C15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	C15
Entrez Gene ID	42544
Full Name	CG7937 gene product from transcript CG7937-RA
Synonyms	311,93Bal,CG7937,Cll,Dmel\CG7937,Ect5,Hox11-311,c15,cll
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93E3-93E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057525.3	NP_476873.2	C15

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100493739	XP_002944078.2
Drosophila melanogaster (fruit fly)	C15	NP_476873.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003674	XP_313455.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we find that C15 interacts physically with the Dll activator through contacts between their homeodomain and binds competitively with Dll to adjacent cognate sites on LAE, adding potential new layers of regulation by C15. Lastly, we show that C15 and Bowl activities regulate also rn expression
Title: Tissue-specific enhancer repression through molecular integration of cell signaling inputs.

In the Al-Cll-DNA complex structure, the residues in the extended regions are used not only for the intermolecular contacts between the two homeodomain proteins but also for the sequence-recognition mechanism of DNA by direct interactions.
Title: Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless.

Mutational analysis shows that the number of Smad- and Zen-binding sites is essential for the C15 transcriptional response, and the spatial limits of C15 expression are established through a repression mechanism in the dorsolateral cells of the embryo.
Title: Threshold response of C15 to the Dpp gradient in Drosophila is established by the cumulative effect of Smad and Zen activators and negative cues.

Data show that Aristaless and Clawless proteins form a complex capable of binding to specific DNA targets, which cannot be well recognized solely by Aristaless or Clawless.
Title: A concerted action of a paired-type homeobox gene, aristaless, and a homolog of Hox11/tlx homeobox gene, clawless, is essential for the distal tip development of the Drosophila leg.

Body patterning of legs is a product of this gene.
Title: Regulation of gene expression in the distal region of the Drosophila leg by the Hox11 homolog, C15.

The following C15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05604	NM_057525.3 Latest version!	Drosophila melanogaster C15 (C15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C15 ( NM_057525.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476873.2 Drosophila melanogaster C15 (C15), mRNA.

Price & Availability↑

CloneID	OFa05604
Clone ID Related Accession (Same CDS sequence)	NM_057525.3
Accession Version	NM_057525.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	C15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057525.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCC ACGAGGAGGA TGACCATGAG CACGAGAACG AGCACGGCGA AGAGGTGGAG 
GAACAGGAGA TCCACGTGGA TGTGGACTCG GACTCGCGGA TGTCATGTGG CAGCGGATCC 
GATGTGGATA TGGACGGCGG CAGCTGCTAC GACGAGTCCG AAACGCCATT GAGCGAGTCC 
CTGCAGTCGG AGCAGACGCG CTCCTCGTCC AGTGAGAATC TGCCCTTCAG CATATCACGC 
CTGCTATCCA AGCCCTTCGA GACGAGTCAC CACCACCACA ACAACAACAA CCACCTGCTC 
AGCAGCAGTC CGGGCTCCAG CAGCAACAAC AACAACAGCG GCGAAAAGGG CGAGGAGAAG 
GAGCTACTGC AGCAGGAGGA CCACGACCTG GCCTATAAGC TGGCCACCAG CATCGCCAAC 
TCGACATACG GAAGCGCCGC AGCACTATAC TCGTATCCGC ATCTCTATCC CTCAGCGGCC 
GGCGGTCATG TGCTACGTGT ACCGCCCCAG AGAACGCCGC TCACCTGGGC ACTACCGCCA 
CTGCATCACG CGGCGCTGGC ACATCAGGCG GTCAAGGATC GGTTGGCAGC TGCCTTTCCG 
ATCGCCCGAC GGATCGGACA TCCCTACCAG AATCGCACGC CGCCGAAGAG GAAAAAGCCA 
CGCACATCCT TCACGCGCAT CCAGGTGGCC GAGTTGGAGA AGCGCTTCCA CAAGCAAAAG 
TATCTGGCAT CCGCGGAGCG AGCGGCACTG GCCCGCGGAC TGAAGATGAC CGATGCCCAG 
GTGAAGACGT GGTTCCAGAA CCGACGCACC AAGTGGAGAC GACAGACGGC CGAGGAGCGC 
GAGGCGGAGC GGCAGGCGGC GAACAGACTG ATGCTGTCCC TCCAGGCGGA GGCCATCAGC 
AAGGGATTCG CGCCTCCTTC GGCACCCCTG GGTTCCCAGG GGGGCGTGAA CGGGGCACCG 
CTGGCTGCGT TGCACGGACT ACAGCCGTGG GCAGAGGCGT CGCATGCGGC GGGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476873.2
CDS	273..1292
Translation	MSSHEEDDHEHENEHGEEVEEQEIHVDVDSDSRMSCGSGSDVDMDGGSCYDESETPLSESLQSEQTRSSSSENLPFSISRLLSKPFETSHHHHNNNNHLLSSSPGSSSNNNNSGEKGEEKELLQQEDHDLAYKLATSIANSTYGSAAALYSYPHLYPSAAGGHVLRVPPQRTPLTWALPPLHHAALAHQAVKDRLAAAFPIARRIGHPYQNRTPPKRKKPRTSFTRIQVAELEKRFHKQKYLASAERAALARGLKMTDAQVKTWFQNRRTKWRRQTAEEREAERQAANRLMLSLQAEAISKGFAPPSAPLGSQGGVNGAPLAALHGLQPWAEASHAAGC

Target ORF information:

RefSeq Version	NM_057525.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster C15 (C15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057525.3

ORF Insert Sequence:

ATGTCCAGCC ACGAGGAGGA TGACCATGAG CACGAGAACG AGCACGGCGA AGAGGTGGAG 
GAACAGGAGA TCCACGTGGA TGTGGACTCG GACTCGCGGA TGTCATGTGG CAGCGGATCC 
GATGTGGATA TGGACGGCGG CAGCTGCTAC GACGAGTCCG AAACGCCATT GAGCGAGTCC 
CTGCAGTCGG AGCAGACGCG CTCCTCGTCC AGTGAGAATC TGCCCTTCAG CATATCACGC 
CTGCTATCCA AGCCCTTCGA GACGAGTCAC CACCACCACA ACAACAACAA CCACCTGCTC 
AGCAGCAGTC CGGGCTCCAG CAGCAACAAC AACAACAGCG GCGAAAAGGG CGAGGAGAAG 
GAGCTACTGC AGCAGGAGGA CCACGACCTG GCCTATAAGC TGGCCACCAG CATCGCCAAC 
TCGACATACG GAAGCGCCGC AGCACTATAC TCGTATCCGC ATCTCTATCC CTCAGCGGCC 
GGCGGTCATG TGCTACGTGT ACCGCCCCAG AGAACGCCGC TCACCTGGGC ACTACCGCCA 
CTGCATCACG CGGCGCTGGC ACATCAGGCG GTCAAGGATC GGTTGGCAGC TGCCTTTCCG 
ATCGCCCGAC GGATCGGACA TCCCTACCAG AATCGCACGC CGCCGAAGAG GAAAAAGCCA 
CGCACATCCT TCACGCGCAT CCAGGTGGCC GAGTTGGAGA AGCGCTTCCA CAAGCAAAAG 
TATCTGGCAT CCGCGGAGCG AGCGGCACTG GCCCGCGGAC TGAAGATGAC CGATGCCCAG 
GTGAAGACGT GGTTCCAGAA CCGACGCACC AAGTGGAGAC GACAGACGGC CGAGGAGCGC 
GAGGCGGAGC GGCAGGCGGC GAACAGACTG ATGCTGTCCC TCCAGGCGGA GGCCATCAGC 
AAGGGATTCG CGCCTCCTTC GGCACCCCTG GGTTCCCAGG GGGGCGTGAA CGGGGCACCG 
CTGGCTGCGT TGCACGGACT ACAGCCGTGG GCAGAGGCGT CGCATGCGGC GGGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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C15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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