CCR4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CCR4
Entrez Gene ID	851212
Full Name	CCR4-NOT core exoribonuclease subunit CCR4
Synonyms	FUN27,NUT21
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178166.1	NP_009381.1	CCR4-NOT core exoribonuclease subunit CCR4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CNOT6L	XP_003310375.1
Mus musculus (house mouse)	Cnot6l	NP_849185.2
Rattus norvegicus (Norway rat)	Cnot6l	NP_001101825.1
Eremothecium gossypii	AGOS_ACR288W	NP_983690.1
Schizosaccharomyces pombe (fission yeast)	ccr4	NP_587901.1
Homo sapiens (human)	CNOT6L	NP_001273719.1
Caenorhabditis elegans (roundworm)	ccr-4	NP_502355.2
Kluyveromyces lactis	KLLA0F15884g	XP_455795.1
Neurospora crassa	NCU07779	XP_958700.2
Xenopus tropicalis (tropical clawed frog)	cnot6l	NP_001120421.1
Canis lupus familiaris (dog)	CNOT6L	XP_005639155.1
Saccharomyces cerevisiae (baker's yeast)	CCR4	NP_009381.1
Magnaporthe oryzae (rice blast fungus)	MGG_13020	XP_003710203.1
Danio rerio (zebrafish)	cnot6l	NP_001018474.1
Drosophila melanogaster (fruit fly)	twin	NP_732966.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004405	XP_313691.2
	CNOT6L	XP_001092697.2
Bos taurus (cattle)	CNOT6L	NP_001178452.1
Gallus gallus (chicken)	CNOT6L	XP_004941080.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey H, Kaback DB, Zhong W, Vo DT, Clark MW, Fortin N, Hall J, Ouellette BF, Keng T, Barton AB
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Ccr4 regulates not only LRG1 mRNA level through poly(A) shortening but also the translation of LRG1 mRNA, and that Pbp1 is involved in the Ccr4-mediated regulation of mRNA stability and translation.
Title: Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase.

Studies indicate that the Ccr4 (carbon catabolite repressed 4)-Not (negative on TATA-less) complex has been connected to every step of the mRNA lifecycle.
Title: Ccr4-Not is at the core of the eukaryotic gene expression circuitry.

Collectively, these data demonstrate a novel role for Ccr4-Not in Pol I transcriptional regulation that is required for bridging mTORC1 signaling to ribosomal RNA synthesis.
Title: Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis.

This review will describe what is known about the Ccr4-Not complex in regulating transcription elongation and its possible collaboration with other factors traveling with RNAPII across genes.
Title: The control of elongation by the yeast Ccr4-not complex.

A MIF4G domain positioned centrally within the Not1 protein recognizes Caf1, which in turn binds the LRR domain of Ccr4 and tethers the Ccr4 nuclease domain.
Title: Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction.

The following CCR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00798	NM_001178166.1 Latest version!	Saccharomyces cerevisiae S288C CCR4-NOT core exoribonuclease subunit CCR4 (CCR4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCR4 ( NM_001178166.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009381.1 Saccharomyces cerevisiae S288C CCR4-NOT core exoribonuclease subunit CCR4 (CCR4), partial mRNA.

Price & Availability↑

CloneID	OSi00798
Clone ID Related Accession (Same CDS sequence)	NM_001178166.1
Accession Version	NM_001178166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	CCR4-NOT core exoribonuclease subunit CCR4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACGACC CTTCTTTACT AGGCTACCCT AATGTGGGCC CGCAGCAGCA ACAGCAACAG 
CAGCAACAGC AGCATGCCGG GCTACTAGGA AAGGGTACAC CAAACGCTCT ACAGCAGCAG 
CTACACATGA ACCAGCTCAC CGGGATACCT CCACCGGGAC TCATGAACAA CAGCGATGTA 
CACACTTCCA GCAACAACAA CTCGCGCCAG TTGCTCGACC AATTGGCTAA TGGGAACGCG 
AACATGCTCA ATATGAACAT GGATAACAAC AATAACAACA ATAACAACAA CAACAACAAC 
AACAACAACG GCGGTGGAAG CGGGGTGATG ATGAATGCCT CCACAGCCGC AGTAAATTCT 
ATAGGGATGG TTCCTACCGT TGGTACGCCT GTGAACATCA ATGTGAACGC CAGTAACCCT 
TTATTACACC CGCACTTGGA TGACCCTTCT TTACTAAACA ATCCGATCTG GAAGCTTCAA 
TTGCATCTGG CTGCAGTGTC CGCACAATCT CTGGGGCAAC CCAACATTTA TGCTAGACAA 
AACGCTATGA AGAAGTATTT GGCTACACAA CAGGCTCAGC AAGCTCAGCA ACAAGCGCAG 
CAACAGGCAC AGCAGCAGGT CCCGGGCCCA TTTGGCCCCG GACCTCAGGC TGCACCACCA 
GCTTTGCAGC CCACCGATTT CCAGCAATCT CACATTGCAG AAGCCTCCAA ATCACTGGTA 
GACTGCACAA AGCAAGCCTT GATGGAAATG GCCGACACTC TCACCGACAG CAAGACAGCA 
AAGAAACAAC AACCTACGGG AGATAGCACT CCCTCAGGCA CGGCAACTAA CAGTGCAGTT 
TCTACACCAT TGACTCCCAA GATAGAGCTG TTTGCTAATG GCAAGGACGA AGCCAACCAG 
GCGCTCTTAC AACACAAGAA ACTGTCTCAG TACAGCATCG ACGAAGATGA CGACATTGAA 
AACAGAATGG TCATGCCCAA GGACTCGAAA TACGACGACC AATTATGGCA CGCGCTAGAT 
TTGTCCAACT TGCAAATCTT CAATATCAGC GCCAACATCT TCAAGTACGA TTTTCTAACG 
AGACTATATT TGAATGGCAA TAGCCTCACG GAACTGCCAG CGGAGATCAA GAACCTAAGC 
AACCTACGCG TTTTGGACCT GTCGCATAAT AGGTTAACAT CTCTACCAGC GGAACTAGGC 
TCATGTTTCC AATTGAAATA CTTCTACTTT TTTGATAACA TGGTCACCAC ATTACCATGG 
GAGTTTGGGA ACCTGTGTAA CCTTCAGTTT CTTGGTGTAG AAGGAAACCC CTTAGAAAAA 
CAGTTTTTAA AGATCCTTAC AGAAAAATCT GTCACGGGAT TGATTTTCTA CCTAAGGGAC 
AACAGACCAG AGATTCCCTT ACCGCATGAA CGTAGGTTCA TCGAAATCAA TACCGATGGG 
GAACCACAGA GGGAGTACGA TTCTTTGCAG CAATCCACTG AGCATCTGGC CACCGATTTA 
GCCAAGAGGA CGTTCACCGT CTTATCCTAC AACACCTTAT GTCAACACTA TGCCACCCCA 
AAAATGTACC GTTACACACC GTCGTGGGCG TTAAGTTGGG ATTACAGGCG CAATAAATTA 
AAGGAGCAGA TTCTCTCGTA CGACAGTGAT CTGTTGTGTT TACAAGAAGT GGAGTCTAAG 
ACTTTTGAAG AGTATTGGGT GCCCCTATTG GACAAGCACG GTTATACAGG CATCTTCCAT 
GCAAAGGCAA GAGCCAAGAC CATGCATTCC AAGGACTCCA AGAAAGTGGA CGGGTGTTGC 
ATTTTTTTCA AAAGGGACCA ATTCAAGTTG ATCACCAAAG ACGCCATGGA TTTCAGCGGT 
GCTTGGATGA AACACAAGAA GTTCCAAAGA ACTGAAGATT ATTTAAACCG TGCAATGAAC 
AAAGACAACG TTGCACTGTT CTTAAAGCTA CAACACATTC CTAGTGGCGA CACCATATGG 
GCGGTCACCA CGCATTTGCA CTGGGATCCA AAATTTAATG ATGTCAAGAC ATTCCAAGTA 
GGTGTCCTGT TAGATCATCT GGAAACGCTG CTAAAGGAGG AGACATCGCA CAATTTTAGA 
CAGGACATTA AGAAATTTCC TGTGCTCATT TGTGGTGACT TCAATTCATA CATCAACTCC 
GCCGTATACG AATTGATAAA TACAGGCCGT GTCCAAATAC ATCAAGAGGG AAATGGTAGA 
GATTTCGGTT ACATGTCGGA GAAAAATTTC TCACATAACT TGGCTCTCAA ATCAAGTTAT 
AATTGTATCG GAGAACTACC ATTCACCAAT TTCACACCGT CATTCACAGA TGTTATCGAC 
TATATATGGT TTTCTACACA TGCTCTAAGG GTGCGTGGGC TATTGGGTGA AGTGGACCCT 
GAATACGTGA GTAAGTTTAT CGGGTTCCCC AACGACAAAT TCCCCAGTGA CCATATACCA 
TTGTTAGCAA GATTTGAATT TATGAAGACA AACACAGGCA GTAAGAAAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009381.1
CDS	1..2514
Translation	MNDPSLLGYPNVGPQQQQQQQQQQHAGLLGKGTPNALQQQLHMNQLTGIPPPGLMNNSDVHTSSNNNSRQLLDQLANGNANMLNMNMDNNNNNNNNNNNNNNNGGGSGVMMNASTAAVNSIGMVPTVGTPVNINVNASNPLLHPHLDDPSLLNNPIWKLQLHLAAVSAQSLGQPNIYARQNAMKKYLATQQAQQAQQQAQQQAQQQVPGPFGPGPQAAPPALQPTDFQQSHIAEASKSLVDCTKQALMEMADTLTDSKTAKKQQPTGDSTPSGTATNSAVSTPLTPKIELFANGKDEANQALLQHKKLSQYSIDEDDDIENRMVMPKDSKYDDQLWHALDLSNLQIFNISANIFKYDFLTRLYLNGNSLTELPAEIKNLSNLRVLDLSHNRLTSLPAELGSCFQLKYFYFFDNMVTTLPWEFGNLCNLQFLGVEGNPLEKQFLKILTEKSVTGLIFYLRDNRPEIPLPHERRFIEINTDGEPQREYDSLQQSTEHLATDLAKRTFTVLSYNTLCQHYATPKMYRYTPSWALSWDYRRNKLKEQILSYDSDLLCLQEVESKTFEEYWVPLLDKHGYTGIFHAKARAKTMHSKDSKKVDGCCIFFKRDQFKLITKDAMDFSGAWMKHKKFQRTEDYLNRAMNKDNVALFLKLQHIPSGDTIWAVTTHLHWDPKFNDVKTFQVGVLLDHLETLLKEETSHNFRQDIKKFPVLICGDFNSYINSAVYELINTGRVQIHQEGNGRDFGYMSEKNFSHNLALKSSYNCIGELPFTNFTPSFTDVIDYIWFSTHALRVRGLLGEVDPEYVSKFIGFPNDKFPSDHIPLLARFEFMKTNTGSKKV

Target ORF information:

RefSeq Version	NM_001178166.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C CCR4-NOT core exoribonuclease subunit CCR4 (CCR4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178166.1

ORF Insert Sequence:

ATGAACGACC CTTCTTTACT AGGCTACCCT AATGTGGGCC CGCAGCAGCA ACAGCAACAG 
CAGCAACAGC AGCATGCCGG GCTACTAGGA AAGGGTACAC CAAACGCTCT ACAGCAGCAG 
CTACACATGA ACCAGCTCAC CGGGATACCT CCACCGGGAC TCATGAACAA CAGCGATGTA 
CACACTTCCA GCAACAACAA CTCGCGCCAG TTGCTCGACC AATTGGCTAA TGGGAACGCG 
AACATGCTCA ATATGAACAT GGATAACAAC AATAACAACA ATAACAACAA CAACAACAAC 
AACAACAACG GCGGTGGAAG CGGGGTGATG ATGAATGCCT CCACAGCCGC AGTAAATTCT 
ATAGGGATGG TTCCTACCGT TGGTACGCCT GTGAACATCA ATGTGAACGC CAGTAACCCT 
TTATTACACC CGCACTTGGA TGACCCTTCT TTACTAAACA ATCCGATCTG GAAGCTTCAA 
TTGCATCTGG CTGCAGTGTC CGCACAATCT CTGGGGCAAC CCAACATTTA TGCTAGACAA 
AACGCTATGA AGAAGTATTT GGCTACACAA CAGGCTCAGC AAGCTCAGCA ACAAGCGCAG 
CAACAGGCAC AGCAGCAGGT CCCGGGCCCA TTTGGCCCCG GACCTCAGGC TGCACCACCA 
GCTTTGCAGC CCACCGATTT CCAGCAATCT CACATTGCAG AAGCCTCCAA ATCACTGGTA 
GACTGCACAA AGCAAGCCTT GATGGAAATG GCCGACACTC TCACCGACAG CAAGACAGCA 
AAGAAACAAC AACCTACGGG AGATAGCACT CCCTCAGGCA CGGCAACTAA CAGTGCAGTT 
TCTACACCAT TGACTCCCAA GATAGAGCTG TTTGCTAATG GCAAGGACGA AGCCAACCAG 
GCGCTCTTAC AACACAAGAA ACTGTCTCAG TACAGCATCG ACGAAGATGA CGACATTGAA 
AACAGAATGG TCATGCCCAA GGACTCGAAA TACGACGACC AATTATGGCA CGCGCTAGAT 
TTGTCCAACT TGCAAATCTT CAATATCAGC GCCAACATCT TCAAGTACGA TTTTCTAACG 
AGACTATATT TGAATGGCAA TAGCCTCACG GAACTGCCAG CGGAGATCAA GAACCTAAGC 
AACCTACGCG TTTTGGACCT GTCGCATAAT AGGTTAACAT CTCTACCAGC GGAACTAGGC 
TCATGTTTCC AATTGAAATA CTTCTACTTT TTTGATAACA TGGTCACCAC ATTACCATGG 
GAGTTTGGGA ACCTGTGTAA CCTTCAGTTT CTTGGTGTAG AAGGAAACCC CTTAGAAAAA 
CAGTTTTTAA AGATCCTTAC AGAAAAATCT GTCACGGGAT TGATTTTCTA CCTAAGGGAC 
AACAGACCAG AGATTCCCTT ACCGCATGAA CGTAGGTTCA TCGAAATCAA TACCGATGGG 
GAACCACAGA GGGAGTACGA TTCTTTGCAG CAATCCACTG AGCATCTGGC CACCGATTTA 
GCCAAGAGGA CGTTCACCGT CTTATCCTAC AACACCTTAT GTCAACACTA TGCCACCCCA 
AAAATGTACC GTTACACACC GTCGTGGGCG TTAAGTTGGG ATTACAGGCG CAATAAATTA 
AAGGAGCAGA TTCTCTCGTA CGACAGTGAT CTGTTGTGTT TACAAGAAGT GGAGTCTAAG 
ACTTTTGAAG AGTATTGGGT GCCCCTATTG GACAAGCACG GTTATACAGG CATCTTCCAT 
GCAAAGGCAA GAGCCAAGAC CATGCATTCC AAGGACTCCA AGAAAGTGGA CGGGTGTTGC 
ATTTTTTTCA AAAGGGACCA ATTCAAGTTG ATCACCAAAG ACGCCATGGA TTTCAGCGGT 
GCTTGGATGA AACACAAGAA GTTCCAAAGA ACTGAAGATT ATTTAAACCG TGCAATGAAC 
AAAGACAACG TTGCACTGTT CTTAAAGCTA CAACACATTC CTAGTGGCGA CACCATATGG 
GCGGTCACCA CGCATTTGCA CTGGGATCCA AAATTTAATG ATGTCAAGAC ATTCCAAGTA 
GGTGTCCTGT TAGATCATCT GGAAACGCTG CTAAAGGAGG AGACATCGCA CAATTTTAGA 
CAGGACATTA AGAAATTTCC TGTGCTCATT TGTGGTGACT TCAATTCATA CATCAACTCC 
GCCGTATACG AATTGATAAA TACAGGCCGT GTCCAAATAC ATCAAGAGGG AAATGGTAGA 
GATTTCGGTT ACATGTCGGA GAAAAATTTC TCACATAACT TGGCTCTCAA ATCAAGTTAT 
AATTGTATCG GAGAACTACC ATTCACCAAT TTCACACCGT CATTCACAGA TGTTATCGAC 
TATATATGGT TTTCTACACA TGCTCTAAGG GTGCGTGGGC TATTGGGTGA AGTGGACCCT 
GAATACGTGA GTAAGTTTAT CGGGTTCCCC AACGACAAAT TCCCCAGTGA CCATATACCA 
TTGTTAGCAA GATTTGAATT TATGAAGACA AACACAGGCA GTAAGAAAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB

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CCR4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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