Dh44 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dh44
Entrez Gene ID	41170
Full Name	Diuretic hormone 44
Synonyms	CG8348,CRF,DH,DH 44,DH44,DH[[44]],Dh,Dmel\CG8348,Drm-DH,Drome-DH[[44]],drome-DH44
General protein information	Preferred Names Diuretic hormone 44 Names CG8348 gene product from transcript CG8348-RA CG8348-PA CG8348-PC CRF-like diuretic hormone CRF-like peptide Dh44-PA Dh44-PC diuretic hormone diuretic hormone 44
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85E2-85E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_141665.3	NP_649922.2	diuretic hormone 44, isoform A
NM_001104255.3	NP_001097725.2	diuretic hormone 44, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DH44 Kinin neuropeptide modulates desiccation and starvation tolerance in Drosophila melanogaster.
Title: The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster.

The Dh44/DH44 receptor system directs the detection and consumption of nutritive sugars through a positive feedback loop.
Title: Nutrient Sensor in the Brain Directs the Action of the Brain-Gut Axis in Drosophila.

not upregulated by cold exposure
Title: A comparison of Frost expression among species and life stages of Drosophila.

Results describe Dh, the gene that encodes a CRF-like peptide (Drome-DH(44)) in Drosophila melanogaster.
Title: The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP.

The following Dh44 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dh44 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04361	NM_001104255.3 Latest version!	Drosophila melanogaster diuretic hormone 44, transcript variant C (Dh44), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04361	NM_141665.3 Latest version!	Drosophila melanogaster diuretic hormone 44, transcript variant A (Dh44), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dh44 ( NM_141665.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649922.2 Drosophila melanogaster diuretic hormone 44, transcript variant A (Dh44), mRNA.

Price & Availability↑

CloneID	OFa04361
Clone ID Related Accession (Same CDS sequence)	NM_141665.3 , NM_001104255.3
Accession Version	NM_141665.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diuretic hormone 44, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_141665.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAAAG CCACAGCGTG GTTTTGTCCG GTGTTATTAA CTTTACTGTG TGCCACAAGG 
CTCGTCTGCA CGGCCCAAAG AGGAGCAGTT GGAGCAGGAG GAGCTGCAGG AGGATCGGGA 
GCGGCAGCGG GTGGAGCGGA GGTCGGCGGA AGCGGCCGCA CAAACGGATA TCCTCTGGAC 
TACCCGGATG GAACACGGAA CTCACAGGAT GACTTTCTGC TGGCCAAGAG GAACAAACCA 
TCGCTGTCCA TTGTCAATCC GCTGGATGTG CTGCGCCAGC GTCTGCTCCT GGAAATCGCA 
CGCAGGCAAA TGAAGGAGAA CTCCCGCCAG GTCGAACTGA ATCGCGCCAT CCTGAAGAAC 
GTGGGCAAGA GGGTCGTGCT GCGAGGAGGA GGAGGAGGCG GAGGATCGGG AGCAGGAGGA 
TTGGCTCCCA AGGTGAGCAG GCGCTACCGC CAGCAGTGGC CAGTGGAGCG GGAACTGGAG 
CGGGAAAGGC AGAGGGAGAG GGAAAGGGAA AGGGATGCAG TTCGTGAGGA GCAGCTGGAC 
CGTCAACAGC TGCTGCCGTG GAAGCATTTC CCCAGCCAAC TGTGGAGCTA CGGATGGGCC 
CTGTCGCCGT ACAAGGAGTC GTCACAGTTA CAGTTTGCGG ACTCCCAGCA ATCAGCATCG 
ACTGGACCAC AATCCCAGGC ACTCCCAAAG CAGTTGCAGT TGCTCAGCTA TGCGAAGAAA 
CCACTGGACG TGGCCGGAAT GAGTCTGGCC AGGCATCGGG TCAGCGGAAA TGAAGCCAAC 
GAAACGAATC ATGAGAACGA CGATGGCAAT GGGGCCAGTA AAAACCCGGC GAGATATGTG 
GATGACGGCG ACAATGAGGG CGAGGATAGC TACAATGACG TGGGAACAGA AGGTGTGGGA 
CTCGGCCTGG GAATGGGTGT GGGATTGGGT CTGGAACGGT TCGAGGTGCT GGAGGACAAG 
CCCAACTGGG CCAATGAGGA ACCAAACGAG TTGGTGGTGG TTAATGCAAA TGACCGCGTG 
CCCTGGTCCT TTCCCTACCG CTTTCACAAA AGTCAGCATA ACGTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649922.2
CDS	32..1102
Translation	MMKATAWFCPVLLTLLCATRLVCTAQRGAVGAGGAAGGSGAAAGGAEVGGSGRTNGYPLDYPDGTRNSQDDFLLAKRNKPSLSIVNPLDVLRQRLLLEIARRQMKENSRQVELNRAILKNVGKRVVLRGGGGGGGSGAGGLAPKVSRRYRQQWPVERELERERQRERERERDAVREEQLDRQQLLPWKHFPSQLWSYGWALSPYKESSQLQFADSQQSASTGPQSQALPKQLQLLSYAKKPLDVAGMSLARHRVSGNEANETNHENDDGNGASKNPARYVDDGDNEGEDSYNDVGTEGVGLGLGMGVGLGLERFEVLEDKPNWANEEPNELVVVNANDRVPWSFPYRFHKSQHNVN

Target ORF information:

RefSeq Version	NM_141665.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diuretic hormone 44, transcript variant A (Dh44), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141665.3

ORF Insert Sequence:

ATGATGAAAG CCACAGCGTG GTTTTGTCCG GTGTTATTAA CTTTACTGTG TGCCACAAGG 
CTCGTCTGCA CGGCCCAAAG AGGAGCAGTT GGAGCAGGAG GAGCTGCAGG AGGATCGGGA 
GCGGCAGCGG GTGGAGCGGA GGTCGGCGGA AGCGGCCGCA CAAACGGATA TCCTCTGGAC 
TACCCGGATG GAACACGGAA CTCACAGGAT GACTTTCTGC TGGCCAAGAG GAACAAACCA 
TCGCTGTCCA TTGTCAATCC GCTGGATGTG CTGCGCCAGC GTCTGCTCCT GGAAATCGCA 
CGCAGGCAAA TGAAGGAGAA CTCCCGCCAG GTCGAACTGA ATCGCGCCAT CCTGAAGAAC 
GTGGGCAAGA GGGTCGTGCT GCGAGGAGGA GGAGGAGGCG GAGGATCGGG AGCAGGAGGA 
TTGGCTCCCA AGGTGAGCAG GCGCTACCGC CAGCAGTGGC CAGTGGAGCG GGAACTGGAG 
CGGGAAAGGC AGAGGGAGAG GGAAAGGGAA AGGGATGCAG TTCGTGAGGA GCAGCTGGAC 
CGTCAACAGC TGCTGCCGTG GAAGCATTTC CCCAGCCAAC TGTGGAGCTA CGGATGGGCC 
CTGTCGCCGT ACAAGGAGTC GTCACAGTTA CAGTTTGCGG ACTCCCAGCA ATCAGCATCG 
ACTGGACCAC AATCCCAGGC ACTCCCAAAG CAGTTGCAGT TGCTCAGCTA TGCGAAGAAA 
CCACTGGACG TGGCCGGAAT GAGTCTGGCC AGGCATCGGG TCAGCGGAAA TGAAGCCAAC 
GAAACGAATC ATGAGAACGA CGATGGCAAT GGGGCCAGTA AAAACCCGGC GAGATATGTG 
GATGACGGCG ACAATGAGGG CGAGGATAGC TACAATGACG TGGGAACAGA AGGTGTGGGA 
CTCGGCCTGG GAATGGGTGT GGGATTGGGT CTGGAACGGT TCGAGGTGCT GGAGGACAAG 
CCCAACTGGG CCAATGAGGA ACCAAACGAG TTGGTGGTGG TTAATGCAAA TGACCGCGTG 
CCCTGGTCCT TTCCCTACCG CTTTCACAAA AGTCAGCATA ACGTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dh44 ( NM_001104255.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097725.2 Drosophila melanogaster diuretic hormone 44, transcript variant C (Dh44), mRNA.

Price & Availability↑

CloneID	OFa04361
Clone ID Related Accession (Same CDS sequence)	NM_141665.3 , NM_001104255.3
Accession Version	NM_001104255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	diuretic hormone 44, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001104255.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAAAG CCACAGCGTG GTTTTGTCCG GTGTTATTAA CTTTACTGTG TGCCACAAGG 
CTCGTCTGCA CGGCCCAAAG AGGAGCAGTT GGAGCAGGAG GAGCTGCAGG AGGATCGGGA 
GCGGCAGCGG GTGGAGCGGA GGTCGGCGGA AGCGGCCGCA CAAACGGATA TCCTCTGGAC 
TACCCGGATG GAACACGGAA CTCACAGGAT GACTTTCTGC TGGCCAAGAG GAACAAACCA 
TCGCTGTCCA TTGTCAATCC GCTGGATGTG CTGCGCCAGC GTCTGCTCCT GGAAATCGCA 
CGCAGGCAAA TGAAGGAGAA CTCCCGCCAG GTCGAACTGA ATCGCGCCAT CCTGAAGAAC 
GTGGGCAAGA GGGTCGTGCT GCGAGGAGGA GGAGGAGGCG GAGGATCGGG AGCAGGAGGA 
TTGGCTCCCA AGGTGAGCAG GCGCTACCGC CAGCAGTGGC CAGTGGAGCG GGAACTGGAG 
CGGGAAAGGC AGAGGGAGAG GGAAAGGGAA AGGGATGCAG TTCGTGAGGA GCAGCTGGAC 
CGTCAACAGC TGCTGCCGTG GAAGCATTTC CCCAGCCAAC TGTGGAGCTA CGGATGGGCC 
CTGTCGCCGT ACAAGGAGTC GTCACAGTTA CAGTTTGCGG ACTCCCAGCA ATCAGCATCG 
ACTGGACCAC AATCCCAGGC ACTCCCAAAG CAGTTGCAGT TGCTCAGCTA TGCGAAGAAA 
CCACTGGACG TGGCCGGAAT GAGTCTGGCC AGGCATCGGG TCAGCGGAAA TGAAGCCAAC 
GAAACGAATC ATGAGAACGA CGATGGCAAT GGGGCCAGTA AAAACCCGGC GAGATATGTG 
GATGACGGCG ACAATGAGGG CGAGGATAGC TACAATGACG TGGGAACAGA AGGTGTGGGA 
CTCGGCCTGG GAATGGGTGT GGGATTGGGT CTGGAACGGT TCGAGGTGCT GGAGGACAAG 
CCCAACTGGG CCAATGAGGA ACCAAACGAG TTGGTGGTGG TTAATGCAAA TGACCGCGTG 
CCCTGGTCCT TTCCCTACCG CTTTCACAAA AGTCAGCATA ACGTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097725.2
CDS	319..1389
Translation	MMKATAWFCPVLLTLLCATRLVCTAQRGAVGAGGAAGGSGAAAGGAEVGGSGRTNGYPLDYPDGTRNSQDDFLLAKRNKPSLSIVNPLDVLRQRLLLEIARRQMKENSRQVELNRAILKNVGKRVVLRGGGGGGGSGAGGLAPKVSRRYRQQWPVERELERERQRERERERDAVREEQLDRQQLLPWKHFPSQLWSYGWALSPYKESSQLQFADSQQSASTGPQSQALPKQLQLLSYAKKPLDVAGMSLARHRVSGNEANETNHENDDGNGASKNPARYVDDGDNEGEDSYNDVGTEGVGLGLGMGVGLGLERFEVLEDKPNWANEEPNELVVVNANDRVPWSFPYRFHKSQHNVN

Target ORF information:

RefSeq Version	NM_001104255.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster diuretic hormone 44, transcript variant C (Dh44), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104255.3

ORF Insert Sequence:

ATGATGAAAG CCACAGCGTG GTTTTGTCCG GTGTTATTAA CTTTACTGTG TGCCACAAGG 
CTCGTCTGCA CGGCCCAAAG AGGAGCAGTT GGAGCAGGAG GAGCTGCAGG AGGATCGGGA 
GCGGCAGCGG GTGGAGCGGA GGTCGGCGGA AGCGGCCGCA CAAACGGATA TCCTCTGGAC 
TACCCGGATG GAACACGGAA CTCACAGGAT GACTTTCTGC TGGCCAAGAG GAACAAACCA 
TCGCTGTCCA TTGTCAATCC GCTGGATGTG CTGCGCCAGC GTCTGCTCCT GGAAATCGCA 
CGCAGGCAAA TGAAGGAGAA CTCCCGCCAG GTCGAACTGA ATCGCGCCAT CCTGAAGAAC 
GTGGGCAAGA GGGTCGTGCT GCGAGGAGGA GGAGGAGGCG GAGGATCGGG AGCAGGAGGA 
TTGGCTCCCA AGGTGAGCAG GCGCTACCGC CAGCAGTGGC CAGTGGAGCG GGAACTGGAG 
CGGGAAAGGC AGAGGGAGAG GGAAAGGGAA AGGGATGCAG TTCGTGAGGA GCAGCTGGAC 
CGTCAACAGC TGCTGCCGTG GAAGCATTTC CCCAGCCAAC TGTGGAGCTA CGGATGGGCC 
CTGTCGCCGT ACAAGGAGTC GTCACAGTTA CAGTTTGCGG ACTCCCAGCA ATCAGCATCG 
ACTGGACCAC AATCCCAGGC ACTCCCAAAG CAGTTGCAGT TGCTCAGCTA TGCGAAGAAA 
CCACTGGACG TGGCCGGAAT GAGTCTGGCC AGGCATCGGG TCAGCGGAAA TGAAGCCAAC 
GAAACGAATC ATGAGAACGA CGATGGCAAT GGGGCCAGTA AAAACCCGGC GAGATATGTG 
GATGACGGCG ACAATGAGGG CGAGGATAGC TACAATGACG TGGGAACAGA AGGTGTGGGA 
CTCGGCCTGG GAATGGGTGT GGGATTGGGT CTGGAACGGT TCGAGGTGCT GGAGGACAAG 
CCCAACTGGG CCAATGAGGA ACCAAACGAG TTGGTGGTGG TTAATGCAAA TGACCGCGTG 
CCCTGGTCCT TTCCCTACCG CTTTCACAAA AGTCAGCATA ACGTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dh44 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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