ea cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ea
Entrez Gene ID	41858
Full Name	easter
Synonyms	CG4920,Dmel\CG4920,EA,SP24,c-SP24
General protein information	Preferred Names easter Names CG4920 gene product from transcript CG4920-RB CG4920-PA CG4920-PB ea-PA ea-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88F1-88F1\|3-57 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079638.3	NP_524362.2	easter, isoform A
NM_001275668.1	NP_001262597.1	easter, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	CLIPB5	XP_313032.4
Drosophila melanogaster (fruit fly)	ea	NP_524362.2
Drosophila melanogaster (fruit fly)	MP1	NP_649450.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study reports the expression and refolding of Easter, and show that the renatured enzyme performs the activation cleavage of two spatzle isoforms.
Title: In vitro maturation of Drosophila melanogaster Sp?tzle protein with refolded Easter reveals a novel cleavage site within the prodomain.

observation suggests a mechanism in which the sulfated target of Pipe promotes a productive interaction between Snake and Easter, perhaps by facilitating conformational changes in a complex containing the two proteins
Title: Pipe-dependent ventral processing of Easter by Snake is the defining step in Drosophila embryo DV axis formation.

provision of three-dimensional model and role of cation binding sites in embryo patterning
Title: Three-dimensional models of proteases involved in patterning of the Drosophila Embryo. Crucial role of predicted cation binding sites.

Easter plays a role in generating the wild-type shape of the Dorsal gradient in the developing Drosophila embryo
Title: Regulation of Easter activity is required for shaping the Dorsal gradient in the Drosophila embryo.

The following ea gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ea cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04977	NM_079638.3 Latest version!	Drosophila melanogaster easter (ea), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08397	NM_001275668.1 Latest version!	Drosophila melanogaster easter (ea), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ea ( NM_079638.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524362.2 Drosophila melanogaster easter (ea), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04977
Clone ID Related Accession (Same CDS sequence)	NM_079638.3
Accession Version	NM_079638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	easter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079638.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAAAGC CATCGATTAT CTGCCTCTTT TTGGGCATTT TGGCGAAATC ATCGGCGGGC 
CAGTTCTACT TCCCCAACGA GGCGGCCCAG GTGCCCAACT ATGGGCGCTG CATAACGCCG 
AATCGGGAGC GTGCGCTGTG CATCCATCTG GAGGACTGCA AGTACCTATA CGGCCTGCTG 
ACCACCACCC CGCTCCGGGA CACGGACCGG CTTTACCTGA GTCGCAGCCA GTGCGGCTAT 
ACGAACGGCA AGGTGCTCAT CTGCTGTCCG GACCGCTACA GGGAGAGCTC ATCGGAGACG 
ACGCCACCGC CCAAGCCCAA TGTAACCAGC AACAGCCTTC TGCCGCTGCC AGGACAATGC 
GGCAACATCC TGTCCAATCG CATCTATGGC GGAATGAAGA CCAAGATCGA CGAGTTCCCC 
TGGATGGCTC TAATTGAATA CACCAAAAGC CAGGGCAAAA AGGGTCACCA TTGCGGCGGA 
TCCTTGATCA GCACAAGATA TGTGATCACA GCCTCGCATT GTGTCAACGG AAAGGCACTG 
CCCACCGACT GGCGGTTGTC CGGTGTTCGT TTGGGCGAAT GGGACACCAA CACTAATCCC 
GATTGCGAAG TAGATGTGCG CGGCATGAAG GACTGCGCGC CACCGCATCT GGACGTGCCC 
GTGGAGCGTA CTATACCCCA TCCCGACTAT ATACCGGCCT CCAAGAACCA GGTCAACGAC 
ATAGCCCTGC TGCGTCTGGC CCAGCAGGTG GAGTACACCG ACTTTGTGCG ACCCATTTGC 
CTGCCTCTGG ACGTCAATCT GCGGTCGGCC ACCTTCGACG GCATCACCAT GGACGTGGCC 
GGCTGGGGCA AAACGGAGCA GCTGTCGGCC AGCAACCTCA AGCTGAAGGC AGCCGTGGAG 
GGATTCCGGA TGGATGAGTG CCAGAACGTC TACAGCAGCC AGGACATTCT CCTCGAGGAC 
ACACAGATGT GTGCCGGCGG CAAGGAAGGC GTCGACTCCT GTCGCGGTGA CTCCGGGGGA 
CCGCTCATCG GCTTGGACAC CAACAAGGTG AACACCTACT ACTTCCTGGC CGGCGTCGTG 
TCATTTGGAC CGACTCCGTG CGGTCTGGCC GGATGGCCAG GAGTTTACAC GCTGGTGGGC 
AAGTACGTTG ATTGGATACA AAACACTATT GAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524362.2
CDS	205..1383
Translation	MLKPSIICLFLGILAKSSAGQFYFPNEAAQVPNYGRCITPNRERALCIHLEDCKYLYGLLTTTPLRDTDRLYLSRSQCGYTNGKVLICCPDRYRESSSETTPPPKPNVTSNSLLPLPGQCGNILSNRIYGGMKTKIDEFPWMALIEYTKSQGKKGHHCGGSLISTRYVITASHCVNGKALPTDWRLSGVRLGEWDTNTNPDCEVDVRGMKDCAPPHLDVPVERTIPHPDYIPASKNQVNDIALLRLAQQVEYTDFVRPICLPLDVNLRSATFDGITMDVAGWGKTEQLSASNLKLKAAVEGFRMDECQNVYSSQDILLEDTQMCAGGKEGVDSCRGDSGGPLIGLDTNKVNTYYFLAGVVSFGPTPCGLAGWPGVYTLVGKYVDWIQNTIES

Target ORF information:

RefSeq Version	NM_079638.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster easter (ea), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079638.3

ORF Insert Sequence:

ATGCTAAAGC CATCGATTAT CTGCCTCTTT TTGGGCATTT TGGCGAAATC ATCGGCGGGC 
CAGTTCTACT TCCCCAACGA GGCGGCCCAG GTGCCCAACT ATGGGCGCTG CATAACGCCG 
AATCGGGAGC GTGCGCTGTG CATCCATCTG GAGGACTGCA AGTACCTATA CGGCCTGCTG 
ACCACCACCC CGCTCCGGGA CACGGACCGG CTTTACCTGA GTCGCAGCCA GTGCGGCTAT 
ACGAACGGCA AGGTGCTCAT CTGCTGTCCG GACCGCTACA GGGAGAGCTC ATCGGAGACG 
ACGCCACCGC CCAAGCCCAA TGTAACCAGC AACAGCCTTC TGCCGCTGCC AGGACAATGC 
GGCAACATCC TGTCCAATCG CATCTATGGC GGAATGAAGA CCAAGATCGA CGAGTTCCCC 
TGGATGGCTC TAATTGAATA CACCAAAAGC CAGGGCAAAA AGGGTCACCA TTGCGGCGGA 
TCCTTGATCA GCACAAGATA TGTGATCACA GCCTCGCATT GTGTCAACGG AAAGGCACTG 
CCCACCGACT GGCGGTTGTC CGGTGTTCGT TTGGGCGAAT GGGACACCAA CACTAATCCC 
GATTGCGAAG TAGATGTGCG CGGCATGAAG GACTGCGCGC CACCGCATCT GGACGTGCCC 
GTGGAGCGTA CTATACCCCA TCCCGACTAT ATACCGGCCT CCAAGAACCA GGTCAACGAC 
ATAGCCCTGC TGCGTCTGGC CCAGCAGGTG GAGTACACCG ACTTTGTGCG ACCCATTTGC 
CTGCCTCTGG ACGTCAATCT GCGGTCGGCC ACCTTCGACG GCATCACCAT GGACGTGGCC 
GGCTGGGGCA AAACGGAGCA GCTGTCGGCC AGCAACCTCA AGCTGAAGGC AGCCGTGGAG 
GGATTCCGGA TGGATGAGTG CCAGAACGTC TACAGCAGCC AGGACATTCT CCTCGAGGAC 
ACACAGATGT GTGCCGGCGG CAAGGAAGGC GTCGACTCCT GTCGCGGTGA CTCCGGGGGA 
CCGCTCATCG GCTTGGACAC CAACAAGGTG AACACCTACT ACTTCCTGGC CGGCGTCGTG 
TCATTTGGAC CGACTCCGTG CGGTCTGGCC GGATGGCCAG GAGTTTACAC GCTGGTGGGC 
AAGTACGTTG ATTGGATACA AAACACTATT GAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ea ( NM_001275668.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262597.1 Drosophila melanogaster easter (ea), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08397
Clone ID Related Accession (Same CDS sequence)	NM_001275668.1
Accession Version	NM_001275668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	easter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACCA AGATCGACGA GTTCCCCTGG ATGGCTCTAA TTGAATACAC CAAAAGCCAG 
GGCAAAAAGG GTCACCATTG CGGCGGATCC TTGATCAGCA CAAGATATGT GATCACAGCC 
TCGCATTGTG TCAACGGAAA GGCACTGCCC ACCGACTGGC GGTTGTCCGG TGTTCGTTTG 
GGCGAATGGG ACACCAACAC TAATCCCGAT TGCGAAGTAG ATGTGCGCGG CATGAAGGAC 
TGCGCGCCAC CGCATCTGGA CGTGCCCGTG GAGCGTACTA TACCCCATCC CGACTATATA 
CCGGCCTCCA AGAACCAGGT CAACGACATA GCCCTGCTGC GTCTGGCCCA GCAGGTGGAG 
TACACCGACT TTGTGCGACC CATTTGCCTG CCTCTGGACG TCAATCTGCG GTCGGCCACC 
TTCGACGGCA TCACCATGGA CGTGGCCGGC TGGGGCAAAA CGGAGCAGCT GTCGGCCAGC 
AACCTCAAGC TGAAGGCAGC CGTGGAGGGA TTCCGGATGG ATGAGTGCCA GAACGTCTAC 
AGCAGCCAGG ACATTCTCCT CGAGGACACA CAGATGTGTG CCGGCGGCAA GGAAGGCGTC 
GACTCCTGTC GCGGTGACTC CGGGGGACCG CTCATCGGCT TGGACACCAA CAAGGTGAAC 
ACCTACTACT TCCTGGCCGG CGTCGTGTCA TTTGGACCGA CTCCGTGCGG TCTGGCCGGA 
TGGCCAGGAG TTTACACGCT GGTGGGCAAG TACGTTGATT GGATACAAAA CACTATTGAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262597.1
CDS	695..1480
Translation	MKTKIDEFPWMALIEYTKSQGKKGHHCGGSLISTRYVITASHCVNGKALPTDWRLSGVRLGEWDTNTNPDCEVDVRGMKDCAPPHLDVPVERTIPHPDYIPASKNQVNDIALLRLAQQVEYTDFVRPICLPLDVNLRSATFDGITMDVAGWGKTEQLSASNLKLKAAVEGFRMDECQNVYSSQDILLEDTQMCAGGKEGVDSCRGDSGGPLIGLDTNKVNTYYFLAGVVSFGPTPCGLAGWPGVYTLVGKYVDWIQNTIES

Target ORF information:

RefSeq Version	NM_001275668.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster easter (ea), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275668.1

ORF Insert Sequence:

ATGAAGACCA AGATCGACGA GTTCCCCTGG ATGGCTCTAA TTGAATACAC CAAAAGCCAG 
GGCAAAAAGG GTCACCATTG CGGCGGATCC TTGATCAGCA CAAGATATGT GATCACAGCC 
TCGCATTGTG TCAACGGAAA GGCACTGCCC ACCGACTGGC GGTTGTCCGG TGTTCGTTTG 
GGCGAATGGG ACACCAACAC TAATCCCGAT TGCGAAGTAG ATGTGCGCGG CATGAAGGAC 
TGCGCGCCAC CGCATCTGGA CGTGCCCGTG GAGCGTACTA TACCCCATCC CGACTATATA 
CCGGCCTCCA AGAACCAGGT CAACGACATA GCCCTGCTGC GTCTGGCCCA GCAGGTGGAG 
TACACCGACT TTGTGCGACC CATTTGCCTG CCTCTGGACG TCAATCTGCG GTCGGCCACC 
TTCGACGGCA TCACCATGGA CGTGGCCGGC TGGGGCAAAA CGGAGCAGCT GTCGGCCAGC 
AACCTCAAGC TGAAGGCAGC CGTGGAGGGA TTCCGGATGG ATGAGTGCCA GAACGTCTAC 
AGCAGCCAGG ACATTCTCCT CGAGGACACA CAGATGTGTG CCGGCGGCAA GGAAGGCGTC 
GACTCCTGTC GCGGTGACTC CGGGGGACCG CTCATCGGCT TGGACACCAA CAAGGTGAAC 
ACCTACTACT TCCTGGCCGG CGTCGTGTCA TTTGGACCGA CTCCGTGCGG TCTGGCCGGA 
TGGCCAGGAG TTTACACGCT GGTGGGCAAG TACGTTGATT GGATACAAAA CACTATTGAG 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ea cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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