Wdr33 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wdr33
Entrez Gene ID	40698
Full Name	CG1109 gene product from transcript CG1109-RA
Synonyms	BcDNA:LD24014,Dmel\CG1109,WDR33,Wdr33
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83B8-83B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_169091.2	NP_730982.1	WD repeat domain 33, isoform A
NM_169092.2	NP_730983.1	WD repeat domain 33, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG1109	NP_730983.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001362	XP_321783.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wdr33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wdr33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03980	NM_169091.2 Latest version!	Drosophila melanogaster WD repeat domain 33 (Wdr33), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03980	NM_169092.2 Latest version!	Drosophila melanogaster WD repeat domain 33 (Wdr33), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wdr33 ( NM_169091.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730982.1 Drosophila melanogaster WD repeat domain 33 (Wdr33), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03980
Clone ID Related Accession (Same CDS sequence)	NM_169091.2 , NM_169092.2
Accession Version	NM_169091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WD repeat domain 33, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169091.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGTAT CCCAGCCGCC ACCGCAGCTG CTCACGGCGC CCTCGGCCCT GGCCACCAAC 
TTCACATCGC TGCCCCCGCC GAACATGGGC GGCCAGCACT ACCGTCACTA CCATCCGCAC 
CATGGGTCCA ACAAGCACGG CTACAACCAG TTCAAACCCT TCATGCCCGG CGGCTTCCAG 
CGACCCTTCG GAATGTCCCA GGACGACTTC GACGGCAAGC GACTGCGAAA AAGTGTGATG 
CGGAAGACAG TTGACTACAA CGCGTCCATC ATCAAGGCAC TGGAGAACCG ATTGTACCAG 
CGGGACTATC GGGACCGTTT GGCTTTGCAG CCGGACAGCA TTTATGTGCC CCATATGCTG 
CCGCCCTCTG CCTACTTGGA CAATCCTTCG AACGCGGTGA CCACAAGGTT CGTGAAGACG 
GCTACCAATA AGATGCGATG TCCGATTTTC ACACTGGCCT GGACGCCAGA GGGCAGACGT 
TTGGTAACCG GGGCCAGTTC CGGCGAGTTT ACGCTATGGA ACGGACTAAC CTTTAATTTC 
GAGACCATTC TGCAGGCCCA CGACATTTCT GTGCGGACGA TGGTGTGGTC GCACAACGAC 
AGCTGGATGG TGACCGGCGA CCATGGCGGC TATGTTAAGT ACTGGCAGTC CAACATGAAC 
AACGTGAAGA TGTACCAGGC GCACAAGGAG GCGATTAGGG GAATAAGTTT CAGTCCCACG 
GACAGCAAGT TCGTCAGCGG CAGCGACGAC GGAACCCTGA GGATATGGGA CTTCATGCGG 
TGCCAGGAGG AGCGAGTGCT ACGAGGCCAC GGTGCTGACG TCAAGTGCGT GCACTGGCAT 
CCACAAAAGG GAATGATCGT CAGCGGAAGC AAGGACAACC AGCAACCCAT CAAGATTTGG 
GATCCCAAGA GCGGCATTGC CCTGGCTACT CTACACGCCC ACAAATCCAC CGTGATGGAT 
CTGAAGTGGA ACGACAATGG CAACTGGCTG GTAACCGCAT CCAGAGATCA TCTGCTCAAG 
CTCTTCGACA TTCGCAACCT GCGTGAGGAG GTTCAGGTCT TCCGTGGCCA CAAGAAGGAG 
GCCAGCTCCG TATCCTGGCA TCCCATACAC GAAGGCCTCT TCTGCTCCGG CGGCTCCGAT 
GGCTCCATTT TGTTTTGGAA TGTTGGTACC GACAAGGAGA TCGGCTGTGT GGAGACGGCA 
CACGACAGCA TCGTTTGGAC TCTGGCCTGG CACCCACTGG GTCACATTCT GTGCTCCGGC 
TCCAATGATC ATACGATCAA ATTCTGGACG AGGAACAGAC CGGGAGACCT GATGAGAGAC 
AAATACAATC TGAACACATT GCCCGCCAGC CTGGCTGCTC TGGATGAGTG TGAATACGAT 
GATGCAATTA TACCCGGAAT GGGACCCGAG GACCGGGTGG AGTTTACTGA AAGTTTAACT 
GCCGACAAAG GCTTTATTCC TGGCTTGGAT TTGGATCCAA GCAAAGCAAT AAATGACCGC 
GACCGGGAGA AAAAGGTTCC CTACAGCAAG CCCATTCCCC GCAATTTCCA GGTGAACTGG 
GCAGGACCCC GGCGAGCGGA CGATCCCAGC GTGCTCAGCA TTCACGAGGA GCTGGCCGCC 
CGAGAGGCCA AGGACACCAC CAATGGACTG AACCACCAGC AAATCAGCGA GAACACAATG 
GAGGGCATCC TGGCCAGCGG CATGCTCAAC GGCCTCGATC CACAGACCAT TGTCGGCGTT 
CTCGTGTACA ACCGGTTCAT TCGCGTTCAC CCGGACTCTC GACTGATGGC TGCTATTCGT 
CAGGGAGCCG AGTTTCTCAA CAAGTACATC GACGCTGGCA AGCTGGAGGA GCTCAACGAT 
GTAGTCCCAG TGAGGGATAG GTCCAGATCT GTATCACCCA CAGTGGAGGC ACGTGGGGAG 
ATGGTCTCCC CGCCCACCAA GAAGTCCCGC TTCGAACCAC GTCAGCATAA CGCCCAGTTG 
GTGTGCGTTC GAGAACTAAT GCAATGTAAA AAGCCCTTTC TGCAGTCACT GGTCGCGGCG 
AAAGCACAGC AACCCAACAC GGAGTTTGTG GATGAACCGC CGCTGGAGCA AAGGGATCGG 
GATCGCGAAC GAGAAGGAGA TCGCGACAGG GAGCGGGAGC GCGCTCGCGA ACGGGAGAGA 
GACCAATCCG GTGGCAGGCC CAATCCCTGG GCTTCCAATG CACCCTGGTC GAACAATGGC 
AATCCTGGTG GCAATGGCAA TGGATCCAAT GGCTTTAGCA ACAATGGCGC GGAAAGTTTC 
AACGACCGGG ATCGCGGTGG CGGTGGCCAA CGTCGCCGGC GTGGCATCAA CAACTCCGGT 
CCTGGCAACG GCGGTGGAAG TGGTGGAGGA GGTGGCGGGG GCAGTGGCCG AAATCGCGGT 
CGCAACAACA ACAGACGTTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730982.1
CDS	244..2667
Translation	MDVSQPPPQLLTAPSALATNFTSLPPPNMGGQHYRHYHPHHGSNKHGYNQFKPFMPGGFQRPFGMSQDDFDGKRLRKSVMRKTVDYNASIIKALENRLYQRDYRDRLALQPDSIYVPHMLPPSAYLDNPSNAVTTRFVKTATNKMRCPIFTLAWTPEGRRLVTGASSGEFTLWNGLTFNFETILQAHDISVRTMVWSHNDSWMVTGDHGGYVKYWQSNMNNVKMYQAHKEAIRGISFSPTDSKFVSGSDDGTLRIWDFMRCQEERVLRGHGADVKCVHWHPQKGMIVSGSKDNQQPIKIWDPKSGIALATLHAHKSTVMDLKWNDNGNWLVTASRDHLLKLFDIRNLREEVQVFRGHKKEASSVSWHPIHEGLFCSGGSDGSILFWNVGTDKEIGCVETAHDSIVWTLAWHPLGHILCSGSNDHTIKFWTRNRPGDLMRDKYNLNTLPASLAALDECEYDDAIIPGMGPEDRVEFTESLTADKGFIPGLDLDPSKAINDRDREKKVPYSKPIPRNFQVNWAGPRRADDPSVLSIHEELAAREAKDTTNGLNHQQISENTMEGILASGMLNGLDPQTIVGVLVYNRFIRVHPDSRLMAAIRQGAEFLNKYIDAGKLEELNDVVPVRDRSRSVSPTVEARGEMVSPPTKKSRFEPRQHNAQLVCVRELMQCKKPFLQSLVAAKAQQPNTEFVDEPPLEQRDRDREREGDRDRERERARERERDQSGGRPNPWASNAPWSNNGNPGGNGNGSNGFSNNGAESFNDRDRGGGGQRRRRGINNSGPGNGGGSGGGGGGGSGRNRGRNNNRRY

Target ORF information:

RefSeq Version	NM_169091.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WD repeat domain 33 (Wdr33), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169091.2

ORF Insert Sequence:

ATGGACGTAT CCCAGCCGCC ACCGCAGCTG CTCACGGCGC CCTCGGCCCT GGCCACCAAC 
TTCACATCGC TGCCCCCGCC GAACATGGGC GGCCAGCACT ACCGTCACTA CCATCCGCAC 
CATGGGTCCA ACAAGCACGG CTACAACCAG TTCAAACCCT TCATGCCCGG CGGCTTCCAG 
CGACCCTTCG GAATGTCCCA GGACGACTTC GACGGCAAGC GACTGCGAAA AAGTGTGATG 
CGGAAGACAG TTGACTACAA CGCGTCCATC ATCAAGGCAC TGGAGAACCG ATTGTACCAG 
CGGGACTATC GGGACCGTTT GGCTTTGCAG CCGGACAGCA TTTATGTGCC CCATATGCTG 
CCGCCCTCTG CCTACTTGGA CAATCCTTCG AACGCGGTGA CCACAAGGTT CGTGAAGACG 
GCTACCAATA AGATGCGATG TCCGATTTTC ACACTGGCCT GGACGCCAGA GGGCAGACGT 
TTGGTAACCG GGGCCAGTTC CGGCGAGTTT ACGCTATGGA ACGGACTAAC CTTTAATTTC 
GAGACCATTC TGCAGGCCCA CGACATTTCT GTGCGGACGA TGGTGTGGTC GCACAACGAC 
AGCTGGATGG TGACCGGCGA CCATGGCGGC TATGTTAAGT ACTGGCAGTC CAACATGAAC 
AACGTGAAGA TGTACCAGGC GCACAAGGAG GCGATTAGGG GAATAAGTTT CAGTCCCACG 
GACAGCAAGT TCGTCAGCGG CAGCGACGAC GGAACCCTGA GGATATGGGA CTTCATGCGG 
TGCCAGGAGG AGCGAGTGCT ACGAGGCCAC GGTGCTGACG TCAAGTGCGT GCACTGGCAT 
CCACAAAAGG GAATGATCGT CAGCGGAAGC AAGGACAACC AGCAACCCAT CAAGATTTGG 
GATCCCAAGA GCGGCATTGC CCTGGCTACT CTACACGCCC ACAAATCCAC CGTGATGGAT 
CTGAAGTGGA ACGACAATGG CAACTGGCTG GTAACCGCAT CCAGAGATCA TCTGCTCAAG 
CTCTTCGACA TTCGCAACCT GCGTGAGGAG GTTCAGGTCT TCCGTGGCCA CAAGAAGGAG 
GCCAGCTCCG TATCCTGGCA TCCCATACAC GAAGGCCTCT TCTGCTCCGG CGGCTCCGAT 
GGCTCCATTT TGTTTTGGAA TGTTGGTACC GACAAGGAGA TCGGCTGTGT GGAGACGGCA 
CACGACAGCA TCGTTTGGAC TCTGGCCTGG CACCCACTGG GTCACATTCT GTGCTCCGGC 
TCCAATGATC ATACGATCAA ATTCTGGACG AGGAACAGAC CGGGAGACCT GATGAGAGAC 
AAATACAATC TGAACACATT GCCCGCCAGC CTGGCTGCTC TGGATGAGTG TGAATACGAT 
GATGCAATTA TACCCGGAAT GGGACCCGAG GACCGGGTGG AGTTTACTGA AAGTTTAACT 
GCCGACAAAG GCTTTATTCC TGGCTTGGAT TTGGATCCAA GCAAAGCAAT AAATGACCGC 
GACCGGGAGA AAAAGGTTCC CTACAGCAAG CCCATTCCCC GCAATTTCCA GGTGAACTGG 
GCAGGACCCC GGCGAGCGGA CGATCCCAGC GTGCTCAGCA TTCACGAGGA GCTGGCCGCC 
CGAGAGGCCA AGGACACCAC CAATGGACTG AACCACCAGC AAATCAGCGA GAACACAATG 
GAGGGCATCC TGGCCAGCGG CATGCTCAAC GGCCTCGATC CACAGACCAT TGTCGGCGTT 
CTCGTGTACA ACCGGTTCAT TCGCGTTCAC CCGGACTCTC GACTGATGGC TGCTATTCGT 
CAGGGAGCCG AGTTTCTCAA CAAGTACATC GACGCTGGCA AGCTGGAGGA GCTCAACGAT 
GTAGTCCCAG TGAGGGATAG GTCCAGATCT GTATCACCCA CAGTGGAGGC ACGTGGGGAG 
ATGGTCTCCC CGCCCACCAA GAAGTCCCGC TTCGAACCAC GTCAGCATAA CGCCCAGTTG 
GTGTGCGTTC GAGAACTAAT GCAATGTAAA AAGCCCTTTC TGCAGTCACT GGTCGCGGCG 
AAAGCACAGC AACCCAACAC GGAGTTTGTG GATGAACCGC CGCTGGAGCA AAGGGATCGG 
GATCGCGAAC GAGAAGGAGA TCGCGACAGG GAGCGGGAGC GCGCTCGCGA ACGGGAGAGA 
GACCAATCCG GTGGCAGGCC CAATCCCTGG GCTTCCAATG CACCCTGGTC GAACAATGGC 
AATCCTGGTG GCAATGGCAA TGGATCCAAT GGCTTTAGCA ACAATGGCGC GGAAAGTTTC 
AACGACCGGG ATCGCGGTGG CGGTGGCCAA CGTCGCCGGC GTGGCATCAA CAACTCCGGT 
CCTGGCAACG GCGGTGGAAG TGGTGGAGGA GGTGGCGGGG GCAGTGGCCG AAATCGCGGT 
CGCAACAACA ACAGACGTTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wdr33 ( NM_169092.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730983.1 Drosophila melanogaster WD repeat domain 33 (Wdr33), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03980
Clone ID Related Accession (Same CDS sequence)	NM_169091.2 , NM_169092.2
Accession Version	NM_169092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WD repeat domain 33, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169092.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGTAT CCCAGCCGCC ACCGCAGCTG CTCACGGCGC CCTCGGCCCT GGCCACCAAC 
TTCACATCGC TGCCCCCGCC GAACATGGGC GGCCAGCACT ACCGTCACTA CCATCCGCAC 
CATGGGTCCA ACAAGCACGG CTACAACCAG TTCAAACCCT TCATGCCCGG CGGCTTCCAG 
CGACCCTTCG GAATGTCCCA GGACGACTTC GACGGCAAGC GACTGCGAAA AAGTGTGATG 
CGGAAGACAG TTGACTACAA CGCGTCCATC ATCAAGGCAC TGGAGAACCG ATTGTACCAG 
CGGGACTATC GGGACCGTTT GGCTTTGCAG CCGGACAGCA TTTATGTGCC CCATATGCTG 
CCGCCCTCTG CCTACTTGGA CAATCCTTCG AACGCGGTGA CCACAAGGTT CGTGAAGACG 
GCTACCAATA AGATGCGATG TCCGATTTTC ACACTGGCCT GGACGCCAGA GGGCAGACGT 
TTGGTAACCG GGGCCAGTTC CGGCGAGTTT ACGCTATGGA ACGGACTAAC CTTTAATTTC 
GAGACCATTC TGCAGGCCCA CGACATTTCT GTGCGGACGA TGGTGTGGTC GCACAACGAC 
AGCTGGATGG TGACCGGCGA CCATGGCGGC TATGTTAAGT ACTGGCAGTC CAACATGAAC 
AACGTGAAGA TGTACCAGGC GCACAAGGAG GCGATTAGGG GAATAAGTTT CAGTCCCACG 
GACAGCAAGT TCGTCAGCGG CAGCGACGAC GGAACCCTGA GGATATGGGA CTTCATGCGG 
TGCCAGGAGG AGCGAGTGCT ACGAGGCCAC GGTGCTGACG TCAAGTGCGT GCACTGGCAT 
CCACAAAAGG GAATGATCGT CAGCGGAAGC AAGGACAACC AGCAACCCAT CAAGATTTGG 
GATCCCAAGA GCGGCATTGC CCTGGCTACT CTACACGCCC ACAAATCCAC CGTGATGGAT 
CTGAAGTGGA ACGACAATGG CAACTGGCTG GTAACCGCAT CCAGAGATCA TCTGCTCAAG 
CTCTTCGACA TTCGCAACCT GCGTGAGGAG GTTCAGGTCT TCCGTGGCCA CAAGAAGGAG 
GCCAGCTCCG TATCCTGGCA TCCCATACAC GAAGGCCTCT TCTGCTCCGG CGGCTCCGAT 
GGCTCCATTT TGTTTTGGAA TGTTGGTACC GACAAGGAGA TCGGCTGTGT GGAGACGGCA 
CACGACAGCA TCGTTTGGAC TCTGGCCTGG CACCCACTGG GTCACATTCT GTGCTCCGGC 
TCCAATGATC ATACGATCAA ATTCTGGACG AGGAACAGAC CGGGAGACCT GATGAGAGAC 
AAATACAATC TGAACACATT GCCCGCCAGC CTGGCTGCTC TGGATGAGTG TGAATACGAT 
GATGCAATTA TACCCGGAAT GGGACCCGAG GACCGGGTGG AGTTTACTGA AAGTTTAACT 
GCCGACAAAG GCTTTATTCC TGGCTTGGAT TTGGATCCAA GCAAAGCAAT AAATGACCGC 
GACCGGGAGA AAAAGGTTCC CTACAGCAAG CCCATTCCCC GCAATTTCCA GGTGAACTGG 
GCAGGACCCC GGCGAGCGGA CGATCCCAGC GTGCTCAGCA TTCACGAGGA GCTGGCCGCC 
CGAGAGGCCA AGGACACCAC CAATGGACTG AACCACCAGC AAATCAGCGA GAACACAATG 
GAGGGCATCC TGGCCAGCGG CATGCTCAAC GGCCTCGATC CACAGACCAT TGTCGGCGTT 
CTCGTGTACA ACCGGTTCAT TCGCGTTCAC CCGGACTCTC GACTGATGGC TGCTATTCGT 
CAGGGAGCCG AGTTTCTCAA CAAGTACATC GACGCTGGCA AGCTGGAGGA GCTCAACGAT 
GTAGTCCCAG TGAGGGATAG GTCCAGATCT GTATCACCCA CAGTGGAGGC ACGTGGGGAG 
ATGGTCTCCC CGCCCACCAA GAAGTCCCGC TTCGAACCAC GTCAGCATAA CGCCCAGTTG 
GTGTGCGTTC GAGAACTAAT GCAATGTAAA AAGCCCTTTC TGCAGTCACT GGTCGCGGCG 
AAAGCACAGC AACCCAACAC GGAGTTTGTG GATGAACCGC CGCTGGAGCA AAGGGATCGG 
GATCGCGAAC GAGAAGGAGA TCGCGACAGG GAGCGGGAGC GCGCTCGCGA ACGGGAGAGA 
GACCAATCCG GTGGCAGGCC CAATCCCTGG GCTTCCAATG CACCCTGGTC GAACAATGGC 
AATCCTGGTG GCAATGGCAA TGGATCCAAT GGCTTTAGCA ACAATGGCGC GGAAAGTTTC 
AACGACCGGG ATCGCGGTGG CGGTGGCCAA CGTCGCCGGC GTGGCATCAA CAACTCCGGT 
CCTGGCAACG GCGGTGGAAG TGGTGGAGGA GGTGGCGGGG GCAGTGGCCG AAATCGCGGT 
CGCAACAACA ACAGACGTTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730983.1
CDS	231..2654
Translation	MDVSQPPPQLLTAPSALATNFTSLPPPNMGGQHYRHYHPHHGSNKHGYNQFKPFMPGGFQRPFGMSQDDFDGKRLRKSVMRKTVDYNASIIKALENRLYQRDYRDRLALQPDSIYVPHMLPPSAYLDNPSNAVTTRFVKTATNKMRCPIFTLAWTPEGRRLVTGASSGEFTLWNGLTFNFETILQAHDISVRTMVWSHNDSWMVTGDHGGYVKYWQSNMNNVKMYQAHKEAIRGISFSPTDSKFVSGSDDGTLRIWDFMRCQEERVLRGHGADVKCVHWHPQKGMIVSGSKDNQQPIKIWDPKSGIALATLHAHKSTVMDLKWNDNGNWLVTASRDHLLKLFDIRNLREEVQVFRGHKKEASSVSWHPIHEGLFCSGGSDGSILFWNVGTDKEIGCVETAHDSIVWTLAWHPLGHILCSGSNDHTIKFWTRNRPGDLMRDKYNLNTLPASLAALDECEYDDAIIPGMGPEDRVEFTESLTADKGFIPGLDLDPSKAINDRDREKKVPYSKPIPRNFQVNWAGPRRADDPSVLSIHEELAAREAKDTTNGLNHQQISENTMEGILASGMLNGLDPQTIVGVLVYNRFIRVHPDSRLMAAIRQGAEFLNKYIDAGKLEELNDVVPVRDRSRSVSPTVEARGEMVSPPTKKSRFEPRQHNAQLVCVRELMQCKKPFLQSLVAAKAQQPNTEFVDEPPLEQRDRDREREGDRDRERERARERERDQSGGRPNPWASNAPWSNNGNPGGNGNGSNGFSNNGAESFNDRDRGGGGQRRRRGINNSGPGNGGGSGGGGGGGSGRNRGRNNNRRY

Target ORF information:

RefSeq Version	NM_169092.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WD repeat domain 33 (Wdr33), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169092.2

ORF Insert Sequence:

ATGGACGTAT CCCAGCCGCC ACCGCAGCTG CTCACGGCGC CCTCGGCCCT GGCCACCAAC 
TTCACATCGC TGCCCCCGCC GAACATGGGC GGCCAGCACT ACCGTCACTA CCATCCGCAC 
CATGGGTCCA ACAAGCACGG CTACAACCAG TTCAAACCCT TCATGCCCGG CGGCTTCCAG 
CGACCCTTCG GAATGTCCCA GGACGACTTC GACGGCAAGC GACTGCGAAA AAGTGTGATG 
CGGAAGACAG TTGACTACAA CGCGTCCATC ATCAAGGCAC TGGAGAACCG ATTGTACCAG 
CGGGACTATC GGGACCGTTT GGCTTTGCAG CCGGACAGCA TTTATGTGCC CCATATGCTG 
CCGCCCTCTG CCTACTTGGA CAATCCTTCG AACGCGGTGA CCACAAGGTT CGTGAAGACG 
GCTACCAATA AGATGCGATG TCCGATTTTC ACACTGGCCT GGACGCCAGA GGGCAGACGT 
TTGGTAACCG GGGCCAGTTC CGGCGAGTTT ACGCTATGGA ACGGACTAAC CTTTAATTTC 
GAGACCATTC TGCAGGCCCA CGACATTTCT GTGCGGACGA TGGTGTGGTC GCACAACGAC 
AGCTGGATGG TGACCGGCGA CCATGGCGGC TATGTTAAGT ACTGGCAGTC CAACATGAAC 
AACGTGAAGA TGTACCAGGC GCACAAGGAG GCGATTAGGG GAATAAGTTT CAGTCCCACG 
GACAGCAAGT TCGTCAGCGG CAGCGACGAC GGAACCCTGA GGATATGGGA CTTCATGCGG 
TGCCAGGAGG AGCGAGTGCT ACGAGGCCAC GGTGCTGACG TCAAGTGCGT GCACTGGCAT 
CCACAAAAGG GAATGATCGT CAGCGGAAGC AAGGACAACC AGCAACCCAT CAAGATTTGG 
GATCCCAAGA GCGGCATTGC CCTGGCTACT CTACACGCCC ACAAATCCAC CGTGATGGAT 
CTGAAGTGGA ACGACAATGG CAACTGGCTG GTAACCGCAT CCAGAGATCA TCTGCTCAAG 
CTCTTCGACA TTCGCAACCT GCGTGAGGAG GTTCAGGTCT TCCGTGGCCA CAAGAAGGAG 
GCCAGCTCCG TATCCTGGCA TCCCATACAC GAAGGCCTCT TCTGCTCCGG CGGCTCCGAT 
GGCTCCATTT TGTTTTGGAA TGTTGGTACC GACAAGGAGA TCGGCTGTGT GGAGACGGCA 
CACGACAGCA TCGTTTGGAC TCTGGCCTGG CACCCACTGG GTCACATTCT GTGCTCCGGC 
TCCAATGATC ATACGATCAA ATTCTGGACG AGGAACAGAC CGGGAGACCT GATGAGAGAC 
AAATACAATC TGAACACATT GCCCGCCAGC CTGGCTGCTC TGGATGAGTG TGAATACGAT 
GATGCAATTA TACCCGGAAT GGGACCCGAG GACCGGGTGG AGTTTACTGA AAGTTTAACT 
GCCGACAAAG GCTTTATTCC TGGCTTGGAT TTGGATCCAA GCAAAGCAAT AAATGACCGC 
GACCGGGAGA AAAAGGTTCC CTACAGCAAG CCCATTCCCC GCAATTTCCA GGTGAACTGG 
GCAGGACCCC GGCGAGCGGA CGATCCCAGC GTGCTCAGCA TTCACGAGGA GCTGGCCGCC 
CGAGAGGCCA AGGACACCAC CAATGGACTG AACCACCAGC AAATCAGCGA GAACACAATG 
GAGGGCATCC TGGCCAGCGG CATGCTCAAC GGCCTCGATC CACAGACCAT TGTCGGCGTT 
CTCGTGTACA ACCGGTTCAT TCGCGTTCAC CCGGACTCTC GACTGATGGC TGCTATTCGT 
CAGGGAGCCG AGTTTCTCAA CAAGTACATC GACGCTGGCA AGCTGGAGGA GCTCAACGAT 
GTAGTCCCAG TGAGGGATAG GTCCAGATCT GTATCACCCA CAGTGGAGGC ACGTGGGGAG 
ATGGTCTCCC CGCCCACCAA GAAGTCCCGC TTCGAACCAC GTCAGCATAA CGCCCAGTTG 
GTGTGCGTTC GAGAACTAAT GCAATGTAAA AAGCCCTTTC TGCAGTCACT GGTCGCGGCG 
AAAGCACAGC AACCCAACAC GGAGTTTGTG GATGAACCGC CGCTGGAGCA AAGGGATCGG 
GATCGCGAAC GAGAAGGAGA TCGCGACAGG GAGCGGGAGC GCGCTCGCGA ACGGGAGAGA 
GACCAATCCG GTGGCAGGCC CAATCCCTGG GCTTCCAATG CACCCTGGTC GAACAATGGC 
AATCCTGGTG GCAATGGCAA TGGATCCAAT GGCTTTAGCA ACAATGGCGC GGAAAGTTTC 
AACGACCGGG ATCGCGGTGG CGGTGGCCAA CGTCGCCGGC GTGGCATCAA CAACTCCGGT 
CCTGGCAACG GCGGTGGAAG TGGTGGAGGA GGTGGCGGGG GCAGTGGCCG AAATCGCGGT 
CGCAACAACA ACAGACGTTA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Wdr33 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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