asf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	asf1
Entrez Gene ID	40141
Full Name	anti-silencing factor 1
Synonyms	ASF1,Asf1,CG9383,Dmel\CG9383,dASF/SF2,dASF1,dAsf1,l(3)76BDq
General protein information	Preferred Names anti-silencing factor 1 Names CG9383 gene product from transcript CG9383-RA CG9383-PA CG9383-PB anti- silencing function 1 anti-silencing factor asf1-PA asf1-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76B9-76B9\|3-46.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202202.2	NP_001189131.1	anti-silencing factor 1, isoform B
NM_079439.4	NP_524163.1	anti-silencing factor 1, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	asf1	NP_001189131.1
Saccharomyces cerevisiae (baker's yeast)	ASF1	NP_012420.1
Bos taurus (cattle)	ASF1A	NP_001069961.1
Mus musculus (house mouse)	Asf1a	NP_079817.1
Gallus gallus (chicken)	ASF1A	NP_001038155.1
Danio rerio (zebrafish)	asf1ba	NP_996946.1
Danio rerio (zebrafish)	LOC100535896	XP_003198211.1
Xenopus tropicalis (tropical clawed frog)	asf1b	NP_001004974.1
Homo sapiens (human)	ASF1A	NP_054753.1
Pan troglodytes (chimpanzee)	ASF1A	XP_003311502.1
Rattus norvegicus (Norway rat)	Asf1a	NP_001099859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005920	XP_315950.4
Caenorhabditis elegans (roundworm)	asfl-1	NP_492567.3
Caenorhabditis elegans (roundworm)	unc-85	NP_494837.2
Arabidopsis thaliana (thale cress)	SGA2	NP_176846.1
	ASF1A	XP_001110511.1
Danio rerio (zebrafish)	asf1bb	NP_999922.1
Arabidopsis thaliana (thale cress)	ASF1B	NP_198627.1
Canis lupus familiaris (dog)	ASF1A	XP_533479.1
Oryza sativa (rice)	Os05g0553800	NP_001056265.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DLP and ASF1 are part of a predeposition complex, which is recruited by XNP and is necessary to prevent DNA exposure in the nucleus.
Title: The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation.

ASF1 cellular levels are tightly controlled by distinct pathways and provide a molecular mechanism for post-translational regulation of dASF1 and hASF1a by TLK kinases.
Title: Phosphorylation-mediated control of histone chaperone ASF1 levels by Tousled-like kinases.

Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during NOTCH silencing.
Title: Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during NOTCH silencing.

Asf1 is directed via interactions with sequence-specific complexes to mediate silencing of specific target genes.
Title: Gene-specific targeting of the histone chaperone asf1 to mediate silencing.

dASF1 has a direct role in modifying chromatin structure during DNA replication and this function of dASF1 is important for the processivity of the replication machinery.
Title: The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication.

The following asf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18992	NM_079439.4 Latest version!	Drosophila melanogaster anti-silencing factor 1 (asf1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa18992	NM_001202202.2 Latest version!	Drosophila melanogaster anti-silencing factor 1 (asf1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asf1 ( NM_001202202.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189131.1 Drosophila melanogaster anti-silencing factor 1 (asf1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18992
Clone ID Related Accession (Same CDS sequence)	NM_001202202.2 , NM_079439.4
Accession Version	NM_001202202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anti-silencing factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202202.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGG TGCACATCAC CAACGTGGTG GTGCTGGACA ACCCGAGCAG CTTCTTCAAC 
CCCTTTCAGT TCGAACTCAC GTTCGAGTGC ATTGAGGAGC TAAAAGAGGA TCTAGAGTGG 
AAGATGATCT ATGTAGGCTC GGCGGAGTCC GAGGAGCACG ACCAGGTGCT GGACACCATA 
TACGTGGGAC CTGTGCCTGA GGGGCGACAC ATCTTTGTCT TCCAGGCGGA TCCGCCAGAC 
GTCAGCAAGA TCCCGGAACC GGATGCGGTG GGCGTTACCA TTGTTCTGCT TACCTGCTCC 
TACCGCGGCC AGGAGTTCGT CCGCGTGGGC TACTATGTGA ATAACGACTA CGCTGACCCG 
GAGATGCGCG AGAACCCGCC AACAAAGCCT CTTTTCGAGA AGCTGACGCG AAACATCCTG 
GCCAGCAAGC CGCGCGTCAC CCGATTCAAA ATCAATTGGG ACTACGGGCA CATCAACGGG 
AATGGAAATG GCGTGGAGAA CGGCCATCAA GATGAGATGG CCACCGATGG ACCGTCCACA 
TCGGAGGCGG CTTCAGCAGT TATCCATCCC GAAGATGATA ATAGTTTGGC CATGCCAATG 
GAGAACGGAA TCAAGGCCTT GAACGAGAAC TCCAACTCAC TGGCCATGGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189131.1
CDS	286..942
Translation	MAKVHITNVVVLDNPSSFFNPFQFELTFECIEELKEDLEWKMIYVGSAESEEHDQVLDTIYVGPVPEGRHIFVFQADPPDVSKIPEPDAVGVTIVLLTCSYRGQEFVRVGYYVNNDYADPEMRENPPTKPLFEKLTRNILASKPRVTRFKINWDYGHINGNGNGVENGHQDEMATDGPSTSEAASAVIHPEDDNSLAMPMENGIKALNENSNSLAMEC

Target ORF information:

RefSeq Version	NM_001202202.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anti-silencing factor 1 (asf1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202202.2

ORF Insert Sequence:

ATGGCCAAGG TGCACATCAC CAACGTGGTG GTGCTGGACA ACCCGAGCAG CTTCTTCAAC 
CCCTTTCAGT TCGAACTCAC GTTCGAGTGC ATTGAGGAGC TAAAAGAGGA TCTAGAGTGG 
AAGATGATCT ATGTAGGCTC GGCGGAGTCC GAGGAGCACG ACCAGGTGCT GGACACCATA 
TACGTGGGAC CTGTGCCTGA GGGGCGACAC ATCTTTGTCT TCCAGGCGGA TCCGCCAGAC 
GTCAGCAAGA TCCCGGAACC GGATGCGGTG GGCGTTACCA TTGTTCTGCT TACCTGCTCC 
TACCGCGGCC AGGAGTTCGT CCGCGTGGGC TACTATGTGA ATAACGACTA CGCTGACCCG 
GAGATGCGCG AGAACCCGCC AACAAAGCCT CTTTTCGAGA AGCTGACGCG AAACATCCTG 
GCCAGCAAGC CGCGCGTCAC CCGATTCAAA ATCAATTGGG ACTACGGGCA CATCAACGGG 
AATGGAAATG GCGTGGAGAA CGGCCATCAA GATGAGATGG CCACCGATGG ACCGTCCACA 
TCGGAGGCGG CTTCAGCAGT TATCCATCCC GAAGATGATA ATAGTTTGGC CATGCCAATG 
GAGAACGGAA TCAAGGCCTT GAACGAGAAC TCCAACTCAC TGGCCATGGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asf1 ( NM_079439.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524163.1 Drosophila melanogaster anti-silencing factor 1 (asf1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18992
Clone ID Related Accession (Same CDS sequence)	NM_001202202.2 , NM_079439.4
Accession Version	NM_079439.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anti-silencing factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079439.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGG TGCACATCAC CAACGTGGTG GTGCTGGACA ACCCGAGCAG CTTCTTCAAC 
CCCTTTCAGT TCGAACTCAC GTTCGAGTGC ATTGAGGAGC TAAAAGAGGA TCTAGAGTGG 
AAGATGATCT ATGTAGGCTC GGCGGAGTCC GAGGAGCACG ACCAGGTGCT GGACACCATA 
TACGTGGGAC CTGTGCCTGA GGGGCGACAC ATCTTTGTCT TCCAGGCGGA TCCGCCAGAC 
GTCAGCAAGA TCCCGGAACC GGATGCGGTG GGCGTTACCA TTGTTCTGCT TACCTGCTCC 
TACCGCGGCC AGGAGTTCGT CCGCGTGGGC TACTATGTGA ATAACGACTA CGCTGACCCG 
GAGATGCGCG AGAACCCGCC AACAAAGCCT CTTTTCGAGA AGCTGACGCG AAACATCCTG 
GCCAGCAAGC CGCGCGTCAC CCGATTCAAA ATCAATTGGG ACTACGGGCA CATCAACGGG 
AATGGAAATG GCGTGGAGAA CGGCCATCAA GATGAGATGG CCACCGATGG ACCGTCCACA 
TCGGAGGCGG CTTCAGCAGT TATCCATCCC GAAGATGATA ATAGTTTGGC CATGCCAATG 
GAGAACGGAA TCAAGGCCTT GAACGAGAAC TCCAACTCAC TGGCCATGGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524163.1
CDS	286..942
Translation	MAKVHITNVVVLDNPSSFFNPFQFELTFECIEELKEDLEWKMIYVGSAESEEHDQVLDTIYVGPVPEGRHIFVFQADPPDVSKIPEPDAVGVTIVLLTCSYRGQEFVRVGYYVNNDYADPEMRENPPTKPLFEKLTRNILASKPRVTRFKINWDYGHINGNGNGVENGHQDEMATDGPSTSEAASAVIHPEDDNSLAMPMENGIKALNENSNSLAMEC

Target ORF information:

RefSeq Version	NM_079439.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anti-silencing factor 1 (asf1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079439.4

ORF Insert Sequence:

ATGGCCAAGG TGCACATCAC CAACGTGGTG GTGCTGGACA ACCCGAGCAG CTTCTTCAAC 
CCCTTTCAGT TCGAACTCAC GTTCGAGTGC ATTGAGGAGC TAAAAGAGGA TCTAGAGTGG 
AAGATGATCT ATGTAGGCTC GGCGGAGTCC GAGGAGCACG ACCAGGTGCT GGACACCATA 
TACGTGGGAC CTGTGCCTGA GGGGCGACAC ATCTTTGTCT TCCAGGCGGA TCCGCCAGAC 
GTCAGCAAGA TCCCGGAACC GGATGCGGTG GGCGTTACCA TTGTTCTGCT TACCTGCTCC 
TACCGCGGCC AGGAGTTCGT CCGCGTGGGC TACTATGTGA ATAACGACTA CGCTGACCCG 
GAGATGCGCG AGAACCCGCC AACAAAGCCT CTTTTCGAGA AGCTGACGCG AAACATCCTG 
GCCAGCAAGC CGCGCGTCAC CCGATTCAAA ATCAATTGGG ACTACGGGCA CATCAACGGG 
AATGGAAATG GCGTGGAGAA CGGCCATCAA GATGAGATGG CCACCGATGG ACCGTCCACA 
TCGGAGGCGG CTTCAGCAGT TATCCATCCC GAAGATGATA ATAGTTTGGC CATGCCAATG 
GAGAACGGAA TCAAGGCCTT GAACGAGAAC TCCAACTCAC TGGCCATGGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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asf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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