tinc cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following tinc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tinc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa05277 NM_169780.5
Latest version!
Drosophila melanogaster tincar (tinc), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa05276 NM_176511.3
Latest version!
Drosophila melanogaster tincar (tinc), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa05276 NM_176510.5
Latest version!
Drosophila melanogaster tincar (tinc), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa05276 NM_001275757.1
Latest version!
Drosophila melanogaster tincar (tinc), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa05276 NM_176509.4
Latest version!
Drosophila melanogaster tincar (tinc), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa05277
    Clone ID Related Accession (Same CDS sequence) NM_169780.5
    Accession Version NM_169780.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product tincar, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169780.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GACCATACGA GCCGTGGAGC CTCGGTATGT TTTGCCAGTG
    CTACGGGGGA AGTTGACGAC GGCGAGTACG CGACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_732278.2
    CDS826..3804
    Translation

    Target ORF information:

    RefSeq Version NM_169780.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster tincar (tinc), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169780.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GACCATACGA GCCGTGGAGC CTCGGTATGT TTTGCCAGTG
    CTACGGGGGA AGTTGACGAC GGCGAGTACG CGACACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05276
    Clone ID Related Accession (Same CDS sequence) NM_176509.4 , NM_176510.5 , NM_176511.3 , NM_001275757.1
    Accession Version NM_176509.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product tincar, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176509.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788686.1
    CDS826..5367
    Translation

    Target ORF information:

    RefSeq Version NM_176509.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster tincar (tinc), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176509.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05276
    Clone ID Related Accession (Same CDS sequence) NM_176509.4 , NM_176510.5 , NM_176511.3 , NM_001275757.1
    Accession Version NM_176510.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product tincar, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176510.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788687.3
    CDS736..5277
    Translation

    Target ORF information:

    RefSeq Version NM_176510.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster tincar (tinc), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176510.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05276
    Clone ID Related Accession (Same CDS sequence) NM_176509.4 , NM_176510.5 , NM_176511.3 , NM_001275757.1
    Accession Version NM_176511.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product tincar, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176511.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788688.2
    CDS784..5325
    Translation

    Target ORF information:

    RefSeq Version NM_176511.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster tincar (tinc), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176511.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05276
    Clone ID Related Accession (Same CDS sequence) NM_176509.4 , NM_176510.5 , NM_176511.3 , NM_001275757.1
    Accession Version NM_001275757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product tincar, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262686.1
    CDS739..5280
    Translation

    Target ORF information:

    RefSeq Version NM_001275757.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster tincar (tinc), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGTGGCA AGCACCAGGG ATCGGGAGCA GGAGCCTCCT CCGGAGGCGG CAGCCTCAAC 
    TCCAGCCTCT GCAATTCCGT AATGACGAAC GCCACAACGG CGAGCAGCAG CCTCACGCAA
    CAGCAGCAGC AGCTCCAGGC CAAGTACATC AAATCGAAGC GCCACCAGAG CCGCTACACC
    AGCCTCCAGC ACTCCGGCCA CGATTCCGGC AGCTATCTGC ATCTGAACTC GCTATGGTCG
    ATCTGGTACG GAGTGATGCT CACCCTGTTC CAGGGATACT TGGCCATGCA CGGAGCGTAC
    AGGTTCTTGG GATGCTCTTT GATACCCTGG AAAATCGAGC CTGTGGCCGA ACTCAATCTG
    CAGATTGTCC TTTCTGGAGT GGTGTTCATC CTGCTGCCGG TCTTTTTTAC CTCTGCCGTA
    TTTAAGGTCG GCAACTTGGC AAACGACGGA ATTAAATTGG CCACAGGTGC CAGGGAGCGG
    CGCTGTACCT TATCGCCGCA CGATGGCCTG GAGGAGGAAT CACGTGGCGG CACCCTTCGA
    GCCCTTTGGA CACACGGCGG ACCCACGGCG GCCTTCGTTC ACATCGTGAT TGCACTCTGC
    CTGCTGCTGC CGCGTTTGCT GCTCGAGGCT CGCATCATTG AGAACGGATT GCTGCCAAAA
    GAGCAAATTT GGGCCACTGA ATTGGACTTT GTTGTGATCA ATCGACGCAA TTTGATGGCC
    ATGTCGGTGG TGGGAGCCAC ACCCTTTCCC AGGCACCATC AGCCATCGAA CCAGCAGCAG
    TCCCACCACC AGCATGGCAG ACTAAACTTG ACCGCAAACA GCTTGGAACA GGACGAGGAG
    GACTACTTCA ACGATACCAT GTTCACGGCA ATCCGTGGAG TGCCAGCCGG GGGCAATAAC
    AATTTGTTCG ACCTGCGACC CGATAGGATG GCTAACGGCA GGAAGGCGGC CAAGACGACG
    GCGACGACCA GGACAACAAC CACCGCCAAC AAACTGGATG CGGGCAAGGG GCAATACTTT
    GAAGTGCCCG ATTTAATTAA TCAAGATATC GACGAGGAGG AGCGCCAAGA ACTGGAGGAG
    GCGATTCGGG GCGAGGAAGA TGATGGAGAT GAAGGTGTGG GTGTCGGCGA AGAGGGAACC
    GTCATCATGC CAGATTTCGA TGAGTTGGCG CCAAGAACCG CAGTTGGAAC GTCAACCACA
    ACGGCTAAGG CGTCCTCTGA TGCCGACAAG TTAAATGTGG AAAATTGGCA ACAGCTGGGA
    ACTCTGGGCA AAGCGGACGC ATCGAGCAGC TCCAGTAGCA CAACATCTAC CACAACAACA
    ACTACGACAT CGACAACAAC CACTGCAGCC ACAACAACTA GTACAAGGGG CACCAGCACC
    ACCACAACCA CCACCACTAT TAAACCCATG GAGATAACAA CCAGCAGACA ATCAGCACAT
    CACCATCATG GAAAGTCCCG CAAACACCAC AAACATCACA ATAAACAGCG TCAACAGCAG
    CCACCGCGTC GCCATCATGT GGCCTCCCAC GAGCAGGCGA TCCTCGAGAG CTTCCCAGAG
    GAGGAGACCA CCACCCGCGA CAGCAGCATC CATCGGGTAA GGCCCGAAGT CCTGCCGGAG
    CTACCGATTC CCTCGACGCC CGTCTCAGCC TCCAACTCCA AGATCATTGC CATCAAAGCG
    AAGAACCAAA AGCGCAGGAT ATCCAAACGG GCTGCCGGAG TTGAAATCGA GCCGGAATAT
    CTGCTTGGCG ATGCCAATAT AAATTCCAGT GAATTCGTGG CCAAATCGAA TGAGGAGGAG
    CCAGAGGAGA GCGAGGACTT CGAACTGGAG GATGGTGATT TTCAGGCAGT GCCCGCCTTG
    ACTCCTGCAC CTCCTGCTGA ACCAAACGGC CCGAAGTCAG GGCAGGATTA CGTCCGACTG
    GATGGCTTTG CCGGAATGCT GCAGCTCTTC TTTGGCATTG ATAAACCCAT CGATGTGGCA
    ATTTTCTCCC AGCCACCCAG TGCCGAGTTT GTGAACCTAC TTTGCGCCCT GTTGGTTTGG
    TCCGTTCGGT ATCCGGCCGT TTTCTGGAAT ACCTCAAAGG CCTTTGCCTG CGTTTTCAGC
    CTCCAGATGG TGGTGGCCGC CCTGGACATC ATACTTGGTT ATGTGGGCAT CTCGAATCTG
    TACAAGCTAC AGATCTACGC CGAGGCGATG CCGGTTCATC AGCCGGGACT CATCCTGAAT
    GCAGTGGTCA CTTTGGCTTT GTATCTTTTG TCCACCGCTT TGGTGTTGGC CTCTTCGATG
    GTCATGTACT TGTACGGACA CGGACGTCTG GCCACCCGGA TGCGGGATCG CTCCATTATC
    ACGTTGAAGA CTCACCAAAC CTGGATTTAC TTCGCCCACT GCGCCTCCTT GTGCTTCGTC
    CTCGCCTTGG CGGTGGTGAA GGCTCCGCTA CTCAACGATC TCAGTGCCAC CTACAAGAAC
    AACCTGCACT GCCCCACCTT CCTAGCAGCT CTTGTCGGTG TGACACACCT GCTGCTATGG
    ATTGTCATCT GGCTCTGCCT GACCATCAAG CGGCGCTGGC ACTTTAAGCT GCCTCCATTG
    GACAGCACTT ACGGTGGACT GCTGGGAAAG TCCAGTGCCC AGCCGCTCCT GATGTCCAGT
    GGCCAGCGTA CGGGGAGCAA CTCGAGCAGC AGTGGCTGCA ACTCGACCAG CACCACGGTG
    AATGGTGGTG ACTCCAAGCC GGACATGATG AGCACAGCCA CCAGCACGGA ACTGGGAATG
    GGAATGGGCA TGGGCATAGG TGGGGGCATC AATGGGGGCA TGGGCGGGAC GGGCATGGGA
    CTGGCCGCCC AGGAGGACAT CTATTGGCCC AAGCTGACGC CCAGTTCGCC CAAGCTGAAG
    GTCACCTTCA ACGAAGTTAC GAGTACGTCT GATGATGTCC TACTAATTGG TGACCAAGAA
    CAAACTGATG GCAAGCGCCA TACGAGCCGT GGAGCCTCGG TATGTTTTGC CAGTGCTACG
    GGGGAAGTTG ACGACGGCGA GTACGCGACA CTAAGGGCAG CCACCGCCGG CGCCGTCGTG
    GGCATCACCA TGGGCAGCAT GAAAAGGGGC CTCAGCAACA CCTCGGTGGG GGTAAGTCTG
    CTCCACCTTT CCGAGTACGA CGAACTTCCT CCGCCGCCGC CGGCCAACCT TCACCATCAG
    CAGCAGCAGC AGCAGCAACA GCAGCAGCGG CAGGCCCACT CCTTTGCCCA TGGACATCCC
    CACGACTATG CGAATCTGAG CGGATTGGGT GGCATCAGTG ACGACAACAT CTCCGAGGAG
    GGCAAGCTGC TGGCCTGTGT GCGCGATGAC AGTATCACCT ACGCATCCAC CAGTGATTTG
    GAGCCACCAC AGCCATCGGC CCAGGCACCA CCACCACCAC CACCGCTGCC CATAAAGGGA
    GCACCCGTTC CTCAGCCACC AGCGGTAATG CCCCACTCTG CCGGCATCTA CGGACGCGCT
    CCTCAAGCAA TGCCCGAGAT GATGCAGTTG TCGCCCGAGC ACCACCACAA TCCACTTCAG
    CACTCGCTGC AGCATCCACA GCACCATCCT CTTCAGCAGC AGCAGCAGAA GCAGCAGACA
    CCACAGCATC CTCTCCAGCA GCAGGGCAAT CCGCACCAGC ATCTAGTCAG CCCCTTGGCT
    CCGGTCACGG TCGCAGTTCA CACCAACGAA GCGCATATTG CATCCAGTTC CACACCCCGT
    TGCCTGCGTC GCGCCGATTC GGGCGTTCCC AACGAGGCGT TGACACCGCG CAGCGACACC
    ACCTCCACCA CCGAAAGTAC CAACACCACC TCACCGCCGG AACGCGCCCC CTCCGAGTCC
    TCCAGTGGCG TCCACTCGGG CGAGGAACGT GAGCTGGAGG TCATCATCCG GCCAAGGGCC
    AACAGCAAGC CGCCGCCACG CCCCCCACAG CCGCCGATCC AGGAGGAGCC ATACGGCCGC
    TGCACCAACA TGCGCATGTC CAGCTTCAAT GCGGATCCCG CCGCCACCTC GTCGGCGGTC
    ATCAATAGTG CCACATTGCC GCCGCAGCGT TCTGTGCCGG AGCAGAAGTT CGACTATACG
    GCTCACTGCA GCACAATGCC ACTGCCGGTG GGCTGTCACA GCCAGCAACT CGCCGGGAGC
    GGTAATGGCT ATGCCTCCAC CTCGGCAATG ACCAGTTCCA TGGGCGGTGG CGGTATGCCA
    CCACTGCCGC CATCGCAGGT CTCCAGCTTC ATGACGCCCA ACAGTATGCA CTACGCCAAT
    GCATCAGTGG CCCTGGGAAA TGTTAGTGGT CAACAGCAGC CGCACACCAC GCTGCCAAAT
    GGAGTGCGCT ACTCCAATCC GCACTTTCTG CGCCGCCTGC CGCACGTGAC CAAGGCGGCG
    GAGTCGCCGT ACGGACACCT GGGCTACGGA GCTGGTCACC ACGCCTTCGC AAAGCTGCCG
    CACGAGACGC ATCCCACGAT TCCGGAGGAT CGCGACTCCG CCAACTACTC GATGGCCTCT
    GACCAGGATT GCGGACTCTA TGTGACGGCC CAGCTGCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.