ems cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ems
Entrez Gene ID	41697
Full Name	empty spiracles
Synonyms	CG2988,Dmel\CG2988,E4,EMS,Ems,W13,ant
General protein information	Preferred Names empty spiracles Names CG2988 gene product from transcript CG2988-RA CG2988-PA antenna empty spiracle empty-spiracle empty-spiracles ems-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88A2-88A2\|3-54.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_169560.2	NP_731868.1	empty spiracles

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression and function of empty spiracles gene in olfactory sense organ development.
Title: Expression and function of the empty spiracles gene in olfactory sense organ development of Drosophila melanogaster.

These results indicate that the anteroposterior patterning gene ems controls the expression of DV genes, and vice versa.
Title: Ems and Nkx6 are central regulators in dorsoventral patterning of the Drosophila brain.

Data show that Drosophila olfactory local interneurons and projection neurons derive from a common neuroblast lineage specified by the empty spiracles gene.
Title: Drosophila olfactory local interneurons and projection neurons derive from a common neuroblast lineage specified by the empty spiracles gene.

ems is required for the olfactory circuitry.
Title: Empty spiracles is required for the development of olfactory projection neuron circuitry in Drosophila.

Data show that ems gene is involved in the specification of the anterior brain during embryonic CNS development in Drosophila.
Title: Anteroposterior regionalization of the brain: genetic and comparative aspects.

The following ems gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ems cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08367	NM_169560.2 Latest version!	Drosophila melanogaster empty spiracles (ems), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ems ( NM_169560.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731868.1 Drosophila melanogaster empty spiracles (ems), mRNA.

Price & Availability↑

CloneID	OFa08367
Clone ID Related Accession (Same CDS sequence)	NM_169560.2
Accession Version	NM_169560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	empty spiracles
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169560.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTAAGA TGATTCCGCC GGTTCCCACC GCCGCTGCAG CCGTCATGAT GCCCACACCA 
AAGCAGAAGA TCGGTTTCAG CATCGAGTCC ATTGTGGGCA ATGATGTCAG CACCGCCGGC 
GGCAATTCGA CGCCCGATCT ATCCGGTCCC CAGAGTCCGC CGCCCGGCGA GCGCAATGTG 
CCCGGTTCGC CGCCCCAAAC GCCACCAGCC ACACTCACCT TGATCCCAGG CTCGCCCCCG 
CACCATCTGA TGGCTCCTCC TGCCCACGGA CTGCCCTATC CGCATCCGCA TGCTCAGCAG 
CAGCATTTGC AGGCACCACA TCCGCATCCG CATCTATCGC CTGCGCAGCA ACATGTGCTC 
CACCAGCACC TCCTCATGCA GCAGCAACAT CCGGGCACAC CCAAGAGCCA CCAGGACATT 
CAGGAGCTGC TGCAGAGGCT GCACCACAAT GCCGCCATGG CCAGCGGCCT GAGTCCGCTG 
CAGACACGCC TCTCCCCCGA AACGGAGCAG CCCCAGATGG CCGTCTCCCT CAAGCGGGAA 
CGCTCACCTG CACCACCTGC CATGGAGCAG GCCGAGAATC CCGCCCAGCG CATCCAGCCA 
CCGCACACGC CTCCCAAATC CGTGAGCCCC CAGTCCTCGC AGCCCTCGTC TTCGCCCACT 
CTGCTGATCA GCAGTCCACA TGCCACACCG CCACAGCAGC AACAGCAGCA GCCGCCCCCG 
AATTATCCAA AGCCCGCCAT GATGCATCCC GGAGGAGCTG GACCCATGAT GATGCCGGGC 
ATGCCGCCCG CTGGACTCGT TCGCCCCTTT CCCATGGGAC CCGGAGGACC ACCGATGCCG 
CAGGGTCAGC CCGGCTTGCC GGACATCAAG GCGCTGCCGC CCTACATCAA CGCTCCGCCG 
GAGCTGCCGC CTCAGCACAA TCCGCATCTC ATTGCCGCTG CCCAGTTCCA GATGGCCGCC 
GCCCTGCAAG CGGGTCATGT GCTTGGTCCA GCGGCCGCCG CCGCTGCTGC CGCCGGCCTG 
CCGCCCCATG CCGCCCAGTT TATGCCCAAT CCAGGCATGG CCAGGGATAG CTATCAGCTG 
TACCCCTGGC TGCTCAGCCG CCATGGAAGG ATCTTCCCAC ACCGATTCCC TGGAAGTTTC 
CTGGTGCCCC CGTTCCGCAA ACCGAAACGC ATCCGCACCG CCTTCTCGCC GTCGCAGCTG 
CTGAAGCTGG AGCACGCCTT TGAGAGCAAC CAGTACGTAG TGGGGGCAGA GAGAAAGGCC 
CTGGCCCAAA ACCTCAATCT GTCCGAGACG CAGGTGAAGG TGTGGTTCCA GAACCGACGC 
ACCAAGCACA AGCGGATGCA GCAGGAGGAT GAGAAGGGCG GCGAGGGAGG ATCGCAGCGG 
AATATGCACA ACGGCAGCGG CGACGAGGAC GACGACGAAC TGATCGACAT GGAGATGGAC 
GAGTGTCCCA GCGATGAGGA GCACGAGCTG GACGCCAGCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731868.1
CDS	327..1811
Translation	MTKMIPPVPTAAAAVMMPTPKQKIGFSIESIVGNDVSTAGGNSTPDLSGPQSPPPGERNVPGSPPQTPPATLTLIPGSPPHHLMAPPAHGLPYPHPHAQQQHLQAPHPHPHLSPAQQHVLHQHLLMQQQHPGTPKSHQDIQELLQRLHHNAAMASGLSPLQTRLSPETEQPQMAVSLKRERSPAPPAMEQAENPAQRIQPPHTPPKSVSPQSSQPSSSPTLLISSPHATPPQQQQQQPPPNYPKPAMMHPGGAGPMMMPGMPPAGLVRPFPMGPGGPPMPQGQPGLPDIKALPPYINAPPELPPQHNPHLIAAAQFQMAAALQAGHVLGPAAAAAAAAGLPPHAAQFMPNPGMARDSYQLYPWLLSRHGRIFPHRFPGSFLVPPFRKPKRIRTAFSPSQLLKLEHAFESNQYVVGAERKALAQNLNLSETQVKVWFQNRRTKHKRMQQEDEKGGEGGSQRNMHNGSGDEDDDELIDMEMDECPSDEEHELDASH

Target ORF information:

RefSeq Version	NM_169560.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster empty spiracles (ems), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169560.2

ORF Insert Sequence:

ATGACTAAGA TGATTCCGCC GGTTCCCACC GCCGCTGCAG CCGTCATGAT GCCCACACCA 
AAGCAGAAGA TCGGTTTCAG CATCGAGTCC ATTGTGGGCA ATGATGTCAG CACCGCCGGC 
GGCAATTCGA CGCCCGATCT ATCCGGTCCC CAGAGTCCGC CGCCCGGCGA GCGCAATGTG 
CCCGGTTCGC CGCCCCAAAC GCCACCAGCC ACACTCACCT TGATCCCAGG CTCGCCCCCG 
CACCATCTGA TGGCTCCTCC TGCCCACGGA CTGCCCTATC CGCATCCGCA TGCTCAGCAG 
CAGCATTTGC AGGCACCACA TCCGCATCCG CATCTATCGC CTGCGCAGCA ACATGTGCTC 
CACCAGCACC TCCTCATGCA GCAGCAACAT CCGGGCACAC CCAAGAGCCA CCAGGACATT 
CAGGAGCTGC TGCAGAGGCT GCACCACAAT GCCGCCATGG CCAGCGGCCT GAGTCCGCTG 
CAGACACGCC TCTCCCCCGA AACGGAGCAG CCCCAGATGG CCGTCTCCCT CAAGCGGGAA 
CGCTCACCTG CACCACCTGC CATGGAGCAG GCCGAGAATC CCGCCCAGCG CATCCAGCCA 
CCGCACACGC CTCCCAAATC CGTGAGCCCC CAGTCCTCGC AGCCCTCGTC TTCGCCCACT 
CTGCTGATCA GCAGTCCACA TGCCACACCG CCACAGCAGC AACAGCAGCA GCCGCCCCCG 
AATTATCCAA AGCCCGCCAT GATGCATCCC GGAGGAGCTG GACCCATGAT GATGCCGGGC 
ATGCCGCCCG CTGGACTCGT TCGCCCCTTT CCCATGGGAC CCGGAGGACC ACCGATGCCG 
CAGGGTCAGC CCGGCTTGCC GGACATCAAG GCGCTGCCGC CCTACATCAA CGCTCCGCCG 
GAGCTGCCGC CTCAGCACAA TCCGCATCTC ATTGCCGCTG CCCAGTTCCA GATGGCCGCC 
GCCCTGCAAG CGGGTCATGT GCTTGGTCCA GCGGCCGCCG CCGCTGCTGC CGCCGGCCTG 
CCGCCCCATG CCGCCCAGTT TATGCCCAAT CCAGGCATGG CCAGGGATAG CTATCAGCTG 
TACCCCTGGC TGCTCAGCCG CCATGGAAGG ATCTTCCCAC ACCGATTCCC TGGAAGTTTC 
CTGGTGCCCC CGTTCCGCAA ACCGAAACGC ATCCGCACCG CCTTCTCGCC GTCGCAGCTG 
CTGAAGCTGG AGCACGCCTT TGAGAGCAAC CAGTACGTAG TGGGGGCAGA GAGAAAGGCC 
CTGGCCCAAA ACCTCAATCT GTCCGAGACG CAGGTGAAGG TGTGGTTCCA GAACCGACGC 
ACCAAGCACA AGCGGATGCA GCAGGAGGAT GAGAAGGGCG GCGAGGGAGG ATCGCAGCGG 
AATATGCACA ACGGCAGCGG CGACGAGGAC GACGACGAAC TGATCGACAT GGAGATGGAC 
GAGTGTCCCA GCGATGAGGA GCACGAGCTG GACGCCAGCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ems cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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