Nelf-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelf-A
Entrez Gene ID	42520
Full Name	Negative elongation factor A
Synonyms	BcDNA:SD07139,CG5874,Dmel\CG5874,NELF,NELF-A
General protein information	Preferred Names Negative elongation factor A Names CG5874 gene product from transcript CG5874-RB CG5874-PA CG5874-PB CG5874-PC NELF-A Nelf-A-PA Nelf-A-PB Nelf-A-PC negative elongation factor A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93C7-93C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300509.1	NP_001287438.1	negative elongation factor A, isoform C
NM_142701.2	NP_650958.2	negative elongation factor A, isoform A
NM_001275875.1	NP_001262804.1	negative elongation factor A, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The finding that both phenotypes of arrested embryos are obtained in embryos that lack maternally provided NELF-A as well as in embryos with reduced levels of maternal NELF-E show that these phenotypes result from the reduced activity of the NELF complex.
Title: NELF potentiates gene transcription in the Drosophila embryo.

analysis of the interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex
Title: Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex.

The following Nelf-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelf-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05582	NM_142701.2 Latest version!	Drosophila melanogaster negative elongation factor A, transcript variant A (Nelf-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa08625	NM_001275875.1 Latest version!	Drosophila melanogaster negative elongation factor A, transcript variant B (Nelf-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa22129	NM_001300509.1 Latest version!	Drosophila melanogaster negative elongation factor A, transcript variant C (Nelf-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelf-A ( NM_142701.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650958.2 Drosophila melanogaster negative elongation factor A, transcript variant A (Nelf-A), mRNA.

Price & Availability↑

CloneID	OFa05582
Clone ID Related Accession (Same CDS sequence)	NM_142701.2
Accession Version	NM_142701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	negative elongation factor A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Apr 11, 2005 this sequence version replaced NM_142701.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAACG TAAGGGACAG CGACACATCA CTGTGGCTGC ATAATAAACT GGGCACCTCG 
AACGACTCGT GGATAAACGG CTCCATCTGC TCGCAGCTGA ACAAGGAGGT GCTGCGCAAC 
ATCAAGGAAT GCTTCCCCGA TCTGCAAACG CAGGTCAAAC TGAAGCTCCT ACTCAGCTTC 
CTTCACATCC CGCGCCGTCT GGTGGAGGAG TGGAAGGCGG AACTTGAGGA GGTGATCGAG 
GTAGCCGGCC TGGATTCAGA GCTATGGGTT TCCATGCTGG CGGAGACGAT GAAGACCTTT 
CCGGCGACCA GTTCGCTCAA CACCGAGATC TCCGATTACG AGGACACGCG TCCCATCTTC 
ATAGACATGG TCAACGACCT GCGCAAGCTG GTCACAAAGC ATTCGGACCT CGGCATGCTG 
CCTCTGGAGT GCCAGTACCT AAATAAAAAC GCACTGATCT CAGTGGTGGG CCAGCAACCC 
GCTCCCGTCA AGCATTTTAC GCTGAAGCGC AAGCCGAAGA GCGCCCAGTT GCGCACCGAA 
TTGTTACACA AGTCAGCCGA TGCGCAGTCC TCGCTGAAGA AGGCCTCGGC CCCCACAATA 
CCGCTGCGAT CGCGTGGCAT GCCACGTAAG ATGACAGACA CTACCCCGCT GAAGGGAATC 
CCATCTCGCA TGCCCACCAC TGGGTTCCGT TCGGCCACCG TGCCGGGAAA TGCCGCCCAG 
CGCCCCAATC TCAGTCGAAC GCCTGCTGGA CGCAAAGACG GTGGCATTAA ACTGATCGAA 
TTCACCGAAC AGCCCTTGGG TTATGCGGCG GCTAAGAAGC GAAAGCGCGA GCAGCAACTG 
GAAGAGCAGC AAAAGAAGCA GGAGCAGAAA CAGCAACAGG CAGCTGCAGC GGCAGCTGCG 
GCCGCCACAG CAGCGGCAAG CAATGCTGGT GACAGCTCAC CAACGGACGC GGCGACTGCC 
AGTGGAGGCG AAACACCCGT CACGCCCACG TCAGCTAACA ACAGCTTCGA AATCAAAATG 
GAACCGCAGC AACTCAACCA GAGCGCTGGA AGCCTGGAAG AGCCGCAAGA CGATGAGATG 
TCTGGCAAAG CGAATATGGA ATGCGATATC GAGACGCCGG AGTACGCTAC AGCCACGCTG 
GACTTTGCCC AGGCCACTTC GACGTCCGTG GCGGATGGTC AACCGGGAAA ACCAGCAGTA 
GAAACGAAGC TGAAGACGCC GCGAACTCCG AAATCCGCAG CAAAACTGAA CAACAACAAC 
AACAACAACA ACAACAACAG CTTCAATCAC ACCCCAAAAC GAATCAAGCA GGAAATAGAG 
ATCAAGAGCG AGGAGATCAT AGTACCCGCC AGCATCAAAC TCGAAAAGAT CGAAACTTCA 
CCGTCGACCC AACGCGTCAG TATCCAGCAA CAGCCGCCTA GTTTGGTGCA GCGCACCCCG 
CACCTGCTTA TCCGCAGTTC GCCGCAGAAG CGGCAGAACA ACGGGGCAAC CACGAGTGCA 
GGCACAACCA CAACCACTGT GGGCAATACC ACCATCAAGA TGGAGAAGCT GGACATCAAG 
CCAATGATAC GCGCCACCGG TGTCTCGCCT TCGACGAGTG CGGGCACTAC GACTACACTT 
CTTACCCCGC AGCAACTGCG TCAGGCGGCC AATCCGTTGG CCAATCTGCC CAACAATATC 
TCCGTGAAGA TAACCTCTGC CAAGGCGAAA GCGGCAGCTG CAGCAGCGGC AACATCCAGT 
GGCAGCCAGA CTCAGTCGCA GCAGCCGCAA ACGTTGCAGG TTCAGCAGGC GCCTCAGCAG 
TCGCATCCCC CGCTTTTGAT TAACAGCTCC ACACCCGTGA TCTTGGCGTC CTCGCCCAGC 
GCTCAGAGAG CGAAGGCTTT GGCTTTGCCT AGTAGCAGTA CCTCCGCCAC AACTACCACG 
ACGATACCGT CGCAAGCCAT AAAGACGATG CCACTGAGCC AGCTGAAGAC GGCTACCAAC 
AGCGGACCAG TGATCATATC GCAGACTATT ATCCAGCCGG CAAAGCGAGC CCAACAACAG 
GCTGGAACCT CCAGTGCAGC GGCCGCTTCG CAACAGCAGC AGCAACAACA GTCGCAGCAG 
CAATTGTTAC ACCAGCAAAT CCAGCAGCAG ACCCAACAGC AGGCGCAGTT ACCACAGCAG 
CCTCAGCAGC AACAGACGCA GTATATTCTA GCCACACCCC AGCAGCAACC GCAACAAATT 
CAACAGCAGC AACAACAGCA ACAGCCCATT TTGCCAACGC TTACCTCGTT CTCACACAGT 
CGGCCTATGC CGCAGACTAC GACGCTTTAC CAAGCCACTA CTCCTAGCGG AAGCGGTCAG 
ACGCCGACCA AGATTTTGCT AAAGACATCG GGCTCTTCAA GCGTGGTGAT GACGCCTCTG 
CGCCAAGCGC AGCCGCAACA GGCCACCGCT GTGGTGTCGT CGAATCCTCC GCCATTGGTG 
GCCACTTCGG CGGCAGTAGT TTCCCCGGGT CAGACAACCC TGAACATTCA GAATGTGCAG 
CTACCCAATC GACCAGTGAC CATCCAACCA GCCTCCCAAG CCGCCCAGCA GCAGCATATG 
CAGGCCCAGT TGCAGCAGCA GCAACCGCAT CCACAGCACA CGATCGTTGC CAATACGACG 
GCCACGCAGC AGCCCAAGTT GTCTCAGGTT CTAATGCAGC CAACTGCAGC AGTTGGCACC 
AGTGGTGTAT CCGCTCTGAA CGTATCCCCT ACGTCCGGAA AGAACAAGAC CATCATACTC 
ACCCAGAAGG GCGTTATACT GCGCAACATT GGCGGCGATA TGTACCAGCA AATACCAATC 
AGCAATGTGG GCAACATGCA GGGACTCGGA GGCACCACGC TGATGACAAC CACAGCTGGG 
CCGCCGAGTC TGGTGAAGAC AACACCCTCG AGCGGTGTAC AGTTGCAGCA GCAACAGTCT 
GGAAAGCAGA TCCTGCCCAC TCTCATACCA ACCAGCTCGC TTGGAGGACA GCACGTTATT 
GTGCAGCAGC AGCAACCCAC CAATGTCATC GGGAATTCCC AACAGCAGAC CATCATTCGG 
CCCGTTATGA CCAACGTGGG CGGAGGCTTG ACCACGCTGC CGCAGGGCCT GACGCTAATT 
CAACGGCCGG GCCAGCAGCC GCAGCTGGTG CAGGTGCAGG CGGCGCCGGG CAGCACGCAG 
CGCACGATTA TTACCCAATC GAACACGGCG GCGGCGGCGT CGCAACAGCA GCCGCGCCAG 
CAGCAGCAAC AAATCCTGGT CCAACACAAG CCGGCTCCGA CCCTGCAGCA GCGTTTGGTC 
ACCTCCACCA CCAGCGGTGG ACAGGGACAG CAGGGAAATC CGAATGCGGG GCTCCCGCGT 
ACCGTGCAGG TGCAGGTGCA AGCTCAGCAG CAGCAGCAAC CACAGCAGCA GCAAGCTACG 
CAACAGCAAT CGCAGCAGGC ACCCCAGCGA CGTGGACTCT CTCTATCGAA CGAACACGTG 
CACAAGGCGC ACGAAATGTT CCGCAAGGCA AATCGCGTCT CGCGACCTGA CAAAGCCCTT 
ATCCTTGGAT TTATGGCGGG TCTGCGGGAA AACCCGCGCC CAAATAACGA AAACGTGCTG 
GTCATCAAGT TGGGCGAAAC AGAGGAAAAA GTACAACAGG ACAACGGCCA CACGGCACTG 
TGCCTGGTTG AGTCACACAT CCGGCTGGAC TACAACACCG GTGAGTGGAA GACATTCCAA 
AACTACAGGC TGCAGGACCA GAGTGCCGCC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650958.2
CDS	167..3922
Translation	MANVRDSDTSLWLHNKLGTSNDSWINGSICSQLNKEVLRNIKECFPDLQTQVKLKLLLSFLHIPRRLVEEWKAELEEVIEVAGLDSELWVSMLAETMKTFPATSSLNTEISDYEDTRPIFIDMVNDLRKLVTKHSDLGMLPLECQYLNKNALISVVGQQPAPVKHFTLKRKPKSAQLRTELLHKSADAQSSLKKASAPTIPLRSRGMPRKMTDTTPLKGIPSRMPTTGFRSATVPGNAAQRPNLSRTPAGRKDGGIKLIEFTEQPLGYAAAKKRKREQQLEEQQKKQEQKQQQAAAAAAAAATAAASNAGDSSPTDAATASGGETPVTPTSANNSFEIKMEPQQLNQSAGSLEEPQDDEMSGKANMECDIETPEYATATLDFAQATSTSVADGQPGKPAVETKLKTPRTPKSAAKLNNNNNNNNNNSFNHTPKRIKQEIEIKSEEIIVPASIKLEKIETSPSTQRVSIQQQPPSLVQRTPHLLIRSSPQKRQNNGATTSAGTTTTTVGNTTIKMEKLDIKPMIRATGVSPSTSAGTTTTLLTPQQLRQAANPLANLPNNISVKITSAKAKAAAAAAATSSGSQTQSQQPQTLQVQQAPQQSHPPLLINSSTPVILASSPSAQRAKALALPSSSTSATTTTTIPSQAIKTMPLSQLKTATNSGPVIISQTIIQPAKRAQQQAGTSSAAAASQQQQQQQSQQQLLHQQIQQQTQQQAQLPQQPQQQQTQYILATPQQQPQQIQQQQQQQQPILPTLTSFSHSRPMPQTTTLYQATTPSGSGQTPTKILLKTSGSSSVVMTPLRQAQPQQATAVVSSNPPPLVATSAAVVSPGQTTLNIQNVQLPNRPVTIQPASQAAQQQHMQAQLQQQQPHPQHTIVANTTATQQPKLSQVLMQPTAAVGTSGVSALNVSPTSGKNKTIILTQKGVILRNIGGDMYQQIPISNVGNMQGLGGTTLMTTTAGPPSLVKTTPSSGVQLQQQQSGKQILPTLIPTSSLGGQHVIVQQQQPTNVIGNSQQQTIIRPVMTNVGGGLTTLPQGLTLIQRPGQQPQLVQVQAAPGSTQRTIITQSNTAAAASQQQPRQQQQQILVQHKPAPTLQQRLVTSTTSGGQGQQGNPNAGLPRTVQVQVQAQQQQQPQQQQATQQQSQQAPQRRGLSLSNEHVHKAHEMFRKANRVSRPDKALILGFMAGLRENPRPNNENVLVIKLGETEEKVQQDNGHTALCLVESHIRLDYNTGEWKTFQNYRLQDQSAAS

Target ORF information:

RefSeq Version	NM_142701.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster negative elongation factor A, transcript variant A (Nelf-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142701.2

ORF Insert Sequence:

ATGGCGAACG TAAGGGACAG CGACACATCA CTGTGGCTGC ATAATAAACT GGGCACCTCG 
AACGACTCGT GGATAAACGG CTCCATCTGC TCGCAGCTGA ACAAGGAGGT GCTGCGCAAC 
ATCAAGGAAT GCTTCCCCGA TCTGCAAACG CAGGTCAAAC TGAAGCTCCT ACTCAGCTTC 
CTTCACATCC CGCGCCGTCT GGTGGAGGAG TGGAAGGCGG AACTTGAGGA GGTGATCGAG 
GTAGCCGGCC TGGATTCAGA GCTATGGGTT TCCATGCTGG CGGAGACGAT GAAGACCTTT 
CCGGCGACCA GTTCGCTCAA CACCGAGATC TCCGATTACG AGGACACGCG TCCCATCTTC 
ATAGACATGG TCAACGACCT GCGCAAGCTG GTCACAAAGC ATTCGGACCT CGGCATGCTG 
CCTCTGGAGT GCCAGTACCT AAATAAAAAC GCACTGATCT CAGTGGTGGG CCAGCAACCC 
GCTCCCGTCA AGCATTTTAC GCTGAAGCGC AAGCCGAAGA GCGCCCAGTT GCGCACCGAA 
TTGTTACACA AGTCAGCCGA TGCGCAGTCC TCGCTGAAGA AGGCCTCGGC CCCCACAATA 
CCGCTGCGAT CGCGTGGCAT GCCACGTAAG ATGACAGACA CTACCCCGCT GAAGGGAATC 
CCATCTCGCA TGCCCACCAC TGGGTTCCGT TCGGCCACCG TGCCGGGAAA TGCCGCCCAG 
CGCCCCAATC TCAGTCGAAC GCCTGCTGGA CGCAAAGACG GTGGCATTAA ACTGATCGAA 
TTCACCGAAC AGCCCTTGGG TTATGCGGCG GCTAAGAAGC GAAAGCGCGA GCAGCAACTG 
GAAGAGCAGC AAAAGAAGCA GGAGCAGAAA CAGCAACAGG CAGCTGCAGC GGCAGCTGCG 
GCCGCCACAG CAGCGGCAAG CAATGCTGGT GACAGCTCAC CAACGGACGC GGCGACTGCC 
AGTGGAGGCG AAACACCCGT CACGCCCACG TCAGCTAACA ACAGCTTCGA AATCAAAATG 
GAACCGCAGC AACTCAACCA GAGCGCTGGA AGCCTGGAAG AGCCGCAAGA CGATGAGATG 
TCTGGCAAAG CGAATATGGA ATGCGATATC GAGACGCCGG AGTACGCTAC AGCCACGCTG 
GACTTTGCCC AGGCCACTTC GACGTCCGTG GCGGATGGTC AACCGGGAAA ACCAGCAGTA 
GAAACGAAGC TGAAGACGCC GCGAACTCCG AAATCCGCAG CAAAACTGAA CAACAACAAC 
AACAACAACA ACAACAACAG CTTCAATCAC ACCCCAAAAC GAATCAAGCA GGAAATAGAG 
ATCAAGAGCG AGGAGATCAT AGTACCCGCC AGCATCAAAC TCGAAAAGAT CGAAACTTCA 
CCGTCGACCC AACGCGTCAG TATCCAGCAA CAGCCGCCTA GTTTGGTGCA GCGCACCCCG 
CACCTGCTTA TCCGCAGTTC GCCGCAGAAG CGGCAGAACA ACGGGGCAAC CACGAGTGCA 
GGCACAACCA CAACCACTGT GGGCAATACC ACCATCAAGA TGGAGAAGCT GGACATCAAG 
CCAATGATAC GCGCCACCGG TGTCTCGCCT TCGACGAGTG CGGGCACTAC GACTACACTT 
CTTACCCCGC AGCAACTGCG TCAGGCGGCC AATCCGTTGG CCAATCTGCC CAACAATATC 
TCCGTGAAGA TAACCTCTGC CAAGGCGAAA GCGGCAGCTG CAGCAGCGGC AACATCCAGT 
GGCAGCCAGA CTCAGTCGCA GCAGCCGCAA ACGTTGCAGG TTCAGCAGGC GCCTCAGCAG 
TCGCATCCCC CGCTTTTGAT TAACAGCTCC ACACCCGTGA TCTTGGCGTC CTCGCCCAGC 
GCTCAGAGAG CGAAGGCTTT GGCTTTGCCT AGTAGCAGTA CCTCCGCCAC AACTACCACG 
ACGATACCGT CGCAAGCCAT AAAGACGATG CCACTGAGCC AGCTGAAGAC GGCTACCAAC 
AGCGGACCAG TGATCATATC GCAGACTATT ATCCAGCCGG CAAAGCGAGC CCAACAACAG 
GCTGGAACCT CCAGTGCAGC GGCCGCTTCG CAACAGCAGC AGCAACAACA GTCGCAGCAG 
CAATTGTTAC ACCAGCAAAT CCAGCAGCAG ACCCAACAGC AGGCGCAGTT ACCACAGCAG 
CCTCAGCAGC AACAGACGCA GTATATTCTA GCCACACCCC AGCAGCAACC GCAACAAATT 
CAACAGCAGC AACAACAGCA ACAGCCCATT TTGCCAACGC TTACCTCGTT CTCACACAGT 
CGGCCTATGC CGCAGACTAC GACGCTTTAC CAAGCCACTA CTCCTAGCGG AAGCGGTCAG 
ACGCCGACCA AGATTTTGCT AAAGACATCG GGCTCTTCAA GCGTGGTGAT GACGCCTCTG 
CGCCAAGCGC AGCCGCAACA GGCCACCGCT GTGGTGTCGT CGAATCCTCC GCCATTGGTG 
GCCACTTCGG CGGCAGTAGT TTCCCCGGGT CAGACAACCC TGAACATTCA GAATGTGCAG 
CTACCCAATC GACCAGTGAC CATCCAACCA GCCTCCCAAG CCGCCCAGCA GCAGCATATG 
CAGGCCCAGT TGCAGCAGCA GCAACCGCAT CCACAGCACA CGATCGTTGC CAATACGACG 
GCCACGCAGC AGCCCAAGTT GTCTCAGGTT CTAATGCAGC CAACTGCAGC AGTTGGCACC 
AGTGGTGTAT CCGCTCTGAA CGTATCCCCT ACGTCCGGAA AGAACAAGAC CATCATACTC 
ACCCAGAAGG GCGTTATACT GCGCAACATT GGCGGCGATA TGTACCAGCA AATACCAATC 
AGCAATGTGG GCAACATGCA GGGACTCGGA GGCACCACGC TGATGACAAC CACAGCTGGG 
CCGCCGAGTC TGGTGAAGAC AACACCCTCG AGCGGTGTAC AGTTGCAGCA GCAACAGTCT 
GGAAAGCAGA TCCTGCCCAC TCTCATACCA ACCAGCTCGC TTGGAGGACA GCACGTTATT 
GTGCAGCAGC AGCAACCCAC CAATGTCATC GGGAATTCCC AACAGCAGAC CATCATTCGG 
CCCGTTATGA CCAACGTGGG CGGAGGCTTG ACCACGCTGC CGCAGGGCCT GACGCTAATT 
CAACGGCCGG GCCAGCAGCC GCAGCTGGTG CAGGTGCAGG CGGCGCCGGG CAGCACGCAG 
CGCACGATTA TTACCCAATC GAACACGGCG GCGGCGGCGT CGCAACAGCA GCCGCGCCAG 
CAGCAGCAAC AAATCCTGGT CCAACACAAG CCGGCTCCGA CCCTGCAGCA GCGTTTGGTC 
ACCTCCACCA CCAGCGGTGG ACAGGGACAG CAGGGAAATC CGAATGCGGG GCTCCCGCGT 
ACCGTGCAGG TGCAGGTGCA AGCTCAGCAG CAGCAGCAAC CACAGCAGCA GCAAGCTACG 
CAACAGCAAT CGCAGCAGGC ACCCCAGCGA CGTGGACTCT CTCTATCGAA CGAACACGTG 
CACAAGGCGC ACGAAATGTT CCGCAAGGCA AATCGCGTCT CGCGACCTGA CAAAGCCCTT 
ATCCTTGGAT TTATGGCGGG TCTGCGGGAA AACCCGCGCC CAAATAACGA AAACGTGCTG 
GTCATCAAGT TGGGCGAAAC AGAGGAAAAA GTACAACAGG ACAACGGCCA CACGGCACTG 
TGCCTGGTTG AGTCACACAT CCGGCTGGAC TACAACACCG GTGAGTGGAA GACATTCCAA 
AACTACAGGC TGCAGGACCA GAGTGCCGCC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelf-A ( NM_001275875.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262804.1 Drosophila melanogaster negative elongation factor A, transcript variant B (Nelf-A), mRNA.

Price & Availability↑

CloneID	OFa08625
Clone ID Related Accession (Same CDS sequence)	NM_001275875.1
Accession Version	NM_001275875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	negative elongation factor A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAACG TAAGGGACAG CGACACATCA CTGTGGCTGC ATAATAAACT GGGCACCTCG 
AACGACTCGT GGATAAACGG CTCCATCTGC TCGCAGCTGA ACAAGGAGGT GCTGCGCAAC 
ATCAAGGAAT GCTTCCCCGA TCTGCAAACG CAGGTCAAAC TGAAGCTCCT ACTCAGCTTC 
CTTCACATCC CGCGCCGTCT GGTGGAGGAG TGGAAGGCGG AACTTGAGGA GGTGATCGAG 
GTAGCCGGCC TGGATTCAGA GCTATGGGTT TCCATGCTGG CGGAGACGAT GAAGACCTTT 
CCGGCGACCA GTTCGCTCAA CACCGAGATC TCCGATTACG AGGACACGCG TCCCATCTTC 
ATAGACATGG TCAACGACCT GCGCAAGCTG GTCACAAAGC ATTCGGACCT CGGCATGCTG 
CCTCTGGAGT GCCAGTACCT AAATAAAAAC GCACTGATCT CAGTGGTGGG CCAGCAACCC 
GCTCCCGTCA AGCATTTTAC GCTGAAGCGC AAGCCGAAGA GCGCCCAGTT GCGCACCGAA 
TTGTTACACA AGTCAGCCGA TGCGCAGTCC TCGCTGAAGA AGGCCTCGGC CCCCACAATA 
CCGCTGCGAT CGCGTGGCAT GCCACGTAAG ATGACAGACA CTACCCCGCT GAAGGGAATC 
CCATCTCGCA TGCCCACCAC TGGGTTCCGT TCGGCCACCG TGCCGGGAAA TGCCGCCCAG 
CGCCCCAATC TCAGTCGAAC GCCTGCTGGA CGCAAAGACG GTGGCATTAA ACTGATCGAA 
TTCACCGAAC AGCCCTTGGG TTATGCGGCG GCTAAGAAGC GAAAGCGCGA GCAGCAACTG 
GAAGAGCAGC AAAAGAAGCA GGAGCAGAAA CAGCAACAGG CAGCTGCAGC GGCAGCTGCG 
GCCGCCACAG CAGCGGCAAG CAATGCTGGT GACAGCTCAC CAACGGACGC GGCGACTGCC 
AGTGGAGGCG AAACACCCGT CACGCCCACG TCAGCTAACA ACAGCTTCGA AATCAAAATG 
GAACCGCAGC AACTCAACCA GAGCGCTGGA AGCCTGGAAG AGCCGCAAGA CGATGAGATG 
TCTGGCAAAG CGAATATGGA ATGCGATATC GAGACGCCGG AGTACGCTAC AGCCACGCTG 
GACTTTGCCC AGGCCACTTC GACGTCCGTG GCGGATGGTC AACCGGGAAA ACCAGCAGTA 
GAAACGAAGC TGAAGACGCC GCGAACTCCG AAATCCGCAG CAAAACTGAA CAACAACAAC 
AACAACAACA ACAACAACAG CTTCAATCAC ACCCCAAAAC GAATCAAGCA GGAAATAGAG 
ATCAAGAGCG AGGAGATCAT AGTACCCGCC AGCATCAAAC TCGAAAAGAT CGAAACTTCA 
CCGTCGACCC AACGCGTCAG TATCCAGCAA CAGCCGCCTA GTTTGGTGCA GCGCACCCCG 
CACCTGCTTA TCCGCAGTTC GCCGCAGAAG CGGCAGAACA ACGGGGCAAC CACGAGTGCA 
GGCACAACCA CAACCACTGT GGGCAATACC ACCATCAAGA TGGAGAAGCT GGACATCAAG 
CCAATGATAC GCGCCACCGG TGTCTCGCCT TCGACGAGTG CGGGCACTAC GACTACACTT 
CTTACCCCGC AGCAACTGCG TCAGGCGGCC AATCCGTTGG CCAATCTGCC CAACAATATC 
TCCGTGAAGA TAACCTCTGC CAAGGCGAAA GCGGCAGCTG CAGCAGCGGC AACATCCAGT 
GGCAGCCAGA CTCAGTCGCA GCAGCCGCAA ACGTTGCAGG TTCAGCAGGC GCCTCAGCAG 
TCGCATCCCC CGCTTTTGAT TAACAGCTCC ACACCCGTGA TCTTGGCGTC CTCGCCCAGC 
GCTCAGAGAG CGAAGGCTTT GGCTTTGCCT AGTAGCAGTA CCTCCGCCAC AACTACCACG 
ACGATACCGT CGCAAGCCAT AAAGACGATG CCACTGAGCC AGCTGAAGAC GGCTACCAAC 
AGCGGACCAG TGATCATATC GCAGACTATT ATCCAGCCGG CAAAGCGAGC CCAACAACAG 
GCTGGAACCT CCAGTGCAGC GGCCGCTTCG CAACAGCAGC AGCAACAACA GTCGCAGCAG 
CAATTGTTAC ACCAGCAAAT CCAGCAGCAG ACCCAACAGC AGGCGCAGTT ACCACAGCAG 
CCTCAGCAGC AACAGACGCA GTATATTCTA GCCACACCCC AGCAGCAACC GCAACAAATT 
CAACAGCAGC AACAACAGCA ACAGCCCATT TTGCCAACGC TTACCTCGTT CTCACACAGT 
CGGCCTATGC CGCAGACTAC GACGCTTTAC CAAGCCACTA CTCCTAGCGG AAGCGGTCAG 
ACGCCGACCA AGATTTTGCT AAAGACATCG GGCTCTTCAA GCGTGGTGAT GACGCCTCTG 
CGCCAAGCGC AGCCGCAACA GGCCACCGCT GTGGTGTCGT CGAATCCTCC GCCATTGGTG 
GCCACTTCGG CGGCAGTAGT TTCCCCGGGT CAGACAACCC TGAACATTCA GAATGTGCAG 
CTACCCAATC GACCAGTGAC CATCCAACCA GCCTCCCAAG CCGCCCAGCA GCAGCATATG 
CAGGCCCAGT TGCAGCAGCA GCAACCGCAT CCACAGCACA CGATCGTTGC CAATACGACG 
GCCACGCAGC AGCCCAAGTT GTCTCAGGTT CTAATGCAGC CAACTGCAGC AGTTGGCACC 
AGTGGTGTAT CCGCTCTGAA CGTATCCCCT ACGTCCGGAA AGAACAAGAC CATCATACTC 
ACCCAGAAGG GCGTTATACT GCGCAACATT GGCGGCGATA TGTACCAGCA AATACCAATC 
AGCAATGTGG GCAACATGCA GGGACTCGGA GGCACCACGC TGATGACAAC CACAGCTGGG 
CCGCCGAGTC TGGTGAAGAC AACACCCTCG AGCGGTGTAC AGTTGCAGCA GCAACAGTCT 
GGAAAGCAGA TCCTGCCCAC TCTCATACCA ACCAGCTCGC TTGGAGGACA GCACGTTATT 
GTGCAGCAGC AGCAACCCAC CAATGTCATC GGGAATTCCC AACAGCAGAC CATCATTCGG 
CCCGTTATGA CCAACGTGGG CGGAGGCTTG ACCACGCTGC CGCAGGGCCT GACGCTAATT 
CAACGGCCGG GCCAGCAGCC GCAGCTGGTG CAGGTGCAGG CGGCGCCGGG CAGCACGCAG 
CGCACGATTA TTACCCAATC GAACACGGCG GCGGCGGCGT CGCAACAGCA GCCGCGCCAG 
CAGCAGCAAC AAATCCTGGT CCAACACAAG CCGGCTCCGA CCCTGCAGCA GCGTTTGGTC 
ACCTCCACCA CCAGCGGTGG ACAGGGACAG CAGGGAAATC CGAATGCGGG GCTCCCGCGT 
ACCGTGCAGG TGCAGGTGCA AGCTCAGCAG CAGCAGCAAC CACAGCAGCA GCAAGCTACG 
CAACAGCAAT CGCAGCAGGC ACCCCAGCGA CGTGGACTCT CTCTATCGAA CGAACACGTG 
CACAAGGCGC ACGAAATGTT CCGCAAGGCA AATCGCGTCT CGCGACCTGA CAAAGCCCTT 
ATCCTTGGAT TTATGGCGGG TCTGCGGGAA AACCCGCGCC CAAATAACGA AAACGTGCTG 
GTCATCAAGT TGGGCGAAAC AGAGGAAAAA GTACAACAGG ACAACGGCCA CACGGCACTG 
TGCCTGGTTG AGTCACACAT CCGGCTGGAC TACAACACCG GTGAGTGGAA GACATTCCAA 
AACTACAGGC TGCAGGACCA GAGTGCCGCC TCATAGTCCA CAAGGGTCAT ACGCATAGTT 
CGCAGCAACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262804.1
CDS	167..3961
Translation	MANVRDSDTSLWLHNKLGTSNDSWINGSICSQLNKEVLRNIKECFPDLQTQVKLKLLLSFLHIPRRLVEEWKAELEEVIEVAGLDSELWVSMLAETMKTFPATSSLNTEISDYEDTRPIFIDMVNDLRKLVTKHSDLGMLPLECQYLNKNALISVVGQQPAPVKHFTLKRKPKSAQLRTELLHKSADAQSSLKKASAPTIPLRSRGMPRKMTDTTPLKGIPSRMPTTGFRSATVPGNAAQRPNLSRTPAGRKDGGIKLIEFTEQPLGYAAAKKRKREQQLEEQQKKQEQKQQQAAAAAAAAATAAASNAGDSSPTDAATASGGETPVTPTSANNSFEIKMEPQQLNQSAGSLEEPQDDEMSGKANMECDIETPEYATATLDFAQATSTSVADGQPGKPAVETKLKTPRTPKSAAKLNNNNNNNNNNSFNHTPKRIKQEIEIKSEEIIVPASIKLEKIETSPSTQRVSIQQQPPSLVQRTPHLLIRSSPQKRQNNGATTSAGTTTTTVGNTTIKMEKLDIKPMIRATGVSPSTSAGTTTTLLTPQQLRQAANPLANLPNNISVKITSAKAKAAAAAAATSSGSQTQSQQPQTLQVQQAPQQSHPPLLINSSTPVILASSPSAQRAKALALPSSSTSATTTTTIPSQAIKTMPLSQLKTATNSGPVIISQTIIQPAKRAQQQAGTSSAAAASQQQQQQQSQQQLLHQQIQQQTQQQAQLPQQPQQQQTQYILATPQQQPQQIQQQQQQQQPILPTLTSFSHSRPMPQTTTLYQATTPSGSGQTPTKILLKTSGSSSVVMTPLRQAQPQQATAVVSSNPPPLVATSAAVVSPGQTTLNIQNVQLPNRPVTIQPASQAAQQQHMQAQLQQQQPHPQHTIVANTTATQQPKLSQVLMQPTAAVGTSGVSALNVSPTSGKNKTIILTQKGVILRNIGGDMYQQIPISNVGNMQGLGGTTLMTTTAGPPSLVKTTPSSGVQLQQQQSGKQILPTLIPTSSLGGQHVIVQQQQPTNVIGNSQQQTIIRPVMTNVGGGLTTLPQGLTLIQRPGQQPQLVQVQAAPGSTQRTIITQSNTAAAASQQQPRQQQQQILVQHKPAPTLQQRLVTSTTSGGQGQQGNPNAGLPRTVQVQVQAQQQQQPQQQQATQQQSQQAPQRRGLSLSNEHVHKAHEMFRKANRVSRPDKALILGFMAGLRENPRPNNENVLVIKLGETEEKVQQDNGHTALCLVESHIRLDYNTGEWKTFQNYRLQDQSAAS*STRVIRIVRSNK

Target ORF information:

RefSeq Version	NM_001275875.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster negative elongation factor A, transcript variant B (Nelf-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275875.1

ORF Insert Sequence:

ATGGCGAACG TAAGGGACAG CGACACATCA CTGTGGCTGC ATAATAAACT GGGCACCTCG 
AACGACTCGT GGATAAACGG CTCCATCTGC TCGCAGCTGA ACAAGGAGGT GCTGCGCAAC 
ATCAAGGAAT GCTTCCCCGA TCTGCAAACG CAGGTCAAAC TGAAGCTCCT ACTCAGCTTC 
CTTCACATCC CGCGCCGTCT GGTGGAGGAG TGGAAGGCGG AACTTGAGGA GGTGATCGAG 
GTAGCCGGCC TGGATTCAGA GCTATGGGTT TCCATGCTGG CGGAGACGAT GAAGACCTTT 
CCGGCGACCA GTTCGCTCAA CACCGAGATC TCCGATTACG AGGACACGCG TCCCATCTTC 
ATAGACATGG TCAACGACCT GCGCAAGCTG GTCACAAAGC ATTCGGACCT CGGCATGCTG 
CCTCTGGAGT GCCAGTACCT AAATAAAAAC GCACTGATCT CAGTGGTGGG CCAGCAACCC 
GCTCCCGTCA AGCATTTTAC GCTGAAGCGC AAGCCGAAGA GCGCCCAGTT GCGCACCGAA 
TTGTTACACA AGTCAGCCGA TGCGCAGTCC TCGCTGAAGA AGGCCTCGGC CCCCACAATA 
CCGCTGCGAT CGCGTGGCAT GCCACGTAAG ATGACAGACA CTACCCCGCT GAAGGGAATC 
CCATCTCGCA TGCCCACCAC TGGGTTCCGT TCGGCCACCG TGCCGGGAAA TGCCGCCCAG 
CGCCCCAATC TCAGTCGAAC GCCTGCTGGA CGCAAAGACG GTGGCATTAA ACTGATCGAA 
TTCACCGAAC AGCCCTTGGG TTATGCGGCG GCTAAGAAGC GAAAGCGCGA GCAGCAACTG 
GAAGAGCAGC AAAAGAAGCA GGAGCAGAAA CAGCAACAGG CAGCTGCAGC GGCAGCTGCG 
GCCGCCACAG CAGCGGCAAG CAATGCTGGT GACAGCTCAC CAACGGACGC GGCGACTGCC 
AGTGGAGGCG AAACACCCGT CACGCCCACG TCAGCTAACA ACAGCTTCGA AATCAAAATG 
GAACCGCAGC AACTCAACCA GAGCGCTGGA AGCCTGGAAG AGCCGCAAGA CGATGAGATG 
TCTGGCAAAG CGAATATGGA ATGCGATATC GAGACGCCGG AGTACGCTAC AGCCACGCTG 
GACTTTGCCC AGGCCACTTC GACGTCCGTG GCGGATGGTC AACCGGGAAA ACCAGCAGTA 
GAAACGAAGC TGAAGACGCC GCGAACTCCG AAATCCGCAG CAAAACTGAA CAACAACAAC 
AACAACAACA ACAACAACAG CTTCAATCAC ACCCCAAAAC GAATCAAGCA GGAAATAGAG 
ATCAAGAGCG AGGAGATCAT AGTACCCGCC AGCATCAAAC TCGAAAAGAT CGAAACTTCA 
CCGTCGACCC AACGCGTCAG TATCCAGCAA CAGCCGCCTA GTTTGGTGCA GCGCACCCCG 
CACCTGCTTA TCCGCAGTTC GCCGCAGAAG CGGCAGAACA ACGGGGCAAC CACGAGTGCA 
GGCACAACCA CAACCACTGT GGGCAATACC ACCATCAAGA TGGAGAAGCT GGACATCAAG 
CCAATGATAC GCGCCACCGG TGTCTCGCCT TCGACGAGTG CGGGCACTAC GACTACACTT 
CTTACCCCGC AGCAACTGCG TCAGGCGGCC AATCCGTTGG CCAATCTGCC CAACAATATC 
TCCGTGAAGA TAACCTCTGC CAAGGCGAAA GCGGCAGCTG CAGCAGCGGC AACATCCAGT 
GGCAGCCAGA CTCAGTCGCA GCAGCCGCAA ACGTTGCAGG TTCAGCAGGC GCCTCAGCAG 
TCGCATCCCC CGCTTTTGAT TAACAGCTCC ACACCCGTGA TCTTGGCGTC CTCGCCCAGC 
GCTCAGAGAG CGAAGGCTTT GGCTTTGCCT AGTAGCAGTA CCTCCGCCAC AACTACCACG 
ACGATACCGT CGCAAGCCAT AAAGACGATG CCACTGAGCC AGCTGAAGAC GGCTACCAAC 
AGCGGACCAG TGATCATATC GCAGACTATT ATCCAGCCGG CAAAGCGAGC CCAACAACAG 
GCTGGAACCT CCAGTGCAGC GGCCGCTTCG CAACAGCAGC AGCAACAACA GTCGCAGCAG 
CAATTGTTAC ACCAGCAAAT CCAGCAGCAG ACCCAACAGC AGGCGCAGTT ACCACAGCAG 
CCTCAGCAGC AACAGACGCA GTATATTCTA GCCACACCCC AGCAGCAACC GCAACAAATT 
CAACAGCAGC AACAACAGCA ACAGCCCATT TTGCCAACGC TTACCTCGTT CTCACACAGT 
CGGCCTATGC CGCAGACTAC GACGCTTTAC CAAGCCACTA CTCCTAGCGG AAGCGGTCAG 
ACGCCGACCA AGATTTTGCT AAAGACATCG GGCTCTTCAA GCGTGGTGAT GACGCCTCTG 
CGCCAAGCGC AGCCGCAACA GGCCACCGCT GTGGTGTCGT CGAATCCTCC GCCATTGGTG 
GCCACTTCGG CGGCAGTAGT TTCCCCGGGT CAGACAACCC TGAACATTCA GAATGTGCAG 
CTACCCAATC GACCAGTGAC CATCCAACCA GCCTCCCAAG CCGCCCAGCA GCAGCATATG 
CAGGCCCAGT TGCAGCAGCA GCAACCGCAT CCACAGCACA CGATCGTTGC CAATACGACG 
GCCACGCAGC AGCCCAAGTT GTCTCAGGTT CTAATGCAGC CAACTGCAGC AGTTGGCACC 
AGTGGTGTAT CCGCTCTGAA CGTATCCCCT ACGTCCGGAA AGAACAAGAC CATCATACTC 
ACCCAGAAGG GCGTTATACT GCGCAACATT GGCGGCGATA TGTACCAGCA AATACCAATC 
AGCAATGTGG GCAACATGCA GGGACTCGGA GGCACCACGC TGATGACAAC CACAGCTGGG 
CCGCCGAGTC TGGTGAAGAC AACACCCTCG AGCGGTGTAC AGTTGCAGCA GCAACAGTCT 
GGAAAGCAGA TCCTGCCCAC TCTCATACCA ACCAGCTCGC TTGGAGGACA GCACGTTATT 
GTGCAGCAGC AGCAACCCAC CAATGTCATC GGGAATTCCC AACAGCAGAC CATCATTCGG 
CCCGTTATGA CCAACGTGGG CGGAGGCTTG ACCACGCTGC CGCAGGGCCT GACGCTAATT 
CAACGGCCGG GCCAGCAGCC GCAGCTGGTG CAGGTGCAGG CGGCGCCGGG CAGCACGCAG 
CGCACGATTA TTACCCAATC GAACACGGCG GCGGCGGCGT CGCAACAGCA GCCGCGCCAG 
CAGCAGCAAC AAATCCTGGT CCAACACAAG CCGGCTCCGA CCCTGCAGCA GCGTTTGGTC 
ACCTCCACCA CCAGCGGTGG ACAGGGACAG CAGGGAAATC CGAATGCGGG GCTCCCGCGT 
ACCGTGCAGG TGCAGGTGCA AGCTCAGCAG CAGCAGCAAC CACAGCAGCA GCAAGCTACG 
CAACAGCAAT CGCAGCAGGC ACCCCAGCGA CGTGGACTCT CTCTATCGAA CGAACACGTG 
CACAAGGCGC ACGAAATGTT CCGCAAGGCA AATCGCGTCT CGCGACCTGA CAAAGCCCTT 
ATCCTTGGAT TTATGGCGGG TCTGCGGGAA AACCCGCGCC CAAATAACGA AAACGTGCTG 
GTCATCAAGT TGGGCGAAAC AGAGGAAAAA GTACAACAGG ACAACGGCCA CACGGCACTG 
TGCCTGGTTG AGTCACACAT CCGGCTGGAC TACAACACCG GTGAGTGGAA GACATTCCAA 
AACTACAGGC TGCAGGACCA GAGTGCCGCC TCATAGTCCA CAAGGGTCAT ACGCATAGTT 
CGCAGCAACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelf-A ( NM_001300509.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287438.1 Drosophila melanogaster negative elongation factor A, transcript variant C (Nelf-A), mRNA.

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CloneID	OFa22129
Clone ID Related Accession (Same CDS sequence)	NM_001300509.1
Accession Version	NM_001300509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	negative elongation factor A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAACG TAAGGGACAG CGACACATCA CTGTGGCTGC ATAATAAACT GGGCACCTCG 
AACGACTCGT GGATAAACGG CTCCATCTGC TCGCAGCTGA ACAAGGAGGT GCTGCGCAAC 
ATCAAGGAAT GCTTCCCCGA TCTGCAAACG CAGGTCAAAC TGAAGCTCCT ACTCAGCTTC 
CTTCACATCC CGCGCCGTCT GGTGGAGGAG TGGAAGGCGG AACTTGAGGA GGTGATCGAG 
GTAGCCGGCC TGGATTCAGA GCTATGGGTT TCCATGCTGG CGGAGACGAT GAAGACCTTT 
CCGGCGACCA GTTCGCTCAA CACCGAGATC TCCGATTACG AGGACACGCG TCCCATCTTC 
ATAGACATGG TCAACGACCT GCGCAAGCTG GTCACAAAGC ATTCGGACCT CGGCATGCTG 
CCTCTGGAGT GCCAGTACCT AAATAAAAAC GCACTGATCT CAGTGGTGGG CCAGCAACCC 
GCTCCCGTCA AGCATTTTAC GCTGAAGCGC AAGCCGAAGA GCGCCCAGTT GCGCACCGAA 
TTGTTACACA AGTCAGCCGA TGCGCAGTCC TCGCTGAAGA AGGCCTCGGC CCCCACAATA 
CCGCTGCGAT CGCGTGGCAT GCCACGTAAG ATGACAGACA CTACCCCGCT GAAGGGAATC 
CCATCTCGCA TGCCCACCAC TGGGTTCCGT TCGGCCACCG TGCCGGGAAA TGCCGCCCAG 
CGCCCCAATC TCAGTCGAAC GCCTGCTGGA CGCAAAGACG GTGGCATTAA ACTGATCGAA 
TTCACCGAAC AGCCCTTGGG TTATGCGGCG GCTAAGAAGC GAAAGCGCGA GCAGCAACTG 
GAAGAGCAGC AAAAGAAGCA GGAGCAGAAA CAGCAACAGG CAGCTGCAGC GGCAGCTGCG 
GCCGCCACAG CAGCGGCAAG CAATGCTGGT GACAGCTCAC CAACGGACGC GGCGACTGCC 
AGTGGAGGCG AAACACCCGT CACGCCCACG TCAGCTAACA ACAGCTTCGA AATCAAAATG 
GAACCGCAGC AACTCAACCA GAGCGCTGGA AGCCTGGAAG AGCCGCAAGA CGATGAGATG 
TCTGGCAAAG CGAATATGGA ATGCGATATC GAGACGCCGG AGTACGCTAC AGCCACGCTG 
GACTTTGCCC AGGCCACTTC GACGTCCGTG GCGGATGGTC AACCGGGAAA ACCAGCAGTA 
GAAACGAAGC TGAAGACGCC GCGAACTCCG AAATCCGCAG CAAAACTGAA CAACAACAAC 
AACAACAACA ACAACAACAG CTTCAATCAC ACCCCAAAAC GAATCAAGCA GGAAATAGAG 
ATCAAGAGCG AGGAGATCAT AGTACCCGCC AGCATCAAAC TCGAAAAGAT CGAAACTTCA 
CCGTCGACCC AACGCGTCAG TATCCAGCAA CAGCCGCCTA GTTTGGTGCA GCGCACCCCG 
CACCTGCTTA TCCGCAGTTC GCCGCAGAAG CGGCAGAACA ACGGGGCAAC CACGAGTGCA 
GGCACAACCA CAACCACTGT GGGCAATACC ACCATCAAGA TGGAGAAGCT GGACATCAAG 
CCAATGATAC GCGCCACCGG TGTCTCGCCT TCGACGAGTG CGGGCACTAC GACTACACTT 
CTTACCCCGC AGCAACTGCG TCAGGCGGCC AATCCGTTGG CCAATCTGCC CAACAATATC 
TCCGTGAAGA TAACCTCTGC CAAGGCGAAA GCGGCAGCTG CAGCAGCGGC AACATCCAGT 
GGCAGCCAGA CTCAGTCGCA GCAGCCGCAA ACGTTGCAGG TTCAGCAGGC GCCTCAGCAG 
TCGCATCCCC CGCTTTTGAT TAACAGCTCC ACACCCGTGA TCTTGGCGTC CTCGCCCAGC 
GCTCAGAGAG CGGCTTTGGC TTTGCCTAGT AGCAGTACCT CCGCCACAAC TACCACGACG 
ATACCGTCGC AAGCCATAAA GACGATGCCA CTGAGCCAGC TGAAGACGGC TACCAACAGC 
GGACCAGTGA TCATATCGCA GACTATTATC CAGCCGGCAA AGCGAGCCCA ACAACAGGCT 
GGAACCTCCA GTGCAGCGGC CGCTTCGCAA CAGCAGCAGC AACAACAGTC GCAGCAGCAA 
TTGTTACACC AGCAAATCCA GCAGCAGACC CAACAGCAGG CGCAGTTACC ACAGCAGCCT 
CAGCAGCAAC AGACGCAGTA TATTCTAGCC ACACCCCAGC AGCAACCGCA ACAAATTCAA 
CAGCAGCAAC AACAGCAACA GCCCATTTTG CCAACGCTTA CCTCGTTCTC ACACAGTCGG 
CCTATGCCGC AGACTACGAC GCTTTACCAA GCCACTACTC CTAGCGGAAG CGGTCAGACG 
CCGACCAAGA TTTTGCTAAA GACATCGGGC TCTTCAAGCG TGGTGATGAC GCCTCTGCGC 
CAAGCGCAGC CGCAACAGGC CACCGCTGTG GTGTCGTCGA ATCCTCCGCC ATTGGTGGCC 
ACTTCGGCGG CAGTAGTTTC CCCGGGTCAG ACAACCCTGA ACATTCAGAA TGTGCAGCTA 
CCCAATCGAC CAGTGACCAT CCAACCAGCC TCCCAAGCCG CCCAGCAGCA GCATATGCAG 
GCCCAGTTGC AGCAGCAGCA ACCGCATCCA CAGCACACGA TCGTTGCCAA TACGACGGCC 
ACGCAGCAGC CCAAGTTGTC TCAGGTTCTA ATGCAGCCAA CTGCAGCAGT TGGCACCAGT 
GGTGTATCCG CTCTGAACGT ATCCCCTACG TCCGGAAAGA ACAAGACCAT CATACTCACC 
CAGAAGGGCG TTATACTGCG CAACATTGGC GGCGATATGT ACCAGCAAAT ACCAATCAGC 
AATGTGGGCA ACATGCAGGG ACTCGGAGGC ACCACGCTGA TGACAACCAC AGCTGGGCCG 
CCGAGTCTGG TGAAGACAAC ACCCTCGAGC GGTGTACAGT TGCAGCAGCA ACAGTCTGGA 
AAGCAGATCC TGCCCACTCT CATACCAACC AGCTCGCTTG GAGGACAGCA CGTTATTGTG 
CAGCAGCAGC AACCCACCAA TGTCATCGGG AATTCCCAAC AGCAGACCAT CATTCGGCCC 
GTTATGACCA ACGTGGGCGG AGGCTTGACC ACGCTGCCGC AGGGCCTGAC GCTAATTCAA 
CGGCCGGGCC AGCAGCCGCA GCTGGTGCAG GTGCAGGCGG CGCCGGGCAG CACGCAGCGC 
ACGATTATTA CCCAATCGAA CACGGCGGCG GCGGCGTCGC AACAGCAGCC GCGCCAGCAG 
CAGCAACAAA TCCTGGTCCA ACACAAGCCG GCTCCGACCC TGCAGCAGCG TTTGGTCACC 
TCCACCACCA GCGGTGGACA GGGACAGCAG GGAAATCCGA ATGCGGGGCT CCCGCGTACC 
GTGCAGGTGC AGGTGCAAGC TCAGCAGCAG CAGCAACCAC AGCAGCAGCA AGCTACGCAA 
CAGCAATCGC AGCAGGCACC CCAGCGACGT GGACTCTCTC TATCGAACGA ACACGTGCAC 
AAGGCGCACG AAATGTTCCG CAAGGCAAAT CGCGTCTCGC GACCTGACAA AGCCCTTATC 
CTTGGATTTA TGGCGGGTCT GCGGGAAAAC CCGCGCCCAA ATAACGAAAA CGTGCTGGTC 
ATCAAGTTGG GCGAAACAGA GGAAAAAGTA CAACAGGACA ACGGCCACAC GGCACTGTGC 
CTGGTTGAGT CACACATCCG GCTGGACTAC AACACCGGTG AGTGGAAGAC ATTCCAAAAC 
TACAGGCTGC AGGACCAGAG TGCCGCCTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287438.1
CDS	167..3919
Translation	MANVRDSDTSLWLHNKLGTSNDSWINGSICSQLNKEVLRNIKECFPDLQTQVKLKLLLSFLHIPRRLVEEWKAELEEVIEVAGLDSELWVSMLAETMKTFPATSSLNTEISDYEDTRPIFIDMVNDLRKLVTKHSDLGMLPLECQYLNKNALISVVGQQPAPVKHFTLKRKPKSAQLRTELLHKSADAQSSLKKASAPTIPLRSRGMPRKMTDTTPLKGIPSRMPTTGFRSATVPGNAAQRPNLSRTPAGRKDGGIKLIEFTEQPLGYAAAKKRKREQQLEEQQKKQEQKQQQAAAAAAAAATAAASNAGDSSPTDAATASGGETPVTPTSANNSFEIKMEPQQLNQSAGSLEEPQDDEMSGKANMECDIETPEYATATLDFAQATSTSVADGQPGKPAVETKLKTPRTPKSAAKLNNNNNNNNNNSFNHTPKRIKQEIEIKSEEIIVPASIKLEKIETSPSTQRVSIQQQPPSLVQRTPHLLIRSSPQKRQNNGATTSAGTTTTTVGNTTIKMEKLDIKPMIRATGVSPSTSAGTTTTLLTPQQLRQAANPLANLPNNISVKITSAKAKAAAAAAATSSGSQTQSQQPQTLQVQQAPQQSHPPLLINSSTPVILASSPSAQRAALALPSSSTSATTTTTIPSQAIKTMPLSQLKTATNSGPVIISQTIIQPAKRAQQQAGTSSAAAASQQQQQQQSQQQLLHQQIQQQTQQQAQLPQQPQQQQTQYILATPQQQPQQIQQQQQQQQPILPTLTSFSHSRPMPQTTTLYQATTPSGSGQTPTKILLKTSGSSSVVMTPLRQAQPQQATAVVSSNPPPLVATSAAVVSPGQTTLNIQNVQLPNRPVTIQPASQAAQQQHMQAQLQQQQPHPQHTIVANTTATQQPKLSQVLMQPTAAVGTSGVSALNVSPTSGKNKTIILTQKGVILRNIGGDMYQQIPISNVGNMQGLGGTTLMTTTAGPPSLVKTTPSSGVQLQQQQSGKQILPTLIPTSSLGGQHVIVQQQQPTNVIGNSQQQTIIRPVMTNVGGGLTTLPQGLTLIQRPGQQPQLVQVQAAPGSTQRTIITQSNTAAAASQQQPRQQQQQILVQHKPAPTLQQRLVTSTTSGGQGQQGNPNAGLPRTVQVQVQAQQQQQPQQQQATQQQSQQAPQRRGLSLSNEHVHKAHEMFRKANRVSRPDKALILGFMAGLRENPRPNNENVLVIKLGETEEKVQQDNGHTALCLVESHIRLDYNTGEWKTFQNYRLQDQSAAS

Target ORF information:

RefSeq Version	NM_001300509.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster negative elongation factor A, transcript variant C (Nelf-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300509.1

ORF Insert Sequence:

ATGGCGAACG TAAGGGACAG CGACACATCA CTGTGGCTGC ATAATAAACT GGGCACCTCG 
AACGACTCGT GGATAAACGG CTCCATCTGC TCGCAGCTGA ACAAGGAGGT GCTGCGCAAC 
ATCAAGGAAT GCTTCCCCGA TCTGCAAACG CAGGTCAAAC TGAAGCTCCT ACTCAGCTTC 
CTTCACATCC CGCGCCGTCT GGTGGAGGAG TGGAAGGCGG AACTTGAGGA GGTGATCGAG 
GTAGCCGGCC TGGATTCAGA GCTATGGGTT TCCATGCTGG CGGAGACGAT GAAGACCTTT 
CCGGCGACCA GTTCGCTCAA CACCGAGATC TCCGATTACG AGGACACGCG TCCCATCTTC 
ATAGACATGG TCAACGACCT GCGCAAGCTG GTCACAAAGC ATTCGGACCT CGGCATGCTG 
CCTCTGGAGT GCCAGTACCT AAATAAAAAC GCACTGATCT CAGTGGTGGG CCAGCAACCC 
GCTCCCGTCA AGCATTTTAC GCTGAAGCGC AAGCCGAAGA GCGCCCAGTT GCGCACCGAA 
TTGTTACACA AGTCAGCCGA TGCGCAGTCC TCGCTGAAGA AGGCCTCGGC CCCCACAATA 
CCGCTGCGAT CGCGTGGCAT GCCACGTAAG ATGACAGACA CTACCCCGCT GAAGGGAATC 
CCATCTCGCA TGCCCACCAC TGGGTTCCGT TCGGCCACCG TGCCGGGAAA TGCCGCCCAG 
CGCCCCAATC TCAGTCGAAC GCCTGCTGGA CGCAAAGACG GTGGCATTAA ACTGATCGAA 
TTCACCGAAC AGCCCTTGGG TTATGCGGCG GCTAAGAAGC GAAAGCGCGA GCAGCAACTG 
GAAGAGCAGC AAAAGAAGCA GGAGCAGAAA CAGCAACAGG CAGCTGCAGC GGCAGCTGCG 
GCCGCCACAG CAGCGGCAAG CAATGCTGGT GACAGCTCAC CAACGGACGC GGCGACTGCC 
AGTGGAGGCG AAACACCCGT CACGCCCACG TCAGCTAACA ACAGCTTCGA AATCAAAATG 
GAACCGCAGC AACTCAACCA GAGCGCTGGA AGCCTGGAAG AGCCGCAAGA CGATGAGATG 
TCTGGCAAAG CGAATATGGA ATGCGATATC GAGACGCCGG AGTACGCTAC AGCCACGCTG 
GACTTTGCCC AGGCCACTTC GACGTCCGTG GCGGATGGTC AACCGGGAAA ACCAGCAGTA 
GAAACGAAGC TGAAGACGCC GCGAACTCCG AAATCCGCAG CAAAACTGAA CAACAACAAC 
AACAACAACA ACAACAACAG CTTCAATCAC ACCCCAAAAC GAATCAAGCA GGAAATAGAG 
ATCAAGAGCG AGGAGATCAT AGTACCCGCC AGCATCAAAC TCGAAAAGAT CGAAACTTCA 
CCGTCGACCC AACGCGTCAG TATCCAGCAA CAGCCGCCTA GTTTGGTGCA GCGCACCCCG 
CACCTGCTTA TCCGCAGTTC GCCGCAGAAG CGGCAGAACA ACGGGGCAAC CACGAGTGCA 
GGCACAACCA CAACCACTGT GGGCAATACC ACCATCAAGA TGGAGAAGCT GGACATCAAG 
CCAATGATAC GCGCCACCGG TGTCTCGCCT TCGACGAGTG CGGGCACTAC GACTACACTT 
CTTACCCCGC AGCAACTGCG TCAGGCGGCC AATCCGTTGG CCAATCTGCC CAACAATATC 
TCCGTGAAGA TAACCTCTGC CAAGGCGAAA GCGGCAGCTG CAGCAGCGGC AACATCCAGT 
GGCAGCCAGA CTCAGTCGCA GCAGCCGCAA ACGTTGCAGG TTCAGCAGGC GCCTCAGCAG 
TCGCATCCCC CGCTTTTGAT TAACAGCTCC ACACCCGTGA TCTTGGCGTC CTCGCCCAGC 
GCTCAGAGAG CGGCTTTGGC TTTGCCTAGT AGCAGTACCT CCGCCACAAC TACCACGACG 
ATACCGTCGC AAGCCATAAA GACGATGCCA CTGAGCCAGC TGAAGACGGC TACCAACAGC 
GGACCAGTGA TCATATCGCA GACTATTATC CAGCCGGCAA AGCGAGCCCA ACAACAGGCT 
GGAACCTCCA GTGCAGCGGC CGCTTCGCAA CAGCAGCAGC AACAACAGTC GCAGCAGCAA 
TTGTTACACC AGCAAATCCA GCAGCAGACC CAACAGCAGG CGCAGTTACC ACAGCAGCCT 
CAGCAGCAAC AGACGCAGTA TATTCTAGCC ACACCCCAGC AGCAACCGCA ACAAATTCAA 
CAGCAGCAAC AACAGCAACA GCCCATTTTG CCAACGCTTA CCTCGTTCTC ACACAGTCGG 
CCTATGCCGC AGACTACGAC GCTTTACCAA GCCACTACTC CTAGCGGAAG CGGTCAGACG 
CCGACCAAGA TTTTGCTAAA GACATCGGGC TCTTCAAGCG TGGTGATGAC GCCTCTGCGC 
CAAGCGCAGC CGCAACAGGC CACCGCTGTG GTGTCGTCGA ATCCTCCGCC ATTGGTGGCC 
ACTTCGGCGG CAGTAGTTTC CCCGGGTCAG ACAACCCTGA ACATTCAGAA TGTGCAGCTA 
CCCAATCGAC CAGTGACCAT CCAACCAGCC TCCCAAGCCG CCCAGCAGCA GCATATGCAG 
GCCCAGTTGC AGCAGCAGCA ACCGCATCCA CAGCACACGA TCGTTGCCAA TACGACGGCC 
ACGCAGCAGC CCAAGTTGTC TCAGGTTCTA ATGCAGCCAA CTGCAGCAGT TGGCACCAGT 
GGTGTATCCG CTCTGAACGT ATCCCCTACG TCCGGAAAGA ACAAGACCAT CATACTCACC 
CAGAAGGGCG TTATACTGCG CAACATTGGC GGCGATATGT ACCAGCAAAT ACCAATCAGC 
AATGTGGGCA ACATGCAGGG ACTCGGAGGC ACCACGCTGA TGACAACCAC AGCTGGGCCG 
CCGAGTCTGG TGAAGACAAC ACCCTCGAGC GGTGTACAGT TGCAGCAGCA ACAGTCTGGA 
AAGCAGATCC TGCCCACTCT CATACCAACC AGCTCGCTTG GAGGACAGCA CGTTATTGTG 
CAGCAGCAGC AACCCACCAA TGTCATCGGG AATTCCCAAC AGCAGACCAT CATTCGGCCC 
GTTATGACCA ACGTGGGCGG AGGCTTGACC ACGCTGCCGC AGGGCCTGAC GCTAATTCAA 
CGGCCGGGCC AGCAGCCGCA GCTGGTGCAG GTGCAGGCGG CGCCGGGCAG CACGCAGCGC 
ACGATTATTA CCCAATCGAA CACGGCGGCG GCGGCGTCGC AACAGCAGCC GCGCCAGCAG 
CAGCAACAAA TCCTGGTCCA ACACAAGCCG GCTCCGACCC TGCAGCAGCG TTTGGTCACC 
TCCACCACCA GCGGTGGACA GGGACAGCAG GGAAATCCGA ATGCGGGGCT CCCGCGTACC 
GTGCAGGTGC AGGTGCAAGC TCAGCAGCAG CAGCAACCAC AGCAGCAGCA AGCTACGCAA 
CAGCAATCGC AGCAGGCACC CCAGCGACGT GGACTCTCTC TATCGAACGA ACACGTGCAC 
AAGGCGCACG AAATGTTCCG CAAGGCAAAT CGCGTCTCGC GACCTGACAA AGCCCTTATC 
CTTGGATTTA TGGCGGGTCT GCGGGAAAAC CCGCGCCCAA ATAACGAAAA CGTGCTGGTC 
ATCAAGTTGG GCGAAACAGA GGAAAAAGTA CAACAGGACA ACGGCCACAC GGCACTGTGC 
CTGGTTGAGT CACACATCCG GCTGGACTAC AACACCGGTG AGTGGAAGAC ATTCCAAAAC 
TACAGGCTGC AGGACCAGAG TGCCGCCTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nelf-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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