wnd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnd
Entrez Gene ID	40143
Full Name	wallenda
Synonyms	CG8789,DLK,DLK/ZPK,Dmel\CG8789,MAPKKK,Wnd,Wnd/DLK
General protein information	Preferred Names wallenda Names CG8789 gene product from transcript CG8789-RC CG8789-PA CG8789-PB CG8789-PC CG8789-PD dual leucine zipper-bearing kinase wnd-PA wnd-PB wnd-PC wnd-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76B9-76B9

mRNA and Protein(s)

mRNA	Protein	Name
NM_176362.2	NP_788540.1	wallenda, isoform B
NM_176363.3	NP_788541.1	wallenda, isoform C
NM_140880.3	NP_649137.3	wallenda, isoform A
NM_001202203.1	NP_001189132.1	wallenda, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	wnd	NP_649137.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006461	XP_316502.4
Caenorhabditis elegans (roundworm)	dlk-1	NP_001021445.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons
Title: Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons.

Here the authors identify the Dual Leucine-zipper Kinase (DLK, Wnd in Drosophila) as a critical target and effector of cAMP in injured axons.
Title: An evolutionarily conserved mechanism for cAMP elicited axonal regeneration involves direct activation of the dual leucine zipper kinase DLK.

our study in Drosophila has uncovered a novel physiological function for Wnd in modulating cell invasion
Title: Rho1-Wnd signaling regulates loss-of-cell polarity-induced cell invasion in Drosophila.

DLK is not essential for dendrite regeneration.
Title: Dendrite injury triggers DLK-independent regeneration.

The results of this study indicated that Wnd regulates multiple independent pathways in Drosophila motoneurons and that JIP1 scaffolds a specific downstream cascade required for the organization of presynaptic microtubules during synaptic development.
Title: Independent pathways downstream of the Wnd/DLK MAPKKK regulate synaptic structure, axonal transport, and injury signaling.

The following wnd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18994	NM_176363.3 Latest version!	Drosophila melanogaster wallenda (wnd), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18994	NM_140880.3 Latest version!	Drosophila melanogaster wallenda (wnd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18994	NM_176362.2 Latest version!	Drosophila melanogaster wallenda (wnd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21089	NM_001202203.1 Latest version!	Drosophila melanogaster wallenda (wnd), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnd ( NM_176362.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788540.1 Drosophila melanogaster wallenda (wnd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18994
Clone ID Related Accession (Same CDS sequence)	NM_176362.2 , NM_176363.3 , NM_140880.3
Accession Version	NM_176362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wallenda, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_176362.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCGT TCAGCGACAG TCTGAGCAAA AGTCGCGATG ATCTGGTCAC CGCCGCCTCC 
CGGCAACAAC AACAGCTACA CGGACGGAGG CGACACCACG GCAGCAGCCC CAACTTGTCG 
CTCGATCAGA CCGACAATCT GCGAAGGAGC ATGGCTTGCC TGCAGGATGA GTTCGGTCAC 
CTAGGCATCG CCGCTACAGA CCTGCCGTTC AAATCCTCCG ACCTGGACTC TCCGCCCCGC 
CTGCAGCATC ACAACAACTA TGCGGAGATC ACGGACAGCA GTGCGGAAAA CACGTGCCAA 
CAGCGCTGGC CACCTCATGT GGGAGGAGCA GGGGCGGCCT TCGGGCATCC GGACAAGCCC 
ATTGGCTGGA TGTACGGACT GCTCGGATGC ATGAAGCCCG TGCTTTCGTT CATCGGGAAG 
ACGGGGGTGA TCGAGGTGAA GAGTCAGCGG AGCGAGGACT GGCAGATTCC ATTTGAGTCC 
ATCACCGAGT TGGAATGGCT GGGAAGCGGA GCACAAGGAG CCGTCTTCAG TGGCAGGCTA 
AAGAACGAGA CTGTGGCGGT CAAGAAGGTG AAGGAGCTGA AGGAAACGGA CATCAAGCAT 
CTCAGGAAGC TCGATCATGA GAACATTATT AAGTTCAAAG GGGTTTGCAC GCAGTCACCG 
GTGTTCTGCA TCATCATGGA GTTCTGTCCG TATGGCCCAC TGCAGAATAT CCTGAAGGAG 
GAGCAAGTCA TGTTACCGTC CCGCTTGGTC TCTTGGTCCA AGCAAATTGC CCTGGGCATG 
CAGTATCTGC ATTCCCACAA GATCATACAC CGAGATCTAA AGAGTCCAAA CATACTGATT 
AGTACAAATG AGGTGGTGAA GATCAGCGAC TTTGGCACCA GTCGCGAGTG GAACGAGATC 
AGCACCAAGA TGAGCTTTGC TGGTACCGTA GCCTGGATGG CTCCGGAAGT GATCAGGAAT 
GAGCCGTGCT CCGAGAAGGT GGACATTTGG TCGTACGGTG TGGTGCTATG GGAAATGCTC 
ACCTGCGAGA TACCTTACAA GGATGTGGAC TCATCAGCCA TAATCTGGGG TGTGGGCAAC 
AACTCTCTCA AGCTGCTGGT CCCGAGCACC TGTCCGGAGG GATTTAAGTT GCTGGTTAAG 
CTCTGTTGGA AAAGTAAGCC CCGAAATCGG CCATCGTTCC GGCAGATACT CTCGCACTTG 
GATATCGCCG GACCGGAACT GCTGAGAAAA ACCGAGAAGC AATATTTTGA GACGCAAAAG 
TCGTGGAAGG AGGAGGTGAG ATCTCACTTG AAGGAGATTA CCCAAAATGG CACCAACATA 
CACAAGTACG AGCAAGATCT GATTAAGCGG CGGACGGCAG AGTGGCGACA TGCCCAGGAT 
ATCCGAATGG TGTACGAGGA TAAGTTGCAA AAGACCAACC AGCTTTTCTT CGAACTCAGC 
GAGTGCATGT CCCAGTTGCA GGAAAAGGAG AAGGAAATCG CAGAACGAGA ACGGAAACTA 
CCAGGAAGTG GCTACAAACC CAATCGACGT TTTGGCAACA CGATCCGAAA AATGCAACAC 
TATCGCCGAC GCCTGAACCC TGCTCCTGCC GCCATTCAGC AACAATCAAC AACGCCGGAT 
CCAGAGACTA CGCCGGAGTC CCCTGTGAAG TGTATGCTAT ATGCCCAGCT TGATAGCAAT 
TGCCAACCCA AATCGTATCT GGCCAATATC ATCCCGAGTA GCGGCTTAGG CGCACCCATG 
CCCAATAAAA ACAAGAAGGT CTTCCGGCAC CGACGCAACG CATCTGGTTC CTTCGGTGCA 
CCACCCAAGT ACAGTCCGAC GAGGGATCGT CGTTACCAGA GCGAACCGGA GAACCGAAAG 
GTGCAGCTGG TGGAGAGACA AACCCAAACA GATGCCATGG ACGTGAGTGA AACGGATATT 
AGTCCTAGCG CGGAGGCTCC TCGATCTCAG CCCATCGACG TGCCAGTTCC GAATCATCGC 
CAACTGCCGC TGCAACTGCA GAGAGTACAG AAAATTGCCC AAGCTCAGGC GAGGGCTCGA 
AGTGGAAGCA CTTCATCTGC AGCTGGCGCC GTGAATCCCG CTTGTCCGTC GAATGGCAAC 
TCTCTGAGCA CAAGCGAGCT GACCTACCAA GATGCCTGCT CTAGTCCCGA TCAGCTCATC 
GACGATGTAA TGAACAGCAA CGAACGTTTG GACATGACTG AATGCTGCAG TGACAACGAA 
AACCTGGAAC GTCTAGGCCG CAAAGTCATC GAATTCATCA ACGAAAACCG CCTGTCCATT 
CAGTCAAACA CGAACTCCGT GAGCAATGCC GATAATGGGA ATGGAGGAGC CTCTCCTCTT 
GAATTGCGTG AGAGTGGTAA CAGTCCGTGT TTAAGTCGAT GCAGCAGCAC ACATAGCAAA 
CGGCGGAAGC ATCCACTGGG GGACAATCCA AACGGCAGTT CGATAGGCAA TGCCAACGGG 
GAGTCGCATG AGCAGGACAG CTGGTCGGAC GAGGAGGGCG AGACCACGGA CTACAAGTAC 
GCTCTGCGGA GAAGAAGCAT TGGCCGCCAG CCCATAGCTC GTGGCATGCG ACCCCGTCGC 
AGCTACAAGG CTCCACTCTC CCAGAAGATT GCCATCCACA AGCGCAATGT GGTAATCGTA 
TCAGACGAGG AGGAAAACAC CTCCGAGTAC AGTCACTCAC CCTCCAGCCA GCACTCGACA 
CTAGAGAGCA ATACCGACAT TGCGGACATG AAGAAAACTC AGGCCACATC CACCTCAAGC 
AACAGCTATA GCGAGCCCGA AGACGATTCG AGTGATTCCA GCGATGAGGA GCAGGGAAAC 
CGGCCGACGG AAGCCAAGGC CGAGGGTCCA GCTTTGCCTA TGGGCGCCGT ACGCAGCAGC 
GATATTATAT CCATACCTAC TTTTGAGGCT GATGGCGCCG TCAATATGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788540.1
CDS	232..3165
Translation	MQPFSDSLSKSRDDLVTAASRQQQQLHGRRRHHGSSPNLSLDQTDNLRRSMACLQDEFGHLGIAATDLPFKSSDLDSPPRLQHHNNYAEITDSSAENTCQQRWPPHVGGAGAAFGHPDKPIGWMYGLLGCMKPVLSFIGKTGVIEVKSQRSEDWQIPFESITELEWLGSGAQGAVFSGRLKNETVAVKKVKELKETDIKHLRKLDHENIIKFKGVCTQSPVFCIIMEFCPYGPLQNILKEEQVMLPSRLVSWSKQIALGMQYLHSHKIIHRDLKSPNILISTNEVVKISDFGTSREWNEISTKMSFAGTVAWMAPEVIRNEPCSEKVDIWSYGVVLWEMLTCEIPYKDVDSSAIIWGVGNNSLKLLVPSTCPEGFKLLVKLCWKSKPRNRPSFRQILSHLDIAGPELLRKTEKQYFETQKSWKEEVRSHLKEITQNGTNIHKYEQDLIKRRTAEWRHAQDIRMVYEDKLQKTNQLFFELSECMSQLQEKEKEIAERERKLPGSGYKPNRRFGNTIRKMQHYRRRLNPAPAAIQQQSTTPDPETTPESPVKCMLYAQLDSNCQPKSYLANIIPSSGLGAPMPNKNKKVFRHRRNASGSFGAPPKYSPTRDRRYQSEPENRKVQLVERQTQTDAMDVSETDISPSAEAPRSQPIDVPVPNHRQLPLQLQRVQKIAQAQARARSGSTSSAAGAVNPACPSNGNSLSTSELTYQDACSSPDQLIDDVMNSNERLDMTECCSDNENLERLGRKVIEFINENRLSIQSNTNSVSNADNGNGGASPLELRESGNSPCLSRCSSTHSKRRKHPLGDNPNGSSIGNANGESHEQDSWSDEEGETTDYKYALRRRSIGRQPIARGMRPRRSYKAPLSQKIAIHKRNVVIVSDEEENTSEYSHSPSSQHSTLESNTDIADMKKTQATSTSSNSYSEPEDDSSDSSDEEQGNRPTEAKAEGPALPMGAVRSSDIISIPTFEADGAVNMV

Target ORF information:

RefSeq Version	NM_176362.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wallenda (wnd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176362.2

ORF Insert Sequence:

ATGCAACCGT TCAGCGACAG TCTGAGCAAA AGTCGCGATG ATCTGGTCAC CGCCGCCTCC 
CGGCAACAAC AACAGCTACA CGGACGGAGG CGACACCACG GCAGCAGCCC CAACTTGTCG 
CTCGATCAGA CCGACAATCT GCGAAGGAGC ATGGCTTGCC TGCAGGATGA GTTCGGTCAC 
CTAGGCATCG CCGCTACAGA CCTGCCGTTC AAATCCTCCG ACCTGGACTC TCCGCCCCGC 
CTGCAGCATC ACAACAACTA TGCGGAGATC ACGGACAGCA GTGCGGAAAA CACGTGCCAA 
CAGCGCTGGC CACCTCATGT GGGAGGAGCA GGGGCGGCCT TCGGGCATCC GGACAAGCCC 
ATTGGCTGGA TGTACGGACT GCTCGGATGC ATGAAGCCCG TGCTTTCGTT CATCGGGAAG 
ACGGGGGTGA TCGAGGTGAA GAGTCAGCGG AGCGAGGACT GGCAGATTCC ATTTGAGTCC 
ATCACCGAGT TGGAATGGCT GGGAAGCGGA GCACAAGGAG CCGTCTTCAG TGGCAGGCTA 
AAGAACGAGA CTGTGGCGGT CAAGAAGGTG AAGGAGCTGA AGGAAACGGA CATCAAGCAT 
CTCAGGAAGC TCGATCATGA GAACATTATT AAGTTCAAAG GGGTTTGCAC GCAGTCACCG 
GTGTTCTGCA TCATCATGGA GTTCTGTCCG TATGGCCCAC TGCAGAATAT CCTGAAGGAG 
GAGCAAGTCA TGTTACCGTC CCGCTTGGTC TCTTGGTCCA AGCAAATTGC CCTGGGCATG 
CAGTATCTGC ATTCCCACAA GATCATACAC CGAGATCTAA AGAGTCCAAA CATACTGATT 
AGTACAAATG AGGTGGTGAA GATCAGCGAC TTTGGCACCA GTCGCGAGTG GAACGAGATC 
AGCACCAAGA TGAGCTTTGC TGGTACCGTA GCCTGGATGG CTCCGGAAGT GATCAGGAAT 
GAGCCGTGCT CCGAGAAGGT GGACATTTGG TCGTACGGTG TGGTGCTATG GGAAATGCTC 
ACCTGCGAGA TACCTTACAA GGATGTGGAC TCATCAGCCA TAATCTGGGG TGTGGGCAAC 
AACTCTCTCA AGCTGCTGGT CCCGAGCACC TGTCCGGAGG GATTTAAGTT GCTGGTTAAG 
CTCTGTTGGA AAAGTAAGCC CCGAAATCGG CCATCGTTCC GGCAGATACT CTCGCACTTG 
GATATCGCCG GACCGGAACT GCTGAGAAAA ACCGAGAAGC AATATTTTGA GACGCAAAAG 
TCGTGGAAGG AGGAGGTGAG ATCTCACTTG AAGGAGATTA CCCAAAATGG CACCAACATA 
CACAAGTACG AGCAAGATCT GATTAAGCGG CGGACGGCAG AGTGGCGACA TGCCCAGGAT 
ATCCGAATGG TGTACGAGGA TAAGTTGCAA AAGACCAACC AGCTTTTCTT CGAACTCAGC 
GAGTGCATGT CCCAGTTGCA GGAAAAGGAG AAGGAAATCG CAGAACGAGA ACGGAAACTA 
CCAGGAAGTG GCTACAAACC CAATCGACGT TTTGGCAACA CGATCCGAAA AATGCAACAC 
TATCGCCGAC GCCTGAACCC TGCTCCTGCC GCCATTCAGC AACAATCAAC AACGCCGGAT 
CCAGAGACTA CGCCGGAGTC CCCTGTGAAG TGTATGCTAT ATGCCCAGCT TGATAGCAAT 
TGCCAACCCA AATCGTATCT GGCCAATATC ATCCCGAGTA GCGGCTTAGG CGCACCCATG 
CCCAATAAAA ACAAGAAGGT CTTCCGGCAC CGACGCAACG CATCTGGTTC CTTCGGTGCA 
CCACCCAAGT ACAGTCCGAC GAGGGATCGT CGTTACCAGA GCGAACCGGA GAACCGAAAG 
GTGCAGCTGG TGGAGAGACA AACCCAAACA GATGCCATGG ACGTGAGTGA AACGGATATT 
AGTCCTAGCG CGGAGGCTCC TCGATCTCAG CCCATCGACG TGCCAGTTCC GAATCATCGC 
CAACTGCCGC TGCAACTGCA GAGAGTACAG AAAATTGCCC AAGCTCAGGC GAGGGCTCGA 
AGTGGAAGCA CTTCATCTGC AGCTGGCGCC GTGAATCCCG CTTGTCCGTC GAATGGCAAC 
TCTCTGAGCA CAAGCGAGCT GACCTACCAA GATGCCTGCT CTAGTCCCGA TCAGCTCATC 
GACGATGTAA TGAACAGCAA CGAACGTTTG GACATGACTG AATGCTGCAG TGACAACGAA 
AACCTGGAAC GTCTAGGCCG CAAAGTCATC GAATTCATCA ACGAAAACCG CCTGTCCATT 
CAGTCAAACA CGAACTCCGT GAGCAATGCC GATAATGGGA ATGGAGGAGC CTCTCCTCTT 
GAATTGCGTG AGAGTGGTAA CAGTCCGTGT TTAAGTCGAT GCAGCAGCAC ACATAGCAAA 
CGGCGGAAGC ATCCACTGGG GGACAATCCA AACGGCAGTT CGATAGGCAA TGCCAACGGG 
GAGTCGCATG AGCAGGACAG CTGGTCGGAC GAGGAGGGCG AGACCACGGA CTACAAGTAC 
GCTCTGCGGA GAAGAAGCAT TGGCCGCCAG CCCATAGCTC GTGGCATGCG ACCCCGTCGC 
AGCTACAAGG CTCCACTCTC CCAGAAGATT GCCATCCACA AGCGCAATGT GGTAATCGTA 
TCAGACGAGG AGGAAAACAC CTCCGAGTAC AGTCACTCAC CCTCCAGCCA GCACTCGACA 
CTAGAGAGCA ATACCGACAT TGCGGACATG AAGAAAACTC AGGCCACATC CACCTCAAGC 
AACAGCTATA GCGAGCCCGA AGACGATTCG AGTGATTCCA GCGATGAGGA GCAGGGAAAC 
CGGCCGACGG AAGCCAAGGC CGAGGGTCCA GCTTTGCCTA TGGGCGCCGT ACGCAGCAGC 
GATATTATAT CCATACCTAC TTTTGAGGCT GATGGCGCCG TCAATATGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnd ( NM_176363.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788541.1 Drosophila melanogaster wallenda (wnd), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18994
Clone ID Related Accession (Same CDS sequence)	NM_176362.2 , NM_176363.3 , NM_140880.3
Accession Version	NM_176363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wallenda, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_176363.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCGT TCAGCGACAG TCTGAGCAAA AGTCGCGATG ATCTGGTCAC CGCCGCCTCC 
CGGCAACAAC AACAGCTACA CGGACGGAGG CGACACCACG GCAGCAGCCC CAACTTGTCG 
CTCGATCAGA CCGACAATCT GCGAAGGAGC ATGGCTTGCC TGCAGGATGA GTTCGGTCAC 
CTAGGCATCG CCGCTACAGA CCTGCCGTTC AAATCCTCCG ACCTGGACTC TCCGCCCCGC 
CTGCAGCATC ACAACAACTA TGCGGAGATC ACGGACAGCA GTGCGGAAAA CACGTGCCAA 
CAGCGCTGGC CACCTCATGT GGGAGGAGCA GGGGCGGCCT TCGGGCATCC GGACAAGCCC 
ATTGGCTGGA TGTACGGACT GCTCGGATGC ATGAAGCCCG TGCTTTCGTT CATCGGGAAG 
ACGGGGGTGA TCGAGGTGAA GAGTCAGCGG AGCGAGGACT GGCAGATTCC ATTTGAGTCC 
ATCACCGAGT TGGAATGGCT GGGAAGCGGA GCACAAGGAG CCGTCTTCAG TGGCAGGCTA 
AAGAACGAGA CTGTGGCGGT CAAGAAGGTG AAGGAGCTGA AGGAAACGGA CATCAAGCAT 
CTCAGGAAGC TCGATCATGA GAACATTATT AAGTTCAAAG GGGTTTGCAC GCAGTCACCG 
GTGTTCTGCA TCATCATGGA GTTCTGTCCG TATGGCCCAC TGCAGAATAT CCTGAAGGAG 
GAGCAAGTCA TGTTACCGTC CCGCTTGGTC TCTTGGTCCA AGCAAATTGC CCTGGGCATG 
CAGTATCTGC ATTCCCACAA GATCATACAC CGAGATCTAA AGAGTCCAAA CATACTGATT 
AGTACAAATG AGGTGGTGAA GATCAGCGAC TTTGGCACCA GTCGCGAGTG GAACGAGATC 
AGCACCAAGA TGAGCTTTGC TGGTACCGTA GCCTGGATGG CTCCGGAAGT GATCAGGAAT 
GAGCCGTGCT CCGAGAAGGT GGACATTTGG TCGTACGGTG TGGTGCTATG GGAAATGCTC 
ACCTGCGAGA TACCTTACAA GGATGTGGAC TCATCAGCCA TAATCTGGGG TGTGGGCAAC 
AACTCTCTCA AGCTGCTGGT CCCGAGCACC TGTCCGGAGG GATTTAAGTT GCTGGTTAAG 
CTCTGTTGGA AAAGTAAGCC CCGAAATCGG CCATCGTTCC GGCAGATACT CTCGCACTTG 
GATATCGCCG GACCGGAACT GCTGAGAAAA ACCGAGAAGC AATATTTTGA GACGCAAAAG 
TCGTGGAAGG AGGAGGTGAG ATCTCACTTG AAGGAGATTA CCCAAAATGG CACCAACATA 
CACAAGTACG AGCAAGATCT GATTAAGCGG CGGACGGCAG AGTGGCGACA TGCCCAGGAT 
ATCCGAATGG TGTACGAGGA TAAGTTGCAA AAGACCAACC AGCTTTTCTT CGAACTCAGC 
GAGTGCATGT CCCAGTTGCA GGAAAAGGAG AAGGAAATCG CAGAACGAGA ACGGAAACTA 
CCAGGAAGTG GCTACAAACC CAATCGACGT TTTGGCAACA CGATCCGAAA AATGCAACAC 
TATCGCCGAC GCCTGAACCC TGCTCCTGCC GCCATTCAGC AACAATCAAC AACGCCGGAT 
CCAGAGACTA CGCCGGAGTC CCCTGTGAAG TGTATGCTAT ATGCCCAGCT TGATAGCAAT 
TGCCAACCCA AATCGTATCT GGCCAATATC ATCCCGAGTA GCGGCTTAGG CGCACCCATG 
CCCAATAAAA ACAAGAAGGT CTTCCGGCAC CGACGCAACG CATCTGGTTC CTTCGGTGCA 
CCACCCAAGT ACAGTCCGAC GAGGGATCGT CGTTACCAGA GCGAACCGGA GAACCGAAAG 
GTGCAGCTGG TGGAGAGACA AACCCAAACA GATGCCATGG ACGTGAGTGA AACGGATATT 
AGTCCTAGCG CGGAGGCTCC TCGATCTCAG CCCATCGACG TGCCAGTTCC GAATCATCGC 
CAACTGCCGC TGCAACTGCA GAGAGTACAG AAAATTGCCC AAGCTCAGGC GAGGGCTCGA 
AGTGGAAGCA CTTCATCTGC AGCTGGCGCC GTGAATCCCG CTTGTCCGTC GAATGGCAAC 
TCTCTGAGCA CAAGCGAGCT GACCTACCAA GATGCCTGCT CTAGTCCCGA TCAGCTCATC 
GACGATGTAA TGAACAGCAA CGAACGTTTG GACATGACTG AATGCTGCAG TGACAACGAA 
AACCTGGAAC GTCTAGGCCG CAAAGTCATC GAATTCATCA ACGAAAACCG CCTGTCCATT 
CAGTCAAACA CGAACTCCGT GAGCAATGCC GATAATGGGA ATGGAGGAGC CTCTCCTCTT 
GAATTGCGTG AGAGTGGTAA CAGTCCGTGT TTAAGTCGAT GCAGCAGCAC ACATAGCAAA 
CGGCGGAAGC ATCCACTGGG GGACAATCCA AACGGCAGTT CGATAGGCAA TGCCAACGGG 
GAGTCGCATG AGCAGGACAG CTGGTCGGAC GAGGAGGGCG AGACCACGGA CTACAAGTAC 
GCTCTGCGGA GAAGAAGCAT TGGCCGCCAG CCCATAGCTC GTGGCATGCG ACCCCGTCGC 
AGCTACAAGG CTCCACTCTC CCAGAAGATT GCCATCCACA AGCGCAATGT GGTAATCGTA 
TCAGACGAGG AGGAAAACAC CTCCGAGTAC AGTCACTCAC CCTCCAGCCA GCACTCGACA 
CTAGAGAGCA ATACCGACAT TGCGGACATG AAGAAAACTC AGGCCACATC CACCTCAAGC 
AACAGCTATA GCGAGCCCGA AGACGATTCG AGTGATTCCA GCGATGAGGA GCAGGGAAAC 
CGGCCGACGG AAGCCAAGGC CGAGGGTCCA GCTTTGCCTA TGGGCGCCGT ACGCAGCAGC 
GATATTATAT CCATACCTAC TTTTGAGGCT GATGGCGCCG TCAATATGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788541.1
CDS	437..3370
Translation	MQPFSDSLSKSRDDLVTAASRQQQQLHGRRRHHGSSPNLSLDQTDNLRRSMACLQDEFGHLGIAATDLPFKSSDLDSPPRLQHHNNYAEITDSSAENTCQQRWPPHVGGAGAAFGHPDKPIGWMYGLLGCMKPVLSFIGKTGVIEVKSQRSEDWQIPFESITELEWLGSGAQGAVFSGRLKNETVAVKKVKELKETDIKHLRKLDHENIIKFKGVCTQSPVFCIIMEFCPYGPLQNILKEEQVMLPSRLVSWSKQIALGMQYLHSHKIIHRDLKSPNILISTNEVVKISDFGTSREWNEISTKMSFAGTVAWMAPEVIRNEPCSEKVDIWSYGVVLWEMLTCEIPYKDVDSSAIIWGVGNNSLKLLVPSTCPEGFKLLVKLCWKSKPRNRPSFRQILSHLDIAGPELLRKTEKQYFETQKSWKEEVRSHLKEITQNGTNIHKYEQDLIKRRTAEWRHAQDIRMVYEDKLQKTNQLFFELSECMSQLQEKEKEIAERERKLPGSGYKPNRRFGNTIRKMQHYRRRLNPAPAAIQQQSTTPDPETTPESPVKCMLYAQLDSNCQPKSYLANIIPSSGLGAPMPNKNKKVFRHRRNASGSFGAPPKYSPTRDRRYQSEPENRKVQLVERQTQTDAMDVSETDISPSAEAPRSQPIDVPVPNHRQLPLQLQRVQKIAQAQARARSGSTSSAAGAVNPACPSNGNSLSTSELTYQDACSSPDQLIDDVMNSNERLDMTECCSDNENLERLGRKVIEFINENRLSIQSNTNSVSNADNGNGGASPLELRESGNSPCLSRCSSTHSKRRKHPLGDNPNGSSIGNANGESHEQDSWSDEEGETTDYKYALRRRSIGRQPIARGMRPRRSYKAPLSQKIAIHKRNVVIVSDEEENTSEYSHSPSSQHSTLESNTDIADMKKTQATSTSSNSYSEPEDDSSDSSDEEQGNRPTEAKAEGPALPMGAVRSSDIISIPTFEADGAVNMV

Target ORF information:

RefSeq Version	NM_176363.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wallenda (wnd), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176363.3

ORF Insert Sequence:

ATGCAACCGT TCAGCGACAG TCTGAGCAAA AGTCGCGATG ATCTGGTCAC CGCCGCCTCC 
CGGCAACAAC AACAGCTACA CGGACGGAGG CGACACCACG GCAGCAGCCC CAACTTGTCG 
CTCGATCAGA CCGACAATCT GCGAAGGAGC ATGGCTTGCC TGCAGGATGA GTTCGGTCAC 
CTAGGCATCG CCGCTACAGA CCTGCCGTTC AAATCCTCCG ACCTGGACTC TCCGCCCCGC 
CTGCAGCATC ACAACAACTA TGCGGAGATC ACGGACAGCA GTGCGGAAAA CACGTGCCAA 
CAGCGCTGGC CACCTCATGT GGGAGGAGCA GGGGCGGCCT TCGGGCATCC GGACAAGCCC 
ATTGGCTGGA TGTACGGACT GCTCGGATGC ATGAAGCCCG TGCTTTCGTT CATCGGGAAG 
ACGGGGGTGA TCGAGGTGAA GAGTCAGCGG AGCGAGGACT GGCAGATTCC ATTTGAGTCC 
ATCACCGAGT TGGAATGGCT GGGAAGCGGA GCACAAGGAG CCGTCTTCAG TGGCAGGCTA 
AAGAACGAGA CTGTGGCGGT CAAGAAGGTG AAGGAGCTGA AGGAAACGGA CATCAAGCAT 
CTCAGGAAGC TCGATCATGA GAACATTATT AAGTTCAAAG GGGTTTGCAC GCAGTCACCG 
GTGTTCTGCA TCATCATGGA GTTCTGTCCG TATGGCCCAC TGCAGAATAT CCTGAAGGAG 
GAGCAAGTCA TGTTACCGTC CCGCTTGGTC TCTTGGTCCA AGCAAATTGC CCTGGGCATG 
CAGTATCTGC ATTCCCACAA GATCATACAC CGAGATCTAA AGAGTCCAAA CATACTGATT 
AGTACAAATG AGGTGGTGAA GATCAGCGAC TTTGGCACCA GTCGCGAGTG GAACGAGATC 
AGCACCAAGA TGAGCTTTGC TGGTACCGTA GCCTGGATGG CTCCGGAAGT GATCAGGAAT 
GAGCCGTGCT CCGAGAAGGT GGACATTTGG TCGTACGGTG TGGTGCTATG GGAAATGCTC 
ACCTGCGAGA TACCTTACAA GGATGTGGAC TCATCAGCCA TAATCTGGGG TGTGGGCAAC 
AACTCTCTCA AGCTGCTGGT CCCGAGCACC TGTCCGGAGG GATTTAAGTT GCTGGTTAAG 
CTCTGTTGGA AAAGTAAGCC CCGAAATCGG CCATCGTTCC GGCAGATACT CTCGCACTTG 
GATATCGCCG GACCGGAACT GCTGAGAAAA ACCGAGAAGC AATATTTTGA GACGCAAAAG 
TCGTGGAAGG AGGAGGTGAG ATCTCACTTG AAGGAGATTA CCCAAAATGG CACCAACATA 
CACAAGTACG AGCAAGATCT GATTAAGCGG CGGACGGCAG AGTGGCGACA TGCCCAGGAT 
ATCCGAATGG TGTACGAGGA TAAGTTGCAA AAGACCAACC AGCTTTTCTT CGAACTCAGC 
GAGTGCATGT CCCAGTTGCA GGAAAAGGAG AAGGAAATCG CAGAACGAGA ACGGAAACTA 
CCAGGAAGTG GCTACAAACC CAATCGACGT TTTGGCAACA CGATCCGAAA AATGCAACAC 
TATCGCCGAC GCCTGAACCC TGCTCCTGCC GCCATTCAGC AACAATCAAC AACGCCGGAT 
CCAGAGACTA CGCCGGAGTC CCCTGTGAAG TGTATGCTAT ATGCCCAGCT TGATAGCAAT 
TGCCAACCCA AATCGTATCT GGCCAATATC ATCCCGAGTA GCGGCTTAGG CGCACCCATG 
CCCAATAAAA ACAAGAAGGT CTTCCGGCAC CGACGCAACG CATCTGGTTC CTTCGGTGCA 
CCACCCAAGT ACAGTCCGAC GAGGGATCGT CGTTACCAGA GCGAACCGGA GAACCGAAAG 
GTGCAGCTGG TGGAGAGACA AACCCAAACA GATGCCATGG ACGTGAGTGA AACGGATATT 
AGTCCTAGCG CGGAGGCTCC TCGATCTCAG CCCATCGACG TGCCAGTTCC GAATCATCGC 
CAACTGCCGC TGCAACTGCA GAGAGTACAG AAAATTGCCC AAGCTCAGGC GAGGGCTCGA 
AGTGGAAGCA CTTCATCTGC AGCTGGCGCC GTGAATCCCG CTTGTCCGTC GAATGGCAAC 
TCTCTGAGCA CAAGCGAGCT GACCTACCAA GATGCCTGCT CTAGTCCCGA TCAGCTCATC 
GACGATGTAA TGAACAGCAA CGAACGTTTG GACATGACTG AATGCTGCAG TGACAACGAA 
AACCTGGAAC GTCTAGGCCG CAAAGTCATC GAATTCATCA ACGAAAACCG CCTGTCCATT 
CAGTCAAACA CGAACTCCGT GAGCAATGCC GATAATGGGA ATGGAGGAGC CTCTCCTCTT 
GAATTGCGTG AGAGTGGTAA CAGTCCGTGT TTAAGTCGAT GCAGCAGCAC ACATAGCAAA 
CGGCGGAAGC ATCCACTGGG GGACAATCCA AACGGCAGTT CGATAGGCAA TGCCAACGGG 
GAGTCGCATG AGCAGGACAG CTGGTCGGAC GAGGAGGGCG AGACCACGGA CTACAAGTAC 
GCTCTGCGGA GAAGAAGCAT TGGCCGCCAG CCCATAGCTC GTGGCATGCG ACCCCGTCGC 
AGCTACAAGG CTCCACTCTC CCAGAAGATT GCCATCCACA AGCGCAATGT GGTAATCGTA 
TCAGACGAGG AGGAAAACAC CTCCGAGTAC AGTCACTCAC CCTCCAGCCA GCACTCGACA 
CTAGAGAGCA ATACCGACAT TGCGGACATG AAGAAAACTC AGGCCACATC CACCTCAAGC 
AACAGCTATA GCGAGCCCGA AGACGATTCG AGTGATTCCA GCGATGAGGA GCAGGGAAAC 
CGGCCGACGG AAGCCAAGGC CGAGGGTCCA GCTTTGCCTA TGGGCGCCGT ACGCAGCAGC 
GATATTATAT CCATACCTAC TTTTGAGGCT GATGGCGCCG TCAATATGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnd ( NM_140880.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649137.3 Drosophila melanogaster wallenda (wnd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18994
Clone ID Related Accession (Same CDS sequence)	NM_176362.2 , NM_176363.3 , NM_140880.3
Accession Version	NM_140880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wallenda, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_140880.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACCGT TCAGCGACAG TCTGAGCAAA AGTCGCGATG ATCTGGTCAC CGCCGCCTCC 
CGGCAACAAC AACAGCTACA CGGACGGAGG CGACACCACG GCAGCAGCCC CAACTTGTCG 
CTCGATCAGA CCGACAATCT GCGAAGGAGC ATGGCTTGCC TGCAGGATGA GTTCGGTCAC 
CTAGGCATCG CCGCTACAGA CCTGCCGTTC AAATCCTCCG ACCTGGACTC TCCGCCCCGC 
CTGCAGCATC ACAACAACTA TGCGGAGATC ACGGACAGCA GTGCGGAAAA CACGTGCCAA 
CAGCGCTGGC CACCTCATGT GGGAGGAGCA GGGGCGGCCT TCGGGCATCC GGACAAGCCC 
ATTGGCTGGA TGTACGGACT GCTCGGATGC ATGAAGCCCG TGCTTTCGTT CATCGGGAAG 
ACGGGGGTGA TCGAGGTGAA GAGTCAGCGG AGCGAGGACT GGCAGATTCC ATTTGAGTCC 
ATCACCGAGT TGGAATGGCT GGGAAGCGGA GCACAAGGAG CCGTCTTCAG TGGCAGGCTA 
AAGAACGAGA CTGTGGCGGT CAAGAAGGTG AAGGAGCTGA AGGAAACGGA CATCAAGCAT 
CTCAGGAAGC TCGATCATGA GAACATTATT AAGTTCAAAG GGGTTTGCAC GCAGTCACCG 
GTGTTCTGCA TCATCATGGA GTTCTGTCCG TATGGCCCAC TGCAGAATAT CCTGAAGGAG 
GAGCAAGTCA TGTTACCGTC CCGCTTGGTC TCTTGGTCCA AGCAAATTGC CCTGGGCATG 
CAGTATCTGC ATTCCCACAA GATCATACAC CGAGATCTAA AGAGTCCAAA CATACTGATT 
AGTACAAATG AGGTGGTGAA GATCAGCGAC TTTGGCACCA GTCGCGAGTG GAACGAGATC 
AGCACCAAGA TGAGCTTTGC TGGTACCGTA GCCTGGATGG CTCCGGAAGT GATCAGGAAT 
GAGCCGTGCT CCGAGAAGGT GGACATTTGG TCGTACGGTG TGGTGCTATG GGAAATGCTC 
ACCTGCGAGA TACCTTACAA GGATGTGGAC TCATCAGCCA TAATCTGGGG TGTGGGCAAC 
AACTCTCTCA AGCTGCTGGT CCCGAGCACC TGTCCGGAGG GATTTAAGTT GCTGGTTAAG 
CTCTGTTGGA AAAGTAAGCC CCGAAATCGG CCATCGTTCC GGCAGATACT CTCGCACTTG 
GATATCGCCG GACCGGAACT GCTGAGAAAA ACCGAGAAGC AATATTTTGA GACGCAAAAG 
TCGTGGAAGG AGGAGGTGAG ATCTCACTTG AAGGAGATTA CCCAAAATGG CACCAACATA 
CACAAGTACG AGCAAGATCT GATTAAGCGG CGGACGGCAG AGTGGCGACA TGCCCAGGAT 
ATCCGAATGG TGTACGAGGA TAAGTTGCAA AAGACCAACC AGCTTTTCTT CGAACTCAGC 
GAGTGCATGT CCCAGTTGCA GGAAAAGGAG AAGGAAATCG CAGAACGAGA ACGGAAACTA 
CCAGGAAGTG GCTACAAACC CAATCGACGT TTTGGCAACA CGATCCGAAA AATGCAACAC 
TATCGCCGAC GCCTGAACCC TGCTCCTGCC GCCATTCAGC AACAATCAAC AACGCCGGAT 
CCAGAGACTA CGCCGGAGTC CCCTGTGAAG TGTATGCTAT ATGCCCAGCT TGATAGCAAT 
TGCCAACCCA AATCGTATCT GGCCAATATC ATCCCGAGTA GCGGCTTAGG CGCACCCATG 
CCCAATAAAA ACAAGAAGGT CTTCCGGCAC CGACGCAACG CATCTGGTTC CTTCGGTGCA 
CCACCCAAGT ACAGTCCGAC GAGGGATCGT CGTTACCAGA GCGAACCGGA GAACCGAAAG 
GTGCAGCTGG TGGAGAGACA AACCCAAACA GATGCCATGG ACGTGAGTGA AACGGATATT 
AGTCCTAGCG CGGAGGCTCC TCGATCTCAG CCCATCGACG TGCCAGTTCC GAATCATCGC 
CAACTGCCGC TGCAACTGCA GAGAGTACAG AAAATTGCCC AAGCTCAGGC GAGGGCTCGA 
AGTGGAAGCA CTTCATCTGC AGCTGGCGCC GTGAATCCCG CTTGTCCGTC GAATGGCAAC 
TCTCTGAGCA CAAGCGAGCT GACCTACCAA GATGCCTGCT CTAGTCCCGA TCAGCTCATC 
GACGATGTAA TGAACAGCAA CGAACGTTTG GACATGACTG AATGCTGCAG TGACAACGAA 
AACCTGGAAC GTCTAGGCCG CAAAGTCATC GAATTCATCA ACGAAAACCG CCTGTCCATT 
CAGTCAAACA CGAACTCCGT GAGCAATGCC GATAATGGGA ATGGAGGAGC CTCTCCTCTT 
GAATTGCGTG AGAGTGGTAA CAGTCCGTGT TTAAGTCGAT GCAGCAGCAC ACATAGCAAA 
CGGCGGAAGC ATCCACTGGG GGACAATCCA AACGGCAGTT CGATAGGCAA TGCCAACGGG 
GAGTCGCATG AGCAGGACAG CTGGTCGGAC GAGGAGGGCG AGACCACGGA CTACAAGTAC 
GCTCTGCGGA GAAGAAGCAT TGGCCGCCAG CCCATAGCTC GTGGCATGCG ACCCCGTCGC 
AGCTACAAGG CTCCACTCTC CCAGAAGATT GCCATCCACA AGCGCAATGT GGTAATCGTA 
TCAGACGAGG AGGAAAACAC CTCCGAGTAC AGTCACTCAC CCTCCAGCCA GCACTCGACA 
CTAGAGAGCA ATACCGACAT TGCGGACATG AAGAAAACTC AGGCCACATC CACCTCAAGC 
AACAGCTATA GCGAGCCCGA AGACGATTCG AGTGATTCCA GCGATGAGGA GCAGGGAAAC 
CGGCCGACGG AAGCCAAGGC CGAGGGTCCA GCTTTGCCTA TGGGCGCCGT ACGCAGCAGC 
GATATTATAT CCATACCTAC TTTTGAGGCT GATGGCGCCG TCAATATGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649137.3
CDS	309..3242
Translation	MQPFSDSLSKSRDDLVTAASRQQQQLHGRRRHHGSSPNLSLDQTDNLRRSMACLQDEFGHLGIAATDLPFKSSDLDSPPRLQHHNNYAEITDSSAENTCQQRWPPHVGGAGAAFGHPDKPIGWMYGLLGCMKPVLSFIGKTGVIEVKSQRSEDWQIPFESITELEWLGSGAQGAVFSGRLKNETVAVKKVKELKETDIKHLRKLDHENIIKFKGVCTQSPVFCIIMEFCPYGPLQNILKEEQVMLPSRLVSWSKQIALGMQYLHSHKIIHRDLKSPNILISTNEVVKISDFGTSREWNEISTKMSFAGTVAWMAPEVIRNEPCSEKVDIWSYGVVLWEMLTCEIPYKDVDSSAIIWGVGNNSLKLLVPSTCPEGFKLLVKLCWKSKPRNRPSFRQILSHLDIAGPELLRKTEKQYFETQKSWKEEVRSHLKEITQNGTNIHKYEQDLIKRRTAEWRHAQDIRMVYEDKLQKTNQLFFELSECMSQLQEKEKEIAERERKLPGSGYKPNRRFGNTIRKMQHYRRRLNPAPAAIQQQSTTPDPETTPESPVKCMLYAQLDSNCQPKSYLANIIPSSGLGAPMPNKNKKVFRHRRNASGSFGAPPKYSPTRDRRYQSEPENRKVQLVERQTQTDAMDVSETDISPSAEAPRSQPIDVPVPNHRQLPLQLQRVQKIAQAQARARSGSTSSAAGAVNPACPSNGNSLSTSELTYQDACSSPDQLIDDVMNSNERLDMTECCSDNENLERLGRKVIEFINENRLSIQSNTNSVSNADNGNGGASPLELRESGNSPCLSRCSSTHSKRRKHPLGDNPNGSSIGNANGESHEQDSWSDEEGETTDYKYALRRRSIGRQPIARGMRPRRSYKAPLSQKIAIHKRNVVIVSDEEENTSEYSHSPSSQHSTLESNTDIADMKKTQATSTSSNSYSEPEDDSSDSSDEEQGNRPTEAKAEGPALPMGAVRSSDIISIPTFEADGAVNMV

Target ORF information:

RefSeq Version	NM_140880.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wallenda (wnd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140880.3

ORF Insert Sequence:

ATGCAACCGT TCAGCGACAG TCTGAGCAAA AGTCGCGATG ATCTGGTCAC CGCCGCCTCC 
CGGCAACAAC AACAGCTACA CGGACGGAGG CGACACCACG GCAGCAGCCC CAACTTGTCG 
CTCGATCAGA CCGACAATCT GCGAAGGAGC ATGGCTTGCC TGCAGGATGA GTTCGGTCAC 
CTAGGCATCG CCGCTACAGA CCTGCCGTTC AAATCCTCCG ACCTGGACTC TCCGCCCCGC 
CTGCAGCATC ACAACAACTA TGCGGAGATC ACGGACAGCA GTGCGGAAAA CACGTGCCAA 
CAGCGCTGGC CACCTCATGT GGGAGGAGCA GGGGCGGCCT TCGGGCATCC GGACAAGCCC 
ATTGGCTGGA TGTACGGACT GCTCGGATGC ATGAAGCCCG TGCTTTCGTT CATCGGGAAG 
ACGGGGGTGA TCGAGGTGAA GAGTCAGCGG AGCGAGGACT GGCAGATTCC ATTTGAGTCC 
ATCACCGAGT TGGAATGGCT GGGAAGCGGA GCACAAGGAG CCGTCTTCAG TGGCAGGCTA 
AAGAACGAGA CTGTGGCGGT CAAGAAGGTG AAGGAGCTGA AGGAAACGGA CATCAAGCAT 
CTCAGGAAGC TCGATCATGA GAACATTATT AAGTTCAAAG GGGTTTGCAC GCAGTCACCG 
GTGTTCTGCA TCATCATGGA GTTCTGTCCG TATGGCCCAC TGCAGAATAT CCTGAAGGAG 
GAGCAAGTCA TGTTACCGTC CCGCTTGGTC TCTTGGTCCA AGCAAATTGC CCTGGGCATG 
CAGTATCTGC ATTCCCACAA GATCATACAC CGAGATCTAA AGAGTCCAAA CATACTGATT 
AGTACAAATG AGGTGGTGAA GATCAGCGAC TTTGGCACCA GTCGCGAGTG GAACGAGATC 
AGCACCAAGA TGAGCTTTGC TGGTACCGTA GCCTGGATGG CTCCGGAAGT GATCAGGAAT 
GAGCCGTGCT CCGAGAAGGT GGACATTTGG TCGTACGGTG TGGTGCTATG GGAAATGCTC 
ACCTGCGAGA TACCTTACAA GGATGTGGAC TCATCAGCCA TAATCTGGGG TGTGGGCAAC 
AACTCTCTCA AGCTGCTGGT CCCGAGCACC TGTCCGGAGG GATTTAAGTT GCTGGTTAAG 
CTCTGTTGGA AAAGTAAGCC CCGAAATCGG CCATCGTTCC GGCAGATACT CTCGCACTTG 
GATATCGCCG GACCGGAACT GCTGAGAAAA ACCGAGAAGC AATATTTTGA GACGCAAAAG 
TCGTGGAAGG AGGAGGTGAG ATCTCACTTG AAGGAGATTA CCCAAAATGG CACCAACATA 
CACAAGTACG AGCAAGATCT GATTAAGCGG CGGACGGCAG AGTGGCGACA TGCCCAGGAT 
ATCCGAATGG TGTACGAGGA TAAGTTGCAA AAGACCAACC AGCTTTTCTT CGAACTCAGC 
GAGTGCATGT CCCAGTTGCA GGAAAAGGAG AAGGAAATCG CAGAACGAGA ACGGAAACTA 
CCAGGAAGTG GCTACAAACC CAATCGACGT TTTGGCAACA CGATCCGAAA AATGCAACAC 
TATCGCCGAC GCCTGAACCC TGCTCCTGCC GCCATTCAGC AACAATCAAC AACGCCGGAT 
CCAGAGACTA CGCCGGAGTC CCCTGTGAAG TGTATGCTAT ATGCCCAGCT TGATAGCAAT 
TGCCAACCCA AATCGTATCT GGCCAATATC ATCCCGAGTA GCGGCTTAGG CGCACCCATG 
CCCAATAAAA ACAAGAAGGT CTTCCGGCAC CGACGCAACG CATCTGGTTC CTTCGGTGCA 
CCACCCAAGT ACAGTCCGAC GAGGGATCGT CGTTACCAGA GCGAACCGGA GAACCGAAAG 
GTGCAGCTGG TGGAGAGACA AACCCAAACA GATGCCATGG ACGTGAGTGA AACGGATATT 
AGTCCTAGCG CGGAGGCTCC TCGATCTCAG CCCATCGACG TGCCAGTTCC GAATCATCGC 
CAACTGCCGC TGCAACTGCA GAGAGTACAG AAAATTGCCC AAGCTCAGGC GAGGGCTCGA 
AGTGGAAGCA CTTCATCTGC AGCTGGCGCC GTGAATCCCG CTTGTCCGTC GAATGGCAAC 
TCTCTGAGCA CAAGCGAGCT GACCTACCAA GATGCCTGCT CTAGTCCCGA TCAGCTCATC 
GACGATGTAA TGAACAGCAA CGAACGTTTG GACATGACTG AATGCTGCAG TGACAACGAA 
AACCTGGAAC GTCTAGGCCG CAAAGTCATC GAATTCATCA ACGAAAACCG CCTGTCCATT 
CAGTCAAACA CGAACTCCGT GAGCAATGCC GATAATGGGA ATGGAGGAGC CTCTCCTCTT 
GAATTGCGTG AGAGTGGTAA CAGTCCGTGT TTAAGTCGAT GCAGCAGCAC ACATAGCAAA 
CGGCGGAAGC ATCCACTGGG GGACAATCCA AACGGCAGTT CGATAGGCAA TGCCAACGGG 
GAGTCGCATG AGCAGGACAG CTGGTCGGAC GAGGAGGGCG AGACCACGGA CTACAAGTAC 
GCTCTGCGGA GAAGAAGCAT TGGCCGCCAG CCCATAGCTC GTGGCATGCG ACCCCGTCGC 
AGCTACAAGG CTCCACTCTC CCAGAAGATT GCCATCCACA AGCGCAATGT GGTAATCGTA 
TCAGACGAGG AGGAAAACAC CTCCGAGTAC AGTCACTCAC CCTCCAGCCA GCACTCGACA 
CTAGAGAGCA ATACCGACAT TGCGGACATG AAGAAAACTC AGGCCACATC CACCTCAAGC 
AACAGCTATA GCGAGCCCGA AGACGATTCG AGTGATTCCA GCGATGAGGA GCAGGGAAAC 
CGGCCGACGG AAGCCAAGGC CGAGGGTCCA GCTTTGCCTA TGGGCGCCGT ACGCAGCAGC 
GATATTATAT CCATACCTAC TTTTGAGGCT GATGGCGCCG TCAATATGGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnd ( NM_001202203.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189132.1 Drosophila melanogaster wallenda (wnd), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21089
Clone ID Related Accession (Same CDS sequence)	NM_001202203.1
Accession Version	NM_001202203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wallenda, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCTACA TCATACCGTT CTTTAGCCAC AATGACCCGC CCGACATGAT CATCACCAAA 
GAAAAAAGCA TGGCTTGCCT GCAGGATGAG TTCGGTCACC TAGGCATCGC CGCTACAGAC 
CTGCCGTTCA AATCCTCCGA CCTGGACTCT CCGCCCCGCC TGCAGCATCA CAACAACTAT 
GCGGAGATCA CGGACAGCAG TGCGGAAAAC ACGTGCCAAC AGCGCTGGCC ACCTCATGTG 
GGAGGAGCAG GGGCGGCCTT CGGGCATCCG GACAAGCCCA TTGGCTGGAT GTACGGACTG 
CTCGGATGCA TGAAGCCCGT GCTTTCGTTC ATCGGGAAGA CGGGGGTGAT CGAGGTGAAG 
AGTCAGCGGA GCGAGGACTG GCAGATTCCA TTTGAGTCCA TCACCGAGTT GGAATGGCTG 
GGAAGCGGAG CACAAGGAGC CGTCTTCAGT GGCAGGCTAA AGAACGAGAC TGTGGCGGTC 
AAGAAGGTGA AGGAGCTGAA GGAAACGGAC ATCAAGCATC TCAGGAAGCT CGATCATGAG 
AACATTATTA AGTTCAAAGG GGTTTGCACG CAGTCACCGG TGTTCTGCAT CATCATGGAG 
TTCTGTCCGT ATGGCCCACT GCAGAATATC CTGAAGGAGG AGCAAGTCAT GTTACCGTCC 
CGCTTGGTCT CTTGGTCCAA GCAAATTGCC CTGGGCATGC AGTATCTGCA TTCCCACAAG 
ATCATACACC GAGATCTAAA GAGTCCAAAC ATACTGATTA GTACAAATGA GGTGGTGAAG 
ATCAGCGACT TTGGCACCAG TCGCGAGTGG AACGAGATCA GCACCAAGAT GAGCTTTGCT 
GGTACCGTAG CCTGGATGGC TCCGGAAGTG ATCAGGAATG AGCCGTGCTC CGAGAAGGTG 
GACATTTGGT CGTACGGTGT GGTGCTATGG GAAATGCTCA CCTGCGAGAT ACCTTACAAG 
GATGTGGACT CATCAGCCAT AATCTGGGGT GTGGGCAACA ACTCTCTCAA GCTGCTGGTC 
CCGAGCACCT GTCCGGAGGG ATTTAAGTTG CTGGTTAAGC TCTGTTGGAA AAGTAAGCCC 
CGAAATCGGC CATCGTTCCG GCAGATACTC TCGCACTTGG ATATCGCCGG ACCGGAACTG 
CTGAGAAAAA CCGAGAAGCA ATATTTTGAG ACGCAAAAGT CGTGGAAGGA GGAGGTGAGA 
TCTCACTTGA AGGAGATTAC CCAAAATGGC ACCAACATAC ACAAGTACGA GCAAGATCTG 
ATTAAGCGGC GGACGGCAGA GTGGCGACAT GCCCAGGATA TCCGAATGGT GTACGAGGAT 
AAGTTGCAAA AGACCAACCA GCTTTTCTTC GAACTCAGCG AGTGCATGTC CCAGTTGCAG 
GAAAAGGAGA AGGAAATCGC AGAACGAGAA CGGAAACTAC CAGGAAGTGG CTACAAACCC 
AATCGACGTT TTGGCAACAC GATCCGAAAA ATGCAACACT ATCGCCGACG CCTGAACCCT 
GCTCCTGCCG CCATTCAGCA ACAATCAACA ACGCCGGATC CAGAGACTAC GCCGGAGTCC 
CCTGTGAAGT GTATGCTATA TGCCCAGCTT GATAGCAATT GCCAACCCAA ATCGTATCTG 
GCCAATATCA TCCCGAGTAG CGGCTTAGGC GCACCCATGC CCAATAAAAA CAAGAAGGTC 
TTCCGGCACC GACGCAACGC ATCTGGTTCC TTCGGTGCAC CACCCAAGTA CAGTCCGACG 
AGGGATCGTC GTTACCAGAG CGAACCGGAG AACCGAAAGG TGCAGCTGGT GGAGAGACAA 
ACCCAAACAG ATGCCATGGA CGTGAGTGAA ACGGATATTA GTCCTAGCGC GGAGGCTCCT 
CGATCTCAGC CCATCGACGT GCCAGTTCCG AATCATCGCC AACTGCCGCT GCAACTGCAG 
AGAGTACAGA AAATTGCCCA AGCTCAGGCG AGGGCTCGAA GTGGAAGCAC TTCATCTGCA 
GCTGGCGCCG TGAATCCCGC TTGTCCGTCG AATGGCAACT CTCTGAGCAC AAGCGAGCTG 
ACCTACCAAG ATGCCTGCTC TAGTCCCGAT CAGCTCATCG ACGATGTAAT GAACAGCAAC 
GAACGTTTGG ACATGACTGA ATGCTGCAGT GACAACGAAA ACCTGGAACG TCTAGGCCGC 
AAAGTCATCG AATTCATCAA CGAAAACCGC CTGTCCATTC AGTCAAACAC GAACTCCGTG 
AGCAATGCCG ATAATGGGAA TGGAGGAGCC TCTCCTCTTG AATTGCGTGA GAGTGGTAAC 
AGTCCGTGTT TAAGTCGATG CAGCAGCACA CATAGCAAAC GGCGGAAGCA TCCACTGGGG 
GACAATCCAA ACGGCAGTTC GATAGGCAAT GCCAACGGGG AGTCGCATGA GCAGGACAGC 
TGGTCGGACG AGGAGGGCGA GACCACGGAC TACAAGTACG CTCTGCGGAG AAGAAGCATT 
GGCCGCCAGC CCATAGCTCG TGGCATGCGA CCCCGTCGCA GCTACAAGGC TCCACTCTCC 
CAGAAGATTG CCATCCACAA GCGCAATGTG GTAATCGTAT CAGACGAGGA GGAAAACACC 
TCCGAGTACA GTCACTCACC CTCCAGCCAG CACTCGACAC TAGAGAGCAA TACCGACATT 
GCGGACATGA AGAAAACTCA GGCCACATCC ACCTCAAGCA ACAGCTATAG CGAGCCCGAA 
GACGATTCGA GTGATTCCAG CGATGAGGAG CAGGGAAACC GGCCGACGGA AGCCAAGGCC 
GAGGGTCCAG CTTTGCCTAT GGGCGCCGTA CGCAGCAGCG ATATTATATC CATACCTACT 
TTTGAGGCTG ATGGCGCCGT CAATATGGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189132.1
CDS	663..3515
Translation	MVYIIPFFSHNDPPDMIITKEKSMACLQDEFGHLGIAATDLPFKSSDLDSPPRLQHHNNYAEITDSSAENTCQQRWPPHVGGAGAAFGHPDKPIGWMYGLLGCMKPVLSFIGKTGVIEVKSQRSEDWQIPFESITELEWLGSGAQGAVFSGRLKNETVAVKKVKELKETDIKHLRKLDHENIIKFKGVCTQSPVFCIIMEFCPYGPLQNILKEEQVMLPSRLVSWSKQIALGMQYLHSHKIIHRDLKSPNILISTNEVVKISDFGTSREWNEISTKMSFAGTVAWMAPEVIRNEPCSEKVDIWSYGVVLWEMLTCEIPYKDVDSSAIIWGVGNNSLKLLVPSTCPEGFKLLVKLCWKSKPRNRPSFRQILSHLDIAGPELLRKTEKQYFETQKSWKEEVRSHLKEITQNGTNIHKYEQDLIKRRTAEWRHAQDIRMVYEDKLQKTNQLFFELSECMSQLQEKEKEIAERERKLPGSGYKPNRRFGNTIRKMQHYRRRLNPAPAAIQQQSTTPDPETTPESPVKCMLYAQLDSNCQPKSYLANIIPSSGLGAPMPNKNKKVFRHRRNASGSFGAPPKYSPTRDRRYQSEPENRKVQLVERQTQTDAMDVSETDISPSAEAPRSQPIDVPVPNHRQLPLQLQRVQKIAQAQARARSGSTSSAAGAVNPACPSNGNSLSTSELTYQDACSSPDQLIDDVMNSNERLDMTECCSDNENLERLGRKVIEFINENRLSIQSNTNSVSNADNGNGGASPLELRESGNSPCLSRCSSTHSKRRKHPLGDNPNGSSIGNANGESHEQDSWSDEEGETTDYKYALRRRSIGRQPIARGMRPRRSYKAPLSQKIAIHKRNVVIVSDEEENTSEYSHSPSSQHSTLESNTDIADMKKTQATSTSSNSYSEPEDDSSDSSDEEQGNRPTEAKAEGPALPMGAVRSSDIISIPTFEADGAVNMV

Target ORF information:

RefSeq Version	NM_001202203.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wallenda (wnd), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202203.1

ORF Insert Sequence:

ATGGTCTACA TCATACCGTT CTTTAGCCAC AATGACCCGC CCGACATGAT CATCACCAAA 
GAAAAAAGCA TGGCTTGCCT GCAGGATGAG TTCGGTCACC TAGGCATCGC CGCTACAGAC 
CTGCCGTTCA AATCCTCCGA CCTGGACTCT CCGCCCCGCC TGCAGCATCA CAACAACTAT 
GCGGAGATCA CGGACAGCAG TGCGGAAAAC ACGTGCCAAC AGCGCTGGCC ACCTCATGTG 
GGAGGAGCAG GGGCGGCCTT CGGGCATCCG GACAAGCCCA TTGGCTGGAT GTACGGACTG 
CTCGGATGCA TGAAGCCCGT GCTTTCGTTC ATCGGGAAGA CGGGGGTGAT CGAGGTGAAG 
AGTCAGCGGA GCGAGGACTG GCAGATTCCA TTTGAGTCCA TCACCGAGTT GGAATGGCTG 
GGAAGCGGAG CACAAGGAGC CGTCTTCAGT GGCAGGCTAA AGAACGAGAC TGTGGCGGTC 
AAGAAGGTGA AGGAGCTGAA GGAAACGGAC ATCAAGCATC TCAGGAAGCT CGATCATGAG 
AACATTATTA AGTTCAAAGG GGTTTGCACG CAGTCACCGG TGTTCTGCAT CATCATGGAG 
TTCTGTCCGT ATGGCCCACT GCAGAATATC CTGAAGGAGG AGCAAGTCAT GTTACCGTCC 
CGCTTGGTCT CTTGGTCCAA GCAAATTGCC CTGGGCATGC AGTATCTGCA TTCCCACAAG 
ATCATACACC GAGATCTAAA GAGTCCAAAC ATACTGATTA GTACAAATGA GGTGGTGAAG 
ATCAGCGACT TTGGCACCAG TCGCGAGTGG AACGAGATCA GCACCAAGAT GAGCTTTGCT 
GGTACCGTAG CCTGGATGGC TCCGGAAGTG ATCAGGAATG AGCCGTGCTC CGAGAAGGTG 
GACATTTGGT CGTACGGTGT GGTGCTATGG GAAATGCTCA CCTGCGAGAT ACCTTACAAG 
GATGTGGACT CATCAGCCAT AATCTGGGGT GTGGGCAACA ACTCTCTCAA GCTGCTGGTC 
CCGAGCACCT GTCCGGAGGG ATTTAAGTTG CTGGTTAAGC TCTGTTGGAA AAGTAAGCCC 
CGAAATCGGC CATCGTTCCG GCAGATACTC TCGCACTTGG ATATCGCCGG ACCGGAACTG 
CTGAGAAAAA CCGAGAAGCA ATATTTTGAG ACGCAAAAGT CGTGGAAGGA GGAGGTGAGA 
TCTCACTTGA AGGAGATTAC CCAAAATGGC ACCAACATAC ACAAGTACGA GCAAGATCTG 
ATTAAGCGGC GGACGGCAGA GTGGCGACAT GCCCAGGATA TCCGAATGGT GTACGAGGAT 
AAGTTGCAAA AGACCAACCA GCTTTTCTTC GAACTCAGCG AGTGCATGTC CCAGTTGCAG 
GAAAAGGAGA AGGAAATCGC AGAACGAGAA CGGAAACTAC CAGGAAGTGG CTACAAACCC 
AATCGACGTT TTGGCAACAC GATCCGAAAA ATGCAACACT ATCGCCGACG CCTGAACCCT 
GCTCCTGCCG CCATTCAGCA ACAATCAACA ACGCCGGATC CAGAGACTAC GCCGGAGTCC 
CCTGTGAAGT GTATGCTATA TGCCCAGCTT GATAGCAATT GCCAACCCAA ATCGTATCTG 
GCCAATATCA TCCCGAGTAG CGGCTTAGGC GCACCCATGC CCAATAAAAA CAAGAAGGTC 
TTCCGGCACC GACGCAACGC ATCTGGTTCC TTCGGTGCAC CACCCAAGTA CAGTCCGACG 
AGGGATCGTC GTTACCAGAG CGAACCGGAG AACCGAAAGG TGCAGCTGGT GGAGAGACAA 
ACCCAAACAG ATGCCATGGA CGTGAGTGAA ACGGATATTA GTCCTAGCGC GGAGGCTCCT 
CGATCTCAGC CCATCGACGT GCCAGTTCCG AATCATCGCC AACTGCCGCT GCAACTGCAG 
AGAGTACAGA AAATTGCCCA AGCTCAGGCG AGGGCTCGAA GTGGAAGCAC TTCATCTGCA 
GCTGGCGCCG TGAATCCCGC TTGTCCGTCG AATGGCAACT CTCTGAGCAC AAGCGAGCTG 
ACCTACCAAG ATGCCTGCTC TAGTCCCGAT CAGCTCATCG ACGATGTAAT GAACAGCAAC 
GAACGTTTGG ACATGACTGA ATGCTGCAGT GACAACGAAA ACCTGGAACG TCTAGGCCGC 
AAAGTCATCG AATTCATCAA CGAAAACCGC CTGTCCATTC AGTCAAACAC GAACTCCGTG 
AGCAATGCCG ATAATGGGAA TGGAGGAGCC TCTCCTCTTG AATTGCGTGA GAGTGGTAAC 
AGTCCGTGTT TAAGTCGATG CAGCAGCACA CATAGCAAAC GGCGGAAGCA TCCACTGGGG 
GACAATCCAA ACGGCAGTTC GATAGGCAAT GCCAACGGGG AGTCGCATGA GCAGGACAGC 
TGGTCGGACG AGGAGGGCGA GACCACGGAC TACAAGTACG CTCTGCGGAG AAGAAGCATT 
GGCCGCCAGC CCATAGCTCG TGGCATGCGA CCCCGTCGCA GCTACAAGGC TCCACTCTCC 
CAGAAGATTG CCATCCACAA GCGCAATGTG GTAATCGTAT CAGACGAGGA GGAAAACACC 
TCCGAGTACA GTCACTCACC CTCCAGCCAG CACTCGACAC TAGAGAGCAA TACCGACATT 
GCGGACATGA AGAAAACTCA GGCCACATCC ACCTCAAGCA ACAGCTATAG CGAGCCCGAA 
GACGATTCGA GTGATTCCAG CGATGAGGAG CAGGGAAACC GGCCGACGGA AGCCAAGGCC 
GAGGGTCCAG CTTTGCCTAT GGGCGCCGTA CGCAGCAGCG ATATTATATC CATACCTACT 
TTTGAGGCTG ATGGCGCCGT CAATATGGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wnd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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