NKCC cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NKCC
Entrez Gene ID	40663
Full Name	CG31547 gene product from transcript CG31547-RB
Synonyms	CG10544,CG2509,Dmel\CG31547
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83A5-83A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_169057.1	NP_730938.1	sodium potassium chloride cotransporter, isoform B
NM_169058.1	NP_730939.1	sodium potassium chloride cotransporter, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003275	XP_560491.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003274	XP_001687817.2
Drosophila melanogaster (fruit fly)	CG31547	NP_730938.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NKCC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKCC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03940	NM_169058.1 Latest version!	Drosophila melanogaster sodium potassium chloride cotransporter (NKCC), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03939	NM_169057.1 Latest version!	Drosophila melanogaster sodium potassium chloride cotransporter (NKCC), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NKCC ( NM_169058.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730939.1 Drosophila melanogaster sodium potassium chloride cotransporter (NKCC), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03940
Clone ID Related Accession (Same CDS sequence)	NM_169058.1
Accession Version	NM_169058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium potassium chloride cotransporter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCCCG AGGCGGTGAT AGTGCTGCTT CCGTTCCACC AGGAGAGTCT GCCGCGCCTG 
GACAACTACC GCATTTCCAT GCGCAACCTC AAGCGTCCTT CTATCGGCGA ACTTCAAGGA 
GAGGCGGTCG ACCAGAGCAT CACTATTCCC GAACCCGAAC CGGAGGCAAC TGGCGGACAC 
ATCAAGCTAG GATGGATCGT GGGCGTGCTG ATACCTTGCC TTCTCAACAT CTGGGGCGTA 
ATGCTCTTCC TGCGCCTCAG CTGGGTGGTG GCCGAGTCGG GCATCCTGCA GTCCCTGATT 
ATAATTACGA TCTCCGCGGT GGTCTGCGTC ATCACGACGC TCTCGCTGTC GGCCATTAGC 
ACCAACGGCG AGGTCAAGGG CGGCGGCGTC TACTTCATCA TATCCCGTTC GCTGGGCCCC 
GAGTTTGGGG CGTCGGTGGG CGTGGTCTTC GCCTTCGCCA ACGCCGTCTC AGCCTCGATG 
AACACCATCG GCTTCTGCGA ATCGCTAAAT GTTTTACTCA AGAACAACGA CCTGAAGATT 
GTCGACAACG GGATCAATGA CATCCGGATC GTGGGATCGA TAACCGTGCT GGTGCTCATC 
CTAATCTGCT GCGTGGGTAT GGAGTGGGAG ACGAAGGCGC AGAACTTCCT GATCGTCACG 
ATTGTCCTGG CCATCTTCAA TTTCCTGATC GGAGCTGCCA TTGGACCCCA GGGTAATGAG 
GAGCAAATCT CTAGGGGTTT CGTCGGCTTT TCATGGGCTA CTTTGAAGGA GAACTTCGGA 
TCGGACTATC GCTATGCGGA GGGCGTAAAC CACGACTTTT TCAGCGTGTT CGCTATCTTC 
TTTCCCAGCG TGACGGGCAT CCAGGCAGGA GCAAATATCT GCGGAGACCT CAAGGATGCC 
GGAGCTGCTA TACCGAAGGG CACCTTCTGG TCCCTGCTCA TCTCGATGTC CTCATACGCT 
CTCTTTGTCC TCTTCGCCGG TGGCGCTGCT GTAAGAGATG CCTCCGGTAT ACCAGCTGAC 
CTCGTCAATG GCACCATTGT TTCCTCGGAG CTGCCGTGCA TGGCCACGGG CAACTGCACC 
TGGGGTCTCT TCAACTCGTA CGAAATGATG CAGGAGATGT CGCTGTGGGG ACCACTCATC 
TACGCCGGCT GCTTTGCAGC CACGTTAAGC ACGGCCCTGA CCAACTTGCT TTCCGTTCCC 
AGGCTGGTGC AGGCCCTGGG CATTGACCAG ATCTACCCGG GTCTAATCTT CTTCTCGAAG 
CCGTACGGCA AGCACGGCGA ACCGTATCGC GGCTACGTGC TCACCTTCTT CATCACCACC 
GGCTTCCTGC TGATCGGAGA GCTTAACCTG ATCGCTCCTC TGATCTCCAC CTTCTACCTG 
GCCTCCTACG CCCTCATCAA CTTTTGCACA TTCCACGCGG CCTTCGTGAA GCCATTGGGA 
TGGCGACCCA CCTTTAAGTA CTACAACGCC TGGCTCAGCC TCTTCGGGTT CGCCATGTGC 
GTGGCCATCA TGTTCCTGAT CAACTACGTG GCCGCCATCA TCACCTTTGG CATCATCTTC 
GCCCTCTATC TGGTGGTCAT GTACCGCAAG CCGGAGGCCA ACTGGGGATC CACCACGCAG 
GCTCAGCAGT ACAAGGCTGC TCTAATGGCC GTCCACCGAC TGCAGAACGT CTCGGATCAC 
GTCAAGAACT ACCATCCCCA GGTGCTGGTG CTCTCCGGCG ATCCCAAGAC CAGACCGCCA 
CTCGTGGACT TTGGCTACCT GCTGACCAAG AACAACTCCC TCATGTTCGT GGCAAATATA 
ATACCGGTGC GGGTGGGCTA CAAGAACCGG CAGCACCTGG TGAAGGATGG ACAAAAGTAC 
CTGGATGCCC GCAAGATCAA GGCCTTCTAC AATGTGATTG ATGGGTTCAG CCTGGAGGAC 
GGAATAAATG CGTTGACAAA GTCAACGGGC TTCGGCAAAA TGTCTCCGAA CATTGTGCTG 
GTGGGCTACA AGCCGGACTG GAATCGCTGT CGCAAGGAGG AGGTGGAGAG TTACTTTTCC 
ATTTTGTACA ATGCTTTTTC TCAGCGTATG GGCGTGGCCC TGCTCCGACT GCCCAACGGA 
TTGGATTTCT CTGAGCTGAG TTCCGAGGTC ACTCTGCCGG CCAACGGAAT GGGTCACATG 
CATACCGCCA ATGCGCATGG ATTCACCAAC GAACTGATGC CGGCGGCCAA TGCAGCCTCG 
GAGCTGCTGC ACATTGACTC CAACCTGAAC CTAGCCAGCA TGGACAGCCC CAACTCCTCT 
TTCACAATGC CCCAGCCAGC ACCGATGCCC AACATGCAGC GCAACTCCCG CAGCTACAAG 
GTGACCAGCT CGGATGAGCC CGCCGTCACC TACCACACCA AGGGCGGATC GGACATACCC 
CAAAACTTGC TGGACGCCAT GACCATATTC ACGCGCAAAC AGCCCAAGGG CACAATCGAT 
GTCTTCTGGC TGTACGACGA CGGAGGTCTC ACAATTCTCC TGCCCTACAT AATCTCCATG 
CGCTCCCACT GGCAGAACTC CAAGCTAAGG GTGTTCGCAA TGTGCCACGG AAAAGACGAG 
GAGCAGGAAG AGAAGAGCAT GGCTAGCTTG TTGACCAAGT TCCGCATCAA GTACTCTGAG 
CTCATTATGC TGAAGGGCGT CTCCGAGCAG CCGCGGGCGG ACACGGTGCT GAAGCACAAG 
CGGCTGATCG AGCCCTTCCG CCGCGGAGCC CGCAACGAGT TCGGCATCAC GGACGATGAG 
CTGCAGAGCA TGTCGGAGAA GACGAATCGC CAGCTGCGCA TCCATGAGCT GGTGGTCAAG 
CACTCATCAA ACGCTTCCCT GGTGGTCATG TCCCTGCCCA TGCCGCGCAA GGAGGCCATC 
TCGGCACCGC TGTACATGTC ATGGCTGGAG ATGCTCACGT CGGACATGAA GTGTCCGGTG 
GCATTGGCGC GCGGCAACCA GACGCCCGTG CTGACGCTCT ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730939.1
CDS	32..3019
Translation	MLPEAVIVLLPFHQESLPRLDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPFRRGARNEFGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS

Target ORF information:

RefSeq Version	NM_169058.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium potassium chloride cotransporter (NKCC), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169058.1

ORF Insert Sequence:

ATGCTGCCCG AGGCGGTGAT AGTGCTGCTT CCGTTCCACC AGGAGAGTCT GCCGCGCCTG 
GACAACTACC GCATTTCCAT GCGCAACCTC AAGCGTCCTT CTATCGGCGA ACTTCAAGGA 
GAGGCGGTCG ACCAGAGCAT CACTATTCCC GAACCCGAAC CGGAGGCAAC TGGCGGACAC 
ATCAAGCTAG GATGGATCGT GGGCGTGCTG ATACCTTGCC TTCTCAACAT CTGGGGCGTA 
ATGCTCTTCC TGCGCCTCAG CTGGGTGGTG GCCGAGTCGG GCATCCTGCA GTCCCTGATT 
ATAATTACGA TCTCCGCGGT GGTCTGCGTC ATCACGACGC TCTCGCTGTC GGCCATTAGC 
ACCAACGGCG AGGTCAAGGG CGGCGGCGTC TACTTCATCA TATCCCGTTC GCTGGGCCCC 
GAGTTTGGGG CGTCGGTGGG CGTGGTCTTC GCCTTCGCCA ACGCCGTCTC AGCCTCGATG 
AACACCATCG GCTTCTGCGA ATCGCTAAAT GTTTTACTCA AGAACAACGA CCTGAAGATT 
GTCGACAACG GGATCAATGA CATCCGGATC GTGGGATCGA TAACCGTGCT GGTGCTCATC 
CTAATCTGCT GCGTGGGTAT GGAGTGGGAG ACGAAGGCGC AGAACTTCCT GATCGTCACG 
ATTGTCCTGG CCATCTTCAA TTTCCTGATC GGAGCTGCCA TTGGACCCCA GGGTAATGAG 
GAGCAAATCT CTAGGGGTTT CGTCGGCTTT TCATGGGCTA CTTTGAAGGA GAACTTCGGA 
TCGGACTATC GCTATGCGGA GGGCGTAAAC CACGACTTTT TCAGCGTGTT CGCTATCTTC 
TTTCCCAGCG TGACGGGCAT CCAGGCAGGA GCAAATATCT GCGGAGACCT CAAGGATGCC 
GGAGCTGCTA TACCGAAGGG CACCTTCTGG TCCCTGCTCA TCTCGATGTC CTCATACGCT 
CTCTTTGTCC TCTTCGCCGG TGGCGCTGCT GTAAGAGATG CCTCCGGTAT ACCAGCTGAC 
CTCGTCAATG GCACCATTGT TTCCTCGGAG CTGCCGTGCA TGGCCACGGG CAACTGCACC 
TGGGGTCTCT TCAACTCGTA CGAAATGATG CAGGAGATGT CGCTGTGGGG ACCACTCATC 
TACGCCGGCT GCTTTGCAGC CACGTTAAGC ACGGCCCTGA CCAACTTGCT TTCCGTTCCC 
AGGCTGGTGC AGGCCCTGGG CATTGACCAG ATCTACCCGG GTCTAATCTT CTTCTCGAAG 
CCGTACGGCA AGCACGGCGA ACCGTATCGC GGCTACGTGC TCACCTTCTT CATCACCACC 
GGCTTCCTGC TGATCGGAGA GCTTAACCTG ATCGCTCCTC TGATCTCCAC CTTCTACCTG 
GCCTCCTACG CCCTCATCAA CTTTTGCACA TTCCACGCGG CCTTCGTGAA GCCATTGGGA 
TGGCGACCCA CCTTTAAGTA CTACAACGCC TGGCTCAGCC TCTTCGGGTT CGCCATGTGC 
GTGGCCATCA TGTTCCTGAT CAACTACGTG GCCGCCATCA TCACCTTTGG CATCATCTTC 
GCCCTCTATC TGGTGGTCAT GTACCGCAAG CCGGAGGCCA ACTGGGGATC CACCACGCAG 
GCTCAGCAGT ACAAGGCTGC TCTAATGGCC GTCCACCGAC TGCAGAACGT CTCGGATCAC 
GTCAAGAACT ACCATCCCCA GGTGCTGGTG CTCTCCGGCG ATCCCAAGAC CAGACCGCCA 
CTCGTGGACT TTGGCTACCT GCTGACCAAG AACAACTCCC TCATGTTCGT GGCAAATATA 
ATACCGGTGC GGGTGGGCTA CAAGAACCGG CAGCACCTGG TGAAGGATGG ACAAAAGTAC 
CTGGATGCCC GCAAGATCAA GGCCTTCTAC AATGTGATTG ATGGGTTCAG CCTGGAGGAC 
GGAATAAATG CGTTGACAAA GTCAACGGGC TTCGGCAAAA TGTCTCCGAA CATTGTGCTG 
GTGGGCTACA AGCCGGACTG GAATCGCTGT CGCAAGGAGG AGGTGGAGAG TTACTTTTCC 
ATTTTGTACA ATGCTTTTTC TCAGCGTATG GGCGTGGCCC TGCTCCGACT GCCCAACGGA 
TTGGATTTCT CTGAGCTGAG TTCCGAGGTC ACTCTGCCGG CCAACGGAAT GGGTCACATG 
CATACCGCCA ATGCGCATGG ATTCACCAAC GAACTGATGC CGGCGGCCAA TGCAGCCTCG 
GAGCTGCTGC ACATTGACTC CAACCTGAAC CTAGCCAGCA TGGACAGCCC CAACTCCTCT 
TTCACAATGC CCCAGCCAGC ACCGATGCCC AACATGCAGC GCAACTCCCG CAGCTACAAG 
GTGACCAGCT CGGATGAGCC CGCCGTCACC TACCACACCA AGGGCGGATC GGACATACCC 
CAAAACTTGC TGGACGCCAT GACCATATTC ACGCGCAAAC AGCCCAAGGG CACAATCGAT 
GTCTTCTGGC TGTACGACGA CGGAGGTCTC ACAATTCTCC TGCCCTACAT AATCTCCATG 
CGCTCCCACT GGCAGAACTC CAAGCTAAGG GTGTTCGCAA TGTGCCACGG AAAAGACGAG 
GAGCAGGAAG AGAAGAGCAT GGCTAGCTTG TTGACCAAGT TCCGCATCAA GTACTCTGAG 
CTCATTATGC TGAAGGGCGT CTCCGAGCAG CCGCGGGCGG ACACGGTGCT GAAGCACAAG 
CGGCTGATCG AGCCCTTCCG CCGCGGAGCC CGCAACGAGT TCGGCATCAC GGACGATGAG 
CTGCAGAGCA TGTCGGAGAA GACGAATCGC CAGCTGCGCA TCCATGAGCT GGTGGTCAAG 
CACTCATCAA ACGCTTCCCT GGTGGTCATG TCCCTGCCCA TGCCGCGCAA GGAGGCCATC 
TCGGCACCGC TGTACATGTC ATGGCTGGAG ATGCTCACGT CGGACATGAA GTGTCCGGTG 
GCATTGGCGC GCGGCAACCA GACGCCCGTG CTGACGCTCT ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKCC ( NM_169057.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730938.1 Drosophila melanogaster sodium potassium chloride cotransporter (NKCC), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03939
Clone ID Related Accession (Same CDS sequence)	NM_169057.1
Accession Version	NM_169057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sodium potassium chloride cotransporter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGATC GCTTCCAGAT CTCAAAGGTG AACGGCACCG CGAACGCCGG CTACGATGGC 
GAGGCTAGAT CAGCTGAATC CGACCAGACG GACGCAGCCC ACCCACTTCC GCCCACCAGC 
AACGAAAACC ACCTGCATCC CAAGGCTTCG GGGGAGACGG GCGAGAACCG CCGCAGCTCC 
CGGCTCTCCT TCCGCGGATT CGGTAACTTC CTGCGCAAAT CGGACGCCGA GCGCAAGTTT 
AGTCTCGCCC AGCTAACAAA GGAGAGTCTG CCGCGCCTGG ACAACTACCG CATTTCCATG 
CGCAACCTCA AGCGTCCTTC TATCGGCGAA CTTCAAGGAG AGGCGGTCGA CCAGAGCATC 
ACTATTCCCG AACCCGAACC GGAGGCAACT GGCGGACACA TCAAGCTAGG ATGGATCGTG 
GGCGTGCTGA TACCTTGCCT TCTCAACATC TGGGGCGTAA TGCTCTTCCT GCGCCTCAGC 
TGGGTGGTGG CCGAGTCGGG CATCCTGCAG TCCCTGATTA TAATTACGAT CTCCGCGGTG 
GTCTGCGTCA TCACGACGCT CTCGCTGTCG GCCATTAGCA CCAACGGCGA GGTCAAGGGC 
GGCGGCGTCT ACTTCATCAT ATCCCGTTCG CTGGGCCCCG AGTTTGGGGC GTCGGTGGGC 
GTGGTCTTCG CCTTCGCCAA CGCCGTCTCA GCCTCGATGA ACACCATCGG CTTCTGCGAA 
TCGCTAAATG TTTTACTCAA GAACAACGAC CTGAAGATTG TCGACAACGG GATCAATGAC 
ATCCGGATCG TGGGATCGAT AACCGTGCTG GTGCTCATCC TAATCTGCTG CGTGGGTATG 
GAGTGGGAGA CGAAGGCGCA GAACTTCCTG ATCGTCACGA TTGTCCTGGC CATCTTCAAT 
TTCCTGATCG GAGCTGCCAT TGGACCCCAG GGTAATGAGG AGCAAATCTC TAGGGGTTTC 
GTCGGCTTTT CATGGGCTAC TTTGAAGGAG AACTTCGGAT CGGACTATCG CTATGCGGAG 
GGCGTAAACC ACGACTTTTT CAGCGTGTTC GCTATCTTCT TTCCCAGCGT GACGGGCATC 
CAGGCAGGAG CAAATATCTG CGGAGACCTC AAGGATGCCG GAGCTGCTAT ACCGAAGGGC 
ACCTTCTGGT CCCTGCTCAT CTCGATGTCC TCATACGCTC TCTTTGTCCT CTTCGCCGGT 
GGCGCTGCTG TAAGAGATGC CTCCGGTATA CCAGCTGACC TCGTCAATGG CACCATTGTT 
TCCTCGGAGC TGCCGTGCAT GGCCACGGGC AACTGCACCT GGGGTCTCTT CAACTCGTAC 
GAAATGATGC AGGAGATGTC GCTGTGGGGA CCACTCATCT ACGCCGGCTG CTTTGCAGCC 
ACGTTAAGCA CGGCCCTGAC CAACTTGCTT TCCGTTCCCA GGCTGGTGCA GGCCCTGGGC 
ATTGACCAGA TCTACCCGGG TCTAATCTTC TTCTCGAAGC CGTACGGCAA GCACGGCGAA 
CCGTATCGCG GCTACGTGCT CACCTTCTTC ATCACCACCG GCTTCCTGCT GATCGGAGAG 
CTTAACCTGA TCGCTCCTCT GATCTCCACC TTCTACCTGG CCTCCTACGC CCTCATCAAC 
TTTTGCACAT TCCACGCGGC CTTCGTGAAG CCATTGGGAT GGCGACCCAC CTTTAAGTAC 
TACAACGCCT GGCTCAGCCT CTTCGGGTTC GCCATGTGCG TGGCCATCAT GTTCCTGATC 
AACTACGTGG CCGCCATCAT CACCTTTGGC ATCATCTTCG CCCTCTATCT GGTGGTCATG 
TACCGCAAGC CGGAGGCCAA CTGGGGATCC ACCACGCAGG CTCAGCAGTA CAAGGCTGCT 
CTAATGGCCG TCCACCGACT GCAGAACGTC TCGGATCACG TCAAGAACTA CCATCCCCAG 
GTGCTGGTGC TCTCCGGCGA TCCCAAGACC AGACCGCCAC TCGTGGACTT TGGCTACCTG 
CTGACCAAGA ACAACTCCCT CATGTTCGTG GCAAATATAA TACCGGTGCG GGTGGGCTAC 
AAGAACCGGC AGCACCTGGT GAAGGATGGA CAAAAGTACC TGGATGCCCG CAAGATCAAG 
GCCTTCTACA ATGTGATTGA TGGGTTCAGC CTGGAGGACG GAATAAATGC GTTGACAAAG 
TCAACGGGCT TCGGCAAAAT GTCTCCGAAC ATTGTGCTGG TGGGCTACAA GCCGGACTGG 
AATCGCTGTC GCAAGGAGGA GGTGGAGAGT TACTTTTCCA TTTTGTACAA TGCTTTTTCT 
CAGCGTATGG GCGTGGCCCT GCTCCGACTG CCCAACGGAT TGGATTTCTC TGAGCTGAGT 
TCCGAGGTCA CTCTGCCGGC CAACGGAATG GGTCACATGC ATACCGCCAA TGCGCATGGA 
TTCACCAACG AACTGATGCC GGCGGCCAAT GCAGCCTCGG AGCTGCTGCA CATTGACTCC 
AACCTGAACC TAGCCAGCAT GGACAGCCCC AACTCCTCTT TCACAATGCC CCAGCCAGCA 
CCGATGCCCA ACATGCAGCG CAACTCCCGC AGCTACAAGG TGACCAGCTC GGATGAGCCC 
GCCGTCACCT ACCACACCAA GGGCGGATCG GACATACCCC AAAACTTGCT GGACGCCATG 
ACCATATTCA CGCGCAAACA GCCCAAGGGC ACAATCGATG TCTTCTGGCT GTACGACGAC 
GGAGGTCTCA CAATTCTCCT GCCCTACATA ATCTCCATGC GCTCCCACTG GCAGAACTCC 
AAGCTAAGGG TGTTCGCAAT GTGCCACGGA AAAGACGAGG AGCAGGAAGA GAAGAGCATG 
GCTAGCTTGT TGACCAAGTT CCGCATCAAG TACTCTGAGC TCATTATGCT GAAGGGCGTC 
TCCGAGCAGC CGCGGGCGGA CACGGTGCTG AAGCACAAGC GGCTGATCGA GCCCTTCCGC 
CGCGGAGCCC GCAACGAGTT CGGCATCACG GACGATGAGC TGCAGAGCAT GTCGGAGAAG 
ACGAATCGCC AGCTGCGCAT CCATGAGCTG GTGGTCAAGC ACTCATCAAA CGCTTCCCTG 
GTGGTCATGT CCCTGCCCAT GCCGCGCAAG GAGGCCATCT CGGCACCGCT GTACATGTCA 
TGGCTGGAGA TGCTCACGTC GGACATGAAG TGTCCGGTGG CATTGGCGCG CGGCAACCAG 
ACGCCCGTGC TGACGCTCTA CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730938.1
CDS	407..3613
Translation	MADRFQISKVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLSFRGFGNFLRKSDAERKFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPFRRGARNEFGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS

Target ORF information:

RefSeq Version	NM_169057.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sodium potassium chloride cotransporter (NKCC), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169057.1

ORF Insert Sequence:

ATGGCCGATC GCTTCCAGAT CTCAAAGGTG AACGGCACCG CGAACGCCGG CTACGATGGC 
GAGGCTAGAT CAGCTGAATC CGACCAGACG GACGCAGCCC ACCCACTTCC GCCCACCAGC 
AACGAAAACC ACCTGCATCC CAAGGCTTCG GGGGAGACGG GCGAGAACCG CCGCAGCTCC 
CGGCTCTCCT TCCGCGGATT CGGTAACTTC CTGCGCAAAT CGGACGCCGA GCGCAAGTTT 
AGTCTCGCCC AGCTAACAAA GGAGAGTCTG CCGCGCCTGG ACAACTACCG CATTTCCATG 
CGCAACCTCA AGCGTCCTTC TATCGGCGAA CTTCAAGGAG AGGCGGTCGA CCAGAGCATC 
ACTATTCCCG AACCCGAACC GGAGGCAACT GGCGGACACA TCAAGCTAGG ATGGATCGTG 
GGCGTGCTGA TACCTTGCCT TCTCAACATC TGGGGCGTAA TGCTCTTCCT GCGCCTCAGC 
TGGGTGGTGG CCGAGTCGGG CATCCTGCAG TCCCTGATTA TAATTACGAT CTCCGCGGTG 
GTCTGCGTCA TCACGACGCT CTCGCTGTCG GCCATTAGCA CCAACGGCGA GGTCAAGGGC 
GGCGGCGTCT ACTTCATCAT ATCCCGTTCG CTGGGCCCCG AGTTTGGGGC GTCGGTGGGC 
GTGGTCTTCG CCTTCGCCAA CGCCGTCTCA GCCTCGATGA ACACCATCGG CTTCTGCGAA 
TCGCTAAATG TTTTACTCAA GAACAACGAC CTGAAGATTG TCGACAACGG GATCAATGAC 
ATCCGGATCG TGGGATCGAT AACCGTGCTG GTGCTCATCC TAATCTGCTG CGTGGGTATG 
GAGTGGGAGA CGAAGGCGCA GAACTTCCTG ATCGTCACGA TTGTCCTGGC CATCTTCAAT 
TTCCTGATCG GAGCTGCCAT TGGACCCCAG GGTAATGAGG AGCAAATCTC TAGGGGTTTC 
GTCGGCTTTT CATGGGCTAC TTTGAAGGAG AACTTCGGAT CGGACTATCG CTATGCGGAG 
GGCGTAAACC ACGACTTTTT CAGCGTGTTC GCTATCTTCT TTCCCAGCGT GACGGGCATC 
CAGGCAGGAG CAAATATCTG CGGAGACCTC AAGGATGCCG GAGCTGCTAT ACCGAAGGGC 
ACCTTCTGGT CCCTGCTCAT CTCGATGTCC TCATACGCTC TCTTTGTCCT CTTCGCCGGT 
GGCGCTGCTG TAAGAGATGC CTCCGGTATA CCAGCTGACC TCGTCAATGG CACCATTGTT 
TCCTCGGAGC TGCCGTGCAT GGCCACGGGC AACTGCACCT GGGGTCTCTT CAACTCGTAC 
GAAATGATGC AGGAGATGTC GCTGTGGGGA CCACTCATCT ACGCCGGCTG CTTTGCAGCC 
ACGTTAAGCA CGGCCCTGAC CAACTTGCTT TCCGTTCCCA GGCTGGTGCA GGCCCTGGGC 
ATTGACCAGA TCTACCCGGG TCTAATCTTC TTCTCGAAGC CGTACGGCAA GCACGGCGAA 
CCGTATCGCG GCTACGTGCT CACCTTCTTC ATCACCACCG GCTTCCTGCT GATCGGAGAG 
CTTAACCTGA TCGCTCCTCT GATCTCCACC TTCTACCTGG CCTCCTACGC CCTCATCAAC 
TTTTGCACAT TCCACGCGGC CTTCGTGAAG CCATTGGGAT GGCGACCCAC CTTTAAGTAC 
TACAACGCCT GGCTCAGCCT CTTCGGGTTC GCCATGTGCG TGGCCATCAT GTTCCTGATC 
AACTACGTGG CCGCCATCAT CACCTTTGGC ATCATCTTCG CCCTCTATCT GGTGGTCATG 
TACCGCAAGC CGGAGGCCAA CTGGGGATCC ACCACGCAGG CTCAGCAGTA CAAGGCTGCT 
CTAATGGCCG TCCACCGACT GCAGAACGTC TCGGATCACG TCAAGAACTA CCATCCCCAG 
GTGCTGGTGC TCTCCGGCGA TCCCAAGACC AGACCGCCAC TCGTGGACTT TGGCTACCTG 
CTGACCAAGA ACAACTCCCT CATGTTCGTG GCAAATATAA TACCGGTGCG GGTGGGCTAC 
AAGAACCGGC AGCACCTGGT GAAGGATGGA CAAAAGTACC TGGATGCCCG CAAGATCAAG 
GCCTTCTACA ATGTGATTGA TGGGTTCAGC CTGGAGGACG GAATAAATGC GTTGACAAAG 
TCAACGGGCT TCGGCAAAAT GTCTCCGAAC ATTGTGCTGG TGGGCTACAA GCCGGACTGG 
AATCGCTGTC GCAAGGAGGA GGTGGAGAGT TACTTTTCCA TTTTGTACAA TGCTTTTTCT 
CAGCGTATGG GCGTGGCCCT GCTCCGACTG CCCAACGGAT TGGATTTCTC TGAGCTGAGT 
TCCGAGGTCA CTCTGCCGGC CAACGGAATG GGTCACATGC ATACCGCCAA TGCGCATGGA 
TTCACCAACG AACTGATGCC GGCGGCCAAT GCAGCCTCGG AGCTGCTGCA CATTGACTCC 
AACCTGAACC TAGCCAGCAT GGACAGCCCC AACTCCTCTT TCACAATGCC CCAGCCAGCA 
CCGATGCCCA ACATGCAGCG CAACTCCCGC AGCTACAAGG TGACCAGCTC GGATGAGCCC 
GCCGTCACCT ACCACACCAA GGGCGGATCG GACATACCCC AAAACTTGCT GGACGCCATG 
ACCATATTCA CGCGCAAACA GCCCAAGGGC ACAATCGATG TCTTCTGGCT GTACGACGAC 
GGAGGTCTCA CAATTCTCCT GCCCTACATA ATCTCCATGC GCTCCCACTG GCAGAACTCC 
AAGCTAAGGG TGTTCGCAAT GTGCCACGGA AAAGACGAGG AGCAGGAAGA GAAGAGCATG 
GCTAGCTTGT TGACCAAGTT CCGCATCAAG TACTCTGAGC TCATTATGCT GAAGGGCGTC 
TCCGAGCAGC CGCGGGCGGA CACGGTGCTG AAGCACAAGC GGCTGATCGA GCCCTTCCGC 
CGCGGAGCCC GCAACGAGTT CGGCATCACG GACGATGAGC TGCAGAGCAT GTCGGAGAAG 
ACGAATCGCC AGCTGCGCAT CCATGAGCTG GTGGTCAAGC ACTCATCAAA CGCTTCCCTG 
GTGGTCATGT CCCTGCCCAT GCCGCGCAAG GAGGCCATCT CGGCACCGCT GTACATGTCA 
TGGCTGGAGA TGCTCACGTC GGACATGAAG TGTCCGGTGG CATTGGCGCG CGGCAACCAG 
ACGCCCGTGC TGACGCTCTA CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NKCC cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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