Ace cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ace
Entrez Gene ID	41625
Full Name	Acetylcholine esterase
Synonyms	ACE,AChE,AcChE,AchE,CG17907,CHE,DmAChE,Dm_ace,Dmel\CG17907,FBgn0000024,ace,ace-2,ache,dAChE,dmAChE,l(3)26,l(3)87Ed
General protein information	Preferred Names Acetylcholine esterase Names CG17907 gene product from transcript CG17907-RC Ace-PA Ace-PB Ace-PC CG17907-PA CG17907-PB CG17907-PC acetyl choline esterase acetylcholine esterase acetylcholinesterase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87E2-87E3\|3-52.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057605.5	NP_476953.1	acetylcholine esterase, isoform A
NM_001275601.1	NP_001262530.1	acetylcholine esterase, isoform C
NM_001170129.2	NP_001163600.1	acetylcholine esterase, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000466	XP_310628.3
Drosophila melanogaster (fruit fly)	Ace	NP_001163600.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

molecular dynamics simulation reveals the insecticidal resistance mechanism of AChE and its mutation
Title: A butterfly effect: highly insecticidal resistance caused by only a conservative residue mutated of drosophila melanogaster acetylcholinesterase.

kinetic studies show that it affects interactions with peripheral ligands and the entrance of some of the bulkier substrates
Title: Evidence for subdomain flexibility in Drosophila melanogaster acetylcholinesterase.

Results provide insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge.
Title: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel.

1 engineered disulfide bridge, I327C/D375C increased stability to heat denaturation, urea, & organic solvents & provided protease resistance. The new disulfide bridge links the N-terminal domain (first 356 aa) to the C-terminal domain.
Title: The effect of engineered disulfide bonds on the stability of Drosophila melanogaster acetylcholinesterase.

The following Ace gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ace cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04776	NM_057605.5 Latest version!	Drosophila melanogaster acetylcholine esterase, transcript variant A (Ace), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04776	NM_001170129.2 Latest version!	Drosophila melanogaster acetylcholine esterase, transcript variant B (Ace), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07721	NM_001275601.1 Latest version!	Drosophila melanogaster acetylcholine esterase, transcript variant C (Ace), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ace ( NM_057605.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476953.1 Drosophila melanogaster acetylcholine esterase, transcript variant A (Ace), mRNA.

Price & Availability↑

CloneID	OFa04776
Clone ID Related Accession (Same CDS sequence)	NM_057605.5 , NM_001170129.2
Accession Version	NM_057605.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	acetylcholine esterase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057605.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCT CCTGTCGGCA GAGCAGAGTC CTGCCCATGT CCTTGCCCCT GCCTCTGACC 
ATCCCGCTGC CCCTGGTGCT GGTGCTATCA CTGCACCTGT CCGGCGTCTG CGGCGTCATC 
GATCGCCTGG TCGTGCAGAC ATCCTCCGGA CCTGTACGCG GTCGCTCCGT GACGGTGCAG 
GGCAGGGAGG TGCATGTCTA CACGGGCATC CCCTACGCCA AGCCGCCCGT CGAGGACCTG 
CGCTTCCGAA AGCCGGTTCC CGCGGAGCCA TGGCACGGCG TCCTCGACGC CACGCGGTTA 
TCCGCCACCT GCGTCCAAGA GCGTTACGAG TACTTCCCCG GCTTCTCCGG CGAGGAGATC 
TGGAACCCCA ACACCAACGT GTCCGAGGAC TGCCTCTACA TAAATGTCTG GGCGCCGGCA 
AAGGCCCGAC TTCGCCATGG GCGGGGTGCC AACGGGGGTG AGCACCCCAA TGGCAAACAG 
GCGGACACTG ACCATCTCAT CCACAACGGA AATCCGCAGA ACACGACCAA CGGACTGCCG 
ATTCTGATCT GGATCTATGG CGGTGGCTTC ATGACCGGAT CGGCCACCCT GGACATCTAC 
AATGCGGATA TCATGGCCGC CGTGGGCAAT GTAATAGTGG CCTCCTTCCA GTATCGGGTG 
GGAGCCTTTG GGTTCTTGCA CCTGGCGCCG GAAATGCCGT CGGAATTCGC GGAAGAGGCG 
CCCGGCAATG TGGGCCTATG GGATCAGGCA CTCGCCATTC GCTGGCTGAA GGACAACGCT 
CATGCCTTCG GCGGAAATCC GGAGTGGATG ACACTGTTCG GAGAGTCGGC TGGATCCAGT 
TCGGTGAATG CCCAGCTCAT GTCGCCGGTG ACGAGGGGTC TGGTCAAGCG CGGAATGATG 
CAGTCGGGCA CTATGAACGC CCCCTGGAGC CACATGACCT CCGAGAAGGC CGTGGAGATC 
GGCAAGGCGC TGATCAACGA CTGCAACTGC AATGCATCTA TGCTGAAGAC CAATCCCGCT 
CACGTGATGA GCTGCATGCG TTCCGTGGAC GCCAAGACCA TATCGGTGCA GCAGTGGAAC 
TCCTACTCGG GCATCCTCAG CTTTCCCTCG GCGCCCACCA TTGATGGTGC GTTCCTGCCG 
GCGGATCCCA TGACGCTGAT GAAGACGGCG GATCTGAAGG ACTACGACAT CCTGATGGGA 
AATGTCAGGG ATGAGGGCAC TTACTTCTTG CTGTACGATT TCATCGATTA CTTCGATAAG 
GACGATGCCA CGGCCCTGCC ACGGGACAAA TACCTGGAAA TTATGAACAA TATTTTTGGC 
AAGGCAACGC AAGCGGAACG CGAGGCCATC ATTTTCCAGT ATACCAGTTG GGAAGGCAAT 
CCTGGCTATC AGAACCAGCA GCAAATCGGA CGCGCCGTGG GCGATCACTT CTTCACCTGC 
CCCACCAACG AGTATGCCCA GGCTCTGGCG GAGCGAGGCG CTTCCGTGCA CTACTACTAC 
TTTACACACC GCACAAGCAC CTCATTGTGG GGCGAATGGA TGGGCGTGCT GCACGGCGAT 
GAGATCGAAT ACTTCTTTGG CCAGCCGCTG AACAACTCCC TGCAGTATCG ACCTGTGGAG 
CGTGAGCTGG GCAAGCGTAT GCTCAGTGCG GTCATCGAGT TTGCTAAGAC GGGAAATCCC 
GCTCAGGATG GCGAGGAGTG GCCCAACTTC TCCAAGGAGG ATCCCGTCTA CTATATTTTC 
AGCACCGACG ATAAGATCGA GAAATTGGCC AGGGGTCCTT TGGCGGCTCG CTGCTCGTTC 
TGGAATGATT ACTTGCCAAA AGTCAGGAGT TGGGCAGGTA CTTGCGATGG CGATTCGGGA 
AGTGCTTCCA TATCCCCGAG GCTCCAGCTC CTTGGAATCG CTGCTCTGAT CTACATCTGC 
GCCGCATTGC GAACCAAAAG GGTTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476953.1
CDS	998..2947
Translation	MAISCRQSRVLPMSLPLPLTIPLPLVLVLSLHLSGVCGVIDRLVVQTSSGPVRGRSVTVQGREVHVYTGIPYAKPPVEDLRFRKPVPAEPWHGVLDATRLSATCVQERYEYFPGFSGEEIWNPNTNVSEDCLYINVWAPAKARLRHGRGANGGEHPNGKQADTDHLIHNGNPQNTTNGLPILIWIYGGGFMTGSATLDIYNADIMAAVGNVIVASFQYRVGAFGFLHLAPEMPSEFAEEAPGNVGLWDQALAIRWLKDNAHAFGGNPEWMTLFGESAGSSSVNAQLMSPVTRGLVKRGMMQSGTMNAPWSHMTSEKAVEIGKALINDCNCNASMLKTNPAHVMSCMRSVDAKTISVQQWNSYSGILSFPSAPTIDGAFLPADPMTLMKTADLKDYDILMGNVRDEGTYFLLYDFIDYFDKDDATALPRDKYLEIMNNIFGKATQAEREAIIFQYTSWEGNPGYQNQQQIGRAVGDHFFTCPTNEYAQALAERGASVHYYYFTHRTSTSLWGEWMGVLHGDEIEYFFGQPLNNSLQYRPVERELGKRMLSAVIEFAKTGNPAQDGEEWPNFSKEDPVYYIFSTDDKIEKLARGPLAARCSFWNDYLPKVRSWAGTCDGDSGSASISPRLQLLGIAALIYICAALRTKRVF

Target ORF information:

RefSeq Version	NM_057605.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster acetylcholine esterase, transcript variant A (Ace), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057605.5

ORF Insert Sequence:

ATGGCCATCT CCTGTCGGCA GAGCAGAGTC CTGCCCATGT CCTTGCCCCT GCCTCTGACC 
ATCCCGCTGC CCCTGGTGCT GGTGCTATCA CTGCACCTGT CCGGCGTCTG CGGCGTCATC 
GATCGCCTGG TCGTGCAGAC ATCCTCCGGA CCTGTACGCG GTCGCTCCGT GACGGTGCAG 
GGCAGGGAGG TGCATGTCTA CACGGGCATC CCCTACGCCA AGCCGCCCGT CGAGGACCTG 
CGCTTCCGAA AGCCGGTTCC CGCGGAGCCA TGGCACGGCG TCCTCGACGC CACGCGGTTA 
TCCGCCACCT GCGTCCAAGA GCGTTACGAG TACTTCCCCG GCTTCTCCGG CGAGGAGATC 
TGGAACCCCA ACACCAACGT GTCCGAGGAC TGCCTCTACA TAAATGTCTG GGCGCCGGCA 
AAGGCCCGAC TTCGCCATGG GCGGGGTGCC AACGGGGGTG AGCACCCCAA TGGCAAACAG 
GCGGACACTG ACCATCTCAT CCACAACGGA AATCCGCAGA ACACGACCAA CGGACTGCCG 
ATTCTGATCT GGATCTATGG CGGTGGCTTC ATGACCGGAT CGGCCACCCT GGACATCTAC 
AATGCGGATA TCATGGCCGC CGTGGGCAAT GTAATAGTGG CCTCCTTCCA GTATCGGGTG 
GGAGCCTTTG GGTTCTTGCA CCTGGCGCCG GAAATGCCGT CGGAATTCGC GGAAGAGGCG 
CCCGGCAATG TGGGCCTATG GGATCAGGCA CTCGCCATTC GCTGGCTGAA GGACAACGCT 
CATGCCTTCG GCGGAAATCC GGAGTGGATG ACACTGTTCG GAGAGTCGGC TGGATCCAGT 
TCGGTGAATG CCCAGCTCAT GTCGCCGGTG ACGAGGGGTC TGGTCAAGCG CGGAATGATG 
CAGTCGGGCA CTATGAACGC CCCCTGGAGC CACATGACCT CCGAGAAGGC CGTGGAGATC 
GGCAAGGCGC TGATCAACGA CTGCAACTGC AATGCATCTA TGCTGAAGAC CAATCCCGCT 
CACGTGATGA GCTGCATGCG TTCCGTGGAC GCCAAGACCA TATCGGTGCA GCAGTGGAAC 
TCCTACTCGG GCATCCTCAG CTTTCCCTCG GCGCCCACCA TTGATGGTGC GTTCCTGCCG 
GCGGATCCCA TGACGCTGAT GAAGACGGCG GATCTGAAGG ACTACGACAT CCTGATGGGA 
AATGTCAGGG ATGAGGGCAC TTACTTCTTG CTGTACGATT TCATCGATTA CTTCGATAAG 
GACGATGCCA CGGCCCTGCC ACGGGACAAA TACCTGGAAA TTATGAACAA TATTTTTGGC 
AAGGCAACGC AAGCGGAACG CGAGGCCATC ATTTTCCAGT ATACCAGTTG GGAAGGCAAT 
CCTGGCTATC AGAACCAGCA GCAAATCGGA CGCGCCGTGG GCGATCACTT CTTCACCTGC 
CCCACCAACG AGTATGCCCA GGCTCTGGCG GAGCGAGGCG CTTCCGTGCA CTACTACTAC 
TTTACACACC GCACAAGCAC CTCATTGTGG GGCGAATGGA TGGGCGTGCT GCACGGCGAT 
GAGATCGAAT ACTTCTTTGG CCAGCCGCTG AACAACTCCC TGCAGTATCG ACCTGTGGAG 
CGTGAGCTGG GCAAGCGTAT GCTCAGTGCG GTCATCGAGT TTGCTAAGAC GGGAAATCCC 
GCTCAGGATG GCGAGGAGTG GCCCAACTTC TCCAAGGAGG ATCCCGTCTA CTATATTTTC 
AGCACCGACG ATAAGATCGA GAAATTGGCC AGGGGTCCTT TGGCGGCTCG CTGCTCGTTC 
TGGAATGATT ACTTGCCAAA AGTCAGGAGT TGGGCAGGTA CTTGCGATGG CGATTCGGGA 
AGTGCTTCCA TATCCCCGAG GCTCCAGCTC CTTGGAATCG CTGCTCTGAT CTACATCTGC 
GCCGCATTGC GAACCAAAAG GGTTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ace ( NM_001170129.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163600.1 Drosophila melanogaster acetylcholine esterase, transcript variant B (Ace), mRNA.

Price & Availability↑

CloneID	OFa04776
Clone ID Related Accession (Same CDS sequence)	NM_057605.5 , NM_001170129.2
Accession Version	NM_001170129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	acetylcholine esterase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170129.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCT CCTGTCGGCA GAGCAGAGTC CTGCCCATGT CCTTGCCCCT GCCTCTGACC 
ATCCCGCTGC CCCTGGTGCT GGTGCTATCA CTGCACCTGT CCGGCGTCTG CGGCGTCATC 
GATCGCCTGG TCGTGCAGAC ATCCTCCGGA CCTGTACGCG GTCGCTCCGT GACGGTGCAG 
GGCAGGGAGG TGCATGTCTA CACGGGCATC CCCTACGCCA AGCCGCCCGT CGAGGACCTG 
CGCTTCCGAA AGCCGGTTCC CGCGGAGCCA TGGCACGGCG TCCTCGACGC CACGCGGTTA 
TCCGCCACCT GCGTCCAAGA GCGTTACGAG TACTTCCCCG GCTTCTCCGG CGAGGAGATC 
TGGAACCCCA ACACCAACGT GTCCGAGGAC TGCCTCTACA TAAATGTCTG GGCGCCGGCA 
AAGGCCCGAC TTCGCCATGG GCGGGGTGCC AACGGGGGTG AGCACCCCAA TGGCAAACAG 
GCGGACACTG ACCATCTCAT CCACAACGGA AATCCGCAGA ACACGACCAA CGGACTGCCG 
ATTCTGATCT GGATCTATGG CGGTGGCTTC ATGACCGGAT CGGCCACCCT GGACATCTAC 
AATGCGGATA TCATGGCCGC CGTGGGCAAT GTAATAGTGG CCTCCTTCCA GTATCGGGTG 
GGAGCCTTTG GGTTCTTGCA CCTGGCGCCG GAAATGCCGT CGGAATTCGC GGAAGAGGCG 
CCCGGCAATG TGGGCCTATG GGATCAGGCA CTCGCCATTC GCTGGCTGAA GGACAACGCT 
CATGCCTTCG GCGGAAATCC GGAGTGGATG ACACTGTTCG GAGAGTCGGC TGGATCCAGT 
TCGGTGAATG CCCAGCTCAT GTCGCCGGTG ACGAGGGGTC TGGTCAAGCG CGGAATGATG 
CAGTCGGGCA CTATGAACGC CCCCTGGAGC CACATGACCT CCGAGAAGGC CGTGGAGATC 
GGCAAGGCGC TGATCAACGA CTGCAACTGC AATGCATCTA TGCTGAAGAC CAATCCCGCT 
CACGTGATGA GCTGCATGCG TTCCGTGGAC GCCAAGACCA TATCGGTGCA GCAGTGGAAC 
TCCTACTCGG GCATCCTCAG CTTTCCCTCG GCGCCCACCA TTGATGGTGC GTTCCTGCCG 
GCGGATCCCA TGACGCTGAT GAAGACGGCG GATCTGAAGG ACTACGACAT CCTGATGGGA 
AATGTCAGGG ATGAGGGCAC TTACTTCTTG CTGTACGATT TCATCGATTA CTTCGATAAG 
GACGATGCCA CGGCCCTGCC ACGGGACAAA TACCTGGAAA TTATGAACAA TATTTTTGGC 
AAGGCAACGC AAGCGGAACG CGAGGCCATC ATTTTCCAGT ATACCAGTTG GGAAGGCAAT 
CCTGGCTATC AGAACCAGCA GCAAATCGGA CGCGCCGTGG GCGATCACTT CTTCACCTGC 
CCCACCAACG AGTATGCCCA GGCTCTGGCG GAGCGAGGCG CTTCCGTGCA CTACTACTAC 
TTTACACACC GCACAAGCAC CTCATTGTGG GGCGAATGGA TGGGCGTGCT GCACGGCGAT 
GAGATCGAAT ACTTCTTTGG CCAGCCGCTG AACAACTCCC TGCAGTATCG ACCTGTGGAG 
CGTGAGCTGG GCAAGCGTAT GCTCAGTGCG GTCATCGAGT TTGCTAAGAC GGGAAATCCC 
GCTCAGGATG GCGAGGAGTG GCCCAACTTC TCCAAGGAGG ATCCCGTCTA CTATATTTTC 
AGCACCGACG ATAAGATCGA GAAATTGGCC AGGGGTCCTT TGGCGGCTCG CTGCTCGTTC 
TGGAATGATT ACTTGCCAAA AGTCAGGAGT TGGGCAGGTA CTTGCGATGG CGATTCGGGA 
AGTGCTTCCA TATCCCCGAG GCTCCAGCTC CTTGGAATCG CTGCTCTGAT CTACATCTGC 
GCCGCATTGC GAACCAAAAG GGTTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163600.1
CDS	711..2660
Translation	MAISCRQSRVLPMSLPLPLTIPLPLVLVLSLHLSGVCGVIDRLVVQTSSGPVRGRSVTVQGREVHVYTGIPYAKPPVEDLRFRKPVPAEPWHGVLDATRLSATCVQERYEYFPGFSGEEIWNPNTNVSEDCLYINVWAPAKARLRHGRGANGGEHPNGKQADTDHLIHNGNPQNTTNGLPILIWIYGGGFMTGSATLDIYNADIMAAVGNVIVASFQYRVGAFGFLHLAPEMPSEFAEEAPGNVGLWDQALAIRWLKDNAHAFGGNPEWMTLFGESAGSSSVNAQLMSPVTRGLVKRGMMQSGTMNAPWSHMTSEKAVEIGKALINDCNCNASMLKTNPAHVMSCMRSVDAKTISVQQWNSYSGILSFPSAPTIDGAFLPADPMTLMKTADLKDYDILMGNVRDEGTYFLLYDFIDYFDKDDATALPRDKYLEIMNNIFGKATQAEREAIIFQYTSWEGNPGYQNQQQIGRAVGDHFFTCPTNEYAQALAERGASVHYYYFTHRTSTSLWGEWMGVLHGDEIEYFFGQPLNNSLQYRPVERELGKRMLSAVIEFAKTGNPAQDGEEWPNFSKEDPVYYIFSTDDKIEKLARGPLAARCSFWNDYLPKVRSWAGTCDGDSGSASISPRLQLLGIAALIYICAALRTKRVF

Target ORF information:

RefSeq Version	NM_001170129.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster acetylcholine esterase, transcript variant B (Ace), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170129.2

ORF Insert Sequence:

ATGGCCATCT CCTGTCGGCA GAGCAGAGTC CTGCCCATGT CCTTGCCCCT GCCTCTGACC 
ATCCCGCTGC CCCTGGTGCT GGTGCTATCA CTGCACCTGT CCGGCGTCTG CGGCGTCATC 
GATCGCCTGG TCGTGCAGAC ATCCTCCGGA CCTGTACGCG GTCGCTCCGT GACGGTGCAG 
GGCAGGGAGG TGCATGTCTA CACGGGCATC CCCTACGCCA AGCCGCCCGT CGAGGACCTG 
CGCTTCCGAA AGCCGGTTCC CGCGGAGCCA TGGCACGGCG TCCTCGACGC CACGCGGTTA 
TCCGCCACCT GCGTCCAAGA GCGTTACGAG TACTTCCCCG GCTTCTCCGG CGAGGAGATC 
TGGAACCCCA ACACCAACGT GTCCGAGGAC TGCCTCTACA TAAATGTCTG GGCGCCGGCA 
AAGGCCCGAC TTCGCCATGG GCGGGGTGCC AACGGGGGTG AGCACCCCAA TGGCAAACAG 
GCGGACACTG ACCATCTCAT CCACAACGGA AATCCGCAGA ACACGACCAA CGGACTGCCG 
ATTCTGATCT GGATCTATGG CGGTGGCTTC ATGACCGGAT CGGCCACCCT GGACATCTAC 
AATGCGGATA TCATGGCCGC CGTGGGCAAT GTAATAGTGG CCTCCTTCCA GTATCGGGTG 
GGAGCCTTTG GGTTCTTGCA CCTGGCGCCG GAAATGCCGT CGGAATTCGC GGAAGAGGCG 
CCCGGCAATG TGGGCCTATG GGATCAGGCA CTCGCCATTC GCTGGCTGAA GGACAACGCT 
CATGCCTTCG GCGGAAATCC GGAGTGGATG ACACTGTTCG GAGAGTCGGC TGGATCCAGT 
TCGGTGAATG CCCAGCTCAT GTCGCCGGTG ACGAGGGGTC TGGTCAAGCG CGGAATGATG 
CAGTCGGGCA CTATGAACGC CCCCTGGAGC CACATGACCT CCGAGAAGGC CGTGGAGATC 
GGCAAGGCGC TGATCAACGA CTGCAACTGC AATGCATCTA TGCTGAAGAC CAATCCCGCT 
CACGTGATGA GCTGCATGCG TTCCGTGGAC GCCAAGACCA TATCGGTGCA GCAGTGGAAC 
TCCTACTCGG GCATCCTCAG CTTTCCCTCG GCGCCCACCA TTGATGGTGC GTTCCTGCCG 
GCGGATCCCA TGACGCTGAT GAAGACGGCG GATCTGAAGG ACTACGACAT CCTGATGGGA 
AATGTCAGGG ATGAGGGCAC TTACTTCTTG CTGTACGATT TCATCGATTA CTTCGATAAG 
GACGATGCCA CGGCCCTGCC ACGGGACAAA TACCTGGAAA TTATGAACAA TATTTTTGGC 
AAGGCAACGC AAGCGGAACG CGAGGCCATC ATTTTCCAGT ATACCAGTTG GGAAGGCAAT 
CCTGGCTATC AGAACCAGCA GCAAATCGGA CGCGCCGTGG GCGATCACTT CTTCACCTGC 
CCCACCAACG AGTATGCCCA GGCTCTGGCG GAGCGAGGCG CTTCCGTGCA CTACTACTAC 
TTTACACACC GCACAAGCAC CTCATTGTGG GGCGAATGGA TGGGCGTGCT GCACGGCGAT 
GAGATCGAAT ACTTCTTTGG CCAGCCGCTG AACAACTCCC TGCAGTATCG ACCTGTGGAG 
CGTGAGCTGG GCAAGCGTAT GCTCAGTGCG GTCATCGAGT TTGCTAAGAC GGGAAATCCC 
GCTCAGGATG GCGAGGAGTG GCCCAACTTC TCCAAGGAGG ATCCCGTCTA CTATATTTTC 
AGCACCGACG ATAAGATCGA GAAATTGGCC AGGGGTCCTT TGGCGGCTCG CTGCTCGTTC 
TGGAATGATT ACTTGCCAAA AGTCAGGAGT TGGGCAGGTA CTTGCGATGG CGATTCGGGA 
AGTGCTTCCA TATCCCCGAG GCTCCAGCTC CTTGGAATCG CTGCTCTGAT CTACATCTGC 
GCCGCATTGC GAACCAAAAG GGTTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ace ( NM_001275601.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262530.1 Drosophila melanogaster acetylcholine esterase, transcript variant C (Ace), mRNA.

Price & Availability↑

CloneID	OFa07721
Clone ID Related Accession (Same CDS sequence)	NM_001275601.1
Accession Version	NM_001275601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	acetylcholine esterase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATCT CCTGTCGGCA GAGCAGAGTC CTGCCCATGT CCTTGCCCCT GCCTCTGACC 
ATCCCGCTGC CCCTGGTGCT GGTGCTATCA CTGCACCTGT CCGGCGTCTG CGGCGTCATC 
GATCGCCTGG TCGTGCAGAC ATCCTCCGGA CCTGTACGCG GTCGCTCCGT GACGGTGCAG 
GGCAGGGAGG TGCATGTCTA CACGGGCATC CCCTACGCCA AGCCGCCCGT CGAGGACCTG 
CGCTTCCGAA AGCCGGTTCC CGCGGAGCCA TGGCACGGCG TCCTCGACGC CACGCGGTTA 
TCCGCCACCT GCGTCCAAGA GCGTTACGAG TACTTCCCCG GCTTCTCCGG CGAGGAGATC 
TGGAACCCCA ACACCAACGT GTCCGAGGAC TGCCTCTACA TAAATGTCTG GGCGCCGGCA 
AAGGCCCGAC TTCGCCATGG GCGGGGTGCC AACGGGGGTG AGCACCCCAA TGGCAAACAG 
GCGGACACTG ACCATCTCAT CCACAACGGA AATCCGCAGA ACACGACCAA CGGACTGCCG 
ATTCTGATCT GGATCTATGG CGGTGGCTTC ATGACCGGAT CGGCCACCCT GGACATCTAC 
AATGCGGATA TCATGGCCGC CGTGGGCAAT GTAATAGTGG CCTCCTTCCA GTATCGGGTG 
GGAGCCTTTG GGTTCTTGCA CCTGGCGCCG GAAATGCCGT CGGAATTCGC GGAAGAGGCG 
CCCGGCAATG TGGGCCTATG GGATCAGGCA CTCGCCATTC GCTGGCTGAA GGACAACGCT 
CATGCCTTCG GCGGAAATCC GGAGTGGATG ACACTGTTCG GAGAGTCGGC TGGATCCAGT 
TCGGTGAATG CCCAGCTCAT GTCGCCGGTG ACGAGGGGTC TGGTCAAGCG CGGAATGATG 
CAGTCGGGCA CTATGAACGC CCCCTGGAGC CACATGACCT CCGAGAAGGC CGTGGAGATC 
GGCAAGGCGC TGATCAACGA CTGCAACTGC AATGCATCTA TGCTGAAGAC CAATCCCGCT 
CACGTGATGA GCTGCATGCG TTCCGTGGAC GCCAAGACCA TATCGGTGCA GCAGTGGAAC 
TCCTACTCGG GCATCCTCAG CTTTCCCTCG GCGCCCACCA TTGATGGTGC GTTCCTGCCG 
GCGGATCCCA TGACGCTGAT GAAGACGGCG GATCTGAAGG ACTACGACAT CCTGATGGGA 
AATGTCAGGG ATGAGGGCAC TTACTTCTTG CTGTACGATT TCATCGATTA CTTCGATAAG 
GACGATGCCA CGGCCCTGCC ACGGGACAAA TACCTGGAAA TTATGAACAA TATTTTTGGC 
AAGGCAACGC AAGCGGAACG CGAGGCCATC ATTTTCCAGT ATACCAGTTG GGAAGGCAAT 
CCTGGCTATC AGAACCAGCA GCAAATCGGA CGCGCCGTGG GCGATCACTT CTTCACCTGC 
CCCACCAACG AGTATGCCCA GGCTCTGGCG GAGCGAGGCG CTTCCGTGCA CTACTACTAC 
TTTACACACC GCACAAGCAC CTCATTGTGG GGCGAATGGA TGGGCGTGCT GCACGGCGAT 
GAGATCGAAT ACTTCTTTGG CCAGCCGCTG AACAACTCCC TGCAGTATCG ACCTGTGGAG 
CGTGAGCTGG GCAAGCGTAT GCTCAGTGCG GTCATCGAGT TTGCTAAGAC GGGAAATCCC 
GCTCAGGATG GCGAGGAGTG GCCCAACTTC TCCAAGGAGG ATCCCGTCTA CTATATTTTC 
AGCACCGACG ATAAGATCGA GAAATTGGCC AGGGGTCCTT TGGCGGCTCG CTGCTCGTTC 
TGGAATGATT ACTTGCCAAA AGTCAGGAGT TGGGCAGAAT TTGCGCAGCA CATAACCCAT 
GTGGCCACCT CGGTGTTTGT CTACCTGAAG ACCTGGCTAA TTAGTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262530.1
CDS	998..2908
Translation	MAISCRQSRVLPMSLPLPLTIPLPLVLVLSLHLSGVCGVIDRLVVQTSSGPVRGRSVTVQGREVHVYTGIPYAKPPVEDLRFRKPVPAEPWHGVLDATRLSATCVQERYEYFPGFSGEEIWNPNTNVSEDCLYINVWAPAKARLRHGRGANGGEHPNGKQADTDHLIHNGNPQNTTNGLPILIWIYGGGFMTGSATLDIYNADIMAAVGNVIVASFQYRVGAFGFLHLAPEMPSEFAEEAPGNVGLWDQALAIRWLKDNAHAFGGNPEWMTLFGESAGSSSVNAQLMSPVTRGLVKRGMMQSGTMNAPWSHMTSEKAVEIGKALINDCNCNASMLKTNPAHVMSCMRSVDAKTISVQQWNSYSGILSFPSAPTIDGAFLPADPMTLMKTADLKDYDILMGNVRDEGTYFLLYDFIDYFDKDDATALPRDKYLEIMNNIFGKATQAEREAIIFQYTSWEGNPGYQNQQQIGRAVGDHFFTCPTNEYAQALAERGASVHYYYFTHRTSTSLWGEWMGVLHGDEIEYFFGQPLNNSLQYRPVERELGKRMLSAVIEFAKTGNPAQDGEEWPNFSKEDPVYYIFSTDDKIEKLARGPLAARCSFWNDYLPKVRSWAEFAQHITHVATSVFVYLKTWLISG

Target ORF information:

RefSeq Version	NM_001275601.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster acetylcholine esterase, transcript variant C (Ace), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275601.1

ORF Insert Sequence:

ATGGCCATCT CCTGTCGGCA GAGCAGAGTC CTGCCCATGT CCTTGCCCCT GCCTCTGACC 
ATCCCGCTGC CCCTGGTGCT GGTGCTATCA CTGCACCTGT CCGGCGTCTG CGGCGTCATC 
GATCGCCTGG TCGTGCAGAC ATCCTCCGGA CCTGTACGCG GTCGCTCCGT GACGGTGCAG 
GGCAGGGAGG TGCATGTCTA CACGGGCATC CCCTACGCCA AGCCGCCCGT CGAGGACCTG 
CGCTTCCGAA AGCCGGTTCC CGCGGAGCCA TGGCACGGCG TCCTCGACGC CACGCGGTTA 
TCCGCCACCT GCGTCCAAGA GCGTTACGAG TACTTCCCCG GCTTCTCCGG CGAGGAGATC 
TGGAACCCCA ACACCAACGT GTCCGAGGAC TGCCTCTACA TAAATGTCTG GGCGCCGGCA 
AAGGCCCGAC TTCGCCATGG GCGGGGTGCC AACGGGGGTG AGCACCCCAA TGGCAAACAG 
GCGGACACTG ACCATCTCAT CCACAACGGA AATCCGCAGA ACACGACCAA CGGACTGCCG 
ATTCTGATCT GGATCTATGG CGGTGGCTTC ATGACCGGAT CGGCCACCCT GGACATCTAC 
AATGCGGATA TCATGGCCGC CGTGGGCAAT GTAATAGTGG CCTCCTTCCA GTATCGGGTG 
GGAGCCTTTG GGTTCTTGCA CCTGGCGCCG GAAATGCCGT CGGAATTCGC GGAAGAGGCG 
CCCGGCAATG TGGGCCTATG GGATCAGGCA CTCGCCATTC GCTGGCTGAA GGACAACGCT 
CATGCCTTCG GCGGAAATCC GGAGTGGATG ACACTGTTCG GAGAGTCGGC TGGATCCAGT 
TCGGTGAATG CCCAGCTCAT GTCGCCGGTG ACGAGGGGTC TGGTCAAGCG CGGAATGATG 
CAGTCGGGCA CTATGAACGC CCCCTGGAGC CACATGACCT CCGAGAAGGC CGTGGAGATC 
GGCAAGGCGC TGATCAACGA CTGCAACTGC AATGCATCTA TGCTGAAGAC CAATCCCGCT 
CACGTGATGA GCTGCATGCG TTCCGTGGAC GCCAAGACCA TATCGGTGCA GCAGTGGAAC 
TCCTACTCGG GCATCCTCAG CTTTCCCTCG GCGCCCACCA TTGATGGTGC GTTCCTGCCG 
GCGGATCCCA TGACGCTGAT GAAGACGGCG GATCTGAAGG ACTACGACAT CCTGATGGGA 
AATGTCAGGG ATGAGGGCAC TTACTTCTTG CTGTACGATT TCATCGATTA CTTCGATAAG 
GACGATGCCA CGGCCCTGCC ACGGGACAAA TACCTGGAAA TTATGAACAA TATTTTTGGC 
AAGGCAACGC AAGCGGAACG CGAGGCCATC ATTTTCCAGT ATACCAGTTG GGAAGGCAAT 
CCTGGCTATC AGAACCAGCA GCAAATCGGA CGCGCCGTGG GCGATCACTT CTTCACCTGC 
CCCACCAACG AGTATGCCCA GGCTCTGGCG GAGCGAGGCG CTTCCGTGCA CTACTACTAC 
TTTACACACC GCACAAGCAC CTCATTGTGG GGCGAATGGA TGGGCGTGCT GCACGGCGAT 
GAGATCGAAT ACTTCTTTGG CCAGCCGCTG AACAACTCCC TGCAGTATCG ACCTGTGGAG 
CGTGAGCTGG GCAAGCGTAT GCTCAGTGCG GTCATCGAGT TTGCTAAGAC GGGAAATCCC 
GCTCAGGATG GCGAGGAGTG GCCCAACTTC TCCAAGGAGG ATCCCGTCTA CTATATTTTC 
AGCACCGACG ATAAGATCGA GAAATTGGCC AGGGGTCCTT TGGCGGCTCG CTGCTCGTTC 
TGGAATGATT ACTTGCCAAA AGTCAGGAGT TGGGCAGAAT TTGCGCAGCA CATAACCCAT 
GTGGCCACCT CGGTGTTTGT CTACCTGAAG ACCTGGCTAA TTAGTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ace cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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