alpha-Est10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-Est10
Entrez Gene ID	40896
Full Name	alpha-Esterase-10
Synonyms	CG1131,DmalphaE10,Dmel\CG1131,Esterase-10,K,aE10,alpha-Ests,alphaE10,anon-WO0172774.144
General protein information	Preferred Names alpha-Esterase-10 Names CG1131 gene product from transcript CG1131-RC CG1131-PB CG1131-PC CG1131-PD Esterase-10 alpha-Est10-PB alpha-Est10-PC alpha-Est10-PD fragment K
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84D8-84D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_079533.4	NP_524257.3	alpha-Esterase-10, isoform B
NM_001260033.2	NP_001246962.1	alpha-Esterase-10, isoform C
NM_001260034.1	NP_001246963.1	alpha-Esterase-10, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	alpha-Est5	NP_001246965.1
Drosophila melanogaster (fruit fly)	alpha-Est1	NP_524269.3
Drosophila melanogaster (fruit fly)	alpha-Est2	NP_001262345.1
Drosophila melanogaster (fruit fly)	alpha-Est6	NP_524262.1
Drosophila melanogaster (fruit fly)	alpha-Est9	NP_731165.2
Drosophila melanogaster (fruit fly)	CG6018	NP_611678.1
Drosophila melanogaster (fruit fly)	alpha-Est8	NP_524259.2
Drosophila melanogaster (fruit fly)	alpha-Est4	NP_524266.1
Drosophila melanogaster (fruit fly)	alpha-Est3	NP_001163540.1
Drosophila melanogaster (fruit fly)	alpha-Est7	NP_524261.1
Drosophila melanogaster (fruit fly)	alpha-Est10	NP_001246963.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alpha-Est10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Est10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04202	NM_001260033.2 Latest version!	Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa04203	NM_001260034.1 Latest version!	Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07940	NM_079533.4 Latest version!	Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-Est10 ( NM_001260033.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246962.1 Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04202
Clone ID Related Accession (Same CDS sequence)	NM_001260033.2
Accession Version	NM_001260033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Esterase-10, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260033.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAAGGT ATTGGGTATC TCTACTAAGG CATTTTAGCC TCATTCCGAA CAAAAGTGTA 
CCGAAAGTCT TCACCAATGG GGATTTCATT CAGCAGGTAA TAAGCTTCAA GTACGAGCAG 
CGCAAATTGT CAACAGCAAT ATATTCCGTC ATAAAGACGA AATCAGGTCC TGTGCGGGGA 
GTGAAGAGAA ACACAATCTG GGGAGGAAGC TACTTCAGTT TCGAGAAGAT ACCCTTCGCA 
AAGCCTCCGG TGGGAGATCT GCGCTTCAAG GCCCCGGAAG CAGTGGAGCC ATGGGATCAG 
GAATTGGATT GCACTTCGCC GGCAGACAAG CCCCTTCAGA CACACATGTT TTTCAGAAAA 
TACGCGGGCT CAGAGGACTG CCTCTACTTA AATGTGTATG TCAAAGATCT GCAGCCGGAT 
AAACTGCGTC CCGTGATGGT TTGGATCTAC GGAGGAGGCT ATCAAGTTGG CGAAGCTTCT 
CGGGATATGT ACAGTCCCGA CTTTTTCATG TCGAAGGATG TGGTCATAGT CACCGTTGCT 
TATCGACTGG GTGCCTTGGG CTTCCTCAGC CTGGATGATC CCCAACTAAA CGTTCCCGGA 
AATGCAGGTC TCAAGGATCA AATCATGGCC CTGCGATGGG TGCAACAAAA CATCGAAGCA 
TTCGGCGGTG ATTCCAACAA TATTACACTC TTTGGCGAAA GTGCCGGCGG AGCCTCGACC 
CACTTCCTTG CACTAAGTCC CCAAACTGAA GGTCTTATCC ACAAAGCTAT CGTTATGTCG 
GGCAGTGTTT TGTGCCCCTG GACGCAACCA CCGAGAAATA ATTGGGCTTA TAGGCTGGCC 
CAAAAATTGG GATACACCGG TGACAATAAG GACAAGGCGA TCTTTGAGTT TCTGCGATCA 
ATGAGTGGCG GGGAGATTGT CAAGGCCACC GCAACAGTTC TCAGCAACGA TGAAAAGCAT 
CATCGGATCC TTTTCGCCTT CGGACCTGTC GTAGAACCAT ATACTACCGA GCACACTGTG 
GTCGCTAAAC AACCGCATGA ACTGATGCAG AATAGCTGGA GTCACAGGAT ACCCATGATG 
TTTGGAGGCA CGAGCTTCGA GGGATTGCTA TTCTATCCAG AGGTTTCAAG GCGGCCAGCA 
ACCCTCGATG AGGTGGGTAA CTGCAAGAAT CTGCTACCGA GCGATCTCGG TCTTAACCTA 
GATCCCAAAC TGCGTGAGAA CTACGGCTTG CAACTGAAGA AGGCGTATTT CGGCGACGAA 
CCCTGTAACC AGGCAAACAT GATGAAGTTT CTCGAGCTAT GCTCATATCG AGAGTTCTGG 
CACCCTATAT ACAGGGCAGC TTTGAACCGT GTCCGGCAAT CCAGCGCACC CACGTATCTG 
TATCGATTCG ATCACGATTC CAAACTGTGC AACGCCATTA GGATTGTACT TTGCGGCCAT 
CAGATGCGAG GTGTTTGTCA TGGTGACGAT CTGTGCTATA TTTTCCACAG CATGTTGTCG 
CATCAATCCG CTCCCGATTC TCCGGAACAC AAGGTTATAA CCGGAATGGT CGACGTTTGG 
ACGAGTTTCG CAGCCCACGG AGATCCCAAC TGCGAAAGTA TAAAATCACT CAAGTTTGCA 
CCCATCGAAA ACGTAACCAA CTTTAAGTGT CTCAATATTG GGGATCAGTT TGAAGTCATG 
GCGCTTCCAG AATTGCAGAA AATCGAACCT GTGTGGAATA GTTTCTACGC CCCAAACAAA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246962.1
CDS	23..1768
Translation	MRRYWVSLLRHFSLIPNKSVPKVFTNGDFIQQVISFKYEQRKLSTAIYSVIKTKSGPVRGVKRNTIWGGSYFSFEKIPFAKPPVGDLRFKAPEAVEPWDQELDCTSPADKPLQTHMFFRKYAGSEDCLYLNVYVKDLQPDKLRPVMVWIYGGGYQVGEASRDMYSPDFFMSKDVVIVTVAYRLGALGFLSLDDPQLNVPGNAGLKDQIMALRWVQQNIEAFGGDSNNITLFGESAGGASTHFLALSPQTEGLIHKAIVMSGSVLCPWTQPPRNNWAYRLAQKLGYTGDNKDKAIFEFLRSMSGGEIVKATATVLSNDEKHHRILFAFGPVVEPYTTEHTVVAKQPHELMQNSWSHRIPMMFGGTSFEGLLFYPEVSRRPATLDEVGNCKNLLPSDLGLNLDPKLRENYGLQLKKAYFGDEPCNQANMMKFLELCSYREFWHPIYRAALNRVRQSSAPTYLYRFDHDSKLCNAIRIVLCGHQMRGVCHGDDLCYIFHSMLSHQSAPDSPEHKVITGMVDVWTSFAAHGDPNCESIKSLKFAPIENVTNFKCLNIGDQFEVMALPELQKIEPVWNSFYAPNKL

Target ORF information:

RefSeq Version	NM_001260033.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260033.2

ORF Insert Sequence:

ATGAGAAGGT ATTGGGTATC TCTACTAAGG CATTTTAGCC TCATTCCGAA CAAAAGTGTA 
CCGAAAGTCT TCACCAATGG GGATTTCATT CAGCAGGTAA TAAGCTTCAA GTACGAGCAG 
CGCAAATTGT CAACAGCAAT ATATTCCGTC ATAAAGACGA AATCAGGTCC TGTGCGGGGA 
GTGAAGAGAA ACACAATCTG GGGAGGAAGC TACTTCAGTT TCGAGAAGAT ACCCTTCGCA 
AAGCCTCCGG TGGGAGATCT GCGCTTCAAG GCCCCGGAAG CAGTGGAGCC ATGGGATCAG 
GAATTGGATT GCACTTCGCC GGCAGACAAG CCCCTTCAGA CACACATGTT TTTCAGAAAA 
TACGCGGGCT CAGAGGACTG CCTCTACTTA AATGTGTATG TCAAAGATCT GCAGCCGGAT 
AAACTGCGTC CCGTGATGGT TTGGATCTAC GGAGGAGGCT ATCAAGTTGG CGAAGCTTCT 
CGGGATATGT ACAGTCCCGA CTTTTTCATG TCGAAGGATG TGGTCATAGT CACCGTTGCT 
TATCGACTGG GTGCCTTGGG CTTCCTCAGC CTGGATGATC CCCAACTAAA CGTTCCCGGA 
AATGCAGGTC TCAAGGATCA AATCATGGCC CTGCGATGGG TGCAACAAAA CATCGAAGCA 
TTCGGCGGTG ATTCCAACAA TATTACACTC TTTGGCGAAA GTGCCGGCGG AGCCTCGACC 
CACTTCCTTG CACTAAGTCC CCAAACTGAA GGTCTTATCC ACAAAGCTAT CGTTATGTCG 
GGCAGTGTTT TGTGCCCCTG GACGCAACCA CCGAGAAATA ATTGGGCTTA TAGGCTGGCC 
CAAAAATTGG GATACACCGG TGACAATAAG GACAAGGCGA TCTTTGAGTT TCTGCGATCA 
ATGAGTGGCG GGGAGATTGT CAAGGCCACC GCAACAGTTC TCAGCAACGA TGAAAAGCAT 
CATCGGATCC TTTTCGCCTT CGGACCTGTC GTAGAACCAT ATACTACCGA GCACACTGTG 
GTCGCTAAAC AACCGCATGA ACTGATGCAG AATAGCTGGA GTCACAGGAT ACCCATGATG 
TTTGGAGGCA CGAGCTTCGA GGGATTGCTA TTCTATCCAG AGGTTTCAAG GCGGCCAGCA 
ACCCTCGATG AGGTGGGTAA CTGCAAGAAT CTGCTACCGA GCGATCTCGG TCTTAACCTA 
GATCCCAAAC TGCGTGAGAA CTACGGCTTG CAACTGAAGA AGGCGTATTT CGGCGACGAA 
CCCTGTAACC AGGCAAACAT GATGAAGTTT CTCGAGCTAT GCTCATATCG AGAGTTCTGG 
CACCCTATAT ACAGGGCAGC TTTGAACCGT GTCCGGCAAT CCAGCGCACC CACGTATCTG 
TATCGATTCG ATCACGATTC CAAACTGTGC AACGCCATTA GGATTGTACT TTGCGGCCAT 
CAGATGCGAG GTGTTTGTCA TGGTGACGAT CTGTGCTATA TTTTCCACAG CATGTTGTCG 
CATCAATCCG CTCCCGATTC TCCGGAACAC AAGGTTATAA CCGGAATGGT CGACGTTTGG 
ACGAGTTTCG CAGCCCACGG AGATCCCAAC TGCGAAAGTA TAAAATCACT CAAGTTTGCA 
CCCATCGAAA ACGTAACCAA CTTTAAGTGT CTCAATATTG GGGATCAGTT TGAAGTCATG 
GCGCTTCCAG AATTGCAGAA AATCGAACCT GTGTGGAATA GTTTCTACGC CCCAAACAAA 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Est10 ( NM_001260034.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246963.1 Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04203
Clone ID Related Accession (Same CDS sequence)	NM_001260034.1
Accession Version	NM_001260034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Esterase-10, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTAATAA GCTTCAAGTA CGAGCAGCGC AAATTGTCAA CAGCAATATA TTCCGTCATA 
AAGACGAAAT CAGGTCCTGT GCGGGGAGTG AAGAGAAACA CAATCTGGGG AGGAAGCTAC 
TTCAGTTTCG AGAAGATACC CTTCGCAAAG CCTCCGGTGG GAGATCTGCG CTTCAAGGCC 
CCGGAAGCAG TGGAGCCATG GGATCAGGAA TTGGATTGCA CTTCGCCGGC AGACAAGCCC 
CTTCAGACAC ACATGTTTTT CAGAAAATAC GCGGGCTCAG AGGACTGCCT CTACTTAAAT 
GTGTATGTCA AAGATCTGCA GCCGGATAAA CTGCGTCCCG TGATGGTTTG GATCTACGGA 
GGAGGCTATC AAGTTGGCGA AGCTTCTCGG GATATGTACA GTCCCGACTT TTTCATGTCG 
AAGGATGTGG TCATAGTCAC CGTTGCTTAT CGACTGGGTG CCTTGGGCTT CCTCAGCCTG 
GATGATCCCC AACTAAACGT TCCCGGAAAT GCAGGTCTCA AGGATCAAAT CATGGCCCTG 
CGATGGGTGC AACAAAACAT CGAAGCATTC GGCGGTGATT CCAACAATAT TACACTCTTT 
GGCGAAAGTG CCGGCGGAGC CTCGACCCAC TTCCTTGCAC TAAGTCCCCA AACTGAAGGT 
CTTATCCACA AAGCTATCGT TATGTCGGGC AGTGTTTTGT GCCCCTGGAC GCAACCACCG 
AGAAATAATT GGGCTTATAG GCTGGCCCAA AAATTGGGAT ACACCGGTGA CAATAAGGAC 
AAGGCGATCT TTGAGTTTCT GCGATCAATG AGTGGCGGGG AGATTGTCAA GGCCACCGCA 
ACAGTTCTCA GCAACGATGA AAAGCATCAT CGGATCCTTT TCGCCTTCGG ACCTGTCGTA 
GAACCATATA CTACCGAGCA CACTGTGGTC GCTAAACAAC CGCATGAACT GATGCAGAAT 
AGCTGGAGTC ACAGGATACC CATGATGTTT GGAGGCACGA GCTTCGAGGG ATTGCTATTC 
TATCCAGAGG TTTCAAGGCG GCCAGCAACC CTCGATGAGG TGGGTAACTG CAAGAATCTG 
CTACCGAGCG ATCTCGGTCT TAACCTAGAT CCCAAACTGC GTGAGAACTA CGGCTTGCAA 
CTGAAGAAGG CGTATTTCGG CGACGAACCC TGTAACCAGG CAAACATGAT GAAGTTTCTC 
GAGCTATGCT CATATCGAGA GTTCTGGCAC CCTATATACA GGGCAGCTTT GAACCGTGTC 
CGGCAATCCA GCGCACCCAC GTATCTGTAT CGATTCGATC ACGATTCCAA ACTGTGCAAC 
GCCATTAGGA TTGTACTTTG CGGCCATCAG ATGCGAGGTG TTTGTCATGG TGACGATCTG 
TGCTATATTT TCCACAGCAT GTTGTCGCAT CAATCCGCTC CCGATTCTCC GGAACACAAG 
GTTATAACCG GAATGGTCGA CGTTTGGACG AGTTTCGCAG CCCACGGAGA TCCCAACTGC 
GAAAGTATAA AATCACTCAA GTTTGCACCC ATCGAAAACG TAACCAACTT TAAGTGTCTC 
AATATTGGGG ATCAGTTTGA AGTCATGGCG CTTCCAGAAT TGCAGAAAAT CGAACCTGTG 
TGGAATAGTT TCTACGCCCC AAACAAACTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246963.1
CDS	62..1714
Translation	MVISFKYEQRKLSTAIYSVIKTKSGPVRGVKRNTIWGGSYFSFEKIPFAKPPVGDLRFKAPEAVEPWDQELDCTSPADKPLQTHMFFRKYAGSEDCLYLNVYVKDLQPDKLRPVMVWIYGGGYQVGEASRDMYSPDFFMSKDVVIVTVAYRLGALGFLSLDDPQLNVPGNAGLKDQIMALRWVQQNIEAFGGDSNNITLFGESAGGASTHFLALSPQTEGLIHKAIVMSGSVLCPWTQPPRNNWAYRLAQKLGYTGDNKDKAIFEFLRSMSGGEIVKATATVLSNDEKHHRILFAFGPVVEPYTTEHTVVAKQPHELMQNSWSHRIPMMFGGTSFEGLLFYPEVSRRPATLDEVGNCKNLLPSDLGLNLDPKLRENYGLQLKKAYFGDEPCNQANMMKFLELCSYREFWHPIYRAALNRVRQSSAPTYLYRFDHDSKLCNAIRIVLCGHQMRGVCHGDDLCYIFHSMLSHQSAPDSPEHKVITGMVDVWTSFAAHGDPNCESIKSLKFAPIENVTNFKCLNIGDQFEVMALPELQKIEPVWNSFYAPNKL

Target ORF information:

RefSeq Version	NM_001260034.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260034.1

ORF Insert Sequence:

ATGGTAATAA GCTTCAAGTA CGAGCAGCGC AAATTGTCAA CAGCAATATA TTCCGTCATA 
AAGACGAAAT CAGGTCCTGT GCGGGGAGTG AAGAGAAACA CAATCTGGGG AGGAAGCTAC 
TTCAGTTTCG AGAAGATACC CTTCGCAAAG CCTCCGGTGG GAGATCTGCG CTTCAAGGCC 
CCGGAAGCAG TGGAGCCATG GGATCAGGAA TTGGATTGCA CTTCGCCGGC AGACAAGCCC 
CTTCAGACAC ACATGTTTTT CAGAAAATAC GCGGGCTCAG AGGACTGCCT CTACTTAAAT 
GTGTATGTCA AAGATCTGCA GCCGGATAAA CTGCGTCCCG TGATGGTTTG GATCTACGGA 
GGAGGCTATC AAGTTGGCGA AGCTTCTCGG GATATGTACA GTCCCGACTT TTTCATGTCG 
AAGGATGTGG TCATAGTCAC CGTTGCTTAT CGACTGGGTG CCTTGGGCTT CCTCAGCCTG 
GATGATCCCC AACTAAACGT TCCCGGAAAT GCAGGTCTCA AGGATCAAAT CATGGCCCTG 
CGATGGGTGC AACAAAACAT CGAAGCATTC GGCGGTGATT CCAACAATAT TACACTCTTT 
GGCGAAAGTG CCGGCGGAGC CTCGACCCAC TTCCTTGCAC TAAGTCCCCA AACTGAAGGT 
CTTATCCACA AAGCTATCGT TATGTCGGGC AGTGTTTTGT GCCCCTGGAC GCAACCACCG 
AGAAATAATT GGGCTTATAG GCTGGCCCAA AAATTGGGAT ACACCGGTGA CAATAAGGAC 
AAGGCGATCT TTGAGTTTCT GCGATCAATG AGTGGCGGGG AGATTGTCAA GGCCACCGCA 
ACAGTTCTCA GCAACGATGA AAAGCATCAT CGGATCCTTT TCGCCTTCGG ACCTGTCGTA 
GAACCATATA CTACCGAGCA CACTGTGGTC GCTAAACAAC CGCATGAACT GATGCAGAAT 
AGCTGGAGTC ACAGGATACC CATGATGTTT GGAGGCACGA GCTTCGAGGG ATTGCTATTC 
TATCCAGAGG TTTCAAGGCG GCCAGCAACC CTCGATGAGG TGGGTAACTG CAAGAATCTG 
CTACCGAGCG ATCTCGGTCT TAACCTAGAT CCCAAACTGC GTGAGAACTA CGGCTTGCAA 
CTGAAGAAGG CGTATTTCGG CGACGAACCC TGTAACCAGG CAAACATGAT GAAGTTTCTC 
GAGCTATGCT CATATCGAGA GTTCTGGCAC CCTATATACA GGGCAGCTTT GAACCGTGTC 
CGGCAATCCA GCGCACCCAC GTATCTGTAT CGATTCGATC ACGATTCCAA ACTGTGCAAC 
GCCATTAGGA TTGTACTTTG CGGCCATCAG ATGCGAGGTG TTTGTCATGG TGACGATCTG 
TGCTATATTT TCCACAGCAT GTTGTCGCAT CAATCCGCTC CCGATTCTCC GGAACACAAG 
GTTATAACCG GAATGGTCGA CGTTTGGACG AGTTTCGCAG CCCACGGAGA TCCCAACTGC 
GAAAGTATAA AATCACTCAA GTTTGCACCC ATCGAAAACG TAACCAACTT TAAGTGTCTC 
AATATTGGGG ATCAGTTTGA AGTCATGGCG CTTCCAGAAT TGCAGAAAAT CGAACCTGTG 
TGGAATAGTT TCTACGCCCC AAACAAACTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

alpha-Est10 ( NM_079533.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524257.3 Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07940
Clone ID Related Accession (Same CDS sequence)	NM_079533.4
Accession Version	NM_079533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Esterase-10, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079533.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGATTCG ATATGGCAAC ACGCTTTATG GATATACTAA AGCTGACCTT TAAGGTAATA 
AGCTTCAAGT ACGAGCAGCG CAAATTGTCA ACAGCAATAT ATTCCGTCAT AAAGACGAAA 
TCAGGTCCTG TGCGGGGAGT GAAGAGAAAC ACAATCTGGG GAGGAAGCTA CTTCAGTTTC 
GAGAAGATAC CCTTCGCAAA GCCTCCGGTG GGAGATCTGC GCTTCAAGGC CCCGGAAGCA 
GTGGAGCCAT GGGATCAGGA ATTGGATTGC ACTTCGCCGG CAGACAAGCC CCTTCAGACA 
CACATGTTTT TCAGAAAATA CGCGGGCTCA GAGGACTGCC TCTACTTAAA TGTGTATGTC 
AAAGATCTGC AGCCGGATAA ACTGCGTCCC GTGATGGTTT GGATCTACGG AGGAGGCTAT 
CAAGTTGGCG AAGCTTCTCG GGATATGTAC AGTCCCGACT TTTTCATGTC GAAGGATGTG 
GTCATAGTCA CCGTTGCTTA TCGACTGGGT GCCTTGGGCT TCCTCAGCCT GGATGATCCC 
CAACTAAACG TTCCCGGAAA TGCAGGTCTC AAGGATCAAA TCATGGCCCT GCGATGGGTG 
CAACAAAACA TCGAAGCATT CGGCGGTGAT TCCAACAATA TTACACTCTT TGGCGAAAGT 
GCCGGCGGAG CCTCGACCCA CTTCCTTGCA CTAAGTCCCC AAACTGAAGG TCTTATCCAC 
AAAGCTATCG TTATGTCGGG CAGTGTTTTG TGCCCCTGGA CGCAACCACC GAGAAATAAT 
TGGGCTTATA GGCTGGCCCA AAAATTGGGA TACACCGGTG ACAATAAGGA CAAGGCGATC 
TTTGAGTTTC TGCGATCAAT GAGTGGCGGG GAGATTGTCA AGGCCACCGC AACAGTTCTC 
AGCAACGATG AAAAGCATCA TCGGATCCTT TTCGCCTTCG GACCTGTCGT AGAACCATAT 
ACTACCGAGC ACACTGTGGT CGCTAAACAA CCGCATGAAC TGATGCAGAA TAGCTGGAGT 
CACAGGATAC CCATGATGTT TGGAGGCACG AGCTTCGAGG GATTGCTATT CTATCCAGAG 
GTTTCAAGGC GGCCAGCAAC CCTCGATGAG GTGGGTAACT GCAAGAATCT GCTACCGAGC 
GATCTCGGTC TTAACCTAGA TCCCAAACTG CGTGAGAACT ACGGCTTGCA ACTGAAGAAG 
GCGTATTTCG GCGACGAACC CTGTAACCAG GCAAACATGA TGAAGTTTCT CGAGCTATGC 
TCATATCGAG AGTTCTGGCA CCCTATATAC AGGGCAGCTT TGAACCGTGT CCGGCAATCC 
AGCGCACCCA CGTATCTGTA TCGATTCGAT CACGATTCCA AACTGTGCAA CGCCATTAGG 
ATTGTACTTT GCGGCCATCA GATGCGAGGT GTTTGTCATG GTGACGATCT GTGCTATATT 
TTCCACAGCA TGTTGTCGCA TCAATCCGCT CCCGATTCTC CGGAACACAA GGTTATAACC 
GGAATGGTCG ACGTTTGGAC GAGTTTCGCA GCCCACGGAG ATCCCAACTG CGAAAGTATA 
AAATCACTCA AGTTTGCACC CATCGAAAAC GTAACCAACT TTAAGTGTCT CAATATTGGG 
GATCAGTTTG AAGTCATGGC GCTTCCAGAA TTGCAGAAAA TCGAACCTGT GTGGAATAGT 
TTCTACGCCC CAAACAAACT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524257.3
CDS	211..1914
Translation	MGFDMATRFMDILKLTFKVISFKYEQRKLSTAIYSVIKTKSGPVRGVKRNTIWGGSYFSFEKIPFAKPPVGDLRFKAPEAVEPWDQELDCTSPADKPLQTHMFFRKYAGSEDCLYLNVYVKDLQPDKLRPVMVWIYGGGYQVGEASRDMYSPDFFMSKDVVIVTVAYRLGALGFLSLDDPQLNVPGNAGLKDQIMALRWVQQNIEAFGGDSNNITLFGESAGGASTHFLALSPQTEGLIHKAIVMSGSVLCPWTQPPRNNWAYRLAQKLGYTGDNKDKAIFEFLRSMSGGEIVKATATVLSNDEKHHRILFAFGPVVEPYTTEHTVVAKQPHELMQNSWSHRIPMMFGGTSFEGLLFYPEVSRRPATLDEVGNCKNLLPSDLGLNLDPKLRENYGLQLKKAYFGDEPCNQANMMKFLELCSYREFWHPIYRAALNRVRQSSAPTYLYRFDHDSKLCNAIRIVLCGHQMRGVCHGDDLCYIFHSMLSHQSAPDSPEHKVITGMVDVWTSFAAHGDPNCESIKSLKFAPIENVTNFKCLNIGDQFEVMALPELQKIEPVWNSFYAPNKL

Target ORF information:

RefSeq Version	NM_079533.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Esterase-10 (alpha-Est10), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079533.4

ORF Insert Sequence:

ATGGGATTCG ATATGGCAAC ACGCTTTATG GATATACTAA AGCTGACCTT TAAGGTAATA 
AGCTTCAAGT ACGAGCAGCG CAAATTGTCA ACAGCAATAT ATTCCGTCAT AAAGACGAAA 
TCAGGTCCTG TGCGGGGAGT GAAGAGAAAC ACAATCTGGG GAGGAAGCTA CTTCAGTTTC 
GAGAAGATAC CCTTCGCAAA GCCTCCGGTG GGAGATCTGC GCTTCAAGGC CCCGGAAGCA 
GTGGAGCCAT GGGATCAGGA ATTGGATTGC ACTTCGCCGG CAGACAAGCC CCTTCAGACA 
CACATGTTTT TCAGAAAATA CGCGGGCTCA GAGGACTGCC TCTACTTAAA TGTGTATGTC 
AAAGATCTGC AGCCGGATAA ACTGCGTCCC GTGATGGTTT GGATCTACGG AGGAGGCTAT 
CAAGTTGGCG AAGCTTCTCG GGATATGTAC AGTCCCGACT TTTTCATGTC GAAGGATGTG 
GTCATAGTCA CCGTTGCTTA TCGACTGGGT GCCTTGGGCT TCCTCAGCCT GGATGATCCC 
CAACTAAACG TTCCCGGAAA TGCAGGTCTC AAGGATCAAA TCATGGCCCT GCGATGGGTG 
CAACAAAACA TCGAAGCATT CGGCGGTGAT TCCAACAATA TTACACTCTT TGGCGAAAGT 
GCCGGCGGAG CCTCGACCCA CTTCCTTGCA CTAAGTCCCC AAACTGAAGG TCTTATCCAC 
AAAGCTATCG TTATGTCGGG CAGTGTTTTG TGCCCCTGGA CGCAACCACC GAGAAATAAT 
TGGGCTTATA GGCTGGCCCA AAAATTGGGA TACACCGGTG ACAATAAGGA CAAGGCGATC 
TTTGAGTTTC TGCGATCAAT GAGTGGCGGG GAGATTGTCA AGGCCACCGC AACAGTTCTC 
AGCAACGATG AAAAGCATCA TCGGATCCTT TTCGCCTTCG GACCTGTCGT AGAACCATAT 
ACTACCGAGC ACACTGTGGT CGCTAAACAA CCGCATGAAC TGATGCAGAA TAGCTGGAGT 
CACAGGATAC CCATGATGTT TGGAGGCACG AGCTTCGAGG GATTGCTATT CTATCCAGAG 
GTTTCAAGGC GGCCAGCAAC CCTCGATGAG GTGGGTAACT GCAAGAATCT GCTACCGAGC 
GATCTCGGTC TTAACCTAGA TCCCAAACTG CGTGAGAACT ACGGCTTGCA ACTGAAGAAG 
GCGTATTTCG GCGACGAACC CTGTAACCAG GCAAACATGA TGAAGTTTCT CGAGCTATGC 
TCATATCGAG AGTTCTGGCA CCCTATATAC AGGGCAGCTT TGAACCGTGT CCGGCAATCC 
AGCGCACCCA CGTATCTGTA TCGATTCGAT CACGATTCCA AACTGTGCAA CGCCATTAGG 
ATTGTACTTT GCGGCCATCA GATGCGAGGT GTTTGTCATG GTGACGATCT GTGCTATATT 
TTCCACAGCA TGTTGTCGCA TCAATCCGCT CCCGATTCTC CGGAACACAA GGTTATAACC 
GGAATGGTCG ACGTTTGGAC GAGTTTCGCA GCCCACGGAG ATCCCAACTG CGAAAGTATA 
AAATCACTCA AGTTTGCACC CATCGAAAAC GTAACCAACT TTAAGTGTCT CAATATTGGG 
GATCAGTTTG AAGTCATGGC GCTTCCAGAA TTGCAGAAAA TCGAACCTGT GTGGAATAGT 
TTCTACGCCC CAAACAAACT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alpha-Est10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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