pyd cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following pyd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pyd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04284 NM_169246.3
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa04286 NM_001260072.2
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa04286 NM_001104241.2
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa04287 NM_176426.4
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa04282 NM_057349.7
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa07909 NM_001144556.2
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa04283 NM_170646.6
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa04285 NM_169245.3
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OFa04288 NM_001104242.3
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa07908 NM_001260069.2
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OFa07910 NM_001260071.2
Latest version!
Drosophila melanogaster polychaetoid (pyd), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa04284
    Clone ID Related Accession (Same CDS sequence) NM_169246.3
    Accession Version NM_169246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169246.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGTCGGAATA ACTCCGTAAC GCAAGCGGAT
    TATACAAAGC TACCGAAGAC TGCGCCACAT GGAGTAGTTC CTCCGCCGAA TGTGAGCAAT
    GGCCAGGGCC AGATGAATGG CAGCGGAACG CCGAGCAGCA ATGGCAGTGG ACCCTTCAAG
    CCGGTGCCAC CACCCAAACC CAAAAACTAT AGGCCACCGG TGCAAAGTGG TGGTAGCAGT
    GGCAGCGGAG GCACAACTCC TTGGGAGAAT GGGGACTCTG GTTCGCCCCG TTCTCCGAAT
    GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCATTACG GCCAGCAGGC GACCCCGGGT
    TCACCCAGCA ATGGCCACAT GCAGCCACCA CCACCGCAGC AGCAGCAGCC CACCTACGGC
    GGAAGTAATG GCAACTATGG CCAGGCACCA CCACCACAAT CCTATCCGCA GGCCAATGGC
    TACAATGGGA ATGGGCATCA CTACAACGGG GGCAGCGGCA CAGGACCCTA TATTGCACCA
    CATCGCGGCA TGCCACCGCC AATAGGAAAC CTGCCGCCTC ACACGCCCGA ACGTCATGCT
    CTTGACTTGG CCGGAAGTCG GGAGCAGCGC GGTTCGGCCT TCGAACTGTA TCGTAAACCG
    CAAATCGGAG CTACCGCCGG GCACCATCAC AACATGAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731292.2
    CDS326..4207
    Translation

    Target ORF information:

    RefSeq Version NM_169246.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGTCGGAATA ACTCCGTAAC GCAAGCGGAT
    TATACAAAGC TACCGAAGAC TGCGCCACAT GGAGTAGTTC CTCCGCCGAA TGTGAGCAAT
    GGCCAGGGCC AGATGAATGG CAGCGGAACG CCGAGCAGCA ATGGCAGTGG ACCCTTCAAG
    CCGGTGCCAC CACCCAAACC CAAAAACTAT AGGCCACCGG TGCAAAGTGG TGGTAGCAGT
    GGCAGCGGAG GCACAACTCC TTGGGAGAAT GGGGACTCTG GTTCGCCCCG TTCTCCGAAT
    GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCATTACG GCCAGCAGGC GACCCCGGGT
    TCACCCAGCA ATGGCCACAT GCAGCCACCA CCACCGCAGC AGCAGCAGCC CACCTACGGC
    GGAAGTAATG GCAACTATGG CCAGGCACCA CCACCACAAT CCTATCCGCA GGCCAATGGC
    TACAATGGGA ATGGGCATCA CTACAACGGG GGCAGCGGCA CAGGACCCTA TATTGCACCA
    CATCGCGGCA TGCCACCGCC AATAGGAAAC CTGCCGCCTC ACACGCCCGA ACGTCATGCT
    CTTGACTTGG CCGGAAGTCG GGAGCAGCGC GGTTCGGCCT TCGAACTGTA TCGTAAACCG
    CAAATCGGAG CTACCGCCGG GCACCATCAC AACATGAGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04286
    Clone ID Related Accession (Same CDS sequence) NM_001104241.2 , NM_001260072.2
    Accession Version NM_001104241.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104241.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGACGCTATC ATTTACATGC GCCCCATTTG
    AAGCACTTGC ATATGATGCA CTCGCTCTCA CCTTCGTCCT CGTTGTCGCT CTCACCTTCG
    CCCTCGTCCT TGCCCCTTCA CATGTCCTGC GGCCTTCAGC TGACACAGCA GCAGCTGCTA
    CGCCTCTCCA CCGGCGGCTT CGATCGCCGA CCCATTGCTG CACCACGTCT GCATGCCCTG
    ATGGCACCAC TGCCACCGCC AGCTCTGATG TCCAGTGCCT CGGCCTACGA TACACTCGAC
    TCTGGTTCGC CCCGTTCTCC GAATGGCTTC TATTATCCAC CAACGCCTTC GCATCATCAT
    TACGGCCAGC AGGCGACCCC GGGTTCACCC AGCAATGGCC ACATGCAGCC ACCACCACCG
    CAGCAGCAGC AGCCCACCTA CGGCGGAAGT AATGGCAACT ATGGCCAGGC ACCACCACCA
    CAATCCTATC CGCAGGCCAA TGGCTACAAT GGGAATGGGC ATCACTACAA CGGGGGCAGC
    GGCACAGGAC CCTATATTGC ACCACATCGC GGCATGCCAC CGCCAATAGG AAACCTGCCG
    CCTCACACGC CCGAACGTCA TGCTCTTGAC TTGGCCGGAA GTCGGGAGCA GCGCGGTTCG
    GCCTTCGAAC TGTATCGTAA ACCGCAAATC GGAGCTACCG CCGGGCACCA TCACAACATG
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097711.1
    CDS326..4231
    Translation

    Target ORF information:

    RefSeq Version NM_001104241.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104241.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGACGCTATC ATTTACATGC GCCCCATTTG
    AAGCACTTGC ATATGATGCA CTCGCTCTCA CCTTCGTCCT CGTTGTCGCT CTCACCTTCG
    CCCTCGTCCT TGCCCCTTCA CATGTCCTGC GGCCTTCAGC TGACACAGCA GCAGCTGCTA
    CGCCTCTCCA CCGGCGGCTT CGATCGCCGA CCCATTGCTG CACCACGTCT GCATGCCCTG
    ATGGCACCAC TGCCACCGCC AGCTCTGATG TCCAGTGCCT CGGCCTACGA TACACTCGAC
    TCTGGTTCGC CCCGTTCTCC GAATGGCTTC TATTATCCAC CAACGCCTTC GCATCATCAT
    TACGGCCAGC AGGCGACCCC GGGTTCACCC AGCAATGGCC ACATGCAGCC ACCACCACCG
    CAGCAGCAGC AGCCCACCTA CGGCGGAAGT AATGGCAACT ATGGCCAGGC ACCACCACCA
    CAATCCTATC CGCAGGCCAA TGGCTACAAT GGGAATGGGC ATCACTACAA CGGGGGCAGC
    GGCACAGGAC CCTATATTGC ACCACATCGC GGCATGCCAC CGCCAATAGG AAACCTGCCG
    CCTCACACGC CCGAACGTCA TGCTCTTGAC TTGGCCGGAA GTCGGGAGCA GCGCGGTTCG
    GCCTTCGAAC TGTATCGTAA ACCGCAAATC GGAGCTACCG CCGGGCACCA TCACAACATG
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04286
    Clone ID Related Accession (Same CDS sequence) NM_001104241.2 , NM_001260072.2
    Accession Version NM_001260072.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260072.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGACGCTATC ATTTACATGC GCCCCATTTG
    AAGCACTTGC ATATGATGCA CTCGCTCTCA CCTTCGTCCT CGTTGTCGCT CTCACCTTCG
    CCCTCGTCCT TGCCCCTTCA CATGTCCTGC GGCCTTCAGC TGACACAGCA GCAGCTGCTA
    CGCCTCTCCA CCGGCGGCTT CGATCGCCGA CCCATTGCTG CACCACGTCT GCATGCCCTG
    ATGGCACCAC TGCCACCGCC AGCTCTGATG TCCAGTGCCT CGGCCTACGA TACACTCGAC
    TCTGGTTCGC CCCGTTCTCC GAATGGCTTC TATTATCCAC CAACGCCTTC GCATCATCAT
    TACGGCCAGC AGGCGACCCC GGGTTCACCC AGCAATGGCC ACATGCAGCC ACCACCACCG
    CAGCAGCAGC AGCCCACCTA CGGCGGAAGT AATGGCAACT ATGGCCAGGC ACCACCACCA
    CAATCCTATC CGCAGGCCAA TGGCTACAAT GGGAATGGGC ATCACTACAA CGGGGGCAGC
    GGCACAGGAC CCTATATTGC ACCACATCGC GGCATGCCAC CGCCAATAGG AAACCTGCCG
    CCTCACACGC CCGAACGTCA TGCTCTTGAC TTGGCCGGAA GTCGGGAGCA GCGCGGTTCG
    GCCTTCGAAC TGTATCGTAA ACCGCAAATC GGAGCTACCG CCGGGCACCA TCACAACATG
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247001.1
    CDS1336..5241
    Translation

    Target ORF information:

    RefSeq Version NM_001260072.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260072.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGACGCTATC ATTTACATGC GCCCCATTTG
    AAGCACTTGC ATATGATGCA CTCGCTCTCA CCTTCGTCCT CGTTGTCGCT CTCACCTTCG
    CCCTCGTCCT TGCCCCTTCA CATGTCCTGC GGCCTTCAGC TGACACAGCA GCAGCTGCTA
    CGCCTCTCCA CCGGCGGCTT CGATCGCCGA CCCATTGCTG CACCACGTCT GCATGCCCTG
    ATGGCACCAC TGCCACCGCC AGCTCTGATG TCCAGTGCCT CGGCCTACGA TACACTCGAC
    TCTGGTTCGC CCCGTTCTCC GAATGGCTTC TATTATCCAC CAACGCCTTC GCATCATCAT
    TACGGCCAGC AGGCGACCCC GGGTTCACCC AGCAATGGCC ACATGCAGCC ACCACCACCG
    CAGCAGCAGC AGCCCACCTA CGGCGGAAGT AATGGCAACT ATGGCCAGGC ACCACCACCA
    CAATCCTATC CGCAGGCCAA TGGCTACAAT GGGAATGGGC ATCACTACAA CGGGGGCAGC
    GGCACAGGAC CCTATATTGC ACCACATCGC GGCATGCCAC CGCCAATAGG AAACCTGCCG
    CCTCACACGC CCGAACGTCA TGCTCTTGAC TTGGCCGGAA GTCGGGAGCA GCGCGGTTCG
    GCCTTCGAAC TGTATCGTAA ACCGCAAATC GGAGCTACCG CCGGGCACCA TCACAACATG
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04287
    Clone ID Related Accession (Same CDS sequence) NM_176426.4
    Accession Version NM_176426.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176426.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA
    TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG
    AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC
    TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT
    GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG CTGTCCCCGC GAATCCCAAT
    CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAATGT GCCACCACAG
    CAGCAGCAGC CCGGCGTTGG AGGAGATACG GCTGCAGTGA GACCGCAATC TCTGTACGGG
    ATTAATGCTC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG CGATATACCA
    CTGAACACCT CGGGCTCATC GTCGCGAAAT GGCACCCTGG GTCGAAGTGC CCAGGAGCGT
    CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AGTACATAAC CCGAAACGCT
    CTGGTCGGGT CTGGAGCTGC AGAAACCCTG GATCGCCAGC AGCAGCAGCA GCAGCAGACT
    CATGCATCCT TGGAGCGCCA AGCGCGTTTG AATGCGCAGC TAAAGGCCAA CGGAGTAGGC
    GCCGGAGGAG GTGGTGCATC CACCTACGAC AGTGTGTCCA GCTACGACTC GTACAACAAT
    ACACAAATGG CCATGCAAAA CCTGGGCCGA CTAGGTCCCA ATGCACCCGA CGATCTCAAA
    TCAGTGCCAA ATGCAAATGG TCGCCCCTTG CCTCCGACGG GTCAGTCGCA CGAGTACGGT
    CGCACTCCGC ACGATCATCG CAGCTTCGGA GGACCCAACG ATCTGAACCG GCAGAGCAGT
    CCGGGCAGGC CGCACTACCA TGACATGAAT GCGTCCCGTA ATATCGATCC ACGCAATGGC
    ACACCACAGC GTCCTTCGAA TCTGGGCCTG GAAAGCAGCC CACGCAAGCC TTTGGTGGAG
    ACAAAAACGG ATTACGGCAA ATACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788603.1
    CDS326..3775
    Translation

    Target ORF information:

    RefSeq Version NM_176426.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176426.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA
    TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG
    AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC
    TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT
    GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG CTGTCCCCGC GAATCCCAAT
    CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAATGT GCCACCACAG
    CAGCAGCAGC CCGGCGTTGG AGGAGATACG GCTGCAGTGA GACCGCAATC TCTGTACGGG
    ATTAATGCTC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG CGATATACCA
    CTGAACACCT CGGGCTCATC GTCGCGAAAT GGCACCCTGG GTCGAAGTGC CCAGGAGCGT
    CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AGTACATAAC CCGAAACGCT
    CTGGTCGGGT CTGGAGCTGC AGAAACCCTG GATCGCCAGC AGCAGCAGCA GCAGCAGACT
    CATGCATCCT TGGAGCGCCA AGCGCGTTTG AATGCGCAGC TAAAGGCCAA CGGAGTAGGC
    GCCGGAGGAG GTGGTGCATC CACCTACGAC AGTGTGTCCA GCTACGACTC GTACAACAAT
    ACACAAATGG CCATGCAAAA CCTGGGCCGA CTAGGTCCCA ATGCACCCGA CGATCTCAAA
    TCAGTGCCAA ATGCAAATGG TCGCCCCTTG CCTCCGACGG GTCAGTCGCA CGAGTACGGT
    CGCACTCCGC ACGATCATCG CAGCTTCGGA GGACCCAACG ATCTGAACCG GCAGAGCAGT
    CCGGGCAGGC CGCACTACCA TGACATGAAT GCGTCCCGTA ATATCGATCC ACGCAATGGC
    ACACCACAGC GTCCTTCGAA TCTGGGCCTG GAAAGCAGCC CACGCAAGCC TTTGGTGGAG
    ACAAAAACGG ATTACGGCAA ATACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04282
    Clone ID Related Accession (Same CDS sequence) NM_057349.7
    Accession Version NM_057349.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057349.6. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA
    TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG
    AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC
    TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT
    GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG CTGTCCCCGC GAATCCCAAT
    CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAATGT GCCACCACAG
    CAGCAGCAGC CCGGCGTTGG AGGAGATACG GCTGCAGTGA GACCGCAATC TCTGTACGGG
    ATTAATGCTC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG CGATATACCA
    CTGAACACCT CGGGCTCATC GTCGCGAAAT GGCACCCTGG GTCGAAGTGC CCAGGAGCGT
    CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AGTACATAAC CCGAAACGCT
    CTGGTCGGGT CTGGAGCTGC AGAAACCCTG GATCGCCAGC AGCAGCAGCA GCAGCAGACT
    CATGCATCCT TGGAGCGCCA AGCGCGTTTG AATGCGCAGC TAAAGGCCAA CGGAGTAGGC
    GCCGGAGGAG GTGGTGCATC CACCTACGAC AGTGTGTCCA GCTACGACTC GTACAACAAT
    ACACAAATGG CCATGCAAAA CCTGGGCCGA CTAGGTCCCA ATGCACCCGA CGATCTCAAA
    TCAGTGCCAA ATGCAAATGG TCGCCCCTTG CCTCCGACGG GTCAGTCGCA CGAGTACGGT
    CGCACTCCGC ACGATCATCG CAGCTTCGGA GGACCCAACG ATCTGAACCG GCAGAGCAGT
    CCGGGCAGGC CGCACTACCA TGACATGAAT GCGTCCCGTA ATATCGATCC ACGCAATGGC
    ACACCACAGC GTCCTTCGAA TCTGGGCCTG GAAAGCAGCC CACGCAAGCC TTTGGTGGAG
    ACAAAAACGG ATTACGGCAA ATACAGTCGG AATAACTCCG TAACGCAAGC GGATTATACA
    AAGCTACCGA AGACTGCGCC ACATGGAGTA GTTCCTCCGC CGAATGTGAG CAATGGCCAG
    GGCCAGATGA ATGGCAGCGG AACGCCGAGC AGCAATGGCA GTGGACCCTT CAAGCCGGTG
    CCACCACCCA AACCCAAAAA CTATAGGCCA CCGGTGCAAA GTGGTGGTAG CAGTGGCAGC
    GGAGGCACAA CTCCTTGGGA GAATGGGGAC TCTGGTTCGC CCCGTTCTCC GAATGGCTTC
    TATTATCCAC CAACGCCTTC GCATCATCAT TACGGCCAGC AGGCGACCCC GGGTTCACCC
    AGCAATGGCC ACATGCAGCC ACCACCACCG CAGCAGCAGC AGCCCACCTA CGGCGGAAGT
    AATGGCAACT ATGGCCAGGC ACCACCACCA CAATCCTATC CGCAGGCCAA TGGCTACAAT
    GGGAATGGGC ATCACTACAA CGGGGGCAGC GGCACAGGAC CCTATATTGC ACCACATCGC
    GGCATGCCAC CGCCAATAGG AAACCTGCCG CCTCACACGC CCGAACGTCA TGCTCTTGAC
    TTGGCCGGAA GTCGGGAGCA GCGCGGTTCG GCCTTCGAAC TGTATCGTAA ACCGCAAATC
    GGAGCTACCG CCGGGCACCA TCACAACATG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_476697.3
    CDS326..4441
    Translation

    Target ORF information:

    RefSeq Version NM_057349.7
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057349.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA
    TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG
    AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC
    TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT
    GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG CTGTCCCCGC GAATCCCAAT
    CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAATGT GCCACCACAG
    CAGCAGCAGC CCGGCGTTGG AGGAGATACG GCTGCAGTGA GACCGCAATC TCTGTACGGG
    ATTAATGCTC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG CGATATACCA
    CTGAACACCT CGGGCTCATC GTCGCGAAAT GGCACCCTGG GTCGAAGTGC CCAGGAGCGT
    CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AGTACATAAC CCGAAACGCT
    CTGGTCGGGT CTGGAGCTGC AGAAACCCTG GATCGCCAGC AGCAGCAGCA GCAGCAGACT
    CATGCATCCT TGGAGCGCCA AGCGCGTTTG AATGCGCAGC TAAAGGCCAA CGGAGTAGGC
    GCCGGAGGAG GTGGTGCATC CACCTACGAC AGTGTGTCCA GCTACGACTC GTACAACAAT
    ACACAAATGG CCATGCAAAA CCTGGGCCGA CTAGGTCCCA ATGCACCCGA CGATCTCAAA
    TCAGTGCCAA ATGCAAATGG TCGCCCCTTG CCTCCGACGG GTCAGTCGCA CGAGTACGGT
    CGCACTCCGC ACGATCATCG CAGCTTCGGA GGACCCAACG ATCTGAACCG GCAGAGCAGT
    CCGGGCAGGC CGCACTACCA TGACATGAAT GCGTCCCGTA ATATCGATCC ACGCAATGGC
    ACACCACAGC GTCCTTCGAA TCTGGGCCTG GAAAGCAGCC CACGCAAGCC TTTGGTGGAG
    ACAAAAACGG ATTACGGCAA ATACAGTCGG AATAACTCCG TAACGCAAGC GGATTATACA
    AAGCTACCGA AGACTGCGCC ACATGGAGTA GTTCCTCCGC CGAATGTGAG CAATGGCCAG
    GGCCAGATGA ATGGCAGCGG AACGCCGAGC AGCAATGGCA GTGGACCCTT CAAGCCGGTG
    CCACCACCCA AACCCAAAAA CTATAGGCCA CCGGTGCAAA GTGGTGGTAG CAGTGGCAGC
    GGAGGCACAA CTCCTTGGGA GAATGGGGAC TCTGGTTCGC CCCGTTCTCC GAATGGCTTC
    TATTATCCAC CAACGCCTTC GCATCATCAT TACGGCCAGC AGGCGACCCC GGGTTCACCC
    AGCAATGGCC ACATGCAGCC ACCACCACCG CAGCAGCAGC AGCCCACCTA CGGCGGAAGT
    AATGGCAACT ATGGCCAGGC ACCACCACCA CAATCCTATC CGCAGGCCAA TGGCTACAAT
    GGGAATGGGC ATCACTACAA CGGGGGCAGC GGCACAGGAC CCTATATTGC ACCACATCGC
    GGCATGCCAC CGCCAATAGG AAACCTGCCG CCTCACACGC CCGAACGTCA TGCTCTTGAC
    TTGGCCGGAA GTCGGGAGCA GCGCGGTTCG GCCTTCGAAC TGTATCGTAA ACCGCAAATC
    GGAGCTACCG CCGGGCACCA TCACAACATG AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07909
    Clone ID Related Accession (Same CDS sequence) NM_001144556.2
    Accession Version NM_001144556.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144556.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATATAG TCAGCGGATC GGCAACGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
    CGCCATGAAT CTTTTCAGCA ACAGGGTGAT CGAACCACAT GGGAGTACCA CACGGTGGCG
    GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG
    CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC
    GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC TCTGGAGAAC
    GTGGAATATG CTACCGCGGT GCAGGTGTTA CGCGATAGTG GCAACACGGT GCAATTGGTG
    GTCAAGCGTC GCGTGCCCCT CAATCCCATT AATGCTGCAG GTGCAGTGCA GCATCAGCAT
    TCACACAGTC TCAGTTCGGT TGGTCTAATG GCCAATGGAA GTGGAGGAGT GGCGCCAACC
    CCCATTACGA GTCTGAGTCA ACCCAACTCG CTGAACTCTT CCCTAGTGCA GAATGCCAGC
    AGTGGTCAGC CCATCAAGGT GACCCTCACC AAAGGCGGAA AGAAAGATGA CTATGGTGTG
    GTGCTCGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCTA AAGCTCGCGA GCAACTAAAT
    GCCAATGGTT ATAGCTTGCA AGAGGGTGAC ATCATCACCC GCATCCACAA TACTAACTGC
    GGGGATACGA TGAGTCTTAA GGAGGCGAAA AAGATCATTG ATGGCTGCAA GGAGCGATTG
    AACCTAGTGG TTCTAAGGGA CATTACCAAC CAAACAGCTG TTAGCCAATT GAATCTGAAC
    AATTCAGCCA GCCACCAGGC GTCGGGAAAT ATATACGCCA CGCATCAGCC TCAGGTATCC
    GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC
    TCCTCGCAGA ATCTGTACGT GCAACCGCCA ACTCGCACCT CCAATGGTCC CAATATCAAT
    GGCAATGGCC TGAACGACGA AAAGAGTAAT CTTACGCCGC GAGGTCGCTC CCGGGGCCCC
    ATAATGGATG GGGTATCCCT GCAGCAACTG GATAGACCCG TAACACCGAC TCGGGGCAGG
    AGTGCAGCCA TCGACGAGCC GCCGCGTCCA CCACCACCAA GGGGATCGAG CGGAGGAGCA
    GCTCAAGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGTCA GAGTGCCGAA
    CCGAGATTCA TTTCCTTCCA GAAGGAAGGA AGCGTAGGCA TCCGGCTAAC CGGCGGCAAT
    GAAGCTGGTA TTTTTGTGAC TGCCGTACAA CCAGGATCTC CAGCCTCGCT GCAGGGTCTA
    ATGCCTGGCG ATAAGATCCT CAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG
    GAGGCTGTTC TCTTCCTGTT GAGTCTCCAG GATCGCATCG ATCTGATTGT GCAGTACTGC
    AAGGAGGAAT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA
    CATTTCCACT GCGATAATCC TTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTTTTC
    CGGGTGATCG ATACCCTGCA CAATGGTGTG GTCGGATCCT GGCAGGTGCT GAAGATCGGT
    CGGGGTCACC AGGAGATGCA GCGCGGCGTA ATACCGAACA AGTCGCGGGC TGAAGAGCTG
    GCCACTGCTC AGTTCAATGC CACCAAGAAG GAGATGAATG CAAATGAATC GAGAGGCAAC
    TTCTTCCGGC GACGACGTTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG
    GACGATGTGG TCTTCTCCGA TAGCATTTCC AAATTCCCCG CCTACGAGCG TGTCGTTCTG
    CGCCATCCAG GCTTTGTCCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TTTGGCCCGC
    GAGCGATTGG CCAAAGATTT CCCCGATAAG TTTTCGACTC CACTGCAGGA TGACGACAAG
    TCAGCCGCAA CTAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAATATACG CGATGTGATG
    GATCGTGGAA AGCACGCCCT GCTGGACATT ACGCCCAATG CAGTGGATCG TCTCAATTAC
    GCGCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG
    CTGCGTCACG GACTTCCCAA GGCGGCACAC AAGAGCTCCA AGAAGCTGCT GGAGCAGTGC
    CAGAAACTGG AGCGCGTCTG GTCACACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG
    GAGTCCTGGT ACCGAAAGTT GCGCGATTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG
    ATGTCCGAGT CTAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA
    TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC
    GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT
    GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT
    CCATATACGG TACCCTACGA CCATGCTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG
    GACTCCAGCA AGTACAGCAT CTACGGCACC AATGTGCCAC CACAGCAGCA GCAGCCCGGC
    GTTGGAGGAG ATACGGCTGC AGTGAGACCG CAATCTCTGT ACGGGATTAA TGCTCCCGAT
    CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGCGATA TACCACTGAA CACCTCGGGC
    TCATCGTCGC GAAATGGCAC CCTGGGTCGA AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT
    GTGGTGCAGG ACGATGTCCA GGCGGAGTAC ATAACCCGAA ACGCTCTGGT CGGGTCTGGA
    GCTGCAGAAA CCCTGGATCG CCAGCAGCAG CAGCAGCAGC AGACTCATGC ATCCTTGGAG
    CGCCAAGCGC GTTTGAATGC GCAGCTAAAG GCCAACGGAG TAGGCGCCGG AGGAGGTGGT
    GCATCCACCT ACGACAGTGT GTCCAGCTAC GACTCGTACA ACAATACACA AATGGCCATG
    CAAAACCTGG GCCGACTAGG TCCCAATGCA CCCGACGATC TCAAATCAGT GCCAAATGCA
    AATGGTCGCC CCTTGCCTCC GACGGGTCAG TCGCACGAGT ACGGTCGCAC TCCGCACGAT
    CATCGCAGCT TCGGAGGACC CAACGATCTG AACCGGCAGA GCAGTCCGGG CAGGCCGCAC
    TACCATGACA TGAATGCGTC CCGTAATATC GATCCACGCA ATGGCACACC ACAGCGTCCT
    TCGAATCTGG GCCTGGAAAG CAGCCCACGC AAGCCTTTGG TGGAGACAAA AACGGATTAC
    GGCAAATACA GTCGGAATAA CTCCGTAACG CAAGCGGATT ATACAAAGCT ACCGAAGACT
    GCGCCACATG GAGTAGTTCC TCCGCCGAAT GTGAGCAATG GCCAGGGCCA GATGAATGGC
    AGCGGAACGC CGAGCAGCAA TGGCAGTGGA CCCTTCAAGC CGGTGCCACC ACCCAAACCC
    AAAAACTATA GGCCACCGGT GCAAAGTGGT GGTAGCAGTG GCAGCGGAGG CACAACTCCT
    TGGGAGAATG GGGACTCTGG TTCGCCCCGT TCTCCGAATG GCTTCTATTA TCCACCAACG
    CCTTCGCATC ATCATTACGG CCAGCAGGCG ACCCCGGGTT CACCCAGCAA TGGCCACATG
    CAGCCACCAC CACCGCAGCA GCAGCAGCCC ACCTACGGCG GAAGTAATGG CAACTATGGC
    CAGGCACCAC CACCACAATC CTATCCGCAG GCCAATGGCT ACAATGGGAA TGGGCATCAC
    TACAACGGGG GCAGCGGCAC AGGACCCTAT ATTGCACCAC ATCGCGGCAT GCCACCGCCA
    ATAGGAAACC TGCCGCCTCA CACGCCCGAA CGTCATGCTC TTGACTTGGC CGGAAGTCGG
    GAGCAGCGCG GTTCGGCCTT CGAACTGTAT CGTAAACCGC AAATCGGAGC TACCGCCGGG
    CACCATCACA ACATGAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138028.1
    CDS528..4688
    Translation

    Target ORF information:

    RefSeq Version NM_001144556.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144556.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGATATAG TCAGCGGATC GGCAACGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
    CGCCATGAAT CTTTTCAGCA ACAGGGTGAT CGAACCACAT GGGAGTACCA CACGGTGGCG
    GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG
    CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC
    GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC TCTGGAGAAC
    GTGGAATATG CTACCGCGGT GCAGGTGTTA CGCGATAGTG GCAACACGGT GCAATTGGTG
    GTCAAGCGTC GCGTGCCCCT CAATCCCATT AATGCTGCAG GTGCAGTGCA GCATCAGCAT
    TCACACAGTC TCAGTTCGGT TGGTCTAATG GCCAATGGAA GTGGAGGAGT GGCGCCAACC
    CCCATTACGA GTCTGAGTCA ACCCAACTCG CTGAACTCTT CCCTAGTGCA GAATGCCAGC
    AGTGGTCAGC CCATCAAGGT GACCCTCACC AAAGGCGGAA AGAAAGATGA CTATGGTGTG
    GTGCTCGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCTA AAGCTCGCGA GCAACTAAAT
    GCCAATGGTT ATAGCTTGCA AGAGGGTGAC ATCATCACCC GCATCCACAA TACTAACTGC
    GGGGATACGA TGAGTCTTAA GGAGGCGAAA AAGATCATTG ATGGCTGCAA GGAGCGATTG
    AACCTAGTGG TTCTAAGGGA CATTACCAAC CAAACAGCTG TTAGCCAATT GAATCTGAAC
    AATTCAGCCA GCCACCAGGC GTCGGGAAAT ATATACGCCA CGCATCAGCC TCAGGTATCC
    GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC
    TCCTCGCAGA ATCTGTACGT GCAACCGCCA ACTCGCACCT CCAATGGTCC CAATATCAAT
    GGCAATGGCC TGAACGACGA AAAGAGTAAT CTTACGCCGC GAGGTCGCTC CCGGGGCCCC
    ATAATGGATG GGGTATCCCT GCAGCAACTG GATAGACCCG TAACACCGAC TCGGGGCAGG
    AGTGCAGCCA TCGACGAGCC GCCGCGTCCA CCACCACCAA GGGGATCGAG CGGAGGAGCA
    GCTCAAGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGTCA GAGTGCCGAA
    CCGAGATTCA TTTCCTTCCA GAAGGAAGGA AGCGTAGGCA TCCGGCTAAC CGGCGGCAAT
    GAAGCTGGTA TTTTTGTGAC TGCCGTACAA CCAGGATCTC CAGCCTCGCT GCAGGGTCTA
    ATGCCTGGCG ATAAGATCCT CAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG
    GAGGCTGTTC TCTTCCTGTT GAGTCTCCAG GATCGCATCG ATCTGATTGT GCAGTACTGC
    AAGGAGGAAT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA
    CATTTCCACT GCGATAATCC TTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTTTTC
    CGGGTGATCG ATACCCTGCA CAATGGTGTG GTCGGATCCT GGCAGGTGCT GAAGATCGGT
    CGGGGTCACC AGGAGATGCA GCGCGGCGTA ATACCGAACA AGTCGCGGGC TGAAGAGCTG
    GCCACTGCTC AGTTCAATGC CACCAAGAAG GAGATGAATG CAAATGAATC GAGAGGCAAC
    TTCTTCCGGC GACGACGTTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG
    GACGATGTGG TCTTCTCCGA TAGCATTTCC AAATTCCCCG CCTACGAGCG TGTCGTTCTG
    CGCCATCCAG GCTTTGTCCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TTTGGCCCGC
    GAGCGATTGG CCAAAGATTT CCCCGATAAG TTTTCGACTC CACTGCAGGA TGACGACAAG
    TCAGCCGCAA CTAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAATATACG CGATGTGATG
    GATCGTGGAA AGCACGCCCT GCTGGACATT ACGCCCAATG CAGTGGATCG TCTCAATTAC
    GCGCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG
    CTGCGTCACG GACTTCCCAA GGCGGCACAC AAGAGCTCCA AGAAGCTGCT GGAGCAGTGC
    CAGAAACTGG AGCGCGTCTG GTCACACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG
    GAGTCCTGGT ACCGAAAGTT GCGCGATTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG
    ATGTCCGAGT CTAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA
    TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC
    GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT
    GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT
    CCATATACGG TACCCTACGA CCATGCTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG
    GACTCCAGCA AGTACAGCAT CTACGGCACC AATGTGCCAC CACAGCAGCA GCAGCCCGGC
    GTTGGAGGAG ATACGGCTGC AGTGAGACCG CAATCTCTGT ACGGGATTAA TGCTCCCGAT
    CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGCGATA TACCACTGAA CACCTCGGGC
    TCATCGTCGC GAAATGGCAC CCTGGGTCGA AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT
    GTGGTGCAGG ACGATGTCCA GGCGGAGTAC ATAACCCGAA ACGCTCTGGT CGGGTCTGGA
    GCTGCAGAAA CCCTGGATCG CCAGCAGCAG CAGCAGCAGC AGACTCATGC ATCCTTGGAG
    CGCCAAGCGC GTTTGAATGC GCAGCTAAAG GCCAACGGAG TAGGCGCCGG AGGAGGTGGT
    GCATCCACCT ACGACAGTGT GTCCAGCTAC GACTCGTACA ACAATACACA AATGGCCATG
    CAAAACCTGG GCCGACTAGG TCCCAATGCA CCCGACGATC TCAAATCAGT GCCAAATGCA
    AATGGTCGCC CCTTGCCTCC GACGGGTCAG TCGCACGAGT ACGGTCGCAC TCCGCACGAT
    CATCGCAGCT TCGGAGGACC CAACGATCTG AACCGGCAGA GCAGTCCGGG CAGGCCGCAC
    TACCATGACA TGAATGCGTC CCGTAATATC GATCCACGCA ATGGCACACC ACAGCGTCCT
    TCGAATCTGG GCCTGGAAAG CAGCCCACGC AAGCCTTTGG TGGAGACAAA AACGGATTAC
    GGCAAATACA GTCGGAATAA CTCCGTAACG CAAGCGGATT ATACAAAGCT ACCGAAGACT
    GCGCCACATG GAGTAGTTCC TCCGCCGAAT GTGAGCAATG GCCAGGGCCA GATGAATGGC
    AGCGGAACGC CGAGCAGCAA TGGCAGTGGA CCCTTCAAGC CGGTGCCACC ACCCAAACCC
    AAAAACTATA GGCCACCGGT GCAAAGTGGT GGTAGCAGTG GCAGCGGAGG CACAACTCCT
    TGGGAGAATG GGGACTCTGG TTCGCCCCGT TCTCCGAATG GCTTCTATTA TCCACCAACG
    CCTTCGCATC ATCATTACGG CCAGCAGGCG ACCCCGGGTT CACCCAGCAA TGGCCACATG
    CAGCCACCAC CACCGCAGCA GCAGCAGCCC ACCTACGGCG GAAGTAATGG CAACTATGGC
    CAGGCACCAC CACCACAATC CTATCCGCAG GCCAATGGCT ACAATGGGAA TGGGCATCAC
    TACAACGGGG GCAGCGGCAC AGGACCCTAT ATTGCACCAC ATCGCGGCAT GCCACCGCCA
    ATAGGAAACC TGCCGCCTCA CACGCCCGAA CGTCATGCTC TTGACTTGGC CGGAAGTCGG
    GAGCAGCGCG GTTCGGCCTT CGAACTGTAT CGTAAACCGC AAATCGGAGC TACCGCCGGG
    CACCATCACA ACATGAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04283
    Clone ID Related Accession (Same CDS sequence) NM_170646.6
    Accession Version NM_170646.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170646.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCACTGCTCT CCCGTCACCA GGAGCGGATT 
    ATGCTGGACC TGGGGAGCAC CGATCTGTTA GCGGTTCTGG TTAAGAACTC GGTGCTTAGT
    CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TTACGCCCAC GCCTTCTGCT
    GCCGCCTCTG CCACTGTGGA GCCTCCCGGT AGTGCACCCT CGCTCACCAA GCGGAAACTG
    AGTGCCGTGG CCAGTAGTGG GAGTACGGCC AGCGGGGGGA GTGCCGGCAG CGGAGGCAGT
    GGCTCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAATT GCGACAGTCG CTCACTAACG
    CCCTCTGGCG GGGGACCATG TTCTGGGCTA AACGACGCCG AAATTCTGCG AGTCCAGTGC
    TCCAATCTGA TCGATATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGCTAC
    GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG CCTCAAGACC
    GATGCCGCCA ATTTGGAGGA CAGTCGCGAT GGTGAAAAAA TGCAAAAGGA AATCGAAACG
    ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGCGCTGT TAGTTATGGC
    GGTGACGCCA CCTCCTCCCC CCATTCGGCC ACCATACCCC GCGCCGCCCC CCGTCGGGTG
    GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG TTCCAAAGCG
    GATTCTGTAT CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA
    ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCT
    AATACTGGCA CCATATCCTC TACGAATGGC TACGGATCGC TGTTATGTCA CGCCAGTTCG
    ACGACCAGTC CGCTGGCGGT CCGGAAGAGG GACAAACTGC TTCATCGCTT CAGCGATGCC
    GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG
    ACGGAGGCCA TTGAGATGCT GGCCGATCCC GTCATCGAAG ATGAATTCTT TGGTGATCGA
    ACCACATGGG AGTACCACAC GGTGGCGGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC
    GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT
    AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC
    TCGGTGAACG GCGTCTCTCT GGAGAACGTG GAATATGCTA CCGCGGTGCA GGTGTTACGC
    GATAGTGGCA ACACGGTGCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATTAAT
    GCTGCAGGTG CAGTGCAGCA TCAGCATTCA CACAGTCTCA GTTCGGTTGG TCTAATGGCC
    AATGGAAGTG GAGGAGTGGC GCCAACCCCC ATTACGAGTC TGAGTCAACC CAACTCGCTG
    AACTCTTCCC TAGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACCAAA
    GGCGGAAAGA AAGATGACTA TGGTGTGGTG CTCGGCTGCC GGCTGTTTGT TAAGGAGATC
    TCCTCTAAAG CTCGCGAGCA ACTAAATGCC AATGGTTATA GCTTGCAAGA GGGTGACATC
    ATCACCCGCA TCCACAATAC TAACTGCGGG GATACGATGA GTCTTAAGGA GGCGAAAAAG
    ATCATTGATG GCTGCAAGGA GCGATTGAAC CTAGTGGTTC TAAGGGACAT TACCAACCAA
    ACAGCTGTTA GCCAATTGAA TCTGAACAAT TCAGCCAGCC ACCAGGCGTC GGGAAATATA
    TACGCCACGC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT
    CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCGCCAACT
    CGCACCTCCA ATGGTCCCAA TATCAATGGC AATGGCCTGA ACGACGAAAA GAGTAATCTT
    ACGCCGCGAG GTCGCTCCCG GGGCCCCATA ATGGATGGGG TATCCCTGCA GCAACTGGAT
    AGACCCGTAA CACCGACTCG GGGCAGGAGT GCAGCCATCG ACGAGCCGCC GCGTCCACCA
    CCACCAAGGG GATCGAGCGG AGGAGCAGCT CAAGAGGATT TCTACAGCTC TCGCAGGCAA
    TTGTACGAGG AGCGTCAGAG TGCCGAACCG AGATTCATTT CCTTCCAGAA GGAAGGAAGC
    GTAGGCATCC GGCTAACCGG CGGCAATGAA GCTGGTATTT TTGTGACTGC CGTACAACCA
    GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCTGGCGATA AGATCCTCAA GGTCAACGAC
    ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TCCTGTTGAG TCTCCAGGAT
    CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAATACG ACGAGGTGGT GACCAACCAA
    CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCTTC CAAAGGCGAG
    ATGGCCTTCA AGGCGGGCGA TGTTTTCCGG GTGATCGATA CCCTGCACAA TGGTGTGGTC
    GGATCCTGGC AGGTGCTGAA GATCGGTCGG GGTCACCAGG AGATGCAGCG CGGCGTAATA
    CCGAACAAGT CGCGGGCTGA AGAGCTGGCC ACTGCTCAGT TCAATGCCAC CAAGAAGGAG
    ATGAATGCAA ATGAATCGAG AGGCAACTTC TTCCGGCGAC GACGTTCGAC GCACCGACGC
    TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GATGTGGTCT TCTCCGATAG CATTTCCAAA
    TTCCCCGCCT ACGAGCGTGT CGTTCTGCGC CATCCAGGCT TTGTCCGACC CGTAGTGCTC
    TTCGGACCCG TATCCGATTT GGCCCGCGAG CGATTGGCCA AAGATTTCCC CGATAAGTTT
    TCGACTCCAC TGCAGGATGA CGACAAGTCA GCCGCAACTA GTGGAAAGTG CCGCATCGTA
    AGGCTCTCGA ATATACGCGA TGTGATGGAT CGTGGAAAGC ACGCCCTGCT GGACATTACG
    CCCAATGCAG TGGATCGTCT CAATTACGCG CAGTTCTATC CGGTGGTGAT CTTCCTGAAG
    ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGTCACGGAC TTCCCAAGGC GGCACACAAG
    AGCTCCAAGA AGCTGCTGGA GCAGTGCCAG AAACTGGAGC GCGTCTGGTC ACACATCTTT
    AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGATTCGATC
    GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCTA AGCCAGTAGA ATCCCTCTCC
    GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC
    GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG
    GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA
    GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGCTGTCCCC
    GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAT
    GTGCCACCAC AGCAGCAGCA GCCCGGCGTT GGAGGAGATA CGGCTGCAGT GAGACCGCAA
    TCTCTGTACG GGATTAATGC TCCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG
    GGCGATATAC CACTGAACAC CTCGGGCTCA TCGTCGCGAA ATGGCACCCT GGGTCGAAGT
    GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAGTACATA
    ACCCGAAACG CTCTGGTCGG GTCTGGAGCT GCAGAAACCC TGGATCGCCA GCAGCAGCAG
    CAGCAGCAGA CTCATGCATC CTTGGAGCGC CAAGCGCGTT TGAATGCGCA GCTAAAGGCC
    AACGGAGTAG GCGCCGGAGG AGGTGGTGCA TCCACCTACG ACAGTGTGTC CAGCTACGAC
    TCGTACAACA ATACACAAAT GGCCATGCAA AACCTGGGCC GACTAGGTCC CAATGCACCC
    GACGATCTCA AATCAGTGCC AAATGCAAAT GGTCGCCCCT TGCCTCCGAC GGGTCAGTCG
    CACGAGTACG GTCGCACTCC GCACGATCAT CGCAGCTTCG GAGGACCCAA CGATCTGAAC
    CGGCAGAGCA GTCCGGGCAG GCCGCACTAC CATGACATGA ATGCGTCCCG TAATATCGAT
    CCACGCAATG GCACACCACA GCGTCCTTCG AATCTGGGCC TGGAAAGCAG CCCACGCAAG
    CCTTTGGTGG AGACAAAAAC GGATTACGGC AAATACAGTC GGAATAACTC CGTAACGCAA
    GCGGATTATA CAAAGCTACC GAAGACTGCG CCACATGGAG TAGTTCCTCC GCCGAATGTG
    AGCAATGGCC AGGGCCAGAT GAATGGCAGC GGAACGCCGA GCAGCAATGG CAGTGGACCC
    TTCAAGCCGG TGCCACCACC CAAACCCAAA AACTATAGGC CACCGGTGCA AAGTGGTGGT
    AGCAGTGGCA GCGGAGGCAC AACTCCTTGG GAGAATGGGG ACTCTGGTTC GCCCCGTTCT
    CCGAATGGCT TCTATTATCC ACCAACGCCT TCGCATCATC ATTACGGCCA GCAGGCGACC
    CCGGGTTCAC CCAGCAATGG CCACATGCAG CCACCACCAC CGCAGCAGCA GCAGCCCACC
    TACGGCGGAA GTAATGGCAA CTATGGCCAG GCACCACCAC CACAATCCTA TCCGCAGGCC
    AATGGCTACA ATGGGAATGG GCATCACTAC AACGGGGGCA GCGGCACAGG ACCCTATATT
    GCACCACATC GCGGCATGCC ACCGCCAATA GGAAACCTGC CGCCTCACAC GCCCGAACGT
    CATGCTCTTG ACTTGGCCGG AAGTCGGGAG CAGCGCGGTT CGGCCTTCGA ACTGTATCGT
    AAACCGCAAA TCGGAGCTAC CGCCGGGCAC CATCACAACA TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731293.3
    CDS641..5848
    Translation

    Target ORF information:

    RefSeq Version NM_170646.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170646.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCACTGCTCT CCCGTCACCA GGAGCGGATT 
    ATGCTGGACC TGGGGAGCAC CGATCTGTTA GCGGTTCTGG TTAAGAACTC GGTGCTTAGT
    CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TTACGCCCAC GCCTTCTGCT
    GCCGCCTCTG CCACTGTGGA GCCTCCCGGT AGTGCACCCT CGCTCACCAA GCGGAAACTG
    AGTGCCGTGG CCAGTAGTGG GAGTACGGCC AGCGGGGGGA GTGCCGGCAG CGGAGGCAGT
    GGCTCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAATT GCGACAGTCG CTCACTAACG
    CCCTCTGGCG GGGGACCATG TTCTGGGCTA AACGACGCCG AAATTCTGCG AGTCCAGTGC
    TCCAATCTGA TCGATATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGCTAC
    GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG CCTCAAGACC
    GATGCCGCCA ATTTGGAGGA CAGTCGCGAT GGTGAAAAAA TGCAAAAGGA AATCGAAACG
    ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGCGCTGT TAGTTATGGC
    GGTGACGCCA CCTCCTCCCC CCATTCGGCC ACCATACCCC GCGCCGCCCC CCGTCGGGTG
    GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG TTCCAAAGCG
    GATTCTGTAT CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA
    ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCT
    AATACTGGCA CCATATCCTC TACGAATGGC TACGGATCGC TGTTATGTCA CGCCAGTTCG
    ACGACCAGTC CGCTGGCGGT CCGGAAGAGG GACAAACTGC TTCATCGCTT CAGCGATGCC
    GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG
    ACGGAGGCCA TTGAGATGCT GGCCGATCCC GTCATCGAAG ATGAATTCTT TGGTGATCGA
    ACCACATGGG AGTACCACAC GGTGGCGGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC
    GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT
    AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC
    TCGGTGAACG GCGTCTCTCT GGAGAACGTG GAATATGCTA CCGCGGTGCA GGTGTTACGC
    GATAGTGGCA ACACGGTGCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATTAAT
    GCTGCAGGTG CAGTGCAGCA TCAGCATTCA CACAGTCTCA GTTCGGTTGG TCTAATGGCC
    AATGGAAGTG GAGGAGTGGC GCCAACCCCC ATTACGAGTC TGAGTCAACC CAACTCGCTG
    AACTCTTCCC TAGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACCAAA
    GGCGGAAAGA AAGATGACTA TGGTGTGGTG CTCGGCTGCC GGCTGTTTGT TAAGGAGATC
    TCCTCTAAAG CTCGCGAGCA ACTAAATGCC AATGGTTATA GCTTGCAAGA GGGTGACATC
    ATCACCCGCA TCCACAATAC TAACTGCGGG GATACGATGA GTCTTAAGGA GGCGAAAAAG
    ATCATTGATG GCTGCAAGGA GCGATTGAAC CTAGTGGTTC TAAGGGACAT TACCAACCAA
    ACAGCTGTTA GCCAATTGAA TCTGAACAAT TCAGCCAGCC ACCAGGCGTC GGGAAATATA
    TACGCCACGC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT
    CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCGCCAACT
    CGCACCTCCA ATGGTCCCAA TATCAATGGC AATGGCCTGA ACGACGAAAA GAGTAATCTT
    ACGCCGCGAG GTCGCTCCCG GGGCCCCATA ATGGATGGGG TATCCCTGCA GCAACTGGAT
    AGACCCGTAA CACCGACTCG GGGCAGGAGT GCAGCCATCG ACGAGCCGCC GCGTCCACCA
    CCACCAAGGG GATCGAGCGG AGGAGCAGCT CAAGAGGATT TCTACAGCTC TCGCAGGCAA
    TTGTACGAGG AGCGTCAGAG TGCCGAACCG AGATTCATTT CCTTCCAGAA GGAAGGAAGC
    GTAGGCATCC GGCTAACCGG CGGCAATGAA GCTGGTATTT TTGTGACTGC CGTACAACCA
    GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCTGGCGATA AGATCCTCAA GGTCAACGAC
    ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TCCTGTTGAG TCTCCAGGAT
    CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAATACG ACGAGGTGGT GACCAACCAA
    CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCTTC CAAAGGCGAG
    ATGGCCTTCA AGGCGGGCGA TGTTTTCCGG GTGATCGATA CCCTGCACAA TGGTGTGGTC
    GGATCCTGGC AGGTGCTGAA GATCGGTCGG GGTCACCAGG AGATGCAGCG CGGCGTAATA
    CCGAACAAGT CGCGGGCTGA AGAGCTGGCC ACTGCTCAGT TCAATGCCAC CAAGAAGGAG
    ATGAATGCAA ATGAATCGAG AGGCAACTTC TTCCGGCGAC GACGTTCGAC GCACCGACGC
    TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GATGTGGTCT TCTCCGATAG CATTTCCAAA
    TTCCCCGCCT ACGAGCGTGT CGTTCTGCGC CATCCAGGCT TTGTCCGACC CGTAGTGCTC
    TTCGGACCCG TATCCGATTT GGCCCGCGAG CGATTGGCCA AAGATTTCCC CGATAAGTTT
    TCGACTCCAC TGCAGGATGA CGACAAGTCA GCCGCAACTA GTGGAAAGTG CCGCATCGTA
    AGGCTCTCGA ATATACGCGA TGTGATGGAT CGTGGAAAGC ACGCCCTGCT GGACATTACG
    CCCAATGCAG TGGATCGTCT CAATTACGCG CAGTTCTATC CGGTGGTGAT CTTCCTGAAG
    ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGTCACGGAC TTCCCAAGGC GGCACACAAG
    AGCTCCAAGA AGCTGCTGGA GCAGTGCCAG AAACTGGAGC GCGTCTGGTC ACACATCTTT
    AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGATTCGATC
    GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCTA AGCCAGTAGA ATCCCTCTCC
    GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC
    GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG
    GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA
    GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGCTGTCCCC
    GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAT
    GTGCCACCAC AGCAGCAGCA GCCCGGCGTT GGAGGAGATA CGGCTGCAGT GAGACCGCAA
    TCTCTGTACG GGATTAATGC TCCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG
    GGCGATATAC CACTGAACAC CTCGGGCTCA TCGTCGCGAA ATGGCACCCT GGGTCGAAGT
    GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAGTACATA
    ACCCGAAACG CTCTGGTCGG GTCTGGAGCT GCAGAAACCC TGGATCGCCA GCAGCAGCAG
    CAGCAGCAGA CTCATGCATC CTTGGAGCGC CAAGCGCGTT TGAATGCGCA GCTAAAGGCC
    AACGGAGTAG GCGCCGGAGG AGGTGGTGCA TCCACCTACG ACAGTGTGTC CAGCTACGAC
    TCGTACAACA ATACACAAAT GGCCATGCAA AACCTGGGCC GACTAGGTCC CAATGCACCC
    GACGATCTCA AATCAGTGCC AAATGCAAAT GGTCGCCCCT TGCCTCCGAC GGGTCAGTCG
    CACGAGTACG GTCGCACTCC GCACGATCAT CGCAGCTTCG GAGGACCCAA CGATCTGAAC
    CGGCAGAGCA GTCCGGGCAG GCCGCACTAC CATGACATGA ATGCGTCCCG TAATATCGAT
    CCACGCAATG GCACACCACA GCGTCCTTCG AATCTGGGCC TGGAAAGCAG CCCACGCAAG
    CCTTTGGTGG AGACAAAAAC GGATTACGGC AAATACAGTC GGAATAACTC CGTAACGCAA
    GCGGATTATA CAAAGCTACC GAAGACTGCG CCACATGGAG TAGTTCCTCC GCCGAATGTG
    AGCAATGGCC AGGGCCAGAT GAATGGCAGC GGAACGCCGA GCAGCAATGG CAGTGGACCC
    TTCAAGCCGG TGCCACCACC CAAACCCAAA AACTATAGGC CACCGGTGCA AAGTGGTGGT
    AGCAGTGGCA GCGGAGGCAC AACTCCTTGG GAGAATGGGG ACTCTGGTTC GCCCCGTTCT
    CCGAATGGCT TCTATTATCC ACCAACGCCT TCGCATCATC ATTACGGCCA GCAGGCGACC
    CCGGGTTCAC CCAGCAATGG CCACATGCAG CCACCACCAC CGCAGCAGCA GCAGCCCACC
    TACGGCGGAA GTAATGGCAA CTATGGCCAG GCACCACCAC CACAATCCTA TCCGCAGGCC
    AATGGCTACA ATGGGAATGG GCATCACTAC AACGGGGGCA GCGGCACAGG ACCCTATATT
    GCACCACATC GCGGCATGCC ACCGCCAATA GGAAACCTGC CGCCTCACAC GCCCGAACGT
    CATGCTCTTG ACTTGGCCGG AAGTCGGGAG CAGCGCGGTT CGGCCTTCGA ACTGTATCGT
    AAACCGCAAA TCGGAGCTAC CGCCGGGCAC CATCACAACA TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04285
    Clone ID Related Accession (Same CDS sequence) NM_169245.3
    Accession Version NM_169245.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169245.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGTCGGAATA ACTCCGTAAC GCAAGCGGAT
    TATACAAAGC TACCGAAGAC TGCGCCACAT GGAGTAGTTC CTCCGCCGAA TGTGAGCAAT
    GGCCAGGGCC AGATGAATGG CAGCGGAACG CCGAGCAGCA ATGGCAGTGG ACCCTTCAAG
    CCGGTGCCAC CACCCAAACC CAAAAACTAT AGGCCACCGG TGCAAAGTGG TGGTAGCAGT
    GGCAGCGGAG GCACAACTCC TTGGGAGAAT GGGGACTCTG GTTCGCCCCG TTCTCCGAAT
    GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCATTACG GCCAGCAGGC GACCCCGGGT
    TCACCCAGCA ATGGCCACAT GCAGCCACCA CCACCGCAGC AGCAGCAGCC CACCTACGGC
    GGAAGTAATG GCAACTATGG CCAGGCACCA CCACCACAAT CCTATCCGCA GGCCAATGGC
    TACAATGGGA ATGGGCATCA CTACAACGGG GGCAGCGGCA CAGGACCCTA TATTGCACCA
    CATCGCGGCA TGCCACCGCC AATAGGAAAC CTGCCGCCTC ACACGCCCGA ACGTCATGCT
    CTTGACTTGG CCGGAAGTCG GGAGCAGCGC GGTTCGGCCT TCGAACTGTA TCGTAAACCG
    CAAATCGGAG CTACCGCCGG GCACCATCAC AACATGAGCG AAATGGAGCC GTACGATGAG
    CGCTACGATG ATTACTATAA CATGCCACCC CCTGCCGCCC ATCCGTCCCA AGGGCATCAT
    ATGCAGCGAT CCCGATCAGC TCCTCGTTAT CCACACGAGC GACCTCCTCA CGCCCAAGAT
    CCGAACTACT ACGGCCACTA TGGAACGTCC AGGGGACATA GTCAGCCGCG CCAGCAATAT
    TCGCAGCATC ACCAACAGCA GTATTACGAT GATCATGGGT TGGAGATGGG TCCACCGCCA
    TTGCCGCCAC ATAAGAAGAA GAAATCGGTG CTAAAGAGCC CACTGGTGGC ATTAAAGAAT
    GCACTGCTTA AGAGTACGAG GCCTCTAAGG AGAATGAACA GCATGGTGGA ACCGGAGAGG
    AAACCGAAGG GACTTCGACG CCAGCAATCG ATGCTGGAGA GGGGTGTGCA AAGACCCTAC
    TACCCGGATG AGTATCCAAC GTATCCAGCT GGCTTTGAGG AGCGTGCCCA TGGTGGACAA
    GGCATGATGC AACCACATTC ACAAGATGCC TACTATCAAA GGGGTGGTCA CTACGCGCCG
    CAACAGCAGG AGATGATGAA CAGCACCTAT CAGAATCTGG AGGGTGAGGA TATCTATGGG
    AATATCGGCA ACACAGTTCC CAGAATGCCG CATCACCAGG ACAATGGTTA CGGATATGAC
    CAATATGATC TCTATGCGAA TCGAGCGTGC ATCGATCTCG AAAGAAGGCA AGCAGAGGCA
    GCTGCAGCTT CTGGCGGACG AAATGGAAGG AGAATTGTAA GGCGGCACAG CACCACTACT
    GCAGATCGTG GAGGAAATCC CGGACCGCCA AGGAGGCCTA TGATCAGTCC TGGTTATGAA
    CAAGATCCGC AAGAGATCTA CCAGACCCGC AACGGAGCCT ACATGCTGCC CGATCAGCGA
    AGGGCGCCCA GTTCGGAGGT GATGACCCGT AGACGATTCT ATCCGGGGGC TGCCAATGAG
    CAGACTGAGG AGGAACCGCT CTACCAATCA CGACGCGAAA TGCAGCGCGA AATGCAGAGA
    AATCATCTGT ACCAATCGAA ACGAGAAATG CAGGAGAGGA TTAGCCAGGG CAAGAGGGAT
    ATGGAGCGAG AATTTAGCCC CCAAAGCAGC AGCAGCCAGT CGGAAGCCTC CAATTCGGAG
    GCAATATACC AGAGTCGGCG GGAGGCCAAG GAAAGTGCTC TGAAAACGAG GTCACAACTG
    AGGGATCAGA TCTATCAGAC TAGACGGGAA GCCCTGGACA GCATGGCTGA ACCGATCTAT
    GTATCCAAAA GAGGTATGGG CAGACCGGCC CCAATCTATG AGACCCGGGA AGAGAGTATC
    CTCCAGTCCC GAGAAAATGA AACAGATGAA AAGAAGGAAA AGGAGTGCAA AACCGAGCAG
    ATTCAGGTGG AGATTAATGC TCAACCGGAA GAACAGATCG AAGATTCGAC CTTAAGTCGT
    TCGGACCTGC AGAAATCTTC GGACACGGTG ATAGAAAATC CAGCTAGAGC TCCTCTTGTG
    CAGGACGAAG TAGATGATGA CGAAGAGGAG GAGGTTCAGG ATCAGCGTGA TCCCGCTGAG
    AGCTCTGCGG ATCAGACAGA GCAGCCAACA GCTATTGAAA ATAATCTGCA CAATGAAGGA
    ATGGTGGAGC AATCACAGAA TGATTCGGAT GACGTATTCG AAGCAGCAGA TAAGGTTTCT
    CCCCTGGCCC CTCCACCAGC TCCTGTTCAA CCGCCGACCC CGCGATCCGG TAGAGCTCCC
    TTCCACATTT CGAATATCCT TAAGCGAACA GCACCTCCGC CGAGTTCCCC AATCGGTGAC
    AGCTGTACCT CCATCGAAAC CCAGTACACA TCACAAGCCA GCTTGCCAGT GGGTCCACCC
    AATGCGACCA GTACGCCGTT TAGTAGCAGC ATGTCATTGC CCATCGCCGG ACCAGTAAAT
    AATCAACCTT CGGCCAACGG ACGCTTTCCC ACTCTGCCCC GTGAACCCAG CACCTCGCGC
    GGATTTTTTG ACTCCAACGG TGGCACTTTG GCGGATAAGC TGTGGCACGT CTCACTGCAA
    ATCCCGCCGG GAGCCATTCC GGCCGGAGTA CGACAAGAGA TCTACTTCAC CGTGAGCGAC
    CCGCGAATGG GACAAGCTGT CGGCGGTCCT CCGCTGGACA TGGAAAATGG TGAGACAATG
    CTGTCACCGT TGGTGATGTG CGGGCCGCAG GGCCTCGAGT TCCTCGTACC GGTTACGCTC
    AACATACCCC ACTGCGCCGG ACGAACTGCA TCACTGGGAC TGGCCCTCAA GGCGACCGAT
    AGCGAGAAGA ATCTGCACAC CGAGTGGGAC AACATCGACC TGCCCAGCAA TGCCGCCGCT
    CACACGGTCT CCGTCAAGGT GGATCACTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_731291.2
    CDS326..6598
    Translation

    Target ORF information:

    RefSeq Version NM_169245.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169245.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACATGGGAG 
    TACCACACGG TGGCGGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT
    GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG
    AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC
    GTCTCTCTGG AGAACGTGGA ATATGCTACC GCGGTGCAGG TGTTACGCGA TAGTGGCAAC
    ACGGTGCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATTAATGC TGCAGGTGCA
    GTGCAGCATC AGCATTCACA CAGTCTCAGT TCGGTTGGTC TAATGGCCAA TGGAAGTGGA
    GGAGTGGCGC CAACCCCCAT TACGAGTCTG AGTCAACCCA ACTCGCTGAA CTCTTCCCTA
    GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACCAAAGG CGGAAAGAAA
    GATGACTATG GTGTGGTGCT CGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCTAAAGCT
    CGCGAGCAAC TAAATGCCAA TGGTTATAGC TTGCAAGAGG GTGACATCAT CACCCGCATC
    CACAATACTA ACTGCGGGGA TACGATGAGT CTTAAGGAGG CGAAAAAGAT CATTGATGGC
    TGCAAGGAGC GATTGAACCT AGTGGTTCTA AGGGACATTA CCAACCAAAC AGCTGTTAGC
    CAATTGAATC TGAACAATTC AGCCAGCCAC CAGGCGTCGG GAAATATATA CGCCACGCAT
    CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG
    GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CGCCAACTCG CACCTCCAAT
    GGTCCCAATA TCAATGGCAA TGGCCTGAAC GACGAAAAGA GTAATCTTAC GCCGCGAGGT
    CGCTCCCGGG GCCCCATAAT GGATGGGGTA TCCCTGCAGC AACTGGATAG ACCCGTAACA
    CCGACTCGGG GCAGGAGTGC AGCCATCGAC GAGCCGCCGC GTCCACCACC ACCAAGGGGA
    TCGAGCGGAG GAGCAGCTCA AGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG
    CGTCAGAGTG CCGAACCGAG ATTCATTTCC TTCCAGAAGG AAGGAAGCGT AGGCATCCGG
    CTAACCGGCG GCAATGAAGC TGGTATTTTT GTGACTGCCG TACAACCAGG ATCTCCAGCC
    TCGCTGCAGG GTCTAATGCC TGGCGATAAG ATCCTCAAGG TCAACGACAT GGACATGAAC
    GGAGTGACGC GCGAGGAGGC TGTTCTCTTC CTGTTGAGTC TCCAGGATCG CATCGATCTG
    ATTGTGCAGT ACTGCAAGGA GGAATACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC
    TTCCACATCA AGACACATTT CCACTGCGAT AATCCTTCCA AAGGCGAGAT GGCCTTCAAG
    GCGGGCGATG TTTTCCGGGT GATCGATACC CTGCACAATG GTGTGGTCGG ATCCTGGCAG
    GTGCTGAAGA TCGGTCGGGG TCACCAGGAG ATGCAGCGCG GCGTAATACC GAACAAGTCG
    CGGGCTGAAG AGCTGGCCAC TGCTCAGTTC AATGCCACCA AGAAGGAGAT GAATGCAAAT
    GAATCGAGAG GCAACTTCTT CCGGCGACGA CGTTCGACGC ACCGACGCTC GAAGAGTCTG
    TCCCGCGAGA ACTGGGACGA TGTGGTCTTC TCCGATAGCA TTTCCAAATT CCCCGCCTAC
    GAGCGTGTCG TTCTGCGCCA TCCAGGCTTT GTCCGACCCG TAGTGCTCTT CGGACCCGTA
    TCCGATTTGG CCCGCGAGCG ATTGGCCAAA GATTTCCCCG ATAAGTTTTC GACTCCACTG
    CAGGATGACG ACAAGTCAGC CGCAACTAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAT
    ATACGCGATG TGATGGATCG TGGAAAGCAC GCCCTGCTGG ACATTACGCC CAATGCAGTG
    GATCGTCTCA ATTACGCGCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG
    CACGTGATCA AGCAGCTGCG TCACGGACTT CCCAAGGCGG CACACAAGAG CTCCAAGAAG
    CTGCTGGAGC AGTGCCAGAA ACTGGAGCGC GTCTGGTCAC ACATCTTTAG CACCCAGATC
    GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ATTCGATCGA TCTGCAGCAG
    AGCGGCGCCG TGTGGATGTC CGAGTCTAAG GTACCCTACG ACCATGCTGT CCCCGCGAAT
    CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAATGTGCCA
    CCACAGCAGC AGCAGCCCGG CGTTGGAGGA GATACGGCTG CAGTGAGACC GCAATCTCTG
    TACGGGATTA ATGCTCCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGCGAT
    ATACCACTGA ACACCTCGGG CTCATCGTCG CGAAATGGCA CCCTGGGTCG AAGTGCCCAG
    GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAGTA CATAACCCGA
    AACGCTCTGG TCGGGTCTGG AGCTGCAGAA ACCCTGGATC GCCAGCAGCA GCAGCAGCAG
    CAGACTCATG CATCCTTGGA GCGCCAAGCG CGTTTGAATG CGCAGCTAAA GGCCAACGGA
    GTAGGCGCCG GAGGAGGTGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC
    AACAATACAC AAATGGCCAT GCAAAACCTG GGCCGACTAG GTCCCAATGC ACCCGACGAT
    CTCAAATCAG TGCCAAATGC AAATGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCACGAG
    TACGGTCGCA CTCCGCACGA TCATCGCAGC TTCGGAGGAC CCAACGATCT GAACCGGCAG
    AGCAGTCCGG GCAGGCCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT CGATCCACGC
    AATGGCACAC CACAGCGTCC TTCGAATCTG GGCCTGGAAA GCAGCCCACG CAAGCCTTTG
    GTGGAGACAA AAACGGATTA CGGCAAATAC AGTCGGAATA ACTCCGTAAC GCAAGCGGAT
    TATACAAAGC TACCGAAGAC TGCGCCACAT GGAGTAGTTC CTCCGCCGAA TGTGAGCAAT
    GGCCAGGGCC AGATGAATGG CAGCGGAACG CCGAGCAGCA ATGGCAGTGG ACCCTTCAAG
    CCGGTGCCAC CACCCAAACC CAAAAACTAT AGGCCACCGG TGCAAAGTGG TGGTAGCAGT
    GGCAGCGGAG GCACAACTCC TTGGGAGAAT GGGGACTCTG GTTCGCCCCG TTCTCCGAAT
    GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCATTACG GCCAGCAGGC GACCCCGGGT
    TCACCCAGCA ATGGCCACAT GCAGCCACCA CCACCGCAGC AGCAGCAGCC CACCTACGGC
    GGAAGTAATG GCAACTATGG CCAGGCACCA CCACCACAAT CCTATCCGCA GGCCAATGGC
    TACAATGGGA ATGGGCATCA CTACAACGGG GGCAGCGGCA CAGGACCCTA TATTGCACCA
    CATCGCGGCA TGCCACCGCC AATAGGAAAC CTGCCGCCTC ACACGCCCGA ACGTCATGCT
    CTTGACTTGG CCGGAAGTCG GGAGCAGCGC GGTTCGGCCT TCGAACTGTA TCGTAAACCG
    CAAATCGGAG CTACCGCCGG GCACCATCAC AACATGAGCG AAATGGAGCC GTACGATGAG
    CGCTACGATG ATTACTATAA CATGCCACCC CCTGCCGCCC ATCCGTCCCA AGGGCATCAT
    ATGCAGCGAT CCCGATCAGC TCCTCGTTAT CCACACGAGC GACCTCCTCA CGCCCAAGAT
    CCGAACTACT ACGGCCACTA TGGAACGTCC AGGGGACATA GTCAGCCGCG CCAGCAATAT
    TCGCAGCATC ACCAACAGCA GTATTACGAT GATCATGGGT TGGAGATGGG TCCACCGCCA
    TTGCCGCCAC ATAAGAAGAA GAAATCGGTG CTAAAGAGCC CACTGGTGGC ATTAAAGAAT
    GCACTGCTTA AGAGTACGAG GCCTCTAAGG AGAATGAACA GCATGGTGGA ACCGGAGAGG
    AAACCGAAGG GACTTCGACG CCAGCAATCG ATGCTGGAGA GGGGTGTGCA AAGACCCTAC
    TACCCGGATG AGTATCCAAC GTATCCAGCT GGCTTTGAGG AGCGTGCCCA TGGTGGACAA
    GGCATGATGC AACCACATTC ACAAGATGCC TACTATCAAA GGGGTGGTCA CTACGCGCCG
    CAACAGCAGG AGATGATGAA CAGCACCTAT CAGAATCTGG AGGGTGAGGA TATCTATGGG
    AATATCGGCA ACACAGTTCC CAGAATGCCG CATCACCAGG ACAATGGTTA CGGATATGAC
    CAATATGATC TCTATGCGAA TCGAGCGTGC ATCGATCTCG AAAGAAGGCA AGCAGAGGCA
    GCTGCAGCTT CTGGCGGACG AAATGGAAGG AGAATTGTAA GGCGGCACAG CACCACTACT
    GCAGATCGTG GAGGAAATCC CGGACCGCCA AGGAGGCCTA TGATCAGTCC TGGTTATGAA
    CAAGATCCGC AAGAGATCTA CCAGACCCGC AACGGAGCCT ACATGCTGCC CGATCAGCGA
    AGGGCGCCCA GTTCGGAGGT GATGACCCGT AGACGATTCT ATCCGGGGGC TGCCAATGAG
    CAGACTGAGG AGGAACCGCT CTACCAATCA CGACGCGAAA TGCAGCGCGA AATGCAGAGA
    AATCATCTGT ACCAATCGAA ACGAGAAATG CAGGAGAGGA TTAGCCAGGG CAAGAGGGAT
    ATGGAGCGAG AATTTAGCCC CCAAAGCAGC AGCAGCCAGT CGGAAGCCTC CAATTCGGAG
    GCAATATACC AGAGTCGGCG GGAGGCCAAG GAAAGTGCTC TGAAAACGAG GTCACAACTG
    AGGGATCAGA TCTATCAGAC TAGACGGGAA GCCCTGGACA GCATGGCTGA ACCGATCTAT
    GTATCCAAAA GAGGTATGGG CAGACCGGCC CCAATCTATG AGACCCGGGA AGAGAGTATC
    CTCCAGTCCC GAGAAAATGA AACAGATGAA AAGAAGGAAA AGGAGTGCAA AACCGAGCAG
    ATTCAGGTGG AGATTAATGC TCAACCGGAA GAACAGATCG AAGATTCGAC CTTAAGTCGT
    TCGGACCTGC AGAAATCTTC GGACACGGTG ATAGAAAATC CAGCTAGAGC TCCTCTTGTG
    CAGGACGAAG TAGATGATGA CGAAGAGGAG GAGGTTCAGG ATCAGCGTGA TCCCGCTGAG
    AGCTCTGCGG ATCAGACAGA GCAGCCAACA GCTATTGAAA ATAATCTGCA CAATGAAGGA
    ATGGTGGAGC AATCACAGAA TGATTCGGAT GACGTATTCG AAGCAGCAGA TAAGGTTTCT
    CCCCTGGCCC CTCCACCAGC TCCTGTTCAA CCGCCGACCC CGCGATCCGG TAGAGCTCCC
    TTCCACATTT CGAATATCCT TAAGCGAACA GCACCTCCGC CGAGTTCCCC AATCGGTGAC
    AGCTGTACCT CCATCGAAAC CCAGTACACA TCACAAGCCA GCTTGCCAGT GGGTCCACCC
    AATGCGACCA GTACGCCGTT TAGTAGCAGC ATGTCATTGC CCATCGCCGG ACCAGTAAAT
    AATCAACCTT CGGCCAACGG ACGCTTTCCC ACTCTGCCCC GTGAACCCAG CACCTCGCGC
    GGATTTTTTG ACTCCAACGG TGGCACTTTG GCGGATAAGC TGTGGCACGT CTCACTGCAA
    ATCCCGCCGG GAGCCATTCC GGCCGGAGTA CGACAAGAGA TCTACTTCAC CGTGAGCGAC
    CCGCGAATGG GACAAGCTGT CGGCGGTCCT CCGCTGGACA TGGAAAATGG TGAGACAATG
    CTGTCACCGT TGGTGATGTG CGGGCCGCAG GGCCTCGAGT TCCTCGTACC GGTTACGCTC
    AACATACCCC ACTGCGCCGG ACGAACTGCA TCACTGGGAC TGGCCCTCAA GGCGACCGAT
    AGCGAGAAGA ATCTGCACAC CGAGTGGGAC AACATCGACC TGCCCAGCAA TGCCGCCGCT
    CACACGGTCT CCGTCAAGGT GGATCACTTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04288
    Clone ID Related Accession (Same CDS sequence) NM_001104242.3
    Accession Version NM_001104242.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104242.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGCAAT TCGACTATGC CTGCTATACG GTGCCCGAGT GTTCCACGGA TGATGATGAT 
    GATAATGACG ATGAGCTGGG CAGTAATGTG ACCCTGGAGA ACAAGGATCA TTCGGATTGC
    ACCCTGGAAT CGGATGCTAC GACCACCACA TCTTCACTGG AGCGTCATAT GTATAGGAAT
    CGGTATCGCT CTAATAGAAG TTTCGGACCA CAGAGGGTTT CCATGCCCAT TCTCAGATCC
    AGTCGATCCA GGGAGATGAC GCCCCAGTTG GGCATGTATC ATGAGGGACC GAATCGAGAT
    CCCCAATGGG GTTACTTTGT ACCCCTGTCG CCCAACTTCT TCTTTAGTCC ACCGATGCAG
    AGGAGGCGTT ATAGGGAAAA GGAAAACGAT CGCAATCGTC GCGCTGTTAG TTATGGCGGT
    GACGCCACCT CCTCCCCCCA TTCGGCCACC ATACCCCGCG CCGCCCCCCG TCGGGTGGCC
    TTGATGAAGG ATCCACCGCC ACCGCCGCCA CCCAAGCCGC AATTGGGTTC CAAAGCGGAT
    TCTGTATCGC ATTTGGCCAG CAAATATCCC CAATCCGAGT ACAATCTCAT ACAGAAAATC
    GACTCGAACA GCACACTCAC TGCTCCGGCG CAATATCAAC CGCAGAATTT CTACGCTAAT
    ACTGGCACCA TATCCTCTAC GAATGGCTAC GGATCGCTGT TATGTCACGC CAGTTCGACG
    ACCAGTCCGC TGGCGGTCCG GAAGAGGGAC AAACTGCTTC ATCGCTTCAG CGATGCCGCC
    ACCTTGGGCC GGAAGCTCAA GAAGAAGAAG AACACGAATC GCACCTGCAG ATCCATGACG
    GAGGCCATTG AGATGCTGGC CGATCCCGTC ATCGAAGATG AATTCTTTGG TGATCGAACC
    ACATGGGAGT ACCACACGGT GGCGGTGACT CGGGTGCCGG GCTATGGATT CGGCATCGCC
    GTTTCCGGTG GACGTGATAA TCCGCACTTC GCCAACGGCG ATCCCTCGAT AGCAGTTAGC
    GATGTGCTGA AAGGTGGCCC CGCCGAGGAT CGATTGCAAG TCAACGATCG GATAATCTCG
    GTGAACGGCG TCTCTCTGGA GAACGTGGAA TATGCTACCG CGGTGCAGGT GTTACGCGAT
    AGTGGCAACA CGGTGCAATT GGTGGTCAAG CGTCGCGTGC CCCTCAATCC CATTAATGCT
    GCAGGTGCAG TGCAGCATCA GCATTCACAC AGTCTCAGTT CGGTTGGTCT AATGGCCAAT
    GGAAGTGGAG GAGTGGCGCC AACCCCCATT ACGAGTCTGA GTCAACCCAA CTCGCTGAAC
    TCTTCCCTAG TGCAGAATGC CAGCAGTGGT CAGCCCATCA AGGTGACCCT CACCAAAGGC
    GGAAAGAAAG ATGACTATGG TGTGGTGCTC GGCTGCCGGC TGTTTGTTAA GGAGATCTCC
    TCTAAAGCTC GCGAGCAACT AAATGCCAAT GGTTATAGCT TGCAAGAGGG TGACATCATC
    ACCCGCATCC ACAATACTAA CTGCGGGGAT ACGATGAGTC TTAAGGAGGC GAAAAAGATC
    ATTGATGGCT GCAAGGAGCG ATTGAACCTA GTGGTTCTAA GGGACATTAC CAACCAAACA
    GCTGTTAGCC AATTGAATCT GAACAATTCA GCCAGCCACC AGGCGTCGGG AAATATATAC
    GCCACGCATC AGCCTCAGGT ATCCGGATGT AGCAGCAGTA ACAACAATCT TGAAGATCCA
    TATCTGCCGG GCGGAGCCAG TTACTCCTCG CAGAATCTGT ACGTGCAACC GCCAACTCGC
    ACCTCCAATG GTCCCAATAT CAATGGCAAT GGCCTGAACG ACGAAAAGAG TAATCTTACG
    CCGCGAGGTC GCTCCCGGGG CCCCATAATG GATGGGGTAT CCCTGCAGCA ACTGGATAGA
    CCCGTAACAC CGACTCGGGG CAGGAGTGCA GCCATCGACG AGCCGCCGCG TCCACCACCA
    CCAAGGGGAT CGAGCGGAGG AGCAGCTCAA GAGGATTTCT ACAGCTCTCG CAGGCAATTG
    TACGAGGAGC GTCAGAGTGC CGAACCGAGA TTCATTTCCT TCCAGAAGGA AGGAAGCGTA
    GGCATCCGGC TAACCGGCGG CAATGAAGCT GGTATTTTTG TGACTGCCGT ACAACCAGGA
    TCTCCAGCCT CGCTGCAGGG TCTAATGCCT GGCGATAAGA TCCTCAAGGT CAACGACATG
    GACATGAACG GAGTGACGCG CGAGGAGGCT GTTCTCTTCC TGTTGAGTCT CCAGGATCGC
    ATCGATCTGA TTGTGCAGTA CTGCAAGGAG GAATACGACG AGGTGGTGAC CAACCAACGT
    GGCGACTCCT TCCACATCAA GACACATTTC CACTGCGATA ATCCTTCCAA AGGCGAGATG
    GCCTTCAAGG CGGGCGATGT TTTCCGGGTG ATCGATACCC TGCACAATGG TGTGGTCGGA
    TCCTGGCAGG TGCTGAAGAT CGGTCGGGGT CACCAGGAGA TGCAGCGCGG CGTAATACCG
    AACAAGTCGC GGGCTGAAGA GCTGGCCACT GCTCAGTTCA ATGCCACCAA GAAGGAGATG
    AATGCAAATG AATCGAGAGG CAACTTCTTC CGGCGACGAC GTTCGACGCA CCGACGCTCG
    AAGAGTCTGT CCCGCGAGAA CTGGGACGAT GTGGTCTTCT CCGATAGCAT TTCCAAATTC
    CCCGCCTACG AGCGTGTCGT TCTGCGCCAT CCAGGCTTTG TCCGACCCGT AGTGCTCTTC
    GGACCCGTAT CCGATTTGGC CCGCGAGCGA TTGGCCAAAG ATTTCCCCGA TAAGTTTTCG
    ACTCCACTGC AGGATGACGA CAAGTCAGCC GCAACTAGTG GAAAGTGCCG CATCGTAAGG
    CTCTCGAATA TACGCGATGT GATGGATCGT GGAAAGCACG CCCTGCTGGA CATTACGCCC
    AATGCAGTGG ATCGTCTCAA TTACGCGCAG TTCTATCCGG TGGTGATCTT CCTGAAGACA
    GACAGCAAGC ACGTGATCAA GCAGCTGCGT CACGGACTTC CCAAGGCGGC ACACAAGAGC
    TCCAAGAAGC TGCTGGAGCA GTGCCAGAAA CTGGAGCGCG TCTGGTCACA CATCTTTAGC
    ACCCAGATCG CGTTGAGCGA CGAGGAGTCC TGGTACCGAA AGTTGCGCGA TTCGATCGAT
    CTGCAGCAGA GCGGCGCCGT GTGGATGTCC GAGTCTAAGT TCCCCGATTC CCCCACCGAA
    ATGTTATTCC CCCCCTACAT CGCCCAGCCA TGCAAGTTAG CTTGTTGCCG CCCCATATCA
    TCTACCTCAG TGCCACGCTA CTCGCAGGAT ATGAATCTGA ATCTAAACCA GAATCCAAGT
    AAATCCTATT CACTAATCCA GAACCAGGCA CATACCCCTC CGCCGCCACG CCCACCGCCC
    GTGGGTTATG CCCACAGCCA GAGTTTGGCC GAAAAGCCCC GAGATCGCAG GTCTTATACG
    ATGGTGCCGA TCATAAAGCC CGCTTCTTCG ATGCCCAGTG GTCTGGCACC GACCGTTGAG
    CCTGCTGCTC CCATGATGCC ACTAGACGAT TGTGTTCTGG CCATGGATGG GTCCAACTCC
    CAGTATCATA GTTCTCAGCC AAATCTAATG AGTCAGAGTT ATTTGAACTC GAATTTAAAT
    GGCGGCAGTA CGAGTGCCGT GGATCAAACG AATCACTCAA ACGCCATGAG GGGCTTTCAG
    CCCGGACCAT CGGTAGTCAG CCGCTATGGA TTGCGGTCCA ATGCCCAGGT GGAACGTAGG
    ACCACTCCCT ACTACTACCA CGAGCTGCTG CTGCAGAAGA ATCTCTTCGA TTCCAGTCTG
    AATCTTCTGC CCCAGCAGCA GCAGCAGCAT CAGCCGGATC TGGCCAAGAG CAAGAACAGC
    AATGTGCAGC TGGAGCAGCA ACAACAGCAG CAGCAGCAGC ATCAGAAGCA TCTTGCAGAT
    GCATCGAATG CATCCAAAAG GGCACCGATG GCCAAGCCAC AGCAGCGTTT GATTAGCAAT
    GGTCAGGGCA GGAGCAACTA CTCCTCCTCG GGGTCACTGA GCAATCTTCT GGGCAATGGC
    CATGGGCTGA GCAATGGTCA CGAGAGCAGC GATGGCACTT TGGTGGATGA GGGCAGCTTT
    ACACTGGCCT TCAATAATCT CACCAATCAA ATAGCCAGTC TCAATAATTC AAGTAGTCTA
    AACAATAATA ATTGTAGCAC CAGCAGGAGC ATCAACAACA ACAGCAACAG CCATAATATT
    AATGCCAACA ACAACAATAG CCATGTTAAT AATGCTGATT CCAACAACAA TGTTGGCCAA
    AGTAACGAAT GCATTGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097712.1
    CDS412..4752
    Translation

    Target ORF information:

    RefSeq Version NM_001104242.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001104242.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCGCAAT TCGACTATGC CTGCTATACG GTGCCCGAGT GTTCCACGGA TGATGATGAT 
    GATAATGACG ATGAGCTGGG CAGTAATGTG ACCCTGGAGA ACAAGGATCA TTCGGATTGC
    ACCCTGGAAT CGGATGCTAC GACCACCACA TCTTCACTGG AGCGTCATAT GTATAGGAAT
    CGGTATCGCT CTAATAGAAG TTTCGGACCA CAGAGGGTTT CCATGCCCAT TCTCAGATCC
    AGTCGATCCA GGGAGATGAC GCCCCAGTTG GGCATGTATC ATGAGGGACC GAATCGAGAT
    CCCCAATGGG GTTACTTTGT ACCCCTGTCG CCCAACTTCT TCTTTAGTCC ACCGATGCAG
    AGGAGGCGTT ATAGGGAAAA GGAAAACGAT CGCAATCGTC GCGCTGTTAG TTATGGCGGT
    GACGCCACCT CCTCCCCCCA TTCGGCCACC ATACCCCGCG CCGCCCCCCG TCGGGTGGCC
    TTGATGAAGG ATCCACCGCC ACCGCCGCCA CCCAAGCCGC AATTGGGTTC CAAAGCGGAT
    TCTGTATCGC ATTTGGCCAG CAAATATCCC CAATCCGAGT ACAATCTCAT ACAGAAAATC
    GACTCGAACA GCACACTCAC TGCTCCGGCG CAATATCAAC CGCAGAATTT CTACGCTAAT
    ACTGGCACCA TATCCTCTAC GAATGGCTAC GGATCGCTGT TATGTCACGC CAGTTCGACG
    ACCAGTCCGC TGGCGGTCCG GAAGAGGGAC AAACTGCTTC ATCGCTTCAG CGATGCCGCC
    ACCTTGGGCC GGAAGCTCAA GAAGAAGAAG AACACGAATC GCACCTGCAG ATCCATGACG
    GAGGCCATTG AGATGCTGGC CGATCCCGTC ATCGAAGATG AATTCTTTGG TGATCGAACC
    ACATGGGAGT ACCACACGGT GGCGGTGACT CGGGTGCCGG GCTATGGATT CGGCATCGCC
    GTTTCCGGTG GACGTGATAA TCCGCACTTC GCCAACGGCG ATCCCTCGAT AGCAGTTAGC
    GATGTGCTGA AAGGTGGCCC CGCCGAGGAT CGATTGCAAG TCAACGATCG GATAATCTCG
    GTGAACGGCG TCTCTCTGGA GAACGTGGAA TATGCTACCG CGGTGCAGGT GTTACGCGAT
    AGTGGCAACA CGGTGCAATT GGTGGTCAAG CGTCGCGTGC CCCTCAATCC CATTAATGCT
    GCAGGTGCAG TGCAGCATCA GCATTCACAC AGTCTCAGTT CGGTTGGTCT AATGGCCAAT
    GGAAGTGGAG GAGTGGCGCC AACCCCCATT ACGAGTCTGA GTCAACCCAA CTCGCTGAAC
    TCTTCCCTAG TGCAGAATGC CAGCAGTGGT CAGCCCATCA AGGTGACCCT CACCAAAGGC
    GGAAAGAAAG ATGACTATGG TGTGGTGCTC GGCTGCCGGC TGTTTGTTAA GGAGATCTCC
    TCTAAAGCTC GCGAGCAACT AAATGCCAAT GGTTATAGCT TGCAAGAGGG TGACATCATC
    ACCCGCATCC ACAATACTAA CTGCGGGGAT ACGATGAGTC TTAAGGAGGC GAAAAAGATC
    ATTGATGGCT GCAAGGAGCG ATTGAACCTA GTGGTTCTAA GGGACATTAC CAACCAAACA
    GCTGTTAGCC AATTGAATCT GAACAATTCA GCCAGCCACC AGGCGTCGGG AAATATATAC
    GCCACGCATC AGCCTCAGGT ATCCGGATGT AGCAGCAGTA ACAACAATCT TGAAGATCCA
    TATCTGCCGG GCGGAGCCAG TTACTCCTCG CAGAATCTGT ACGTGCAACC GCCAACTCGC
    ACCTCCAATG GTCCCAATAT CAATGGCAAT GGCCTGAACG ACGAAAAGAG TAATCTTACG
    CCGCGAGGTC GCTCCCGGGG CCCCATAATG GATGGGGTAT CCCTGCAGCA ACTGGATAGA
    CCCGTAACAC CGACTCGGGG CAGGAGTGCA GCCATCGACG AGCCGCCGCG TCCACCACCA
    CCAAGGGGAT CGAGCGGAGG AGCAGCTCAA GAGGATTTCT ACAGCTCTCG CAGGCAATTG
    TACGAGGAGC GTCAGAGTGC CGAACCGAGA TTCATTTCCT TCCAGAAGGA AGGAAGCGTA
    GGCATCCGGC TAACCGGCGG CAATGAAGCT GGTATTTTTG TGACTGCCGT ACAACCAGGA
    TCTCCAGCCT CGCTGCAGGG TCTAATGCCT GGCGATAAGA TCCTCAAGGT CAACGACATG
    GACATGAACG GAGTGACGCG CGAGGAGGCT GTTCTCTTCC TGTTGAGTCT CCAGGATCGC
    ATCGATCTGA TTGTGCAGTA CTGCAAGGAG GAATACGACG AGGTGGTGAC CAACCAACGT
    GGCGACTCCT TCCACATCAA GACACATTTC CACTGCGATA ATCCTTCCAA AGGCGAGATG
    GCCTTCAAGG CGGGCGATGT TTTCCGGGTG ATCGATACCC TGCACAATGG TGTGGTCGGA
    TCCTGGCAGG TGCTGAAGAT CGGTCGGGGT CACCAGGAGA TGCAGCGCGG CGTAATACCG
    AACAAGTCGC GGGCTGAAGA GCTGGCCACT GCTCAGTTCA ATGCCACCAA GAAGGAGATG
    AATGCAAATG AATCGAGAGG CAACTTCTTC CGGCGACGAC GTTCGACGCA CCGACGCTCG
    AAGAGTCTGT CCCGCGAGAA CTGGGACGAT GTGGTCTTCT CCGATAGCAT TTCCAAATTC
    CCCGCCTACG AGCGTGTCGT TCTGCGCCAT CCAGGCTTTG TCCGACCCGT AGTGCTCTTC
    GGACCCGTAT CCGATTTGGC CCGCGAGCGA TTGGCCAAAG ATTTCCCCGA TAAGTTTTCG
    ACTCCACTGC AGGATGACGA CAAGTCAGCC GCAACTAGTG GAAAGTGCCG CATCGTAAGG
    CTCTCGAATA TACGCGATGT GATGGATCGT GGAAAGCACG CCCTGCTGGA CATTACGCCC
    AATGCAGTGG ATCGTCTCAA TTACGCGCAG TTCTATCCGG TGGTGATCTT CCTGAAGACA
    GACAGCAAGC ACGTGATCAA GCAGCTGCGT CACGGACTTC CCAAGGCGGC ACACAAGAGC
    TCCAAGAAGC TGCTGGAGCA GTGCCAGAAA CTGGAGCGCG TCTGGTCACA CATCTTTAGC
    ACCCAGATCG CGTTGAGCGA CGAGGAGTCC TGGTACCGAA AGTTGCGCGA TTCGATCGAT
    CTGCAGCAGA GCGGCGCCGT GTGGATGTCC GAGTCTAAGT TCCCCGATTC CCCCACCGAA
    ATGTTATTCC CCCCCTACAT CGCCCAGCCA TGCAAGTTAG CTTGTTGCCG CCCCATATCA
    TCTACCTCAG TGCCACGCTA CTCGCAGGAT ATGAATCTGA ATCTAAACCA GAATCCAAGT
    AAATCCTATT CACTAATCCA GAACCAGGCA CATACCCCTC CGCCGCCACG CCCACCGCCC
    GTGGGTTATG CCCACAGCCA GAGTTTGGCC GAAAAGCCCC GAGATCGCAG GTCTTATACG
    ATGGTGCCGA TCATAAAGCC CGCTTCTTCG ATGCCCAGTG GTCTGGCACC GACCGTTGAG
    CCTGCTGCTC CCATGATGCC ACTAGACGAT TGTGTTCTGG CCATGGATGG GTCCAACTCC
    CAGTATCATA GTTCTCAGCC AAATCTAATG AGTCAGAGTT ATTTGAACTC GAATTTAAAT
    GGCGGCAGTA CGAGTGCCGT GGATCAAACG AATCACTCAA ACGCCATGAG GGGCTTTCAG
    CCCGGACCAT CGGTAGTCAG CCGCTATGGA TTGCGGTCCA ATGCCCAGGT GGAACGTAGG
    ACCACTCCCT ACTACTACCA CGAGCTGCTG CTGCAGAAGA ATCTCTTCGA TTCCAGTCTG
    AATCTTCTGC CCCAGCAGCA GCAGCAGCAT CAGCCGGATC TGGCCAAGAG CAAGAACAGC
    AATGTGCAGC TGGAGCAGCA ACAACAGCAG CAGCAGCAGC ATCAGAAGCA TCTTGCAGAT
    GCATCGAATG CATCCAAAAG GGCACCGATG GCCAAGCCAC AGCAGCGTTT GATTAGCAAT
    GGTCAGGGCA GGAGCAACTA CTCCTCCTCG GGGTCACTGA GCAATCTTCT GGGCAATGGC
    CATGGGCTGA GCAATGGTCA CGAGAGCAGC GATGGCACTT TGGTGGATGA GGGCAGCTTT
    ACACTGGCCT TCAATAATCT CACCAATCAA ATAGCCAGTC TCAATAATTC AAGTAGTCTA
    AACAATAATA ATTGTAGCAC CAGCAGGAGC ATCAACAACA ACAGCAACAG CCATAATATT
    AATGCCAACA ACAACAATAG CCATGTTAAT AATGCTGATT CCAACAACAA TGTTGGCCAA
    AGTAACGAAT GCATTGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07908
    Clone ID Related Accession (Same CDS sequence) NM_001260069.2
    Accession Version NM_001260069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260069.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAAGCCCA GCACGCGACG TCGCAGTTTC AAAACGAAAC CCCCCAAATC GAATGTATAT 
    AACCCACTCG TTCGCTATCG CCCGACAGCC TATGTCCGAA ATGGAATTAT TGGCGGCGCC
    GAGGGTCCCG GCTCGAGTGG AGTGTACCAA ATCGCGGATA GGGTCTATAC CACCAGCAAG
    ATGTTTCTAC GCGATGGCTA TATGTACCCG CGTCAACCGG GTCAGACCAT GGATCCCCGT
    TTCCCCCTCG AGTTGCACGA ATTCCCTCAG CAATTCGGCG CCACTGGCCT GCAGTTGGTT
    AGTGCACCCA AGGTTCATAT AGCCGGCGAA TATCTGGCCA GTGAACAACA GCAGCAGCAG
    CAACAACAGC AGCAGCAGCA TCAACAGGTG CAGGAGCAAC AACAATTGGG CAGAAGAAAG
    GCTGCGGCAG CCGCCGAAAT AAATTTGGCA AATCAGGTTT ACTGGCTGGG CAAGCAAAAG
    ACCAGATCCC GATCGCGTAG CAATAGTGTG GGCAATCGGA GCATAACCAG CTGCATGGGC
    GGCGGTGGAG GGGGTGGCGA AAAGGAAAGG GAACGCGGCG GGGATCGAGA TCGCCCCTAT
    ATACGCAACG TTGACGATCT TCTCCAGGCA CTGGGCAAAA GGGATGGGGG GGATAACAAC
    AGCAATCTGC TCGAAACTGC ATCGACGCAC TCCTCATCGC AGCGAAGGCG CTTCAAGAAG
    GGTGATCGAA CCACATGGGA GTACCACACG GTGGCGGTGA CTCGGGTGCC GGGCTATGGA
    TTCGGCATCG CCGTTTCCGG TGGACGTGAT AATCCGCACT TCGCCAACGG CGATCCCTCG
    ATAGCAGTTA GCGATGTGCT GAAAGGTGGC CCCGCCGAGG ATCGATTGCA AGTCAACGAT
    CGGATAATCT CGGTGAACGG CGTCTCTCTG GAGAACGTGG AATATGCTAC CGCGGTGCAG
    GTGTTACGCG ATAGTGGCAA CACGGTGCAA TTGGTGGTCA AGCGTCGCGT GCCCCTCAAT
    CCCATTAATG CTGCAGGTGC AGTGCAGCAT CAGCATTCAC ACAGTCTCAG TTCGGTTGGT
    CTAATGGCCA ATGGAAGTGG AGGAGTGGCG CCAACCCCCA TTACGAGTCT GAGTCAACCC
    AACTCGCTGA ACTCTTCCCT AGTGCAGAAT GCCAGCAGTG GTCAGCCCAT CAAGGTGACC
    CTCACCAAAG GCGGAAAGAA AGATGACTAT GGTGTGGTGC TCGGCTGCCG GCTGTTTGTT
    AAGGAGATCT CCTCTAAAGC TCGCGAGCAA CTAAATGCCA ATGGTTATAG CTTGCAAGAG
    GGTGACATCA TCACCCGCAT CCACAATACT AACTGCGGGG ATACGATGAG TCTTAAGGAG
    GCGAAAAAGA TCATTGATGG CTGCAAGGAG CGATTGAACC TAGTGGTTCT AAGGGACATT
    ACCAACCAAA CAGCTGTTAG CCAATTGAAT CTGAACAATT CAGCCAGCCA CCAGGCGTCG
    GGAAATATAT ACGCCACGCA TCAGCCTCAG GTATCCGGAT GTAGCAGCAG TAACAACAAT
    CTTGAAGATC CATATCTGCC GGGCGGAGCC AGTTACTCCT CGCAGAATCT GTACGTGCAA
    CCGCCAACTC GCACCTCCAA TGGTCCCAAT ATCAATGGCA ATGGCCTGAA CGACGAAAAG
    AGTAATCTTA CGCCGCGAGG TCGCTCCCGG GGCCCCATAA TGGATGGGGT ATCCCTGCAG
    CAACTGGATA GACCCGTAAC ACCGACTCGG GGCAGGAGTG CAGCCATCGA CGAGCCGCCG
    CGTCCACCAC CACCAAGGGG ATCGAGCGGA GGAGCAGCTC AAGAGGATTT CTACAGCTCT
    CGCAGGCAAT TGTACGAGGA GCGTCAGAGT GCCGAACCGA GATTCATTTC CTTCCAGAAG
    GAAGGAAGCG TAGGCATCCG GCTAACCGGC GGCAATGAAG CTGGTATTTT TGTGACTGCC
    GTACAACCAG GATCTCCAGC CTCGCTGCAG GGTCTAATGC CTGGCGATAA GATCCTCAAG
    GTCAACGACA TGGACATGAA CGGAGTGACG CGCGAGGAGG CTGTTCTCTT CCTGTTGAGT
    CTCCAGGATC GCATCGATCT GATTGTGCAG TACTGCAAGG AGGAATACGA CGAGGTGGTG
    ACCAACCAAC GTGGCGACTC CTTCCACATC AAGACACATT TCCACTGCGA TAATCCTTCC
    AAAGGCGAGA TGGCCTTCAA GGCGGGCGAT GTTTTCCGGG TGATCGATAC CCTGCACAAT
    GGTGTGGTCG GATCCTGGCA GGTGCTGAAG ATCGGTCGGG GTCACCAGGA GATGCAGCGC
    GGCGTAATAC CGAACAAGTC GCGGGCTGAA GAGCTGGCCA CTGCTCAGTT CAATGCCACC
    AAGAAGGAGA TGAATGCAAA TGAATCGAGA GGCAACTTCT TCCGGCGACG ACGTTCGACG
    CACCGACGCT CGAAGAGTCT GTCCCGCGAG AACTGGGACG ATGTGGTCTT CTCCGATAGC
    ATTTCCAAAT TCCCCGCCTA CGAGCGTGTC GTTCTGCGCC ATCCAGGCTT TGTCCGACCC
    GTAGTGCTCT TCGGACCCGT ATCCGATTTG GCCCGCGAGC GATTGGCCAA AGATTTCCCC
    GATAAGTTTT CGACTCCACT GCAGGATGAC GACAAGTCAG CCGCAACTAG TGGAAAGTGC
    CGCATCGTAA GGCTCTCGAA TATACGCGAT GTGATGGATC GTGGAAAGCA CGCCCTGCTG
    GACATTACGC CCAATGCAGT GGATCGTCTC AATTACGCGC AGTTCTATCC GGTGGTGATC
    TTCCTGAAGA CAGACAGCAA GCACGTGATC AAGCAGCTGC GTCACGGACT TCCCAAGGCG
    GCACACAAGA GCTCCAAGAA GCTGCTGGAG CAGTGCCAGA AACTGGAGCG CGTCTGGTCA
    CACATCTTTA GCACCCAGAT CGCGTTGAGC GACGAGGAGT CCTGGTACCG AAAGTTGCGC
    GATTCGATCG ATCTGCAGCA GAGCGGCGCC GTGTGGATGT CCGAGTCTAA GCCAGTAGAA
    TCCCTCTCCG ACGATTTCCT ATTCCCCATG ACCACATCCC GACTCTCGTA TGCATCAAGT
    CCCGAATCCG ATTTGGAACT GAGTCCCGGC CCATCCGCAT CATTGTCGCT TGGCAATTTG
    CCGCAGTTGG TTAAAGCGAG CTCAGATCCA TCGATTGCCA CTAATCAGGA TAACTTGGAT
    AGGGATAGAG ACATAATTGG TGAAGGACTA CCACCTCCAT ATACGGTACC CTACGACCAT
    GCTGTCCCCG CGAATCCCAA TCGCCGCCAG ACCATGGACT CCAGCAAGTA CAGCATCTAC
    GGCACCAATG TGCCACCACA GCAGCAGCAG CCCGGCGTTG GAGGAGATAC GGCTGCAGTG
    AGACCGCAAT CTCTGTACGG GATTAATGCT CCCGATCTGC CGCCCCGCAT CGATCGTCAG
    TCCAAGCCGG GCGATATACC ACTGAACACC TCGGGCTCAT CGTCGCGAAA TGGCACCCTG
    GGTCGAAGTG CCCAGGAGCG TCTGTTTGGC AAGGCTGTGG TGCAGGACGA TGTCCAGGCG
    GAGTACATAA CCCGAAACGC TCTGGTCGGG TCTGGAGCTG CAGAAACCCT GGATCGCCAG
    CAGCAGCAGC AGCAGCAGAC TCATGCATCC TTGGAGCGCC AAGCGCGTTT GAATGCGCAG
    CTAAAGGCCA ACGGAGTAGG CGCCGGAGGA GGTGGTGCAT CCACCTACGA CAGTGTGTCC
    AGCTACGACT CGTACAACAA TACACAAATG GCCATGCAAA ACCTGGGCCG ACTAGGTCCC
    AATGCACCCG ACGATCTCAA ATCAGTGCCA AATGCAAATG GTCGCCCCTT GCCTCCGACG
    GGTCAGTCGC ACGAGTACGG TCGCACTCCG CACGATCATC GCAGCTTCGG AGGACCCAAC
    GATCTGAACC GGCAGAGCAG TCCGGGCAGG CCGCACTACC ATGACATGAA TGCGTCCCGT
    AATATCGATC CACGCAATGG CACACCACAG CGTCCTTCGA ATCTGGGCCT GGAAAGCAGC
    CCACGCAAGC CTTTGGTGGA GACAAAAACG GATTACGGCA AATACAGTCG GAATAACTCC
    GTAACGCAAG CGGATTATAC AAAGCTACCG AAGACTGCGC CACATGGAGT AGTTCCTCCG
    CCGAATGTGA GCAATGGCCA GGGCCAGATG AATGGCAGCG GAACGCCGAG CAGCAATGGC
    AGTGGACCCT TCAAGCCGGT GCCACCACCC AAACCCAAAA ACTATAGGCC ACCGGTGCAA
    AGTGGTGGTA GCAGTGGCAG CGGAGGCACA ACTCCTTGGG AGAATGGGGA CTCTGGTTCG
    CCCCGTTCTC CGAATGGCTT CTATTATCCA CCAACGCCTT CGCATCATCA TTACGGCCAG
    CAGGCGACCC CGGGTTCACC CAGCAATGGC CACATGCAGC CACCACCACC GCAGCAGCAG
    CAGCCCACCT ACGGCGGAAG TAATGGCAAC TATGGCCAGG CACCACCACC ACAATCCTAT
    CCGCAGGCCA ATGGCTACAA TGGGAATGGG CATCACTACA ACGGGGGCAG CGGCACAGGA
    CCCTATATTG CACCACATCG CGGCATGCCA CCGCCAATAG GAAACCTGCC GCCTCACACG
    CCCGAACGTC ATGCTCTTGA CTTGGCCGGA AGTCGGGAGC AGCGCGGTTC GGCCTTCGAA
    CTGTATCGTA AACCGCAAAT CGGAGCTACC GCCGGGCACC ATCACAACAT GAGCGAAATG
    GAGCCGTACG ATGAGCGCTA CGATGATTAC TATAACATGC CACCCCCTGC CGCCCATCCG
    TCCCAAGGGC ATCATATGCA GCGATCCCGA TCAGCTCCTC GTTATCCACA CGAGCGACCT
    CCTCACGCCC AAGATCCGAA CTACTACGGC CACTATGGAA CGTCCAGGGG ACATAGTCAG
    CCGCGCCAGC AATATTCGCA GCATCACCAA CAGCAGTATT ACGATGATCA TGGGTTGGAG
    ATGGGTCCAC CGCCATTGCC GCCACATAAG AAGAAGAAAT CGGTGCTAAA GAGCCCACTG
    GTGGCATTAA AGAATGCACT GCTTAAGAGT ACGAGGCCTC TAAGGAGAAT GAACAGCATG
    GTGGAACCGG AGAGGAAACC GAAGGGACTT CGACGCCAGC AATCGATGCT GGAGAGGGGT
    GTGCAAAGAC CCTACTACCC GGATGAGTAT CCAACGTATC CAGCTGGCTT TGAGGAGCGT
    GCCCATGGTG GACAAGGCAT GATGCAACCA CATTCACAAG ATGCCTACTA TCAAAGGGGT
    GGTCACTACG CGCCGCAACA GCAGGAGATG ATGAACAGCA CCTATCAGAA TCTGGAGGGT
    GAGGATATCT ATGGGAATAT CGGCAACACA GTTCCCAGAA TGCCGCATCA CCAGGACAAT
    GGTTACGGAT ATGACCAATA TGATCTCTAT GCGAATCGAG CGTGCATCGA TCTCGAAAGA
    AGGCAAGCAG AGGCAGCTGC AGCTTCTGGC GGACGAAATG GAAGGAGAAT TGTAAGGCGG
    CACAGCACCA CTACTGCAGA TCGTGGAGGA AATCCCGGAC CGCCAAGGAG GCCTATGATC
    AGTCCTGGTT ATGAACAAGA TCCGCAAGAG ATCTACCAGA CCCGCAACGG AGCCTACATG
    CTGCCCGATC AGCGAAGGGC GCCCAGTTCG GAGGTGATGA CCCGTAGACG ATTCTATCCG
    GGGGCTGCCA ATGAGCAGAC TGAGGAGGAA CCGCTCTACC AATCACGACG CGAAATGCAG
    CGCGAAATGC AGAGAAATCA TCTGTACCAA TCGAAACGAG AAATGCAGGA GAGGATTAGC
    CAGGGCAAGA GGGATATGGA GCGAGAATTT AGCCCCCAAA GCAGCAGCAG CCAGTCGGAA
    GCCTCCAATT CGGAGGCAAT ATACCAGAGT CGGCGGGAGG CCAAGGAAAG TGCTCTGAAA
    ACGAGGTCAC AACTGAGGGA TCAGATCTAT CAGACTAGAC GGGAAGCCCT GGACAGCATG
    GCTGAACCGA TCTATGTATC CAAAAGAGGT ATGGGCAGAC CGGCCCCAAT CTATGAGACC
    CGGGAAGAGA GTATCCTCCA GTCCCGAGAA AATGAAACAG ATGAAAAGAA GGAAAAGGAG
    TGCAAAACCG AGCAGATTCA GGTGGAGATT AATGCTCAAC CGGAAGAACA GATCGAAGAT
    TCGACCTTAA GTCGTTCGGA CCTGCAGAAA TCTTCGGACA CGGTGATAGA AAATCCAGCT
    AGAGCTCCTC TTGTGCAGGA CGAAGTAGAT GATGACGAAG AGGAGGAGGT TCAGGATCAG
    CGTGATCCCG CTGAGAGCTC TGCGGATCAG ACAGAGCAGC CAACAGCTAT TGAAAATAAT
    CTGCACAATG AAGGAATGGT GGAGCAATCA CAGAATGATT CGGATGACGT ATTCGAAGCA
    GCAGATAAGG TTTCTCCCCT GGCCCCTCCA CCAGCTCCTG TTCAACCGCC GACCCCGCGA
    TCCGGTAGAG CTCCCTTCCA CATTTCGAAT ATCCTTAAGC GAACAGCACC TCCGCCGAGT
    TCCCCAATCG GTGACAGCTG TACCTCCATC GAAACCCAGT ACACATCACA AGCCAGCTTG
    CCAGTGGGTC CACCCAATGC GACCAGTACG CCGTTTAGTA GCAGCATGTC ATTGCCCATC
    GCCGGACCAG TAAATAATCA ACCTTCGGCC AACGGACGCT TTCCCACTCT GCCCCGTGAA
    CCCAGCACCT CGCGCGGATT TTTTGACTCC AACGGTGGCA CTTTGGCGGA TAAGCTGTGG
    CACGTCTCAC TGCAAATCCC GCCGGGAGCC ATTCCGGCCG GAGTACGACA AGAGATCTAC
    TTCACCGTGA GCGACCCGCG AATGGGACAA GCTGTCGGCG GTCCTCCGCT GGACATGGAA
    AATGGTGAGA CAATGCTGTC ACCGTTGGTG ATGTGCGGGC CGCAGGGCCT CGAGTTCCTC
    GTACCGGTTA CGCTCAACAT ACCCCACTGC GCCGGACGAA CTGCATCACT GGGACTGGCC
    CTCAAGGCGA CCGATAGCGA GAAGAATCTG CACACCGAGT GGGACAACAT CGACCTGCCC
    AGCAATGCCG CCGCTCACAC GGTCTCCGTC AAGGTGGATC ACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246998.1
    CDS319..7506
    Translation

    Target ORF information:

    RefSeq Version NM_001260069.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAAGCCCA GCACGCGACG TCGCAGTTTC AAAACGAAAC CCCCCAAATC GAATGTATAT 
    AACCCACTCG TTCGCTATCG CCCGACAGCC TATGTCCGAA ATGGAATTAT TGGCGGCGCC
    GAGGGTCCCG GCTCGAGTGG AGTGTACCAA ATCGCGGATA GGGTCTATAC CACCAGCAAG
    ATGTTTCTAC GCGATGGCTA TATGTACCCG CGTCAACCGG GTCAGACCAT GGATCCCCGT
    TTCCCCCTCG AGTTGCACGA ATTCCCTCAG CAATTCGGCG CCACTGGCCT GCAGTTGGTT
    AGTGCACCCA AGGTTCATAT AGCCGGCGAA TATCTGGCCA GTGAACAACA GCAGCAGCAG
    CAACAACAGC AGCAGCAGCA TCAACAGGTG CAGGAGCAAC AACAATTGGG CAGAAGAAAG
    GCTGCGGCAG CCGCCGAAAT AAATTTGGCA AATCAGGTTT ACTGGCTGGG CAAGCAAAAG
    ACCAGATCCC GATCGCGTAG CAATAGTGTG GGCAATCGGA GCATAACCAG CTGCATGGGC
    GGCGGTGGAG GGGGTGGCGA AAAGGAAAGG GAACGCGGCG GGGATCGAGA TCGCCCCTAT
    ATACGCAACG TTGACGATCT TCTCCAGGCA CTGGGCAAAA GGGATGGGGG GGATAACAAC
    AGCAATCTGC TCGAAACTGC ATCGACGCAC TCCTCATCGC AGCGAAGGCG CTTCAAGAAG
    GGTGATCGAA CCACATGGGA GTACCACACG GTGGCGGTGA CTCGGGTGCC GGGCTATGGA
    TTCGGCATCG CCGTTTCCGG TGGACGTGAT AATCCGCACT TCGCCAACGG CGATCCCTCG
    ATAGCAGTTA GCGATGTGCT GAAAGGTGGC CCCGCCGAGG ATCGATTGCA AGTCAACGAT
    CGGATAATCT CGGTGAACGG CGTCTCTCTG GAGAACGTGG AATATGCTAC CGCGGTGCAG
    GTGTTACGCG ATAGTGGCAA CACGGTGCAA TTGGTGGTCA AGCGTCGCGT GCCCCTCAAT
    CCCATTAATG CTGCAGGTGC AGTGCAGCAT CAGCATTCAC ACAGTCTCAG TTCGGTTGGT
    CTAATGGCCA ATGGAAGTGG AGGAGTGGCG CCAACCCCCA TTACGAGTCT GAGTCAACCC
    AACTCGCTGA ACTCTTCCCT AGTGCAGAAT GCCAGCAGTG GTCAGCCCAT CAAGGTGACC
    CTCACCAAAG GCGGAAAGAA AGATGACTAT GGTGTGGTGC TCGGCTGCCG GCTGTTTGTT
    AAGGAGATCT CCTCTAAAGC TCGCGAGCAA CTAAATGCCA ATGGTTATAG CTTGCAAGAG
    GGTGACATCA TCACCCGCAT CCACAATACT AACTGCGGGG ATACGATGAG TCTTAAGGAG
    GCGAAAAAGA TCATTGATGG CTGCAAGGAG CGATTGAACC TAGTGGTTCT AAGGGACATT
    ACCAACCAAA CAGCTGTTAG CCAATTGAAT CTGAACAATT CAGCCAGCCA CCAGGCGTCG
    GGAAATATAT ACGCCACGCA TCAGCCTCAG GTATCCGGAT GTAGCAGCAG TAACAACAAT
    CTTGAAGATC CATATCTGCC GGGCGGAGCC AGTTACTCCT CGCAGAATCT GTACGTGCAA
    CCGCCAACTC GCACCTCCAA TGGTCCCAAT ATCAATGGCA ATGGCCTGAA CGACGAAAAG
    AGTAATCTTA CGCCGCGAGG TCGCTCCCGG GGCCCCATAA TGGATGGGGT ATCCCTGCAG
    CAACTGGATA GACCCGTAAC ACCGACTCGG GGCAGGAGTG CAGCCATCGA CGAGCCGCCG
    CGTCCACCAC CACCAAGGGG ATCGAGCGGA GGAGCAGCTC AAGAGGATTT CTACAGCTCT
    CGCAGGCAAT TGTACGAGGA GCGTCAGAGT GCCGAACCGA GATTCATTTC CTTCCAGAAG
    GAAGGAAGCG TAGGCATCCG GCTAACCGGC GGCAATGAAG CTGGTATTTT TGTGACTGCC
    GTACAACCAG GATCTCCAGC CTCGCTGCAG GGTCTAATGC CTGGCGATAA GATCCTCAAG
    GTCAACGACA TGGACATGAA CGGAGTGACG CGCGAGGAGG CTGTTCTCTT CCTGTTGAGT
    CTCCAGGATC GCATCGATCT GATTGTGCAG TACTGCAAGG AGGAATACGA CGAGGTGGTG
    ACCAACCAAC GTGGCGACTC CTTCCACATC AAGACACATT TCCACTGCGA TAATCCTTCC
    AAAGGCGAGA TGGCCTTCAA GGCGGGCGAT GTTTTCCGGG TGATCGATAC CCTGCACAAT
    GGTGTGGTCG GATCCTGGCA GGTGCTGAAG ATCGGTCGGG GTCACCAGGA GATGCAGCGC
    GGCGTAATAC CGAACAAGTC GCGGGCTGAA GAGCTGGCCA CTGCTCAGTT CAATGCCACC
    AAGAAGGAGA TGAATGCAAA TGAATCGAGA GGCAACTTCT TCCGGCGACG ACGTTCGACG
    CACCGACGCT CGAAGAGTCT GTCCCGCGAG AACTGGGACG ATGTGGTCTT CTCCGATAGC
    ATTTCCAAAT TCCCCGCCTA CGAGCGTGTC GTTCTGCGCC ATCCAGGCTT TGTCCGACCC
    GTAGTGCTCT TCGGACCCGT ATCCGATTTG GCCCGCGAGC GATTGGCCAA AGATTTCCCC
    GATAAGTTTT CGACTCCACT GCAGGATGAC GACAAGTCAG CCGCAACTAG TGGAAAGTGC
    CGCATCGTAA GGCTCTCGAA TATACGCGAT GTGATGGATC GTGGAAAGCA CGCCCTGCTG
    GACATTACGC CCAATGCAGT GGATCGTCTC AATTACGCGC AGTTCTATCC GGTGGTGATC
    TTCCTGAAGA CAGACAGCAA GCACGTGATC AAGCAGCTGC GTCACGGACT TCCCAAGGCG
    GCACACAAGA GCTCCAAGAA GCTGCTGGAG CAGTGCCAGA AACTGGAGCG CGTCTGGTCA
    CACATCTTTA GCACCCAGAT CGCGTTGAGC GACGAGGAGT CCTGGTACCG AAAGTTGCGC
    GATTCGATCG ATCTGCAGCA GAGCGGCGCC GTGTGGATGT CCGAGTCTAA GCCAGTAGAA
    TCCCTCTCCG ACGATTTCCT ATTCCCCATG ACCACATCCC GACTCTCGTA TGCATCAAGT
    CCCGAATCCG ATTTGGAACT GAGTCCCGGC CCATCCGCAT CATTGTCGCT TGGCAATTTG
    CCGCAGTTGG TTAAAGCGAG CTCAGATCCA TCGATTGCCA CTAATCAGGA TAACTTGGAT
    AGGGATAGAG ACATAATTGG TGAAGGACTA CCACCTCCAT ATACGGTACC CTACGACCAT
    GCTGTCCCCG CGAATCCCAA TCGCCGCCAG ACCATGGACT CCAGCAAGTA CAGCATCTAC
    GGCACCAATG TGCCACCACA GCAGCAGCAG CCCGGCGTTG GAGGAGATAC GGCTGCAGTG
    AGACCGCAAT CTCTGTACGG GATTAATGCT CCCGATCTGC CGCCCCGCAT CGATCGTCAG
    TCCAAGCCGG GCGATATACC ACTGAACACC TCGGGCTCAT CGTCGCGAAA TGGCACCCTG
    GGTCGAAGTG CCCAGGAGCG TCTGTTTGGC AAGGCTGTGG TGCAGGACGA TGTCCAGGCG
    GAGTACATAA CCCGAAACGC TCTGGTCGGG TCTGGAGCTG CAGAAACCCT GGATCGCCAG
    CAGCAGCAGC AGCAGCAGAC TCATGCATCC TTGGAGCGCC AAGCGCGTTT GAATGCGCAG
    CTAAAGGCCA ACGGAGTAGG CGCCGGAGGA GGTGGTGCAT CCACCTACGA CAGTGTGTCC
    AGCTACGACT CGTACAACAA TACACAAATG GCCATGCAAA ACCTGGGCCG ACTAGGTCCC
    AATGCACCCG ACGATCTCAA ATCAGTGCCA AATGCAAATG GTCGCCCCTT GCCTCCGACG
    GGTCAGTCGC ACGAGTACGG TCGCACTCCG CACGATCATC GCAGCTTCGG AGGACCCAAC
    GATCTGAACC GGCAGAGCAG TCCGGGCAGG CCGCACTACC ATGACATGAA TGCGTCCCGT
    AATATCGATC CACGCAATGG CACACCACAG CGTCCTTCGA ATCTGGGCCT GGAAAGCAGC
    CCACGCAAGC CTTTGGTGGA GACAAAAACG GATTACGGCA AATACAGTCG GAATAACTCC
    GTAACGCAAG CGGATTATAC AAAGCTACCG AAGACTGCGC CACATGGAGT AGTTCCTCCG
    CCGAATGTGA GCAATGGCCA GGGCCAGATG AATGGCAGCG GAACGCCGAG CAGCAATGGC
    AGTGGACCCT TCAAGCCGGT GCCACCACCC AAACCCAAAA ACTATAGGCC ACCGGTGCAA
    AGTGGTGGTA GCAGTGGCAG CGGAGGCACA ACTCCTTGGG AGAATGGGGA CTCTGGTTCG
    CCCCGTTCTC CGAATGGCTT CTATTATCCA CCAACGCCTT CGCATCATCA TTACGGCCAG
    CAGGCGACCC CGGGTTCACC CAGCAATGGC CACATGCAGC CACCACCACC GCAGCAGCAG
    CAGCCCACCT ACGGCGGAAG TAATGGCAAC TATGGCCAGG CACCACCACC ACAATCCTAT
    CCGCAGGCCA ATGGCTACAA TGGGAATGGG CATCACTACA ACGGGGGCAG CGGCACAGGA
    CCCTATATTG CACCACATCG CGGCATGCCA CCGCCAATAG GAAACCTGCC GCCTCACACG
    CCCGAACGTC ATGCTCTTGA CTTGGCCGGA AGTCGGGAGC AGCGCGGTTC GGCCTTCGAA
    CTGTATCGTA AACCGCAAAT CGGAGCTACC GCCGGGCACC ATCACAACAT GAGCGAAATG
    GAGCCGTACG ATGAGCGCTA CGATGATTAC TATAACATGC CACCCCCTGC CGCCCATCCG
    TCCCAAGGGC ATCATATGCA GCGATCCCGA TCAGCTCCTC GTTATCCACA CGAGCGACCT
    CCTCACGCCC AAGATCCGAA CTACTACGGC CACTATGGAA CGTCCAGGGG ACATAGTCAG
    CCGCGCCAGC AATATTCGCA GCATCACCAA CAGCAGTATT ACGATGATCA TGGGTTGGAG
    ATGGGTCCAC CGCCATTGCC GCCACATAAG AAGAAGAAAT CGGTGCTAAA GAGCCCACTG
    GTGGCATTAA AGAATGCACT GCTTAAGAGT ACGAGGCCTC TAAGGAGAAT GAACAGCATG
    GTGGAACCGG AGAGGAAACC GAAGGGACTT CGACGCCAGC AATCGATGCT GGAGAGGGGT
    GTGCAAAGAC CCTACTACCC GGATGAGTAT CCAACGTATC CAGCTGGCTT TGAGGAGCGT
    GCCCATGGTG GACAAGGCAT GATGCAACCA CATTCACAAG ATGCCTACTA TCAAAGGGGT
    GGTCACTACG CGCCGCAACA GCAGGAGATG ATGAACAGCA CCTATCAGAA TCTGGAGGGT
    GAGGATATCT ATGGGAATAT CGGCAACACA GTTCCCAGAA TGCCGCATCA CCAGGACAAT
    GGTTACGGAT ATGACCAATA TGATCTCTAT GCGAATCGAG CGTGCATCGA TCTCGAAAGA
    AGGCAAGCAG AGGCAGCTGC AGCTTCTGGC GGACGAAATG GAAGGAGAAT TGTAAGGCGG
    CACAGCACCA CTACTGCAGA TCGTGGAGGA AATCCCGGAC CGCCAAGGAG GCCTATGATC
    AGTCCTGGTT ATGAACAAGA TCCGCAAGAG ATCTACCAGA CCCGCAACGG AGCCTACATG
    CTGCCCGATC AGCGAAGGGC GCCCAGTTCG GAGGTGATGA CCCGTAGACG ATTCTATCCG
    GGGGCTGCCA ATGAGCAGAC TGAGGAGGAA CCGCTCTACC AATCACGACG CGAAATGCAG
    CGCGAAATGC AGAGAAATCA TCTGTACCAA TCGAAACGAG AAATGCAGGA GAGGATTAGC
    CAGGGCAAGA GGGATATGGA GCGAGAATTT AGCCCCCAAA GCAGCAGCAG CCAGTCGGAA
    GCCTCCAATT CGGAGGCAAT ATACCAGAGT CGGCGGGAGG CCAAGGAAAG TGCTCTGAAA
    ACGAGGTCAC AACTGAGGGA TCAGATCTAT CAGACTAGAC GGGAAGCCCT GGACAGCATG
    GCTGAACCGA TCTATGTATC CAAAAGAGGT ATGGGCAGAC CGGCCCCAAT CTATGAGACC
    CGGGAAGAGA GTATCCTCCA GTCCCGAGAA AATGAAACAG ATGAAAAGAA GGAAAAGGAG
    TGCAAAACCG AGCAGATTCA GGTGGAGATT AATGCTCAAC CGGAAGAACA GATCGAAGAT
    TCGACCTTAA GTCGTTCGGA CCTGCAGAAA TCTTCGGACA CGGTGATAGA AAATCCAGCT
    AGAGCTCCTC TTGTGCAGGA CGAAGTAGAT GATGACGAAG AGGAGGAGGT TCAGGATCAG
    CGTGATCCCG CTGAGAGCTC TGCGGATCAG ACAGAGCAGC CAACAGCTAT TGAAAATAAT
    CTGCACAATG AAGGAATGGT GGAGCAATCA CAGAATGATT CGGATGACGT ATTCGAAGCA
    GCAGATAAGG TTTCTCCCCT GGCCCCTCCA CCAGCTCCTG TTCAACCGCC GACCCCGCGA
    TCCGGTAGAG CTCCCTTCCA CATTTCGAAT ATCCTTAAGC GAACAGCACC TCCGCCGAGT
    TCCCCAATCG GTGACAGCTG TACCTCCATC GAAACCCAGT ACACATCACA AGCCAGCTTG
    CCAGTGGGTC CACCCAATGC GACCAGTACG CCGTTTAGTA GCAGCATGTC ATTGCCCATC
    GCCGGACCAG TAAATAATCA ACCTTCGGCC AACGGACGCT TTCCCACTCT GCCCCGTGAA
    CCCAGCACCT CGCGCGGATT TTTTGACTCC AACGGTGGCA CTTTGGCGGA TAAGCTGTGG
    CACGTCTCAC TGCAAATCCC GCCGGGAGCC ATTCCGGCCG GAGTACGACA AGAGATCTAC
    TTCACCGTGA GCGACCCGCG AATGGGACAA GCTGTCGGCG GTCCTCCGCT GGACATGGAA
    AATGGTGAGA CAATGCTGTC ACCGTTGGTG ATGTGCGGGC CGCAGGGCCT CGAGTTCCTC
    GTACCGGTTA CGCTCAACAT ACCCCACTGC GCCGGACGAA CTGCATCACT GGGACTGGCC
    CTCAAGGCGA CCGATAGCGA GAAGAATCTG CACACCGAGT GGGACAACAT CGACCTGCCC
    AGCAATGCCG CCGCTCACAC GGTCTCCGTC AAGGTGGATC ACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07910
    Clone ID Related Accession (Same CDS sequence) NM_001260071.2
    Accession Version NM_001260071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product polychaetoid, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260071.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGATC CACCGCCACC GCCGCCACCC AAGCCGCAAT TGGGTTCCAA AGCGGATTCT 
    GTATCGCATT TGGCCAGCAA ATATCCCCAA TCCGAGTACA ATCTCATACA GAAAATCGAC
    TCGAACAGCA CACTCACTGC TCCGGCGCAA TATCAACCGC AGAATTTCTA CGCTAATACT
    GGCACCATAT CCTCTACGAA TGGCTACGGA TCGCTGTTAT GTCACGCCAG TTCGACGACC
    AGTCCGCTGG CGGTCCGGAA GAGGGACAAA CTGCTTCATC GCTTCAGCGA TGCCGCCACC
    TTGGGCCGGA AGCTCAAGAA GAAGAAGAAC ACGAATCGCA CCTGCAGATC CATGACGGAG
    GCCATTGAGA TGCTGGCCGA TCCCGTCATC GAAGATGAAT TCTTTGGTGA TCGAACCACA
    TGGGAGTACC ACACGGTGGC GGTGACTCGG GTGCCGGGCT ATGGATTCGG CATCGCCGTT
    TCCGGTGGAC GTGATAATCC GCACTTCGCC AACGGCGATC CCTCGATAGC AGTTAGCGAT
    GTGCTGAAAG GTGGCCCCGC CGAGGATCGA TTGCAAGTCA ACGATCGGAT AATCTCGGTG
    AACGGCGTCT CTCTGGAGAA CGTGGAATAT GCTACCGCGG TGCAGGTGTT ACGCGATAGT
    GGCAACACGG TGCAATTGGT GGTCAAGCGT CGCGTGCCCC TCAATCCCAT TAATGCTGCA
    GGTGCAGTGC AGCATCAGCA TTCACACAGT CTCAGTTCGG TTGGTCTAAT GGCCAATGGA
    AGTGGAGGAG TGGCGCCAAC CCCCATTACG AGTCTGAGTC AACCCAACTC GCTGAACTCT
    TCCCTAGTGC AGAATGCCAG CAGTGGTCAG CCCATCAAGG TGACCCTCAC CAAAGGCGGA
    AAGAAAGATG ACTATGGTGT GGTGCTCGGC TGCCGGCTGT TTGTTAAGGA GATCTCCTCT
    AAAGCTCGCG AGCAACTAAA TGCCAATGGT TATAGCTTGC AAGAGGGTGA CATCATCACC
    CGCATCCACA ATACTAACTG CGGGGATACG ATGAGTCTTA AGGAGGCGAA AAAGATCATT
    GATGGCTGCA AGGAGCGATT GAACCTAGTG GTTCTAAGGG ACATTACCAA CCAAACAGCT
    GTTAGCCAAT TGAATCTGAA CAATTCAGCC AGCCACCAGG CGTCGGGAAA TATATACGCC
    ACGCATCAGC CTCAGGTATC CGGATGTAGC AGCAGTAACA ACAATCTTGA AGATCCATAT
    CTGCCGGGCG GAGCCAGTTA CTCCTCGCAG AATCTGTACG TGCAACCGCC AACTCGCACC
    TCCAATGGTC CCAATATCAA TGGCAATGGC CTGAACGACG AAAAGAGTAA TCTTACGCCG
    CGAGGTCGCT CCCGGGGCCC CATAATGGAT GGGGTATCCC TGCAGCAACT GGATAGACCC
    GTAACACCGA CTCGGGGCAG GAGTGCAGCC ATCGACGAGC CGCCGCGTCC ACCACCACCA
    AGGGGATCGA GCGGAGGAGC AGCTCAAGAG GATTTCTACA GCTCTCGCAG GCAATTGTAC
    GAGGAGCGTC AGAGTGCCGA ACCGAGATTC ATTTCCTTCC AGAAGGAAGG AAGCGTAGGC
    ATCCGGCTAA CCGGCGGCAA TGAAGCTGGT ATTTTTGTGA CTGCCGTACA ACCAGGATCT
    CCAGCCTCGC TGCAGGGTCT AATGCCTGGC GATAAGATCC TCAAGGTCAA CGACATGGAC
    ATGAACGGAG TGACGCGCGA GGAGGCTGTT CTCTTCCTGT TGAGTCTCCA GGATCGCATC
    GATCTGATTG TGCAGTACTG CAAGGAGGAA TACGACGAGG TGGTGACCAA CCAACGTGGC
    GACTCCTTCC ACATCAAGAC ACATTTCCAC TGCGATAATC CTTCCAAAGG CGAGATGGCC
    TTCAAGGCGG GCGATGTTTT CCGGGTGATC GATACCCTGC ACAATGGTGT GGTCGGATCC
    TGGCAGGTGC TGAAGATCGG TCGGGGTCAC CAGGAGATGC AGCGCGGCGT AATACCGAAC
    AAGTCGCGGG CTGAAGAGCT GGCCACTGCT CAGTTCAATG CCACCAAGAA GGAGATGAAT
    GCAAATGAAT CGAGAGGCAA CTTCTTCCGG CGACGACGTT CGACGCACCG ACGCTCGAAG
    AGTCTGTCCC GCGAGAACTG GGACGATGTG GTCTTCTCCG ATAGCATTTC CAAATTCCCC
    GCCTACGAGC GTGTCGTTCT GCGCCATCCA GGCTTTGTCC GACCCGTAGT GCTCTTCGGA
    CCCGTATCCG ATTTGGCCCG CGAGCGATTG GCCAAAGATT TCCCCGATAA GTTTTCGACT
    CCACTGCAGG ATGACGACAA GTCAGCCGCA ACTAGTGGAA AGTGCCGCAT CGTAAGGCTC
    TCGAATATAC GCGATGTGAT GGATCGTGGA AAGCACGCCC TGCTGGACAT TACGCCCAAT
    GCAGTGGATC GTCTCAATTA CGCGCAGTTC TATCCGGTGG TGATCTTCCT GAAGACAGAC
    AGCAAGCACG TGATCAAGCA GCTGCGTCAC GGACTTCCCA AGGCGGCACA CAAGAGCTCC
    AAGAAGCTGC TGGAGCAGTG CCAGAAACTG GAGCGCGTCT GGTCACACAT CTTTAGCACC
    CAGATCGCGT TGAGCGACGA GGAGTCCTGG TACCGAAAGT TGCGCGATTC GATCGATCTG
    CAGCAGAGCG GCGCCGTGTG GATGTCCGAG TCTAAGTTCC CCGATTCCCC CACCGAAATG
    TTATTCCCCC CCTACATCGC CCAGCCATGC AAGTTAGCTT GTTGCCGCCC CATATCATCT
    ACCTCAGTGC CACGCTACTC GCAGGATATG AATCTGAATC TAAACCAGAA TCCAAGTAAA
    TCCTATTCAC TAATCCAGAA CCAGGCACAT ACCCCTCCGC CGCCACGCCC ACCGCCCGTG
    GGTTATGCCC ACAGCCAGAG TTTGGCCGAA AAGCCCCGAG ATCGCAGGTC TTATACGATG
    GTGCCGATCA TAAAGCCCGC TTCTTCGATG CCCAGTGGTC TGGCACCGAC CGTTGAGCCT
    GCTGCTCCCA TGATGCCACT AGACGATTGT GTTCTGGCCA TGGATGGGTC CAACTCCCAG
    TATCATAGTT CTCAGCCAAA TCTAATGAGT CAGAGTTATT TGAACTCGAA TTTAAATGGC
    GGCAGTACGA GTGCCGTGGA TCAAACGAAT CACTCAAACG CCATGAGGGG CTTTCAGCCC
    GGACCATCGG TAGTCAGCCG CTATGGATTG CGGTCCAATG CCCAGGTGGA ACGTAGGACC
    ACTCCCTACT ACTACCACGA GCTGCTGCTG CAGAAGAATC TCTTCGATTC CAGTCTGAAT
    CTTCTGCCCC AGCAGCAGCA GCAGCATCAG CCGGATCTGG CCAAGAGCAA GAACAGCAAT
    GTGCAGCTGG AGCAGCAACA ACAGCAGCAG CAGCAGCATC AGAAGCATCT TGCAGATGCA
    TCGAATGCAT CCAAAAGGGC ACCGATGGCC AAGCCACAGC AGCGTTTGAT TAGCAATGGT
    CAGGGCAGGA GCAACTACTC CTCCTCGGGG TCACTGAGCA ATCTTCTGGG CAATGGCCAT
    GGGCTGAGCA ATGGTCACGA GAGCAGCGAT GGCACTTTGG TGGATGAGGG CAGCTTTACA
    CTGGCCTTCA ATAATCTCAC CAATCAAATA GCCAGTCTCA ATAATTCAAG TAGTCTAAAC
    AATAATAATT GTAGCACCAG CAGGAGCATC AACAACAACA GCAACAGCCA TAATATTAAT
    GCCAACAACA ACAATAGCCA TGTTAATAAT GCTGATTCCA ACAACAATGT TGGCCAAAGT
    AACGAATGCA TTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247000.1
    CDS274..4131
    Translation

    Target ORF information:

    RefSeq Version NM_001260071.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster polychaetoid (pyd), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGATC CACCGCCACC GCCGCCACCC AAGCCGCAAT TGGGTTCCAA AGCGGATTCT 
    GTATCGCATT TGGCCAGCAA ATATCCCCAA TCCGAGTACA ATCTCATACA GAAAATCGAC
    TCGAACAGCA CACTCACTGC TCCGGCGCAA TATCAACCGC AGAATTTCTA CGCTAATACT
    GGCACCATAT CCTCTACGAA TGGCTACGGA TCGCTGTTAT GTCACGCCAG TTCGACGACC
    AGTCCGCTGG CGGTCCGGAA GAGGGACAAA CTGCTTCATC GCTTCAGCGA TGCCGCCACC
    TTGGGCCGGA AGCTCAAGAA GAAGAAGAAC ACGAATCGCA CCTGCAGATC CATGACGGAG
    GCCATTGAGA TGCTGGCCGA TCCCGTCATC GAAGATGAAT TCTTTGGTGA TCGAACCACA
    TGGGAGTACC ACACGGTGGC GGTGACTCGG GTGCCGGGCT ATGGATTCGG CATCGCCGTT
    TCCGGTGGAC GTGATAATCC GCACTTCGCC AACGGCGATC CCTCGATAGC AGTTAGCGAT
    GTGCTGAAAG GTGGCCCCGC CGAGGATCGA TTGCAAGTCA ACGATCGGAT AATCTCGGTG
    AACGGCGTCT CTCTGGAGAA CGTGGAATAT GCTACCGCGG TGCAGGTGTT ACGCGATAGT
    GGCAACACGG TGCAATTGGT GGTCAAGCGT CGCGTGCCCC TCAATCCCAT TAATGCTGCA
    GGTGCAGTGC AGCATCAGCA TTCACACAGT CTCAGTTCGG TTGGTCTAAT GGCCAATGGA
    AGTGGAGGAG TGGCGCCAAC CCCCATTACG AGTCTGAGTC AACCCAACTC GCTGAACTCT
    TCCCTAGTGC AGAATGCCAG CAGTGGTCAG CCCATCAAGG TGACCCTCAC CAAAGGCGGA
    AAGAAAGATG ACTATGGTGT GGTGCTCGGC TGCCGGCTGT TTGTTAAGGA GATCTCCTCT
    AAAGCTCGCG AGCAACTAAA TGCCAATGGT TATAGCTTGC AAGAGGGTGA CATCATCACC
    CGCATCCACA ATACTAACTG CGGGGATACG ATGAGTCTTA AGGAGGCGAA AAAGATCATT
    GATGGCTGCA AGGAGCGATT GAACCTAGTG GTTCTAAGGG ACATTACCAA CCAAACAGCT
    GTTAGCCAAT TGAATCTGAA CAATTCAGCC AGCCACCAGG CGTCGGGAAA TATATACGCC
    ACGCATCAGC CTCAGGTATC CGGATGTAGC AGCAGTAACA ACAATCTTGA AGATCCATAT
    CTGCCGGGCG GAGCCAGTTA CTCCTCGCAG AATCTGTACG TGCAACCGCC AACTCGCACC
    TCCAATGGTC CCAATATCAA TGGCAATGGC CTGAACGACG AAAAGAGTAA TCTTACGCCG
    CGAGGTCGCT CCCGGGGCCC CATAATGGAT GGGGTATCCC TGCAGCAACT GGATAGACCC
    GTAACACCGA CTCGGGGCAG GAGTGCAGCC ATCGACGAGC CGCCGCGTCC ACCACCACCA
    AGGGGATCGA GCGGAGGAGC AGCTCAAGAG GATTTCTACA GCTCTCGCAG GCAATTGTAC
    GAGGAGCGTC AGAGTGCCGA ACCGAGATTC ATTTCCTTCC AGAAGGAAGG AAGCGTAGGC
    ATCCGGCTAA CCGGCGGCAA TGAAGCTGGT ATTTTTGTGA CTGCCGTACA ACCAGGATCT
    CCAGCCTCGC TGCAGGGTCT AATGCCTGGC GATAAGATCC TCAAGGTCAA CGACATGGAC
    ATGAACGGAG TGACGCGCGA GGAGGCTGTT CTCTTCCTGT TGAGTCTCCA GGATCGCATC
    GATCTGATTG TGCAGTACTG CAAGGAGGAA TACGACGAGG TGGTGACCAA CCAACGTGGC
    GACTCCTTCC ACATCAAGAC ACATTTCCAC TGCGATAATC CTTCCAAAGG CGAGATGGCC
    TTCAAGGCGG GCGATGTTTT CCGGGTGATC GATACCCTGC ACAATGGTGT GGTCGGATCC
    TGGCAGGTGC TGAAGATCGG TCGGGGTCAC CAGGAGATGC AGCGCGGCGT AATACCGAAC
    AAGTCGCGGG CTGAAGAGCT GGCCACTGCT CAGTTCAATG CCACCAAGAA GGAGATGAAT
    GCAAATGAAT CGAGAGGCAA CTTCTTCCGG CGACGACGTT CGACGCACCG ACGCTCGAAG
    AGTCTGTCCC GCGAGAACTG GGACGATGTG GTCTTCTCCG ATAGCATTTC CAAATTCCCC
    GCCTACGAGC GTGTCGTTCT GCGCCATCCA GGCTTTGTCC GACCCGTAGT GCTCTTCGGA
    CCCGTATCCG ATTTGGCCCG CGAGCGATTG GCCAAAGATT TCCCCGATAA GTTTTCGACT
    CCACTGCAGG ATGACGACAA GTCAGCCGCA ACTAGTGGAA AGTGCCGCAT CGTAAGGCTC
    TCGAATATAC GCGATGTGAT GGATCGTGGA AAGCACGCCC TGCTGGACAT TACGCCCAAT
    GCAGTGGATC GTCTCAATTA CGCGCAGTTC TATCCGGTGG TGATCTTCCT GAAGACAGAC
    AGCAAGCACG TGATCAAGCA GCTGCGTCAC GGACTTCCCA AGGCGGCACA CAAGAGCTCC
    AAGAAGCTGC TGGAGCAGTG CCAGAAACTG GAGCGCGTCT GGTCACACAT CTTTAGCACC
    CAGATCGCGT TGAGCGACGA GGAGTCCTGG TACCGAAAGT TGCGCGATTC GATCGATCTG
    CAGCAGAGCG GCGCCGTGTG GATGTCCGAG TCTAAGTTCC CCGATTCCCC CACCGAAATG
    TTATTCCCCC CCTACATCGC CCAGCCATGC AAGTTAGCTT GTTGCCGCCC CATATCATCT
    ACCTCAGTGC CACGCTACTC GCAGGATATG AATCTGAATC TAAACCAGAA TCCAAGTAAA
    TCCTATTCAC TAATCCAGAA CCAGGCACAT ACCCCTCCGC CGCCACGCCC ACCGCCCGTG
    GGTTATGCCC ACAGCCAGAG TTTGGCCGAA AAGCCCCGAG ATCGCAGGTC TTATACGATG
    GTGCCGATCA TAAAGCCCGC TTCTTCGATG CCCAGTGGTC TGGCACCGAC CGTTGAGCCT
    GCTGCTCCCA TGATGCCACT AGACGATTGT GTTCTGGCCA TGGATGGGTC CAACTCCCAG
    TATCATAGTT CTCAGCCAAA TCTAATGAGT CAGAGTTATT TGAACTCGAA TTTAAATGGC
    GGCAGTACGA GTGCCGTGGA TCAAACGAAT CACTCAAACG CCATGAGGGG CTTTCAGCCC
    GGACCATCGG TAGTCAGCCG CTATGGATTG CGGTCCAATG CCCAGGTGGA ACGTAGGACC
    ACTCCCTACT ACTACCACGA GCTGCTGCTG CAGAAGAATC TCTTCGATTC CAGTCTGAAT
    CTTCTGCCCC AGCAGCAGCA GCAGCATCAG CCGGATCTGG CCAAGAGCAA GAACAGCAAT
    GTGCAGCTGG AGCAGCAACA ACAGCAGCAG CAGCAGCATC AGAAGCATCT TGCAGATGCA
    TCGAATGCAT CCAAAAGGGC ACCGATGGCC AAGCCACAGC AGCGTTTGAT TAGCAATGGT
    CAGGGCAGGA GCAACTACTC CTCCTCGGGG TCACTGAGCA ATCTTCTGGG CAATGGCCAT
    GGGCTGAGCA ATGGTCACGA GAGCAGCGAT GGCACTTTGG TGGATGAGGG CAGCTTTACA
    CTGGCCTTCA ATAATCTCAC CAATCAAATA GCCAGTCTCA ATAATTCAAG TAGTCTAAAC
    AATAATAATT GTAGCACCAG CAGGAGCATC AACAACAACA GCAACAGCCA TAATATTAAT
    GCCAACAACA ACAATAGCCA TGTTAATAAT GCTGATTCCA ACAACAATGT TGGCCAAAGT
    AACGAATGCA TTGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.