eRF1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eRF1
Entrez Gene ID	40240
Full Name	eukaryotic release factor 1
Synonyms	CG5605,Dm0184,Dmel\CG5605,Group J,group J,l(3)00103,l(3)05637,l(3)77ABb,l(3)neo28
General protein information	Preferred Names eukaryotic release factor 1 Names CG5605 gene product from transcript CG5605-RI CG5605-PA CG5605-PB CG5605-PC CG5605-PE CG5605-PF CG5605-PG CG5605-PH CG5605-PI eRF1-PA eRF1-PB eRF1-PC eRF1-PE eRF1-PF eRF1-PG eRF1-PH eRF1-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77B4-77B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275175.1	NP_001262104.1	eukaryotic translation release factor 1, isoform H
NM_168846.2	NP_730518.1	eukaryotic translation release factor 1, isoform C
NM_168847.2	NP_730519.1	eukaryotic translation release factor 1, isoform E
NM_176369.3	NP_788547.1	eukaryotic translation release factor 1, isoform G
NM_001275176.1	NP_001262105.1	eukaryotic translation release factor 1, isoform I
NM_140953.3	NP_649210.1	eukaryotic translation release factor 1, isoform A
NM_168845.2	NP_730517.1	eukaryotic translation release factor 1, isoform B
NM_168848.2	NP_730520.1	eukaryotic translation release factor 1, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ETF1	XP_001171542.1
Canis lupus familiaris (dog)	ETF1	NP_001239075.1
Bos taurus (cattle)	ETF1	NP_001069722.1
Mus musculus (house mouse)	Etf1	NP_659115.3
Caenorhabditis elegans (roundworm)	etf-1	NP_001024107.2
Schizosaccharomyces pombe (fission yeast)	sup45	NP_594680.1
Homo sapiens (human)	ETF1	NP_004721.1
	ETF1	XP_001112899.2
Arabidopsis thaliana (thale cress)	ERF1-1	NP_199599.1
Xenopus tropicalis (tropical clawed frog)	etf1	NP_989035.1
Gallus gallus (chicken)	ETF1	XP_414511.2
Danio rerio (zebrafish)	etf1	NP_958868.1
Drosophila melanogaster (fruit fly)	eRF1	NP_649210.1
Magnaporthe oryzae (rice blast fungus)	MGG_06266	XP_003717326.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010310	XP_319502.1
Saccharomyces cerevisiae (baker's yeast)	SUP45	NP_009701.3
Eremothecium gossypii	AGOS_AER028C	NP_984888.1
Rattus norvegicus (Norway rat)	Etf1	NP_001008345.1
Kluyveromyces lactis	KLLA0C11231g	XP_452701.1
Neurospora crassa	NCU00410	XP_957296.1
Oryza sativa (rice)	Os01g0939500	NP_001045349.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19096	NM_140953.3 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19096	NM_168845.2 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19096	NM_168846.2 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19096	NM_168847.2 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19096	NM_168848.2 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa19096	NM_176369.3 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21126	NM_001275175.1 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21132	NM_001275176.1 Latest version!	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eRF1 ( NM_168846.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730518.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19096
Clone ID Related Accession (Same CDS sequence)	NM_168846.2 , NM_168847.2 , NM_176369.3 , NM_140953.3 , NM_168845.2 , NM_168848.2
Accession Version	NM_168846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168846.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730518.1
CDS	211..1527
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDGFDLDDY

Target ORF information:

RefSeq Version	NM_168846.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168846.2

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_168847.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730519.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa19096
Clone ID Related Accession (Same CDS sequence)	NM_168846.2 , NM_168847.2 , NM_176369.3 , NM_140953.3 , NM_168845.2 , NM_168848.2
Accession Version	NM_168847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168847.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730519.1
CDS	476..1792
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDGFDLDDY

Target ORF information:

RefSeq Version	NM_168847.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168847.2

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_176369.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788547.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa19096
Clone ID Related Accession (Same CDS sequence)	NM_168846.2 , NM_168847.2 , NM_176369.3 , NM_140953.3 , NM_168845.2 , NM_168848.2
Accession Version	NM_176369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_176369.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788547.1
CDS	185..1501
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDGFDLDDY

Target ORF information:

RefSeq Version	NM_176369.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176369.3

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_140953.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649210.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant A, mRNA.

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CloneID	OFa19096
Clone ID Related Accession (Same CDS sequence)	NM_168846.2 , NM_168847.2 , NM_176369.3 , NM_140953.3 , NM_168845.2 , NM_168848.2
Accession Version	NM_140953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140953.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649210.1
CDS	767..2083
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDGFDLDDY

Target ORF information:

RefSeq Version	NM_140953.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140953.3

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_168845.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730517.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant B, mRNA.

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CloneID	OFa19096
Clone ID Related Accession (Same CDS sequence)	NM_168846.2 , NM_168847.2 , NM_176369.3 , NM_140953.3 , NM_168845.2 , NM_168848.2
Accession Version	NM_168845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168845.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730517.1
CDS	247..1563
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDGFDLDDY

Target ORF information:

RefSeq Version	NM_168845.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168845.2

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_168848.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730520.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa19096
Clone ID Related Accession (Same CDS sequence)	NM_168846.2 , NM_168847.2 , NM_176369.3 , NM_140953.3 , NM_168845.2 , NM_168848.2
Accession Version	NM_168848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168848.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730520.1
CDS	234..1550
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDGFDLDDY

Target ORF information:

RefSeq Version	NM_168848.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168848.2

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGTATCT TACGCTACAA GGTGGATTTC 
CAGAGTATGC AGCTCGATGA ATTGGACAAT GATGGCTTCG ATCTAGATGA TTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_001275175.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262104.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa21126
Clone ID Related Accession (Same CDS sequence)	NM_001275175.1
Accession Version	NM_001275175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGCATTT TGCGCTATAA AGTAGATTTC 
CAATCCCTAC AGGCCGATGA GCCGCTGGAG GATGTCGATC TCGATGACTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262104.1
CDS	211..1524
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSLQADEPLEDVDLDDY

Target ORF information:

RefSeq Version	NM_001275175.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275175.1

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGCATTT TGCGCTATAA AGTAGATTTC 
CAATCCCTAC AGGCCGATGA GCCGCTGGAG GATGTCGATC TCGATGACTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eRF1 ( NM_001275176.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262105.1 Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant I, mRNA.

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CloneID	OFa21132
Clone ID Related Accession (Same CDS sequence)	NM_001275176.1
Accession Version	NM_001275176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation release factor 1, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGCACAC TGGAAACAGT TTACTGCGAG 
ATTCCCACTT CATTAGTTGG CAATGCACGT GGACGACAGC AAAAAGCACT CGTAGTACTT 
GTTTCGCCCT TGATTTGCAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262105.1
CDS	211..1554
Translation	MSGEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGTLETVYCEIPTSLVGNARGRQQKALVVLVSPLICI

Target ORF information:

RefSeq Version	NM_001275176.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation release factor 1 (eRF1), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275176.1

ORF Insert Sequence:

ATGTCTGGCG AGGAAACGTC TGCCGATCGC AATGTCGAGA TCTGGAAAAT CAAGAAGCTC 
ATCAAGAGCC TGGAAATGGC CCGCGGCAAT GGAACCAGCA TGATTTCTTT GATTATTCCG 
CCAAAGGATC AAATCTCGCG CGTCAGCAAG ATGTTGGCCG ATGAGTTTGG AACGGCGTCG 
AACATCAAGT CGCGTGTAAA TCGGTTGTCC GTCCTCGGTG CCATTACGTC GGTACAGCAC 
AGACTCAAAT TATACACCAA AGTGCCTCCC AACGGTTTGG TCATCTACTG CGGCACAATA 
GTCACAGAGG AGGGCAAGGA GAAGAAGGTG AACATAGACT TTGAGCCATT CAAGCCCATA 
AACACGTCGC TCTACCTCTG CGACAACAAG TTCCACACGG AGGCCCTCAC TGCCCTGCTC 
GCCGACGACA ACAAATTTGG ATTCATCGTG ATGGATGGTA ACGGAGCGCT ATTCGGTACC 
CTTCAGGGCA ACACGCGCGA GGTGCTCCAC AAGTTCACCG TCGATCTGCC GAAGAAGCAC 
GGTCGTGGTG GTCAGTCCGC CCTTCGTTTC GCCCGTCTGC GTATGGAGAA GCGCCACAAC 
TACGTGCGGA AGGTCGCCGA GGTGGCCACC CAGCTCTTCA TCACGAACGA CAAGCCCAAC 
ATTGCCGGAC TCATCCTGGC TGGTAGTGCG GATTTCAAGA CTGAGCTTAG TCAGTCTGAT 
ATGTTCGATC CTCGTTTGCA ATCAAAAGTC ATCAAGCTGG TGGACGTGTC GTATGGTGGG 
GAAAACGGTT TTAACCAGGC CATTGAACTG GCGGCCGAAT CATTGCAGAA CGTTAAATTC 
ATACAGGAGA AGAAACTCAT TGGTCGCTAC TTTGATGAAA TTTCTCAGGA TACTGGCAAA 
TACTGTTTTG GAGTGGAGGA CACTTTGCGG GCACTGGAAC TTGGCTCTGT AGAGACTCTC 
ATTTGTTGGG AGAACCTGGA TATTCAACGT TATGTTCTCA AGAATCATGC CAACTCGACG 
TCAACGACAG TATTACATTT GACGCCCGAG CAGGAAAAGG ACAAGTCGCA CTTCACTGAC 
AAGGAGAGCG GGGTAGAAAT GGAGCTGATT GAGTCTCAGC CGCTGCTGGA ATGGCTGGCA 
AACAACTACA AAATGTTCGG CGCCACACTG GAGATTATCA CGGATAAGTC CCAGGAAGGA 
AGTCAGTTCG TGCGAGGTTT CGGTGGAATC GGCGGCACAC TGGAAACAGT TTACTGCGAG 
ATTCCCACTT CATTAGTTGG CAATGCACGT GGACGACAGC AAAAAGCACT CGTAGTACTT 
GTTTCGCCCT TGATTTGCAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eRF1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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