CG5466 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5466
Entrez Gene ID	42444
Full Name	CG5466 gene product from transcript CG5466-RA
Synonyms	Dmel\CG5466
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F3-92F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_142644.2	NP_650901.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5466 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5466 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05493	NM_142644.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG5466), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5466 ( NM_142644.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650901.2 Drosophila melanogaster uncharacterized protein (CG5466), mRNA.

Price & Availability↑

CloneID	OFa05493
Clone ID Related Accession (Same CDS sequence)	NM_142644.2
Accession Version	NM_142644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142644.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACAATT TGGAGCACTC CAAGCATTTC TATCCTGATA ACTACAAATT GGATCTGTCT 
GAGACCATGA AGGCGGCTCT GTCCAAAGGA CCCGGTGGTG GTGCCGGTGG CGGGGGCGTG 
GCAGGCGGAG ATATGCTGCC GCAGCCAAAT GCTGGAATGC CAGGAGTGGG CTATGTTACG 
GAAAAGCTGT ACATGCTGCT GCAGCTGTAT CTGCAGAACA AAGGCTGGAA TCCCAGTGCT 
GAGCTGCTGC AGTGCTTCAG CGATCTCAAG GACAATGCCA TGCTGCCCAG CGCCGCCTAT 
CTGCAAGTCC TGGCCAACCG CTTAACCCTG GACTCCCAGG GACGGCTAAT ACTGCGCGAC 
AATGGAAAGA TTGTCTTGCC CTTTGAGCAT TTTGCCAATG CGGTCATGCT AAAGCACATG 
TCAGGTCCGC ACGGCCTGCA CCTTAGTGTC GATGCCACGG TGAGGGCAGT CATTGAGTCG 
TACACCATCG GTCGGGATCA GTTCGGCATG GAGAAAGAGT TCATCATCGA GGTGGTGCAG 
TCCTGCCCAA GTCCCGCATG TCGCTACTAT AAAAACCACA TGGGAATTTC GCCCATGCCG 
TTCATGGAGC AGCAGTATCC GGGCGTAAAC ACTCCCGAGT TCCTGCAGCG CTTGGCGCAT 
CTGCCGCCTG GGGGAGCAGC CGGATCTGGG CCAGGAGGCT CCGCAGCCGG ATCCACCTCC 
AGCGTGGCAA GCAGCTCGAG TTCGGCGAAC GTGGAAATCG ATCTGTCCAA GTCGACGGGC 
GCGAAGGTGC CCCAGGTGCC GGTGCCGCCG CAGTTGCAGC ACCTGACCAA GCAGCAGCAG 
AGCACTCTCC AGACGCAGCT CTCCCAGATC AGTGCTGCCG CCGCTCATCA TCAACAACAA 
CAACAGCAGC AACAACAGCA ACAGCAGCAG CAGCAGCAAC AACAACAGCA AGCAGCAGCC 
AAGCACCAGC AGCAATTGAC TGCTGCCCTC ATCCAACAGC AAAACCGTGT CCTCGCCCAG 
CAAAATTTAG AGAAGCTTAG CAACCACCTC AGTCCGCTGG AAAAGCAGCG AGTTTTCGCC 
CAGCTGGATC CAAAGCACTT TGATCCCATG GCCGTTGCGG CTGCAGCCGC CAATCTTCAA 
ATCCAAGGCC TGATGCAATC GCAGGCAGTG GCTGCCGCCG TCGCCGCCAA TCAGCAGACT 
CCGGCGGCTA CGACTACATC TGCTGCAGGA GGAGCTGCAG CCGGCCCGCA GGCCCATCAC 
GGACACCATC CCTTGCCACC GCCATCGCAG TCGCCACACT CATCCTCTTC GTCTTCCTCT 
CATTCCTCGT TGGACCAGAT TACCCACAAG AACGTCGCCG ACTCCTTGCG TTCCAATCTG 
GACTCAATAG AATCGAATAA GGATCTGCTG GCGCTGCACA ACGGAGCCTG GGCCACCGGC 
GATGCCAATC GAATGGATCA CTTGGCCGTG GGTCAGGACA AGATAGTGCG CATCTTTGCG 
GAGCTGATGC GAAACATGTC GCGGATGAAA ACCTACATAC GTCCCTCGAT GTGCAAGCCC 
TACGGCAAGC AGAGTGAGAG TCTGCAGAAA ACCCTCATGG ACACCATTCA GATCGTGCAG 
ACGCTGCGCA ACTGCCTCCC AGCTCCTCAC ATACCCGTGT CGTCCTGGAA AAGTGAGAGC 
GAGGGCAACG TGGGATCAGG AGTGAGCGCG GGAGTAGGAG TGGGTGTCCC TGCAGGACTT 
AACCTGGGCG CCGCCTTGGG TCTGCCATCG GGCAACATGC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650901.2
CDS	209..1993
Translation	MNNLEHSKHFYPDNYKLDLSETMKAALSKGPGGGAGGGGVAGGDMLPQPNAGMPGVGYVTEKLYMLLQLYLQNKGWNPSAELLQCFSDLKDNAMLPSAAYLQVLANRLTLDSQGRLILRDNGKIVLPFEHFANAVMLKHMSGPHGLHLSVDATVRAVIESYTIGRDQFGMEKEFIIEVVQSCPSPACRYYKNHMGISPMPFMEQQYPGVNTPEFLQRLAHLPPGGAAGSGPGGSAAGSTSSVASSSSSANVEIDLSKSTGAKVPQVPVPPQLQHLTKQQQSTLQTQLSQISAAAAHHQQQQQQQQQQQQQQQQQQQQAAAKHQQQLTAALIQQQNRVLAQQNLEKLSNHLSPLEKQRVFAQLDPKHFDPMAVAAAAANLQIQGLMQSQAVAAAVAANQQTPAATTTSAAGGAAAGPQAHHGHHPLPPPSQSPHSSSSSSSHSSLDQITHKNVADSLRSNLDSIESNKDLLALHNGAWATGDANRMDHLAVGQDKIVRIFAELMRNMSRMKTYIRPSMCKPYGKQSESLQKTLMDTIQIVQTLRNCLPAPHIPVSSWKSESEGNVGSGVSAGVGVGVPAGLNLGAALGLPSGNMH

Target ORF information:

RefSeq Version	NM_142644.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG5466), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142644.2

ORF Insert Sequence:

ATGAACAATT TGGAGCACTC CAAGCATTTC TATCCTGATA ACTACAAATT GGATCTGTCT 
GAGACCATGA AGGCGGCTCT GTCCAAAGGA CCCGGTGGTG GTGCCGGTGG CGGGGGCGTG 
GCAGGCGGAG ATATGCTGCC GCAGCCAAAT GCTGGAATGC CAGGAGTGGG CTATGTTACG 
GAAAAGCTGT ACATGCTGCT GCAGCTGTAT CTGCAGAACA AAGGCTGGAA TCCCAGTGCT 
GAGCTGCTGC AGTGCTTCAG CGATCTCAAG GACAATGCCA TGCTGCCCAG CGCCGCCTAT 
CTGCAAGTCC TGGCCAACCG CTTAACCCTG GACTCCCAGG GACGGCTAAT ACTGCGCGAC 
AATGGAAAGA TTGTCTTGCC CTTTGAGCAT TTTGCCAATG CGGTCATGCT AAAGCACATG 
TCAGGTCCGC ACGGCCTGCA CCTTAGTGTC GATGCCACGG TGAGGGCAGT CATTGAGTCG 
TACACCATCG GTCGGGATCA GTTCGGCATG GAGAAAGAGT TCATCATCGA GGTGGTGCAG 
TCCTGCCCAA GTCCCGCATG TCGCTACTAT AAAAACCACA TGGGAATTTC GCCCATGCCG 
TTCATGGAGC AGCAGTATCC GGGCGTAAAC ACTCCCGAGT TCCTGCAGCG CTTGGCGCAT 
CTGCCGCCTG GGGGAGCAGC CGGATCTGGG CCAGGAGGCT CCGCAGCCGG ATCCACCTCC 
AGCGTGGCAA GCAGCTCGAG TTCGGCGAAC GTGGAAATCG ATCTGTCCAA GTCGACGGGC 
GCGAAGGTGC CCCAGGTGCC GGTGCCGCCG CAGTTGCAGC ACCTGACCAA GCAGCAGCAG 
AGCACTCTCC AGACGCAGCT CTCCCAGATC AGTGCTGCCG CCGCTCATCA TCAACAACAA 
CAACAGCAGC AACAACAGCA ACAGCAGCAG CAGCAGCAAC AACAACAGCA AGCAGCAGCC 
AAGCACCAGC AGCAATTGAC TGCTGCCCTC ATCCAACAGC AAAACCGTGT CCTCGCCCAG 
CAAAATTTAG AGAAGCTTAG CAACCACCTC AGTCCGCTGG AAAAGCAGCG AGTTTTCGCC 
CAGCTGGATC CAAAGCACTT TGATCCCATG GCCGTTGCGG CTGCAGCCGC CAATCTTCAA 
ATCCAAGGCC TGATGCAATC GCAGGCAGTG GCTGCCGCCG TCGCCGCCAA TCAGCAGACT 
CCGGCGGCTA CGACTACATC TGCTGCAGGA GGAGCTGCAG CCGGCCCGCA GGCCCATCAC 
GGACACCATC CCTTGCCACC GCCATCGCAG TCGCCACACT CATCCTCTTC GTCTTCCTCT 
CATTCCTCGT TGGACCAGAT TACCCACAAG AACGTCGCCG ACTCCTTGCG TTCCAATCTG 
GACTCAATAG AATCGAATAA GGATCTGCTG GCGCTGCACA ACGGAGCCTG GGCCACCGGC 
GATGCCAATC GAATGGATCA CTTGGCCGTG GGTCAGGACA AGATAGTGCG CATCTTTGCG 
GAGCTGATGC GAAACATGTC GCGGATGAAA ACCTACATAC GTCCCTCGAT GTGCAAGCCC 
TACGGCAAGC AGAGTGAGAG TCTGCAGAAA ACCCTCATGG ACACCATTCA GATCGTGCAG 
ACGCTGCGCA ACTGCCTCCC AGCTCCTCAC ATACCCGTGT CGTCCTGGAA AAGTGAGAGC 
GAGGGCAACG TGGGATCAGG AGTGAGCGCG GGAGTAGGAG TGGGTGTCCC TGCAGGACTT 
AACCTGGGCG CCGCCTTGGG TCTGCCATCG GGCAACATGC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5466 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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