ss cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ss
Entrez Gene ID	41988
Full Name	spineless
Synonyms	AHR,AhR,CG6993,Dmel\CG6993,SS,Ss,ssa
General protein information	Preferred Names spineless Names CG6993 gene product from transcript CG6993-RA CG6993-PA CG6993-PC CG6993-PD aristapedia spineless-aristapedia ss-PA ss-PC ss-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B14-89B15\|3-58.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170158.3	NP_001163629.2	spineless, isoform D
NM_001260211.2	NP_001247140.1	spineless, isoform C
NM_057400.4	NP_476748.1	spineless, isoform A

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ahr-1	NP_001021036.1
Drosophila melanogaster (fruit fly)	ss	NP_001247140.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

although individual ss alleles make independent stochastic choices, interchromosomal communication coordinates expression state between alleles, ensuring that they are both expressed in the same random subset of R7 photoreceptors.
Title: Interchromosomal communication coordinates intrinsically stochastic expression between alleles.

The function of Antennapedia in the leg is not to repress homothorax, as has been suggested, but to directly repress spineless and other antennal genes that would otherwise be activated within this ring.
Title: Control of the spineless antennal enhancer: direct repression of antennal target genes by Antennapedia.

Studies suggest there are two apparently different functions of Ahr, dioxin-mediated and dioxin-independent, are in fact two different levels (hyperactivated and basal, respectively) of a single function.
Title: Dioxin toxicity in vivo results from an increase in the dioxin-independent transcriptional activity of the aryl hydrocarbon receptor.

Interaction of the ss and CG5017 genes in the regulation of morphogensis of limbs in Drosophila melanogaster
Title: [Interaction of the ss and CG5017 genes in the regulation of morphogensis of limbs in Drosophila melanogaster].

Six chimeric proteins clarified that amino acids 291-350 of the ligand-binding domain, i.e. the middle region, were required to keep the protein in the active form in the absence of ligand binding.
Title: Characterization of the region of the aryl hydrocarbon receptor required for ligand dependency of transactivation using chimeric receptor between Drosophila and Mus musculus.

The following ss gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ss cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05117	NM_057400.4 Latest version!	Drosophila melanogaster spineless (ss), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07928	NM_001260211.2 Latest version!	Drosophila melanogaster spineless (ss), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa07928	NM_001170158.3 Latest version!	Drosophila melanogaster spineless (ss), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ss ( NM_057400.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476748.1 Drosophila melanogaster spineless (ss), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05117
Clone ID Related Accession (Same CDS sequence)	NM_057400.4
Accession Version	NM_057400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spineless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_057400.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCAGC TGGGCACCGT CTACGCCACC AAGCGAAGGC GACGCAACGG CAAGAGCCTG 
AAGCCGCCGC CCAAGGATGG CGTCACAAAG AGCAATCCAT CAAAGCGGCA TCGGGAGCGC 
CTCAACGCCG AGCTGGATCT GCTGGCCTCG CTGCTCCCCT TCGAGCAGAA CATCTTGAGC 
AAACTGGATC GACTGAGCAT TCTAAGGCTG TCTGTTAGTT ATTTAAGAAC CAAAAGTTAT 
TTTCAAGTTG TTATGCATAA GGATAAGGAG GATAACGGAG TCCTGCCCCA CATACACGCA 
CATGACGGCT ACAGGACACG AGAACTAGGC GCCTTCGAGC ACGGCCTGTT GGATGGTGAT 
ATGTTCCTCC AGGCCCTAAA TGGATTTCTA ATGATACTGA CATGCGAAGG CGAAGTCTTC 
TTTGCCACGC ACAGCATCGA GAGCTATTTG GGTTTTCATC AGTCGGACAT CGTCCACCAG 
TCGGTGTACG AACTGGTGCA CTCGGAGGAC CGCGAGGAGC TGCAGCGCCA GCTGCTGTGG 
AACAGTTTCC TGCCCGCCGA CATGTCCAGC ATGCAGCTGG CGGAGACCCT GGCGCCGGAC 
AAGGCGCTCT ACCTGGAGCG CAGCTTCACC GTCCGCTTCC GCTGCCTGCT GGACAACACG 
AGCGGCTTCC TGCGCCTGGA CATCCGTGGC CGCATCAAGG TCCTGCATGG CCAGAACCGC 
AAGACGGAGG AGCCACCGCT GGCCCTCTTT GCCTACTGCA CGCCCTTCGG GCCGCCCAGC 
CTGCTGGAAA TCCCGCACAA GGAGAACATG TTCAAGTCCA AGCACAAGCT GGACTTCTCC 
CTGGTATCAA TGGACCAGCG CGGCAAGCAC ATCCTGGGCT ACGCGGACGC CGAGCTGGTC 
AACATGGGCG GCTACGATCT GGTGCACTAC GATGACCTTG CCTATGTGGC CAGCGCCCAT 
CAGGAGCTTT TGAAGACGGG TGCCTCTGGC ATGATCGCCT ACCGTTACCA AAAGAAGGAT 
GGCGAGTGGC AATGGCTGCA GACGAGCTCG CGCCTGGTCT ACAAGAACTC CAAGCCGGAC 
TTTGTGATCT GTACGCACCG CCAGCTGATG GACGAGGAGG GCCACGATCT GCTGGGCAAG 
CGCACCATGG ACTTTAAGGT CAGCTACCTG GACACGGGAC TGGCGTCCAC CTACTTCTCC 
GAGGCGGACC AGCTGGTAGT GCCTCCGAGC ACCTCCCCCA CGGCCCACGC TCTGCCGCCG 
CCGGTGACGC CAACGCGTCC AAATCGCCGC TACAAGACGC AGTTGCGCGA CTTCCTTTCC 
ACTTGTCGCA GCAAACGCAA GCTCCAGCAG CAGAACCAAC CACAGACGCA GCAAACCTCA 
CCACTTGGTG GCCAGGTGGG ATCCCCTGCT CCGGCTGTAG CCGTGGAGTA CTTGCCCGAT 
CCGGCAGCTG CAGTGGCCGC TGCCTACTCC AACCTAAATC CCATGTACAC AACCTCGCCG 
TATGCCAGTG CCGCGGACAA TCTATACATG GGTAGCTCCA TGCCGGCCAA CGCCTTCTAC 
CCAGTCAGCG AGAACCTCTT CCATCAGTAC CGGCTGCAGG GCGCCGTCGG TGGCTACTAC 
ACGGATTATC CCCACTCCGG CGCTCCAGCC TCAGCTTACG TAGCTAATGG ATTTCTCTCT 
TACGACGGAT ACGCCATTGC TTCCAAAGCG GACGAAAAGT GGCAAGAGAC TGGAAAGTAC 
TACAGTGGCT ACAGCAGTGG CTATGGAAGT CCAACATCTA CGCCACAGGT ACGAAAACAA 
ATTCCTCTGA AGACGCCGAA ATCTTCACCC CAGGTGATGG AGGTGATATC TTGCTCCTCG 
GACGGTCCAT CACCTGTGGG CGGAGCCACG CCCAACGGAG TCGGAAGCGT TACCCCCAAA 
GTTGAGTTGG CCGGAACAAC GGCGGCAGCA GCAGCGGGTC AAGATCCCTA CGAGCGTCAA 
ACGGTGCTGA TGTGGGGCAC CACGCACTCG AGTGGGGTAC CGCTAAATAG TCATGGCGGT 
CGAACTGGAG CCGGAAACCC TGCCTCACCA CAGCGATCCA CTCCTCTTGG CAACGGATTG 
GGCTACGCAA GCGCCAATAA TAACAACAAC GATCTTGAAC CTGCCACGGC TGCCAAGTGG 
AACGGAACAA AGGAGCTGCC GGGCAAGTCG GGCAGTGCCA GTACGCCGGA GAGCTACCAG 
ATGCAGCATG ATGATTCCGG CCTTTACTCC GCCTCTTCGC ACACAACCTC TCCGCAGCAG 
CAACAACAGC AGCAGCTCCA ACAATCACAG CGAGGAGTTG GTTCCAATGT TAGCGCTCCC 
AGCAGCTCAC TCACTAGCAC GGGCACAGAT CAGCAGGCGG TGCACCCATC CAGTTGCCAT 
CAACAACAGC AGCAGCAGCA GCAACAACAC CATCACGCCC ACCCGCATCC GCACTCGCAT 
CATCACCACC ATCATCACCA TCACCATGAG ACGGCGCATC AGCACAGCAG CGAGGTATGG 
ACGCCCGCCT CCTACACGCA GTACTCGCAG TACTTCACGT ACCATCATCC GCATCCGCAC 
CCACACCATC CGTCGGCTGG CGGCGGTGGC CATGTTCCGG CCCAGTCGCA TCACCTGCAC 
CACGGCCACC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476748.1
CDS	738..3392
Translation	MSQLGTVYATKRRRRNGKSLKPPPKDGVTKSNPSKRHRERLNAELDLLASLLPFEQNILSKLDRLSILRLSVSYLRTKSYFQVVMHKDKEDNGVLPHIHAHDGYRTRELGAFEHGLLDGDMFLQALNGFLMILTCEGEVFFATHSIESYLGFHQSDIVHQSVYELVHSEDREELQRQLLWNSFLPADMSSMQLAETLAPDKALYLERSFTVRFRCLLDNTSGFLRLDIRGRIKVLHGQNRKTEEPPLALFAYCTPFGPPSLLEIPHKENMFKSKHKLDFSLVSMDQRGKHILGYADAELVNMGGYDLVHYDDLAYVASAHQELLKTGASGMIAYRYQKKDGEWQWLQTSSRLVYKNSKPDFVICTHRQLMDEEGHDLLGKRTMDFKVSYLDTGLASTYFSEADQLVVPPSTSPTAHALPPPVTPTRPNRRYKTQLRDFLSTCRSKRKLQQQNQPQTQQTSPLGGQVGSPAPAVAVEYLPDPAAAVAAAYSNLNPMYTTSPYASAADNLYMGSSMPANAFYPVSENLFHQYRLQGAVGGYYTDYPHSGAPASAYVANGFLSYDGYAIASKADEKWQETGKYYSGYSSGYGSPTSTPQVRKQIPLKTPKSSPQVMEVISCSSDGPSPVGGATPNGVGSVTPKVELAGTTAAAAAGQDPYERQTVLMWGTTHSSGVPLNSHGGRTGAGNPASPQRSTPLGNGLGYASANNNNNDLEPATAAKWNGTKELPGKSGSASTPESYQMQHDDSGLYSASSHTTSPQQQQQQQLQQSQRGVGSNVSAPSSSLTSTGTDQQAVHPSSCHQQQQQQQQQHHHAHPHPHSHHHHHHHHHHETAHQHSSEVWTPASYTQYSQYFTYHHPHPHPHHPSAGGGGHVPAQSHHLHHGHR

Target ORF information:

RefSeq Version	NM_057400.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spineless (ss), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057400.4

ORF Insert Sequence:

ATGAGCCAGC TGGGCACCGT CTACGCCACC AAGCGAAGGC GACGCAACGG CAAGAGCCTG 
AAGCCGCCGC CCAAGGATGG CGTCACAAAG AGCAATCCAT CAAAGCGGCA TCGGGAGCGC 
CTCAACGCCG AGCTGGATCT GCTGGCCTCG CTGCTCCCCT TCGAGCAGAA CATCTTGAGC 
AAACTGGATC GACTGAGCAT TCTAAGGCTG TCTGTTAGTT ATTTAAGAAC CAAAAGTTAT 
TTTCAAGTTG TTATGCATAA GGATAAGGAG GATAACGGAG TCCTGCCCCA CATACACGCA 
CATGACGGCT ACAGGACACG AGAACTAGGC GCCTTCGAGC ACGGCCTGTT GGATGGTGAT 
ATGTTCCTCC AGGCCCTAAA TGGATTTCTA ATGATACTGA CATGCGAAGG CGAAGTCTTC 
TTTGCCACGC ACAGCATCGA GAGCTATTTG GGTTTTCATC AGTCGGACAT CGTCCACCAG 
TCGGTGTACG AACTGGTGCA CTCGGAGGAC CGCGAGGAGC TGCAGCGCCA GCTGCTGTGG 
AACAGTTTCC TGCCCGCCGA CATGTCCAGC ATGCAGCTGG CGGAGACCCT GGCGCCGGAC 
AAGGCGCTCT ACCTGGAGCG CAGCTTCACC GTCCGCTTCC GCTGCCTGCT GGACAACACG 
AGCGGCTTCC TGCGCCTGGA CATCCGTGGC CGCATCAAGG TCCTGCATGG CCAGAACCGC 
AAGACGGAGG AGCCACCGCT GGCCCTCTTT GCCTACTGCA CGCCCTTCGG GCCGCCCAGC 
CTGCTGGAAA TCCCGCACAA GGAGAACATG TTCAAGTCCA AGCACAAGCT GGACTTCTCC 
CTGGTATCAA TGGACCAGCG CGGCAAGCAC ATCCTGGGCT ACGCGGACGC CGAGCTGGTC 
AACATGGGCG GCTACGATCT GGTGCACTAC GATGACCTTG CCTATGTGGC CAGCGCCCAT 
CAGGAGCTTT TGAAGACGGG TGCCTCTGGC ATGATCGCCT ACCGTTACCA AAAGAAGGAT 
GGCGAGTGGC AATGGCTGCA GACGAGCTCG CGCCTGGTCT ACAAGAACTC CAAGCCGGAC 
TTTGTGATCT GTACGCACCG CCAGCTGATG GACGAGGAGG GCCACGATCT GCTGGGCAAG 
CGCACCATGG ACTTTAAGGT CAGCTACCTG GACACGGGAC TGGCGTCCAC CTACTTCTCC 
GAGGCGGACC AGCTGGTAGT GCCTCCGAGC ACCTCCCCCA CGGCCCACGC TCTGCCGCCG 
CCGGTGACGC CAACGCGTCC AAATCGCCGC TACAAGACGC AGTTGCGCGA CTTCCTTTCC 
ACTTGTCGCA GCAAACGCAA GCTCCAGCAG CAGAACCAAC CACAGACGCA GCAAACCTCA 
CCACTTGGTG GCCAGGTGGG ATCCCCTGCT CCGGCTGTAG CCGTGGAGTA CTTGCCCGAT 
CCGGCAGCTG CAGTGGCCGC TGCCTACTCC AACCTAAATC CCATGTACAC AACCTCGCCG 
TATGCCAGTG CCGCGGACAA TCTATACATG GGTAGCTCCA TGCCGGCCAA CGCCTTCTAC 
CCAGTCAGCG AGAACCTCTT CCATCAGTAC CGGCTGCAGG GCGCCGTCGG TGGCTACTAC 
ACGGATTATC CCCACTCCGG CGCTCCAGCC TCAGCTTACG TAGCTAATGG ATTTCTCTCT 
TACGACGGAT ACGCCATTGC TTCCAAAGCG GACGAAAAGT GGCAAGAGAC TGGAAAGTAC 
TACAGTGGCT ACAGCAGTGG CTATGGAAGT CCAACATCTA CGCCACAGGT ACGAAAACAA 
ATTCCTCTGA AGACGCCGAA ATCTTCACCC CAGGTGATGG AGGTGATATC TTGCTCCTCG 
GACGGTCCAT CACCTGTGGG CGGAGCCACG CCCAACGGAG TCGGAAGCGT TACCCCCAAA 
GTTGAGTTGG CCGGAACAAC GGCGGCAGCA GCAGCGGGTC AAGATCCCTA CGAGCGTCAA 
ACGGTGCTGA TGTGGGGCAC CACGCACTCG AGTGGGGTAC CGCTAAATAG TCATGGCGGT 
CGAACTGGAG CCGGAAACCC TGCCTCACCA CAGCGATCCA CTCCTCTTGG CAACGGATTG 
GGCTACGCAA GCGCCAATAA TAACAACAAC GATCTTGAAC CTGCCACGGC TGCCAAGTGG 
AACGGAACAA AGGAGCTGCC GGGCAAGTCG GGCAGTGCCA GTACGCCGGA GAGCTACCAG 
ATGCAGCATG ATGATTCCGG CCTTTACTCC GCCTCTTCGC ACACAACCTC TCCGCAGCAG 
CAACAACAGC AGCAGCTCCA ACAATCACAG CGAGGAGTTG GTTCCAATGT TAGCGCTCCC 
AGCAGCTCAC TCACTAGCAC GGGCACAGAT CAGCAGGCGG TGCACCCATC CAGTTGCCAT 
CAACAACAGC AGCAGCAGCA GCAACAACAC CATCACGCCC ACCCGCATCC GCACTCGCAT 
CATCACCACC ATCATCACCA TCACCATGAG ACGGCGCATC AGCACAGCAG CGAGGTATGG 
ACGCCCGCCT CCTACACGCA GTACTCGCAG TACTTCACGT ACCATCATCC GCATCCGCAC 
CCACACCATC CGTCGGCTGG CGGCGGTGGC CATGTTCCGG CCCAGTCGCA TCACCTGCAC 
CACGGCCACC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ss ( NM_001170158.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163629.2 Drosophila melanogaster spineless (ss), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa07928
Clone ID Related Accession (Same CDS sequence)	NM_001170158.3 , NM_001260211.2
Accession Version	NM_001170158.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spineless, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170158.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCAGC TGGGCACCGT CTACGCCACC AAGCGAAGGC GACGCAACGG CAAGAGCCTG 
AAGCCGCCGC CCAAGGATGG CGTCACAAAG AGCAATCCAT CAAAGCGGCA TCGGGAGCGC 
CTCAACGCCG AGCTGGATCT GCTGGCCTCG CTGCTCCCCT TCGAGCAGAA CATCTTGAGC 
AAACTGGATC GACTGAGCAT TCTAAGGCTG TCTGTTAGTT ATTTAAGAAC CAAAAGTTAT 
TTTCAAGTTG TTATGCATAA GGATAAGGAG GATAACGGAG TCCTGCCCCA CATACACGCA 
CATGACGGCT ACAGGACACG AGAACTAGGC GCCTTCGAGC ACGGCCTGTT GGATGGTGAT 
ATGTTCCTCC AGGCCCTAAA TGGATTTCTA ATGATACTGA CATGCGAAGG CGAAGTCTTC 
TTTGCCACGC ACAGCATCGA GAGCTATTTG GGTTTTCATC AGTCGGACAT CGTCCACCAG 
TCGGTGTACG AACTGGTGCA CTCGGAGGAC CGCGAGGAGC TGCAGCGCCA GCTGCTGTGG 
AACAGTTTCC TGCCCGCCGA CATGTCCAGC ATGCAGCTGG CGGAGACCCT GGCGCCGGAC 
AAGGCGCTCT ACCTGGAGCG CAGCTTCACC GTCCGCTTCC GCTGCCTGCT GGACAACACG 
AGCGGCTTCC TGCGCCTGGA CATCCGTGGC CGCATCAAGG TCCTGCATGG CCAGAACCGC 
AAGACGGAGG AGCCACCGCT GGCCCTCTTT GCCTACTGCA CGCCCTTCGG GCCGCCCAGC 
CTGCTGGAAA TCCCGCACAA GGAGAACATG TTCAAGTCCA AGCACAAGCT GGACTTCTCC 
CTGGTATCAA TGGACCAGCG CGGCAAGCAC ATCCTGGGCT ACGCGGACGC CGAGCTGGTC 
AACATGGGCG GCTACGATCT GGTGCACTAC GATGACCTTG CCTATGTGGC CAGCGCCCAT 
CAGGAGCTTT TGAAGACGGG TGCCTCTGGC ATGATCGCCT ACCGTTACCA AAAGAAGGAT 
GGCGAGTGGC AATGGCTGCA GACGAGCTCG CGCCTGGTCT ACAAGAACTC CAAGCCGGAC 
TTTGTGATCT GTACGCACCG CCAGCTGATG GACGAGGAGG GCCACGATCT GCTGGGCAAG 
CGCACCATGG ACTTTAAGGT CAGCTACCTG GACACGGGAC TGGCGTCCAC CTACTTCTCC 
GAGGCGGACC AGCTGGTAGT GCCTCCGAGC ACCTCCCCCA CGGCCCACGC TCTGCCGCCG 
CCGGTGACGC CAACGCGTCC AAATCGCCGC TACAAGACGC AGTTGCGCGA CTTCCTTTCC 
ACTTGTCGCA GCAAACGCAA GCTCCAGCAG CAGAACCAAC CACAGACGCA GCAAACCTCA 
CCACTTGGTG GCCAGGTGGG ATCCCCTGCT CCGGCTGTAG CCGTGGAGTA CTTGCCCGAT 
CCGGCAGCTG CAGTGGCCGC TGCCTACTCC AACCTAAATC CCATGTACAC AACCTCGCCG 
TATGCCAGTG CCGCGGACAA TCTATACATG GGTAGCTCCA TGCCGGCCAA CGCCTTCTAC 
CCAGTCAGCG AGAACCTCTT CCATCAGTAC CGGCTGCAGG GCGCCGTCGG TGGCTACTAC 
ACGGATTATC CCCACTCCGG CGCTCCAGCC TCAGCTTACG TAGCTAATGG ATTTCTCTCT 
TACGACGGAT ACGCCATTGC TTCCAAAGCG GACGAAAAGT GGCAAGAGAC TGGAAAGTAC 
TACAGTGGCT ACAGCAGTGG CTATGGAAGT CCAACATCTA CGCCACAGCA AATTCCTCTG 
AAGACGCCGA AATCTTCACC CCAGGTGATG GAGGTGATAT CTTGCTCCTC GGACGGTCCA 
TCACCTGTGG GCGGAGCCAC GCCCAACGGA GTCGGAAGCG TTACCCCCAA AGTTGAGTTG 
GCCGGAACAA CGGCGGCAGC AGCAGCGGGT CAAGATCCCT ACGAGCGTCA AACGGTGCTG 
ATGTGGGGCA CCACGCACTC GAGTGGGGTA CCGCTAAATA GTCATGGCGG TCGAACTGGA 
GCCGGAAACC CTGCCTCACC ACAGCGATCC ACTCCTCTTG GCAACGGATT GGGCTACGCA 
AGCGCCAATA ATAACAACAA CGATCTTGAA CCTGCCACGG CTGCCAAGTG GAACGGAACA 
AAGGAGCTGC CGGGCAAGTC GGGCAGTGCC AGTACGCCGG AGAGCTACCA GATGCAGCAT 
GATGATTCCG GCCTTTACTC CGCCTCTTCG CACACAACCT CTCCGCAGCA GCAACAACAG 
CAGCAGCTCC AACAATCACA GCGAGGAGTT GGTTCCAATG TTAGCGCTCC CAGCAGCTCA 
CTCACTAGCA CGGGCACAGA TCAGCAGGCG GTGCACCCAT CCAGTTGCCA TCAACAACAG 
CAGCAGCAGC AGCAACAACA CCATCACGCC CACCCGCATC CGCACTCGCA TCATCACCAC 
CATCATCACC ATCACCATGA GACGGCGCAT CAGCACAGCA GCGAGATCAT TCTCACCACC 
CATCATCAGC AACAGTCGCA GCAGCAGCAG CAATCGCTCG CTGGCTACCC GCTGCACCAC 
CAGCTGGTGG GAGTTGGATC AGGATCACCG CCGCCGCAGC CGCCAGTCGC ATTGTCCCGC 
TCGCCCACGA CCTTGCCGGC GGTCAGCAGC TTGCTGAATG GATCGGCTTC AGCAGGAGGA 
GCATCGGATA TTCCATACCA GCAATTGGCC ATTGTGTCCG CTCCTCTGGT GATTTGCGCC 
GAGGAGGTGG GTGGAGGCGT CAGCAGCATT GGCCGCAAGT CATCCAAGCA GCAACAGTCC 
CTGCAACACC TGCAGCAGCA ACAGTCACTG CAACAGCAGC AACATGCAAT TTATCAGCAG 
CAACATCACC ACTCGCAGCA TCATCCGCAT CATCAGCTGC TCTATCCGGC GAACATTACG 
GCGCACACCG CGCTGGCTTA TGCCCCGCCC ACGGCCGGAG GCGCATATGC CGATGGCAGT 
CCGCTGCTCT CCTTCTCCGA GGTGACCAAC ACGCTACTGA ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163629.2
CDS	701..3748
Translation	MSQLGTVYATKRRRRNGKSLKPPPKDGVTKSNPSKRHRERLNAELDLLASLLPFEQNILSKLDRLSILRLSVSYLRTKSYFQVVMHKDKEDNGVLPHIHAHDGYRTRELGAFEHGLLDGDMFLQALNGFLMILTCEGEVFFATHSIESYLGFHQSDIVHQSVYELVHSEDREELQRQLLWNSFLPADMSSMQLAETLAPDKALYLERSFTVRFRCLLDNTSGFLRLDIRGRIKVLHGQNRKTEEPPLALFAYCTPFGPPSLLEIPHKENMFKSKHKLDFSLVSMDQRGKHILGYADAELVNMGGYDLVHYDDLAYVASAHQELLKTGASGMIAYRYQKKDGEWQWLQTSSRLVYKNSKPDFVICTHRQLMDEEGHDLLGKRTMDFKVSYLDTGLASTYFSEADQLVVPPSTSPTAHALPPPVTPTRPNRRYKTQLRDFLSTCRSKRKLQQQNQPQTQQTSPLGGQVGSPAPAVAVEYLPDPAAAVAAAYSNLNPMYTTSPYASAADNLYMGSSMPANAFYPVSENLFHQYRLQGAVGGYYTDYPHSGAPASAYVANGFLSYDGYAIASKADEKWQETGKYYSGYSSGYGSPTSTPQQIPLKTPKSSPQVMEVISCSSDGPSPVGGATPNGVGSVTPKVELAGTTAAAAAGQDPYERQTVLMWGTTHSSGVPLNSHGGRTGAGNPASPQRSTPLGNGLGYASANNNNNDLEPATAAKWNGTKELPGKSGSASTPESYQMQHDDSGLYSASSHTTSPQQQQQQQLQQSQRGVGSNVSAPSSSLTSTGTDQQAVHPSSCHQQQQQQQQQHHHAHPHPHSHHHHHHHHHHETAHQHSSEIILTTHHQQQSQQQQQSLAGYPLHHQLVGVGSGSPPPQPPVALSRSPTTLPAVSSLLNGSASAGGASDIPYQQLAIVSAPLVICAEEVGGGVSSIGRKSSKQQQSLQHLQQQQSLQQQQHAIYQQQHHHSQHHPHHQLLYPANITAHTALAYAPPTAGGAYADGSPLLSFSEVTNTLLNQ

Target ORF information:

RefSeq Version	NM_001170158.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spineless (ss), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170158.3

ORF Insert Sequence:

ATGAGCCAGC TGGGCACCGT CTACGCCACC AAGCGAAGGC GACGCAACGG CAAGAGCCTG 
AAGCCGCCGC CCAAGGATGG CGTCACAAAG AGCAATCCAT CAAAGCGGCA TCGGGAGCGC 
CTCAACGCCG AGCTGGATCT GCTGGCCTCG CTGCTCCCCT TCGAGCAGAA CATCTTGAGC 
AAACTGGATC GACTGAGCAT TCTAAGGCTG TCTGTTAGTT ATTTAAGAAC CAAAAGTTAT 
TTTCAAGTTG TTATGCATAA GGATAAGGAG GATAACGGAG TCCTGCCCCA CATACACGCA 
CATGACGGCT ACAGGACACG AGAACTAGGC GCCTTCGAGC ACGGCCTGTT GGATGGTGAT 
ATGTTCCTCC AGGCCCTAAA TGGATTTCTA ATGATACTGA CATGCGAAGG CGAAGTCTTC 
TTTGCCACGC ACAGCATCGA GAGCTATTTG GGTTTTCATC AGTCGGACAT CGTCCACCAG 
TCGGTGTACG AACTGGTGCA CTCGGAGGAC CGCGAGGAGC TGCAGCGCCA GCTGCTGTGG 
AACAGTTTCC TGCCCGCCGA CATGTCCAGC ATGCAGCTGG CGGAGACCCT GGCGCCGGAC 
AAGGCGCTCT ACCTGGAGCG CAGCTTCACC GTCCGCTTCC GCTGCCTGCT GGACAACACG 
AGCGGCTTCC TGCGCCTGGA CATCCGTGGC CGCATCAAGG TCCTGCATGG CCAGAACCGC 
AAGACGGAGG AGCCACCGCT GGCCCTCTTT GCCTACTGCA CGCCCTTCGG GCCGCCCAGC 
CTGCTGGAAA TCCCGCACAA GGAGAACATG TTCAAGTCCA AGCACAAGCT GGACTTCTCC 
CTGGTATCAA TGGACCAGCG CGGCAAGCAC ATCCTGGGCT ACGCGGACGC CGAGCTGGTC 
AACATGGGCG GCTACGATCT GGTGCACTAC GATGACCTTG CCTATGTGGC CAGCGCCCAT 
CAGGAGCTTT TGAAGACGGG TGCCTCTGGC ATGATCGCCT ACCGTTACCA AAAGAAGGAT 
GGCGAGTGGC AATGGCTGCA GACGAGCTCG CGCCTGGTCT ACAAGAACTC CAAGCCGGAC 
TTTGTGATCT GTACGCACCG CCAGCTGATG GACGAGGAGG GCCACGATCT GCTGGGCAAG 
CGCACCATGG ACTTTAAGGT CAGCTACCTG GACACGGGAC TGGCGTCCAC CTACTTCTCC 
GAGGCGGACC AGCTGGTAGT GCCTCCGAGC ACCTCCCCCA CGGCCCACGC TCTGCCGCCG 
CCGGTGACGC CAACGCGTCC AAATCGCCGC TACAAGACGC AGTTGCGCGA CTTCCTTTCC 
ACTTGTCGCA GCAAACGCAA GCTCCAGCAG CAGAACCAAC CACAGACGCA GCAAACCTCA 
CCACTTGGTG GCCAGGTGGG ATCCCCTGCT CCGGCTGTAG CCGTGGAGTA CTTGCCCGAT 
CCGGCAGCTG CAGTGGCCGC TGCCTACTCC AACCTAAATC CCATGTACAC AACCTCGCCG 
TATGCCAGTG CCGCGGACAA TCTATACATG GGTAGCTCCA TGCCGGCCAA CGCCTTCTAC 
CCAGTCAGCG AGAACCTCTT CCATCAGTAC CGGCTGCAGG GCGCCGTCGG TGGCTACTAC 
ACGGATTATC CCCACTCCGG CGCTCCAGCC TCAGCTTACG TAGCTAATGG ATTTCTCTCT 
TACGACGGAT ACGCCATTGC TTCCAAAGCG GACGAAAAGT GGCAAGAGAC TGGAAAGTAC 
TACAGTGGCT ACAGCAGTGG CTATGGAAGT CCAACATCTA CGCCACAGCA AATTCCTCTG 
AAGACGCCGA AATCTTCACC CCAGGTGATG GAGGTGATAT CTTGCTCCTC GGACGGTCCA 
TCACCTGTGG GCGGAGCCAC GCCCAACGGA GTCGGAAGCG TTACCCCCAA AGTTGAGTTG 
GCCGGAACAA CGGCGGCAGC AGCAGCGGGT CAAGATCCCT ACGAGCGTCA AACGGTGCTG 
ATGTGGGGCA CCACGCACTC GAGTGGGGTA CCGCTAAATA GTCATGGCGG TCGAACTGGA 
GCCGGAAACC CTGCCTCACC ACAGCGATCC ACTCCTCTTG GCAACGGATT GGGCTACGCA 
AGCGCCAATA ATAACAACAA CGATCTTGAA CCTGCCACGG CTGCCAAGTG GAACGGAACA 
AAGGAGCTGC CGGGCAAGTC GGGCAGTGCC AGTACGCCGG AGAGCTACCA GATGCAGCAT 
GATGATTCCG GCCTTTACTC CGCCTCTTCG CACACAACCT CTCCGCAGCA GCAACAACAG 
CAGCAGCTCC AACAATCACA GCGAGGAGTT GGTTCCAATG TTAGCGCTCC CAGCAGCTCA 
CTCACTAGCA CGGGCACAGA TCAGCAGGCG GTGCACCCAT CCAGTTGCCA TCAACAACAG 
CAGCAGCAGC AGCAACAACA CCATCACGCC CACCCGCATC CGCACTCGCA TCATCACCAC 
CATCATCACC ATCACCATGA GACGGCGCAT CAGCACAGCA GCGAGATCAT TCTCACCACC 
CATCATCAGC AACAGTCGCA GCAGCAGCAG CAATCGCTCG CTGGCTACCC GCTGCACCAC 
CAGCTGGTGG GAGTTGGATC AGGATCACCG CCGCCGCAGC CGCCAGTCGC ATTGTCCCGC 
TCGCCCACGA CCTTGCCGGC GGTCAGCAGC TTGCTGAATG GATCGGCTTC AGCAGGAGGA 
GCATCGGATA TTCCATACCA GCAATTGGCC ATTGTGTCCG CTCCTCTGGT GATTTGCGCC 
GAGGAGGTGG GTGGAGGCGT CAGCAGCATT GGCCGCAAGT CATCCAAGCA GCAACAGTCC 
CTGCAACACC TGCAGCAGCA ACAGTCACTG CAACAGCAGC AACATGCAAT TTATCAGCAG 
CAACATCACC ACTCGCAGCA TCATCCGCAT CATCAGCTGC TCTATCCGGC GAACATTACG 
GCGCACACCG CGCTGGCTTA TGCCCCGCCC ACGGCCGGAG GCGCATATGC CGATGGCAGT 
CCGCTGCTCT CCTTCTCCGA GGTGACCAAC ACGCTACTGA ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ss ( NM_001260211.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247140.1 Drosophila melanogaster spineless (ss), transcript variant C, mRNA.

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CloneID	OFa07928
Clone ID Related Accession (Same CDS sequence)	NM_001170158.3 , NM_001260211.2
Accession Version	NM_001260211.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spineless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260211.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCAGC TGGGCACCGT CTACGCCACC AAGCGAAGGC GACGCAACGG CAAGAGCCTG 
AAGCCGCCGC CCAAGGATGG CGTCACAAAG AGCAATCCAT CAAAGCGGCA TCGGGAGCGC 
CTCAACGCCG AGCTGGATCT GCTGGCCTCG CTGCTCCCCT TCGAGCAGAA CATCTTGAGC 
AAACTGGATC GACTGAGCAT TCTAAGGCTG TCTGTTAGTT ATTTAAGAAC CAAAAGTTAT 
TTTCAAGTTG TTATGCATAA GGATAAGGAG GATAACGGAG TCCTGCCCCA CATACACGCA 
CATGACGGCT ACAGGACACG AGAACTAGGC GCCTTCGAGC ACGGCCTGTT GGATGGTGAT 
ATGTTCCTCC AGGCCCTAAA TGGATTTCTA ATGATACTGA CATGCGAAGG CGAAGTCTTC 
TTTGCCACGC ACAGCATCGA GAGCTATTTG GGTTTTCATC AGTCGGACAT CGTCCACCAG 
TCGGTGTACG AACTGGTGCA CTCGGAGGAC CGCGAGGAGC TGCAGCGCCA GCTGCTGTGG 
AACAGTTTCC TGCCCGCCGA CATGTCCAGC ATGCAGCTGG CGGAGACCCT GGCGCCGGAC 
AAGGCGCTCT ACCTGGAGCG CAGCTTCACC GTCCGCTTCC GCTGCCTGCT GGACAACACG 
AGCGGCTTCC TGCGCCTGGA CATCCGTGGC CGCATCAAGG TCCTGCATGG CCAGAACCGC 
AAGACGGAGG AGCCACCGCT GGCCCTCTTT GCCTACTGCA CGCCCTTCGG GCCGCCCAGC 
CTGCTGGAAA TCCCGCACAA GGAGAACATG TTCAAGTCCA AGCACAAGCT GGACTTCTCC 
CTGGTATCAA TGGACCAGCG CGGCAAGCAC ATCCTGGGCT ACGCGGACGC CGAGCTGGTC 
AACATGGGCG GCTACGATCT GGTGCACTAC GATGACCTTG CCTATGTGGC CAGCGCCCAT 
CAGGAGCTTT TGAAGACGGG TGCCTCTGGC ATGATCGCCT ACCGTTACCA AAAGAAGGAT 
GGCGAGTGGC AATGGCTGCA GACGAGCTCG CGCCTGGTCT ACAAGAACTC CAAGCCGGAC 
TTTGTGATCT GTACGCACCG CCAGCTGATG GACGAGGAGG GCCACGATCT GCTGGGCAAG 
CGCACCATGG ACTTTAAGGT CAGCTACCTG GACACGGGAC TGGCGTCCAC CTACTTCTCC 
GAGGCGGACC AGCTGGTAGT GCCTCCGAGC ACCTCCCCCA CGGCCCACGC TCTGCCGCCG 
CCGGTGACGC CAACGCGTCC AAATCGCCGC TACAAGACGC AGTTGCGCGA CTTCCTTTCC 
ACTTGTCGCA GCAAACGCAA GCTCCAGCAG CAGAACCAAC CACAGACGCA GCAAACCTCA 
CCACTTGGTG GCCAGGTGGG ATCCCCTGCT CCGGCTGTAG CCGTGGAGTA CTTGCCCGAT 
CCGGCAGCTG CAGTGGCCGC TGCCTACTCC AACCTAAATC CCATGTACAC AACCTCGCCG 
TATGCCAGTG CCGCGGACAA TCTATACATG GGTAGCTCCA TGCCGGCCAA CGCCTTCTAC 
CCAGTCAGCG AGAACCTCTT CCATCAGTAC CGGCTGCAGG GCGCCGTCGG TGGCTACTAC 
ACGGATTATC CCCACTCCGG CGCTCCAGCC TCAGCTTACG TAGCTAATGG ATTTCTCTCT 
TACGACGGAT ACGCCATTGC TTCCAAAGCG GACGAAAAGT GGCAAGAGAC TGGAAAGTAC 
TACAGTGGCT ACAGCAGTGG CTATGGAAGT CCAACATCTA CGCCACAGCA AATTCCTCTG 
AAGACGCCGA AATCTTCACC CCAGGTGATG GAGGTGATAT CTTGCTCCTC GGACGGTCCA 
TCACCTGTGG GCGGAGCCAC GCCCAACGGA GTCGGAAGCG TTACCCCCAA AGTTGAGTTG 
GCCGGAACAA CGGCGGCAGC AGCAGCGGGT CAAGATCCCT ACGAGCGTCA AACGGTGCTG 
ATGTGGGGCA CCACGCACTC GAGTGGGGTA CCGCTAAATA GTCATGGCGG TCGAACTGGA 
GCCGGAAACC CTGCCTCACC ACAGCGATCC ACTCCTCTTG GCAACGGATT GGGCTACGCA 
AGCGCCAATA ATAACAACAA CGATCTTGAA CCTGCCACGG CTGCCAAGTG GAACGGAACA 
AAGGAGCTGC CGGGCAAGTC GGGCAGTGCC AGTACGCCGG AGAGCTACCA GATGCAGCAT 
GATGATTCCG GCCTTTACTC CGCCTCTTCG CACACAACCT CTCCGCAGCA GCAACAACAG 
CAGCAGCTCC AACAATCACA GCGAGGAGTT GGTTCCAATG TTAGCGCTCC CAGCAGCTCA 
CTCACTAGCA CGGGCACAGA TCAGCAGGCG GTGCACCCAT CCAGTTGCCA TCAACAACAG 
CAGCAGCAGC AGCAACAACA CCATCACGCC CACCCGCATC CGCACTCGCA TCATCACCAC 
CATCATCACC ATCACCATGA GACGGCGCAT CAGCACAGCA GCGAGATCAT TCTCACCACC 
CATCATCAGC AACAGTCGCA GCAGCAGCAG CAATCGCTCG CTGGCTACCC GCTGCACCAC 
CAGCTGGTGG GAGTTGGATC AGGATCACCG CCGCCGCAGC CGCCAGTCGC ATTGTCCCGC 
TCGCCCACGA CCTTGCCGGC GGTCAGCAGC TTGCTGAATG GATCGGCTTC AGCAGGAGGA 
GCATCGGATA TTCCATACCA GCAATTGGCC ATTGTGTCCG CTCCTCTGGT GATTTGCGCC 
GAGGAGGTGG GTGGAGGCGT CAGCAGCATT GGCCGCAAGT CATCCAAGCA GCAACAGTCC 
CTGCAACACC TGCAGCAGCA ACAGTCACTG CAACAGCAGC AACATGCAAT TTATCAGCAG 
CAACATCACC ACTCGCAGCA TCATCCGCAT CATCAGCTGC TCTATCCGGC GAACATTACG 
GCGCACACCG CGCTGGCTTA TGCCCCGCCC ACGGCCGGAG GCGCATATGC CGATGGCAGT 
CCGCTGCTCT CCTTCTCCGA GGTGACCAAC ACGCTACTGA ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247140.1
CDS	723..3770
Translation	MSQLGTVYATKRRRRNGKSLKPPPKDGVTKSNPSKRHRERLNAELDLLASLLPFEQNILSKLDRLSILRLSVSYLRTKSYFQVVMHKDKEDNGVLPHIHAHDGYRTRELGAFEHGLLDGDMFLQALNGFLMILTCEGEVFFATHSIESYLGFHQSDIVHQSVYELVHSEDREELQRQLLWNSFLPADMSSMQLAETLAPDKALYLERSFTVRFRCLLDNTSGFLRLDIRGRIKVLHGQNRKTEEPPLALFAYCTPFGPPSLLEIPHKENMFKSKHKLDFSLVSMDQRGKHILGYADAELVNMGGYDLVHYDDLAYVASAHQELLKTGASGMIAYRYQKKDGEWQWLQTSSRLVYKNSKPDFVICTHRQLMDEEGHDLLGKRTMDFKVSYLDTGLASTYFSEADQLVVPPSTSPTAHALPPPVTPTRPNRRYKTQLRDFLSTCRSKRKLQQQNQPQTQQTSPLGGQVGSPAPAVAVEYLPDPAAAVAAAYSNLNPMYTTSPYASAADNLYMGSSMPANAFYPVSENLFHQYRLQGAVGGYYTDYPHSGAPASAYVANGFLSYDGYAIASKADEKWQETGKYYSGYSSGYGSPTSTPQQIPLKTPKSSPQVMEVISCSSDGPSPVGGATPNGVGSVTPKVELAGTTAAAAAGQDPYERQTVLMWGTTHSSGVPLNSHGGRTGAGNPASPQRSTPLGNGLGYASANNNNNDLEPATAAKWNGTKELPGKSGSASTPESYQMQHDDSGLYSASSHTTSPQQQQQQQLQQSQRGVGSNVSAPSSSLTSTGTDQQAVHPSSCHQQQQQQQQQHHHAHPHPHSHHHHHHHHHHETAHQHSSEIILTTHHQQQSQQQQQSLAGYPLHHQLVGVGSGSPPPQPPVALSRSPTTLPAVSSLLNGSASAGGASDIPYQQLAIVSAPLVICAEEVGGGVSSIGRKSSKQQQSLQHLQQQQSLQQQQHAIYQQQHHHSQHHPHHQLLYPANITAHTALAYAPPTAGGAYADGSPLLSFSEVTNTLLNQ

Target ORF information:

RefSeq Version	NM_001260211.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spineless (ss), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260211.2

ORF Insert Sequence:

ATGAGCCAGC TGGGCACCGT CTACGCCACC AAGCGAAGGC GACGCAACGG CAAGAGCCTG 
AAGCCGCCGC CCAAGGATGG CGTCACAAAG AGCAATCCAT CAAAGCGGCA TCGGGAGCGC 
CTCAACGCCG AGCTGGATCT GCTGGCCTCG CTGCTCCCCT TCGAGCAGAA CATCTTGAGC 
AAACTGGATC GACTGAGCAT TCTAAGGCTG TCTGTTAGTT ATTTAAGAAC CAAAAGTTAT 
TTTCAAGTTG TTATGCATAA GGATAAGGAG GATAACGGAG TCCTGCCCCA CATACACGCA 
CATGACGGCT ACAGGACACG AGAACTAGGC GCCTTCGAGC ACGGCCTGTT GGATGGTGAT 
ATGTTCCTCC AGGCCCTAAA TGGATTTCTA ATGATACTGA CATGCGAAGG CGAAGTCTTC 
TTTGCCACGC ACAGCATCGA GAGCTATTTG GGTTTTCATC AGTCGGACAT CGTCCACCAG 
TCGGTGTACG AACTGGTGCA CTCGGAGGAC CGCGAGGAGC TGCAGCGCCA GCTGCTGTGG 
AACAGTTTCC TGCCCGCCGA CATGTCCAGC ATGCAGCTGG CGGAGACCCT GGCGCCGGAC 
AAGGCGCTCT ACCTGGAGCG CAGCTTCACC GTCCGCTTCC GCTGCCTGCT GGACAACACG 
AGCGGCTTCC TGCGCCTGGA CATCCGTGGC CGCATCAAGG TCCTGCATGG CCAGAACCGC 
AAGACGGAGG AGCCACCGCT GGCCCTCTTT GCCTACTGCA CGCCCTTCGG GCCGCCCAGC 
CTGCTGGAAA TCCCGCACAA GGAGAACATG TTCAAGTCCA AGCACAAGCT GGACTTCTCC 
CTGGTATCAA TGGACCAGCG CGGCAAGCAC ATCCTGGGCT ACGCGGACGC CGAGCTGGTC 
AACATGGGCG GCTACGATCT GGTGCACTAC GATGACCTTG CCTATGTGGC CAGCGCCCAT 
CAGGAGCTTT TGAAGACGGG TGCCTCTGGC ATGATCGCCT ACCGTTACCA AAAGAAGGAT 
GGCGAGTGGC AATGGCTGCA GACGAGCTCG CGCCTGGTCT ACAAGAACTC CAAGCCGGAC 
TTTGTGATCT GTACGCACCG CCAGCTGATG GACGAGGAGG GCCACGATCT GCTGGGCAAG 
CGCACCATGG ACTTTAAGGT CAGCTACCTG GACACGGGAC TGGCGTCCAC CTACTTCTCC 
GAGGCGGACC AGCTGGTAGT GCCTCCGAGC ACCTCCCCCA CGGCCCACGC TCTGCCGCCG 
CCGGTGACGC CAACGCGTCC AAATCGCCGC TACAAGACGC AGTTGCGCGA CTTCCTTTCC 
ACTTGTCGCA GCAAACGCAA GCTCCAGCAG CAGAACCAAC CACAGACGCA GCAAACCTCA 
CCACTTGGTG GCCAGGTGGG ATCCCCTGCT CCGGCTGTAG CCGTGGAGTA CTTGCCCGAT 
CCGGCAGCTG CAGTGGCCGC TGCCTACTCC AACCTAAATC CCATGTACAC AACCTCGCCG 
TATGCCAGTG CCGCGGACAA TCTATACATG GGTAGCTCCA TGCCGGCCAA CGCCTTCTAC 
CCAGTCAGCG AGAACCTCTT CCATCAGTAC CGGCTGCAGG GCGCCGTCGG TGGCTACTAC 
ACGGATTATC CCCACTCCGG CGCTCCAGCC TCAGCTTACG TAGCTAATGG ATTTCTCTCT 
TACGACGGAT ACGCCATTGC TTCCAAAGCG GACGAAAAGT GGCAAGAGAC TGGAAAGTAC 
TACAGTGGCT ACAGCAGTGG CTATGGAAGT CCAACATCTA CGCCACAGCA AATTCCTCTG 
AAGACGCCGA AATCTTCACC CCAGGTGATG GAGGTGATAT CTTGCTCCTC GGACGGTCCA 
TCACCTGTGG GCGGAGCCAC GCCCAACGGA GTCGGAAGCG TTACCCCCAA AGTTGAGTTG 
GCCGGAACAA CGGCGGCAGC AGCAGCGGGT CAAGATCCCT ACGAGCGTCA AACGGTGCTG 
ATGTGGGGCA CCACGCACTC GAGTGGGGTA CCGCTAAATA GTCATGGCGG TCGAACTGGA 
GCCGGAAACC CTGCCTCACC ACAGCGATCC ACTCCTCTTG GCAACGGATT GGGCTACGCA 
AGCGCCAATA ATAACAACAA CGATCTTGAA CCTGCCACGG CTGCCAAGTG GAACGGAACA 
AAGGAGCTGC CGGGCAAGTC GGGCAGTGCC AGTACGCCGG AGAGCTACCA GATGCAGCAT 
GATGATTCCG GCCTTTACTC CGCCTCTTCG CACACAACCT CTCCGCAGCA GCAACAACAG 
CAGCAGCTCC AACAATCACA GCGAGGAGTT GGTTCCAATG TTAGCGCTCC CAGCAGCTCA 
CTCACTAGCA CGGGCACAGA TCAGCAGGCG GTGCACCCAT CCAGTTGCCA TCAACAACAG 
CAGCAGCAGC AGCAACAACA CCATCACGCC CACCCGCATC CGCACTCGCA TCATCACCAC 
CATCATCACC ATCACCATGA GACGGCGCAT CAGCACAGCA GCGAGATCAT TCTCACCACC 
CATCATCAGC AACAGTCGCA GCAGCAGCAG CAATCGCTCG CTGGCTACCC GCTGCACCAC 
CAGCTGGTGG GAGTTGGATC AGGATCACCG CCGCCGCAGC CGCCAGTCGC ATTGTCCCGC 
TCGCCCACGA CCTTGCCGGC GGTCAGCAGC TTGCTGAATG GATCGGCTTC AGCAGGAGGA 
GCATCGGATA TTCCATACCA GCAATTGGCC ATTGTGTCCG CTCCTCTGGT GATTTGCGCC 
GAGGAGGTGG GTGGAGGCGT CAGCAGCATT GGCCGCAAGT CATCCAAGCA GCAACAGTCC 
CTGCAACACC TGCAGCAGCA ACAGTCACTG CAACAGCAGC AACATGCAAT TTATCAGCAG 
CAACATCACC ACTCGCAGCA TCATCCGCAT CATCAGCTGC TCTATCCGGC GAACATTACG 
GCGCACACCG CGCTGGCTTA TGCCCCGCCC ACGGCCGGAG GCGCATATGC CGATGGCAGT 
CCGCTGCTCT CCTTCTCCGA GGTGACCAAC ACGCTACTGA ACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ss cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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