srl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	srl
Entrez Gene ID	40562
Full Name	spargel
Synonyms	CG 9809,CG9809,Dmel\CG9809,PGC-1,PGC-1-alpha,Spargel,Srl,cg9809,dPCG1alpha,dPGC,dPGC-1,dPGC1
General protein information	Preferred Names spargel Names CG9809 gene product from transcript CG9809-RB CG9809-PB CG9809-PD CG9809-PE peroxisome proliferator-activated receptor gamma coactivator 1 srl-PB srl-PD srl-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82B1-82B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_168997.5	NP_730836.3	spargel, isoform D
NM_168996.5	NP_730835.2	spargel, isoform B
NM_141212.4	NP_649469.2	spargel, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic or pharmacological activation of PGC-1a may be of therapeutic value to Parkinson's disease.
Title: Genetic or pharmacological activation of the Drosophila PGC-1? ortholog spargel rescues the disease phenotypes of genetic models of Parkinson's disease.

The reduced lifespan and climbing ability associated with a tissue specific expression of srl in DA neurons provides a new model of PD in D. melanogaster.
Title: spargel, the PGC-1? homologue, in models of Parkinson disease in Drosophila melanogaster.

Our data suggest INDY may be a physiological regulator that modulates intermediary metabolism in response to changes in nutrient availability and organismal needs by modulating dPGC-1.
Title: Increased mitochondrial biogenesis preserves intestinal stem cell homeostasis and contributes to longevity in Indy mutant flies.

This led us to conclude that Spargel should be incorporated as a new member of this growth-signaling pathway
Title: Spargel/dPGC-1 is a new downstream effector in the insulin-TOR signaling pathway in Drosophila.

dPGC-1 (spargel) can slow aging both at the level of cellular changes in an individual tissue and also at the organismal level by extending life span.
Title: Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog.

The following srl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03828	NM_141212.4 Latest version!	Drosophila melanogaster spargel (srl), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03828	NM_168996.5 Latest version!	Drosophila melanogaster spargel (srl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa03829	NM_168997.5 Latest version!	Drosophila melanogaster spargel (srl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

srl ( NM_168996.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730835.2 Drosophila melanogaster spargel (srl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03828
Clone ID Related Accession (Same CDS sequence)	NM_168996.5 , NM_141212.4
Accession Version	NM_168996.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spargel, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_168996.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCAC GAATGCTAAA TGTGTTCCAA GGAGATACCT TTGAGGCAAA TTATACCAAA 
TACGAGTCTG AGCCGATATT CTGGTCATCT AATGATGAAC TGCAACTGAC AGATACACTG 
AGCGGCATCC TACCCATCGC GCCCACAGAC GAATCACTGC TCAACCATAA CCGGGAGGAA 
GATCTGCAAA ACAGAAAGAA TCTGCTGATT TACAACGACT TTCTGAAACA GGAGCTCAGC 
AACCATGATG CCAATGAGCT AAATGCCCAG GTCTATCGGT CCATCGCCGA CAGCGGGGGC 
GAGATTGCCT ATGAGCAGCA GCGCCAGATC AGCGCCATTG TGGAGTACGA CAAAGAAGAT 
ATTAAAATCG ACGGCCTTAC GGAGGAGGAG GACATACAAA GCCAGTCCAC ATTTCGCACC 
CGCACAGACA GCTCTTCGAT TGGTGACTAT GAATCGCACT CTAGCGACTA TGATGATGAA 
TTTGACATTG TCAACGAAGA AAAAAGTCCA AGGCTGCAGG TTGTCCATAA CAATATCGGC 
AACTTGCAGA GCTCCTGTGA GCGCAAGGGA CGGACGCTCA GTACTAACAC TATTATCTCC 
GAAGTAGACG CATTGGGGCT AAGTATTGAT GTTAATAACT TTGACCTAGC TGATTTTATT 
ACCAAAGACG ATTTTGCTGA GAATTTAAAC GCCTGTCGCA AAAAGGAATC TCTGCAAGCA 
CAGGCGCAAA TTAAATCTAA TACTTTAGCA GATGTTCCCA TACTTCTTAA TCCACCGGTT 
GCCACAATTG TGGCAAAACC CGGGGTCCCA TCCTCTAAAC CTGGCGATTC TGATTATGAC 
TCTGACTCGA TTATCGACGT TGAAACTGTC GATGTACTCG ATATGAATAT GGCCAATATC 
TGGTCAATAG ATAAGCCGGC AAAAATGGGC CAAACTCCCG ATGAATTACG CTATGTGGAC 
AATGTAAAGG CCGATCCCTC TTGGAGTCCG AGATCCGCAA AAAAGACCAC ACCTACTATC 
AAAGAGAATA AGCCGGAACA ACTGGGGAAC CATCAGCTCG CGGTCCCAGC ATCAAACAAA 
CCGAAGCATA AGAATATTTG CCTGGTGCCT AATAAAAAAC AGTGCGATTT TCTTAAACGT 
AAGGTTGGTA GTCCCTCCTT GTCCAAGCTA ACTAAGGCGA CAACAAAAAA AGCGACTGAG 
TCTATGAGCA ACAAGCAGCC GTCAGTAAAA AATACTCAAT GCACGGGTCT CGGATTGGGT 
GGAAAAAACC TTTCGCTTCT GAAGCAGGAG AGGGACGCCG CATTATCGGT GTGTGCTGCA 
AAACCTATAG CACCTCCAAC CCTTTCGACA ACAACCCCGA CTTTGACACC GCCAGCAAAG 
CGAAAGCTAA ATCTAGAGGA ATATAAACAG CGTCGTTGTG GCGGTGTGGG GGCTGTGTAT 
CCTAAACCAA CTCCGCCCAA GCAGGCGAAA ATAGAGGTTG CCGCCAAACG CATTGCTCCT 
ACCCCGGTTT CAAAACCAAC ACCTTCACCA AAAAAAGCGG AAATAGTCAA TATTGTAAAC 
AGCCTTAAAT GTCCTCGCAC AGAACAATCT TCTTTGCCCA TGGATCCCAT TACCATGGCC 
AAGAACAAGG TGCTGCGTAT GCTAGAGATG AAACGTGCCC AACAGCTAAA AATAATCGAT 
TCCCGCGTAT CAGCCAAGGT GCCACGCGTA ACCAAGCTGC CGCCACTTAA GGATATTGTA 
AAGGACACCT ACTGCATGGA AACTGAGGAT CCTGGCACAG AGATAGCGCC ACTCAGCAAC 
AAACTCCACC CGGACTATGA GGAGATTATA ATAGTTTCAG CTAGTTGCAA TACAGACATA 
ACAATACCTC CCAATCAATT AAGCAAGGCC TCCCCTCGCT CTCTCTTAAA GTCATCAGTT 
TTGCTGTACA ACATCTCAAA CGGCCAGGAT GCCAATAAGA ACATGAGCAA CTCCCTAATA 
GCCAGCATCC AAAGCGAGGT CGCCAGGCAA ACGAGTAACA CTACTCTCTC GACGATACAG 
TCCAACGCAA CAAAAGTTAT GTCTTCCGCT GATAAGAATT GTCAGCATGG CGAGGACATG 
GTCATCATGC ACTTACCCAA GGATCGGGTG CGAAAAACTC TTGTGAGCAT TGCAACCCAA 
ACTGATCTGC AACCCGAATT TCCCCTATTG ACGCTGCCCC CAAAGCGACA GTCTCGAGAG 
CGTACGCGTC GAAATTATCG CAGGCGACGG ACACAAGGCT CGGGCTCAAA TATGTCCACT 
TCTTCGAGTG ACTTTTCCTC CGACTGCAGC AGCTTGGTGT CACACAGATC TCGCTCACAA 
TACGATTCGA TTGATCAGCT GCGTAACTTA GATGTAGCAG CCACGTGTGG GGGCAGTATT 
GGAAACGGCG GTTATTCTTC TCGTAGCACT CAACGCCATC GCAGCTCGGT GAGTTCTTCA 
TCTTACTCAG AGAATGGACA GTACAGGCGC AGGCAGCGCC GCACGAGTTA CAACAAGCGT 
AGATCTAGAA ACCAAAAGCG GGGCAGTACT TCCTCGTCAT GCTCGGGCTC CGAAAAAAGC 
GATCGCGAAC GTAGCCGCAG CCCACACAGA AAATTGCGCC ACTCGCGGTC CCGGTCGCGC 
TCAAAATCTG ATACACGCTA CCCTAACAAC AACTCCTCCT CCAATAACAA CAATCGGCGA 
GGATTTTTTG ATCGTAACGT GTCGCAACCG GCCGTTGAAG AGCGGCGAAT CGTCTACGTC 
GGTCGCATTG AGCAAGAAAC CACCAAGGAA ATTCTGCGTC GTAAATTTCT GCCCTATGGA 
AGCATTAAAC AAATAACAAT ACACTACAAG GAAAATGGAA TGAAGTATGG GTTCGTTACG 
TACGAAAGAG CGCAGGACGC ATTCACGGCC ATTGACACGA GTCATAGAGA CTCGCAAATA 
AGCATGTATG ACATAAGTTT TGGAGGAAGA CGTGCCTTCT GTCGTTCATC TTACGCTGAC 
TTGGATAATG CTGGCATTAA TAATTACAAC TCGTATGTGT TTCCCAAGGA AGCACCAGCA 
CCGAATGTAG TCGAGGACTC TTTCGAAGCT TTGTTGCTTC AGGTAAAAGC AAAGTTAAAC 
GCGGGCAAGT CACCGGTAGG TTCATCTACG CTGGAGGCAC CATCAGCTTC CGGTACTGTA 
CTTCAGGGCC ACAGTCAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730835.2
CDS	383..3586
Translation	MDSRMLNVFQGDTFEANYTKYESEPIFWSSNDELQLTDTLSGILPIAPTDESLLNHNREEDLQNRKNLLIYNDFLKQELSNHDANELNAQVYRSIADSGGEIAYEQQRQISAIVEYDKEDIKIDGLTEEEDIQSQSTFRTRTDSSSIGDYESHSSDYDDEFDIVNEEKSPRLQVVHNNIGNLQSSCERKGRTLSTNTIISEVDALGLSIDVNNFDLADFITKDDFAENLNACRKKESLQAQAQIKSNTLADVPILLNPPVATIVAKPGVPSSKPGDSDYDSDSIIDVETVDVLDMNMANIWSIDKPAKMGQTPDELRYVDNVKADPSWSPRSAKKTTPTIKENKPEQLGNHQLAVPASNKPKHKNICLVPNKKQCDFLKRKVGSPSLSKLTKATTKKATESMSNKQPSVKNTQCTGLGLGGKNLSLLKQERDAALSVCAAKPIAPPTLSTTTPTLTPPAKRKLNLEEYKQRRCGGVGAVYPKPTPPKQAKIEVAAKRIAPTPVSKPTPSPKKAEIVNIVNSLKCPRTEQSSLPMDPITMAKNKVLRMLEMKRAQQLKIIDSRVSAKVPRVTKLPPLKDIVKDTYCMETEDPGTEIAPLSNKLHPDYEEIIIVSASCNTDITIPPNQLSKASPRSLLKSSVLLYNISNGQDANKNMSNSLIASIQSEVARQTSNTTLSTIQSNATKVMSSADKNCQHGEDMVIMHLPKDRVRKTLVSIATQTDLQPEFPLLTLPPKRQSRERTRRNYRRRRTQGSGSNMSTSSSDFSSDCSSLVSHRSRSQYDSIDQLRNLDVAATCGGSIGNGGYSSRSTQRHRSSVSSSSYSENGQYRRRQRRTSYNKRRSRNQKRGSTSSSCSGSEKSDRERSRSPHRKLRHSRSRSRSKSDTRYPNNNSSSNNNNRRGFFDRNVSQPAVEERRIVYVGRIEQETTKEILRRKFLPYGSIKQITIHYKENGMKYGFVTYERAQDAFTAIDTSHRDSQISMYDISFGGRRAFCRSSYADLDNAGINNYNSYVFPKEAPAPNVVEDSFEALLLQVKAKLNAGKSPVGSSTLEAPSASGTVLQGHSQM

Target ORF information:

RefSeq Version	NM_168996.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spargel (srl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168996.5

ORF Insert Sequence:

ATGGATTCAC GAATGCTAAA TGTGTTCCAA GGAGATACCT TTGAGGCAAA TTATACCAAA 
TACGAGTCTG AGCCGATATT CTGGTCATCT AATGATGAAC TGCAACTGAC AGATACACTG 
AGCGGCATCC TACCCATCGC GCCCACAGAC GAATCACTGC TCAACCATAA CCGGGAGGAA 
GATCTGCAAA ACAGAAAGAA TCTGCTGATT TACAACGACT TTCTGAAACA GGAGCTCAGC 
AACCATGATG CCAATGAGCT AAATGCCCAG GTCTATCGGT CCATCGCCGA CAGCGGGGGC 
GAGATTGCCT ATGAGCAGCA GCGCCAGATC AGCGCCATTG TGGAGTACGA CAAAGAAGAT 
ATTAAAATCG ACGGCCTTAC GGAGGAGGAG GACATACAAA GCCAGTCCAC ATTTCGCACC 
CGCACAGACA GCTCTTCGAT TGGTGACTAT GAATCGCACT CTAGCGACTA TGATGATGAA 
TTTGACATTG TCAACGAAGA AAAAAGTCCA AGGCTGCAGG TTGTCCATAA CAATATCGGC 
AACTTGCAGA GCTCCTGTGA GCGCAAGGGA CGGACGCTCA GTACTAACAC TATTATCTCC 
GAAGTAGACG CATTGGGGCT AAGTATTGAT GTTAATAACT TTGACCTAGC TGATTTTATT 
ACCAAAGACG ATTTTGCTGA GAATTTAAAC GCCTGTCGCA AAAAGGAATC TCTGCAAGCA 
CAGGCGCAAA TTAAATCTAA TACTTTAGCA GATGTTCCCA TACTTCTTAA TCCACCGGTT 
GCCACAATTG TGGCAAAACC CGGGGTCCCA TCCTCTAAAC CTGGCGATTC TGATTATGAC 
TCTGACTCGA TTATCGACGT TGAAACTGTC GATGTACTCG ATATGAATAT GGCCAATATC 
TGGTCAATAG ATAAGCCGGC AAAAATGGGC CAAACTCCCG ATGAATTACG CTATGTGGAC 
AATGTAAAGG CCGATCCCTC TTGGAGTCCG AGATCCGCAA AAAAGACCAC ACCTACTATC 
AAAGAGAATA AGCCGGAACA ACTGGGGAAC CATCAGCTCG CGGTCCCAGC ATCAAACAAA 
CCGAAGCATA AGAATATTTG CCTGGTGCCT AATAAAAAAC AGTGCGATTT TCTTAAACGT 
AAGGTTGGTA GTCCCTCCTT GTCCAAGCTA ACTAAGGCGA CAACAAAAAA AGCGACTGAG 
TCTATGAGCA ACAAGCAGCC GTCAGTAAAA AATACTCAAT GCACGGGTCT CGGATTGGGT 
GGAAAAAACC TTTCGCTTCT GAAGCAGGAG AGGGACGCCG CATTATCGGT GTGTGCTGCA 
AAACCTATAG CACCTCCAAC CCTTTCGACA ACAACCCCGA CTTTGACACC GCCAGCAAAG 
CGAAAGCTAA ATCTAGAGGA ATATAAACAG CGTCGTTGTG GCGGTGTGGG GGCTGTGTAT 
CCTAAACCAA CTCCGCCCAA GCAGGCGAAA ATAGAGGTTG CCGCCAAACG CATTGCTCCT 
ACCCCGGTTT CAAAACCAAC ACCTTCACCA AAAAAAGCGG AAATAGTCAA TATTGTAAAC 
AGCCTTAAAT GTCCTCGCAC AGAACAATCT TCTTTGCCCA TGGATCCCAT TACCATGGCC 
AAGAACAAGG TGCTGCGTAT GCTAGAGATG AAACGTGCCC AACAGCTAAA AATAATCGAT 
TCCCGCGTAT CAGCCAAGGT GCCACGCGTA ACCAAGCTGC CGCCACTTAA GGATATTGTA 
AAGGACACCT ACTGCATGGA AACTGAGGAT CCTGGCACAG AGATAGCGCC ACTCAGCAAC 
AAACTCCACC CGGACTATGA GGAGATTATA ATAGTTTCAG CTAGTTGCAA TACAGACATA 
ACAATACCTC CCAATCAATT AAGCAAGGCC TCCCCTCGCT CTCTCTTAAA GTCATCAGTT 
TTGCTGTACA ACATCTCAAA CGGCCAGGAT GCCAATAAGA ACATGAGCAA CTCCCTAATA 
GCCAGCATCC AAAGCGAGGT CGCCAGGCAA ACGAGTAACA CTACTCTCTC GACGATACAG 
TCCAACGCAA CAAAAGTTAT GTCTTCCGCT GATAAGAATT GTCAGCATGG CGAGGACATG 
GTCATCATGC ACTTACCCAA GGATCGGGTG CGAAAAACTC TTGTGAGCAT TGCAACCCAA 
ACTGATCTGC AACCCGAATT TCCCCTATTG ACGCTGCCCC CAAAGCGACA GTCTCGAGAG 
CGTACGCGTC GAAATTATCG CAGGCGACGG ACACAAGGCT CGGGCTCAAA TATGTCCACT 
TCTTCGAGTG ACTTTTCCTC CGACTGCAGC AGCTTGGTGT CACACAGATC TCGCTCACAA 
TACGATTCGA TTGATCAGCT GCGTAACTTA GATGTAGCAG CCACGTGTGG GGGCAGTATT 
GGAAACGGCG GTTATTCTTC TCGTAGCACT CAACGCCATC GCAGCTCGGT GAGTTCTTCA 
TCTTACTCAG AGAATGGACA GTACAGGCGC AGGCAGCGCC GCACGAGTTA CAACAAGCGT 
AGATCTAGAA ACCAAAAGCG GGGCAGTACT TCCTCGTCAT GCTCGGGCTC CGAAAAAAGC 
GATCGCGAAC GTAGCCGCAG CCCACACAGA AAATTGCGCC ACTCGCGGTC CCGGTCGCGC 
TCAAAATCTG ATACACGCTA CCCTAACAAC AACTCCTCCT CCAATAACAA CAATCGGCGA 
GGATTTTTTG ATCGTAACGT GTCGCAACCG GCCGTTGAAG AGCGGCGAAT CGTCTACGTC 
GGTCGCATTG AGCAAGAAAC CACCAAGGAA ATTCTGCGTC GTAAATTTCT GCCCTATGGA 
AGCATTAAAC AAATAACAAT ACACTACAAG GAAAATGGAA TGAAGTATGG GTTCGTTACG 
TACGAAAGAG CGCAGGACGC ATTCACGGCC ATTGACACGA GTCATAGAGA CTCGCAAATA 
AGCATGTATG ACATAAGTTT TGGAGGAAGA CGTGCCTTCT GTCGTTCATC TTACGCTGAC 
TTGGATAATG CTGGCATTAA TAATTACAAC TCGTATGTGT TTCCCAAGGA AGCACCAGCA 
CCGAATGTAG TCGAGGACTC TTTCGAAGCT TTGTTGCTTC AGGTAAAAGC AAAGTTAAAC 
GCGGGCAAGT CACCGGTAGG TTCATCTACG CTGGAGGCAC CATCAGCTTC CGGTACTGTA 
CTTCAGGGCC ACAGTCAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srl ( NM_141212.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649469.2 Drosophila melanogaster spargel (srl), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa03828
Clone ID Related Accession (Same CDS sequence)	NM_168996.5 , NM_141212.4
Accession Version	NM_141212.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spargel, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141212.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCAC GAATGCTAAA TGTGTTCCAA GGAGATACCT TTGAGGCAAA TTATACCAAA 
TACGAGTCTG AGCCGATATT CTGGTCATCT AATGATGAAC TGCAACTGAC AGATACACTG 
AGCGGCATCC TACCCATCGC GCCCACAGAC GAATCACTGC TCAACCATAA CCGGGAGGAA 
GATCTGCAAA ACAGAAAGAA TCTGCTGATT TACAACGACT TTCTGAAACA GGAGCTCAGC 
AACCATGATG CCAATGAGCT AAATGCCCAG GTCTATCGGT CCATCGCCGA CAGCGGGGGC 
GAGATTGCCT ATGAGCAGCA GCGCCAGATC AGCGCCATTG TGGAGTACGA CAAAGAAGAT 
ATTAAAATCG ACGGCCTTAC GGAGGAGGAG GACATACAAA GCCAGTCCAC ATTTCGCACC 
CGCACAGACA GCTCTTCGAT TGGTGACTAT GAATCGCACT CTAGCGACTA TGATGATGAA 
TTTGACATTG TCAACGAAGA AAAAAGTCCA AGGCTGCAGG TTGTCCATAA CAATATCGGC 
AACTTGCAGA GCTCCTGTGA GCGCAAGGGA CGGACGCTCA GTACTAACAC TATTATCTCC 
GAAGTAGACG CATTGGGGCT AAGTATTGAT GTTAATAACT TTGACCTAGC TGATTTTATT 
ACCAAAGACG ATTTTGCTGA GAATTTAAAC GCCTGTCGCA AAAAGGAATC TCTGCAAGCA 
CAGGCGCAAA TTAAATCTAA TACTTTAGCA GATGTTCCCA TACTTCTTAA TCCACCGGTT 
GCCACAATTG TGGCAAAACC CGGGGTCCCA TCCTCTAAAC CTGGCGATTC TGATTATGAC 
TCTGACTCGA TTATCGACGT TGAAACTGTC GATGTACTCG ATATGAATAT GGCCAATATC 
TGGTCAATAG ATAAGCCGGC AAAAATGGGC CAAACTCCCG ATGAATTACG CTATGTGGAC 
AATGTAAAGG CCGATCCCTC TTGGAGTCCG AGATCCGCAA AAAAGACCAC ACCTACTATC 
AAAGAGAATA AGCCGGAACA ACTGGGGAAC CATCAGCTCG CGGTCCCAGC ATCAAACAAA 
CCGAAGCATA AGAATATTTG CCTGGTGCCT AATAAAAAAC AGTGCGATTT TCTTAAACGT 
AAGGTTGGTA GTCCCTCCTT GTCCAAGCTA ACTAAGGCGA CAACAAAAAA AGCGACTGAG 
TCTATGAGCA ACAAGCAGCC GTCAGTAAAA AATACTCAAT GCACGGGTCT CGGATTGGGT 
GGAAAAAACC TTTCGCTTCT GAAGCAGGAG AGGGACGCCG CATTATCGGT GTGTGCTGCA 
AAACCTATAG CACCTCCAAC CCTTTCGACA ACAACCCCGA CTTTGACACC GCCAGCAAAG 
CGAAAGCTAA ATCTAGAGGA ATATAAACAG CGTCGTTGTG GCGGTGTGGG GGCTGTGTAT 
CCTAAACCAA CTCCGCCCAA GCAGGCGAAA ATAGAGGTTG CCGCCAAACG CATTGCTCCT 
ACCCCGGTTT CAAAACCAAC ACCTTCACCA AAAAAAGCGG AAATAGTCAA TATTGTAAAC 
AGCCTTAAAT GTCCTCGCAC AGAACAATCT TCTTTGCCCA TGGATCCCAT TACCATGGCC 
AAGAACAAGG TGCTGCGTAT GCTAGAGATG AAACGTGCCC AACAGCTAAA AATAATCGAT 
TCCCGCGTAT CAGCCAAGGT GCCACGCGTA ACCAAGCTGC CGCCACTTAA GGATATTGTA 
AAGGACACCT ACTGCATGGA AACTGAGGAT CCTGGCACAG AGATAGCGCC ACTCAGCAAC 
AAACTCCACC CGGACTATGA GGAGATTATA ATAGTTTCAG CTAGTTGCAA TACAGACATA 
ACAATACCTC CCAATCAATT AAGCAAGGCC TCCCCTCGCT CTCTCTTAAA GTCATCAGTT 
TTGCTGTACA ACATCTCAAA CGGCCAGGAT GCCAATAAGA ACATGAGCAA CTCCCTAATA 
GCCAGCATCC AAAGCGAGGT CGCCAGGCAA ACGAGTAACA CTACTCTCTC GACGATACAG 
TCCAACGCAA CAAAAGTTAT GTCTTCCGCT GATAAGAATT GTCAGCATGG CGAGGACATG 
GTCATCATGC ACTTACCCAA GGATCGGGTG CGAAAAACTC TTGTGAGCAT TGCAACCCAA 
ACTGATCTGC AACCCGAATT TCCCCTATTG ACGCTGCCCC CAAAGCGACA GTCTCGAGAG 
CGTACGCGTC GAAATTATCG CAGGCGACGG ACACAAGGCT CGGGCTCAAA TATGTCCACT 
TCTTCGAGTG ACTTTTCCTC CGACTGCAGC AGCTTGGTGT CACACAGATC TCGCTCACAA 
TACGATTCGA TTGATCAGCT GCGTAACTTA GATGTAGCAG CCACGTGTGG GGGCAGTATT 
GGAAACGGCG GTTATTCTTC TCGTAGCACT CAACGCCATC GCAGCTCGGT GAGTTCTTCA 
TCTTACTCAG AGAATGGACA GTACAGGCGC AGGCAGCGCC GCACGAGTTA CAACAAGCGT 
AGATCTAGAA ACCAAAAGCG GGGCAGTACT TCCTCGTCAT GCTCGGGCTC CGAAAAAAGC 
GATCGCGAAC GTAGCCGCAG CCCACACAGA AAATTGCGCC ACTCGCGGTC CCGGTCGCGC 
TCAAAATCTG ATACACGCTA CCCTAACAAC AACTCCTCCT CCAATAACAA CAATCGGCGA 
GGATTTTTTG ATCGTAACGT GTCGCAACCG GCCGTTGAAG AGCGGCGAAT CGTCTACGTC 
GGTCGCATTG AGCAAGAAAC CACCAAGGAA ATTCTGCGTC GTAAATTTCT GCCCTATGGA 
AGCATTAAAC AAATAACAAT ACACTACAAG GAAAATGGAA TGAAGTATGG GTTCGTTACG 
TACGAAAGAG CGCAGGACGC ATTCACGGCC ATTGACACGA GTCATAGAGA CTCGCAAATA 
AGCATGTATG ACATAAGTTT TGGAGGAAGA CGTGCCTTCT GTCGTTCATC TTACGCTGAC 
TTGGATAATG CTGGCATTAA TAATTACAAC TCGTATGTGT TTCCCAAGGA AGCACCAGCA 
CCGAATGTAG TCGAGGACTC TTTCGAAGCT TTGTTGCTTC AGGTAAAAGC AAAGTTAAAC 
GCGGGCAAGT CACCGGTAGG TTCATCTACG CTGGAGGCAC CATCAGCTTC CGGTACTGTA 
CTTCAGGGCC ACAGTCAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649469.2
CDS	457..3660
Translation	MDSRMLNVFQGDTFEANYTKYESEPIFWSSNDELQLTDTLSGILPIAPTDESLLNHNREEDLQNRKNLLIYNDFLKQELSNHDANELNAQVYRSIADSGGEIAYEQQRQISAIVEYDKEDIKIDGLTEEEDIQSQSTFRTRTDSSSIGDYESHSSDYDDEFDIVNEEKSPRLQVVHNNIGNLQSSCERKGRTLSTNTIISEVDALGLSIDVNNFDLADFITKDDFAENLNACRKKESLQAQAQIKSNTLADVPILLNPPVATIVAKPGVPSSKPGDSDYDSDSIIDVETVDVLDMNMANIWSIDKPAKMGQTPDELRYVDNVKADPSWSPRSAKKTTPTIKENKPEQLGNHQLAVPASNKPKHKNICLVPNKKQCDFLKRKVGSPSLSKLTKATTKKATESMSNKQPSVKNTQCTGLGLGGKNLSLLKQERDAALSVCAAKPIAPPTLSTTTPTLTPPAKRKLNLEEYKQRRCGGVGAVYPKPTPPKQAKIEVAAKRIAPTPVSKPTPSPKKAEIVNIVNSLKCPRTEQSSLPMDPITMAKNKVLRMLEMKRAQQLKIIDSRVSAKVPRVTKLPPLKDIVKDTYCMETEDPGTEIAPLSNKLHPDYEEIIIVSASCNTDITIPPNQLSKASPRSLLKSSVLLYNISNGQDANKNMSNSLIASIQSEVARQTSNTTLSTIQSNATKVMSSADKNCQHGEDMVIMHLPKDRVRKTLVSIATQTDLQPEFPLLTLPPKRQSRERTRRNYRRRRTQGSGSNMSTSSSDFSSDCSSLVSHRSRSQYDSIDQLRNLDVAATCGGSIGNGGYSSRSTQRHRSSVSSSSYSENGQYRRRQRRTSYNKRRSRNQKRGSTSSSCSGSEKSDRERSRSPHRKLRHSRSRSRSKSDTRYPNNNSSSNNNNRRGFFDRNVSQPAVEERRIVYVGRIEQETTKEILRRKFLPYGSIKQITIHYKENGMKYGFVTYERAQDAFTAIDTSHRDSQISMYDISFGGRRAFCRSSYADLDNAGINNYNSYVFPKEAPAPNVVEDSFEALLLQVKAKLNAGKSPVGSSTLEAPSASGTVLQGHSQM

Target ORF information:

RefSeq Version	NM_141212.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spargel (srl), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141212.4

ORF Insert Sequence:

ATGGATTCAC GAATGCTAAA TGTGTTCCAA GGAGATACCT TTGAGGCAAA TTATACCAAA 
TACGAGTCTG AGCCGATATT CTGGTCATCT AATGATGAAC TGCAACTGAC AGATACACTG 
AGCGGCATCC TACCCATCGC GCCCACAGAC GAATCACTGC TCAACCATAA CCGGGAGGAA 
GATCTGCAAA ACAGAAAGAA TCTGCTGATT TACAACGACT TTCTGAAACA GGAGCTCAGC 
AACCATGATG CCAATGAGCT AAATGCCCAG GTCTATCGGT CCATCGCCGA CAGCGGGGGC 
GAGATTGCCT ATGAGCAGCA GCGCCAGATC AGCGCCATTG TGGAGTACGA CAAAGAAGAT 
ATTAAAATCG ACGGCCTTAC GGAGGAGGAG GACATACAAA GCCAGTCCAC ATTTCGCACC 
CGCACAGACA GCTCTTCGAT TGGTGACTAT GAATCGCACT CTAGCGACTA TGATGATGAA 
TTTGACATTG TCAACGAAGA AAAAAGTCCA AGGCTGCAGG TTGTCCATAA CAATATCGGC 
AACTTGCAGA GCTCCTGTGA GCGCAAGGGA CGGACGCTCA GTACTAACAC TATTATCTCC 
GAAGTAGACG CATTGGGGCT AAGTATTGAT GTTAATAACT TTGACCTAGC TGATTTTATT 
ACCAAAGACG ATTTTGCTGA GAATTTAAAC GCCTGTCGCA AAAAGGAATC TCTGCAAGCA 
CAGGCGCAAA TTAAATCTAA TACTTTAGCA GATGTTCCCA TACTTCTTAA TCCACCGGTT 
GCCACAATTG TGGCAAAACC CGGGGTCCCA TCCTCTAAAC CTGGCGATTC TGATTATGAC 
TCTGACTCGA TTATCGACGT TGAAACTGTC GATGTACTCG ATATGAATAT GGCCAATATC 
TGGTCAATAG ATAAGCCGGC AAAAATGGGC CAAACTCCCG ATGAATTACG CTATGTGGAC 
AATGTAAAGG CCGATCCCTC TTGGAGTCCG AGATCCGCAA AAAAGACCAC ACCTACTATC 
AAAGAGAATA AGCCGGAACA ACTGGGGAAC CATCAGCTCG CGGTCCCAGC ATCAAACAAA 
CCGAAGCATA AGAATATTTG CCTGGTGCCT AATAAAAAAC AGTGCGATTT TCTTAAACGT 
AAGGTTGGTA GTCCCTCCTT GTCCAAGCTA ACTAAGGCGA CAACAAAAAA AGCGACTGAG 
TCTATGAGCA ACAAGCAGCC GTCAGTAAAA AATACTCAAT GCACGGGTCT CGGATTGGGT 
GGAAAAAACC TTTCGCTTCT GAAGCAGGAG AGGGACGCCG CATTATCGGT GTGTGCTGCA 
AAACCTATAG CACCTCCAAC CCTTTCGACA ACAACCCCGA CTTTGACACC GCCAGCAAAG 
CGAAAGCTAA ATCTAGAGGA ATATAAACAG CGTCGTTGTG GCGGTGTGGG GGCTGTGTAT 
CCTAAACCAA CTCCGCCCAA GCAGGCGAAA ATAGAGGTTG CCGCCAAACG CATTGCTCCT 
ACCCCGGTTT CAAAACCAAC ACCTTCACCA AAAAAAGCGG AAATAGTCAA TATTGTAAAC 
AGCCTTAAAT GTCCTCGCAC AGAACAATCT TCTTTGCCCA TGGATCCCAT TACCATGGCC 
AAGAACAAGG TGCTGCGTAT GCTAGAGATG AAACGTGCCC AACAGCTAAA AATAATCGAT 
TCCCGCGTAT CAGCCAAGGT GCCACGCGTA ACCAAGCTGC CGCCACTTAA GGATATTGTA 
AAGGACACCT ACTGCATGGA AACTGAGGAT CCTGGCACAG AGATAGCGCC ACTCAGCAAC 
AAACTCCACC CGGACTATGA GGAGATTATA ATAGTTTCAG CTAGTTGCAA TACAGACATA 
ACAATACCTC CCAATCAATT AAGCAAGGCC TCCCCTCGCT CTCTCTTAAA GTCATCAGTT 
TTGCTGTACA ACATCTCAAA CGGCCAGGAT GCCAATAAGA ACATGAGCAA CTCCCTAATA 
GCCAGCATCC AAAGCGAGGT CGCCAGGCAA ACGAGTAACA CTACTCTCTC GACGATACAG 
TCCAACGCAA CAAAAGTTAT GTCTTCCGCT GATAAGAATT GTCAGCATGG CGAGGACATG 
GTCATCATGC ACTTACCCAA GGATCGGGTG CGAAAAACTC TTGTGAGCAT TGCAACCCAA 
ACTGATCTGC AACCCGAATT TCCCCTATTG ACGCTGCCCC CAAAGCGACA GTCTCGAGAG 
CGTACGCGTC GAAATTATCG CAGGCGACGG ACACAAGGCT CGGGCTCAAA TATGTCCACT 
TCTTCGAGTG ACTTTTCCTC CGACTGCAGC AGCTTGGTGT CACACAGATC TCGCTCACAA 
TACGATTCGA TTGATCAGCT GCGTAACTTA GATGTAGCAG CCACGTGTGG GGGCAGTATT 
GGAAACGGCG GTTATTCTTC TCGTAGCACT CAACGCCATC GCAGCTCGGT GAGTTCTTCA 
TCTTACTCAG AGAATGGACA GTACAGGCGC AGGCAGCGCC GCACGAGTTA CAACAAGCGT 
AGATCTAGAA ACCAAAAGCG GGGCAGTACT TCCTCGTCAT GCTCGGGCTC CGAAAAAAGC 
GATCGCGAAC GTAGCCGCAG CCCACACAGA AAATTGCGCC ACTCGCGGTC CCGGTCGCGC 
TCAAAATCTG ATACACGCTA CCCTAACAAC AACTCCTCCT CCAATAACAA CAATCGGCGA 
GGATTTTTTG ATCGTAACGT GTCGCAACCG GCCGTTGAAG AGCGGCGAAT CGTCTACGTC 
GGTCGCATTG AGCAAGAAAC CACCAAGGAA ATTCTGCGTC GTAAATTTCT GCCCTATGGA 
AGCATTAAAC AAATAACAAT ACACTACAAG GAAAATGGAA TGAAGTATGG GTTCGTTACG 
TACGAAAGAG CGCAGGACGC ATTCACGGCC ATTGACACGA GTCATAGAGA CTCGCAAATA 
AGCATGTATG ACATAAGTTT TGGAGGAAGA CGTGCCTTCT GTCGTTCATC TTACGCTGAC 
TTGGATAATG CTGGCATTAA TAATTACAAC TCGTATGTGT TTCCCAAGGA AGCACCAGCA 
CCGAATGTAG TCGAGGACTC TTTCGAAGCT TTGTTGCTTC AGGTAAAAGC AAAGTTAAAC 
GCGGGCAAGT CACCGGTAGG TTCATCTACG CTGGAGGCAC CATCAGCTTC CGGTACTGTA 
CTTCAGGGCC ACAGTCAGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

srl ( NM_168997.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730836.3 Drosophila melanogaster spargel (srl), transcript variant D, mRNA.

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CloneID	OFa03829
Clone ID Related Accession (Same CDS sequence)	NM_168997.5
Accession Version	NM_168997.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spargel, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_168997.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCAC GAATGCTAAA TGTGTTCCAA GGAGATACCT TTGAGGCAAA TTATACCAAA 
TACGAGTCTG AGCCGATATT CTGGTCATCT AATGATGAAC TGCAACTGAC AGATACACTG 
AGCGGCATCC TACCCATCGC GCCCACAGAC GAATCACTGC TCAACCATAA CCGGGAGGAA 
GATCTGCAAA ACAGAAAGAA TCTGGAGCTC AGCAACCATG ATGCCAATGA GCTAAATGCC 
CAGGTCTATC GGTCCATCGC CGACAGCGGG GGCGAGATTG CCTATGAGCA GCAGCGCCAG 
ATCAGCGCCA TTGTGGAGTA CGACAAAGAA GATATTAAAA TCGACGGCCT TACGGAGGAG 
GAGGACATAC AAAGCCAGTC CACATTTCGC ACCCGCACAG ACAGCTCTTC GATTGGTGAC 
TATGAATCGC ACTCTAGCGA CTATGATGAT GAATTTGACA TTGTCAACGA AGAAAAAAGT 
CCAAGGCTGC AGGTTGTCCA TAACAATATC GGCAACTTGC AGAGCTCCTG TGAGCGCAAG 
GGACGGACGC TCAGTACTAA CACTATTATC TCCGAAGTAG ACGCATTGGG GCTAAGTATT 
GATGTTAATA ACTTTGACCT AGCTGATTTT ATTACCAAAG ACGATTTTGC TGAGAATTTA 
AACGCCTGTC GCAAAAAGGA ATCTCTGCAA GCACAGGCGC AAATTAAATC TAATACTTTA 
GCAGATGTTC CCATACTTCT TAATCCACCG GTTGCCACAA TTGTGGCAAA ACCCGGGGTC 
CCATCCTCTA AACCTGGCGA TTCTGATTAT GACTCTGACT CGATTATCGA CGTTGAAACT 
GTCGATGTAC TCGATATGAA TATGGCCAAT ATCTGGTCAA TAGATAAGCC GGCAAAAATG 
GGCCAAACTC CCGATGAATT ACGCTATGTG GACAATGTAA AGGCCGATCC CTCTTGGAGT 
CCGAGATCCG CAAAAAAGAC CACACCTACT ATCAAAGAGA ATAAGCCGGA ACAACTGGGG 
AACCATCAGC TCGCGGTCCC AGCATCAAAC AAACCGAAGC ATAAGAATAT TTGCCTGGTG 
CCTAATAAAA AACAGTGCGA TTTTCTTAAA CGTAAGGTTG GTAGTCCCTC CTTGTCCAAG 
CTAACTAAGG CGACAACAAA AAAAGCGACT GAGTCTATGA GCAACAAGCA GCCGTCAGTA 
AAAAATACTC AATGCACGGG TCTCGGATTG GGTGGAAAAA ACCTTTCGCT TCTGAAGCAG 
GAGAGGGACG CCGCATTATC GGTGTGTGCT GCAAAACCTA TAGCACCTCC AACCCTTTCG 
ACAACAACCC CGACTTTGAC ACCGCCAGCA AAGCGAAAGC TAAATCTAGA GGAATATAAA 
CAGCGTCGTT GTGGCGGTGT GGGGGCTGTG TATCCTAAAC CAACTCCGCC CAAGCAGGCG 
AAAATAGAGG TTGCCGCCAA ACGCATTGCT CCTACCCCGG TTTCAAAACC AACACCTTCA 
CCAAAAAAAG CGGAAATAGT CAATATTGTA AACAGCCTTA AATGTCCTCG CACAGAACAA 
TCTTCTTTGC CCATGGATCC CATTACCATG GCCAAGAACA AGGTGCTGCG TATGCTAGAG 
ATGAAACGTG CCCAACAGCT AAAAATAATC GATTCCCGCG TATCAGCCAA GGTGCCACGC 
GTAACCAAGC TGCCGCCACT TAAGGATATT GTAAAGGACA CCTACTGCAT GGAAACTGAG 
GATCCTGGCA CAGAGATAGC GCCACTCAGC AACAAACTCC ACCCGGACTA TGAGGAGATT 
ATAATAGTTT CAGCTAGTTG CAATACAGAC ATAACAATAC CTCCCAATCA ATTAAGCAAG 
GCCTCCCCTC GCTCTCTCTT AAAGTCATCA GTTTTGCTGT ACAACATCTC AAACGGCCAG 
GATGCCAATA AGAACATGAG CAACTCCCTA ATAGCCAGCA TCCAAAGCGA GGTCGCCAGG 
CAAACGAGTA ACACTACTCT CTCGACGATA CAGTCCAACG CAACAAAAGT TATGTCTTCC 
GCTGATAAGA ATTGTCAGCA TGGCGAGGAC ATGGTCATCA TGCACTTACC CAAGGATCGG 
GTGCGAAAAA CTCTTGTGAG CATTGCAACC CAAACTGATC TGCAACCCGA ATTTCCCCTA 
TTGACGCTGC CCCCAAAGCG ACAGTCTCGA GAGCGTACGC GTCGAAATTA TCGCAGGCGA 
CGGACACAAG GCTCGGGCTC AAATATGTCC ACTTCTTCGA GTGACTTTTC CTCCGACTGC 
AGCAGCTTGG TGTCACACAG ATCTCGCTCA CAATACGATT CGATTGATCA GCTGCGTAAC 
TTAGATGTAG CAGCCACGTG TGGGGGCAGT ATTGGAAACG GCGGTTATTC TTCTCGTAGC 
ACTCAACGCC ATCGCAGCTC GGTGAGTTCT TCATCTTACT CAGAGAATGG ACAGTACAGG 
CGCAGGCAGC GCCGCACGAG TTACAACAAG CGTAGATCTA GAAACCAAAA GCGGGGCAGT 
ACTTCCTCGT CATGCTCGGG CTCCGAAAAA AGCGATCGCG AACGTAGCCG CAGCCCACAC 
AGAAAATTGC GCCACTCGCG GTCCCGGTCG CGCTCAAAAT CTGATACACG CTACCCTAAC 
AACAACTCCT CCTCCAATAA CAACAATCGG CGAGGATTTT TTGATCGTAA CGTGTCGCAA 
CCGGCCGTTG AAGAGCGGCG AATCGTCTAC GTCGGTCGCA TTGAGCAAGA AACCACCAAG 
GAAATTCTGC GTCGTAAATT TCTGCCCTAT GGAAGCATTA AACAAATAAC AATACACTAC 
AAGGAAAATG GAATGAAGTA TGGGTTCGTT ACGTACGAAA GAGCGCAGGA CGCATTCACG 
GCCATTGACA CGAGTCATAG AGACTCGCAA ATAAGCATGT ATGACATAAG TTTTGGAGGA 
AGACGTGCCT TCTGTCGTTC ATCTTACGCT GACTTGGATA ATGCTGGCAT TAATAATTAC 
AACTCGTATG TGTTTCCCAA GGAAGCACCA GCACCGAATG TAGTCGAGGA CTCTTTCGAA 
GCTTTGTTGC TTCAGGTAAA AGCAAAGTTA AACGCGGGCA AGTCACCGGT AGGTTCATCT 
ACGCTGGAGG CACCATCAGC TTCCGGTACT GTACTTCAGG GCCACAGTCA GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730836.3
CDS	383..3559
Translation	MDSRMLNVFQGDTFEANYTKYESEPIFWSSNDELQLTDTLSGILPIAPTDESLLNHNREEDLQNRKNLELSNHDANELNAQVYRSIADSGGEIAYEQQRQISAIVEYDKEDIKIDGLTEEEDIQSQSTFRTRTDSSSIGDYESHSSDYDDEFDIVNEEKSPRLQVVHNNIGNLQSSCERKGRTLSTNTIISEVDALGLSIDVNNFDLADFITKDDFAENLNACRKKESLQAQAQIKSNTLADVPILLNPPVATIVAKPGVPSSKPGDSDYDSDSIIDVETVDVLDMNMANIWSIDKPAKMGQTPDELRYVDNVKADPSWSPRSAKKTTPTIKENKPEQLGNHQLAVPASNKPKHKNICLVPNKKQCDFLKRKVGSPSLSKLTKATTKKATESMSNKQPSVKNTQCTGLGLGGKNLSLLKQERDAALSVCAAKPIAPPTLSTTTPTLTPPAKRKLNLEEYKQRRCGGVGAVYPKPTPPKQAKIEVAAKRIAPTPVSKPTPSPKKAEIVNIVNSLKCPRTEQSSLPMDPITMAKNKVLRMLEMKRAQQLKIIDSRVSAKVPRVTKLPPLKDIVKDTYCMETEDPGTEIAPLSNKLHPDYEEIIIVSASCNTDITIPPNQLSKASPRSLLKSSVLLYNISNGQDANKNMSNSLIASIQSEVARQTSNTTLSTIQSNATKVMSSADKNCQHGEDMVIMHLPKDRVRKTLVSIATQTDLQPEFPLLTLPPKRQSRERTRRNYRRRRTQGSGSNMSTSSSDFSSDCSSLVSHRSRSQYDSIDQLRNLDVAATCGGSIGNGGYSSRSTQRHRSSVSSSSYSENGQYRRRQRRTSYNKRRSRNQKRGSTSSSCSGSEKSDRERSRSPHRKLRHSRSRSRSKSDTRYPNNNSSSNNNNRRGFFDRNVSQPAVEERRIVYVGRIEQETTKEILRRKFLPYGSIKQITIHYKENGMKYGFVTYERAQDAFTAIDTSHRDSQISMYDISFGGRRAFCRSSYADLDNAGINNYNSYVFPKEAPAPNVVEDSFEALLLQVKAKLNAGKSPVGSSTLEAPSASGTVLQGHSQM

Target ORF information:

RefSeq Version	NM_168997.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spargel (srl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168997.5

ORF Insert Sequence:

ATGGATTCAC GAATGCTAAA TGTGTTCCAA GGAGATACCT TTGAGGCAAA TTATACCAAA 
TACGAGTCTG AGCCGATATT CTGGTCATCT AATGATGAAC TGCAACTGAC AGATACACTG 
AGCGGCATCC TACCCATCGC GCCCACAGAC GAATCACTGC TCAACCATAA CCGGGAGGAA 
GATCTGCAAA ACAGAAAGAA TCTGGAGCTC AGCAACCATG ATGCCAATGA GCTAAATGCC 
CAGGTCTATC GGTCCATCGC CGACAGCGGG GGCGAGATTG CCTATGAGCA GCAGCGCCAG 
ATCAGCGCCA TTGTGGAGTA CGACAAAGAA GATATTAAAA TCGACGGCCT TACGGAGGAG 
GAGGACATAC AAAGCCAGTC CACATTTCGC ACCCGCACAG ACAGCTCTTC GATTGGTGAC 
TATGAATCGC ACTCTAGCGA CTATGATGAT GAATTTGACA TTGTCAACGA AGAAAAAAGT 
CCAAGGCTGC AGGTTGTCCA TAACAATATC GGCAACTTGC AGAGCTCCTG TGAGCGCAAG 
GGACGGACGC TCAGTACTAA CACTATTATC TCCGAAGTAG ACGCATTGGG GCTAAGTATT 
GATGTTAATA ACTTTGACCT AGCTGATTTT ATTACCAAAG ACGATTTTGC TGAGAATTTA 
AACGCCTGTC GCAAAAAGGA ATCTCTGCAA GCACAGGCGC AAATTAAATC TAATACTTTA 
GCAGATGTTC CCATACTTCT TAATCCACCG GTTGCCACAA TTGTGGCAAA ACCCGGGGTC 
CCATCCTCTA AACCTGGCGA TTCTGATTAT GACTCTGACT CGATTATCGA CGTTGAAACT 
GTCGATGTAC TCGATATGAA TATGGCCAAT ATCTGGTCAA TAGATAAGCC GGCAAAAATG 
GGCCAAACTC CCGATGAATT ACGCTATGTG GACAATGTAA AGGCCGATCC CTCTTGGAGT 
CCGAGATCCG CAAAAAAGAC CACACCTACT ATCAAAGAGA ATAAGCCGGA ACAACTGGGG 
AACCATCAGC TCGCGGTCCC AGCATCAAAC AAACCGAAGC ATAAGAATAT TTGCCTGGTG 
CCTAATAAAA AACAGTGCGA TTTTCTTAAA CGTAAGGTTG GTAGTCCCTC CTTGTCCAAG 
CTAACTAAGG CGACAACAAA AAAAGCGACT GAGTCTATGA GCAACAAGCA GCCGTCAGTA 
AAAAATACTC AATGCACGGG TCTCGGATTG GGTGGAAAAA ACCTTTCGCT TCTGAAGCAG 
GAGAGGGACG CCGCATTATC GGTGTGTGCT GCAAAACCTA TAGCACCTCC AACCCTTTCG 
ACAACAACCC CGACTTTGAC ACCGCCAGCA AAGCGAAAGC TAAATCTAGA GGAATATAAA 
CAGCGTCGTT GTGGCGGTGT GGGGGCTGTG TATCCTAAAC CAACTCCGCC CAAGCAGGCG 
AAAATAGAGG TTGCCGCCAA ACGCATTGCT CCTACCCCGG TTTCAAAACC AACACCTTCA 
CCAAAAAAAG CGGAAATAGT CAATATTGTA AACAGCCTTA AATGTCCTCG CACAGAACAA 
TCTTCTTTGC CCATGGATCC CATTACCATG GCCAAGAACA AGGTGCTGCG TATGCTAGAG 
ATGAAACGTG CCCAACAGCT AAAAATAATC GATTCCCGCG TATCAGCCAA GGTGCCACGC 
GTAACCAAGC TGCCGCCACT TAAGGATATT GTAAAGGACA CCTACTGCAT GGAAACTGAG 
GATCCTGGCA CAGAGATAGC GCCACTCAGC AACAAACTCC ACCCGGACTA TGAGGAGATT 
ATAATAGTTT CAGCTAGTTG CAATACAGAC ATAACAATAC CTCCCAATCA ATTAAGCAAG 
GCCTCCCCTC GCTCTCTCTT AAAGTCATCA GTTTTGCTGT ACAACATCTC AAACGGCCAG 
GATGCCAATA AGAACATGAG CAACTCCCTA ATAGCCAGCA TCCAAAGCGA GGTCGCCAGG 
CAAACGAGTA ACACTACTCT CTCGACGATA CAGTCCAACG CAACAAAAGT TATGTCTTCC 
GCTGATAAGA ATTGTCAGCA TGGCGAGGAC ATGGTCATCA TGCACTTACC CAAGGATCGG 
GTGCGAAAAA CTCTTGTGAG CATTGCAACC CAAACTGATC TGCAACCCGA ATTTCCCCTA 
TTGACGCTGC CCCCAAAGCG ACAGTCTCGA GAGCGTACGC GTCGAAATTA TCGCAGGCGA 
CGGACACAAG GCTCGGGCTC AAATATGTCC ACTTCTTCGA GTGACTTTTC CTCCGACTGC 
AGCAGCTTGG TGTCACACAG ATCTCGCTCA CAATACGATT CGATTGATCA GCTGCGTAAC 
TTAGATGTAG CAGCCACGTG TGGGGGCAGT ATTGGAAACG GCGGTTATTC TTCTCGTAGC 
ACTCAACGCC ATCGCAGCTC GGTGAGTTCT TCATCTTACT CAGAGAATGG ACAGTACAGG 
CGCAGGCAGC GCCGCACGAG TTACAACAAG CGTAGATCTA GAAACCAAAA GCGGGGCAGT 
ACTTCCTCGT CATGCTCGGG CTCCGAAAAA AGCGATCGCG AACGTAGCCG CAGCCCACAC 
AGAAAATTGC GCCACTCGCG GTCCCGGTCG CGCTCAAAAT CTGATACACG CTACCCTAAC 
AACAACTCCT CCTCCAATAA CAACAATCGG CGAGGATTTT TTGATCGTAA CGTGTCGCAA 
CCGGCCGTTG AAGAGCGGCG AATCGTCTAC GTCGGTCGCA TTGAGCAAGA AACCACCAAG 
GAAATTCTGC GTCGTAAATT TCTGCCCTAT GGAAGCATTA AACAAATAAC AATACACTAC 
AAGGAAAATG GAATGAAGTA TGGGTTCGTT ACGTACGAAA GAGCGCAGGA CGCATTCACG 
GCCATTGACA CGAGTCATAG AGACTCGCAA ATAAGCATGT ATGACATAAG TTTTGGAGGA 
AGACGTGCCT TCTGTCGTTC ATCTTACGCT GACTTGGATA ATGCTGGCAT TAATAATTAC 
AACTCGTATG TGTTTCCCAA GGAAGCACCA GCACCGAATG TAGTCGAGGA CTCTTTCGAA 
GCTTTGTTGC TTCAGGTAAA AGCAAAGTTA AACGCGGGCA AGTCACCGGT AGGTTCATCT 
ACGCTGGAGG CACCATCAGC TTCCGGTACT GTACTTCAGG GCCACAGTCA GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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srl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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