CG6254 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6254
Entrez Gene ID	41244
Full Name	CG6254 gene product from transcript CG6254-RA
Synonyms	Dmel\CG6254
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85F15-85F15

mRNA and Protein(s)

mRNA	Protein	Name
NM_141726.3	NP_649983.2	uncharacterized protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG17568	NP_609951.2
Drosophila melanogaster (fruit fly)	CG6254	NP_649983.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009010	XP_319758.4
Drosophila melanogaster (fruit fly)	CG10366	NP_609995.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6254 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6254 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04434	NM_141726.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG6254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6254 ( NM_141726.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649983.2 Drosophila melanogaster uncharacterized protein (CG6254), mRNA.

Price & Availability↑

CloneID	OFa04434
Clone ID Related Accession (Same CDS sequence)	NM_141726.3
Accession Version	NM_141726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141726.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGATA TTCTCGCAGA AACACAAGCC GCGAACAGAG ATTCAACAAT TTGGCACCAA 
TGGTGTCGCC TGTGCTCCAA GGAGCATCCG ACAAATCAAA ATGTATTCTT CAGGGAGGCG 
AAACAAAACT CCTGGGCGAG CACAATGGCC ATGACCATTG GCAAGTACTT TTGGGTCGAT 
ATCAAAAGGG AGGACGAGCT GAGCAATTTT CTGTGCTCCG AATGCTTCAC ATTGATGGAC 
TGCCTCATCG AGTTTTCGGA GAGGGTTCGC AAAGTGCAGA TCCTTTTCAG CAAATTGCAG 
TTGTTACAGT CTAATAATTT GATGGATTAT GAGAAAATTC GAGAGGACTG CGGTGTGGCA 
ACCGATGGAT GGAAGCATAT AATGCTCGGA GCCGTAGACG AAAGTCTTCC CCAGAATGAC 
GAAGTATTCA TCAGCGAGGA ACAACCAATA GTCTGCCAAA CAGAAACATC CAGTAAAATG 
AAAAGTGAAA TCCTAATGAT ACCAAATTTT TTGGTGGAAA AACAATGTCT TCAGGAAAAA 
CAAGACTTTC CTAAAGAGGA AGATGTTATT GAAGAAGAAA TGCAAATTTC TGGGGTGGAA 
GAGGAAATCC TAGAAGAAGA TGTGGAAGAA GATCTTGCCG AGGAAGAGGT TGAAACTGTG 
GAGGAAGAGA TTGACACTGT GGGAGAAGAG GTTGAAGCTG TGGAGGAAGA GTTGGAAACC 
CAAGACGCTA CAGATTGCTT AGTGGAGGAA GTGGAGCACA TGACAGAGGA TCGCTATATC 
GAGGAGTCGC AGATTATAGA AGAAAGCCAA GTTTCAGATT TCAACATGGA AACATATGAA 
ATAGTACAAC ACAATCCGCA AAAAGAACCC GTAGAAACAA AAGACACCGT GGAATCTATA 
GAATCAAACG AGGATACCCA GGAAGATATC TCCCGAGAGC ATGTAACCGA TGAGGAGGAT 
GAAATATCAG AGGTACCAGC CATGTATAAG TGCAATATTT GCAAAAAGCC GTATAAGAAA 
CCCAAAGCCT ACAAGCGTCA CATGGAGGAG GTTCACAACA CTGTGGCCGA TGATCTGCCA 
CAGTTGGAGT GCAATCAGTG CAAACTATGT TTTCCAACAG TCGCCCAACT GCATGCCCAC 
CACCGAACCC ATGTACGTGC CAAACCAAAG ACAGATAACT GCTGTCCACA CTGCGAAAAG 
CGCTTCACCA CCTCGGGAAC ACTGAAGCGA CACATAGAAG GCATCCACAA CCAAATAAAG 
CCCTATGTGT GCGACCTCTG TGGCAAAAGC TTCAATTACA TTACTGGACT GAAGGATCAC 
AAACTTGTGC ACACAGATGA GTGCCCCTTC GAGTGTCCCG TGTGCAAGCG AGGCTTTAAA 
AACAATGCGA GGTTAAAGAT CCATTTAGAT ACACACAGTG CCGAAATCTA CGAGTGCACT 
GTATGTGGCT TGAAGTTAAA AACCCGACGC ACATTCAACA AACACAAATT GGTGCATTCA 
GATACACGGC AATTTAAGTG TGAAGTCTGC GGATCGGCGT TCAAGCGAAG TAAGACACTG 
AAGGCACATC TCATCCTTCA CACCGGCATC CGGCCATATA AGTGCAATTT TTGCGGAAGA 
GACTTCGCCA ATGGCTCCAA CTGTCGATCG CACAAACGGC AGGCACACCC CAAGGAACTG 
GCCGAAGAGG AAGCTCGCGG AGTGACACGA TCCACACTTC TTCCAATGCT GGACGAACTG 
ACAATTGCGT CCAAGCTTTT AAAAACACCC GCAGGTCCTT GCAAAGTAAA GGGTAGTAGG 
CCAAAAGCTG CGCCCAAAGA TCAAGATAAT GGCAATGAAT CTCCAACGAA GGACACCGAT 
GGTGCCATCC TCTACGAGCT GGTCGAGGAG CTTGATTACT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649983.2
CDS	71..1975
Translation	MSDILAETQAANRDSTIWHQWCRLCSKEHPTNQNVFFREAKQNSWASTMAMTIGKYFWVDIKREDELSNFLCSECFTLMDCLIEFSERVRKVQILFSKLQLLQSNNLMDYEKIREDCGVATDGWKHIMLGAVDESLPQNDEVFISEEQPIVCQTETSSKMKSEILMIPNFLVEKQCLQEKQDFPKEEDVIEEEMQISGVEEEILEEDVEEDLAEEEVETVEEEIDTVGEEVEAVEEELETQDATDCLVEEVEHMTEDRYIEESQIIEESQVSDFNMETYEIVQHNPQKEPVETKDTVESIESNEDTQEDISREHVTDEEDEISEVPAMYKCNICKKPYKKPKAYKRHMEEVHNTVADDLPQLECNQCKLCFPTVAQLHAHHRTHVRAKPKTDNCCPHCEKRFTTSGTLKRHIEGIHNQIKPYVCDLCGKSFNYITGLKDHKLVHTDECPFECPVCKRGFKNNARLKIHLDTHSAEIYECTVCGLKLKTRRTFNKHKLVHSDTRQFKCEVCGSAFKRSKTLKAHLILHTGIRPYKCNFCGRDFANGSNCRSHKRQAHPKELAEEEARGVTRSTLLPMLDELTIASKLLKTPAGPCKVKGSRPKAAPKDQDNGNESPTKDTDGAILYELVEELDYS

Target ORF information:

RefSeq Version	NM_141726.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG6254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141726.3

ORF Insert Sequence:

ATGTCGGATA TTCTCGCAGA AACACAAGCC GCGAACAGAG ATTCAACAAT TTGGCACCAA 
TGGTGTCGCC TGTGCTCCAA GGAGCATCCG ACAAATCAAA ATGTATTCTT CAGGGAGGCG 
AAACAAAACT CCTGGGCGAG CACAATGGCC ATGACCATTG GCAAGTACTT TTGGGTCGAT 
ATCAAAAGGG AGGACGAGCT GAGCAATTTT CTGTGCTCCG AATGCTTCAC ATTGATGGAC 
TGCCTCATCG AGTTTTCGGA GAGGGTTCGC AAAGTGCAGA TCCTTTTCAG CAAATTGCAG 
TTGTTACAGT CTAATAATTT GATGGATTAT GAGAAAATTC GAGAGGACTG CGGTGTGGCA 
ACCGATGGAT GGAAGCATAT AATGCTCGGA GCCGTAGACG AAAGTCTTCC CCAGAATGAC 
GAAGTATTCA TCAGCGAGGA ACAACCAATA GTCTGCCAAA CAGAAACATC CAGTAAAATG 
AAAAGTGAAA TCCTAATGAT ACCAAATTTT TTGGTGGAAA AACAATGTCT TCAGGAAAAA 
CAAGACTTTC CTAAAGAGGA AGATGTTATT GAAGAAGAAA TGCAAATTTC TGGGGTGGAA 
GAGGAAATCC TAGAAGAAGA TGTGGAAGAA GATCTTGCCG AGGAAGAGGT TGAAACTGTG 
GAGGAAGAGA TTGACACTGT GGGAGAAGAG GTTGAAGCTG TGGAGGAAGA GTTGGAAACC 
CAAGACGCTA CAGATTGCTT AGTGGAGGAA GTGGAGCACA TGACAGAGGA TCGCTATATC 
GAGGAGTCGC AGATTATAGA AGAAAGCCAA GTTTCAGATT TCAACATGGA AACATATGAA 
ATAGTACAAC ACAATCCGCA AAAAGAACCC GTAGAAACAA AAGACACCGT GGAATCTATA 
GAATCAAACG AGGATACCCA GGAAGATATC TCCCGAGAGC ATGTAACCGA TGAGGAGGAT 
GAAATATCAG AGGTACCAGC CATGTATAAG TGCAATATTT GCAAAAAGCC GTATAAGAAA 
CCCAAAGCCT ACAAGCGTCA CATGGAGGAG GTTCACAACA CTGTGGCCGA TGATCTGCCA 
CAGTTGGAGT GCAATCAGTG CAAACTATGT TTTCCAACAG TCGCCCAACT GCATGCCCAC 
CACCGAACCC ATGTACGTGC CAAACCAAAG ACAGATAACT GCTGTCCACA CTGCGAAAAG 
CGCTTCACCA CCTCGGGAAC ACTGAAGCGA CACATAGAAG GCATCCACAA CCAAATAAAG 
CCCTATGTGT GCGACCTCTG TGGCAAAAGC TTCAATTACA TTACTGGACT GAAGGATCAC 
AAACTTGTGC ACACAGATGA GTGCCCCTTC GAGTGTCCCG TGTGCAAGCG AGGCTTTAAA 
AACAATGCGA GGTTAAAGAT CCATTTAGAT ACACACAGTG CCGAAATCTA CGAGTGCACT 
GTATGTGGCT TGAAGTTAAA AACCCGACGC ACATTCAACA AACACAAATT GGTGCATTCA 
GATACACGGC AATTTAAGTG TGAAGTCTGC GGATCGGCGT TCAAGCGAAG TAAGACACTG 
AAGGCACATC TCATCCTTCA CACCGGCATC CGGCCATATA AGTGCAATTT TTGCGGAAGA 
GACTTCGCCA ATGGCTCCAA CTGTCGATCG CACAAACGGC AGGCACACCC CAAGGAACTG 
GCCGAAGAGG AAGCTCGCGG AGTGACACGA TCCACACTTC TTCCAATGCT GGACGAACTG 
ACAATTGCGT CCAAGCTTTT AAAAACACCC GCAGGTCCTT GCAAAGTAAA GGGTAGTAGG 
CCAAAAGCTG CGCCCAAAGA TCAAGATAAT GGCAATGAAT CTCCAACGAA GGACACCGAT 
GGTGCCATCC TCTACGAGCT GGTCGAGGAG CTTGATTACT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6254 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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