ORF » Species Summary » Drosophila melanogaster » Nuak1 cDNA ORF clone

Nuak1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nuak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nuak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04447 NM_206469.3
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa08254 NM_001260091.1
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa04448 NM_206470.1
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa04448 NM_169337.2
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa04448 NM_141734.2
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa08252 NM_001260090.1
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08253 NM_001275528.1
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa04449 NM_141736.3
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OFa08251 NM_001275529.1
Latest version!		 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Nuak1 ( NM_206469.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996191.1 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant D, mRNA.
    Price & Availability↑
    CloneID OFa04447
    Clone ID Related Accession (Same CDS sequence) NM_206469.3
    Accession Version NM_206469.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_206469.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGCCGCCTG AACAGCAGCA ACAGCAGCGG ACTGGGAGCT 
    CCGTGGCGGC ACAGCATGTC CCGGGATCTG GGCAGCGACA TGGAATCGAT GTTCTCGCGC 
    ACGGGGGCCA CTCTGCCAAG AGGTGAATAT CACAGAATAA AGAGTAAATA CCTAGATCAG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996191.1
    CDS721..4263
    Translation

    Target ORF information:

    RefSeq Version NM_206469.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_206469.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGCCGCCTG AACAGCAGCA ACAGCAGCGG ACTGGGAGCT 
    CCGTGGCGGC ACAGCATGTC CCGGGATCTG GGCAGCGACA TGGAATCGAT GTTCTCGCGC 
    ACGGGGGCCA CTCTGCCAAG AGGTGAATAT CACAGAATAA AGAGTAAATA CCTAGATCAG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_001260091.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247020.1 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant G, mRNA.
    Price & Availability↑
    CloneID OFa08254
    Clone ID Related Accession (Same CDS sequence) NM_001260091.1
    Accession Version NM_001260091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGTGTGCTT AGATCTCAAG TTAGTTATAC CTATAGCGAA 
    ACGGTCGAGA AGAAGAAAGT CCGCCTGAAC AGCAGCAACA GCAGCGGACT GGGAGCTCCG 
    TGGCGGCACA GCATGTCCCG GGATCTGGGC AGCGACATGG AATCGATGTT CTCGCGCACG 
    GGGGCCACTC TGCCAAGAGG TGAATATCAC AGAATAAAGA GTAAATACCT AGATCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247020.1
    CDS233..3832
    Translation

    Target ORF information:

    RefSeq Version NM_001260091.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001260091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGTGTGCTT AGATCTCAAG TTAGTTATAC CTATAGCGAA 
    ACGGTCGAGA AGAAGAAAGT CCGCCTGAAC AGCAGCAACA GCAGCGGACT GGGAGCTCCG 
    TGGCGGCACA GCATGTCCCG GGATCTGGGC AGCGACATGG AATCGATGTT CTCGCGCACG 
    GGGGCCACTC TGCCAAGAGG TGAATATCAC AGAATAAAGA GTAAATACCT AGATCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_206470.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996192.1 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant C, mRNA.
    Price & Availability↑
    CloneID OFa04448
    Clone ID Related Accession (Same CDS sequence) NM_206470.1 , NM_141734.2 , NM_169337.2
    Accession Version NM_206470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGGTGAATA TCACAGAATA 
    AAGAGTAAAT ACCTAGATCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996192.1
    CDS438..4721
    Translation

    Target ORF information:

    RefSeq Version NM_206470.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_206470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGGTGAATA TCACAGAATA 
    AAGAGTAAAT ACCTAGATCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_141734.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649991.2 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant A, mRNA.
    Price & Availability↑
    CloneID OFa04448
    Clone ID Related Accession (Same CDS sequence) NM_206470.1 , NM_141734.2 , NM_169337.2
    Accession Version NM_141734.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Mar 18, 2004 this sequence version replaced NM_141734.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGGTGAATA TCACAGAATA 
    AAGAGTAAAT ACCTAGATCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_649991.2
    CDS233..4516
    Translation

    Target ORF information:

    RefSeq Version NM_141734.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_141734.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGGTGAATA TCACAGAATA 
    AAGAGTAAAT ACCTAGATCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_169337.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731469.2 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant B, mRNA.
    Price & Availability↑
    CloneID OFa04448
    Clone ID Related Accession (Same CDS sequence) NM_206470.1 , NM_141734.2 , NM_169337.2
    Accession Version NM_169337.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Mar 18, 2004 this sequence version replaced NM_169337.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGGTGAATA TCACAGAATA 
    AAGAGTAAAT ACCTAGATCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731469.2
    CDS721..5004
    Translation

    Target ORF information:

    RefSeq Version NM_169337.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_169337.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGGTGAATA TCACAGAATA 
    AAGAGTAAAT ACCTAGATCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_001260090.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247019.1 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant F, mRNA.
    Price & Availability↑
    CloneID OFa08252
    Clone ID Related Accession (Same CDS sequence) NM_001260090.1
    Accession Version NM_001260090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGTTGGTTT AACTACTTTA 
    ACTACTACTA CCACGCCCAC AAAGCATGTT ATAACCATTA ACCAGAGTCC GTTCAGAACC 
    GCCAACAAAT CGCATCCGGG CCATTACGCC ACGGCCACGA CCACGGCCAA AACGGTGGCC 
    GTAGCCGTTG CCGGAGTACC GAAGCAGCTA ACAATAACCA CTACGACCAC CACCAACAGC 
    AATTGCAGCA GTAACAACGC AAACGCCAAC GGCAGGAGCA GACCGCCAAC AATCATCCCG 
    GCCATAACCA CAACCACACC CCAGCTGGCC AAAACCACTA GCAGTAGCAC CACCGCCACC 
    ATCACCGCCA CCGCAACCGA AGGCCCAAGG AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247019.1
    CDS721..5319
    Translation

    Target ORF information:

    RefSeq Version NM_001260090.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001260090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGCCGCCT GAACAGCAGC 
    AACAGCAGCG GACTGGGAGC TCCGTGGCGG CACAGCATGT CCCGGGATCT GGGCAGCGAC 
    ATGGAATCGA TGTTCTCGCG CACGGGGGCC ACTCTGCCAA GAGTTGGTTT AACTACTTTA 
    ACTACTACTA CCACGCCCAC AAAGCATGTT ATAACCATTA ACCAGAGTCC GTTCAGAACC 
    GCCAACAAAT CGCATCCGGG CCATTACGCC ACGGCCACGA CCACGGCCAA AACGGTGGCC 
    GTAGCCGTTG CCGGAGTACC GAAGCAGCTA ACAATAACCA CTACGACCAC CACCAACAGC 
    AATTGCAGCA GTAACAACGC AAACGCCAAC GGCAGGAGCA GACCGCCAAC AATCATCCCG 
    GCCATAACCA CAACCACACC CCAGCTGGCC AAAACCACTA GCAGTAGCAC CACCGCCACC 
    ATCACCGCCA CCGCAACCGA AGGCCCAAGG AAGCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_001275528.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262457.1 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant H, mRNA.
    Price & Availability↑
    CloneID OFa08253
    Clone ID Related Accession (Same CDS sequence) NM_001275528.1
    Accession Version NM_001275528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGTGTGCT TAGATCTCAA 
    GTTAGTTATA CCTATAGCGA AACGGTCGAG AAGAAGAAAG TCCGCCTGAA CAGCAGCAAC 
    AGCAGCGGAC TGGGAGCTCC GTGGCGGCAC AGCATGTCCC GGGATCTGGG CAGCGACATG 
    GAATCGATGT TCTCGCGCAC GGGGGCCACT CTGCCAAGAG TTGGTTTAAC TACTTTAACT 
    ACTACTACCA CGCCCACAAA GCATGTTATA ACCATTAACC AGAGTCCGTT CAGAACCGCC 
    AACAAATCGC ATCCGGGCCA TTACGCCACG GCCACGACCA CGGCCAAAAC GGTGGCCGTA 
    GCCGTTGCCG GAGTACCGAA GCAGCTAACA ATAACCACTA CGACCACCAC CAACAGCAAT 
    TGCAGCAGTA ACAACGCAAA CGCCAACGGC AGGAGCAGAC CGCCAACAAT CATCCCGGCC 
    ATAACCACAA CCACACCCCA GCTGGCCAAA ACCACTAGCA GTAGCACCAC CGCCACCATC 
    ACCGCCACCG CAACCGAAGG CCCAAGGAAG CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262457.1
    CDS271..4926
    Translation

    Target ORF information:

    RefSeq Version NM_001275528.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001275528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGTGA TACGCAGGCC AACACCGTTT TATGGCATCG GCAACGGCTT TGGCAATGGC 
    ATGGGTGGTG CTGTTGGAGC AAGTGGTGCG ACAGTGAACA ATGCACCCAA ACGCGCACAG 
    AGTGGCAGCA GTTTTATGGG CTATTCGGGG GCCACATCAC CGGGCACCGC AGCAACATCG 
    CCACCGATTT CTGGACCCCA AACGGCACCG CCAGTGCTAA TATCACCGAA TGCATTGGCT 
    GTGGCCAAGC AAACGATACA ACAACGGATT TTCGGAGGTG GACTGCCCAG TCCCAATAAT 
    CAGCCAGCAA ATCGAAGGCC AGCCACCTGG CAACCGCAGG CATCCTACAG CACGGCCAGC 
    ATTTTTCAGC CGCCACAAAG TCAGAGTAGT CCCTTCAAGA TGCTGCAGCA GCAATATCAG 
    CAGCGTTGTC AGGAGGACCA GAAGCAAAGT TGCCCATTCA CGCCGCCTCC ATCGCCACAA 
    AATGCCGCCA GCCACGCCTA CGCCGCCCAA GCCAACAGCA ACAACTATGG AGGCAACAGC 
    AACAGCGGTG CCAGCGGCGG CAGCAGCAGC AACAGCAGAT GCAACAGCAG CAACATAGGC 
    AACTGCAACA CGATGCCTTA TGTCAATTAC AATGTCAATT CGCGCACGAG ACCATTTAAT 
    CATAATCAAG CTCAAGACAC ACTCAATACC AATGCCAATA CCAGTGCCAG TGCTACATGT 
    GCCTTGCCTG TGGCTATGTG GCTGAAATCT TCACCGGGTG CAGAAGGACC GCCAACTCCT 
    CCCGGAGCGG TGAAGACATC GACGGCTTCG ATTACCCTGA AATCGGCCAC CCTGCCGCGT 
    CGCAAGATTA ACGCCAAGGC GGAGGTTCAG CTGGACATTA AGCCGCGAAT CCCGGAACAA 
    GGAGCCCCAC CCCAGCCGGC CATGCGATTC AGCACCGAGA TGCAACATCC GGTGGCCGAT 
    CTGCGCAGTG CCCCGCCCCG CGAGGGCCCC ATCCCCTACA GTCCCATCAA GACAACGCTG 
    CAGGCGAGGG CCACTAGTCT GGAGCCCAAG GAGCACGTCA TCACCATCCA GCGACCGCCC 
    ACGCAGCATG CCTATGGGCG AACCAGTTCC AACACAACCA CGCGTTCCGG CTCGCTGTCA 
    CGGCAGTCGA CGGTGGAATC CGAGTCTGAG GGGACAACCA CGGCCACGAA CATGTCGCAG 
    GCCACCATTA CGAGCACCTC GCAGCCCATC AAGAAGAGTC CGCGGGAGTT TATCATCCCG 
    ATTGCCGTCG AGGGAGGCGG CTTCATTACG CCCCGGGAAC GCAGCGTGGA GCCATCGGAA 
    TCGAGCCACA CCACCAGCAG CCGCTCCACG TTCACCCGCC TGCGTCCTTC CCGTCGCATT 
    GGCTCACTGT TAAGCGAGGC AGGCTTCGAT GAGGGCTCGC CATTCCAAAA GATGCGCACC 
    ACGTCGATAA CCCGGGATGG CGTCAGCGGA GGAGCCGAGG AGGAGGCGCG CTTCACTCCG 
    CACCGACTCA GAAGCTCAAG ACCTGTCAAG AAAATTAGTC AAGACAACGA TTCGCAAAGC 
    TCCAACGAGG AGGACGATGA CGACGATGAT GGCTTCGAGA TACTCACGGC GGAGAATCTG 
    TTCTCGACTT TGCTGCAGCG GGTGCGGGCC CTGACGAATC GCCTGAATGT CAACAGTGAC 
    CTGACGGCCG GATTCCCCAG CCATTCTAGT CGCCTGCTCA CGGACATTTC GCGGCAGGCC 
    CAAAGTCACA GTCCATTCTG GAGCCCGGGC AGTCCCTTTG CCAGTGTGCT TAGATCTCAA 
    GTTAGTTATA CCTATAGCGA AACGGTCGAG AAGAAGAAAG TCCGCCTGAA CAGCAGCAAC 
    AGCAGCGGAC TGGGAGCTCC GTGGCGGCAC AGCATGTCCC GGGATCTGGG CAGCGACATG 
    GAATCGATGT TCTCGCGCAC GGGGGCCACT CTGCCAAGAG TTGGTTTAAC TACTTTAACT 
    ACTACTACCA CGCCCACAAA GCATGTTATA ACCATTAACC AGAGTCCGTT CAGAACCGCC 
    AACAAATCGC ATCCGGGCCA TTACGCCACG GCCACGACCA CGGCCAAAAC GGTGGCCGTA 
    GCCGTTGCCG GAGTACCGAA GCAGCTAACA ATAACCACTA CGACCACCAC CAACAGCAAT 
    TGCAGCAGTA ACAACGCAAA CGCCAACGGC AGGAGCAGAC CGCCAACAAT CATCCCGGCC 
    ATAACCACAA CCACACCCCA GCTGGCCAAA ACCACTAGCA GTAGCACCAC CGCCACCATC 
    ACCGCCACCG CAACCGAAGG CCCAAGGAAG CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_141736.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649993.2 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant E, mRNA.
    Price & Availability↑
    CloneID OFa04449
    Clone ID Related Accession (Same CDS sequence) NM_141736.3
    Accession Version NM_141736.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141736.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGCCGCCTG AACAGCAGCA ACAGCAGCGG ACTGGGAGCT 
    CCGTGGCGGC ACAGCATGTC CCGGGATCTG GGCAGCGACA TGGAATCGAT GTTCTCGCGC 
    ACGGGGGCCA CTCTGCCAAG AGGCCATCAT CAAGGCAAGG GCAAGCCCAG TTCAGCTCCC 
    GCCCAAGTGG AAAAGGGTGC CGCCACGGTG TGTTCAGCAA GCACTGCAAA AAGCGAGGAG 
    CCCTTGGACT TGGCGGATCT GGATCTATCG AGACTACGTC TCAGCAAGAA GGATTTGGAA 
    ACCCTGTCTA GCATAACTCC TGGATTGCCC AAGTGTTTCC AGGAACAGTT ACTTGCAAAA 
    CTGCCACCCA CTCAAGCTCG CAAGCTATCA CGCACCCTAA GCGTTCAGAC CAGTGCACCC 
    AGTAGTGCCT CCACTCCCAA GGTTTACAAG CGCAGTCAAA GCAGTGGAAG GGGTTACCAG 
    CCGGAGCAAC AGCCACAAGA GGAGCTGGCT AAACCACTAA CCACTGACGC ACCCAAGACG 
    ACTTCAGCGA GTACAGCGAT TTACAGGCGA AGCCTAAGTC GTAGTCAGGC ACCCACGCCA 
    AATTCTGGTC AGGATGCCGA CAGCCGAGCA GCCCCAACGC CTAAAAGCCG CAGCAGCAGT 
    GTCTGTCGAG ATGACTATGG GAAATCCTCA CTCTGCAGCA GCAGCACTTA CACCAGCGAC 
    ATTAGCGAAA AGTACAAGTA CTACTCGCCG TATCTGAGCA AGTCCAGCAA TGTAAGTCCA 
    TCGATAGCTT CAAAGGATGC CCCAGAGAAG CGGTACAGTG GTGGACTAGG ACGTCCCCCG 
    AGTGGCTGTC TCTCGCCTCC ACCGCAATTG CCGCAAGTTG CTGCCACTGA GGGCACAAGT 
    TCCTTGAGGG CTGGACGTTC ATCGCAGCGT CGGATTTCCC GCTTCCTGCG TCCCGACTTC 
    TTTGACGAAC CTAGGGATCG GGAAACCCAG AGCATGCTCA GGGAAATACG AGAAAAGTCC 
    ATCGATCGCC AGGATCAGGA GCAGACCCAG CCACAGGACT TGAGACGTCG CAAGCATAGT 
    TTGCCTAATG TGGAGCAAGC GGAGCAACCT GCTGAAACGG CACCCATTAG ATCCTCCATG 
    CGCCATTCAC GCAACAAAAG CATGGAGTTG TTTACAGAAG CCGAATCAAA TGGCCAAGTT 
    AGGGATACTG TGAAGACATC AACATCCCGG CAACGAAGTG TTTCGAAGGC TCGAGAATTG 
    GAAACGGAAC TGGACAAACA CGAACTGGCG GATAAAATCC TCCAGGAGCT GCAGCTACTG 
    TCCGCTGTAA GAAGCCAACA TGAGCAGACT CAGGAGTCGG AGAAAGCAGA AGAGACATCC 
    ACCATTAAAA AGATAAAGAA GTTGAAACCC ACGGAATCCA ACGAGCCAGA GGCAACGAAA 
    ATCGCGTCAA CAACTACCTC AAGTACAACC ACTTTGGTTA GGAAGGTTAA GAAGGTTGTT 
    AAGAAAACCG ACGAGGCCCC CAAGACAACT GGATCTGAAA ATGCAGACCT CGCTAGCTCT 
    GCACCAAAGG AAACGAAATT AAAGCGTCCC AAATCCTATC CCATGAAAGA TCTTGGATTA 
    AGTCTTAAAT CATCAACCGA TCTACCAGAA CCGACTACAT CGCTTCTACC CACTAAATTA 
    CCCAGCAAAC TCCCTTCAGG GAACACCAAT GAATCCGCAG TAGAACCAGC TCCTCGCGAG 
    AGTCGCCTGA TGAGGCCGAA AAGCTACCCG GCCACCAAAC TAGCCACTCC CAAGGAACTG 
    CGCAAAGTTA CACGAAGTGG AGTTGCCATA CCCACAATAA CAGAAGCTAT ACCCACTCCC 
    TCGGCTATGC CAACGTCGAC TAGCTCCAAG TCCACTTCGG AGGAGAAATC GACGTCGGCC 
    ACACCAACCG GAACTATGTC TGCCGTAACT GATGCCAAGG ATATAGTATC CTCTTCCAGC 
    GACTCGCGGC CCACGACCAT TAAGAAAATC ATTAAGGTGT CGAAGAAGTC CAAGCTAATG 
    CCACCCACGC CAGTGACACC AACCACTCCA ACCACGACTG CTACCACCAC TGCAAGCACC 
    ACACCAGTAG AAAGCTCTAA GGAGTCTAGT CCGGTCATTA AGCCCAAGGA GAAGTCACCG 
    GAAAAGAAAC CCAGCAAGGG TTTGCTTTAC GCTTTGGGAC AGAAGTTTGA GAAACTACGA 
    GACTCCGCCA TGAGCAAGGA GAAAAAGGCT GGTTCTACTG GAGCAGCAGC ATCTTTGGCC 
    TGCACCCAAA AGCAAGGGTC TCCCGAAGAA CGCAGCTCTC CAGAGAAGTA TAAGAAGAAC 
    GTTTCGGATA CAGAGAAGTC CCAACAAACT GGACTCTCCG ACGACAAACG GGTGGATAAG 
    CGATCCCGGT TTGACACCAT GCTACGCTCT TTAAGAGAGA GATCCGTACC CAGATCACAA 
    CCCAATGGAG GCGTGAGTCC CAAGCGAGCT GCCAGTGTGG AGGAGCTGCA CGCCGGATGC 
    CCAGAGGGGC AGGGAAAACA AGGCGGAGCA GTTAACAAGA TGTTCGGCGG TTTGTTGCGT 
    CGATTTGACA GGGAAAGCAG TGAACAACGC CGGGTGAGAA GCTCCCGATC CACCAGCAAC 
    ATAGAGCGAC AGGTTCGCGA AGAACAGGAT CAGCTGCTGG ACGCCTCGCT ACCAACATCG 
    CCAATCTATC AGAATGTGGT TAAGAGCACA AAAAGCAAAG TCTCTTCAAC GGCAAATGGC 
    GAAGAGAATT GCAATTGCGA AACAGCTTGC CCAGATTGCA GGCAAAACAA TGGACAGAAC 
    GTAACCACCA GCGGTGCTCC AACCAACACA ACAATGCCAA GCAGCAAGGA GAAGCGAAAG 
    GGTCTGATGC TTGATCTGGC CAGCGCAAAT GTGTCCGCCA GTGGTGCCCC CAGCTCGGTG 
    ACCACCGCCA CCACAACTAC CACAAGTGGA AGCAGCTATC GGGGATCTAA AACCAATCCG 
    GCTTTGATCA ATGGAAATGG AGGACTGGGA ATGGGCATCT ACTCGAATCT GCCACCCTAT 
    CCGGGAGCCA TGAAAAGTGC CAGCTCCAAC AACAGCTCCA GCCAGCAGTC CATGGAGAAC 
    GCCACCAACA ATAACTCAAC AAATACCAAT AATAATTGCA GTTCGCAAAC TTCTGGCTAT 
    AGAACCTCAT CGGCACATGA GATTAATCGG AATAATCCGC TCTTGACGCC ATCCTTCGAA 
    AACATTGCCA ACTACTCCTC GGACTCAAGG AGCTACCAAG ATGACTGCGC CTCCACCTCG 
    ACTTTCCTAT CGCCCACCGA AGAGCCGGAG TTGTATTTTG ACAATTGGTC CATATGCTCC 
    GAGGACAACT ATATGTTGCA CGCGACGCCC TCGCCCACAG TTTCCCGGTT GTCAAGAGCT 
    TCATTGTCCT CGCCCACTCG TTGCTCCGAG AGCTCTGATC CCAACGAGAG TGTGATCGAC 
    AGGATTAAAC GGCGTAGCTT CTACAGTCGA TTCAATGAGC AGAAAAAACC ACGACGCACT 
    AGCAGCATAG TCGGACCATC GGCTGTGAGG GACTACTACC GGGAGCAACA AGCGGCAGTT 
    AAGGCGCGCT CCAGTAACAA ACTGCATGCT CCGGACATGG ATCCACCACC AAGAGCTCAT 
    TCACCGGACA TAGCCCAGCA GTTCTTTAGA CCCCTGAAAC TGAGTCCCGT GGGAACGGAG 
    CTAAAGCCCC CTGTTTACCG CTCCACTTTG GACTACTCTG CATCATCGGC GGGGGCCACC 
    AGCAAGCCAA GAAAGAGTCT GAATGACATT AGGAATACCT CGCCCTCTTT CCTCAGCAAA 
    CGTTACGAGA CCACCGATTA CAGTTCGGTG CCAATGCGAT ACAAGAGCTC CTCCAGTTCG 
    AGTCCAAGTA ATGGAGCCAT TGCCAGCGGC TATTACAACA CCTACAATCC AAAGCGTAGA 
    TCTTCGTATA CGCTCAATGG TAGTCTGCCT ACAGCGACCA GTGGAAGCAG TGCCGCAGGG 
    AGCAGCCACC TGGATGGATA CGCCACCCTG GGCAGGAGAT CCCTTCGTCC ATACGACCAT 
    CGAACGATGT CCCTGCTGGA GCCACCGACC TCGAGCAGCA GTCGTAGTGG TGAAGGGGTT 
    TCCTACCAAA GGCGTGAAGC AAGGACACCG GTTAGAGACT ACACATCCAG TATTTCGCGA 
    TCTGGGTCGC GTTACCGCAC TTCATCGGCC ACTCGCAGTC CAACAAACAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_649993.2
    CDS233..7846
    Translation

    Target ORF information:

    RefSeq Version NM_141736.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_141736.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGCCGCCTG AACAGCAGCA ACAGCAGCGG ACTGGGAGCT 
    CCGTGGCGGC ACAGCATGTC CCGGGATCTG GGCAGCGACA TGGAATCGAT GTTCTCGCGC 
    ACGGGGGCCA CTCTGCCAAG AGGCCATCAT CAAGGCAAGG GCAAGCCCAG TTCAGCTCCC 
    GCCCAAGTGG AAAAGGGTGC CGCCACGGTG TGTTCAGCAA GCACTGCAAA AAGCGAGGAG 
    CCCTTGGACT TGGCGGATCT GGATCTATCG AGACTACGTC TCAGCAAGAA GGATTTGGAA 
    ACCCTGTCTA GCATAACTCC TGGATTGCCC AAGTGTTTCC AGGAACAGTT ACTTGCAAAA 
    CTGCCACCCA CTCAAGCTCG CAAGCTATCA CGCACCCTAA GCGTTCAGAC CAGTGCACCC 
    AGTAGTGCCT CCACTCCCAA GGTTTACAAG CGCAGTCAAA GCAGTGGAAG GGGTTACCAG 
    CCGGAGCAAC AGCCACAAGA GGAGCTGGCT AAACCACTAA CCACTGACGC ACCCAAGACG 
    ACTTCAGCGA GTACAGCGAT TTACAGGCGA AGCCTAAGTC GTAGTCAGGC ACCCACGCCA 
    AATTCTGGTC AGGATGCCGA CAGCCGAGCA GCCCCAACGC CTAAAAGCCG CAGCAGCAGT 
    GTCTGTCGAG ATGACTATGG GAAATCCTCA CTCTGCAGCA GCAGCACTTA CACCAGCGAC 
    ATTAGCGAAA AGTACAAGTA CTACTCGCCG TATCTGAGCA AGTCCAGCAA TGTAAGTCCA 
    TCGATAGCTT CAAAGGATGC CCCAGAGAAG CGGTACAGTG GTGGACTAGG ACGTCCCCCG 
    AGTGGCTGTC TCTCGCCTCC ACCGCAATTG CCGCAAGTTG CTGCCACTGA GGGCACAAGT 
    TCCTTGAGGG CTGGACGTTC ATCGCAGCGT CGGATTTCCC GCTTCCTGCG TCCCGACTTC 
    TTTGACGAAC CTAGGGATCG GGAAACCCAG AGCATGCTCA GGGAAATACG AGAAAAGTCC 
    ATCGATCGCC AGGATCAGGA GCAGACCCAG CCACAGGACT TGAGACGTCG CAAGCATAGT 
    TTGCCTAATG TGGAGCAAGC GGAGCAACCT GCTGAAACGG CACCCATTAG ATCCTCCATG 
    CGCCATTCAC GCAACAAAAG CATGGAGTTG TTTACAGAAG CCGAATCAAA TGGCCAAGTT 
    AGGGATACTG TGAAGACATC AACATCCCGG CAACGAAGTG TTTCGAAGGC TCGAGAATTG 
    GAAACGGAAC TGGACAAACA CGAACTGGCG GATAAAATCC TCCAGGAGCT GCAGCTACTG 
    TCCGCTGTAA GAAGCCAACA TGAGCAGACT CAGGAGTCGG AGAAAGCAGA AGAGACATCC 
    ACCATTAAAA AGATAAAGAA GTTGAAACCC ACGGAATCCA ACGAGCCAGA GGCAACGAAA 
    ATCGCGTCAA CAACTACCTC AAGTACAACC ACTTTGGTTA GGAAGGTTAA GAAGGTTGTT 
    AAGAAAACCG ACGAGGCCCC CAAGACAACT GGATCTGAAA ATGCAGACCT CGCTAGCTCT 
    GCACCAAAGG AAACGAAATT AAAGCGTCCC AAATCCTATC CCATGAAAGA TCTTGGATTA 
    AGTCTTAAAT CATCAACCGA TCTACCAGAA CCGACTACAT CGCTTCTACC CACTAAATTA 
    CCCAGCAAAC TCCCTTCAGG GAACACCAAT GAATCCGCAG TAGAACCAGC TCCTCGCGAG 
    AGTCGCCTGA TGAGGCCGAA AAGCTACCCG GCCACCAAAC TAGCCACTCC CAAGGAACTG 
    CGCAAAGTTA CACGAAGTGG AGTTGCCATA CCCACAATAA CAGAAGCTAT ACCCACTCCC 
    TCGGCTATGC CAACGTCGAC TAGCTCCAAG TCCACTTCGG AGGAGAAATC GACGTCGGCC 
    ACACCAACCG GAACTATGTC TGCCGTAACT GATGCCAAGG ATATAGTATC CTCTTCCAGC 
    GACTCGCGGC CCACGACCAT TAAGAAAATC ATTAAGGTGT CGAAGAAGTC CAAGCTAATG 
    CCACCCACGC CAGTGACACC AACCACTCCA ACCACGACTG CTACCACCAC TGCAAGCACC 
    ACACCAGTAG AAAGCTCTAA GGAGTCTAGT CCGGTCATTA AGCCCAAGGA GAAGTCACCG 
    GAAAAGAAAC CCAGCAAGGG TTTGCTTTAC GCTTTGGGAC AGAAGTTTGA GAAACTACGA 
    GACTCCGCCA TGAGCAAGGA GAAAAAGGCT GGTTCTACTG GAGCAGCAGC ATCTTTGGCC 
    TGCACCCAAA AGCAAGGGTC TCCCGAAGAA CGCAGCTCTC CAGAGAAGTA TAAGAAGAAC 
    GTTTCGGATA CAGAGAAGTC CCAACAAACT GGACTCTCCG ACGACAAACG GGTGGATAAG 
    CGATCCCGGT TTGACACCAT GCTACGCTCT TTAAGAGAGA GATCCGTACC CAGATCACAA 
    CCCAATGGAG GCGTGAGTCC CAAGCGAGCT GCCAGTGTGG AGGAGCTGCA CGCCGGATGC 
    CCAGAGGGGC AGGGAAAACA AGGCGGAGCA GTTAACAAGA TGTTCGGCGG TTTGTTGCGT 
    CGATTTGACA GGGAAAGCAG TGAACAACGC CGGGTGAGAA GCTCCCGATC CACCAGCAAC 
    ATAGAGCGAC AGGTTCGCGA AGAACAGGAT CAGCTGCTGG ACGCCTCGCT ACCAACATCG 
    CCAATCTATC AGAATGTGGT TAAGAGCACA AAAAGCAAAG TCTCTTCAAC GGCAAATGGC 
    GAAGAGAATT GCAATTGCGA AACAGCTTGC CCAGATTGCA GGCAAAACAA TGGACAGAAC 
    GTAACCACCA GCGGTGCTCC AACCAACACA ACAATGCCAA GCAGCAAGGA GAAGCGAAAG 
    GGTCTGATGC TTGATCTGGC CAGCGCAAAT GTGTCCGCCA GTGGTGCCCC CAGCTCGGTG 
    ACCACCGCCA CCACAACTAC CACAAGTGGA AGCAGCTATC GGGGATCTAA AACCAATCCG 
    GCTTTGATCA ATGGAAATGG AGGACTGGGA ATGGGCATCT ACTCGAATCT GCCACCCTAT 
    CCGGGAGCCA TGAAAAGTGC CAGCTCCAAC AACAGCTCCA GCCAGCAGTC CATGGAGAAC 
    GCCACCAACA ATAACTCAAC AAATACCAAT AATAATTGCA GTTCGCAAAC TTCTGGCTAT 
    AGAACCTCAT CGGCACATGA GATTAATCGG AATAATCCGC TCTTGACGCC ATCCTTCGAA 
    AACATTGCCA ACTACTCCTC GGACTCAAGG AGCTACCAAG ATGACTGCGC CTCCACCTCG 
    ACTTTCCTAT CGCCCACCGA AGAGCCGGAG TTGTATTTTG ACAATTGGTC CATATGCTCC 
    GAGGACAACT ATATGTTGCA CGCGACGCCC TCGCCCACAG TTTCCCGGTT GTCAAGAGCT 
    TCATTGTCCT CGCCCACTCG TTGCTCCGAG AGCTCTGATC CCAACGAGAG TGTGATCGAC 
    AGGATTAAAC GGCGTAGCTT CTACAGTCGA TTCAATGAGC AGAAAAAACC ACGACGCACT 
    AGCAGCATAG TCGGACCATC GGCTGTGAGG GACTACTACC GGGAGCAACA AGCGGCAGTT 
    AAGGCGCGCT CCAGTAACAA ACTGCATGCT CCGGACATGG ATCCACCACC AAGAGCTCAT 
    TCACCGGACA TAGCCCAGCA GTTCTTTAGA CCCCTGAAAC TGAGTCCCGT GGGAACGGAG 
    CTAAAGCCCC CTGTTTACCG CTCCACTTTG GACTACTCTG CATCATCGGC GGGGGCCACC 
    AGCAAGCCAA GAAAGAGTCT GAATGACATT AGGAATACCT CGCCCTCTTT CCTCAGCAAA 
    CGTTACGAGA CCACCGATTA CAGTTCGGTG CCAATGCGAT ACAAGAGCTC CTCCAGTTCG 
    AGTCCAAGTA ATGGAGCCAT TGCCAGCGGC TATTACAACA CCTACAATCC AAAGCGTAGA 
    TCTTCGTATA CGCTCAATGG TAGTCTGCCT ACAGCGACCA GTGGAAGCAG TGCCGCAGGG 
    AGCAGCCACC TGGATGGATA CGCCACCCTG GGCAGGAGAT CCCTTCGTCC ATACGACCAT 
    CGAACGATGT CCCTGCTGGA GCCACCGACC TCGAGCAGCA GTCGTAGTGG TGAAGGGGTT 
    TCCTACCAAA GGCGTGAAGC AAGGACACCG GTTAGAGACT ACACATCCAG TATTTCGCGA 
    TCTGGGTCGC GTTACCGCAC TTCATCGGCC ACTCGCAGTC CAACAAACAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nuak1 ( NM_001275529.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262458.1 Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant I, mRNA.
    Price & Availability↑
    CloneID OFa08251
    Clone ID Related Accession (Same CDS sequence) NM_001275529.1
    Accession Version NM_001275529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product Nuak1 ortholog, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGTGTGCTT AGATCTCAAG TTAGTTATAC CTATAGCGAA 
    ACGGTCGAGA AGAAGAAAGT CCGCCTGAAC AGCAGCAACA GCAGCGGACT GGGAGCTCCG 
    TGGCGGCACA GCATGTCCCG GGATCTGGGC AGCGACATGG AATCGATGTT CTCGCGCACG 
    GGGGCCACTC TGCCAAGAGG CCATCATCAA GGCAAGGGCA AGCCCAGTTC AGCTCCCGCC 
    CAAGTGGAAA AGGGTGCCGC CACGGTGTGT TCAGCAAGCA CTGCAAAAAG CGAGGAGCCC 
    TTGGACTTGG CGGATCTGGA TCTATCGAGA CTACGTCTCA GCAAGAAGGA TTTGGAAACC 
    CTGTCTAGCA TAACTCCTGG ATTGCCCAAG TGTTTCCAGG AACAGTTACT TGCAAAACTG 
    CCACCCACTC AAGCTCGCAA GCTATCACGC ACCCTAAGCG TTCAGACCAG TGCACCCAGT 
    AGTGCCTCCA CTCCCAAGGT TTACAAGCGC AGTCAAAGCA GTGGAAGGGG TTACCAGCCG 
    GAGCAACAGC CACAAGAGGA GCTGGCTAAA CCACTAACCA CTGACGCACC CAAGACGACT 
    TCAGCGAGTA CAGCGATTTA CAGGCGAAGC CTAAGTCGTA GTCAGGCACC CACGCCAAAT 
    TCTGGTCAGG ATGCCGACAG CCGAGCAGCC CCAACGCCTA AAAGCCGCAG CAGCAGTGTC 
    TGTCGAGATG ACTATGGGAA ATCCTCACTC TGCAGCAGCA GCACTTACAC CAGCGACATT 
    AGCGAAAAGT ACAAGTACTA CTCGCCGTAT CTGAGCAAGT CCAGCAATGT AAGTCCATCG 
    ATAGCTTCAA AGGATGCCCC AGAGAAGCGG TACAGTGGTG GACTAGGACG TCCCCCGAGT 
    GGCTGTCTCT CGCCTCCACC GCAATTGCCG CAAGTTGCTG CCACTGAGGG CACAAGTTCC 
    TTGAGGGCTG GACGTTCATC GCAGCGTCGG ATTTCCCGCT TCCTGCGTCC CGACTTCTTT 
    GACGAACCTA GGGATCGGGA AACCCAGAGC ATGCTCAGGG AAATACGAGA AAAGTCCATC 
    GATCGCCAGG ATCAGGAGCA GACCCAGCCA CAGGACTTGA GACGTCGCAA GCATAGTTTG 
    CCTAATGTGG AGCAAGCGGA GCAACCTGCT GAAACGGCAC CCATTAGATC CTCCATGCGC 
    CATTCACGCA ACAAAAGCAT GGAGTTGTTT ACAGAAGCCG AATCAAATGG CCAAGTTAGG 
    GATACTGTGA AGACATCAAC ATCCCGGCAA CGAAGTGTTT CGAAGGCTCG AGAATTGGAA 
    ACGGAACTGG ACAAACACGA ACTGGCGGAT AAAATCCTCC AGGAGCTGCA GCTACTGTCC 
    GCTGTAAGAA GCCAACATGA GCAGACTCAG GAGTCGGAGA AAGCAGAAGA GACATCCACC 
    ATTAAAAAGA TAAAGAAGTT GAAACCCACG GAATCCAACG AGCCAGAGGC AACGAAAATC 
    GCGTCAACAA CTACCTCAAG TACAACCACT TTGGTTAGGA AGGTTAAGAA GGTTGTTAAG 
    AAAACCGACG AGGCCCCCAA GACAACTGGA TCTGAAAATG CAGACCTCGC TAGCTCTGCA 
    CCAAAGGAAA CGAAATTAAA GCGTCCCAAA TCCTATCCCA TGAAAGATCT TGGATTAAGT 
    CTTAAATCAT CAACCGATCT ACCAGAACCG ACTACATCGC TTCTACCCAC TAAATTACCC 
    AGCAAACTCC CTTCAGGGAA CACCAATGAA TCCGCAGTAG AACCAGCTCC TCGCGAGAGT 
    CGCCTGATGA GGCCGAAAAG CTACCCGGCC ACCAAACTAG CCACTCCCAA GGAACTGCGC 
    AAAGTTACAC GAAGTGGAGT TGCCATACCC ACAATAACAG AAGCTATACC CACTCCCTCG 
    GCTATGCCAA CGTCGACTAG CTCCAAGTCC ACTTCGGAGG AGAAATCGAC GTCGGCCACA 
    CCAACCGGAA CTATGTCTGC CGTAACTGAT GCCAAGGATA TAGTATCCTC TTCCAGCGAC 
    TCGCGGCCCA CGACCATTAA GAAAATCATT AAGGTGTCGA AGAAGTCCAA GCTAATGCCA 
    CCCACGCCAG TGACACCAAC CACTCCAACC ACGACTGCTA CCACCACTGC AAGCACCACA 
    CCAGTAGAAA GCTCTAAGGA GTCTAGTCCG GTCATTAAGC CCAAGGAGAA GTCACCGGAA 
    AAGAAACCCA GCAAGGGTTT GCTTTACGCT TTGGGACAGA AGTTTGAGAA ACTACGAGAC 
    TCCGCCATGA GCAAGGAGAA AAAGGCTGGT TCTACTGGAG CAGCAGCATC TTTGGCCTGC 
    ACCCAAAAGC AAGGGTCTCC CGAAGAACGC AGCTCTCCAG AGAAGTATAA GAAGAACGTT 
    TCGGATACAG AGAAGTCCCA ACAAACTGGA CTCTCCGACG ACAAACGGGT GGATAAGCGA 
    TCCCGGTTTG ACACCATGCT ACGCTCTTTA AGAGAGAGAT CCGTACCCAG ATCACAACCC 
    AATGGAGGCG TGAGTCCCAA GCGAGCTGCC AGTGTGGAGG AGCTGCACGC CGGATGCCCA 
    GAGGGGCAGG GAAAACAAGG CGGAGCAGTT AACAAGATGT TCGGCGGTTT GTTGCGTCGA 
    TTTGACAGGG AAAGCAGTGA ACAACGCCGG GTGAGAAGCT CCCGATCCAC CAGCAACATA 
    GAGCGACAGG TTCGCGAAGA ACAGGATCAG CTGCTGGACG CCTCGCTACC AACATCGCCA 
    ATCTATCAGA ATGTGGTTAA GAGCACAAAA AGCAAAGTCT CTTCAACGGC AAATGGCGAA 
    GAGAATTGCA ATTGCGAAAC AGCTTGCCCA GATTGCAGGC AAAACAATGG ACAGAACGTA 
    ACCACCAGCG GTGCTCCAAC CAACACAACA ATGCCAAGCA GCAAGGAGAA GCGAAAGGGT 
    CTGATGCTTG ATCTGGCCAG CGCAAATGTG TCCGCCAGTG GTGCCCCCAG CTCGGTGACC 
    ACCGCCACCA CAACTACCAC AAGTGGAAGC AGCTATCGGG GATCTAAAAC CAATCCGGCT 
    TTGATCAATG GAAATGGAGG ACTGGGAATG GGCATCTACT CGAATCTGCC ACCCTATCCG 
    GGAGCCATGA AAAGTGCCAG CTCCAACAAC AGCTCCAGCC AGCAGTCCAT GGAGAACGCC 
    ACCAACAATA ACTCAACAAA TACCAATAAT AATTGCAGTT CGCAAACTTC TGGCTATAGA 
    ACCTCATCGG CACATGAGAT TAATCGGAAT AATCCGCTCT TGACGCCATC CTTCGAAAAC 
    ATTGCCAACT ACTCCTCGGA CTCAAGGAGC TACCAAGATG ACTGCGCCTC CACCTCGACT 
    TTCCTATCGC CCACCGAAGA GCCGGAGTTG TATTTTGACA ATTGGTCCAT ATGCTCCGAG 
    GACAACTATA TGTTGCACGC GACGCCCTCG CCCACAGTTT CCCGGTTGTC AAGAGCTTCA 
    TTGTCCTCGC CCACTCGTTG CTCCGAGAGC TCTGATCCCA ACGAGAGTGT GATCGACAGG 
    ATTAAACGGC GTAGCTTCTA CAGTCGATTC AATGAGCAGA AAAAACCACG ACGCACTAGC 
    AGCATAGTCG GACCATCGGC TGTGAGGGAC TACTACCGGG AGCAACAAGC GGCAGTTAAG 
    GCGCGCTCCA GTAACAAACT GCATGCTCCG GACATGGATC CACCACCAAG AGCTCATTCA 
    CCGGACATAG CCCAGCAGTT CTTTAGACCC CTGAAACTGA GTCCCGTGGG AACGGAGCTA 
    AAGCCCCCTG TTTACCGCTC CACTTTGGAC TACTCTGCAT CATCGGCGGG GGCCACCAGC 
    AAGCCAAGAA AGAGTCTGAA TGACATTAGG AATACCTCGC CCTCTTTCCT CAGCAAACGT 
    TACGAGACCA CCGATTACAG TTCGGTGCCA ATGCGATACA AGAGCTCCTC CAGTTCGAGT 
    CCAAGTAATG GAGCCATTGC CAGCGGCTAT TACAACACCT ACAATCCAAA GCGTAGATCT 
    TCGTATACGC TCAATGGTAG TCTGCCTACA GCGACCAGTG GAAGCAGTGC CGCAGGGAGC 
    AGCCACCTGG ATGGATACGC CACCCTGGGC AGGAGATCCC TTCGTCCATA CGACCATCGA 
    ACGATGTCCC TGCTGGAGCC ACCGACCTCG AGCAGCAGTC GTAGTGGTGA AGGGGTTTCC 
    TACCAAAGGC GTGAAGCAAG GACACCGGTT AGAGACTACA CATCCAGTAT TTCGCGATCT 
    GGGTCGCGTT ACCGCACTTC ATCGGCCACT CGCAGTCCAA CAAACATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262458.1
    CDS721..8391
    Translation

    Target ORF information:

    RefSeq Version NM_001275529.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster Nuak1 ortholog (Nuak1), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001275529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGTGATAA GCAAACCCGA TGGAACGGCG CCCAATGGAG CGGCGGGAGG AGCGGAAGCC 
    GCTGCCCCAA CTGGCCTTGA TGCCACCGGG AACAGTCTGG CGCATCCTTC GGGAATACCG 
    CAGGATCAGA TAGACAACAT CATGAGCGGC ATCGCCAACA CGGGCAATGT CAAAATGAAC 
    AATCACCGCA AGAAGTTGCG ACAAAGATTT GATATTATTA AGAAACTAGG ACAAGGCACT 
    TACGGCAAGG TGCAGTTAGG TATTAATAAG GAAACCGGCC AGGAGGTGGC TATCAAAACC 
    ATCAAGAAGT GCAAGATCGA GGCCGAGGCG GATTTGGTGC GCATCCGTCG CGAGGTGCAG 
    ATTATGAGCT CAGTGCATCA TCCCAACATC ATCCACATCT ACGAAGTATT TGAGAATCGT 
    GAGAAAATGG TGCTAGTCAT GGAATTTGCC GCTGGCGGCG AGCTCTACGA CTATCTGTCT 
    GAAAGGAAGG TTCTCACCGA GGAGGAGGCG AGACGCATCT TCCGCCAGGT GGCCACCGCC 
    GTCTACTACT GTCACAAGCA CAAGATCTGC CATCGCGATC TCAAGCTGGA GAACATCCTG 
    CTGGACGAGA AGGGCAATGC TAAGATTGCT GATTTTGGGT TGTCGAATGT GTTTGATGAC 
    CAGCGACTGC TGGGCACCTT TTGCGGTTCC CCACTCTATG CCTCGCCGGA AATTGTGGAA 
    GGCACACCAT ACCAGGGACC CGAGGTGGAC TGCTGGTCAC TGGGCGTGCT GCTCTACACG 
    CTGGTCTACG GCTCTATGCC CTTCGATGGG TCCAATTTCA AGCGACTGGT GAAGCAGATC 
    AGCCAGGGTG ATTACTACGA GCCAAGGAAA CCTTCGCGAG CCTCTACTCT CATCCGGGAC 
    ATGCTGACCG TGTGTCCGAG GAAACGGGCC AGTATCGAGC AAATCTGCTC GCACTGGTGG 
    GTCAACGAGA ACGATAATGT GTCCTGTCTG GATCTGGCCG AGGATCTGGC CAATCAGACC 
    CCAGTGCGAT TGGATGTCCT GCTTTCCCTT ACGCCAGCCA CAATCACCGC GGATCAGTTG 
    GTGGTGCCAT CGGCGGAGGG AGCAGCTGCT GCCAAGGCGG CGGCCAATGA ACGCGTTCCT 
    CGCTCGCATT CGGTGGGCTC GATCCGGGAC ATGGGACCGC CGAACACGGA GGCGGAACGT 
    CGCATCCTGG ACATGGTGGC CGCTGGAGGA GAAGCCGCCT TGATGCCCTC ACCCACCAGG 
    ACCATAACCC CCGCCCAGAG TCCGGTGCAG ACGAAGAGGA AACTGCAGCC CACTGTTTCT 
    ACGGAGAATG CAGCCGGAAC TACAGCCAAA AAGAAGGAGA AGCCGGCCAA TAGCTCGTTT 
    GTGATCAGCA AGGATGGTGC ACCACTGACT GAAGCGCCAC CGACCATCAT CGAACCGCAA 
    GCTACGCTCA TGGAGGCGGA GACGATTGCC AATATACCCG AGGAGGTTCC TGTTCCCAGT 
    TACTCCCAGA AAGACATGCA AGCGGTGGGT GATATTTGCG ATCTGCTGAT GGGCACCGCC 
    ACAACTCCAG CCCCTGCACC ACCGGCACCC ACACCTGTTG CTCGCCAGCC CACCAGGGGA 
    AAACTGGATG CCGTTGTTGA AACTCCTGAG GAAAAGGATG CCACCAAAGT GATCAAGAAG 
    TTTGTGAACA AGCACAAGAC CGCGGATCTG GTAAATGCCA TCAATGAAAG TGCCTCCAAG 
    GCCGCAGCGC CGGTGAGCGC AGTGGCCCCT CCACCCTTCG TCCGCAAGTG CAGCTTGCAG 
    GATGAGTCCA CACTGAATAA ATTCAATGCG GAGCGTCGGA AATCGCGAAT CCTAGAAACT 
    GCAGAGAAGT TCCAGCCACC GCCACCAGTT GCTGCAGCAC CTCCCGAAAA ACCCAAGAAG 
    CTCAGCATAC CGGGTGTCAG TGTGGGCAGT TTTAAGAAGG AGTTCGAGAA GAAGGCCACC 
    AATCCACCGG CTGCAGAGGG ACCTACGCCC GGTGAATTAA GGGCTCAGGA GCAGGTTGCA 
    GCAGCTGCAG CGGCCGCCCA GGAAGCCGAA ACCTTAAGCA CCCCGCCACC ATCGCCAGTG 
    GTGGCCCAAT CGCTAGAGGG CAGCGATTCC AAGAACTCTG TGGCCTCTAT TTCGCTGGAC 
    GAGGCTCGCC GCTCCATGGA GAACTCCATT GCCTTGCTGC TCCAGGCACA AAACGAGTCC 
    AGCAAGGAGG TGGACCAGTT GTGTGCCCAA ACGGAGACCA TTGGCGTCAG TGAACCGGCT 
    ACTCAGCAGG AGCGCGAGCG TAAGTTGAAG AACGCCCGCG CCATAATCGG AAATGCCATA 
    CAACCAGCTT CACCGGGTGC AGAAGGACCG CCAACTCCTC CCGGAGCGGT GAAGACATCG 
    ACGGCTTCGA TTACCCTGAA ATCGGCCACC CTGCCGCGTC GCAAGATTAA CGCCAAGGCG 
    GAGGTTCAGC TGGACATTAA GCCGCGAATC CCGGAACAAG GAGCCCCACC CCAGCCGGCC 
    ATGCGATTCA GCACCGAGAT GCAACATCCG GTGGCCGATC TGCGCAGTGC CCCGCCCCGC 
    GAGGGCCCCA TCCCCTACAG TCCCATCAAG ACAACGCTGC AGGCGAGGGC CACTAGTCTG 
    GAGCCCAAGG AGCACGTCAT CACCATCCAG CGACCGCCCA CGCAGCATGC CTATGGGCGA 
    ACCAGTTCCA ACACAACCAC GCGTTCCGGC TCGCTGTCAC GGCAGTCGAC GGTGGAATCC 
    GAGTCTGAGG GGACAACCAC GGCCACGAAC ATGTCGCAGG CCACCATTAC GAGCACCTCG 
    CAGCCCATCA AGAAGAGTCC GCGGGAGTTT ATCATCCCGA TTGCCGTCGA GGGAGGCGGC 
    TTCATTACGC CCCGGGAACG CAGCGTGGAG CCATCGGAAT CGAGCCACAC CACCAGCAGC 
    CGCTCCACGT TCACCCGCCT GCGTCCTTCC CGTCGCATTG GCTCACTGTT AAGCGAGGCA 
    GGCTTCGATG AGGGCTCGCC ATTCCAAAAG ATGCGCACCA CGTCGATAAC CCGGGATGGC 
    GTCAGCGGAG GAGCCGAGGA GGAGGCGCGC TTCACTCCGC ACCGACTCAG AAGCTCAAGA 
    CCTGTCAAGA AAATTAGTCA AGACAACGAT TCGCAAAGCT CCAACGAGGA GGACGATGAC 
    GACGATGATG GCTTCGAGAT ACTCACGGCG GAGAATCTGT TCTCGACTTT GCTGCAGCGG 
    GTGCGGGCCC TGACGAATCG CCTGAATGTC AACAGTGACC TGACGGCCGG ATTCCCCAGC 
    CATTCTAGTC GCCTGCTCAC GGACATTTCG CGGCAGGCCC AAAGTCACAG TCCATTCTGG 
    AGCCCGGGCA GTCCCTTTGC CAGTGTGCTT AGATCTCAAG TTAGTTATAC CTATAGCGAA 
    ACGGTCGAGA AGAAGAAAGT CCGCCTGAAC AGCAGCAACA GCAGCGGACT GGGAGCTCCG 
    TGGCGGCACA GCATGTCCCG GGATCTGGGC AGCGACATGG AATCGATGTT CTCGCGCACG 
    GGGGCCACTC TGCCAAGAGG CCATCATCAA GGCAAGGGCA AGCCCAGTTC AGCTCCCGCC 
    CAAGTGGAAA AGGGTGCCGC CACGGTGTGT TCAGCAAGCA CTGCAAAAAG CGAGGAGCCC 
    TTGGACTTGG CGGATCTGGA TCTATCGAGA CTACGTCTCA GCAAGAAGGA TTTGGAAACC 
    CTGTCTAGCA TAACTCCTGG ATTGCCCAAG TGTTTCCAGG AACAGTTACT TGCAAAACTG 
    CCACCCACTC AAGCTCGCAA GCTATCACGC ACCCTAAGCG TTCAGACCAG TGCACCCAGT 
    AGTGCCTCCA CTCCCAAGGT TTACAAGCGC AGTCAAAGCA GTGGAAGGGG TTACCAGCCG 
    GAGCAACAGC CACAAGAGGA GCTGGCTAAA CCACTAACCA CTGACGCACC CAAGACGACT 
    TCAGCGAGTA CAGCGATTTA CAGGCGAAGC CTAAGTCGTA GTCAGGCACC CACGCCAAAT 
    TCTGGTCAGG ATGCCGACAG CCGAGCAGCC CCAACGCCTA AAAGCCGCAG CAGCAGTGTC 
    TGTCGAGATG ACTATGGGAA ATCCTCACTC TGCAGCAGCA GCACTTACAC CAGCGACATT 
    AGCGAAAAGT ACAAGTACTA CTCGCCGTAT CTGAGCAAGT CCAGCAATGT AAGTCCATCG 
    ATAGCTTCAA AGGATGCCCC AGAGAAGCGG TACAGTGGTG GACTAGGACG TCCCCCGAGT 
    GGCTGTCTCT CGCCTCCACC GCAATTGCCG CAAGTTGCTG CCACTGAGGG CACAAGTTCC 
    TTGAGGGCTG GACGTTCATC GCAGCGTCGG ATTTCCCGCT TCCTGCGTCC CGACTTCTTT 
    GACGAACCTA GGGATCGGGA AACCCAGAGC ATGCTCAGGG AAATACGAGA AAAGTCCATC 
    GATCGCCAGG ATCAGGAGCA GACCCAGCCA CAGGACTTGA GACGTCGCAA GCATAGTTTG 
    CCTAATGTGG AGCAAGCGGA GCAACCTGCT GAAACGGCAC CCATTAGATC CTCCATGCGC 
    CATTCACGCA ACAAAAGCAT GGAGTTGTTT ACAGAAGCCG AATCAAATGG CCAAGTTAGG 
    GATACTGTGA AGACATCAAC ATCCCGGCAA CGAAGTGTTT CGAAGGCTCG AGAATTGGAA 
    ACGGAACTGG ACAAACACGA ACTGGCGGAT AAAATCCTCC AGGAGCTGCA GCTACTGTCC 
    GCTGTAAGAA GCCAACATGA GCAGACTCAG GAGTCGGAGA AAGCAGAAGA GACATCCACC 
    ATTAAAAAGA TAAAGAAGTT GAAACCCACG GAATCCAACG AGCCAGAGGC AACGAAAATC 
    GCGTCAACAA CTACCTCAAG TACAACCACT TTGGTTAGGA AGGTTAAGAA GGTTGTTAAG 
    AAAACCGACG AGGCCCCCAA GACAACTGGA TCTGAAAATG CAGACCTCGC TAGCTCTGCA 
    CCAAAGGAAA CGAAATTAAA GCGTCCCAAA TCCTATCCCA TGAAAGATCT TGGATTAAGT 
    CTTAAATCAT CAACCGATCT ACCAGAACCG ACTACATCGC TTCTACCCAC TAAATTACCC 
    AGCAAACTCC CTTCAGGGAA CACCAATGAA TCCGCAGTAG AACCAGCTCC TCGCGAGAGT 
    CGCCTGATGA GGCCGAAAAG CTACCCGGCC ACCAAACTAG CCACTCCCAA GGAACTGCGC 
    AAAGTTACAC GAAGTGGAGT TGCCATACCC ACAATAACAG AAGCTATACC CACTCCCTCG 
    GCTATGCCAA CGTCGACTAG CTCCAAGTCC ACTTCGGAGG AGAAATCGAC GTCGGCCACA 
    CCAACCGGAA CTATGTCTGC CGTAACTGAT GCCAAGGATA TAGTATCCTC TTCCAGCGAC 
    TCGCGGCCCA CGACCATTAA GAAAATCATT AAGGTGTCGA AGAAGTCCAA GCTAATGCCA 
    CCCACGCCAG TGACACCAAC CACTCCAACC ACGACTGCTA CCACCACTGC AAGCACCACA 
    CCAGTAGAAA GCTCTAAGGA GTCTAGTCCG GTCATTAAGC CCAAGGAGAA GTCACCGGAA 
    AAGAAACCCA GCAAGGGTTT GCTTTACGCT TTGGGACAGA AGTTTGAGAA ACTACGAGAC 
    TCCGCCATGA GCAAGGAGAA AAAGGCTGGT TCTACTGGAG CAGCAGCATC TTTGGCCTGC 
    ACCCAAAAGC AAGGGTCTCC CGAAGAACGC AGCTCTCCAG AGAAGTATAA GAAGAACGTT 
    TCGGATACAG AGAAGTCCCA ACAAACTGGA CTCTCCGACG ACAAACGGGT GGATAAGCGA 
    TCCCGGTTTG ACACCATGCT ACGCTCTTTA AGAGAGAGAT CCGTACCCAG ATCACAACCC 
    AATGGAGGCG TGAGTCCCAA GCGAGCTGCC AGTGTGGAGG AGCTGCACGC CGGATGCCCA 
    GAGGGGCAGG GAAAACAAGG CGGAGCAGTT AACAAGATGT TCGGCGGTTT GTTGCGTCGA 
    TTTGACAGGG AAAGCAGTGA ACAACGCCGG GTGAGAAGCT CCCGATCCAC CAGCAACATA 
    GAGCGACAGG TTCGCGAAGA ACAGGATCAG CTGCTGGACG CCTCGCTACC AACATCGCCA 
    ATCTATCAGA ATGTGGTTAA GAGCACAAAA AGCAAAGTCT CTTCAACGGC AAATGGCGAA 
    GAGAATTGCA ATTGCGAAAC AGCTTGCCCA GATTGCAGGC AAAACAATGG ACAGAACGTA 
    ACCACCAGCG GTGCTCCAAC CAACACAACA ATGCCAAGCA GCAAGGAGAA GCGAAAGGGT 
    CTGATGCTTG ATCTGGCCAG CGCAAATGTG TCCGCCAGTG GTGCCCCCAG CTCGGTGACC 
    ACCGCCACCA CAACTACCAC AAGTGGAAGC AGCTATCGGG GATCTAAAAC CAATCCGGCT 
    TTGATCAATG GAAATGGAGG ACTGGGAATG GGCATCTACT CGAATCTGCC ACCCTATCCG 
    GGAGCCATGA AAAGTGCCAG CTCCAACAAC AGCTCCAGCC AGCAGTCCAT GGAGAACGCC 
    ACCAACAATA ACTCAACAAA TACCAATAAT AATTGCAGTT CGCAAACTTC TGGCTATAGA 
    ACCTCATCGG CACATGAGAT TAATCGGAAT AATCCGCTCT TGACGCCATC CTTCGAAAAC 
    ATTGCCAACT ACTCCTCGGA CTCAAGGAGC TACCAAGATG ACTGCGCCTC CACCTCGACT 
    TTCCTATCGC CCACCGAAGA GCCGGAGTTG TATTTTGACA ATTGGTCCAT ATGCTCCGAG 
    GACAACTATA TGTTGCACGC GACGCCCTCG CCCACAGTTT CCCGGTTGTC AAGAGCTTCA 
    TTGTCCTCGC CCACTCGTTG CTCCGAGAGC TCTGATCCCA ACGAGAGTGT GATCGACAGG 
    ATTAAACGGC GTAGCTTCTA CAGTCGATTC AATGAGCAGA AAAAACCACG ACGCACTAGC 
    AGCATAGTCG GACCATCGGC TGTGAGGGAC TACTACCGGG AGCAACAAGC GGCAGTTAAG 
    GCGCGCTCCA GTAACAAACT GCATGCTCCG GACATGGATC CACCACCAAG AGCTCATTCA 
    CCGGACATAG CCCAGCAGTT CTTTAGACCC CTGAAACTGA GTCCCGTGGG AACGGAGCTA 
    AAGCCCCCTG TTTACCGCTC CACTTTGGAC TACTCTGCAT CATCGGCGGG GGCCACCAGC 
    AAGCCAAGAA AGAGTCTGAA TGACATTAGG AATACCTCGC CCTCTTTCCT CAGCAAACGT 
    TACGAGACCA CCGATTACAG TTCGGTGCCA ATGCGATACA AGAGCTCCTC CAGTTCGAGT 
    CCAAGTAATG GAGCCATTGC CAGCGGCTAT TACAACACCT ACAATCCAAA GCGTAGATCT 
    TCGTATACGC TCAATGGTAG TCTGCCTACA GCGACCAGTG GAAGCAGTGC CGCAGGGAGC 
    AGCCACCTGG ATGGATACGC CACCCTGGGC AGGAGATCCC TTCGTCCATA CGACCATCGA 
    ACGATGTCCC TGCTGGAGCC ACCGACCTCG AGCAGCAGTC GTAGTGGTGA AGGGGTTTCC 
    TACCAAAGGC GTGAAGCAAG GACACCGGTT AGAGACTACA CATCCAGTAT TTCGCGATCT 
    GGGTCGCGTT ACCGCACTTC ATCGGCCACT CGCAGTCCAA CAAACATTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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