ASF1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASF1A
Entrez Gene ID	25842
Full Name	anti-silencing function 1A histone chaperone
Synonyms	CGI-98,CIA,HSPC146
General protein information	Preferred Names anti-silencing function 1A histone chaperone Names histone chaperone ASF1A ASF1 anti-silencing function 1 homolog A CCG1-interacting factor A anti-silencing function protein 1 homolog A hAsf1 hAsf1a hCIA
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q22.31
Summary	This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008].
Disorder MIM:	609189

mRNA and Protein(s)

mRNA	Protein	Name
NM_014034.2	NP_054753.1	histone chaperone ASF1A
NM_014034.3	NP_054753.1	histone chaperone ASF1A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	asf1	NP_001189131.1
Saccharomyces cerevisiae (baker's yeast)	ASF1	NP_012420.1
Bos taurus (cattle)	ASF1A	NP_001069961.1
Mus musculus (house mouse)	Asf1a	NP_079817.1
Gallus gallus (chicken)	ASF1A	NP_001038155.1
Danio rerio (zebrafish)	asf1ba	NP_996946.1
Danio rerio (zebrafish)	LOC100535896	XP_003198211.1
Xenopus tropicalis (tropical clawed frog)	asf1b	NP_001004974.1
Homo sapiens (human)	ASF1A	NP_054753.1
Pan troglodytes (chimpanzee)	ASF1A	XP_003311502.1
Rattus norvegicus (Norway rat)	Asf1a	NP_001099859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005920	XP_315950.4
Caenorhabditis elegans (roundworm)	asfl-1	NP_492567.3
Caenorhabditis elegans (roundworm)	unc-85	NP_494837.2
Arabidopsis thaliana (thale cress)	SGA2	NP_176846.1
	ASF1A	XP_001110511.1
Danio rerio (zebrafish)	asf1bb	NP_999922.1
Arabidopsis thaliana (thale cress)	ASF1B	NP_198627.1
Canis lupus familiaris (dog)	ASF1A	XP_533479.1
Oryza sativa (rice)	Os05g0553800	NP_001056265.1

Gene Ontology

The following ASF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16371	NM_014034.3 Latest version!	Homo sapiens anti-silencing function 1A histone chaperone (ASF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16371	NM_014034.2	Homo sapiens anti-silencing function 1A histone chaperone (ASF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASF1A ( NM_014034.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_054753.1 Homo sapiens anti-silencing function 1A histone chaperone (ASF1A), mRNA.

Price & Availability↑

CloneID	OHu16371
Clone ID Related Accession (Same CDS sequence)	NM_014034.2 , NM_014034.3
Accession Version	NM_014034.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	histone chaperone ASF1A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA902777.1, AK025738.1 and AA481056.1. On Sep 30, 2006 this sequence version replaced NM_014034.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.145014.1, SRR1803613.286373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAAGG TTCAGGTGAA CAATGTAGTG GTGCTGGATA ACCCTTCTCC TTTCTACAAC 
CCGTTCCAGT TCGAGATCAC CTTCGAGTGC ATCGAGGACC TGTCTGAAGA CTTGGAATGG 
AAAATTATCT ATGTGGGCTC TGCAGAAAGT GAAGAATACG ATCAAGTTTT AGACTCTGTT 
TTAGTGGGTC CTGTTCCCGC AGGAAGGCAT ATGTTTGTAT TTCAGGCTGA TGCACCTAAT 
CCAGGACTCA TTCCAGATGC AGATGCAGTA GGCGTAACTG TTGTGCTAAT TACTTGTACC 
TATCGAGGAC AAGAATTTAT TAGAGTTGGC TATTATGTAA ATAATGAATA TACTGAGACA 
GAATTAAGGG AAAATCCACC AGTAAAACCA GACTTTTCTA AGCTTCAAAG GAATATTTTG 
GCATCTAATC CCAGGGTCAC AAGATTCCAC ATTAATTGGG AAGATAACAC AGAAAAACTG 
GAAGATGCAG AGAGCAGTAA TCCAAATCTA CAGTCACTTC TTTCAACAGA TGCATTACCT 
TCAGCATCAA AGGGATGGTC CACATCAGAA AACTCACTAA ATGTCATGTT AGAATCCCAC 
ATGGACTGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054753.1
CDS	338..952
Translation	MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

Target ORF information:

RefSeq Version	NM_014034.2
Organism	Homo sapiens(human)
Definition	Homo sapiens anti-silencing function 1A histone chaperone (ASF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014034.2

ORF Insert Sequence:

ATGGCAAAGG TTCAGGTGAA CAATGTAGTG GTGCTGGATA ACCCTTCTCC TTTCTACAAC 
CCGTTCCAGT TCGAGATCAC CTTCGAGTGC ATCGAGGACC TGTCTGAAGA CTTGGAATGG 
AAAATTATCT ATGTGGGCTC TGCAGAAAGT GAAGAATACG ATCAAGTTTT AGACTCTGTT 
TTAGTGGGTC CTGTTCCCGC AGGAAGGCAT ATGTTTGTAT TTCAGGCTGA TGCACCTAAT 
CCAGGACTCA TTCCAGATGC AGATGCAGTA GGCGTAACTG TTGTGCTAAT TACTTGTACC 
TATCGAGGAC AAGAATTTAT TAGAGTTGGC TATTATGTAA ATAATGAATA TACTGAGACA 
GAATTAAGGG AAAATCCACC AGTAAAACCA GACTTTTCTA AGCTTCAAAG GAATATTTTG 
GCATCTAATC CCAGGGTCAC AAGATTCCAC ATTAATTGGG AAGATAACAC AGAAAAACTG 
GAAGATGCAG AGAGCAGTAA TCCAAATCTA CAGTCACTTC TTTCAACAGA TGCATTACCT 
TCAGCATCAA AGGGATGGTC CACATCAGAA AACTCACTAA ATGTCATGTT AGAATCCCAC 
ATGGACTGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASF1A ( NM_014034.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054753.1 Homo sapiens anti-silencing function 1A histone chaperone (ASF1A), mRNA.

Price & Availability↑

CloneID	OHu16371
Clone ID Related Accession (Same CDS sequence)	NM_014034.2 , NM_014034.3
Accession Version	NM_014034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	histone chaperone ASF1A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA902777.1, AK025738.1 and AA481056.1. On Nov 23, 2018 this sequence version replaced NM_014034.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.80138.1, SRR1660805.154207.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229595.6/ ENSP00000229595.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAAAGG TTCAGGTGAA CAATGTAGTG GTGCTGGATA ACCCTTCTCC TTTCTACAAC 
CCGTTCCAGT TCGAGATCAC CTTCGAGTGC ATCGAGGACC TGTCTGAAGA CTTGGAATGG 
AAAATTATCT ATGTGGGCTC TGCAGAAAGT GAAGAATACG ATCAAGTTTT AGACTCTGTT 
TTAGTGGGTC CTGTTCCCGC AGGAAGGCAT ATGTTTGTAT TTCAGGCTGA TGCACCTAAT 
CCAGGACTCA TTCCAGATGC AGATGCAGTA GGCGTAACTG TTGTGCTAAT TACTTGTACC 
TATCGAGGAC AAGAATTTAT TAGAGTTGGC TATTATGTAA ATAATGAATA TACTGAGACA 
GAATTAAGGG AAAATCCACC AGTAAAACCA GACTTTTCTA AGCTTCAAAG GAATATTTTG 
GCATCTAATC CCAGGGTCAC AAGATTCCAC ATTAATTGGG AAGATAACAC AGAAAAACTG 
GAAGATGCAG AGAGCAGTAA TCCAAATCTA CAGTCACTTC TTTCAACAGA TGCATTACCT 
TCAGCATCAA AGGGATGGTC CACATCAGAA AACTCACTAA ATGTCATGTT AGAATCCCAC 
ATGGACTGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054753.1
CDS	263..877
Translation	MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

Target ORF information:

RefSeq Version	NM_014034.3
Organism	Homo sapiens(human)
Definition	Homo sapiens anti-silencing function 1A histone chaperone (ASF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014034.3

ORF Insert Sequence:

ATGGCAAAGG TTCAGGTGAA CAATGTAGTG GTGCTGGATA ACCCTTCTCC TTTCTACAAC 
CCGTTCCAGT TCGAGATCAC CTTCGAGTGC ATCGAGGACC TGTCTGAAGA CTTGGAATGG 
AAAATTATCT ATGTGGGCTC TGCAGAAAGT GAAGAATACG ATCAAGTTTT AGACTCTGTT 
TTAGTGGGTC CTGTTCCCGC AGGAAGGCAT ATGTTTGTAT TTCAGGCTGA TGCACCTAAT 
CCAGGACTCA TTCCAGATGC AGATGCAGTA GGCGTAACTG TTGTGCTAAT TACTTGTACC 
TATCGAGGAC AAGAATTTAT TAGAGTTGGC TATTATGTAA ATAATGAATA TACTGAGACA 
GAATTAAGGG AAAATCCACC AGTAAAACCA GACTTTTCTA AGCTTCAAAG GAATATTTTG 
GCATCTAATC CCAGGGTCAC AAGATTCCAC ATTAATTGGG AAGATAACAC AGAAAAACTG 
GAAGATGCAG AGAGCAGTAA TCCAAATCTA CAGTCACTTC TTTCAACAGA TGCATTACCT 
TCAGCATCAA AGGGATGGTC CACATCAGAA AACTCACTAA ATGTCATGTT AGAATCCCAC 
ATGGACTGCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.