Hph cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hph
Entrez Gene ID	40633
Full Name	HIF prolyl hydroxylase
Synonyms	0303/04,31543,CG1114,CG14665,CG2676,CG31543,CG44015,CR31541,Dm.egl-9,Dmel\CG44015,Dmel_CG31543,Dmel_CR31541,PHD,anon-EST:Posey69,dHPH,dPHD,dPHD2,dmHPH,fga,hph,l(3)02255,l(3)82Fe,l(3)S030304
General protein information	Preferred Names HIF prolyl hydroxylase Names CG44015 gene product from transcript CG44015-RD CG44015-PA CG44015-PB CG44015-PC CG44015-PD HIF prolyl 4-hydroxylase HIF-1a prolyl hyroxylase Hph-PA Hph-PB Hph-PC Hph-PD fatiga lethal (3) 82Fe lethal (3) S030304 lopped prolyl-4-hydroxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82F3-82F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_141268.3	NP_649525.1	HIF prolyl hydroxylase, isoform A
NM_169037.3	NP_730908.1	HIF prolyl hydroxylase, isoform B
NM_001300242.1	NP_001287171.1	HIF prolyl hydroxylase, isoform D
NM_169036.2	NP_730906.1	HIF prolyl hydroxylase, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-190 is a novel regulator of the hypoxia response that represses the oxygen sensor Fatiga, leading to HIFalpha stabilization and enhancement of hypoxic responses.
Title: miR-190 Enhances HIF-Dependent Responses to Hypoxia in Drosophila by Inhibiting the Prolyl-4-hydroxylase Fatiga.

fgaB but not fgaA is induced in hypoxia, in a Sima-dependent manner, through a HIF-Responsive Element localized in the first intron of fgaA
Title: Oxygen sensing in Drosophila: multiple isoforms of the prolyl hydroxylase fatiga have different capacity to regulate HIFalpha/Sima.

Data show that extra tracheal sprouting during hypoxia depends on the Hypoxia-Inducible Factor (HIF)-alpha homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor.
Title: Cell autonomy of HIF effects in Drosophila: tracheal cells sense hypoxia and induce terminal branch sprouting.

Our data suggest that Hph, in addition to its function in hypoxic response, is a regulator of cellular growth and that it is a key mediator for CycD/Cdk4.
Title: Drosophila cyclin D/Cdk4 requires Hif-1 prolyl hydroxylase to drive cell growth.

The following Hph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03906	NM_169036.2 Latest version!	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03906	NM_001300242.1 Latest version!	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03907	NM_141268.3 Latest version!	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03908	NM_169037.3 Latest version!	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hph ( NM_001300242.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287171.1 Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03906
Clone ID Related Accession (Same CDS sequence)	NM_001300242.1 , NM_169036.2
Accession Version	NM_001300242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HIF prolyl hydroxylase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGG GTCGCGGAAA GGTCAGGGAT TCAGCAAGCC ATTCCGGCTC CCACTCCGCA 
TCCAAATCGG CCATGGACCC GCCGCGCTGC TCCATCTGCG GCACTCAGCA GCAACTACTG 
CGCTGCGCCA AGTGCAAGGC CGTCTACTAC TGCTCCCCCG CCCACCAGCA TCTCCATTGG 
CCCGACCACC GCACCGAGTG CCGCCTCCTG ACCCGCCAAA AGCTCAACAG CAGCAACAAC 
AATAAGCAGC AGCAGCGGCA GCAGATCCAG CAACTGCAAC AAGCTGTGGC ATCTGCCAAT 
CTGGAATGCA GCGGCGCCGG CGCCAACTGC TCCACCGCCC AGATGATGAC GCCTGCCCAC 
CAGGCGCAGA GTTGGCCCGC CGAGGTAGAC AACCTGCTGA ATCTCCTCGG GCAGCCGGGC 
AGCCAGGAGA AGGCTGCAGC TGCGGAAACG GAAACAGGTC AAAGGCAGCA GCAGCACCAG 
CATCATCACC ATAATGGCGA AAAGAGCTCC AGCTATCAAA TCGGTCTGGC GGATGCCAGC 
TTCATGGGAT CAGGAAGTGA GCGCCGCTAT GAGGATCTGT GCCGTAACAT CATCAGCGAC 
ATGAACCAGT ATGGTCTGTC CGTGGTGGAC GACTTCCTGG GGATGGAGAC GGGCCTGAAG 
ATCCTCAACG AGGTTCGAAG CATGTACAAC GCAGGAGCCT TCCAGGATGG CCAAGTGGTG 
ACCAACCAGA CGCCCGATGC ACCCGCGGTG CGCGGTGACA AGATCCGAGG CGATAAGATC 
AAGTGGGTTG GTGGCAATGA GCCGGGCTGC AGCAATGTCT GGTATCTGAC CAATCAGATT 
GACTCTGTGG TGTATCGTGT TAACACGATG AAGGATAATG GCATCTTGGG CAACTACCAC 
ATCAGGGAGC GCACGAGGGC AATGGTCGCT TGTTATCCGG GATCGGGAAC TCACTACGTC 
ATGCATGTGG ACAATCCCCA AAAGGATGGC CGCGTTATAA CGGCCATATA CTACCTGAAT 
ATCAACTGGG ATGCGCGGGA AAGTGGCGGC ATTCTGCGAA TTCGGCCAAC ACCCGGAACC 
ACAGTGGCGG ATATTGAGCC CAAGTTTGAT CGCCTGATAT TCTTCTGGTC TGACATTCGG 
AATCCCCACG AAGTGCAGCC CGCTCACCGT ACCCGCTATG CCATCACCGT CTGGTACTTC 
GATGCCAAGG AACGCGAGGA GGCCCTCATT AGGGCCAAGC TGGAAAACAG CAAGACGAAC 
AATCTGGCAG CTCAAGCCCA AGCCCAACAG GCTGAACCAG ACTCCACCAC CACACCACCC 
GCAGCACCAG CTTCATCCGC ATCCAGTCTG CCGGTTAGCA TGTCCACGGG AACGGGAGCG 
CTGAACGCCA ATGTGTCGAG TAATTCCTGC GCCACCAGCA GCGAAATATG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287171.1
CDS	529..1965
Translation	MSRGRGKVRDSASHSGSHSASKSAMDPPRCSICGTQQQLLRCAKCKAVYYCSPAHQHLHWPDHRTECRLLTRQKLNSSNNNKQQQRQQIQQLQQAVASANLECSGAGANCSTAQMMTPAHQAQSWPAEVDNLLNLLGQPGSQEKAAAAETETGQRQQQHQHHHHNGEKSSSYQIGLADASFMGSGSERRYEDLCRNIISDMNQYGLSVVDDFLGMETGLKILNEVRSMYNAGAFQDGQVVTNQTPDAPAVRGDKIRGDKIKWVGGNEPGCSNVWYLTNQIDSVVYRVNTMKDNGILGNYHIRERTRAMVACYPGSGTHYVMHVDNPQKDGRVITAIYYLNINWDARESGGILRIRPTPGTTVADIEPKFDRLIFFWSDIRNPHEVQPAHRTRYAITVWYFDAKEREEALIRAKLENSKTNNLAAQAQAQQAEPDSTTTPPAAPASSASSLPVSMSTGTGALNANVSSNSCATSSEICT

Target ORF information:

RefSeq Version	NM_001300242.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300242.1

ORF Insert Sequence:

ATGAGCAGGG GTCGCGGAAA GGTCAGGGAT TCAGCAAGCC ATTCCGGCTC CCACTCCGCA 
TCCAAATCGG CCATGGACCC GCCGCGCTGC TCCATCTGCG GCACTCAGCA GCAACTACTG 
CGCTGCGCCA AGTGCAAGGC CGTCTACTAC TGCTCCCCCG CCCACCAGCA TCTCCATTGG 
CCCGACCACC GCACCGAGTG CCGCCTCCTG ACCCGCCAAA AGCTCAACAG CAGCAACAAC 
AATAAGCAGC AGCAGCGGCA GCAGATCCAG CAACTGCAAC AAGCTGTGGC ATCTGCCAAT 
CTGGAATGCA GCGGCGCCGG CGCCAACTGC TCCACCGCCC AGATGATGAC GCCTGCCCAC 
CAGGCGCAGA GTTGGCCCGC CGAGGTAGAC AACCTGCTGA ATCTCCTCGG GCAGCCGGGC 
AGCCAGGAGA AGGCTGCAGC TGCGGAAACG GAAACAGGTC AAAGGCAGCA GCAGCACCAG 
CATCATCACC ATAATGGCGA AAAGAGCTCC AGCTATCAAA TCGGTCTGGC GGATGCCAGC 
TTCATGGGAT CAGGAAGTGA GCGCCGCTAT GAGGATCTGT GCCGTAACAT CATCAGCGAC 
ATGAACCAGT ATGGTCTGTC CGTGGTGGAC GACTTCCTGG GGATGGAGAC GGGCCTGAAG 
ATCCTCAACG AGGTTCGAAG CATGTACAAC GCAGGAGCCT TCCAGGATGG CCAAGTGGTG 
ACCAACCAGA CGCCCGATGC ACCCGCGGTG CGCGGTGACA AGATCCGAGG CGATAAGATC 
AAGTGGGTTG GTGGCAATGA GCCGGGCTGC AGCAATGTCT GGTATCTGAC CAATCAGATT 
GACTCTGTGG TGTATCGTGT TAACACGATG AAGGATAATG GCATCTTGGG CAACTACCAC 
ATCAGGGAGC GCACGAGGGC AATGGTCGCT TGTTATCCGG GATCGGGAAC TCACTACGTC 
ATGCATGTGG ACAATCCCCA AAAGGATGGC CGCGTTATAA CGGCCATATA CTACCTGAAT 
ATCAACTGGG ATGCGCGGGA AAGTGGCGGC ATTCTGCGAA TTCGGCCAAC ACCCGGAACC 
ACAGTGGCGG ATATTGAGCC CAAGTTTGAT CGCCTGATAT TCTTCTGGTC TGACATTCGG 
AATCCCCACG AAGTGCAGCC CGCTCACCGT ACCCGCTATG CCATCACCGT CTGGTACTTC 
GATGCCAAGG AACGCGAGGA GGCCCTCATT AGGGCCAAGC TGGAAAACAG CAAGACGAAC 
AATCTGGCAG CTCAAGCCCA AGCCCAACAG GCTGAACCAG ACTCCACCAC CACACCACCC 
GCAGCACCAG CTTCATCCGC ATCCAGTCTG CCGGTTAGCA TGTCCACGGG AACGGGAGCG 
CTGAACGCCA ATGTGTCGAG TAATTCCTGC GCCACCAGCA GCGAAATATG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hph ( NM_169036.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730906.1 Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03906
Clone ID Related Accession (Same CDS sequence)	NM_001300242.1 , NM_169036.2
Accession Version	NM_169036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HIF prolyl hydroxylase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169036.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGGG GTCGCGGAAA GGTCAGGGAT TCAGCAAGCC ATTCCGGCTC CCACTCCGCA 
TCCAAATCGG CCATGGACCC GCCGCGCTGC TCCATCTGCG GCACTCAGCA GCAACTACTG 
CGCTGCGCCA AGTGCAAGGC CGTCTACTAC TGCTCCCCCG CCCACCAGCA TCTCCATTGG 
CCCGACCACC GCACCGAGTG CCGCCTCCTG ACCCGCCAAA AGCTCAACAG CAGCAACAAC 
AATAAGCAGC AGCAGCGGCA GCAGATCCAG CAACTGCAAC AAGCTGTGGC ATCTGCCAAT 
CTGGAATGCA GCGGCGCCGG CGCCAACTGC TCCACCGCCC AGATGATGAC GCCTGCCCAC 
CAGGCGCAGA GTTGGCCCGC CGAGGTAGAC AACCTGCTGA ATCTCCTCGG GCAGCCGGGC 
AGCCAGGAGA AGGCTGCAGC TGCGGAAACG GAAACAGGTC AAAGGCAGCA GCAGCACCAG 
CATCATCACC ATAATGGCGA AAAGAGCTCC AGCTATCAAA TCGGTCTGGC GGATGCCAGC 
TTCATGGGAT CAGGAAGTGA GCGCCGCTAT GAGGATCTGT GCCGTAACAT CATCAGCGAC 
ATGAACCAGT ATGGTCTGTC CGTGGTGGAC GACTTCCTGG GGATGGAGAC GGGCCTGAAG 
ATCCTCAACG AGGTTCGAAG CATGTACAAC GCAGGAGCCT TCCAGGATGG CCAAGTGGTG 
ACCAACCAGA CGCCCGATGC ACCCGCGGTG CGCGGTGACA AGATCCGAGG CGATAAGATC 
AAGTGGGTTG GTGGCAATGA GCCGGGCTGC AGCAATGTCT GGTATCTGAC CAATCAGATT 
GACTCTGTGG TGTATCGTGT TAACACGATG AAGGATAATG GCATCTTGGG CAACTACCAC 
ATCAGGGAGC GCACGAGGGC AATGGTCGCT TGTTATCCGG GATCGGGAAC TCACTACGTC 
ATGCATGTGG ACAATCCCCA AAAGGATGGC CGCGTTATAA CGGCCATATA CTACCTGAAT 
ATCAACTGGG ATGCGCGGGA AAGTGGCGGC ATTCTGCGAA TTCGGCCAAC ACCCGGAACC 
ACAGTGGCGG ATATTGAGCC CAAGTTTGAT CGCCTGATAT TCTTCTGGTC TGACATTCGG 
AATCCCCACG AAGTGCAGCC CGCTCACCGT ACCCGCTATG CCATCACCGT CTGGTACTTC 
GATGCCAAGG AACGCGAGGA GGCCCTCATT AGGGCCAAGC TGGAAAACAG CAAGACGAAC 
AATCTGGCAG CTCAAGCCCA AGCCCAACAG GCTGAACCAG ACTCCACCAC CACACCACCC 
GCAGCACCAG CTTCATCCGC ATCCAGTCTG CCGGTTAGCA TGTCCACGGG AACGGGAGCG 
CTGAACGCCA ATGTGTCGAG TAATTCCTGC GCCACCAGCA GCGAAATATG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730906.1
CDS	529..1965
Translation	MSRGRGKVRDSASHSGSHSASKSAMDPPRCSICGTQQQLLRCAKCKAVYYCSPAHQHLHWPDHRTECRLLTRQKLNSSNNNKQQQRQQIQQLQQAVASANLECSGAGANCSTAQMMTPAHQAQSWPAEVDNLLNLLGQPGSQEKAAAAETETGQRQQQHQHHHHNGEKSSSYQIGLADASFMGSGSERRYEDLCRNIISDMNQYGLSVVDDFLGMETGLKILNEVRSMYNAGAFQDGQVVTNQTPDAPAVRGDKIRGDKIKWVGGNEPGCSNVWYLTNQIDSVVYRVNTMKDNGILGNYHIRERTRAMVACYPGSGTHYVMHVDNPQKDGRVITAIYYLNINWDARESGGILRIRPTPGTTVADIEPKFDRLIFFWSDIRNPHEVQPAHRTRYAITVWYFDAKEREEALIRAKLENSKTNNLAAQAQAQQAEPDSTTTPPAAPASSASSLPVSMSTGTGALNANVSSNSCATSSEICT

Target ORF information:

RefSeq Version	NM_169036.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169036.2

ORF Insert Sequence:

ATGAGCAGGG GTCGCGGAAA GGTCAGGGAT TCAGCAAGCC ATTCCGGCTC CCACTCCGCA 
TCCAAATCGG CCATGGACCC GCCGCGCTGC TCCATCTGCG GCACTCAGCA GCAACTACTG 
CGCTGCGCCA AGTGCAAGGC CGTCTACTAC TGCTCCCCCG CCCACCAGCA TCTCCATTGG 
CCCGACCACC GCACCGAGTG CCGCCTCCTG ACCCGCCAAA AGCTCAACAG CAGCAACAAC 
AATAAGCAGC AGCAGCGGCA GCAGATCCAG CAACTGCAAC AAGCTGTGGC ATCTGCCAAT 
CTGGAATGCA GCGGCGCCGG CGCCAACTGC TCCACCGCCC AGATGATGAC GCCTGCCCAC 
CAGGCGCAGA GTTGGCCCGC CGAGGTAGAC AACCTGCTGA ATCTCCTCGG GCAGCCGGGC 
AGCCAGGAGA AGGCTGCAGC TGCGGAAACG GAAACAGGTC AAAGGCAGCA GCAGCACCAG 
CATCATCACC ATAATGGCGA AAAGAGCTCC AGCTATCAAA TCGGTCTGGC GGATGCCAGC 
TTCATGGGAT CAGGAAGTGA GCGCCGCTAT GAGGATCTGT GCCGTAACAT CATCAGCGAC 
ATGAACCAGT ATGGTCTGTC CGTGGTGGAC GACTTCCTGG GGATGGAGAC GGGCCTGAAG 
ATCCTCAACG AGGTTCGAAG CATGTACAAC GCAGGAGCCT TCCAGGATGG CCAAGTGGTG 
ACCAACCAGA CGCCCGATGC ACCCGCGGTG CGCGGTGACA AGATCCGAGG CGATAAGATC 
AAGTGGGTTG GTGGCAATGA GCCGGGCTGC AGCAATGTCT GGTATCTGAC CAATCAGATT 
GACTCTGTGG TGTATCGTGT TAACACGATG AAGGATAATG GCATCTTGGG CAACTACCAC 
ATCAGGGAGC GCACGAGGGC AATGGTCGCT TGTTATCCGG GATCGGGAAC TCACTACGTC 
ATGCATGTGG ACAATCCCCA AAAGGATGGC CGCGTTATAA CGGCCATATA CTACCTGAAT 
ATCAACTGGG ATGCGCGGGA AAGTGGCGGC ATTCTGCGAA TTCGGCCAAC ACCCGGAACC 
ACAGTGGCGG ATATTGAGCC CAAGTTTGAT CGCCTGATAT TCTTCTGGTC TGACATTCGG 
AATCCCCACG AAGTGCAGCC CGCTCACCGT ACCCGCTATG CCATCACCGT CTGGTACTTC 
GATGCCAAGG AACGCGAGGA GGCCCTCATT AGGGCCAAGC TGGAAAACAG CAAGACGAAC 
AATCTGGCAG CTCAAGCCCA AGCCCAACAG GCTGAACCAG ACTCCACCAC CACACCACCC 
GCAGCACCAG CTTCATCCGC ATCCAGTCTG CCGGTTAGCA TGTCCACGGG AACGGGAGCG 
CTGAACGCCA ATGTGTCGAG TAATTCCTGC GCCACCAGCA GCGAAATATG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hph ( NM_141268.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649525.1 Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03907
Clone ID Related Accession (Same CDS sequence)	NM_141268.3
Accession Version	NM_141268.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HIF prolyl hydroxylase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141268.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAACCT CCACGACCAC GGACTACAAA AACTTTTTTA AACACTCGGC ACATCCTGCC 
AATGCTGAGC AGTACTTTCG GGAACTTTTG GACAAGCGTG AGCGCCGCTA TGAGGATCTG 
TGCCGTAACA TCATCAGCGA CATGAACCAG TATGGTCTGT CCGTGGTGGA CGACTTCCTG 
GGGATGGAGA CGGGCCTGAA GATCCTCAAC GAGGTTCGAA GCATGTACAA CGCAGGAGCC 
TTCCAGGATG GCCAAGTGGT GACCAACCAG ACGCCCGATG CACCCGCGGT GCGCGGTGAC 
AAGATCCGAG GCGATAAGAT CAAGTGGGTT GGTGGCAATG AGCCGGGCTG CAGCAATGTC 
TGGTATCTGA CCAATCAGAT TGACTCTGTG GTGTATCGTG TTAACACGAT GAAGGATAAT 
GGCATCTTGG GCAACTACCA CATCAGGGAG CGCACGAGGG CAATGGTCGC TTGTTATCCG 
GGATCGGGAA CTCACTACGT CATGCATGTG GACAATCCCC AAAAGGATGG CCGCGTTATA 
ACGGCCATAT ACTACCTGAA TATCAACTGG GATGCGCGGG AAAGTGGCGG CATTCTGCGA 
ATTCGGCCAA CACCCGGAAC CACAGTGGCG GATATTGAGC CCAAGTTTGA TCGCCTGATA 
TTCTTCTGGT CTGACATTCG GAATCCCCAC GAAGTGCAGC CCGCTCACCG TACCCGCTAT 
GCCATCACCG TCTGGTACTT CGATGCCAAG GAACGCGAGG AGGCCCTCAT TAGGGCCAAG 
CTGGAAAACA GCAAGACGAA CAATCTGGCA GCTCAAGCCC AAGCCCAACA GGCTGAACCA 
GACTCCACCA CCACACCACC CGCAGCACCA GCTTCATCCG CATCCAGTCT GCCGGTTAGC 
ATGTCCACGG GAACGGGAGC GCTGAACGCC AATGTGTCGA GTAATTCCTG CGCCACCAGC 
AGCGAAATAT GCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649525.1
CDS	602..1579
Translation	MITSTTTDYKNFFKHSAHPANAEQYFRELLDKRERRYEDLCRNIISDMNQYGLSVVDDFLGMETGLKILNEVRSMYNAGAFQDGQVVTNQTPDAPAVRGDKIRGDKIKWVGGNEPGCSNVWYLTNQIDSVVYRVNTMKDNGILGNYHIRERTRAMVACYPGSGTHYVMHVDNPQKDGRVITAIYYLNINWDARESGGILRIRPTPGTTVADIEPKFDRLIFFWSDIRNPHEVQPAHRTRYAITVWYFDAKEREEALIRAKLENSKTNNLAAQAQAQQAEPDSTTTPPAAPASSASSLPVSMSTGTGALNANVSSNSCATSSEICT

Target ORF information:

RefSeq Version	NM_141268.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141268.3

ORF Insert Sequence:

ATGATAACCT CCACGACCAC GGACTACAAA AACTTTTTTA AACACTCGGC ACATCCTGCC 
AATGCTGAGC AGTACTTTCG GGAACTTTTG GACAAGCGTG AGCGCCGCTA TGAGGATCTG 
TGCCGTAACA TCATCAGCGA CATGAACCAG TATGGTCTGT CCGTGGTGGA CGACTTCCTG 
GGGATGGAGA CGGGCCTGAA GATCCTCAAC GAGGTTCGAA GCATGTACAA CGCAGGAGCC 
TTCCAGGATG GCCAAGTGGT GACCAACCAG ACGCCCGATG CACCCGCGGT GCGCGGTGAC 
AAGATCCGAG GCGATAAGAT CAAGTGGGTT GGTGGCAATG AGCCGGGCTG CAGCAATGTC 
TGGTATCTGA CCAATCAGAT TGACTCTGTG GTGTATCGTG TTAACACGAT GAAGGATAAT 
GGCATCTTGG GCAACTACCA CATCAGGGAG CGCACGAGGG CAATGGTCGC TTGTTATCCG 
GGATCGGGAA CTCACTACGT CATGCATGTG GACAATCCCC AAAAGGATGG CCGCGTTATA 
ACGGCCATAT ACTACCTGAA TATCAACTGG GATGCGCGGG AAAGTGGCGG CATTCTGCGA 
ATTCGGCCAA CACCCGGAAC CACAGTGGCG GATATTGAGC CCAAGTTTGA TCGCCTGATA 
TTCTTCTGGT CTGACATTCG GAATCCCCAC GAAGTGCAGC CCGCTCACCG TACCCGCTAT 
GCCATCACCG TCTGGTACTT CGATGCCAAG GAACGCGAGG AGGCCCTCAT TAGGGCCAAG 
CTGGAAAACA GCAAGACGAA CAATCTGGCA GCTCAAGCCC AAGCCCAACA GGCTGAACCA 
GACTCCACCA CCACACCACC CGCAGCACCA GCTTCATCCG CATCCAGTCT GCCGGTTAGC 
ATGTCCACGG GAACGGGAGC GCTGAACGCC AATGTGTCGA GTAATTCCTG CGCCACCAGC 
AGCGAAATAT GCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hph ( NM_169037.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730908.1 Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant B, mRNA.

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CloneID	OFa03908
Clone ID Related Accession (Same CDS sequence)	NM_169037.3
Accession Version	NM_169037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HIF prolyl hydroxylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169037.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACCAGT ATGGTCTGTC CGTGGTGGAC GACTTCCTGG GGATGGAGAC GGGCCTGAAG 
ATCCTCAACG AGGTTCGAAG CATGTACAAC GCAGGAGCCT TCCAGGATGG CCAAGTGGTG 
ACCAACCAGA CGCCCGATGC ACCCGCGGTG CGCGGTGACA AGATCCGAGG CGATAAGATC 
AAGTGGGTTG GTGGCAATGA GCCGGGCTGC AGCAATGTCT GGTATCTGAC CAATCAGATT 
GACTCTGTGG TGTATCGTGT TAACACGATG AAGGATAATG GCATCTTGGG CAACTACCAC 
ATCAGGGAGC GCACGAGGGC AATGGTCGCT TGTTATCCGG GATCGGGAAC TCACTACGTC 
ATGCATGTGG ACAATCCCCA AAAGGATGGC CGCGTTATAA CGGCCATATA CTACCTGAAT 
ATCAACTGGG ATGCGCGGGA AAGTGGCGGC ATTCTGCGAA TTCGGCCAAC ACCCGGAACC 
ACAGTGGCGG ATATTGAGCC CAAGTTTGAT CGCCTGATAT TCTTCTGGTC TGACATTCGG 
AATCCCCACG AAGTGCAGCC CGCTCACCGT ACCCGCTATG CCATCACCGT CTGGTACTTC 
GATGCCAAGG AACGCGAGGA GGCCCTCATT AGGGCCAAGC TGGAAAACAG CAAGACGAAC 
AATCTGGCAG CTCAAGCCCA AGCCCAACAG GCTGAACCAG ACTCCACCAC CACACCACCC 
GCAGCACCAG CTTCATCCGC ATCCAGTCTG CCGGTTAGCA TGTCCACGGG AACGGGAGCG 
CTGAACGCCA ATGTGTCGAG TAATTCCTGC GCCACCAGCA GCGAAATATG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730908.1
CDS	308..1144
Translation	MNQYGLSVVDDFLGMETGLKILNEVRSMYNAGAFQDGQVVTNQTPDAPAVRGDKIRGDKIKWVGGNEPGCSNVWYLTNQIDSVVYRVNTMKDNGILGNYHIRERTRAMVACYPGSGTHYVMHVDNPQKDGRVITAIYYLNINWDARESGGILRIRPTPGTTVADIEPKFDRLIFFWSDIRNPHEVQPAHRTRYAITVWYFDAKEREEALIRAKLENSKTNNLAAQAQAQQAEPDSTTTPPAAPASSASSLPVSMSTGTGALNANVSSNSCATSSEICT

Target ORF information:

RefSeq Version	NM_169037.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HIF prolyl hydroxylase (Hph), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169037.3

ORF Insert Sequence:

ATGAACCAGT ATGGTCTGTC CGTGGTGGAC GACTTCCTGG GGATGGAGAC GGGCCTGAAG 
ATCCTCAACG AGGTTCGAAG CATGTACAAC GCAGGAGCCT TCCAGGATGG CCAAGTGGTG 
ACCAACCAGA CGCCCGATGC ACCCGCGGTG CGCGGTGACA AGATCCGAGG CGATAAGATC 
AAGTGGGTTG GTGGCAATGA GCCGGGCTGC AGCAATGTCT GGTATCTGAC CAATCAGATT 
GACTCTGTGG TGTATCGTGT TAACACGATG AAGGATAATG GCATCTTGGG CAACTACCAC 
ATCAGGGAGC GCACGAGGGC AATGGTCGCT TGTTATCCGG GATCGGGAAC TCACTACGTC 
ATGCATGTGG ACAATCCCCA AAAGGATGGC CGCGTTATAA CGGCCATATA CTACCTGAAT 
ATCAACTGGG ATGCGCGGGA AAGTGGCGGC ATTCTGCGAA TTCGGCCAAC ACCCGGAACC 
ACAGTGGCGG ATATTGAGCC CAAGTTTGAT CGCCTGATAT TCTTCTGGTC TGACATTCGG 
AATCCCCACG AAGTGCAGCC CGCTCACCGT ACCCGCTATG CCATCACCGT CTGGTACTTC 
GATGCCAAGG AACGCGAGGA GGCCCTCATT AGGGCCAAGC TGGAAAACAG CAAGACGAAC 
AATCTGGCAG CTCAAGCCCA AGCCCAACAG GCTGAACCAG ACTCCACCAC CACACCACCC 
GCAGCACCAG CTTCATCCGC ATCCAGTCTG CCGGTTAGCA TGTCCACGGG AACGGGAGCG 
CTGAACGCCA ATGTGTCGAG TAATTCCTGC GCCACCAGCA GCGAAATATG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hph cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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