTG 
AACCGCGTAA TGCCTAAGGA TAACATCGGT CTGGCCGCAC TGCTCAAGGT GAAGGAGAAC 
GCATGGGAGC CGATGTCCGA GGTGACGCAG ATCTCGCAGC CGCTGCTCGT CTGCACGGCG 
CACATACACT GGGACCCTGA GTTCTGCGAC GTCAAGCTCA TCCAGACGAT GATGCTTAGC 
AATGAGTTGA AGACGATCAT CGACGAGGCG AGCCACAGTT TCCGACCTGG TCACAAGAAC 
GACTCCAATG CTGTCCAGCT GCTGCTGTGC GGTGACTTCA ACTCGCTACC CGATTCAGGC 
GTTGTGGAGT TTCTCGGCAA GGGCCGCGTT TCCATGGATC ATTTGGACTT CAAGGACATG 
GGCTACAAGT CCTGCCTGCA GCGGCTGCTC TCGAACGACA CCAACGAGTT TACGCACTCG 
TTCAAGCTAG CCTCCGCCTA CAACGAGGAC ATAATGCCGC ACACCAACTA TACGTTCGAT 
TTTAAGGGCA TCATCGACTA CATTTTCTAC ACGAAGACGG GCATGGTGCC GCTGGGCCTG 
CTGGGTCCTG TCTCCAATGA TTGGCTGCGC GAGAATAAGG TTGTTGGATG CCCACATCCG 
CATATACCCT CTGATCACTT CCCACTGCTG GTCGAGCTAG AGCTGATGCA TACGGCTAGC 
CAACAGGCGC CTCCTAACGG GCTGATCAAT CGCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732966.1
CDS	347..2005
Translation	MKGNHYKMSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLNDNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKLFHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSFTVNPPPQRPWLPLAKPNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR

Target ORF information:

RefSeq Version	NM_170130.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin, transcript variant E (twin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170130.3

ORF Insert Sequence:

ATGAAAGGCA ATCATTATAA AATGTCTCGT AACAAAGACA AATACGACAG CGCCAATCGG 
CGGCAGCAGA TCTTTCTGTC GCAGGAGGAC ATAGCTGCGG GCAAGAAGAC GAACTGGAGT 
GGCCTAGAGA TTACTGGCTG CGTCCGCAAT ATCAGCCCGT CGCTGTGGGA ATTTGAGCAT 
CTAACCGCCC TCTACCTGAA CGACAACCAG CTGCTGCGAT TGCCCGCAGA CGTTGGCATG 
CTGACCAGCC TCCGAACTTT GGACCTGTCC AGCAATAAGC TAAGAAGTCT TCCCGCAGAG 
CTCGGCGAGC TCATCCAGTT GCGGGAGCTG TTGCTGAACA ACAACTTTCT GCGCGTACTG 
CCTTACGAGA TCGGCAAGCT GTTCCACCTC GTCATACTCG GCCTCATGGG CAATCCGCTG 
CAAAAGGAGT TCATGAACAT CTACAACGAA CCGAACGGCA CGCAGAAACT GCTCACCTAC 
ATGCTGGACA ACTTGTCATT TACCGTGAAT CCACCGCCCC AGAGGCCCTG GCTGCCACTG 
GCCAAGCCCA ACAAAACGCG ACCAGCCTGC ATTTTTACGG TCATGTGCTA TAATGTGCTC 
TGCGACAAGT ACGCGACGCG ACAAATGTAC GGATACTGTC CGTCGTGGGC GCTATGCTGG 
GAGTACCGAA AAAAGTCGAT TATCGACGAG ATACGGCACT ATGCAGCGGA CATTATCAGT 
CTGCAGGAGA TCGAAACGGA GCAATTCTAT CACTTCTTCC TGCCGGAACT CAAGAACGAT 
GGGTACGAGG GAATCTTCTC ACCGAAGTCG CGTGCTAAGA CTATGTCCGA GCTGGAGAGG 
AAGTACGTCG ATGGCTGTGC GATATTCTTC AGGGCGTCCA AGTTTACGCT GATCAAGGAA 
TCATTGATCG AGTTCAATCA GCTGGCAATG GCCAATGCCG AGGGCTCCGA CAACATGCTG 
AACCGCGTAA TGCCTAAGGA TAACATCGGT CTGGCCGCAC TGCTCAAGGT GAAGGAGAAC 
GCATGGGAGC CGATGTCCGA GGTGACGCAG ATCTCGCAGC CGCTGCTCGT CTGCACGGCG 
CACATACACT GGGACCCTGA GTTCTGCGAC GTCAAGCTCA TCCAGACGAT GATGCTTAGC 
AATGAGTTGA AGACGATCAT CGACGAGGCG AGCCACAGTT TCCGACCTGG TCACAAGAAC 
GACTCCAATG CTGTCCAGCT GCTGCTGTGC GGTGACTTCA ACTCGCTACC CGATTCAGGC 
GTTGTGGAGT TTCTCGGCAA GGGCCGCGTT TCCATGGATC ATTTGGACTT CAAGGACATG 
GGCTACAAGT CCTGCCTGCA GCGGCTGCTC TCGAACGACA CCAACGAGTT TACGCACTCG 
TTCAAGCTAG CCTCCGCCTA CAACGAGGAC ATAATGCCGC ACACCAACTA TACGTTCGAT 
TTTAAGGGCA TCATCGACTA CATTTTCTAC ACGAAGACGG GCATGGTGCC GCTGGGCCTG 
CTGGGTCCTG TCTCCAATGA TTGGCTGCGC GAGAATAAGG TTGTTGGATG CCCACATCCG 
CATATACCCT CTGATCACTT CCCACTGCTG GTCGAGCTAG AGCTGATGCA TACGGCTAGC 
CAACAGGCGC CTCCTAACGG GCTGATCAAT CGCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twin ( NM_170129.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732965.1 Drosophila melanogaster twin, transcript variant A (twin), mRNA.

Price & Availability↑

CloneID	OFa05914
Clone ID Related Accession (Same CDS sequence)	NM_170130.3 , NM_170129.3 , NM_176555.2
Accession Version	NM_170129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170129.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGGCA ATCATTATAA AATGTCTCGT AACAAAGACA AATACGACAG CGCCAATCGG 
CGGCAGCAGA TCTTTCTGTC GCAGGAGGAC ATAGCTGCGG GCAAGAAGAC GAACTGGAGT 
GGCCTAGAGA TTACTGGCTG CGTCCGCAAT ATCAGCCCGT CGCTGTGGGA ATTTGAGCAT 
CTAACCGCCC TCTACCTGAA CGACAACCAG CTGCTGCGAT TGCCCGCAGA CGTTGGCATG 
CTGACCAGCC TCCGAACTTT GGACCTGTCC AGCAATAAGC TAAGAAGTCT TCCCGCAGAG 
CTCGGCGAGC TCATCCAGTT GCGGGAGCTG TTGCTGAACA ACAACTTTCT GCGCGTACTG 
CCTTACGAGA TCGGCAAGCT GTTCCACCTC GTCATACTCG GCCTCATGGG CAATCCGCTG 
CAAAAGGAGT TCATGAACAT CTACAACGAA CCGAACGGCA CGCAGAAACT GCTCACCTAC 
ATGCTGGACA ACTTGTCATT TACCGTGAAT CCACCGCCCC AGAGGCCCTG GCTGCCACTG 
GCCAAGCCCA ACAAAACGCG ACCAGCCTGC ATTTTTACGG TCATGTGCTA TAATGTGCTC 
TGCGACAAGT ACGCGACGCG ACAAATGTAC GGATACTGTC CGTCGTGGGC GCTATGCTGG 
GAGTACCGAA AAAAGTCGAT TATCGACGAG ATACGGCACT ATGCAGCGGA CATTATCAGT 
CTGCAGGAGA TCGAAACGGA GCAATTCTAT CACTTCTTCC TGCCGGAACT CAAGAACGAT 
GGGTACGAGG GAATCTTCTC ACCGAAGTCG CGTGCTAAGA CTATGTCCGA GCTGGAGAGG 
AAGTACGTCG ATGGCTGTGC GATATTCTTC AGGGCGTCCA AGTTTACGCT GATCAAGGAA 
TCATTGATCG AGTTCAATCA GCTGGCAATG GCCAATGCCG AGGGCTCCGA CAACATGCTG 
AACCGCGTAA TGCCTAAGGA TAACATCGGT CTGGCCGCAC TGCTCAAGGT GAAGGAGAAC 
GCATGGGAGC CGATGTCCGA GGTGACGCAG ATCTCGCAGC CGCTGCTCGT CTGCACGGCG 
CACATACACT GGGACCCTGA GTTCTGCGAC GTCAAGCTCA TCCAGACGAT GATGCTTAGC 
AATGAGTTGA AGACGATCAT CGACGAGGCG AGCCACAGTT TCCGACCTGG TCACAAGAAC 
GACTCCAATG CTGTCCAGCT GCTGCTGTGC GGTGACTTCA ACTCGCTACC CGATTCAGGC 
GTTGTGGAGT TTCTCGGCAA GGGCCGCGTT TCCATGGATC ATTTGGACTT CAAGGACATG 
GGCTACAAGT CCTGCCTGCA GCGGCTGCTC TCGAACGACA CCAACGAGTT TACGCACTCG 
TTCAAGCTAG CCTCCGCCTA CAACGAGGAC ATAATGCCGC ACACCAACTA TACGTTCGAT 
TTTAAGGGCA TCATCGACTA CATTTTCTAC ACGAAGACGG GCATGGTGCC GCTGGGCCTG 
CTGGGTCCTG TCTCCAATGA TTGGCTGCGC GAGAATAAGG TTGTTGGATG CCCACATCCG 
CATATACCCT CTGATCACTT CCCACTGCTG GTCGAGCTAG AGCTGATGCA TACGGCTAGC 
CAACAGGCGC CTCCTAACGG GCTGATCAAT CGCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732965.1
CDS	199..1857
Translation	MKGNHYKMSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLNDNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKLFHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSFTVNPPPQRPWLPLAKPNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR

Target ORF information:

RefSeq Version	NM_170129.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin, transcript variant A (twin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170129.3

ORF Insert Sequence:

ATGAAAGGCA ATCATTATAA AATGTCTCGT AACAAAGACA AATACGACAG CGCCAATCGG 
CGGCAGCAGA TCTTTCTGTC GCAGGAGGAC ATAGCTGCGG GCAAGAAGAC GAACTGGAGT 
GGCCTAGAGA TTACTGGCTG CGTCCGCAAT ATCAGCCCGT CGCTGTGGGA ATTTGAGCAT 
CTAACCGCCC TCTACCTGAA CGACAACCAG CTGCTGCGAT TGCCCGCAGA CGTTGGCATG 
CTGACCAGCC TCCGAACTTT GGACCTGTCC AGCAATAAGC TAAGAAGTCT TCCCGCAGAG 
CTCGGCGAGC TCATCCAGTT GCGGGAGCTG TTGCTGAACA ACAACTTTCT GCGCGTACTG 
CCTTACGAGA TCGGCAAGCT GTTCCACCTC GTCATACTCG GCCTCATGGG CAATCCGCTG 
CAAAAGGAGT TCATGAACAT CTACAACGAA CCGAACGGCA CGCAGAAACT GCTCACCTAC 
ATGCTGGACA ACTTGTCATT TACCGTGAAT CCACCGCCCC AGAGGCCCTG GCTGCCACTG 
GCCAAGCCCA ACAAAACGCG ACCAGCCTGC ATTTTTACGG TCATGTGCTA TAATGTGCTC 
TGCGACAAGT ACGCGACGCG ACAAATGTAC GGATACTGTC CGTCGTGGGC GCTATGCTGG 
GAGTACCGAA AAAAGTCGAT TATCGACGAG ATACGGCACT ATGCAGCGGA CATTATCAGT 
CTGCAGGAGA TCGAAACGGA GCAATTCTAT CACTTCTTCC TGCCGGAACT CAAGAACGAT 
GGGTACGAGG GAATCTTCTC ACCGAAGTCG CGTGCTAAGA CTATGTCCGA GCTGGAGAGG 
AAGTACGTCG ATGGCTGTGC GATATTCTTC AGGGCGTCCA AGTTTACGCT GATCAAGGAA 
TCATTGATCG AGTTCAATCA GCTGGCAATG GCCAATGCCG AGGGCTCCGA CAACATGCTG 
AACCGCGTAA TGCCTAAGGA TAACATCGGT CTGGCCGCAC TGCTCAAGGT GAAGGAGAAC 
GCATGGGAGC CGATGTCCGA GGTGACGCAG ATCTCGCAGC CGCTGCTCGT CTGCACGGCG 
CACATACACT GGGACCCTGA GTTCTGCGAC GTCAAGCTCA TCCAGACGAT GATGCTTAGC 
AATGAGTTGA AGACGATCAT CGACGAGGCG AGCCACAGTT TCCGACCTGG TCACAAGAAC 
GACTCCAATG CTGTCCAGCT GCTGCTGTGC GGTGACTTCA ACTCGCTACC CGATTCAGGC 
GTTGTGGAGT TTCTCGGCAA GGGCCGCGTT TCCATGGATC ATTTGGACTT CAAGGACATG 
GGCTACAAGT CCTGCCTGCA GCGGCTGCTC TCGAACGACA CCAACGAGTT TACGCACTCG 
TTCAAGCTAG CCTCCGCCTA CAACGAGGAC ATAATGCCGC ACACCAACTA TACGTTCGAT 
TTTAAGGGCA TCATCGACTA CATTTTCTAC ACGAAGACGG GCATGGTGCC GCTGGGCCTG 
CTGGGTCCTG TCTCCAATGA TTGGCTGCGC GAGAATAAGG TTGTTGGATG CCCACATCCG 
CATATACCCT CTGATCACTT CCCACTGCTG GTCGAGCTAG AGCTGATGCA TACGGCTAGC 
CAACAGGCGC CTCCTAACGG GCTGATCAAT CGCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twin ( NM_176555.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788732.1 Drosophila melanogaster twin, transcript variant F (twin), mRNA.

Price & Availability↑

CloneID	OFa05914
Clone ID Related Accession (Same CDS sequence)	NM_170130.3 , NM_170129.3 , NM_176555.2
Accession Version	NM_176555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176555.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGGCA ATCATTATAA AATGTCTCGT AACAAAGACA AATACGACAG CGCCAATCGG 
CGGCAGCAGA TCTTTCTGTC GCAGGAGGAC ATAGCTGCGG GCAAGAAGAC GAACTGGAGT 
GGCCTAGAGA TTACTGGCTG CGTCCGCAAT ATCAGCCCGT CGCTGTGGGA ATTTGAGCAT 
CTAACCGCCC TCTACCTGAA CGACAACCAG CTGCTGCGAT TGCCCGCAGA CGTTGGCATG 
CTGACCAGCC TCCGAACTTT GGACCTGTCC AGCAATAAGC TAAGAAGTCT TCCCGCAGAG 
CTCGGCGAGC TCATCCAGTT GCGGGAGCTG TTGCTGAACA ACAACTTTCT GCGCGTACTG 
CCTTACGAGA TCGGCAAGCT GTTCCACCTC GTCATACTCG GCCTCATGGG CAATCCGCTG 
CAAAAGGAGT TCATGAACAT CTACAACGAA CCGAACGGCA CGCAGAAACT GCTCACCTAC 
ATGCTGGACA ACTTGTCATT TACCGTGAAT CCACCGCCCC AGAGGCCCTG GCTGCCACTG 
GCCAAGCCCA ACAAAACGCG ACCAGCCTGC ATTTTTACGG TCATGTGCTA TAATGTGCTC 
TGCGACAAGT ACGCGACGCG ACAAATGTAC GGATACTGTC CGTCGTGGGC GCTATGCTGG 
GAGTACCGAA AAAAGTCGAT TATCGACGAG ATACGGCACT ATGCAGCGGA CATTATCAGT 
CTGCAGGAGA TCGAAACGGA GCAATTCTAT CACTTCTTCC TGCCGGAACT CAAGAACGAT 
GGGTACGAGG GAATCTTCTC ACCGAAGTCG CGTGCTAAGA CTATGTCCGA GCTGGAGAGG 
AAGTACGTCG ATGGCTGTGC GATATTCTTC AGGGCGTCCA AGTTTACGCT GATCAAGGAA 
TCATTGATCG AGTTCAATCA GCTGGCAATG GCCAATGCCG AGGGCTCCGA CAACATGCTG 
AACCGCGTAA TGCCTAAGGA TAACATCGGT CTGGCCGCAC TGCTCAAGGT GAAGGAGAAC 
GCATGGGAGC CGATGTCCGA GGTGACGCAG ATCTCGCAGC CGCTGCTCGT CTGCACGGCG 
CACATACACT GGGACCCTGA GTTCTGCGAC GTCAAGCTCA TCCAGACGAT GATGCTTAGC 
AATGAGTTGA AGACGATCAT CGACGAGGCG AGCCACAGTT TCCGACCTGG TCACAAGAAC 
GACTCCAATG CTGTCCAGCT GCTGCTGTGC GGTGACTTCA ACTCGCTACC CGATTCAGGC 
GTTGTGGAGT TTCTCGGCAA GGGCCGCGTT TCCATGGATC ATTTGGACTT CAAGGACATG 
GGCTACAAGT CCTGCCTGCA GCGGCTGCTC TCGAACGACA CCAACGAGTT TACGCACTCG 
TTCAAGCTAG CCTCCGCCTA CAACGAGGAC ATAATGCCGC ACACCAACTA TACGTTCGAT 
TTTAAGGGCA TCATCGACTA CATTTTCTAC ACGAAGACGG GCATGGTGCC GCTGGGCCTG 
CTGGGTCCTG TCTCCAATGA TTGGCTGCGC GAGAATAAGG TTGTTGGATG CCCACATCCG 
CATATACCCT CTGATCACTT CCCACTGCTG GTCGAGCTAG AGCTGATGCA TACGGCTAGC 
CAACAGGCGC CTCCTAACGG GCTGATCAAT CGCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788732.1
CDS	187..1845
Translation	MKGNHYKMSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLNDNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKLFHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSFTVNPPPQRPWLPLAKPNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR

Target ORF information:

RefSeq Version	NM_176555.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin, transcript variant F (twin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176555.2

ORF Insert Sequence:

ATGAAAGGCA ATCATTATAA AATGTCTCGT AACAAAGACA AATACGACAG CGCCAATCGG 
CGGCAGCAGA TCTTTCTGTC GCAGGAGGAC ATAGCTGCGG GCAAGAAGAC GAACTGGAGT 
GGCCTAGAGA TTACTGGCTG CGTCCGCAAT ATCAGCCCGT CGCTGTGGGA ATTTGAGCAT 
CTAACCGCCC TCTACCTGAA CGACAACCAG CTGCTGCGAT TGCCCGCAGA CGTTGGCATG 
CTGACCAGCC TCCGAACTTT GGACCTGTCC AGCAATAAGC TAAGAAGTCT TCCCGCAGAG 
CTCGGCGAGC TCATCCAGTT GCGGGAGCTG TTGCTGAACA ACAACTTTCT GCGCGTACTG 
CCTTACGAGA TCGGCAAGCT GTTCCACCTC GTCATACTCG GCCTCATGGG CAATCCGCTG 
CAAAAGGAGT TCATGAACAT CTACAACGAA CCGAACGGCA CGCAGAAACT GCTCACCTAC 
ATGCTGGACA ACTTGTCATT TACCGTGAAT CCACCGCCCC AGAGGCCCTG GCTGCCACTG 
GCCAAGCCCA ACAAAACGCG ACCAGCCTGC ATTTTTACGG TCATGTGCTA TAATGTGCTC 
TGCGACAAGT ACGCGACGCG ACAAATGTAC GGATACTGTC CGTCGTGGGC GCTATGCTGG 
GAGTACCGAA AAAAGTCGAT TATCGACGAG ATACGGCACT ATGCAGCGGA CATTATCAGT 
CTGCAGGAGA TCGAAACGGA GCAATTCTAT CACTTCTTCC TGCCGGAACT CAAGAACGAT 
GGGTACGAGG GAATCTTCTC ACCGAAGTCG CGTGCTAAGA CTATGTCCGA GCTGGAGAGG 
AAGTACGTCG ATGGCTGTGC GATATTCTTC AGGGCGTCCA AGTTTACGCT GATCAAGGAA 
TCATTGATCG AGTTCAATCA GCTGGCAATG GCCAATGCCG AGGGCTCCGA CAACATGCTG 
AACCGCGTAA TGCCTAAGGA TAACATCGGT CTGGCCGCAC TGCTCAAGGT GAAGGAGAAC 
GCATGGGAGC CGATGTCCGA GGTGACGCAG ATCTCGCAGC CGCTGCTCGT CTGCACGGCG 
CACATACACT GGGACCCTGA GTTCTGCGAC GTCAAGCTCA TCCAGACGAT GATGCTTAGC 
AATGAGTTGA AGACGATCAT CGACGAGGCG AGCCACAGTT TCCGACCTGG TCACAAGAAC 
GACTCCAATG CTGTCCAGCT GCTGCTGTGC GGTGACTTCA ACTCGCTACC CGATTCAGGC 
GTTGTGGAGT TTCTCGGCAA GGGCCGCGTT TCCATGGATC ATTTGGACTT CAAGGACATG 
GGCTACAAGT CCTGCCTGCA GCGGCTGCTC TCGAACGACA CCAACGAGTT TACGCACTCG 
TTCAAGCTAG CCTCCGCCTA CAACGAGGAC ATAATGCCGC ACACCAACTA TACGTTCGAT 
TTTAAGGGCA TCATCGACTA CATTTTCTAC ACGAAGACGG GCATGGTGCC GCTGGGCCTG 
CTGGGTCCTG TCTCCAATGA TTGGCTGCGC GAGAATAAGG TTGTTGGATG CCCACATCCG 
CATATACCCT CTGATCACTT CCCACTGCTG GTCGAGCTAG AGCTGATGCA TACGGCTAGC 
CAACAGGCGC CTCCTAACGG GCTGATCAAT CGCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twin ( NM_170128.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732964.1 Drosophila melanogaster twin, transcript variant C (twin), mRNA.

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CloneID	OFa05915
Clone ID Related Accession (Same CDS sequence)	NM_170128.2
Accession Version	NM_170128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170128.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTATCT TTAAAAAAAG AGACGCTTAT CATCAGGCGG TTATTATGAA AGGCAATCAT 
TATAAAATGT CTCGTAACAA AGACAAATAC GACAGCGCCA ATCGGCGGCA GCAGATCTTT 
CTGTCGCAGG AGGACATAGC TGCGGGCAAG AAGACGAACT GGAGTGGCCT AGAGATTACT 
GGCTGCGTCC GCAATATCAG CCCGTCGCTG TGGGAATTTG AGCATCTAAC CGCCCTCTAC 
CTGAACGACA ACCAGCTGCT GCGATTGCCC GCAGACGTTG GCATGCTGAC CAGCCTCCGA 
ACTTTGGACC TGTCCAGCAA TAAGCTAAGA AGTCTTCCCG CAGAGCTCGG CGAGCTCATC 
CAGTTGCGGG AGCTGTTGCT GAACAACAAC TTTCTGCGCG TACTGCCTTA CGAGATCGGC 
AAGCTGTTCC ACCTCGTCAT ACTCGGCCTC ATGGGCAATC CGCTGCAAAA GGAGTTCATG 
AACATCTACA ACGAACCGAA CGGCACGCAG AAACTGCTCA CCTACATGCT GGACAACTTG 
TCATTTACCG TGAATCCACC GCCCCAGAGG CCCTGGCTGC CACTGGCCAA GCCCAACAAA 
ACGCGACCAG CCTGCATTTT TACGGTCATG TGCTATAATG TGCTCTGCGA CAAGTACGCG 
ACGCGACAAA TGTACGGATA CTGTCCGTCG TGGGCGCTAT GCTGGGAGTA CCGAAAAAAG 
TCGATTATCG ACGAGATACG GCACTATGCA GCGGACATTA TCAGTCTGCA GGAGATCGAA 
ACGGAGCAAT TCTATCACTT CTTCCTGCCG GAACTCAAGA ACGATGGGTA CGAGGGAATC 
TTCTCACCGA AGTCGCGTGC TAAGACTATG TCCGAGCTGG AGAGGAAGTA CGTCGATGGC 
TGTGCGATAT TCTTCAGGGC GTCCAAGTTT ACGCTGATCA AGGAATCATT GATCGAGTTC 
AATCAGCTGG CAATGGCCAA TGCCGAGGGC TCCGACAACA TGCTGAACCG CGTAATGCCT 
AAGGATAACA TCGGTCTGGC CGCACTGCTC AAGGTGAAGG AGAACGCATG GGAGCCGATG 
TCCGAGGTGA CGCAGATCTC GCAGCCGCTG CTCGTCTGCA CGGCGCACAT ACACTGGGAC 
CCTGAGTTCT GCGACGTCAA GCTCATCCAG ACGATGATGC TTAGCAATGA GTTGAAGACG 
ATCATCGACG AGGCGAGCCA CAGTTTCCGA CCTGGTCACA AGAACGACTC CAATGCTGTC 
CAGCTGCTGC TGTGCGGTGA CTTCAACTCG CTACCCGATT CAGGCGTTGT GGAGTTTCTC 
GGCAAGGGCC GCGTTTCCAT GGATCATTTG GACTTCAAGG ACATGGGCTA CAAGTCCTGC 
CTGCAGCGGC TGCTCTCGAA CGACACCAAC GAGTTTACGC ACTCGTTCAA GCTAGCCTCC 
GCCTACAACG AGGACATAAT GCCGCACACC AACTATACGT TCGATTTTAA GGGCATCATC 
GACTACATTT TCTACACGAA GACGGGCATG GTGCCGCTGG GCCTGCTGGG TCCTGTCTCC 
AATGATTGGC TGCGCGAGAA TAAGGTTGTT GGATGCCCAC ATCCGCATAT ACCCTCTGAT 
CACTTCCCAC TGCTGGTCGA GCTAGAGCTG ATGCATACGG CTAGCCAACA GGCGCCTCCT 
AACGGGCTGA TCAATCGCCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732964.1
CDS	38..1741
Translation	MCIFKKRDAYHQAVIMKGNHYKMSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLNDNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKLFHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSFTVNPPPQRPWLPLAKPNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR

Target ORF information:

RefSeq Version	NM_170128.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin, transcript variant C (twin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170128.2

ORF Insert Sequence:

ATGTGTATCT TTAAAAAAAG AGACGCTTAT CATCAGGCGG TTATTATGAA AGGCAATCAT 
TATAAAATGT CTCGTAACAA AGACAAATAC GACAGCGCCA ATCGGCGGCA GCAGATCTTT 
CTGTCGCAGG AGGACATAGC TGCGGGCAAG AAGACGAACT GGAGTGGCCT AGAGATTACT 
GGCTGCGTCC GCAATATCAG CCCGTCGCTG TGGGAATTTG AGCATCTAAC CGCCCTCTAC 
CTGAACGACA ACCAGCTGCT GCGATTGCCC GCAGACGTTG GCATGCTGAC CAGCCTCCGA 
ACTTTGGACC TGTCCAGCAA TAAGCTAAGA AGTCTTCCCG CAGAGCTCGG CGAGCTCATC 
CAGTTGCGGG AGCTGTTGCT GAACAACAAC TTTCTGCGCG TACTGCCTTA CGAGATCGGC 
AAGCTGTTCC ACCTCGTCAT ACTCGGCCTC ATGGGCAATC CGCTGCAAAA GGAGTTCATG 
AACATCTACA ACGAACCGAA CGGCACGCAG AAACTGCTCA CCTACATGCT GGACAACTTG 
TCATTTACCG TGAATCCACC GCCCCAGAGG CCCTGGCTGC CACTGGCCAA GCCCAACAAA 
ACGCGACCAG CCTGCATTTT TACGGTCATG TGCTATAATG TGCTCTGCGA CAAGTACGCG 
ACGCGACAAA TGTACGGATA CTGTCCGTCG TGGGCGCTAT GCTGGGAGTA CCGAAAAAAG 
TCGATTATCG ACGAGATACG GCACTATGCA GCGGACATTA TCAGTCTGCA GGAGATCGAA 
ACGGAGCAAT TCTATCACTT CTTCCTGCCG GAACTCAAGA ACGATGGGTA CGAGGGAATC 
TTCTCACCGA AGTCGCGTGC TAAGACTATG TCCGAGCTGG AGAGGAAGTA CGTCGATGGC 
TGTGCGATAT TCTTCAGGGC GTCCAAGTTT ACGCTGATCA AGGAATCATT GATCGAGTTC 
AATCAGCTGG CAATGGCCAA TGCCGAGGGC TCCGACAACA TGCTGAACCG CGTAATGCCT 
AAGGATAACA TCGGTCTGGC CGCACTGCTC AAGGTGAAGG AGAACGCATG GGAGCCGATG 
TCCGAGGTGA CGCAGATCTC GCAGCCGCTG CTCGTCTGCA CGGCGCACAT ACACTGGGAC 
CCTGAGTTCT GCGACGTCAA GCTCATCCAG ACGATGATGC TTAGCAATGA GTTGAAGACG 
ATCATCGACG AGGCGAGCCA CAGTTTCCGA CCTGGTCACA AGAACGACTC CAATGCTGTC 
CAGCTGCTGC TGTGCGGTGA CTTCAACTCG CTACCCGATT CAGGCGTTGT GGAGTTTCTC 
GGCAAGGGCC GCGTTTCCAT GGATCATTTG GACTTCAAGG ACATGGGCTA CAAGTCCTGC 
CTGCAGCGGC TGCTCTCGAA CGACACCAAC GAGTTTACGC ACTCGTTCAA GCTAGCCTCC 
GCCTACAACG AGGACATAAT GCCGCACACC AACTATACGT TCGATTTTAA GGGCATCATC 
GACTACATTT TCTACACGAA GACGGGCATG GTGCCGCTGG GCCTGCTGGG TCCTGTCTCC 
AATGATTGGC TGCGCGAGAA TAAGGTTGTT GGATGCCCAC ATCCGCATAT ACCCTCTGAT 
CACTTCCCAC TGCTGGTCGA GCTAGAGCTG ATGCATACGG CTAGCCAACA GGCGCCTCCT 
AACGGGCTGA TCAATCGCCG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twin ( NM_170132.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732968.1 Drosophila melanogaster twin, transcript variant D (twin), mRNA.

Price & Availability↑

CloneID	OFa05916
Clone ID Related Accession (Same CDS sequence)	NM_170132.3
Accession Version	NM_170132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_170132.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCTATA ATGTGCTCTG CGACAAGTAC GCGACGCGAC AAATGTACGG ATACTGTCCG 
TCGTGGGCGC TATGCTGGGA GTACCGAAAA AAGTCGATTA TCGACGAGAT ACGGCACTAT 
GCAGCGGACA TTATCAGTCT GCAGGAGATC GAAACGGAGC AATTCTATCA CTTCTTCCTG 
CCGGAACTCA AGAACGATGG GTACGAGGGA ATCTTCTCAC CGAAGTCGCG TGCTAAGACT 
ATGTCCGAGC TGGAGAGGAA GTACGTCGAT GGCTGTGCGA TATTCTTCAG GGCGTCCAAG 
TTTACGCTGA TCAAGGAATC ATTGATCGAG TTCAATCAGC TGGCAATGGC CAATGCCGAG 
GGCTCCGACA ACATGCTGAA CCGCGTAATG CCTAAGGATA ACATCGGTCT GGCCGCACTG 
CTCAAGGTGA AGGAGAACGC ATGGGAGCCG ATGTCCGAGG TGACGCAGAT CTCGCAGCCG 
CTGCTCGTCT GCACGGCGCA CATACACTGG GACCCTGAGT TCTGCGACGT CAAGCTCATC 
CAGACGATGA TGCTTAGCAA TGAGTTGAAG ACGATCATCG ACGAGGCGAG CCACAGTTTC 
CGACCTGGTC ACAAGAACGA CTCCAATGCT GTCCAGCTGC TGCTGTGCGG TGACTTCAAC 
TCGCTACCCG ATTCAGGCGT TGTGGAGTTT CTCGGCAAGG GCCGCGTTTC CATGGATCAT 
TTGGACTTCA AGGACATGGG CTACAAGTCC TGCCTGCAGC GGCTGCTCTC GAACGACACC 
AACGAGTTTA CGCACTCGTT CAAGCTAGCC TCCGCCTACA ACGAGGACAT AATGCCGCAC 
ACCAACTATA CGTTCGATTT TAAGGGCATC ATCGACTACA TTTTCTACAC GAAGACGGGC 
ATGGTGCCGC TGGGCCTGCT GGGTCCTGTC TCCAATGATT GGCTGCGCGA GAATAAGGTT 
GTTGGATGCC CACATCCGCA TATACCCTCT GATCACTTCC CACTGCTGGT CGAGCTAGAG 
CTGATGCATA CGGCTAGCCA ACAGGCGCCT CCTAACGGGC TGATCAATCG CCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732968.1
CDS	1029..2105
Translation	MCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR

Target ORF information:

RefSeq Version	NM_170132.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin, transcript variant D (twin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170132.3

ORF Insert Sequence:

ATGTGCTATA ATGTGCTCTG CGACAAGTAC GCGACGCGAC AAATGTACGG ATACTGTCCG 
TCGTGGGCGC TATGCTGGGA GTACCGAAAA AAGTCGATTA TCGACGAGAT ACGGCACTAT 
GCAGCGGACA TTATCAGTCT GCAGGAGATC GAAACGGAGC AATTCTATCA CTTCTTCCTG 
CCGGAACTCA AGAACGATGG GTACGAGGGA ATCTTCTCAC CGAAGTCGCG TGCTAAGACT 
ATGTCCGAGC TGGAGAGGAA GTACGTCGAT GGCTGTGCGA TATTCTTCAG GGCGTCCAAG 
TTTACGCTGA TCAAGGAATC ATTGATCGAG TTCAATCAGC TGGCAATGGC CAATGCCGAG 
GGCTCCGACA ACATGCTGAA CCGCGTAATG CCTAAGGATA ACATCGGTCT GGCCGCACTG 
CTCAAGGTGA AGGAGAACGC ATGGGAGCCG ATGTCCGAGG TGACGCAGAT CTCGCAGCCG 
CTGCTCGTCT GCACGGCGCA CATACACTGG GACCCTGAGT TCTGCGACGT CAAGCTCATC 
CAGACGATGA TGCTTAGCAA TGAGTTGAAG ACGATCATCG ACGAGGCGAG CCACAGTTTC 
CGACCTGGTC ACAAGAACGA CTCCAATGCT GTCCAGCTGC TGCTGTGCGG TGACTTCAAC 
TCGCTACCCG ATTCAGGCGT TGTGGAGTTT CTCGGCAAGG GCCGCGTTTC CATGGATCAT 
TTGGACTTCA AGGACATGGG CTACAAGTCC TGCCTGCAGC GGCTGCTCTC GAACGACACC 
AACGAGTTTA CGCACTCGTT CAAGCTAGCC TCCGCCTACA ACGAGGACAT AATGCCGCAC 
ACCAACTATA CGTTCGATTT TAAGGGCATC ATCGACTACA TTTTCTACAC GAAGACGGGC 
ATGGTGCCGC TGGGCCTGCT GGGTCCTGTC TCCAATGATT GGCTGCGCGA GAATAAGGTT 
GTTGGATGCC CACATCCGCA TATACCCTCT GATCACTTCC CACTGCTGGT CGAGCTAGAG 
CTGATGCATA CGGCTAGCCA ACAGGCGCCT CCTAACGGGC TGATCAATCG CCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

twin ( NM_170131.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732967.1 Drosophila melanogaster twin, transcript variant B (twin), mRNA.

Price & Availability↑

CloneID	OFa05917
Clone ID Related Accession (Same CDS sequence)	NM_170131.3
Accession Version	NM_170131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170131.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGTA ACAAAGACAA ATACGACAGC GCCAATCGGC GGCAGCAGAT CTTTCTGTCG 
CAGGAGGACA TAGCTGCGGG CAAGAAGACG AACTGGAGTG GCCTAGAGAT TACTGGCTGC 
GTCCGCAATA TCAGCCCGTC GCTGTGGGAA TTTGAGCATC TAACCGCCCT CTACCTGAAC 
GACAACCAGC TGCTGCGATT GCCCGCAGAC GTTGGCATGC TGACCAGCCT CCGAACTTTG 
GACCTGTCCA GCAATAAGCT AAGAAGTCTT CCCGCAGAGC TCGGCGAGCT CATCCAGTTG 
CGGGAGCTGT TGCTGAACAA CAACTTTCTG CGCGTACTGC CTTACGAGAT CGGCAAGCTG 
TTCCACCTCG TCATACTCGG CCTCATGGGC AATCCGCTGC AAAAGGAGTT CATGAACATC 
TACAACGAAC CGAACGGCAC GCAGAAACTG CTCACCTACA TGCTGGACAA CTTGTCATTT 
ACCGTGAATC CACCGCCCCA GAGGCCCTGG CTGCCACTGG CCAAGCCCAA CAAAACGCGA 
CCAGCCTGCA TTTTTACGGT CATGTGCTAT AATGTGCTCT GCGACAAGTA CGCGACGCGA 
CAAATGTACG GATACTGTCC GTCGTGGGCG CTATGCTGGG AGTACCGAAA AAAGTCGATT 
ATCGACGAGA TACGGCACTA TGCAGCGGAC ATTATCAGTC TGCAGGAGAT CGAAACGGAG 
CAATTCTATC ACTTCTTCCT GCCGGAACTC AAGAACGATG GGTACGAGGG AATCTTCTCA 
CCGAAGTCGC GTGCTAAGAC TATGTCCGAG CTGGAGAGGA AGTACGTCGA TGGCTGTGCG 
ATATTCTTCA GGGCGTCCAA GTTTACGCTG ATCAAGGAAT CATTGATCGA GTTCAATCAG 
CTGGCAATGG CCAATGCCGA GGGCTCCGAC AACATGCTGA ACCGCGTAAT GCCTAAGGAT 
AACATCGGTC TGGCCGCACT GCTCAAGGTG AAGGAGAACG CATGGGAGCC GATGTCCGAG 
GTGACGCAGA TCTCGCAGCC GCTGCTCGTC TGCACGGCGC ACATACACTG GGACCCTGAG 
TTCTGCGACG TCAAGCTCAT CCAGACGATG ATGCTTAGCA ATGAGTTGAA GACGATCATC 
GACGAGGCGA GCCACAGTTT CCGACCTGGT CACAAGAACG ACTCCAATGC TGTCCAGCTG 
CTGCTGTGCG GTGACTTCAA CTCGCTACCC GATTCAGGCG TTGTGGAGTT TCTCGGCAAG 
GGCCGCGTTT CCATGGATCA TTTGGACTTC AAGGACATGG GCTACAAGTC CTGCCTGCAG 
CGGCTGCTCT CGAACGACAC CAACGAGTTT ACGCACTCGT TCAAGCTAGC CTCCGCCTAC 
AACGAGGACA TAATGCCGCA CACCAACTAT ACGTTCGATT TTAAGGGCAT CATCGACTAC 
ATTTTCTACA CGAAGACGGG CATGGTGCCG CTGGGCCTGC TGGGTCCTGT CTCCAATGAT 
TGGCTGCGCG AGAATAAGGT TGTTGGATGC CCACATCCGC ATATACCCTC TGATCACTTC 
CCACTGCTGG TCGAGCTAGA GCTGATGCAT ACGGCTAGCC AACAGGCGCC TCCTAACGGG 
CTGATCAATC GCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732967.1
CDS	336..1973
Translation	MSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLNDNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKLFHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSFTVNPPPQRPWLPLAKPNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR

Target ORF information:

RefSeq Version	NM_170131.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twin, transcript variant B (twin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170131.3

ORF Insert Sequence:

ATGTCTCGTA ACAAAGACAA ATACGACAGC GCCAATCGGC GGCAGCAGAT CTTTCTGTCG 
CAGGAGGACA TAGCTGCGGG CAAGAAGACG AACTGGAGTG GCCTAGAGAT TACTGGCTGC 
GTCCGCAATA TCAGCCCGTC GCTGTGGGAA TTTGAGCATC TAACCGCCCT CTACCTGAAC 
GACAACCAGC TGCTGCGATT GCCCGCAGAC GTTGGCATGC TGACCAGCCT CCGAACTTTG 
GACCTGTCCA GCAATAAGCT AAGAAGTCTT CCCGCAGAGC TCGGCGAGCT CATCCAGTTG 
CGGGAGCTGT TGCTGAACAA CAACTTTCTG CGCGTACTGC CTTACGAGAT CGGCAAGCTG 
TTCCACCTCG TCATACTCGG CCTCATGGGC AATCCGCTGC AAAAGGAGTT CATGAACATC 
TACAACGAAC CGAACGGCAC GCAGAAACTG CTCACCTACA TGCTGGACAA CTTGTCATTT 
ACCGTGAATC CACCGCCCCA GAGGCCCTGG CTGCCACTGG CCAAGCCCAA CAAAACGCGA 
CCAGCCTGCA TTTTTACGGT CATGTGCTAT AATGTGCTCT GCGACAAGTA CGCGACGCGA 
CAAATGTACG GATACTGTCC GTCGTGGGCG CTATGCTGGG AGTACCGAAA AAAGTCGATT 
ATCGACGAGA TACGGCACTA TGCAGCGGAC ATTATCAGTC TGCAGGAGAT CGAAACGGAG 
CAATTCTATC ACTTCTTCCT GCCGGAACTC AAGAACGATG GGTACGAGGG AATCTTCTCA 
CCGAAGTCGC GTGCTAAGAC TATGTCCGAG CTGGAGAGGA AGTACGTCGA TGGCTGTGCG 
ATATTCTTCA GGGCGTCCAA GTTTACGCTG ATCAAGGAAT CATTGATCGA GTTCAATCAG 
CTGGCAATGG CCAATGCCGA GGGCTCCGAC AACATGCTGA ACCGCGTAAT GCCTAAGGAT 
AACATCGGTC TGGCCGCACT GCTCAAGGTG AAGGAGAACG CATGGGAGCC GATGTCCGAG 
GTGACGCAGA TCTCGCAGCC GCTGCTCGTC TGCACGGCGC ACATACACTG GGACCCTGAG 
TTCTGCGACG TCAAGCTCAT CCAGACGATG ATGCTTAGCA ATGAGTTGAA GACGATCATC 
GACGAGGCGA GCCACAGTTT CCGACCTGGT CACAAGAACG ACTCCAATGC TGTCCAGCTG 
CTGCTGTGCG GTGACTTCAA CTCGCTACCC GATTCAGGCG TTGTGGAGTT TCTCGGCAAG 
GGCCGCGTTT CCATGGATCA TTTGGACTTC AAGGACATGG GCTACAAGTC CTGCCTGCAG 
CGGCTGCTCT CGAACGACAC CAACGAGTTT ACGCACTCGT TCAAGCTAGC CTCCGCCTAC 
AACGAGGACA TAATGCCGCA CACCAACTAT ACGTTCGATT TTAAGGGCAT CATCGACTAC 
ATTTTCTACA CGAAGACGGG CATGGTGCCG CTGGGCCTGC TGGGTCCTGT CTCCAATGAT 
TGGCTGCGCG AGAATAAGGT TGTTGGATGC CCACATCCGC ATATACCCTC TGATCACTTC 
CCACTGCTGG TCGAGCTAGA GCTGATGCAT ACGGCTAGCC AACAGGCGCC TCCTAACGGG 
CTGATCAATC GCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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twin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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