pxb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pxb
Entrez Gene ID	41899
Full Name	CG33207 gene product from transcript CG33207-RC
Synonyms	BcDNA:RE16319,CG14873,CG14874,CG33207,CG33207/Pxb,Dmel\CG33207,Pxb
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89A1-89A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_176504.2	NP_788681.1	pxb, isoform B
NM_001275680.1	NP_001262609.1	pxb, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mid-to-late third instar leg discs, high levels of Hedgehog signals repress the transcription of pxb, a newly identified gene encoding a transmembrane protein expressed specifically in the anterior compartment.
Title: Requirements of high levels of Hedgehog signaling activity for medial-region cell fate determination in Drosophila legs: identification of pxb, a putative Hedgehog signaling attenuator gene repressed along the anterior-posterior compartment boundary.

The following pxb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pxb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05025	NM_176504.2 Latest version!	Drosophila melanogaster pxb, transcript variant B (pxb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05025	NM_001275680.1 Latest version!	Drosophila melanogaster pxb, transcript variant C (pxb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pxb ( NM_176504.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788681.1 Drosophila melanogaster pxb, transcript variant B (pxb), mRNA.

Price & Availability↑

CloneID	OFa05025
Clone ID Related Accession (Same CDS sequence)	NM_176504.2 , NM_001275680.1
Accession Version	NM_176504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pxb, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176504.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAAAA TGCTGGATAT GATGGAACTG TCCGAAGCAG AGCTCTTCAT GTTCGACTGC 
GACGATTGCT CGGGCACGCC CCTTGCCCCA CCGCCCCAGT TCCTCATCCC ACCGCCACCA 
AGACCGCCGG CCCTGGCCGA ATTCACGGTG GTGCCGCAGG ATTGCAACGA GGAGCAATTG 
GCCCAGCAGC AATGGGAGAG CGATGTCTGC CAGGCAATGC CGATCTTGGA TGCCAGCCTG 
TACAGCAGTC AGTCGTTGGC CACATCGGCC ATGATAGCCG TGTTCTCGCT GCTCTTGGTC 
TTGATTGTTC TCATCTCGTC GCTGTTCGTT TGGAAAAACA AGCGGAAGGT GCAGAATCTG 
CTGCCCTGCA AGACACCCAC ACGAGGCCCC CTCAATGGAG CCATGCCCCT GGGCGGTGGC 
ACTTTGGGAT CGGCGCACCA CCATCATCAT CACGGGATGT ACGAGGATCC TGATGCGCAC 
TTGGGCCACC ATAGACCCCT GGTGATGCAT CGGCATCATC ATCACCAGCA GCTGGCGCAC 
CATCATCACT ATCAGCAGCA TCACTCCGAG CTGCTCCAAG GAAAGAGTAT TCACTATCCC 
AGCGGCTATC CCATGACGCG TTCGCCACCG TTCTTGGTCA GCTCCTCACC TGGACCCGAT 
CCCTATCGCT CCAATGACAA TGTCTACGAG GAACTGGGAC CGGGACGCGT TGATTCCGAT 
GGCGAGTCGG AGCCGCCACT TCATTCAGAT GACGATTTCG CCGAAGATGA ACTATCCCTG 
CCTGGCGAAT GCAGTTTCCA GAAGGAAACC AACCTGCCAG CGGCAGCCGG TGCCACGCCC 
TATCACGAGA GGGCGGCGGG AAGCAGCAGC AGTGGCACAG GAGTAGCAGG AGTAGCGGGA 
GCCACTGGAT CAGTGGCTAC ATCCGCGAGT CTGCCACAGG CTGTTCAGGA GCGTCACAGT 
GTCCTGAGCA GTGGTTCCTC AATGGGTCAG GACACGTCCA CCGCGACAGC ATCCTCTGCC 
AGCAATAACA ATCACGAGTT GGCATCGGGA TCGAACAATC GCCGTACTGG CCGCACGCGA 
CAAAGTCGCA GCTCTCAGGA TCAGGATCTC AACAACAGCA CCTCCACGGC GGATATTGCC 
GACCTGGCGC CTCTGTACGA CAACCGCAGC AATCTGATGT CCCTGGGCTA CGTGGCTCGC 
TCCACAGCAC CGCAATTCTA TAACACCTTG GAGCACGAAG TGGAGCGCAG AAATCGGATT 
AATGCGCAAC TCAGCAAAGC CCCAGTTCCT CCGCTATCCA CGATTTACAG GGAACGCATC 
CGCTATCCCC ATGCCAGCCA GCAGTATCCG TCGAGTGTGG CCAGTGGCCT GATGGCCGCC 
ACGATGAGGG CGAGAACTCA ACCGAGGGTA TTGGACCGTC GATTAGCACC ACATCAGCAT 
CAGCACCACC GCATACACGC TGCACCAATG GCCCAGGAGC CATCCTACTG CTACGCAGAG 
CCCATGTACA ATGCCAACTA TTACTACGAA AGTGCTGGTG GCGCCCGGCC TTATGCCTCC 
ACCTTACTAC CCACTGCTGC GGACTATAGG CATCCGTACG GGGACTCCAG TTTTGGATCC 
GATTCGGGAT ACAGCCACCA TACGCCTGCC AGCTCGATTG GGCGACAGGA CTATGACGTA 
GCCGAGCCAC AAGTGCTAAC CCAACCGAAA CCAAGCAAAC TTAGTTCCGC CCTAAACTTC 
AGTTGGAATC GCAACTCGCG GAGCAAGGGA ACAAGTGGAG GAGCTATTGC CAAGACCTCG 
GGAAATAGCA GCAACAGTAG TGCCACCAGC CCCAACAACA ACAGCAGCGC CTGCTCCACC 
GTCACATCCC AATTGGAAAA CAACAATACT GCCAGTCTCT CACCAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788681.1
CDS	599..2509
Translation	MWKMLDMMELSEAELFMFDCDDCSGTPLAPPPQFLIPPPPRPPALAEFTVVPQDCNEEQLAQQQWESDVCQAMPILDASLYSSQSLATSAMIAVFSLLLVLIVLISSLFVWKNKRKVQNLLPCKTPTRGPLNGAMPLGGGTLGSAHHHHHHGMYEDPDAHLGHHRPLVMHRHHHHQQLAHHHHYQQHHSELLQGKSIHYPSGYPMTRSPPFLVSSSPGPDPYRSNDNVYEELGPGRVDSDGESEPPLHSDDDFAEDELSLPGECSFQKETNLPAAAGATPYHERAAGSSSSGTGVAGVAGATGSVATSASLPQAVQERHSVLSSGSSMGQDTSTATASSASNNNHELASGSNNRRTGRTRQSRSSQDQDLNNSTSTADIADLAPLYDNRSNLMSLGYVARSTAPQFYNTLEHEVERRNRINAQLSKAPVPPLSTIYRERIRYPHASQQYPSSVASGLMAATMRARTQPRVLDRRLAPHQHQHHRIHAAPMAQEPSYCYAEPMYNANYYYESAGGARPYASTLLPTAADYRHPYGDSSFGSDSGYSHHTPASSIGRQDYDVAEPQVLTQPKPSKLSSALNFSWNRNSRSKGTSGGAIAKTSGNSSNSSATSPNNNSSACSTVTSQLENNNTASLSPA

Target ORF information:

RefSeq Version	NM_176504.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pxb, transcript variant B (pxb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176504.2

ORF Insert Sequence:

ATGTGGAAAA TGCTGGATAT GATGGAACTG TCCGAAGCAG AGCTCTTCAT GTTCGACTGC 
GACGATTGCT CGGGCACGCC CCTTGCCCCA CCGCCCCAGT TCCTCATCCC ACCGCCACCA 
AGACCGCCGG CCCTGGCCGA ATTCACGGTG GTGCCGCAGG ATTGCAACGA GGAGCAATTG 
GCCCAGCAGC AATGGGAGAG CGATGTCTGC CAGGCAATGC CGATCTTGGA TGCCAGCCTG 
TACAGCAGTC AGTCGTTGGC CACATCGGCC ATGATAGCCG TGTTCTCGCT GCTCTTGGTC 
TTGATTGTTC TCATCTCGTC GCTGTTCGTT TGGAAAAACA AGCGGAAGGT GCAGAATCTG 
CTGCCCTGCA AGACACCCAC ACGAGGCCCC CTCAATGGAG CCATGCCCCT GGGCGGTGGC 
ACTTTGGGAT CGGCGCACCA CCATCATCAT CACGGGATGT ACGAGGATCC TGATGCGCAC 
TTGGGCCACC ATAGACCCCT GGTGATGCAT CGGCATCATC ATCACCAGCA GCTGGCGCAC 
CATCATCACT ATCAGCAGCA TCACTCCGAG CTGCTCCAAG GAAAGAGTAT TCACTATCCC 
AGCGGCTATC CCATGACGCG TTCGCCACCG TTCTTGGTCA GCTCCTCACC TGGACCCGAT 
CCCTATCGCT CCAATGACAA TGTCTACGAG GAACTGGGAC CGGGACGCGT TGATTCCGAT 
GGCGAGTCGG AGCCGCCACT TCATTCAGAT GACGATTTCG CCGAAGATGA ACTATCCCTG 
CCTGGCGAAT GCAGTTTCCA GAAGGAAACC AACCTGCCAG CGGCAGCCGG TGCCACGCCC 
TATCACGAGA GGGCGGCGGG AAGCAGCAGC AGTGGCACAG GAGTAGCAGG AGTAGCGGGA 
GCCACTGGAT CAGTGGCTAC ATCCGCGAGT CTGCCACAGG CTGTTCAGGA GCGTCACAGT 
GTCCTGAGCA GTGGTTCCTC AATGGGTCAG GACACGTCCA CCGCGACAGC ATCCTCTGCC 
AGCAATAACA ATCACGAGTT GGCATCGGGA TCGAACAATC GCCGTACTGG CCGCACGCGA 
CAAAGTCGCA GCTCTCAGGA TCAGGATCTC AACAACAGCA CCTCCACGGC GGATATTGCC 
GACCTGGCGC CTCTGTACGA CAACCGCAGC AATCTGATGT CCCTGGGCTA CGTGGCTCGC 
TCCACAGCAC CGCAATTCTA TAACACCTTG GAGCACGAAG TGGAGCGCAG AAATCGGATT 
AATGCGCAAC TCAGCAAAGC CCCAGTTCCT CCGCTATCCA CGATTTACAG GGAACGCATC 
CGCTATCCCC ATGCCAGCCA GCAGTATCCG TCGAGTGTGG CCAGTGGCCT GATGGCCGCC 
ACGATGAGGG CGAGAACTCA ACCGAGGGTA TTGGACCGTC GATTAGCACC ACATCAGCAT 
CAGCACCACC GCATACACGC TGCACCAATG GCCCAGGAGC CATCCTACTG CTACGCAGAG 
CCCATGTACA ATGCCAACTA TTACTACGAA AGTGCTGGTG GCGCCCGGCC TTATGCCTCC 
ACCTTACTAC CCACTGCTGC GGACTATAGG CATCCGTACG GGGACTCCAG TTTTGGATCC 
GATTCGGGAT ACAGCCACCA TACGCCTGCC AGCTCGATTG GGCGACAGGA CTATGACGTA 
GCCGAGCCAC AAGTGCTAAC CCAACCGAAA CCAAGCAAAC TTAGTTCCGC CCTAAACTTC 
AGTTGGAATC GCAACTCGCG GAGCAAGGGA ACAAGTGGAG GAGCTATTGC CAAGACCTCG 
GGAAATAGCA GCAACAGTAG TGCCACCAGC CCCAACAACA ACAGCAGCGC CTGCTCCACC 
GTCACATCCC AATTGGAAAA CAACAATACT GCCAGTCTCT CACCAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pxb ( NM_001275680.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262609.1 Drosophila melanogaster pxb, transcript variant C (pxb), mRNA.

Price & Availability↑

CloneID	OFa05025
Clone ID Related Accession (Same CDS sequence)	NM_176504.2 , NM_001275680.1
Accession Version	NM_001275680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pxb, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAAAA TGCTGGATAT GATGGAACTG TCCGAAGCAG AGCTCTTCAT GTTCGACTGC 
GACGATTGCT CGGGCACGCC CCTTGCCCCA CCGCCCCAGT TCCTCATCCC ACCGCCACCA 
AGACCGCCGG CCCTGGCCGA ATTCACGGTG GTGCCGCAGG ATTGCAACGA GGAGCAATTG 
GCCCAGCAGC AATGGGAGAG CGATGTCTGC CAGGCAATGC CGATCTTGGA TGCCAGCCTG 
TACAGCAGTC AGTCGTTGGC CACATCGGCC ATGATAGCCG TGTTCTCGCT GCTCTTGGTC 
TTGATTGTTC TCATCTCGTC GCTGTTCGTT TGGAAAAACA AGCGGAAGGT GCAGAATCTG 
CTGCCCTGCA AGACACCCAC ACGAGGCCCC CTCAATGGAG CCATGCCCCT GGGCGGTGGC 
ACTTTGGGAT CGGCGCACCA CCATCATCAT CACGGGATGT ACGAGGATCC TGATGCGCAC 
TTGGGCCACC ATAGACCCCT GGTGATGCAT CGGCATCATC ATCACCAGCA GCTGGCGCAC 
CATCATCACT ATCAGCAGCA TCACTCCGAG CTGCTCCAAG GAAAGAGTAT TCACTATCCC 
AGCGGCTATC CCATGACGCG TTCGCCACCG TTCTTGGTCA GCTCCTCACC TGGACCCGAT 
CCCTATCGCT CCAATGACAA TGTCTACGAG GAACTGGGAC CGGGACGCGT TGATTCCGAT 
GGCGAGTCGG AGCCGCCACT TCATTCAGAT GACGATTTCG CCGAAGATGA ACTATCCCTG 
CCTGGCGAAT GCAGTTTCCA GAAGGAAACC AACCTGCCAG CGGCAGCCGG TGCCACGCCC 
TATCACGAGA GGGCGGCGGG AAGCAGCAGC AGTGGCACAG GAGTAGCAGG AGTAGCGGGA 
GCCACTGGAT CAGTGGCTAC ATCCGCGAGT CTGCCACAGG CTGTTCAGGA GCGTCACAGT 
GTCCTGAGCA GTGGTTCCTC AATGGGTCAG GACACGTCCA CCGCGACAGC ATCCTCTGCC 
AGCAATAACA ATCACGAGTT GGCATCGGGA TCGAACAATC GCCGTACTGG CCGCACGCGA 
CAAAGTCGCA GCTCTCAGGA TCAGGATCTC AACAACAGCA CCTCCACGGC GGATATTGCC 
GACCTGGCGC CTCTGTACGA CAACCGCAGC AATCTGATGT CCCTGGGCTA CGTGGCTCGC 
TCCACAGCAC CGCAATTCTA TAACACCTTG GAGCACGAAG TGGAGCGCAG AAATCGGATT 
AATGCGCAAC TCAGCAAAGC CCCAGTTCCT CCGCTATCCA CGATTTACAG GGAACGCATC 
CGCTATCCCC ATGCCAGCCA GCAGTATCCG TCGAGTGTGG CCAGTGGCCT GATGGCCGCC 
ACGATGAGGG CGAGAACTCA ACCGAGGGTA TTGGACCGTC GATTAGCACC ACATCAGCAT 
CAGCACCACC GCATACACGC TGCACCAATG GCCCAGGAGC CATCCTACTG CTACGCAGAG 
CCCATGTACA ATGCCAACTA TTACTACGAA AGTGCTGGTG GCGCCCGGCC TTATGCCTCC 
ACCTTACTAC CCACTGCTGC GGACTATAGG CATCCGTACG GGGACTCCAG TTTTGGATCC 
GATTCGGGAT ACAGCCACCA TACGCCTGCC AGCTCGATTG GGCGACAGGA CTATGACGTA 
GCCGAGCCAC AAGTGCTAAC CCAACCGAAA CCAAGCAAAC TTAGTTCCGC CCTAAACTTC 
AGTTGGAATC GCAACTCGCG GAGCAAGGGA ACAAGTGGAG GAGCTATTGC CAAGACCTCG 
GGAAATAGCA GCAACAGTAG TGCCACCAGC CCCAACAACA ACAGCAGCGC CTGCTCCACC 
GTCACATCCC AATTGGAAAA CAACAATACT GCCAGTCTCT CACCAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262609.1
CDS	599..2509
Translation	MWKMLDMMELSEAELFMFDCDDCSGTPLAPPPQFLIPPPPRPPALAEFTVVPQDCNEEQLAQQQWESDVCQAMPILDASLYSSQSLATSAMIAVFSLLLVLIVLISSLFVWKNKRKVQNLLPCKTPTRGPLNGAMPLGGGTLGSAHHHHHHGMYEDPDAHLGHHRPLVMHRHHHHQQLAHHHHYQQHHSELLQGKSIHYPSGYPMTRSPPFLVSSSPGPDPYRSNDNVYEELGPGRVDSDGESEPPLHSDDDFAEDELSLPGECSFQKETNLPAAAGATPYHERAAGSSSSGTGVAGVAGATGSVATSASLPQAVQERHSVLSSGSSMGQDTSTATASSASNNNHELASGSNNRRTGRTRQSRSSQDQDLNNSTSTADIADLAPLYDNRSNLMSLGYVARSTAPQFYNTLEHEVERRNRINAQLSKAPVPPLSTIYRERIRYPHASQQYPSSVASGLMAATMRARTQPRVLDRRLAPHQHQHHRIHAAPMAQEPSYCYAEPMYNANYYYESAGGARPYASTLLPTAADYRHPYGDSSFGSDSGYSHHTPASSIGRQDYDVAEPQVLTQPKPSKLSSALNFSWNRNSRSKGTSGGAIAKTSGNSSNSSATSPNNNSSACSTVTSQLENNNTASLSPA

Target ORF information:

RefSeq Version	NM_001275680.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pxb, transcript variant C (pxb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275680.1

ORF Insert Sequence:

ATGTGGAAAA TGCTGGATAT GATGGAACTG TCCGAAGCAG AGCTCTTCAT GTTCGACTGC 
GACGATTGCT CGGGCACGCC CCTTGCCCCA CCGCCCCAGT TCCTCATCCC ACCGCCACCA 
AGACCGCCGG CCCTGGCCGA ATTCACGGTG GTGCCGCAGG ATTGCAACGA GGAGCAATTG 
GCCCAGCAGC AATGGGAGAG CGATGTCTGC CAGGCAATGC CGATCTTGGA TGCCAGCCTG 
TACAGCAGTC AGTCGTTGGC CACATCGGCC ATGATAGCCG TGTTCTCGCT GCTCTTGGTC 
TTGATTGTTC TCATCTCGTC GCTGTTCGTT TGGAAAAACA AGCGGAAGGT GCAGAATCTG 
CTGCCCTGCA AGACACCCAC ACGAGGCCCC CTCAATGGAG CCATGCCCCT GGGCGGTGGC 
ACTTTGGGAT CGGCGCACCA CCATCATCAT CACGGGATGT ACGAGGATCC TGATGCGCAC 
TTGGGCCACC ATAGACCCCT GGTGATGCAT CGGCATCATC ATCACCAGCA GCTGGCGCAC 
CATCATCACT ATCAGCAGCA TCACTCCGAG CTGCTCCAAG GAAAGAGTAT TCACTATCCC 
AGCGGCTATC CCATGACGCG TTCGCCACCG TTCTTGGTCA GCTCCTCACC TGGACCCGAT 
CCCTATCGCT CCAATGACAA TGTCTACGAG GAACTGGGAC CGGGACGCGT TGATTCCGAT 
GGCGAGTCGG AGCCGCCACT TCATTCAGAT GACGATTTCG CCGAAGATGA ACTATCCCTG 
CCTGGCGAAT GCAGTTTCCA GAAGGAAACC AACCTGCCAG CGGCAGCCGG TGCCACGCCC 
TATCACGAGA GGGCGGCGGG AAGCAGCAGC AGTGGCACAG GAGTAGCAGG AGTAGCGGGA 
GCCACTGGAT CAGTGGCTAC ATCCGCGAGT CTGCCACAGG CTGTTCAGGA GCGTCACAGT 
GTCCTGAGCA GTGGTTCCTC AATGGGTCAG GACACGTCCA CCGCGACAGC ATCCTCTGCC 
AGCAATAACA ATCACGAGTT GGCATCGGGA TCGAACAATC GCCGTACTGG CCGCACGCGA 
CAAAGTCGCA GCTCTCAGGA TCAGGATCTC AACAACAGCA CCTCCACGGC GGATATTGCC 
GACCTGGCGC CTCTGTACGA CAACCGCAGC AATCTGATGT CCCTGGGCTA CGTGGCTCGC 
TCCACAGCAC CGCAATTCTA TAACACCTTG GAGCACGAAG TGGAGCGCAG AAATCGGATT 
AATGCGCAAC TCAGCAAAGC CCCAGTTCCT CCGCTATCCA CGATTTACAG GGAACGCATC 
CGCTATCCCC ATGCCAGCCA GCAGTATCCG TCGAGTGTGG CCAGTGGCCT GATGGCCGCC 
ACGATGAGGG CGAGAACTCA ACCGAGGGTA TTGGACCGTC GATTAGCACC ACATCAGCAT 
CAGCACCACC GCATACACGC TGCACCAATG GCCCAGGAGC CATCCTACTG CTACGCAGAG 
CCCATGTACA ATGCCAACTA TTACTACGAA AGTGCTGGTG GCGCCCGGCC TTATGCCTCC 
ACCTTACTAC CCACTGCTGC GGACTATAGG CATCCGTACG GGGACTCCAG TTTTGGATCC 
GATTCGGGAT ACAGCCACCA TACGCCTGCC AGCTCGATTG GGCGACAGGA CTATGACGTA 
GCCGAGCCAC AAGTGCTAAC CCAACCGAAA CCAAGCAAAC TTAGTTCCGC CCTAAACTTC 
AGTTGGAATC GCAACTCGCG GAGCAAGGGA ACAAGTGGAG GAGCTATTGC CAAGACCTCG 
GGAAATAGCA GCAACAGTAG TGCCACCAGC CCCAACAACA ACAGCAGCGC CTGCTCCACC 
GTCACATCCC AATTGGAAAA CAACAATACT GCCAGTCTCT CACCAGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pxb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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