Cap-H2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cap-H2
Entrez Gene ID	41289
Full Name	Chromosome associated protein H2
Synonyms	CAP-H2,CG14685,CapH2,Dmel\CG14685,cap-H2,dCap-H2
General protein information	Preferred Names Chromosome associated protein H2 Names CG14685 gene product from transcript CG14685-RE CG14685-PD CG14685-PE CG14685-PF CG14685-PG Cap-H2-PD Cap-H2-PE Cap-H2-PF Cap-H2-PG chromosome associated protein H2 dCap-H2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C5-86C6\|3-49 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_169359.2	NP_731503.2	chromosome associated protein H2, isoform G
NM_169358.2	NP_731502.2	chromosome associated protein H2, isoform F
NM_206471.2	NP_996193.1	chromosome associated protein H2, isoform D
NM_141757.2	NP_650014.2	chromosome associated protein H2, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identify Casein Kinase I alpha (CK1alpha) as an additional negative-regulator of Cap-H2. CK1a-depletion stabilizes Cap-H2 protein and results in an accumulation of Cap-H2 on chromosomes.
Title: Drosophila casein kinase I alpha regulates homolog pairing and genome organization by modulating condensin II subunit Cap-H2 levels.

Cap-h2 interacts with Mrg15 and regulates interphase chromosome compaction.
Title: Maintenance of interphase chromosome compaction and homolog pairing in Drosophila is regulated by the condensin cap-h2 and its partner Mrg15.

Cap-H2 stabilization distorted nuclear envelopes and dispersed Cid/CENP-A on interphase chromosomes.
Title: SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2.

Cap-H2 is required for nurse cell chromosome territory formation
Title: Condensin II promotes the formation of chromosome territories by inducing axial compaction of polyploid interphase chromosomes.

findings show that condensin II - Cap-H2 promotes disassembly of polytene structure into chromosomal components; Condensin II also negatively regulates transvection
Title: Chromosome alignment and transvection are antagonized by condensin II.

The following Cap-H2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cap-H2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08281	NM_141757.2 Latest version!	Drosophila melanogaster chromosome associated protein H2, transcript variant E (Cap-H2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08282	NM_169359.2 Latest version!	Drosophila melanogaster chromosome associated protein H2, transcript variant G (Cap-H2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08283	NM_169358.2 Latest version!	Drosophila melanogaster chromosome associated protein H2, transcript variant F (Cap-H2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08284	NM_206471.2 Latest version!	Drosophila melanogaster chromosome associated protein H2, transcript variant D (Cap-H2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cap-H2 ( NM_141757.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650014.2 Drosophila melanogaster chromosome associated protein H2, transcript variant E (Cap-H2), mRNA.

Price & Availability↑

CloneID	OFa08281
Clone ID Related Accession (Same CDS sequence)	NM_141757.2
Accession Version	NM_141757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chromosome associated protein H2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_141757.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGGA TTTTGCCTGA AGAAGCGGAA GCGGCCTACT TGAGCGAAGC GCGGGAGCAG 
ATATTGGAAA TAGCCAAGAA TCGGCCGGGA ACCCAAGTGG CCAAGTGCAT CCGCGCCTAC 
GATGAGCAAC AGGACCTGGC CTCGCTAGTC GTCCTCCTCG AAGAGCTCTC CAAAAACACG 
GACTACTCCA CGGACCTACG GATATCACTG GGATATTATA TTGAAGAGCT ACTGCGCCAC 
TGCTTAGGAC GAAATGATGT CTCTCGAAGA TCGGCCATCG TGGCCGCAGG AAGTGCCCTC 
CAGTACTGCG GCAAGATCTA TGGAGACCGA GTGGAGTACC TGTGCCAGGT GGTGGAGCAC 
CAGATCGAAG CCCTGCTCAC ATCGGAGTTG CAGAAGGAGA CCCCTAGCGG AAGTGCTGCA 
CCGGAGAAAA ACGAGCGCAG GCCTGAGGAA ACGCGCAAAC GTCACCCTAA AAAGCTAACT 
AACAAGGAAG TTGATCCCTA TTTGCTCACG TTGGAACCGA AAAGGTTTAA AACCATGTCG 
GACGACAAGC GCTTCAACGC GGCGGGCTTT GTAAAGTGCA CGAGAAATCG GACGATAGAA 
TACCTTTATC AGGATCACAC GCCGCCCAAC CTGTGGAAAC ACGCGCCAAT TGTGGATCCT 
CACAATCCCT ACGACCAGGA TGAGAAAAAG CAATACAAAA TGTTTACGTA TCACGTAGAG 
CATAGATACA ATACGCTTCT GCCAGATATT CCCTTCGAAA GGCTGAATCT CATTAAAGAA 
TATGTTCACA CAAATCAGGT GAACACCACT GAGATTCTTA ATGAGCACAT GACCACAAAG 
GAATATCTGG ACGAGTATAT TGCGCTGGAG AATCAAATGC TAGCAGCCCG CTATGGAGCC 
ATTGTCACAA GGAGAAGAAG ATTGGTTGAT TCCGCGAGAT TTATGGAGGA CAATTTGGAT 
GAGGGACTGG CGAAAAAAAT GTGCATGGAC AAGAATCTGC CAATGGACAC GAACGAAACT 
GTTCTTATTG ACCAATCTTT GGTGGATGAA AACAGCAGGT CGTTAACCAC AGCCGAGTCT 
ACGATGGGCA TCAGTCAGGC GGAGAACTCT ACTTTAAAGA GTAGCGAAGT CGAGGCGACT 
TTAAGCGTCA GCCATATAGA AAACTCCTTA AGCGATAGCC AAGAAGAGAA TCCTCCGCTG 
AGCAGCACAT TAGCCATTAA CGAGTCTAGT GTTCTCGACA GCACGAGAGT GGATCCCATA 
AAAGATCTCA CTCTAGATGA ACTGTTGCTA ATCGATTCAG GGATCAGTAT GGAAGAGCTT 
TCCGATATTC AAATGCATAC AGCAGGCCAG TCTTTTGATG ATGAGGGTGT TGTTCTTTCA 
GATTTAGAAG ATCAACGCCA GTTGTCGCCC ATGTTGCAGA TGGTTAGCCC ATCGATGGAG 
GCAAAAACGC TCAATATCAT TGAGATGGAT GCTGATCTTA TCATGAATGT TCCAAGGGAG 
GTGAGCTATC CTATTCTGCT GAATGTTATG GGTCTCCCCA TAAAACGCCT GCGTCGGAAA 
TGCATCTTTA AACTTCCACC TGAGTTCGAC TTATTCAGGC AAGCACGCCT TCCTATTAAG 
CGAGAGGGAC AACAAAAATC CCCAACCACT CCCAGAACTG TACTGCAAAT TGGAAGAGAG 
GCTCCCCAAA ATGAAAGAGA ACCCGGCTCC CCCTGTAGTT TAGAATTTGA TGAGGACTTG 
AATTTTCTGG GTTTCCGCCA ACGTCGACCC ACATTTGACT CGGGCTTCGA TATCGAAGAG 
CCGCGTGTAT CAAGTTGTGT CTCCACTATC GGAGAAGTTA AAACTGAGCT TGAAGATGAG 
ATTGAAGCCA ACACCAATAA TTTAACTGAC GCGAGCGCCA GCGACGCCTT GAATGAGAAT 
GTGAACGATA CAAAGGAAAG TGGGCTGGGA GATTCACTAG CGCAGGAATT AAACGCTTCC 
ACCGAACCCA ATGCGGAAAA CAATACCGCA AATTCATCGC AACTTGAAGT GACTGCTACA 
GAATCCTCCG GAACGCTAAG CGGACTGGAA ACCACAGTGG ATTTAGGAAT GGAGAGCGCG 
TTAGTTAGTT CTGTATTGGA TTTAAGTACC GAACCGAGTG CAATGGATAG TAACATTATA 
AATGTCACCC AACCTGACAA TTCAGACCTA GAGATTTCAA CCGTGCAGGA CCAAGATACC 
TTACATGACG AAGCAGATGC AAATGATTTG TCTGCTGAAA CACCCAATCC AATTGCCGAC 
TCAGCAGTCG ACGACTGCTC ATTGATCCGG GACTGGCATA GACGATTGGC TCCTGCTTTG 
GAAGCAGCCC ATGAACGGCA GAATTTCAAT ATTAAGGACT TGGGCACCGA GATCCTGGAC 
ATTTGCAAGG CGGGCAACAG AACAGCCACG TTAGCCGACG TCATGGCTGA CAAGGATCCT 
AGTATCATGT GTCGCTACAT GCTGGCTTCC CTGGTACTGA CGAACCACGG AAACGTGTCT 
CTGGATTTTG AAAATCGCGA TAAAAGTAAG CCCATCGACA TGTCTCAGTT TCGAATGCAT 
TTAAAGAGTA TGAAGCGAAT GGAAATTAAT CCCGAGGACG ACGTGGGCAA CATAAATGCT 
GCCCAGAGCA AATCGACGCC GAGAAGTAAA CAGCTAAATG ATTCAGCTCA CAAGCCACGA 
ACAAGCACGA CGGCATCTGC ACCAACAAAA CGAAAGTCTG CGGAAAATTC TTTATCCGAG 
GTGTTTGCTA AAACTGTGCG ATTGATACAG CCCATCCCAA AGATGTGGCC TACTCCTTCT 
GATGCCGACT CGGGGATATC TTCGATGGGC TCATCATTGG CATCGACAGC CCGCCTGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650014.2
CDS	274..3156
Translation	MERILPEEAEAAYLSEAREQILEIAKNRPGTQVAKCIRAYDEQQDLASLVVLLEELSKNTDYSTDLRISLGYYIEELLRHCLGRNDVSRRSAIVAAGSALQYCGKIYGDRVEYLCQVVEHQIEALLTSELQKETPSGSAAPEKNERRPEETRKRHPKKLTNKEVDPYLLTLEPKRFKTMSDDKRFNAAGFVKCTRNRTIEYLYQDHTPPNLWKHAPIVDPHNPYDQDEKKQYKMFTYHVEHRYNTLLPDIPFERLNLIKEYVHTNQVNTTEILNEHMTTKEYLDEYIALENQMLAARYGAIVTRRRRLVDSARFMEDNLDEGLAKKMCMDKNLPMDTNETVLIDQSLVDENSRSLTTAESTMGISQAENSTLKSSEVEATLSVSHIENSLSDSQEENPPLSSTLAINESSVLDSTRVDPIKDLTLDELLLIDSGISMEELSDIQMHTAGQSFDDEGVVLSDLEDQRQLSPMLQMVSPSMEAKTLNIIEMDADLIMNVPREVSYPILLNVMGLPIKRLRRKCIFKLPPEFDLFRQARLPIKREGQQKSPTTPRTVLQIGREAPQNEREPGSPCSLEFDEDLNFLGFRQRRPTFDSGFDIEEPRVSSCVSTIGEVKTELEDEIEANTNNLTDASASDALNENVNDTKESGLGDSLAQELNASTEPNAENNTANSSQLEVTATESSGTLSGLETTVDLGMESALVSSVLDLSTEPSAMDSNIINVTQPDNSDLEISTVQDQDTLHDEADANDLSAETPNPIADSAVDDCSLIRDWHRRLAPALEAAHERQNFNIKDLGTEILDICKAGNRTATLADVMADKDPSIMCRYMLASLVLTNHGNVSLDFENRDKSKPIDMSQFRMHLKSMKRMEINPEDDVGNINAAQSKSTPRSKQLNDSAHKPRTSTTASAPTKRKSAENSLSEVFAKTVRLIQPIPKMWPTPSDADSGISSMGSSLASTARLK

Target ORF information:

RefSeq Version	NM_141757.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chromosome associated protein H2, transcript variant E (Cap-H2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141757.2

ORF Insert Sequence:

ATGGAGCGGA TTTTGCCTGA AGAAGCGGAA GCGGCCTACT TGAGCGAAGC GCGGGAGCAG 
ATATTGGAAA TAGCCAAGAA TCGGCCGGGA ACCCAAGTGG CCAAGTGCAT CCGCGCCTAC 
GATGAGCAAC AGGACCTGGC CTCGCTAGTC GTCCTCCTCG AAGAGCTCTC CAAAAACACG 
GACTACTCCA CGGACCTACG GATATCACTG GGATATTATA TTGAAGAGCT ACTGCGCCAC 
TGCTTAGGAC GAAATGATGT CTCTCGAAGA TCGGCCATCG TGGCCGCAGG AAGTGCCCTC 
CAGTACTGCG GCAAGATCTA TGGAGACCGA GTGGAGTACC TGTGCCAGGT GGTGGAGCAC 
CAGATCGAAG CCCTGCTCAC ATCGGAGTTG CAGAAGGAGA CCCCTAGCGG AAGTGCTGCA 
CCGGAGAAAA ACGAGCGCAG GCCTGAGGAA ACGCGCAAAC GTCACCCTAA AAAGCTAACT 
AACAAGGAAG TTGATCCCTA TTTGCTCACG TTGGAACCGA AAAGGTTTAA AACCATGTCG 
GACGACAAGC GCTTCAACGC GGCGGGCTTT GTAAAGTGCA CGAGAAATCG GACGATAGAA 
TACCTTTATC AGGATCACAC GCCGCCCAAC CTGTGGAAAC ACGCGCCAAT TGTGGATCCT 
CACAATCCCT ACGACCAGGA TGAGAAAAAG CAATACAAAA TGTTTACGTA TCACGTAGAG 
CATAGATACA ATACGCTTCT GCCAGATATT CCCTTCGAAA GGCTGAATCT CATTAAAGAA 
TATGTTCACA CAAATCAGGT GAACACCACT GAGATTCTTA ATGAGCACAT GACCACAAAG 
GAATATCTGG ACGAGTATAT TGCGCTGGAG AATCAAATGC TAGCAGCCCG CTATGGAGCC 
ATTGTCACAA GGAGAAGAAG ATTGGTTGAT TCCGCGAGAT TTATGGAGGA CAATTTGGAT 
GAGGGACTGG CGAAAAAAAT GTGCATGGAC AAGAATCTGC CAATGGACAC GAACGAAACT 
GTTCTTATTG ACCAATCTTT GGTGGATGAA AACAGCAGGT CGTTAACCAC AGCCGAGTCT 
ACGATGGGCA TCAGTCAGGC GGAGAACTCT ACTTTAAAGA GTAGCGAAGT CGAGGCGACT 
TTAAGCGTCA GCCATATAGA AAACTCCTTA AGCGATAGCC AAGAAGAGAA TCCTCCGCTG 
AGCAGCACAT TAGCCATTAA CGAGTCTAGT GTTCTCGACA GCACGAGAGT GGATCCCATA 
AAAGATCTCA CTCTAGATGA ACTGTTGCTA ATCGATTCAG GGATCAGTAT GGAAGAGCTT 
TCCGATATTC AAATGCATAC AGCAGGCCAG TCTTTTGATG ATGAGGGTGT TGTTCTTTCA 
GATTTAGAAG ATCAACGCCA GTTGTCGCCC ATGTTGCAGA TGGTTAGCCC ATCGATGGAG 
GCAAAAACGC TCAATATCAT TGAGATGGAT GCTGATCTTA TCATGAATGT TCCAAGGGAG 
GTGAGCTATC CTATTCTGCT GAATGTTATG GGTCTCCCCA TAAAACGCCT GCGTCGGAAA 
TGCATCTTTA AACTTCCACC TGAGTTCGAC TTATTCAGGC AAGCACGCCT TCCTATTAAG 
CGAGAGGGAC AACAAAAATC CCCAACCACT CCCAGAACTG TACTGCAAAT TGGAAGAGAG 
GCTCCCCAAA ATGAAAGAGA ACCCGGCTCC CCCTGTAGTT TAGAATTTGA TGAGGACTTG 
AATTTTCTGG GTTTCCGCCA ACGTCGACCC ACATTTGACT CGGGCTTCGA TATCGAAGAG 
CCGCGTGTAT CAAGTTGTGT CTCCACTATC GGAGAAGTTA AAACTGAGCT TGAAGATGAG 
ATTGAAGCCA ACACCAATAA TTTAACTGAC GCGAGCGCCA GCGACGCCTT GAATGAGAAT 
GTGAACGATA CAAAGGAAAG TGGGCTGGGA GATTCACTAG CGCAGGAATT AAACGCTTCC 
ACCGAACCCA ATGCGGAAAA CAATACCGCA AATTCATCGC AACTTGAAGT GACTGCTACA 
GAATCCTCCG GAACGCTAAG CGGACTGGAA ACCACAGTGG ATTTAGGAAT GGAGAGCGCG 
TTAGTTAGTT CTGTATTGGA TTTAAGTACC GAACCGAGTG CAATGGATAG TAACATTATA 
AATGTCACCC AACCTGACAA TTCAGACCTA GAGATTTCAA CCGTGCAGGA CCAAGATACC 
TTACATGACG AAGCAGATGC AAATGATTTG TCTGCTGAAA CACCCAATCC AATTGCCGAC 
TCAGCAGTCG ACGACTGCTC ATTGATCCGG GACTGGCATA GACGATTGGC TCCTGCTTTG 
GAAGCAGCCC ATGAACGGCA GAATTTCAAT ATTAAGGACT TGGGCACCGA GATCCTGGAC 
ATTTGCAAGG CGGGCAACAG AACAGCCACG TTAGCCGACG TCATGGCTGA CAAGGATCCT 
AGTATCATGT GTCGCTACAT GCTGGCTTCC CTGGTACTGA CGAACCACGG AAACGTGTCT 
CTGGATTTTG AAAATCGCGA TAAAAGTAAG CCCATCGACA TGTCTCAGTT TCGAATGCAT 
TTAAAGAGTA TGAAGCGAAT GGAAATTAAT CCCGAGGACG ACGTGGGCAA CATAAATGCT 
GCCCAGAGCA AATCGACGCC GAGAAGTAAA CAGCTAAATG ATTCAGCTCA CAAGCCACGA 
ACAAGCACGA CGGCATCTGC ACCAACAAAA CGAAAGTCTG CGGAAAATTC TTTATCCGAG 
GTGTTTGCTA AAACTGTGCG ATTGATACAG CCCATCCCAA AGATGTGGCC TACTCCTTCT 
GATGCCGACT CGGGGATATC TTCGATGGGC TCATCATTGG CATCGACAGC CCGCCTGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cap-H2 ( NM_169359.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731503.2 Drosophila melanogaster chromosome associated protein H2, transcript variant G (Cap-H2), mRNA.

Price & Availability↑

CloneID	OFa08282
Clone ID Related Accession (Same CDS sequence)	NM_169359.2
Accession Version	NM_169359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chromosome associated protein H2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169359.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGGA TTTTGCCTGA AGAAGCGGAA GCGGCCTACT TGAGCGAAGC GCGGGAGCAG 
ATATTGGAAA TAGCCAAGAA TCGGCCGGGA ACCCAAGTGG CCAAGTGCAT CCGCGCCTAC 
GATGAGCAAC AGGACCTGGC CTCGCTAGTC GTCCTCCTCG AAGAGCTCTC CAAAAACACG 
GACTACTCCA CGGACCTACG GATATCACTG GGATATTATA TTGAAGAGCT ACTGCGCCAC 
TGCTTAGGAC GAAATGATGT CTCTCGAAGA TCGGCCATCG TGGCCGCAGG AAGTGCCCTC 
CAGTACTGCG GCAAGATCTA TGGAGACCGA GTGGAGTACC TGTGCCAGGT GGTGGAGCAC 
CAGATCGAAG CCCTGCTCAC ATCGGAGTTG CAGAAGGAGA CCCCTAGCGG AAGTGCTGCA 
CCGGAGAAAA ACGAGCGCAG GCCTGAGGAA ACGCGCAAAC GTCACCCTAA AAAGCTAACT 
AACAAGGAAG TTGATCCCTA TTTGCTCACG TTGGAACCGA AAAGGTTTAA AACCATGTCG 
GACGACAAGC GCTTCAACGC GGCGGGCTTT GTAAAGTGCA CGAGAAATCG GACGATAGAA 
TACCTTTATC AGGATCACAC GCCGCCCAAC CTGTGGAAAC ACGCGCCAAT TGTGGATCCT 
CACAATCCCT ACGACCAGGA TGAGAAAAAG CAATACAAAA TGTTTACGTA TCACGTAGAG 
CATAGATACA ATACGCTTCT GCCAGATATT CCCTTCGAAA GGCTGAATCT CATTAAAGAA 
TATGTTCACA CAAATCAGGT GAACACCACT GAGATTCTTA ATGAGCACAT GACCACAAAG 
GAATATCTGG ACGAGTATAT TGCGCTGGAG AATCAAATGC TAGCAGCCCG CTATGGAGCC 
ATTGTCACAA GGAGAAGAAG ATTGGTTGAT TCCGCGAGAT TTATGGAGGA CAATTTGGAT 
GAGGGACTGG CGAAAAAAAT GTGCATGGAC AAGAATCTGC CAATGGACAC GAACGAAACT 
GTTCTTATTG ACCAATCTTT GGTGGATGAA AACAGCAGGT CGTTAACCAC AGCCGAGTCT 
ACGATGGGCA TCAGTCAGGC GGAGAACTCT ACTTTAAAGA GTAGCGAAGT CGAGGCGACT 
TTAAGCGTCA GCCATATAGA AAACTCCTTA AGCGATAGCC AAGAAGAGAA TCCTCCGCTG 
AGCAGCACAT TAGCCATTAA CGAGTCTAGT GTTCTCGACA GCACGAGAGT GGATCCCATA 
AAAGATCTCA CTCTAGATGA ACTGTTGCTA ATCGATTCAG GGATCAGTAT GGAAGAGCTT 
TCCGATATTC AAATGCATAC AGGCCAGTCT TTTGATGATG AGGGTGTTGT TCTTTCAGAT 
TTAGAAGATC AACGCCAGTT GTCGCCCATG TTGCAGATGG TTAGCCCATC GATGGAGGCA 
AAAACGCTCA ATATCATTGA GATGGATGCT GATCTTATCA TGAATGTTCC AAGGGAGGTG 
AGCTATCCTA TTCTGCTGAA TGTTATGGGT CTCCCCATAA AACGCCTGCG TCGGAAATGC 
ATCTTTAAAC TTCCACCTGA GTTCGACTTA TTCAGGCAAG CACGCCTTCC TATTAAGCGA 
GAGGGACAAC AAAAATCCCC AACCACTCCC AGAACTGTAC TGCAAATTGG AAGAGAGGCT 
CCCCAAAATG AAAGAGAACC CGGCTCCCCC TGTAGTTTAG AATTTGATGA GGACTTGAAT 
TTTCTGGGTT TCCGCCAACG TCGACCCACA TTTGACTCGG GCTTCGATAT CGAAGAGCCG 
CGTGTATCAA GTTGTGTCTC CACTATCGGA GAAGTTAAAA CTGAGCTTGA AGATGAGATT 
GAAGCCAACA CCAATAATTT AACTGACGCG AGCGCCAGCG ACGCCTTGAA TGAGAATGTG 
AACGATACAA AGGAAAGTGG GCTGGGAGAT TCACTAGCGC AGGAATTAAA CGCTTCCACC 
GAACCCAATG CGGAAAACAA TACCGCAAAT TCATCGCAAC TTGAAGTGAC TGCTACAGAA 
TCCTCCGGAA CGCTAAGCGG ACTGGAAACC ACAGTGGATT TAGGAATGGA GAGCGCGTTA 
GTTAGTTCTG TATTGGATTT AAGTACCGAA CCGAGTGCAA TGGATAGTAA CATTATAAAT 
GTCACCCAAC CTGACAATTC AGACCTAGAG ATTTCAACCG TGCAGGACCA AGATACCTTA 
CATGACGAAG CAGATGCAAA TGATTTGTCT GCTGAAACAC CCAATCCAAT TGCCGACTCA 
GCAGTCGACG ACTGCTCATT GATCCGGGAC TGGCATAGAC GATTGGCTCC TGCTTTGGAA 
GCAGCCCATG AACGGCAGAA TTTCAATATT AAGGACTTGG GCACCGAGAT CCTGGACATT 
TGCAAGGCGG GCAACAGAAC AGCCACGTTA GCCGACGTCA TGGCTGACAA GGATCCTAGT 
ATCATGTGTC GCTACATGCT GGCTTCCCTG GTACTGACGA ACCACGGAAA CGTGTCTCTG 
GATTTTGAAA ATCGCGATAA AAGTAAGCCC ATCGACATGT CTCAGTTTCG AATGCATTTA 
AAGAGTATGA AGCGAATGGA AATTAATCCC GAGGACGACG TGGGCAACAT AAATGCTGCC 
CAGAGCAAAT CGACGCCGAG AAGTAAACAG CTAAATGATT CAGCTCACAA GCCACGAACA 
AGCACGACGG CATCTGCACC AACAAAACGA AAGTCTGCGG AAAATTCTTT ATCCGAGGTG 
TTTGCTAAAA CTGTGCGATT GATACAGCCC ATCCCAAAGA TGTGGCCTAC TCCTTCTGAT 
GCCGACTCGG GGATATCTTC GATGGGCTCA TCATTGGCAT CGACAGCCCG CCTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731503.2
CDS	274..3153
Translation	MERILPEEAEAAYLSEAREQILEIAKNRPGTQVAKCIRAYDEQQDLASLVVLLEELSKNTDYSTDLRISLGYYIEELLRHCLGRNDVSRRSAIVAAGSALQYCGKIYGDRVEYLCQVVEHQIEALLTSELQKETPSGSAAPEKNERRPEETRKRHPKKLTNKEVDPYLLTLEPKRFKTMSDDKRFNAAGFVKCTRNRTIEYLYQDHTPPNLWKHAPIVDPHNPYDQDEKKQYKMFTYHVEHRYNTLLPDIPFERLNLIKEYVHTNQVNTTEILNEHMTTKEYLDEYIALENQMLAARYGAIVTRRRRLVDSARFMEDNLDEGLAKKMCMDKNLPMDTNETVLIDQSLVDENSRSLTTAESTMGISQAENSTLKSSEVEATLSVSHIENSLSDSQEENPPLSSTLAINESSVLDSTRVDPIKDLTLDELLLIDSGISMEELSDIQMHTGQSFDDEGVVLSDLEDQRQLSPMLQMVSPSMEAKTLNIIEMDADLIMNVPREVSYPILLNVMGLPIKRLRRKCIFKLPPEFDLFRQARLPIKREGQQKSPTTPRTVLQIGREAPQNEREPGSPCSLEFDEDLNFLGFRQRRPTFDSGFDIEEPRVSSCVSTIGEVKTELEDEIEANTNNLTDASASDALNENVNDTKESGLGDSLAQELNASTEPNAENNTANSSQLEVTATESSGTLSGLETTVDLGMESALVSSVLDLSTEPSAMDSNIINVTQPDNSDLEISTVQDQDTLHDEADANDLSAETPNPIADSAVDDCSLIRDWHRRLAPALEAAHERQNFNIKDLGTEILDICKAGNRTATLADVMADKDPSIMCRYMLASLVLTNHGNVSLDFENRDKSKPIDMSQFRMHLKSMKRMEINPEDDVGNINAAQSKSTPRSKQLNDSAHKPRTSTTASAPTKRKSAENSLSEVFAKTVRLIQPIPKMWPTPSDADSGISSMGSSLASTARLK

Target ORF information:

RefSeq Version	NM_169359.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chromosome associated protein H2, transcript variant G (Cap-H2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169359.2

ORF Insert Sequence:

ATGGAGCGGA TTTTGCCTGA AGAAGCGGAA GCGGCCTACT TGAGCGAAGC GCGGGAGCAG 
ATATTGGAAA TAGCCAAGAA TCGGCCGGGA ACCCAAGTGG CCAAGTGCAT CCGCGCCTAC 
GATGAGCAAC AGGACCTGGC CTCGCTAGTC GTCCTCCTCG AAGAGCTCTC CAAAAACACG 
GACTACTCCA CGGACCTACG GATATCACTG GGATATTATA TTGAAGAGCT ACTGCGCCAC 
TGCTTAGGAC GAAATGATGT CTCTCGAAGA TCGGCCATCG TGGCCGCAGG AAGTGCCCTC 
CAGTACTGCG GCAAGATCTA TGGAGACCGA GTGGAGTACC TGTGCCAGGT GGTGGAGCAC 
CAGATCGAAG CCCTGCTCAC ATCGGAGTTG CAGAAGGAGA CCCCTAGCGG AAGTGCTGCA 
CCGGAGAAAA ACGAGCGCAG GCCTGAGGAA ACGCGCAAAC GTCACCCTAA AAAGCTAACT 
AACAAGGAAG TTGATCCCTA TTTGCTCACG TTGGAACCGA AAAGGTTTAA AACCATGTCG 
GACGACAAGC GCTTCAACGC GGCGGGCTTT GTAAAGTGCA CGAGAAATCG GACGATAGAA 
TACCTTTATC AGGATCACAC GCCGCCCAAC CTGTGGAAAC ACGCGCCAAT TGTGGATCCT 
CACAATCCCT ACGACCAGGA TGAGAAAAAG CAATACAAAA TGTTTACGTA TCACGTAGAG 
CATAGATACA ATACGCTTCT GCCAGATATT CCCTTCGAAA GGCTGAATCT CATTAAAGAA 
TATGTTCACA CAAATCAGGT GAACACCACT GAGATTCTTA ATGAGCACAT GACCACAAAG 
GAATATCTGG ACGAGTATAT TGCGCTGGAG AATCAAATGC TAGCAGCCCG CTATGGAGCC 
ATTGTCACAA GGAGAAGAAG ATTGGTTGAT TCCGCGAGAT TTATGGAGGA CAATTTGGAT 
GAGGGACTGG CGAAAAAAAT GTGCATGGAC AAGAATCTGC CAATGGACAC GAACGAAACT 
GTTCTTATTG ACCAATCTTT GGTGGATGAA AACAGCAGGT CGTTAACCAC AGCCGAGTCT 
ACGATGGGCA TCAGTCAGGC GGAGAACTCT ACTTTAAAGA GTAGCGAAGT CGAGGCGACT 
TTAAGCGTCA GCCATATAGA AAACTCCTTA AGCGATAGCC AAGAAGAGAA TCCTCCGCTG 
AGCAGCACAT TAGCCATTAA CGAGTCTAGT GTTCTCGACA GCACGAGAGT GGATCCCATA 
AAAGATCTCA CTCTAGATGA ACTGTTGCTA ATCGATTCAG GGATCAGTAT GGAAGAGCTT 
TCCGATATTC AAATGCATAC AGGCCAGTCT TTTGATGATG AGGGTGTTGT TCTTTCAGAT 
TTAGAAGATC AACGCCAGTT GTCGCCCATG TTGCAGATGG TTAGCCCATC GATGGAGGCA 
AAAACGCTCA ATATCATTGA GATGGATGCT GATCTTATCA TGAATGTTCC AAGGGAGGTG 
AGCTATCCTA TTCTGCTGAA TGTTATGGGT CTCCCCATAA AACGCCTGCG TCGGAAATGC 
ATCTTTAAAC TTCCACCTGA GTTCGACTTA TTCAGGCAAG CACGCCTTCC TATTAAGCGA 
GAGGGACAAC AAAAATCCCC AACCACTCCC AGAACTGTAC TGCAAATTGG AAGAGAGGCT 
CCCCAAAATG AAAGAGAACC CGGCTCCCCC TGTAGTTTAG AATTTGATGA GGACTTGAAT 
TTTCTGGGTT TCCGCCAACG TCGACCCACA TTTGACTCGG GCTTCGATAT CGAAGAGCCG 
CGTGTATCAA GTTGTGTCTC CACTATCGGA GAAGTTAAAA CTGAGCTTGA AGATGAGATT 
GAAGCCAACA CCAATAATTT AACTGACGCG AGCGCCAGCG ACGCCTTGAA TGAGAATGTG 
AACGATACAA AGGAAAGTGG GCTGGGAGAT TCACTAGCGC AGGAATTAAA CGCTTCCACC 
GAACCCAATG CGGAAAACAA TACCGCAAAT TCATCGCAAC TTGAAGTGAC TGCTACAGAA 
TCCTCCGGAA CGCTAAGCGG ACTGGAAACC ACAGTGGATT TAGGAATGGA GAGCGCGTTA 
GTTAGTTCTG TATTGGATTT AAGTACCGAA CCGAGTGCAA TGGATAGTAA CATTATAAAT 
GTCACCCAAC CTGACAATTC AGACCTAGAG ATTTCAACCG TGCAGGACCA AGATACCTTA 
CATGACGAAG CAGATGCAAA TGATTTGTCT GCTGAAACAC CCAATCCAAT TGCCGACTCA 
GCAGTCGACG ACTGCTCATT GATCCGGGAC TGGCATAGAC GATTGGCTCC TGCTTTGGAA 
GCAGCCCATG AACGGCAGAA TTTCAATATT AAGGACTTGG GCACCGAGAT CCTGGACATT 
TGCAAGGCGG GCAACAGAAC AGCCACGTTA GCCGACGTCA TGGCTGACAA GGATCCTAGT 
ATCATGTGTC GCTACATGCT GGCTTCCCTG GTACTGACGA ACCACGGAAA CGTGTCTCTG 
GATTTTGAAA ATCGCGATAA AAGTAAGCCC ATCGACATGT CTCAGTTTCG AATGCATTTA 
AAGAGTATGA AGCGAATGGA AATTAATCCC GAGGACGACG TGGGCAACAT AAATGCTGCC 
CAGAGCAAAT CGACGCCGAG AAGTAAACAG CTAAATGATT CAGCTCACAA GCCACGAACA 
AGCACGACGG CATCTGCACC AACAAAACGA AAGTCTGCGG AAAATTCTTT ATCCGAGGTG 
TTTGCTAAAA CTGTGCGATT GATACAGCCC ATCCCAAAGA TGTGGCCTAC TCCTTCTGAT 
GCCGACTCGG GGATATCTTC GATGGGCTCA TCATTGGCAT CGACAGCCCG CCTGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cap-H2 ( NM_169358.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731502.2 Drosophila melanogaster chromosome associated protein H2, transcript variant F (Cap-H2), mRNA.

Price & Availability↑

CloneID	OFa08283
Clone ID Related Accession (Same CDS sequence)	NM_169358.2
Accession Version	NM_169358.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chromosome associated protein H2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_169358.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGGA TTTTGCCTGA AGAAGCGGAA GCGGCCTACT TGAGCGAAGC GCGGGAGCAG 
ATATTGGAAA TAGCCAAGAA TCGGCCGGGA ACCCAAGTGG CCAAGTGCAT CCGCGCCTAC 
GATGAGCAAC AGGACCTGGC CTCGCTAGTC GTCCTCCTCG AAGAGCTCTC CAAAAACACG 
GACTACTCCA CGGACCTACG GATATCACTG GGATATTATA TTGAAGAGCT ACTGCGCCAC 
TGCTTAGGAC GAAATGATGT CTCTCGAAGA TCGGCCATCG TGGCCGCAGG AAGTGCCCTC 
CAGTACTGCG GCAAGATCTA TGGAGACCGA GTGGAGTACC TGTGCCAGGT GGTGGAGCAC 
CAGATCGAAG CCCTGCTCAC ATCGGAGTTG CAGAAGGAGA CCCCTAGCGG AAGTGCTGCA 
CCGGAGAAAA ACGAGCGCAG GCCTGAGGAA ACGCGCAAAC GTCACCCTAA AAAGCTAACT 
AACAAGGAAG TTGATCCCTA TTTGCTCACG TTGGAACCGA AAAGGTTTAA AACCATGTCG 
GACGACAAGC GCTTCAACGC GGCGGGCTTT GTAAAGTGCA CGAGAAATCG GACGATAGAA 
TACCTTTATC AGGATCACAC GCCGCCCAAC CTGTGGAAAC ACGCGCCAAT TGTGGATCCT 
CACAATCCCT ACGACCAGGA TGAGAAAAAG CAATACAAAA TGTTTACGTA TCACGTAGAG 
CATAGATACA ATACGCTTCT GCCAGATATT CCCTTCGAAA GGCTGAATCT CATTAAAGAA 
TATGTTCACA CAAATCAGGT GAACACCACT GAGATTCTTA ATGAGCACAT GACCACAAAG 
GAATATCTGG ACGAGTATAT TGCGCTGGAG AATCAAATGC TAGCAGCCCG CTATGGAGCC 
ATTGTCACAA GGAGAAGAAG ATTGGTTGAT TCCGCGAGAT TTATGGAGGA CAATTTGGAT 
GAGGGACTGG CGAAAAAAAT GTGCATGGAC AAGAATCTGC CAATGGACAC GAACGAAACT 
GTTCTTATTG ACCAATCTTT GGTGGATGAA AACAGCAGGT CGTTAACCAC AGCCGAGTCT 
ACGATGGGCA TCAGTCAGGC GGAGAACTCT ACTTTAAAGA GTAGCGAAGT CGAGGCGACT 
TTAAGCGTCA GCCATATAGA AAACTCCTTA AGCGATAGCC AAGAAGAGAA TCCTCCGCTG 
AGCAGCACAT TAGCCATTAA CGAGTCTAGT GTTCTCGACA GCACGAGAGT GGATCCCATA 
AAAGATCTCA CTCTAGATGA ACTGTTGCTA ATCGATTCAG GGATCAGTAT GGAAGAGCTT 
TCCGATATTC AAATGCATAC AGATTTAGAA GATCAACGCC AGTTGTCGCC CATGTTGCAG 
ATGGTTAGCC CATCGATGGA GGCAAAAACG CTCAATATCA TTGAGATGGA TGCTGATCTT 
ATCATGAATG TTCCAAGGGA GGTGAGCTAT CCTATTCTGC TGAATGTTAT GGGTCTCCCC 
ATAAAACGCC TGCGTCGGAA ATGCATCTTT AAACTTCCAC CTGAGTTCGA CTTATTCAGG 
CAAGCACGCC TTCCTATTAA GCGAGAGGGA CAACAAAAAT CCCCAACCAC TCCCAGAACT 
GTACTGCAAA TTGGAAGAGA GGCTCCCCAA AATGAAAGAG AACCCGGCTC CCCCTGTAGT 
TTAGAATTTG ATGAGGACTT GAATTTTCTG GGTTTCCGCC AACGTCGACC CACATTTGAC 
TCGGGCTTCG ATATCGAAGA GCCGCGTGTA TCAAGTTGTG TCTCCACTAT CGGAGAAGTT 
AAAACTGAGC TTGAAGATGA GATTGAAGCC AACACCAATA ATTTAACTGA CGCGAGCGCC 
AGCGACGCCT TGAATGAGAA TGTGAACGAT ACAAAGGAAA GTGGGCTGGG AGATTCACTA 
GCGCAGGAAT TAAACGCTTC CACCGAACCC AATGCGGAAA ACAATACCGC AAATTCATCG 
CAACTTGAAG TGACTGCTAC AGAATCCTCC GGAACGCTAA GCGGACTGGA AACCACAGTG 
GATTTAGGAA TGGAGAGCGC GTTAGTTAGT TCTGTATTGG ATTTAAGTAC CGAACCGAGT 
GCAATGGATA GTAACATTAT AAATGTCACC CAACCTGACA ATTCAGACCT AGAGATTTCA 
ACCGTGCAGG ACCAAGATAC CTTACATGAC GAAGCAGATG CAAATGATTT GTCTGCTGAA 
ACACCCAATC CAATTGCCGA CTCAGCAGTC GACGACTGCT CATTGATCCG GGACTGGCAT 
AGACGATTGG CTCCTGCTTT GGAAGCAGCC CATGAACGGC AGAATTTCAA TATTAAGGAC 
TTGGGCACCG AGATCCTGGA CATTTGCAAG GCGGGCAACA GAACAGCCAC GTTAGCCGAC 
GTCATGGCTG ACAAGGATCC TAGTATCATG TGTCGCTACA TGCTGGCTTC CCTGGTACTG 
ACGAACCACG GAAACGTGTC TCTGGATTTT GAAAATCGCG ATAAAAGTAA GCCCATCGAC 
ATGTCTCAGT TTCGAATGCA TTTAAAGAGT ATGAAGCGAA TGGAAATTAA TCCCGAGGAC 
GACGTGGGCA ACATAAATGC TGCCCAGAGC AAATCGACGC CGAGAAGTAA ACAGCTAAAT 
GATTCAGCTC ACAAGCCACG AACAAGCACG ACGGCATCTG CACCAACAAA ACGAAAGTCT 
GCGGAAAATT CTTTATCCGA GGTGTTTGCT AAAACTGTGC GATTGATACA GCCCATCCCA 
AAGATGTGGC CTACTCCTTC TGATGCCGAC TCGGGGATAT CTTCGATGGG CTCATCATTG 
GCATCGACAG CCCGCCTGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731502.2
CDS	274..3117
Translation	MERILPEEAEAAYLSEAREQILEIAKNRPGTQVAKCIRAYDEQQDLASLVVLLEELSKNTDYSTDLRISLGYYIEELLRHCLGRNDVSRRSAIVAAGSALQYCGKIYGDRVEYLCQVVEHQIEALLTSELQKETPSGSAAPEKNERRPEETRKRHPKKLTNKEVDPYLLTLEPKRFKTMSDDKRFNAAGFVKCTRNRTIEYLYQDHTPPNLWKHAPIVDPHNPYDQDEKKQYKMFTYHVEHRYNTLLPDIPFERLNLIKEYVHTNQVNTTEILNEHMTTKEYLDEYIALENQMLAARYGAIVTRRRRLVDSARFMEDNLDEGLAKKMCMDKNLPMDTNETVLIDQSLVDENSRSLTTAESTMGISQAENSTLKSSEVEATLSVSHIENSLSDSQEENPPLSSTLAINESSVLDSTRVDPIKDLTLDELLLIDSGISMEELSDIQMHTDLEDQRQLSPMLQMVSPSMEAKTLNIIEMDADLIMNVPREVSYPILLNVMGLPIKRLRRKCIFKLPPEFDLFRQARLPIKREGQQKSPTTPRTVLQIGREAPQNEREPGSPCSLEFDEDLNFLGFRQRRPTFDSGFDIEEPRVSSCVSTIGEVKTELEDEIEANTNNLTDASASDALNENVNDTKESGLGDSLAQELNASTEPNAENNTANSSQLEVTATESSGTLSGLETTVDLGMESALVSSVLDLSTEPSAMDSNIINVTQPDNSDLEISTVQDQDTLHDEADANDLSAETPNPIADSAVDDCSLIRDWHRRLAPALEAAHERQNFNIKDLGTEILDICKAGNRTATLADVMADKDPSIMCRYMLASLVLTNHGNVSLDFENRDKSKPIDMSQFRMHLKSMKRMEINPEDDVGNINAAQSKSTPRSKQLNDSAHKPRTSTTASAPTKRKSAENSLSEVFAKTVRLIQPIPKMWPTPSDADSGISSMGSSLASTARLK

Target ORF information:

RefSeq Version	NM_169358.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chromosome associated protein H2, transcript variant F (Cap-H2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169358.2

ORF Insert Sequence:

ATGGAGCGGA TTTTGCCTGA AGAAGCGGAA GCGGCCTACT TGAGCGAAGC GCGGGAGCAG 
ATATTGGAAA TAGCCAAGAA TCGGCCGGGA ACCCAAGTGG CCAAGTGCAT CCGCGCCTAC 
GATGAGCAAC AGGACCTGGC CTCGCTAGTC GTCCTCCTCG AAGAGCTCTC CAAAAACACG 
GACTACTCCA CGGACCTACG GATATCACTG GGATATTATA TTGAAGAGCT ACTGCGCCAC 
TGCTTAGGAC GAAATGATGT CTCTCGAAGA TCGGCCATCG TGGCCGCAGG AAGTGCCCTC 
CAGTACTGCG GCAAGATCTA TGGAGACCGA GTGGAGTACC TGTGCCAGGT GGTGGAGCAC 
CAGATCGAAG CCCTGCTCAC ATCGGAGTTG CAGAAGGAGA CCCCTAGCGG AAGTGCTGCA 
CCGGAGAAAA ACGAGCGCAG GCCTGAGGAA ACGCGCAAAC GTCACCCTAA AAAGCTAACT 
AACAAGGAAG TTGATCCCTA TTTGCTCACG TTGGAACCGA AAAGGTTTAA AACCATGTCG 
GACGACAAGC GCTTCAACGC GGCGGGCTTT GTAAAGTGCA CGAGAAATCG GACGATAGAA 
TACCTTTATC AGGATCACAC GCCGCCCAAC CTGTGGAAAC ACGCGCCAAT TGTGGATCCT 
CACAATCCCT ACGACCAGGA TGAGAAAAAG CAATACAAAA TGTTTACGTA TCACGTAGAG 
CATAGATACA ATACGCTTCT GCCAGATATT CCCTTCGAAA GGCTGAATCT CATTAAAGAA 
TATGTTCACA CAAATCAGGT GAACACCACT GAGATTCTTA ATGAGCACAT GACCACAAAG 
GAATATCTGG ACGAGTATAT TGCGCTGGAG AATCAAATGC TAGCAGCCCG CTATGGAGCC 
ATTGTCACAA GGAGAAGAAG ATTGGTTGAT TCCGCGAGAT TTATGGAGGA CAATTTGGAT 
GAGGGACTGG CGAAAAAAAT GTGCATGGAC AAGAATCTGC CAATGGACAC GAACGAAACT 
GTTCTTATTG ACCAATCTTT GGTGGATGAA AACAGCAGGT CGTTAACCAC AGCCGAGTCT 
ACGATGGGCA TCAGTCAGGC GGAGAACTCT ACTTTAAAGA GTAGCGAAGT CGAGGCGACT 
TTAAGCGTCA GCCATATAGA AAACTCCTTA AGCGATAGCC AAGAAGAGAA TCCTCCGCTG 
AGCAGCACAT TAGCCATTAA CGAGTCTAGT GTTCTCGACA GCACGAGAGT GGATCCCATA 
AAAGATCTCA CTCTAGATGA ACTGTTGCTA ATCGATTCAG GGATCAGTAT GGAAGAGCTT 
TCCGATATTC AAATGCATAC AGATTTAGAA GATCAACGCC AGTTGTCGCC CATGTTGCAG 
ATGGTTAGCC CATCGATGGA GGCAAAAACG CTCAATATCA TTGAGATGGA TGCTGATCTT 
ATCATGAATG TTCCAAGGGA GGTGAGCTAT CCTATTCTGC TGAATGTTAT GGGTCTCCCC 
ATAAAACGCC TGCGTCGGAA ATGCATCTTT AAACTTCCAC CTGAGTTCGA CTTATTCAGG 
CAAGCACGCC TTCCTATTAA GCGAGAGGGA CAACAAAAAT CCCCAACCAC TCCCAGAACT 
GTACTGCAAA TTGGAAGAGA GGCTCCCCAA AATGAAAGAG AACCCGGCTC CCCCTGTAGT 
TTAGAATTTG ATGAGGACTT GAATTTTCTG GGTTTCCGCC AACGTCGACC CACATTTGAC 
TCGGGCTTCG ATATCGAAGA GCCGCGTGTA TCAAGTTGTG TCTCCACTAT CGGAGAAGTT 
AAAACTGAGC TTGAAGATGA GATTGAAGCC AACACCAATA ATTTAACTGA CGCGAGCGCC 
AGCGACGCCT TGAATGAGAA TGTGAACGAT ACAAAGGAAA GTGGGCTGGG AGATTCACTA 
GCGCAGGAAT TAAACGCTTC CACCGAACCC AATGCGGAAA ACAATACCGC AAATTCATCG 
CAACTTGAAG TGACTGCTAC AGAATCCTCC GGAACGCTAA GCGGACTGGA AACCACAGTG 
GATTTAGGAA TGGAGAGCGC GTTAGTTAGT TCTGTATTGG ATTTAAGTAC CGAACCGAGT 
GCAATGGATA GTAACATTAT AAATGTCACC CAACCTGACA ATTCAGACCT AGAGATTTCA 
ACCGTGCAGG ACCAAGATAC CTTACATGAC GAAGCAGATG CAAATGATTT GTCTGCTGAA 
ACACCCAATC CAATTGCCGA CTCAGCAGTC GACGACTGCT CATTGATCCG GGACTGGCAT 
AGACGATTGG CTCCTGCTTT GGAAGCAGCC CATGAACGGC AGAATTTCAA TATTAAGGAC 
TTGGGCACCG AGATCCTGGA CATTTGCAAG GCGGGCAACA GAACAGCCAC GTTAGCCGAC 
GTCATGGCTG ACAAGGATCC TAGTATCATG TGTCGCTACA TGCTGGCTTC CCTGGTACTG 
ACGAACCACG GAAACGTGTC TCTGGATTTT GAAAATCGCG ATAAAAGTAA GCCCATCGAC 
ATGTCTCAGT TTCGAATGCA TTTAAAGAGT ATGAAGCGAA TGGAAATTAA TCCCGAGGAC 
GACGTGGGCA ACATAAATGC TGCCCAGAGC AAATCGACGC CGAGAAGTAA ACAGCTAAAT 
GATTCAGCTC ACAAGCCACG AACAAGCACG ACGGCATCTG CACCAACAAA ACGAAAGTCT 
GCGGAAAATT CTTTATCCGA GGTGTTTGCT AAAACTGTGC GATTGATACA GCCCATCCCA 
AAGATGTGGC CTACTCCTTC TGATGCCGAC TCGGGGATAT CTTCGATGGG CTCATCATTG 
GCATCGACAG CCCGCCTGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cap-H2 ( NM_206471.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996193.1 Drosophila melanogaster chromosome associated protein H2, transcript variant D (Cap-H2), mRNA.

Price & Availability↑

CloneID	OFa08284
Clone ID Related Accession (Same CDS sequence)	NM_206471.2
Accession Version	NM_206471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chromosome associated protein H2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_206471.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACG ACAAGCGCTT CAACGCGGCG GGCTTTGTAA AGTGCACGAG AAATCGGACG 
ATAGAATACC TTTATCAGGA TCACACGCCG CCCAACCTGT GGAAACACGC GCCAATTGTG 
GATCCTCACA ATCCCTACGA CCAGGATGAG AAAAAGCAAT ACAAAATGTT TACGTATCAC 
GTAGAGCATA GATACAATAC GCTTCTGCCA GATATTCCCT TCGAAAGGCT GAATCTCATT 
AAAGAATATG TTCACACAAA TCAGGTGAAC ACCACTGAGA TTCTTAATGA GCACATGACC 
ACAAAGGAAT ATCTGGACGA GTATATTGCG CTGGAGAATC AAATGCTAGC AGCCCGCTAT 
GGAGCCATTG TCACAAGGAG AAGAAGATTG GTTGATTCCG CGAGATTTAT GGAGGACAAT 
TTGGATGAGG GACTGGCGAA AAAAATGTGC ATGGACAAGA ATCTGCCAAT GGACACGAAC 
GAAACTGTTC TTATTGACCA ATCTTTGGTG GATGAAAACA GCAGGTCGTT AACCACAGCC 
GAGTCTACGA TGGGCATCAG TCAGGCGGAG AACTCTACTT TAAAGAGTAG CGAAGTCGAG 
GCGACTTTAA GCGTCAGCCA TATAGAAAAC TCCTTAAGCG ATAGCCAAGA AGAGAATCCT 
CCGCTGAGCA GCACATTAGC CATTAACGAG TCTAGTGTTC TCGACAGCAC GAGAGTGGAT 
CCCATAAAAG ATCTCACTCT AGATGAACTG TTGCTAATCG ATTCAGGGAT CAGTATGGAA 
GAGCTTTCCG ATATTCAAAT GCATACAGAT TTAGAAGATC AACGCCAGTT GTCGCCCATG 
TTGCAGATGG TTAGCCCATC GATGGAGGCA AAAACGCTCA ATATCATTGA GATGGATGCT 
GATCTTATCA TGAATGTTCC AAGGGAGGTG AGCTATCCTA TTCTGCTGAA TGTTATGGGT 
CTCCCCATAA AACGCCTGCG TCGGAAATGC ATCTTTAAAC TTCCACCTGA GTTCGACTTA 
TTCAGGCAAG CACGCCTTCC TATTAAGCGA GAGGGACAAC AAAAATCCCC AACCACTCCC 
AGAACTGTAC TGCAAATTGG AAGAGAGGCT CCCCAAAATG AAAGAGAACC CGGCTCCCCC 
TGTAGTTTAG AATTTGATGA GGACTTGAAT TTTCTGGGTT TCCGCCAACG TCGACCCACA 
TTTGACTCGG GCTTCGATAT CGAAGAGCCG CGTGTATCAA GTTGTGTCTC CACTATCGGA 
GAAGTTAAAA CTGAGCTTGA AGATGAGATT GAAGCCAACA CCAATAATTT AACTGACGCG 
AGCGCCAGCG ACGCCTTGAA TGAGAATGTG AACGATACAA AGGAAAGTGG GCTGGGAGAT 
TCACTAGCGC AGGAATTAAA CGCTTCCACC GAACCCAATG CGGAAAACAA TACCGCAAAT 
TCATCGCAAC TTGAAGTGAC TGCTACAGAA TCCTCCGGAA CGCTAAGCGG ACTGGAAACC 
ACAGTGGATT TAGGAATGGA GAGCGCGTTA GTTAGTTCTG TATTGGATTT AAGTACCGAA 
CCGAGTGCAA TGGATAGTAA CATTATAAAT GTCACCCAAC CTGACAATTC AGACCTAGAG 
ATTTCAACCG TGCAGGACCA AGATACCTTA CATGACGAAG CAGATGCAAA TGATTTGTCT 
GCTGAAACAC CCAATCCAAT TGCCGACTCA GCAGTCGACG ACTGCTCATT GATCCGGGAC 
TGGCATAGAC GATTGGCTCC TGCTTTGGAA GCAGCCCATG AACGGCAGAA TTTCAATATT 
AAGGACTTGG GCACCGAGAT CCTGGACATT TGCAAGGCGG GCAACAGAAC AGCCACGTTA 
GCCGACGTCA TGGCTGACAA GGATCCTAGT ATCATGTGTC GCTACATGCT GGCTTCCCTG 
GTACTGACGA ACCACGGAAA CGTGTCTCTG GATTTTGAAA ATCGCGATAA AAGCCGAGAG 
ATCAATGCGC TTTCCCATGC GGTACAACAA GATCAAGAGG CTGCATTAGA TATATGTTCT 
TGCCAGATTA TTTACCCGCA ATCGTGTACA AATAACAAAA ATAGACAAAT GACTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996193.1
CDS	573..2672
Translation	MSDDKRFNAAGFVKCTRNRTIEYLYQDHTPPNLWKHAPIVDPHNPYDQDEKKQYKMFTYHVEHRYNTLLPDIPFERLNLIKEYVHTNQVNTTEILNEHMTTKEYLDEYIALENQMLAARYGAIVTRRRRLVDSARFMEDNLDEGLAKKMCMDKNLPMDTNETVLIDQSLVDENSRSLTTAESTMGISQAENSTLKSSEVEATLSVSHIENSLSDSQEENPPLSSTLAINESSVLDSTRVDPIKDLTLDELLLIDSGISMEELSDIQMHTDLEDQRQLSPMLQMVSPSMEAKTLNIIEMDADLIMNVPREVSYPILLNVMGLPIKRLRRKCIFKLPPEFDLFRQARLPIKREGQQKSPTTPRTVLQIGREAPQNEREPGSPCSLEFDEDLNFLGFRQRRPTFDSGFDIEEPRVSSCVSTIGEVKTELEDEIEANTNNLTDASASDALNENVNDTKESGLGDSLAQELNASTEPNAENNTANSSQLEVTATESSGTLSGLETTVDLGMESALVSSVLDLSTEPSAMDSNIINVTQPDNSDLEISTVQDQDTLHDEADANDLSAETPNPIADSAVDDCSLIRDWHRRLAPALEAAHERQNFNIKDLGTEILDICKAGNRTATLADVMADKDPSIMCRYMLASLVLTNHGNVSLDFENRDKSREINALSHAVQQDQEAALDICSCQIIYPQSCTNNKNRQMTF

Target ORF information:

RefSeq Version	NM_206471.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chromosome associated protein H2, transcript variant D (Cap-H2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206471.2

ORF Insert Sequence:

ATGTCGGACG ACAAGCGCTT CAACGCGGCG GGCTTTGTAA AGTGCACGAG AAATCGGACG 
ATAGAATACC TTTATCAGGA TCACACGCCG CCCAACCTGT GGAAACACGC GCCAATTGTG 
GATCCTCACA ATCCCTACGA CCAGGATGAG AAAAAGCAAT ACAAAATGTT TACGTATCAC 
GTAGAGCATA GATACAATAC GCTTCTGCCA GATATTCCCT TCGAAAGGCT GAATCTCATT 
AAAGAATATG TTCACACAAA TCAGGTGAAC ACCACTGAGA TTCTTAATGA GCACATGACC 
ACAAAGGAAT ATCTGGACGA GTATATTGCG CTGGAGAATC AAATGCTAGC AGCCCGCTAT 
GGAGCCATTG TCACAAGGAG AAGAAGATTG GTTGATTCCG CGAGATTTAT GGAGGACAAT 
TTGGATGAGG GACTGGCGAA AAAAATGTGC ATGGACAAGA ATCTGCCAAT GGACACGAAC 
GAAACTGTTC TTATTGACCA ATCTTTGGTG GATGAAAACA GCAGGTCGTT AACCACAGCC 
GAGTCTACGA TGGGCATCAG TCAGGCGGAG AACTCTACTT TAAAGAGTAG CGAAGTCGAG 
GCGACTTTAA GCGTCAGCCA TATAGAAAAC TCCTTAAGCG ATAGCCAAGA AGAGAATCCT 
CCGCTGAGCA GCACATTAGC CATTAACGAG TCTAGTGTTC TCGACAGCAC GAGAGTGGAT 
CCCATAAAAG ATCTCACTCT AGATGAACTG TTGCTAATCG ATTCAGGGAT CAGTATGGAA 
GAGCTTTCCG ATATTCAAAT GCATACAGAT TTAGAAGATC AACGCCAGTT GTCGCCCATG 
TTGCAGATGG TTAGCCCATC GATGGAGGCA AAAACGCTCA ATATCATTGA GATGGATGCT 
GATCTTATCA TGAATGTTCC AAGGGAGGTG AGCTATCCTA TTCTGCTGAA TGTTATGGGT 
CTCCCCATAA AACGCCTGCG TCGGAAATGC ATCTTTAAAC TTCCACCTGA GTTCGACTTA 
TTCAGGCAAG CACGCCTTCC TATTAAGCGA GAGGGACAAC AAAAATCCCC AACCACTCCC 
AGAACTGTAC TGCAAATTGG AAGAGAGGCT CCCCAAAATG AAAGAGAACC CGGCTCCCCC 
TGTAGTTTAG AATTTGATGA GGACTTGAAT TTTCTGGGTT TCCGCCAACG TCGACCCACA 
TTTGACTCGG GCTTCGATAT CGAAGAGCCG CGTGTATCAA GTTGTGTCTC CACTATCGGA 
GAAGTTAAAA CTGAGCTTGA AGATGAGATT GAAGCCAACA CCAATAATTT AACTGACGCG 
AGCGCCAGCG ACGCCTTGAA TGAGAATGTG AACGATACAA AGGAAAGTGG GCTGGGAGAT 
TCACTAGCGC AGGAATTAAA CGCTTCCACC GAACCCAATG CGGAAAACAA TACCGCAAAT 
TCATCGCAAC TTGAAGTGAC TGCTACAGAA TCCTCCGGAA CGCTAAGCGG ACTGGAAACC 
ACAGTGGATT TAGGAATGGA GAGCGCGTTA GTTAGTTCTG TATTGGATTT AAGTACCGAA 
CCGAGTGCAA TGGATAGTAA CATTATAAAT GTCACCCAAC CTGACAATTC AGACCTAGAG 
ATTTCAACCG TGCAGGACCA AGATACCTTA CATGACGAAG CAGATGCAAA TGATTTGTCT 
GCTGAAACAC CCAATCCAAT TGCCGACTCA GCAGTCGACG ACTGCTCATT GATCCGGGAC 
TGGCATAGAC GATTGGCTCC TGCTTTGGAA GCAGCCCATG AACGGCAGAA TTTCAATATT 
AAGGACTTGG GCACCGAGAT CCTGGACATT TGCAAGGCGG GCAACAGAAC AGCCACGTTA 
GCCGACGTCA TGGCTGACAA GGATCCTAGT ATCATGTGTC GCTACATGCT GGCTTCCCTG 
GTACTGACGA ACCACGGAAA CGTGTCTCTG GATTTTGAAA ATCGCGATAA AAGCCGAGAG 
ATCAATGCGC TTTCCCATGC GGTACAACAA GATCAAGAGG CTGCATTAGA TATATGTTCT 
TGCCAGATTA TTTACCCGCA ATCGTGTACA AATAACAAAA ATAGACAAAT GACTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cap-H2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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