ash1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ash1
Entrez Gene ID	40133
Full Name	absent, small, or homeotic discs 1
Synonyms	ASH-1,ASH1,Ash,Ash 1,Ash1,CG8887,Dmel\CG8887,ash,ash-1,dash,l(3)76BDb,l(3)SG29a
General protein information	Preferred Names absent, small, or homeotic discs 1 Names CG8887 gene product from transcript CG8887-RB Absent, small or homeotic discs 1 Absent, small, or homeotic disc 1 CG8887-PB CG8887-PC absent small or homeotic 1 ash1-PB ash1-PC discs absent, small, or homeotic discs absent, small, or homeotic-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76B8-76B9\|3-46.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079436.3	NP_524160.2	absent, small, or homeotic discs 1, isoform B
NM_001259905.1	NP_001246834.1	absent, small, or homeotic discs 1, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

If ASH1 or TRX function is lost or reduced, heterochromatin can spread into these domains creating a sink that diverts heterochromatic proteins from other variegating locations, which then may express a suppressed phenotype.
Title: Mutations in ash1 and trx enhance P-element-dependent silencing in Drosophila melanogaster.

loss of ASH1 significantly decreases H3K36 dimethylation, providing further evidence that ASH1 is an H3K36 dimethylase in vivo.
Title: The trithorax group proteins Kismet and ASH1 promote H3K36 dimethylation to counteract Polycomb group repression in Drosophila.

Physical interaction of ASH1 with FSH activates global transcription.
Title: The BET protein FSH functionally interacts with ASH1 to orchestrate global gene activity in Drosophila.

lysine to arginine substitution of histone H3 at position 36 abolished the methyltransferase activity of Drosophila ASH1, suggesting that K36 is their specific target
Title: Trithorax-group protein ASH1 methylates histone H3 lysine 36.

implication that ASH1 is required for essentially all H3-K4 methylation that occurs in vivo
Title: ASH1, a Drosophila trithorax group protein, is required for methylation of lysine 4 residues on histone H3.

The following ash1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ash1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18982	NM_079436.3 Latest version!	Drosophila melanogaster absent, small, or homeotic discs 1 (ash1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa18982	NM_001259905.1 Latest version!	Drosophila melanogaster absent, small, or homeotic discs 1 (ash1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ash1 ( NM_079436.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524160.2 Drosophila melanogaster absent, small, or homeotic discs 1 (ash1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18982
Clone ID Related Accession (Same CDS sequence)	NM_079436.3 , NM_001259905.1
Accession Version	NM_079436.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	absent, small, or homeotic discs 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079436.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGTA GCCAAAATGA GACGGCAGCA GCAAAGGTTC TGGAAACGCA ACGCGCACAA 
GAATCCGGCA GTGAAAATGA GGAAACCGAT TCCATTACGG ATCAATCGAG CCAGTCGAAG 
TCGATCAAGT CAGCCACCCA GTTTAGCGTG CAGCGTTCGG ACACCGATGG ACTGCGAATG 
AGAATCTCGG CCATCCGCCC CACGTTAGGA GTCGTAGCAA CCAAGAAACC CCCAAAGTCC 
AGAAAAATGT CTACCCAGGA CACCGAGTCC GGCTGCTCGG AGGCCAAAAA TAGAGCGGTC 
AGCAAGAAAG TGAAGGTCAA GCGCAAGAAG CTGGCAAGTT CCAGTGGGAT TAGTAAATCG 
GACAAAGTGT CTAAGTCTAA GAAGTCACAG ATCTCGGCAT TTTCCTCGGA CTCAGAGGAC 
GATCTGCCCT TGAAGGTGCA TCAGCAGAGA GCTCCGCGAG TGCTGCTAAG TGCTATCATC 
CAGGCGGCAC AGTCGGCCAG CAAACCCACC CTCGATATCG GAATCTCGTC CAGCGACAAC 
GAGTTACCTA ACCTGGTGCA GGCGGCTATC AAGCGAGTGG AGAGCGATAC GGAGGACACA 
ACTGTGGAAG GAAGTTTCCG CAAAGCGGCC AAGGACAAGA ACCTACCCCA GTACCAGTCA 
ACTTTGCTGC AAGACTTCAT GGAGAAGACT CAGATGCTGG GGCAGACCGT CAATGCGAAG 
CTTGCGGAGG AGAAAGTGGC TAAAGCTAAG GAGGAGACCC TAGTCCAAAC AGCTGTTCCT 
CGAAAGCGCA GAGGTAGACC CAAAAAAGTG GTTCCCACAG TTCCTGCCCC AGGAAACTCT 
GGCCCTGCTA TAAACGAATC TGCCGATTCG GGTGTGATAA GCACCACTAG CACAACGCAG 
AGCACTACTC CCTCCCCGAA AATGCAAAAT GAGAATGCTG TGCCGACGGG ATCGCTGCCA 
ATCGCCTCCA GCAGCAAGCC TAAGATCGAC ATGGCGTATC TGGACAAGCG AATGTATGCC 
ACTGAGCGGG TGCTCTATCC ACCGCCCAGG AGTAAGCGAC GGCAGAACAA TAAAAAGACA 
GCCTGCAGTT CATCCAACAA AGAGGAACTT CAGCTTGATC CGCTGTGGCG AGAGATCGAC 
GTGAACAAGA AGTTTAGGCT GAGGAGTATG AGTGTGGGTG CGGCTAGTGG AACAGGAGCA 
AGCACCACTA TTTGCAGTAA GGTCTTGGCC GCTAAGAGCG GTTACGTCTC GGATTACGGA 
AGCGTACGAC ATCAGCGGAG CAGCCACAAC CACAACTCCG GTTACAAGTC CGATGCCAGC 
TGCAAGAGTC GATACAGCAC CAAGAGTTGT ATGAGTCGCA GGAGCAGGGC AAAGAGCTGC 
GGCTACCGGA GTGATTGCAA GGAATCTGGA AAGTCAGGCC TAAGGATGAG GCGGAAGCGC 
CGGGCTTCCA TGCTCTTGAA GAGCTCAGCA GACGATACTG TCGAGGACCA GGACATCCTT 
CAGCTAGCTG GATTATCTCT GGGCCAGAGC AGTGAGGAGA GCAACGAATA CATCAGTAAG 
CCGAGCCTTA AAAGCCTTCC CACGACAAGT GCCAGCAAGA AGTACGGCGA AATCAATCGC 
TATGTGACCA CCGGGCAGTA TTTCGGTCGA GGCGGTAGTT TGTCTGCCAC CAACCCGGAT 
AACTTCATTA GTAAAATGAT GAACCAGCGT AAAGAAACCC CGGCTCCCAG CAAATCGTCC 
TGCAAGATTA AATCTCGCCG CTCATCGGCA GCCAGTATGT GCAGCAGCTA TGTGTCTGGG 
GTGTCTAGAA TGCGTCGCAG ACATCGCCGG AAGAGTTTCA GTCACAACAA ATCATTGAAC 
ATTGATTCCA AGCTGCTCAC CGAAATCGAG ATAATCACAA GCACCTTCAA CTCAAGGTGT 
CGCATCCAAG ATGATCGTTT GACCGGAAGC AGTGGCAAAG AGAAGCTATT GGCCGATGCC 
AACAAGCTGC AGGCCACCCT AGCAGCACCA AGCCCTGCCC AGCAGTTGAC TCTGAATGGA 
GGAGGACCAG CTTCCACCCT TTCCAAACCA TTAAAACGGG GCCTAAAGAA GAGAAAGCTG 
AGTGAGCCCC TAGTGGACTT CGCTATGCTA TCAGCTAGCG CTAGTGGAAC TCCCAATGGC 
AGCGGAAGCA GTAATGGAAA TACCAAACGA CGACACAAGA AATCTCAAAG CAATGACAGT 
TCCAGTCCCG ATGATCACAA GTTGCCGTTG AAAAAGCGCC ACTATCTTTT GACTCCAGGA 
GAGCGTCCGC CGGCGGAAGT AGCATTCGCC AATGGGAAAT TGAACGCAGA GGCCTGGGCT 
GCAGCCGCGG CGGCTGCTAA GAGCACTGCG TCTACCAAAT CGCAAGCCCA GTTTAATGCT 
AGGAGCGTAA AATCTGCGCT AACGCCCAAA AAAAGACATC TCCTAGAGCA ACCTACGTCT 
GTGAGCGGAG CTGGCTCGTC GGCCAGTAAT TCTCCCCTGA GAATTGTTGT CGATAATAAT 
TCCATAAGTG GTGGAAAGTT GCTGGATATA AGTCCTAGTT CCTTGTGTTC CCTCAAACAG 
CAGAGGAGAG GAGGAGCAGC TAAACAGAAG GTGTCGGCAG CGAAGGACCT CGTTCAGCTT 
CAATCCCCAG CTGGTAGCTA TCCTCCCCCC GGTGTGTTCG AGCCATCTGT GGAGCTGGAA 
ATCCAAATTC CTCTTAGTAA ACTGAACGAA TCCGTCATAA CCAAAGCAGA GGTCGAATCT 
CCACTGCTCT CAGCATTGGA CATTAAGGAG GATACGAAAA AGGAGGTTGG CCAGCGCGTT 
GTCGAAACCT TGCTACACAA AACGGGAGGC AATCTTCTTC TGAAACGCAA GCGGAAAAAG 
ATAAATCGAA CTGGATTTCC CACTGTTCGC AGGAAGAAAC GTAAAGTTAG CGTGGAGCAG 
CAAACAACAG CCGTGATTGA TGAGCACGAG CCGGAGTTTG ATCCCGATGA TGAGCCACTG 
CAATCCCTAA GAGAGACCAG GAGTAGCAAT AATGTCAATG TGCAGGCGGC ACCTAATCCT 
CCACTGGATT GCGAGCGAGT TCCCCAAGCA GGCGAGGCCA GAGAAACCTT TGTGGCCAGG 
ACCAATCAAA AAGCCCCTCG ATTATCGGTG GTGGCCCTGG AGCGCCTACA GCGTCCTCAA 
ACACCAGCTA GAGGAAGACC GCGAGGTAGA AAACCTAAGA ACAGGGAACA AGCTGAAGCT 
GCACCTCAAC CGCCGCCCAA ATCGGAACCT GAGATAAGGC CAGCCAAAAA ACGTGGCCGG 
CAACCCAAGC AGCCGGTACT GGAAGAGCCA CCACCCACAC CACCTCCTCA ACAGAAAAAA 
AACAAAATGG AACCAAATAT TAGACTACCA GATGGCATCG ATCCCAATAC GAATTTCAGC 
TGCAAGATTC GCTTGAAGCG GCGAAAGAAC TTAGAGGCTG GAACCCAACC AAAAAAGGAG 
AAGCCAGTCC AGCCAGTGAC GGTGGAAGAG ATTCCACCAG AAATTCCCGT CAGTCAAGAA 
GAAATAGATG CAGAAGCAGA GGCTAAACGG CTAGACAGTA TTCCTACCGA GCACGATCCC 
TTGCCTGCCA GTGAGTCCCA CAACCCCGGT CCGCAGGACT ATGCCAGTTG CAGTGAATCC 
AGCGAGGACA AGGCATCAAC CACATCCTTG CGGAAACTAT CTAAGGTCAA GAAGACCTAT 
CTTGTAGCAG GACTCTTCTC GAATCATTAC AAACAATCCC TGATGCCGCC CCCAGCAAAG 
GTGAACAAAA AACCAGGCCT GGAGGAGCAA GTGGGTCCTG CGAGCCTTCT ACCACCACCT 
CCCTATTGCG AAAAGTACCT CCGAAGGACT GAAATGGACT TTGAGCTACC TTACGACATT 
TGGTGGGCTT ACACTAATTC CAAATTACCC ACTCGAAACG TTGTGCCATC CTGGAATTAC 
CGAAAGATCC GCACCAATGT ATACGCAGAG TCAGTGCGTC CAAATTTGGC GGGATTCGAT 
CATCCCACCT GTAACTGTAA GAACCAGGGC GAGAAGTCTT GTTTGGACAA CTGTCTCAAC 
CGCATGGTTT ATACGGAGTG CTCGCCCAGC AATTGCCCGG CTGGAGAGAA GTGCCGAAAT 
CAGAAGATCC AGCGACATGC CGTCGCACCC GGAGTGGAGC GCTTTATGAC GGCAGATAAA 
GGATGGGGAG TGCGAACTAA GCTACCCATT GCGAAGGGAA CCTACATTCT GGAGTACGTG 
GGCGAGGTAG TCACGGAAAA GGAATTCAAG CAGAGGATGG CCAGCATCTA CCTAAATGAC 
ACCCACCACT ATTGCTTACA TTTGGATGGA GGACTGGTTA TCGACGGTCA GCGGATGGGC 
AGCGATTGTA GGTTTGTCAA CCATTCTTGC GAACCCAATT GCGAGATGCA AAAATGGAGT 
GTTAACGGCC TATCTAGAAT GGTTTTATTC GCCAAAAGAG CCATAGAGGA GGGGGAGGAG 
CTGACATACG ATTACAACTT TTCGCTGTTC AATCCCTCAG AGGGTCAGCC CTGCAGGTGC 
AACACGCCCC AGTGTCGTGG TGTCATTGGT GGCAAGTCGC AGAGAGTTAA ACCCCTTCCT 
GCTGTGGAGG CCAAGCCATC TGGAGAGGGA CTTTCTGGCC GAAACGGGCG CCAACGGAAG 
CAGAAGGCCA AAAAGCATGC TCAACGGCAG GCGGGAAAAG ATATTTCATC AGCAGTGGCG 
GTGGCAAAGC TTCAACCATT GTCCGAAAAA GAAAAGAAAC TGGTCAGACA ATTCAACACA 
TTTCTAGTCA GGAACTTCGA AAAGATACGC AGATGCAAGG CCAAGCGGGC GTCAGATGCA 
GCGGCGACTG CATCCTCGCC AGCACTTGGC ACCACTAATG GGGACATTCC TGGTAGACGT 
CCCTCCACAC CATCTTCTCC TTCCTTGGCA GCGCAGATTT CCGCGCTCTG CTCGCCTCGC 
AACATAAAAA CCCGTGGACT CACACAGGCA GTGCATGATC CCGAACTAGA GAAAATGGCC 
AAAATGGCTG TAGTTCTAAG GGATATTTGC AGTGCCATGG AGACCCTCAA AATGTCCGAT 
TTGTTGACGA CAGTGTCCAG CAAGAAAAAG AAGCCTATAA AGACCACTTT GAGTGGAAAA 
TTGGGTTCTA CAGCTGCAAC TTCAAAAGTG GAATTCAGAT CGATACAAGC CCAGGTGGAG 
CAGGGACATT ACAAAACGCC GCAGGAATTC GATGACCACA TGCAGCAGCT CTTTGTGGAG 
GCCAAGCAGC AACACGGCGA TGATGAGGGC AAGGAAAAAG CGCTGCAGTC CCTGAAGGAT 
AGCTATGAGC AACAGAAGAT CGCCAGCTAT GTTCAGCTGG TGGAGATTCT TGGTGATTCG 
GAATCGTTGC AGAGCTTTAA ACCAAAGGAA GTGCTTTCGT CAGAGGAGGA ACCTGGAAAG 
ATAGCAGTGA AGAAATCACC AGGAGCAAAG GAAAGAGATT CCCCAATTGT GCCATTGAAA 
GTGACTCCGC CACCCCTGCT TCCTATTGAG GCCTCTCCTG ATGAGGATGT AATCCGATGT 
ATTTGTGGAT TGTACAAAGA TGAAGGCTTG ATGATTCAGT GCTCCAAATG CATGGTGTGG 
CAGCATACTG AATGTACCAA AGCTGACATC GATGCGGATA ATTATCAGTG CGAGCGTTGC 
GAGCCAAGGG AAGTGGATCG AGAAATTCCC CTGGAGGAAT TTACCGAAGA GGGACACCGT 
TACTATCTCT CCCTAATGCG TGGTGATCTG CAGGTACGAC AGGGCGATGC CGTCTATGTC 
CTACGAGACA TCCCCATCAA GGATGAGTCC GGCAAGGTGT TACCGACGAA GAAGCACACC 
TATGAAACGA TCGGAGCCAT TGATTACCAA GAGTGCGATA TCTTTCGGGT GGAGCACTTG 
TGGAAAAACG AACTGGGAAA GCGTTTTATA TTCGGACACC ATTTCCTGCG TCCTCATGAA 
ACTTTCCACG AACCATCGCG TCGTTTCTAC CCCAATGAGG TGGTACGAGT TTCACTTTAT 
GAGGTGGTAC CCATTGAGTT GGTCATTGGA CGCTGCTGGG TGTTGGATCG AACAACTTTC 
TGTAAAGGAC GCCCCATGGA ATGCAACGAT GAGGATCATT GCTACATCTG CGAGCTGCGT 
GTGGACAAGA CGGCAAGGTT CTTCTCAAAG GCCAAGGCCA ACCATCCAGC CTGCACCAAG 
AGCTATGCCT TCAGAAAATT TCCCGAGAAG ATTAAGATCT CCAAGAGCTA TGCGCCCCAT 
GATGTGGATC CGTCGTTGCT GAAGACAAGG AAGCAAAAGA CTGAATTAGA CGTAGGAGCC 
GGGCCAACAA CGATGCACAA GGTGTCTGGC AGGCAGGAAC AGCATCAGGC CAAGATGGTT 
GGGCGAAAGC CTCGTGGGAT CAGTGCCCCA GCGGATGCAA CTGCTGTCCA TGTCGTAACA 
CCCGTGGCGC CCAATAAACA GATGCTTAAG AAGAGGAAAT CGCGTTTAGA GAACGTTTTG 
ATAACTATGA AGCTTAAGTG TCTGGATGCA CAAACGGCAC AGGAGCAACC CATTGACTTG 
TCATACCTGC TGTCCGGACG CGGCGCCCGG CAGCGGAAGA CCCAGCAGTC CAGTAGCAGT 
TCTACGGCCA ACTCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524160.2
CDS	391..7071
Translation	MSCSQNETAAAKVLETQRAQESGSENEETDSITDQSSQSKSIKSATQFSVQRSDTDGLRMRISAIRPTLGVVATKKPPKSRKMSTQDTESGCSEAKNRAVSKKVKVKRKKLASSSGISKSDKVSKSKKSQISAFSSDSEDDLPLKVHQQRAPRVLLSAIIQAAQSASKPTLDIGISSSDNELPNLVQAAIKRVESDTEDTTVEGSFRKAAKDKNLPQYQSTLLQDFMEKTQMLGQTVNAKLAEEKVAKAKEETLVQTAVPRKRRGRPKKVVPTVPAPGNSGPAINESADSGVISTTSTTQSTTPSPKMQNENAVPTGSLPIASSSKPKIDMAYLDKRMYATERVLYPPPRSKRRQNNKKTACSSSNKEELQLDPLWREIDVNKKFRLRSMSVGAASGTGASTTICSKVLAAKSGYVSDYGSVRHQRSSHNHNSGYKSDASCKSRYSTKSCMSRRSRAKSCGYRSDCKESGKSGLRMRRKRRASMLLKSSADDTVEDQDILQLAGLSLGQSSEESNEYISKPSLKSLPTTSASKKYGEINRYVTTGQYFGRGGSLSATNPDNFISKMMNQRKETPAPSKSSCKIKSRRSSAASMCSSYVSGVSRMRRRHRRKSFSHNKSLNIDSKLLTEIEIITSTFNSRCRIQDDRLTGSSGKEKLLADANKLQATLAAPSPAQQLTLNGGGPASTLSKPLKRGLKKRKLSEPLVDFAMLSASASGTPNGSGSSNGNTKRRHKKSQSNDSSSPDDHKLPLKKRHYLLTPGERPPAEVAFANGKLNAEAWAAAAAAAKSTASTKSQAQFNARSVKSALTPKKRHLLEQPTSVSGAGSSASNSPLRIVVDNNSISGGKLLDISPSSLCSLKQQRRGGAAKQKVSAAKDLVQLQSPAGSYPPPGVFEPSVELEIQIPLSKLNESVITKAEVESPLLSALDIKEDTKKEVGQRVVETLLHKTGGNLLLKRKRKKINRTGFPTVRRKKRKVSVEQQTTAVIDEHEPEFDPDDEPLQSLRETRSSNNVNVQAAPNPPLDCERVPQAGEARETFVARTNQKAPRLSVVALERLQRPQTPARGRPRGRKPKNREQAEAAPQPPPKSEPEIRPAKKRGRQPKQPVLEEPPPTPPPQQKKNKMEPNIRLPDGIDPNTNFSCKIRLKRRKNLEAGTQPKKEKPVQPVTVEEIPPEIPVSQEEIDAEAEAKRLDSIPTEHDPLPASESHNPGPQDYASCSESSEDKASTTSLRKLSKVKKTYLVAGLFSNHYKQSLMPPPAKVNKKPGLEEQVGPASLLPPPPYCEKYLRRTEMDFELPYDIWWAYTNSKLPTRNVVPSWNYRKIRTNVYAESVRPNLAGFDHPTCNCKNQGEKSCLDNCLNRMVYTECSPSNCPAGEKCRNQKIQRHAVAPGVERFMTADKGWGVRTKLPIAKGTYILEYVGEVVTEKEFKQRMASIYLNDTHHYCLHLDGGLVIDGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGEELTYDYNFSLFNPSEGQPCRCNTPQCRGVIGGKSQRVKPLPAVEAKPSGEGLSGRNGRQRKQKAKKHAQRQAGKDISSAVAVAKLQPLSEKEKKLVRQFNTFLVRNFEKIRRCKAKRASDAAATASSPALGTTNGDIPGRRPSTPSSPSLAAQISALCSPRNIKTRGLTQAVHDPELEKMAKMAVVLRDICSAMETLKMSDLLTTVSSKKKKPIKTTLSGKLGSTAATSKVEFRSIQAQVEQGHYKTPQEFDDHMQQLFVEAKQQHGDDEGKEKALQSLKDSYEQQKIASYVQLVEILGDSESLQSFKPKEVLSSEEEPGKIAVKKSPGAKERDSPIVPLKVTPPPLLPIEASPDEDVIRCICGLYKDEGLMIQCSKCMVWQHTECTKADIDADNYQCERCEPREVDREIPLEEFTEEGHRYYLSLMRGDLQVRQGDAVYVLRDIPIKDESGKVLPTKKHTYETIGAIDYQECDIFRVEHLWKNELGKRFIFGHHFLRPHETFHEPSRRFYPNEVVRVSLYEVVPIELVIGRCWVLDRTTFCKGRPMECNDEDHCYICELRVDKTARFFSKAKANHPACTKSYAFRKFPEKIKISKSYAPHDVDPSLLKTRKQKTELDVGAGPTTMHKVSGRQEQHQAKMVGRKPRGISAPADATAVHVVTPVAPNKQMLKKRKSRLENVLITMKLKCLDAQTAQEQPIDLSYLLSGRGARQRKTQQSSSSSTANST

Target ORF information:

RefSeq Version	NM_079436.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster absent, small, or homeotic discs 1 (ash1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079436.3

ORF Insert Sequence:

ATGAGCTGTA GCCAAAATGA GACGGCAGCA GCAAAGGTTC TGGAAACGCA ACGCGCACAA 
GAATCCGGCA GTGAAAATGA GGAAACCGAT TCCATTACGG ATCAATCGAG CCAGTCGAAG 
TCGATCAAGT CAGCCACCCA GTTTAGCGTG CAGCGTTCGG ACACCGATGG ACTGCGAATG 
AGAATCTCGG CCATCCGCCC CACGTTAGGA GTCGTAGCAA CCAAGAAACC CCCAAAGTCC 
AGAAAAATGT CTACCCAGGA CACCGAGTCC GGCTGCTCGG AGGCCAAAAA TAGAGCGGTC 
AGCAAGAAAG TGAAGGTCAA GCGCAAGAAG CTGGCAAGTT CCAGTGGGAT TAGTAAATCG 
GACAAAGTGT CTAAGTCTAA GAAGTCACAG ATCTCGGCAT TTTCCTCGGA CTCAGAGGAC 
GATCTGCCCT TGAAGGTGCA TCAGCAGAGA GCTCCGCGAG TGCTGCTAAG TGCTATCATC 
CAGGCGGCAC AGTCGGCCAG CAAACCCACC CTCGATATCG GAATCTCGTC CAGCGACAAC 
GAGTTACCTA ACCTGGTGCA GGCGGCTATC AAGCGAGTGG AGAGCGATAC GGAGGACACA 
ACTGTGGAAG GAAGTTTCCG CAAAGCGGCC AAGGACAAGA ACCTACCCCA GTACCAGTCA 
ACTTTGCTGC AAGACTTCAT GGAGAAGACT CAGATGCTGG GGCAGACCGT CAATGCGAAG 
CTTGCGGAGG AGAAAGTGGC TAAAGCTAAG GAGGAGACCC TAGTCCAAAC AGCTGTTCCT 
CGAAAGCGCA GAGGTAGACC CAAAAAAGTG GTTCCCACAG TTCCTGCCCC AGGAAACTCT 
GGCCCTGCTA TAAACGAATC TGCCGATTCG GGTGTGATAA GCACCACTAG CACAACGCAG 
AGCACTACTC CCTCCCCGAA AATGCAAAAT GAGAATGCTG TGCCGACGGG ATCGCTGCCA 
ATCGCCTCCA GCAGCAAGCC TAAGATCGAC ATGGCGTATC TGGACAAGCG AATGTATGCC 
ACTGAGCGGG TGCTCTATCC ACCGCCCAGG AGTAAGCGAC GGCAGAACAA TAAAAAGACA 
GCCTGCAGTT CATCCAACAA AGAGGAACTT CAGCTTGATC CGCTGTGGCG AGAGATCGAC 
GTGAACAAGA AGTTTAGGCT GAGGAGTATG AGTGTGGGTG CGGCTAGTGG AACAGGAGCA 
AGCACCACTA TTTGCAGTAA GGTCTTGGCC GCTAAGAGCG GTTACGTCTC GGATTACGGA 
AGCGTACGAC ATCAGCGGAG CAGCCACAAC CACAACTCCG GTTACAAGTC CGATGCCAGC 
TGCAAGAGTC GATACAGCAC CAAGAGTTGT ATGAGTCGCA GGAGCAGGGC AAAGAGCTGC 
GGCTACCGGA GTGATTGCAA GGAATCTGGA AAGTCAGGCC TAAGGATGAG GCGGAAGCGC 
CGGGCTTCCA TGCTCTTGAA GAGCTCAGCA GACGATACTG TCGAGGACCA GGACATCCTT 
CAGCTAGCTG GATTATCTCT GGGCCAGAGC AGTGAGGAGA GCAACGAATA CATCAGTAAG 
CCGAGCCTTA AAAGCCTTCC CACGACAAGT GCCAGCAAGA AGTACGGCGA AATCAATCGC 
TATGTGACCA CCGGGCAGTA TTTCGGTCGA GGCGGTAGTT TGTCTGCCAC CAACCCGGAT 
AACTTCATTA GTAAAATGAT GAACCAGCGT AAAGAAACCC CGGCTCCCAG CAAATCGTCC 
TGCAAGATTA AATCTCGCCG CTCATCGGCA GCCAGTATGT GCAGCAGCTA TGTGTCTGGG 
GTGTCTAGAA TGCGTCGCAG ACATCGCCGG AAGAGTTTCA GTCACAACAA ATCATTGAAC 
ATTGATTCCA AGCTGCTCAC CGAAATCGAG ATAATCACAA GCACCTTCAA CTCAAGGTGT 
CGCATCCAAG ATGATCGTTT GACCGGAAGC AGTGGCAAAG AGAAGCTATT GGCCGATGCC 
AACAAGCTGC AGGCCACCCT AGCAGCACCA AGCCCTGCCC AGCAGTTGAC TCTGAATGGA 
GGAGGACCAG CTTCCACCCT TTCCAAACCA TTAAAACGGG GCCTAAAGAA GAGAAAGCTG 
AGTGAGCCCC TAGTGGACTT CGCTATGCTA TCAGCTAGCG CTAGTGGAAC TCCCAATGGC 
AGCGGAAGCA GTAATGGAAA TACCAAACGA CGACACAAGA AATCTCAAAG CAATGACAGT 
TCCAGTCCCG ATGATCACAA GTTGCCGTTG AAAAAGCGCC ACTATCTTTT GACTCCAGGA 
GAGCGTCCGC CGGCGGAAGT AGCATTCGCC AATGGGAAAT TGAACGCAGA GGCCTGGGCT 
GCAGCCGCGG CGGCTGCTAA GAGCACTGCG TCTACCAAAT CGCAAGCCCA GTTTAATGCT 
AGGAGCGTAA AATCTGCGCT AACGCCCAAA AAAAGACATC TCCTAGAGCA ACCTACGTCT 
GTGAGCGGAG CTGGCTCGTC GGCCAGTAAT TCTCCCCTGA GAATTGTTGT CGATAATAAT 
TCCATAAGTG GTGGAAAGTT GCTGGATATA AGTCCTAGTT CCTTGTGTTC CCTCAAACAG 
CAGAGGAGAG GAGGAGCAGC TAAACAGAAG GTGTCGGCAG CGAAGGACCT CGTTCAGCTT 
CAATCCCCAG CTGGTAGCTA TCCTCCCCCC GGTGTGTTCG AGCCATCTGT GGAGCTGGAA 
ATCCAAATTC CTCTTAGTAA ACTGAACGAA TCCGTCATAA CCAAAGCAGA GGTCGAATCT 
CCACTGCTCT CAGCATTGGA CATTAAGGAG GATACGAAAA AGGAGGTTGG CCAGCGCGTT 
GTCGAAACCT TGCTACACAA AACGGGAGGC AATCTTCTTC TGAAACGCAA GCGGAAAAAG 
ATAAATCGAA CTGGATTTCC CACTGTTCGC AGGAAGAAAC GTAAAGTTAG CGTGGAGCAG 
CAAACAACAG CCGTGATTGA TGAGCACGAG CCGGAGTTTG ATCCCGATGA TGAGCCACTG 
CAATCCCTAA GAGAGACCAG GAGTAGCAAT AATGTCAATG TGCAGGCGGC ACCTAATCCT 
CCACTGGATT GCGAGCGAGT TCCCCAAGCA GGCGAGGCCA GAGAAACCTT TGTGGCCAGG 
ACCAATCAAA AAGCCCCTCG ATTATCGGTG GTGGCCCTGG AGCGCCTACA GCGTCCTCAA 
ACACCAGCTA GAGGAAGACC GCGAGGTAGA AAACCTAAGA ACAGGGAACA AGCTGAAGCT 
GCACCTCAAC CGCCGCCCAA ATCGGAACCT GAGATAAGGC CAGCCAAAAA ACGTGGCCGG 
CAACCCAAGC AGCCGGTACT GGAAGAGCCA CCACCCACAC CACCTCCTCA ACAGAAAAAA 
AACAAAATGG AACCAAATAT TAGACTACCA GATGGCATCG ATCCCAATAC GAATTTCAGC 
TGCAAGATTC GCTTGAAGCG GCGAAAGAAC TTAGAGGCTG GAACCCAACC AAAAAAGGAG 
AAGCCAGTCC AGCCAGTGAC GGTGGAAGAG ATTCCACCAG AAATTCCCGT CAGTCAAGAA 
GAAATAGATG CAGAAGCAGA GGCTAAACGG CTAGACAGTA TTCCTACCGA GCACGATCCC 
TTGCCTGCCA GTGAGTCCCA CAACCCCGGT CCGCAGGACT ATGCCAGTTG CAGTGAATCC 
AGCGAGGACA AGGCATCAAC CACATCCTTG CGGAAACTAT CTAAGGTCAA GAAGACCTAT 
CTTGTAGCAG GACTCTTCTC GAATCATTAC AAACAATCCC TGATGCCGCC CCCAGCAAAG 
GTGAACAAAA AACCAGGCCT GGAGGAGCAA GTGGGTCCTG CGAGCCTTCT ACCACCACCT 
CCCTATTGCG AAAAGTACCT CCGAAGGACT GAAATGGACT TTGAGCTACC TTACGACATT 
TGGTGGGCTT ACACTAATTC CAAATTACCC ACTCGAAACG TTGTGCCATC CTGGAATTAC 
CGAAAGATCC GCACCAATGT ATACGCAGAG TCAGTGCGTC CAAATTTGGC GGGATTCGAT 
CATCCCACCT GTAACTGTAA GAACCAGGGC GAGAAGTCTT GTTTGGACAA CTGTCTCAAC 
CGCATGGTTT ATACGGAGTG CTCGCCCAGC AATTGCCCGG CTGGAGAGAA GTGCCGAAAT 
CAGAAGATCC AGCGACATGC CGTCGCACCC GGAGTGGAGC GCTTTATGAC GGCAGATAAA 
GGATGGGGAG TGCGAACTAA GCTACCCATT GCGAAGGGAA CCTACATTCT GGAGTACGTG 
GGCGAGGTAG TCACGGAAAA GGAATTCAAG CAGAGGATGG CCAGCATCTA CCTAAATGAC 
ACCCACCACT ATTGCTTACA TTTGGATGGA GGACTGGTTA TCGACGGTCA GCGGATGGGC 
AGCGATTGTA GGTTTGTCAA CCATTCTTGC GAACCCAATT GCGAGATGCA AAAATGGAGT 
GTTAACGGCC TATCTAGAAT GGTTTTATTC GCCAAAAGAG CCATAGAGGA GGGGGAGGAG 
CTGACATACG ATTACAACTT TTCGCTGTTC AATCCCTCAG AGGGTCAGCC CTGCAGGTGC 
AACACGCCCC AGTGTCGTGG TGTCATTGGT GGCAAGTCGC AGAGAGTTAA ACCCCTTCCT 
GCTGTGGAGG CCAAGCCATC TGGAGAGGGA CTTTCTGGCC GAAACGGGCG CCAACGGAAG 
CAGAAGGCCA AAAAGCATGC TCAACGGCAG GCGGGAAAAG ATATTTCATC AGCAGTGGCG 
GTGGCAAAGC TTCAACCATT GTCCGAAAAA GAAAAGAAAC TGGTCAGACA ATTCAACACA 
TTTCTAGTCA GGAACTTCGA AAAGATACGC AGATGCAAGG CCAAGCGGGC GTCAGATGCA 
GCGGCGACTG CATCCTCGCC AGCACTTGGC ACCACTAATG GGGACATTCC TGGTAGACGT 
CCCTCCACAC CATCTTCTCC TTCCTTGGCA GCGCAGATTT CCGCGCTCTG CTCGCCTCGC 
AACATAAAAA CCCGTGGACT CACACAGGCA GTGCATGATC CCGAACTAGA GAAAATGGCC 
AAAATGGCTG TAGTTCTAAG GGATATTTGC AGTGCCATGG AGACCCTCAA AATGTCCGAT 
TTGTTGACGA CAGTGTCCAG CAAGAAAAAG AAGCCTATAA AGACCACTTT GAGTGGAAAA 
TTGGGTTCTA CAGCTGCAAC TTCAAAAGTG GAATTCAGAT CGATACAAGC CCAGGTGGAG 
CAGGGACATT ACAAAACGCC GCAGGAATTC GATGACCACA TGCAGCAGCT CTTTGTGGAG 
GCCAAGCAGC AACACGGCGA TGATGAGGGC AAGGAAAAAG CGCTGCAGTC CCTGAAGGAT 
AGCTATGAGC AACAGAAGAT CGCCAGCTAT GTTCAGCTGG TGGAGATTCT TGGTGATTCG 
GAATCGTTGC AGAGCTTTAA ACCAAAGGAA GTGCTTTCGT CAGAGGAGGA ACCTGGAAAG 
ATAGCAGTGA AGAAATCACC AGGAGCAAAG GAAAGAGATT CCCCAATTGT GCCATTGAAA 
GTGACTCCGC CACCCCTGCT TCCTATTGAG GCCTCTCCTG ATGAGGATGT AATCCGATGT 
ATTTGTGGAT TGTACAAAGA TGAAGGCTTG ATGATTCAGT GCTCCAAATG CATGGTGTGG 
CAGCATACTG AATGTACCAA AGCTGACATC GATGCGGATA ATTATCAGTG CGAGCGTTGC 
GAGCCAAGGG AAGTGGATCG AGAAATTCCC CTGGAGGAAT TTACCGAAGA GGGACACCGT 
TACTATCTCT CCCTAATGCG TGGTGATCTG CAGGTACGAC AGGGCGATGC CGTCTATGTC 
CTACGAGACA TCCCCATCAA GGATGAGTCC GGCAAGGTGT TACCGACGAA GAAGCACACC 
TATGAAACGA TCGGAGCCAT TGATTACCAA GAGTGCGATA TCTTTCGGGT GGAGCACTTG 
TGGAAAAACG AACTGGGAAA GCGTTTTATA TTCGGACACC ATTTCCTGCG TCCTCATGAA 
ACTTTCCACG AACCATCGCG TCGTTTCTAC CCCAATGAGG TGGTACGAGT TTCACTTTAT 
GAGGTGGTAC CCATTGAGTT GGTCATTGGA CGCTGCTGGG TGTTGGATCG AACAACTTTC 
TGTAAAGGAC GCCCCATGGA ATGCAACGAT GAGGATCATT GCTACATCTG CGAGCTGCGT 
GTGGACAAGA CGGCAAGGTT CTTCTCAAAG GCCAAGGCCA ACCATCCAGC CTGCACCAAG 
AGCTATGCCT TCAGAAAATT TCCCGAGAAG ATTAAGATCT CCAAGAGCTA TGCGCCCCAT 
GATGTGGATC CGTCGTTGCT GAAGACAAGG AAGCAAAAGA CTGAATTAGA CGTAGGAGCC 
GGGCCAACAA CGATGCACAA GGTGTCTGGC AGGCAGGAAC AGCATCAGGC CAAGATGGTT 
GGGCGAAAGC CTCGTGGGAT CAGTGCCCCA GCGGATGCAA CTGCTGTCCA TGTCGTAACA 
CCCGTGGCGC CCAATAAACA GATGCTTAAG AAGAGGAAAT CGCGTTTAGA GAACGTTTTG 
ATAACTATGA AGCTTAAGTG TCTGGATGCA CAAACGGCAC AGGAGCAACC CATTGACTTG 
TCATACCTGC TGTCCGGACG CGGCGCCCGG CAGCGGAAGA CCCAGCAGTC CAGTAGCAGT 
TCTACGGCCA ACTCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash1 ( NM_001259905.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246834.1 Drosophila melanogaster absent, small, or homeotic discs 1 (ash1), transcript variant C, mRNA.

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CloneID	OFa18982
Clone ID Related Accession (Same CDS sequence)	NM_079436.3 , NM_001259905.1
Accession Version	NM_001259905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	absent, small, or homeotic discs 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGTA GCCAAAATGA GACGGCAGCA GCAAAGGTTC TGGAAACGCA ACGCGCACAA 
GAATCCGGCA GTGAAAATGA GGAAACCGAT TCCATTACGG ATCAATCGAG CCAGTCGAAG 
TCGATCAAGT CAGCCACCCA GTTTAGCGTG CAGCGTTCGG ACACCGATGG ACTGCGAATG 
AGAATCTCGG CCATCCGCCC CACGTTAGGA GTCGTAGCAA CCAAGAAACC CCCAAAGTCC 
AGAAAAATGT CTACCCAGGA CACCGAGTCC GGCTGCTCGG AGGCCAAAAA TAGAGCGGTC 
AGCAAGAAAG TGAAGGTCAA GCGCAAGAAG CTGGCAAGTT CCAGTGGGAT TAGTAAATCG 
GACAAAGTGT CTAAGTCTAA GAAGTCACAG ATCTCGGCAT TTTCCTCGGA CTCAGAGGAC 
GATCTGCCCT TGAAGGTGCA TCAGCAGAGA GCTCCGCGAG TGCTGCTAAG TGCTATCATC 
CAGGCGGCAC AGTCGGCCAG CAAACCCACC CTCGATATCG GAATCTCGTC CAGCGACAAC 
GAGTTACCTA ACCTGGTGCA GGCGGCTATC AAGCGAGTGG AGAGCGATAC GGAGGACACA 
ACTGTGGAAG GAAGTTTCCG CAAAGCGGCC AAGGACAAGA ACCTACCCCA GTACCAGTCA 
ACTTTGCTGC AAGACTTCAT GGAGAAGACT CAGATGCTGG GGCAGACCGT CAATGCGAAG 
CTTGCGGAGG AGAAAGTGGC TAAAGCTAAG GAGGAGACCC TAGTCCAAAC AGCTGTTCCT 
CGAAAGCGCA GAGGTAGACC CAAAAAAGTG GTTCCCACAG TTCCTGCCCC AGGAAACTCT 
GGCCCTGCTA TAAACGAATC TGCCGATTCG GGTGTGATAA GCACCACTAG CACAACGCAG 
AGCACTACTC CCTCCCCGAA AATGCAAAAT GAGAATGCTG TGCCGACGGG ATCGCTGCCA 
ATCGCCTCCA GCAGCAAGCC TAAGATCGAC ATGGCGTATC TGGACAAGCG AATGTATGCC 
ACTGAGCGGG TGCTCTATCC ACCGCCCAGG AGTAAGCGAC GGCAGAACAA TAAAAAGACA 
GCCTGCAGTT CATCCAACAA AGAGGAACTT CAGCTTGATC CGCTGTGGCG AGAGATCGAC 
GTGAACAAGA AGTTTAGGCT GAGGAGTATG AGTGTGGGTG CGGCTAGTGG AACAGGAGCA 
AGCACCACTA TTTGCAGTAA GGTCTTGGCC GCTAAGAGCG GTTACGTCTC GGATTACGGA 
AGCGTACGAC ATCAGCGGAG CAGCCACAAC CACAACTCCG GTTACAAGTC CGATGCCAGC 
TGCAAGAGTC GATACAGCAC CAAGAGTTGT ATGAGTCGCA GGAGCAGGGC AAAGAGCTGC 
GGCTACCGGA GTGATTGCAA GGAATCTGGA AAGTCAGGCC TAAGGATGAG GCGGAAGCGC 
CGGGCTTCCA TGCTCTTGAA GAGCTCAGCA GACGATACTG TCGAGGACCA GGACATCCTT 
CAGCTAGCTG GATTATCTCT GGGCCAGAGC AGTGAGGAGA GCAACGAATA CATCAGTAAG 
CCGAGCCTTA AAAGCCTTCC CACGACAAGT GCCAGCAAGA AGTACGGCGA AATCAATCGC 
TATGTGACCA CCGGGCAGTA TTTCGGTCGA GGCGGTAGTT TGTCTGCCAC CAACCCGGAT 
AACTTCATTA GTAAAATGAT GAACCAGCGT AAAGAAACCC CGGCTCCCAG CAAATCGTCC 
TGCAAGATTA AATCTCGCCG CTCATCGGCA GCCAGTATGT GCAGCAGCTA TGTGTCTGGG 
GTGTCTAGAA TGCGTCGCAG ACATCGCCGG AAGAGTTTCA GTCACAACAA ATCATTGAAC 
ATTGATTCCA AGCTGCTCAC CGAAATCGAG ATAATCACAA GCACCTTCAA CTCAAGGTGT 
CGCATCCAAG ATGATCGTTT GACCGGAAGC AGTGGCAAAG AGAAGCTATT GGCCGATGCC 
AACAAGCTGC AGGCCACCCT AGCAGCACCA AGCCCTGCCC AGCAGTTGAC TCTGAATGGA 
GGAGGACCAG CTTCCACCCT TTCCAAACCA TTAAAACGGG GCCTAAAGAA GAGAAAGCTG 
AGTGAGCCCC TAGTGGACTT CGCTATGCTA TCAGCTAGCG CTAGTGGAAC TCCCAATGGC 
AGCGGAAGCA GTAATGGAAA TACCAAACGA CGACACAAGA AATCTCAAAG CAATGACAGT 
TCCAGTCCCG ATGATCACAA GTTGCCGTTG AAAAAGCGCC ACTATCTTTT GACTCCAGGA 
GAGCGTCCGC CGGCGGAAGT AGCATTCGCC AATGGGAAAT TGAACGCAGA GGCCTGGGCT 
GCAGCCGCGG CGGCTGCTAA GAGCACTGCG TCTACCAAAT CGCAAGCCCA GTTTAATGCT 
AGGAGCGTAA AATCTGCGCT AACGCCCAAA AAAAGACATC TCCTAGAGCA ACCTACGTCT 
GTGAGCGGAG CTGGCTCGTC GGCCAGTAAT TCTCCCCTGA GAATTGTTGT CGATAATAAT 
TCCATAAGTG GTGGAAAGTT GCTGGATATA AGTCCTAGTT CCTTGTGTTC CCTCAAACAG 
CAGAGGAGAG GAGGAGCAGC TAAACAGAAG GTGTCGGCAG CGAAGGACCT CGTTCAGCTT 
CAATCCCCAG CTGGTAGCTA TCCTCCCCCC GGTGTGTTCG AGCCATCTGT GGAGCTGGAA 
ATCCAAATTC CTCTTAGTAA ACTGAACGAA TCCGTCATAA CCAAAGCAGA GGTCGAATCT 
CCACTGCTCT CAGCATTGGA CATTAAGGAG GATACGAAAA AGGAGGTTGG CCAGCGCGTT 
GTCGAAACCT TGCTACACAA AACGGGAGGC AATCTTCTTC TGAAACGCAA GCGGAAAAAG 
ATAAATCGAA CTGGATTTCC CACTGTTCGC AGGAAGAAAC GTAAAGTTAG CGTGGAGCAG 
CAAACAACAG CCGTGATTGA TGAGCACGAG CCGGAGTTTG ATCCCGATGA TGAGCCACTG 
CAATCCCTAA GAGAGACCAG GAGTAGCAAT AATGTCAATG TGCAGGCGGC ACCTAATCCT 
CCACTGGATT GCGAGCGAGT TCCCCAAGCA GGCGAGGCCA GAGAAACCTT TGTGGCCAGG 
ACCAATCAAA AAGCCCCTCG ATTATCGGTG GTGGCCCTGG AGCGCCTACA GCGTCCTCAA 
ACACCAGCTA GAGGAAGACC GCGAGGTAGA AAACCTAAGA ACAGGGAACA AGCTGAAGCT 
GCACCTCAAC CGCCGCCCAA ATCGGAACCT GAGATAAGGC CAGCCAAAAA ACGTGGCCGG 
CAACCCAAGC AGCCGGTACT GGAAGAGCCA CCACCCACAC CACCTCCTCA ACAGAAAAAA 
AACAAAATGG AACCAAATAT TAGACTACCA GATGGCATCG ATCCCAATAC GAATTTCAGC 
TGCAAGATTC GCTTGAAGCG GCGAAAGAAC TTAGAGGCTG GAACCCAACC AAAAAAGGAG 
AAGCCAGTCC AGCCAGTGAC GGTGGAAGAG ATTCCACCAG AAATTCCCGT CAGTCAAGAA 
GAAATAGATG CAGAAGCAGA GGCTAAACGG CTAGACAGTA TTCCTACCGA GCACGATCCC 
TTGCCTGCCA GTGAGTCCCA CAACCCCGGT CCGCAGGACT ATGCCAGTTG CAGTGAATCC 
AGCGAGGACA AGGCATCAAC CACATCCTTG CGGAAACTAT CTAAGGTCAA GAAGACCTAT 
CTTGTAGCAG GACTCTTCTC GAATCATTAC AAACAATCCC TGATGCCGCC CCCAGCAAAG 
GTGAACAAAA AACCAGGCCT GGAGGAGCAA GTGGGTCCTG CGAGCCTTCT ACCACCACCT 
CCCTATTGCG AAAAGTACCT CCGAAGGACT GAAATGGACT TTGAGCTACC TTACGACATT 
TGGTGGGCTT ACACTAATTC CAAATTACCC ACTCGAAACG TTGTGCCATC CTGGAATTAC 
CGAAAGATCC GCACCAATGT ATACGCAGAG TCAGTGCGTC CAAATTTGGC GGGATTCGAT 
CATCCCACCT GTAACTGTAA GAACCAGGGC GAGAAGTCTT GTTTGGACAA CTGTCTCAAC 
CGCATGGTTT ATACGGAGTG CTCGCCCAGC AATTGCCCGG CTGGAGAGAA GTGCCGAAAT 
CAGAAGATCC AGCGACATGC CGTCGCACCC GGAGTGGAGC GCTTTATGAC GGCAGATAAA 
GGATGGGGAG TGCGAACTAA GCTACCCATT GCGAAGGGAA CCTACATTCT GGAGTACGTG 
GGCGAGGTAG TCACGGAAAA GGAATTCAAG CAGAGGATGG CCAGCATCTA CCTAAATGAC 
ACCCACCACT ATTGCTTACA TTTGGATGGA GGACTGGTTA TCGACGGTCA GCGGATGGGC 
AGCGATTGTA GGTTTGTCAA CCATTCTTGC GAACCCAATT GCGAGATGCA AAAATGGAGT 
GTTAACGGCC TATCTAGAAT GGTTTTATTC GCCAAAAGAG CCATAGAGGA GGGGGAGGAG 
CTGACATACG ATTACAACTT TTCGCTGTTC AATCCCTCAG AGGGTCAGCC CTGCAGGTGC 
AACACGCCCC AGTGTCGTGG TGTCATTGGT GGCAAGTCGC AGAGAGTTAA ACCCCTTCCT 
GCTGTGGAGG CCAAGCCATC TGGAGAGGGA CTTTCTGGCC GAAACGGGCG CCAACGGAAG 
CAGAAGGCCA AAAAGCATGC TCAACGGCAG GCGGGAAAAG ATATTTCATC AGCAGTGGCG 
GTGGCAAAGC TTCAACCATT GTCCGAAAAA GAAAAGAAAC TGGTCAGACA ATTCAACACA 
TTTCTAGTCA GGAACTTCGA AAAGATACGC AGATGCAAGG CCAAGCGGGC GTCAGATGCA 
GCGGCGACTG CATCCTCGCC AGCACTTGGC ACCACTAATG GGGACATTCC TGGTAGACGT 
CCCTCCACAC CATCTTCTCC TTCCTTGGCA GCGCAGATTT CCGCGCTCTG CTCGCCTCGC 
AACATAAAAA CCCGTGGACT CACACAGGCA GTGCATGATC CCGAACTAGA GAAAATGGCC 
AAAATGGCTG TAGTTCTAAG GGATATTTGC AGTGCCATGG AGACCCTCAA AATGTCCGAT 
TTGTTGACGA CAGTGTCCAG CAAGAAAAAG AAGCCTATAA AGACCACTTT GAGTGGAAAA 
TTGGGTTCTA CAGCTGCAAC TTCAAAAGTG GAATTCAGAT CGATACAAGC CCAGGTGGAG 
CAGGGACATT ACAAAACGCC GCAGGAATTC GATGACCACA TGCAGCAGCT CTTTGTGGAG 
GCCAAGCAGC AACACGGCGA TGATGAGGGC AAGGAAAAAG CGCTGCAGTC CCTGAAGGAT 
AGCTATGAGC AACAGAAGAT CGCCAGCTAT GTTCAGCTGG TGGAGATTCT TGGTGATTCG 
GAATCGTTGC AGAGCTTTAA ACCAAAGGAA GTGCTTTCGT CAGAGGAGGA ACCTGGAAAG 
ATAGCAGTGA AGAAATCACC AGGAGCAAAG GAAAGAGATT CCCCAATTGT GCCATTGAAA 
GTGACTCCGC CACCCCTGCT TCCTATTGAG GCCTCTCCTG ATGAGGATGT AATCCGATGT 
ATTTGTGGAT TGTACAAAGA TGAAGGCTTG ATGATTCAGT GCTCCAAATG CATGGTGTGG 
CAGCATACTG AATGTACCAA AGCTGACATC GATGCGGATA ATTATCAGTG CGAGCGTTGC 
GAGCCAAGGG AAGTGGATCG AGAAATTCCC CTGGAGGAAT TTACCGAAGA GGGACACCGT 
TACTATCTCT CCCTAATGCG TGGTGATCTG CAGGTACGAC AGGGCGATGC CGTCTATGTC 
CTACGAGACA TCCCCATCAA GGATGAGTCC GGCAAGGTGT TACCGACGAA GAAGCACACC 
TATGAAACGA TCGGAGCCAT TGATTACCAA GAGTGCGATA TCTTTCGGGT GGAGCACTTG 
TGGAAAAACG AACTGGGAAA GCGTTTTATA TTCGGACACC ATTTCCTGCG TCCTCATGAA 
ACTTTCCACG AACCATCGCG TCGTTTCTAC CCCAATGAGG TGGTACGAGT TTCACTTTAT 
GAGGTGGTAC CCATTGAGTT GGTCATTGGA CGCTGCTGGG TGTTGGATCG AACAACTTTC 
TGTAAAGGAC GCCCCATGGA ATGCAACGAT GAGGATCATT GCTACATCTG CGAGCTGCGT 
GTGGACAAGA CGGCAAGGTT CTTCTCAAAG GCCAAGGCCA ACCATCCAGC CTGCACCAAG 
AGCTATGCCT TCAGAAAATT TCCCGAGAAG ATTAAGATCT CCAAGAGCTA TGCGCCCCAT 
GATGTGGATC CGTCGTTGCT GAAGACAAGG AAGCAAAAGA CTGAATTAGA CGTAGGAGCC 
GGGCCAACAA CGATGCACAA GGTGTCTGGC AGGCAGGAAC AGCATCAGGC CAAGATGGTT 
GGGCGAAAGC CTCGTGGGAT CAGTGCCCCA GCGGATGCAA CTGCTGTCCA TGTCGTAACA 
CCCGTGGCGC CCAATAAACA GATGCTTAAG AAGAGGAAAT CGCGTTTAGA GAACGTTTTG 
ATAACTATGA AGCTTAAGTG TCTGGATGCA CAAACGGCAC AGGAGCAACC CATTGACTTG 
TCATACCTGC TGTCCGGACG CGGCGCCCGG CAGCGGAAGA CCCAGCAGTC CAGTAGCAGT 
TCTACGGCCA ACTCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246834.1
CDS	406..7086
Translation	MSCSQNETAAAKVLETQRAQESGSENEETDSITDQSSQSKSIKSATQFSVQRSDTDGLRMRISAIRPTLGVVATKKPPKSRKMSTQDTESGCSEAKNRAVSKKVKVKRKKLASSSGISKSDKVSKSKKSQISAFSSDSEDDLPLKVHQQRAPRVLLSAIIQAAQSASKPTLDIGISSSDNELPNLVQAAIKRVESDTEDTTVEGSFRKAAKDKNLPQYQSTLLQDFMEKTQMLGQTVNAKLAEEKVAKAKEETLVQTAVPRKRRGRPKKVVPTVPAPGNSGPAINESADSGVISTTSTTQSTTPSPKMQNENAVPTGSLPIASSSKPKIDMAYLDKRMYATERVLYPPPRSKRRQNNKKTACSSSNKEELQLDPLWREIDVNKKFRLRSMSVGAASGTGASTTICSKVLAAKSGYVSDYGSVRHQRSSHNHNSGYKSDASCKSRYSTKSCMSRRSRAKSCGYRSDCKESGKSGLRMRRKRRASMLLKSSADDTVEDQDILQLAGLSLGQSSEESNEYISKPSLKSLPTTSASKKYGEINRYVTTGQYFGRGGSLSATNPDNFISKMMNQRKETPAPSKSSCKIKSRRSSAASMCSSYVSGVSRMRRRHRRKSFSHNKSLNIDSKLLTEIEIITSTFNSRCRIQDDRLTGSSGKEKLLADANKLQATLAAPSPAQQLTLNGGGPASTLSKPLKRGLKKRKLSEPLVDFAMLSASASGTPNGSGSSNGNTKRRHKKSQSNDSSSPDDHKLPLKKRHYLLTPGERPPAEVAFANGKLNAEAWAAAAAAAKSTASTKSQAQFNARSVKSALTPKKRHLLEQPTSVSGAGSSASNSPLRIVVDNNSISGGKLLDISPSSLCSLKQQRRGGAAKQKVSAAKDLVQLQSPAGSYPPPGVFEPSVELEIQIPLSKLNESVITKAEVESPLLSALDIKEDTKKEVGQRVVETLLHKTGGNLLLKRKRKKINRTGFPTVRRKKRKVSVEQQTTAVIDEHEPEFDPDDEPLQSLRETRSSNNVNVQAAPNPPLDCERVPQAGEARETFVARTNQKAPRLSVVALERLQRPQTPARGRPRGRKPKNREQAEAAPQPPPKSEPEIRPAKKRGRQPKQPVLEEPPPTPPPQQKKNKMEPNIRLPDGIDPNTNFSCKIRLKRRKNLEAGTQPKKEKPVQPVTVEEIPPEIPVSQEEIDAEAEAKRLDSIPTEHDPLPASESHNPGPQDYASCSESSEDKASTTSLRKLSKVKKTYLVAGLFSNHYKQSLMPPPAKVNKKPGLEEQVGPASLLPPPPYCEKYLRRTEMDFELPYDIWWAYTNSKLPTRNVVPSWNYRKIRTNVYAESVRPNLAGFDHPTCNCKNQGEKSCLDNCLNRMVYTECSPSNCPAGEKCRNQKIQRHAVAPGVERFMTADKGWGVRTKLPIAKGTYILEYVGEVVTEKEFKQRMASIYLNDTHHYCLHLDGGLVIDGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGEELTYDYNFSLFNPSEGQPCRCNTPQCRGVIGGKSQRVKPLPAVEAKPSGEGLSGRNGRQRKQKAKKHAQRQAGKDISSAVAVAKLQPLSEKEKKLVRQFNTFLVRNFEKIRRCKAKRASDAAATASSPALGTTNGDIPGRRPSTPSSPSLAAQISALCSPRNIKTRGLTQAVHDPELEKMAKMAVVLRDICSAMETLKMSDLLTTVSSKKKKPIKTTLSGKLGSTAATSKVEFRSIQAQVEQGHYKTPQEFDDHMQQLFVEAKQQHGDDEGKEKALQSLKDSYEQQKIASYVQLVEILGDSESLQSFKPKEVLSSEEEPGKIAVKKSPGAKERDSPIVPLKVTPPPLLPIEASPDEDVIRCICGLYKDEGLMIQCSKCMVWQHTECTKADIDADNYQCERCEPREVDREIPLEEFTEEGHRYYLSLMRGDLQVRQGDAVYVLRDIPIKDESGKVLPTKKHTYETIGAIDYQECDIFRVEHLWKNELGKRFIFGHHFLRPHETFHEPSRRFYPNEVVRVSLYEVVPIELVIGRCWVLDRTTFCKGRPMECNDEDHCYICELRVDKTARFFSKAKANHPACTKSYAFRKFPEKIKISKSYAPHDVDPSLLKTRKQKTELDVGAGPTTMHKVSGRQEQHQAKMVGRKPRGISAPADATAVHVVTPVAPNKQMLKKRKSRLENVLITMKLKCLDAQTAQEQPIDLSYLLSGRGARQRKTQQSSSSSTANST

Target ORF information:

RefSeq Version	NM_001259905.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster absent, small, or homeotic discs 1 (ash1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259905.1

ORF Insert Sequence:

ATGAGCTGTA GCCAAAATGA GACGGCAGCA GCAAAGGTTC TGGAAACGCA ACGCGCACAA 
GAATCCGGCA GTGAAAATGA GGAAACCGAT TCCATTACGG ATCAATCGAG CCAGTCGAAG 
TCGATCAAGT CAGCCACCCA GTTTAGCGTG CAGCGTTCGG ACACCGATGG ACTGCGAATG 
AGAATCTCGG CCATCCGCCC CACGTTAGGA GTCGTAGCAA CCAAGAAACC CCCAAAGTCC 
AGAAAAATGT CTACCCAGGA CACCGAGTCC GGCTGCTCGG AGGCCAAAAA TAGAGCGGTC 
AGCAAGAAAG TGAAGGTCAA GCGCAAGAAG CTGGCAAGTT CCAGTGGGAT TAGTAAATCG 
GACAAAGTGT CTAAGTCTAA GAAGTCACAG ATCTCGGCAT TTTCCTCGGA CTCAGAGGAC 
GATCTGCCCT TGAAGGTGCA TCAGCAGAGA GCTCCGCGAG TGCTGCTAAG TGCTATCATC 
CAGGCGGCAC AGTCGGCCAG CAAACCCACC CTCGATATCG GAATCTCGTC CAGCGACAAC 
GAGTTACCTA ACCTGGTGCA GGCGGCTATC AAGCGAGTGG AGAGCGATAC GGAGGACACA 
ACTGTGGAAG GAAGTTTCCG CAAAGCGGCC AAGGACAAGA ACCTACCCCA GTACCAGTCA 
ACTTTGCTGC AAGACTTCAT GGAGAAGACT CAGATGCTGG GGCAGACCGT CAATGCGAAG 
CTTGCGGAGG AGAAAGTGGC TAAAGCTAAG GAGGAGACCC TAGTCCAAAC AGCTGTTCCT 
CGAAAGCGCA GAGGTAGACC CAAAAAAGTG GTTCCCACAG TTCCTGCCCC AGGAAACTCT 
GGCCCTGCTA TAAACGAATC TGCCGATTCG GGTGTGATAA GCACCACTAG CACAACGCAG 
AGCACTACTC CCTCCCCGAA AATGCAAAAT GAGAATGCTG TGCCGACGGG ATCGCTGCCA 
ATCGCCTCCA GCAGCAAGCC TAAGATCGAC ATGGCGTATC TGGACAAGCG AATGTATGCC 
ACTGAGCGGG TGCTCTATCC ACCGCCCAGG AGTAAGCGAC GGCAGAACAA TAAAAAGACA 
GCCTGCAGTT CATCCAACAA AGAGGAACTT CAGCTTGATC CGCTGTGGCG AGAGATCGAC 
GTGAACAAGA AGTTTAGGCT GAGGAGTATG AGTGTGGGTG CGGCTAGTGG AACAGGAGCA 
AGCACCACTA TTTGCAGTAA GGTCTTGGCC GCTAAGAGCG GTTACGTCTC GGATTACGGA 
AGCGTACGAC ATCAGCGGAG CAGCCACAAC CACAACTCCG GTTACAAGTC CGATGCCAGC 
TGCAAGAGTC GATACAGCAC CAAGAGTTGT ATGAGTCGCA GGAGCAGGGC AAAGAGCTGC 
GGCTACCGGA GTGATTGCAA GGAATCTGGA AAGTCAGGCC TAAGGATGAG GCGGAAGCGC 
CGGGCTTCCA TGCTCTTGAA GAGCTCAGCA GACGATACTG TCGAGGACCA GGACATCCTT 
CAGCTAGCTG GATTATCTCT GGGCCAGAGC AGTGAGGAGA GCAACGAATA CATCAGTAAG 
CCGAGCCTTA AAAGCCTTCC CACGACAAGT GCCAGCAAGA AGTACGGCGA AATCAATCGC 
TATGTGACCA CCGGGCAGTA TTTCGGTCGA GGCGGTAGTT TGTCTGCCAC CAACCCGGAT 
AACTTCATTA GTAAAATGAT GAACCAGCGT AAAGAAACCC CGGCTCCCAG CAAATCGTCC 
TGCAAGATTA AATCTCGCCG CTCATCGGCA GCCAGTATGT GCAGCAGCTA TGTGTCTGGG 
GTGTCTAGAA TGCGTCGCAG ACATCGCCGG AAGAGTTTCA GTCACAACAA ATCATTGAAC 
ATTGATTCCA AGCTGCTCAC CGAAATCGAG ATAATCACAA GCACCTTCAA CTCAAGGTGT 
CGCATCCAAG ATGATCGTTT GACCGGAAGC AGTGGCAAAG AGAAGCTATT GGCCGATGCC 
AACAAGCTGC AGGCCACCCT AGCAGCACCA AGCCCTGCCC AGCAGTTGAC TCTGAATGGA 
GGAGGACCAG CTTCCACCCT TTCCAAACCA TTAAAACGGG GCCTAAAGAA GAGAAAGCTG 
AGTGAGCCCC TAGTGGACTT CGCTATGCTA TCAGCTAGCG CTAGTGGAAC TCCCAATGGC 
AGCGGAAGCA GTAATGGAAA TACCAAACGA CGACACAAGA AATCTCAAAG CAATGACAGT 
TCCAGTCCCG ATGATCACAA GTTGCCGTTG AAAAAGCGCC ACTATCTTTT GACTCCAGGA 
GAGCGTCCGC CGGCGGAAGT AGCATTCGCC AATGGGAAAT TGAACGCAGA GGCCTGGGCT 
GCAGCCGCGG CGGCTGCTAA GAGCACTGCG TCTACCAAAT CGCAAGCCCA GTTTAATGCT 
AGGAGCGTAA AATCTGCGCT AACGCCCAAA AAAAGACATC TCCTAGAGCA ACCTACGTCT 
GTGAGCGGAG CTGGCTCGTC GGCCAGTAAT TCTCCCCTGA GAATTGTTGT CGATAATAAT 
TCCATAAGTG GTGGAAAGTT GCTGGATATA AGTCCTAGTT CCTTGTGTTC CCTCAAACAG 
CAGAGGAGAG GAGGAGCAGC TAAACAGAAG GTGTCGGCAG CGAAGGACCT CGTTCAGCTT 
CAATCCCCAG CTGGTAGCTA TCCTCCCCCC GGTGTGTTCG AGCCATCTGT GGAGCTGGAA 
ATCCAAATTC CTCTTAGTAA ACTGAACGAA TCCGTCATAA CCAAAGCAGA GGTCGAATCT 
CCACTGCTCT CAGCATTGGA CATTAAGGAG GATACGAAAA AGGAGGTTGG CCAGCGCGTT 
GTCGAAACCT TGCTACACAA AACGGGAGGC AATCTTCTTC TGAAACGCAA GCGGAAAAAG 
ATAAATCGAA CTGGATTTCC CACTGTTCGC AGGAAGAAAC GTAAAGTTAG CGTGGAGCAG 
CAAACAACAG CCGTGATTGA TGAGCACGAG CCGGAGTTTG ATCCCGATGA TGAGCCACTG 
CAATCCCTAA GAGAGACCAG GAGTAGCAAT AATGTCAATG TGCAGGCGGC ACCTAATCCT 
CCACTGGATT GCGAGCGAGT TCCCCAAGCA GGCGAGGCCA GAGAAACCTT TGTGGCCAGG 
ACCAATCAAA AAGCCCCTCG ATTATCGGTG GTGGCCCTGG AGCGCCTACA GCGTCCTCAA 
ACACCAGCTA GAGGAAGACC GCGAGGTAGA AAACCTAAGA ACAGGGAACA AGCTGAAGCT 
GCACCTCAAC CGCCGCCCAA ATCGGAACCT GAGATAAGGC CAGCCAAAAA ACGTGGCCGG 
CAACCCAAGC AGCCGGTACT GGAAGAGCCA CCACCCACAC CACCTCCTCA ACAGAAAAAA 
AACAAAATGG AACCAAATAT TAGACTACCA GATGGCATCG ATCCCAATAC GAATTTCAGC 
TGCAAGATTC GCTTGAAGCG GCGAAAGAAC TTAGAGGCTG GAACCCAACC AAAAAAGGAG 
AAGCCAGTCC AGCCAGTGAC GGTGGAAGAG ATTCCACCAG AAATTCCCGT CAGTCAAGAA 
GAAATAGATG CAGAAGCAGA GGCTAAACGG CTAGACAGTA TTCCTACCGA GCACGATCCC 
TTGCCTGCCA GTGAGTCCCA CAACCCCGGT CCGCAGGACT ATGCCAGTTG CAGTGAATCC 
AGCGAGGACA AGGCATCAAC CACATCCTTG CGGAAACTAT CTAAGGTCAA GAAGACCTAT 
CTTGTAGCAG GACTCTTCTC GAATCATTAC AAACAATCCC TGATGCCGCC CCCAGCAAAG 
GTGAACAAAA AACCAGGCCT GGAGGAGCAA GTGGGTCCTG CGAGCCTTCT ACCACCACCT 
CCCTATTGCG AAAAGTACCT CCGAAGGACT GAAATGGACT TTGAGCTACC TTACGACATT 
TGGTGGGCTT ACACTAATTC CAAATTACCC ACTCGAAACG TTGTGCCATC CTGGAATTAC 
CGAAAGATCC GCACCAATGT ATACGCAGAG TCAGTGCGTC CAAATTTGGC GGGATTCGAT 
CATCCCACCT GTAACTGTAA GAACCAGGGC GAGAAGTCTT GTTTGGACAA CTGTCTCAAC 
CGCATGGTTT ATACGGAGTG CTCGCCCAGC AATTGCCCGG CTGGAGAGAA GTGCCGAAAT 
CAGAAGATCC AGCGACATGC CGTCGCACCC GGAGTGGAGC GCTTTATGAC GGCAGATAAA 
GGATGGGGAG TGCGAACTAA GCTACCCATT GCGAAGGGAA CCTACATTCT GGAGTACGTG 
GGCGAGGTAG TCACGGAAAA GGAATTCAAG CAGAGGATGG CCAGCATCTA CCTAAATGAC 
ACCCACCACT ATTGCTTACA TTTGGATGGA GGACTGGTTA TCGACGGTCA GCGGATGGGC 
AGCGATTGTA GGTTTGTCAA CCATTCTTGC GAACCCAATT GCGAGATGCA AAAATGGAGT 
GTTAACGGCC TATCTAGAAT GGTTTTATTC GCCAAAAGAG CCATAGAGGA GGGGGAGGAG 
CTGACATACG ATTACAACTT TTCGCTGTTC AATCCCTCAG AGGGTCAGCC CTGCAGGTGC 
AACACGCCCC AGTGTCGTGG TGTCATTGGT GGCAAGTCGC AGAGAGTTAA ACCCCTTCCT 
GCTGTGGAGG CCAAGCCATC TGGAGAGGGA CTTTCTGGCC GAAACGGGCG CCAACGGAAG 
CAGAAGGCCA AAAAGCATGC TCAACGGCAG GCGGGAAAAG ATATTTCATC AGCAGTGGCG 
GTGGCAAAGC TTCAACCATT GTCCGAAAAA GAAAAGAAAC TGGTCAGACA ATTCAACACA 
TTTCTAGTCA GGAACTTCGA AAAGATACGC AGATGCAAGG CCAAGCGGGC GTCAGATGCA 
GCGGCGACTG CATCCTCGCC AGCACTTGGC ACCACTAATG GGGACATTCC TGGTAGACGT 
CCCTCCACAC CATCTTCTCC TTCCTTGGCA GCGCAGATTT CCGCGCTCTG CTCGCCTCGC 
AACATAAAAA CCCGTGGACT CACACAGGCA GTGCATGATC CCGAACTAGA GAAAATGGCC 
AAAATGGCTG TAGTTCTAAG GGATATTTGC AGTGCCATGG AGACCCTCAA AATGTCCGAT 
TTGTTGACGA CAGTGTCCAG CAAGAAAAAG AAGCCTATAA AGACCACTTT GAGTGGAAAA 
TTGGGTTCTA CAGCTGCAAC TTCAAAAGTG GAATTCAGAT CGATACAAGC CCAGGTGGAG 
CAGGGACATT ACAAAACGCC GCAGGAATTC GATGACCACA TGCAGCAGCT CTTTGTGGAG 
GCCAAGCAGC AACACGGCGA TGATGAGGGC AAGGAAAAAG CGCTGCAGTC CCTGAAGGAT 
AGCTATGAGC AACAGAAGAT CGCCAGCTAT GTTCAGCTGG TGGAGATTCT TGGTGATTCG 
GAATCGTTGC AGAGCTTTAA ACCAAAGGAA GTGCTTTCGT CAGAGGAGGA ACCTGGAAAG 
ATAGCAGTGA AGAAATCACC AGGAGCAAAG GAAAGAGATT CCCCAATTGT GCCATTGAAA 
GTGACTCCGC CACCCCTGCT TCCTATTGAG GCCTCTCCTG ATGAGGATGT AATCCGATGT 
ATTTGTGGAT TGTACAAAGA TGAAGGCTTG ATGATTCAGT GCTCCAAATG CATGGTGTGG 
CAGCATACTG AATGTACCAA AGCTGACATC GATGCGGATA ATTATCAGTG CGAGCGTTGC 
GAGCCAAGGG AAGTGGATCG AGAAATTCCC CTGGAGGAAT TTACCGAAGA GGGACACCGT 
TACTATCTCT CCCTAATGCG TGGTGATCTG CAGGTACGAC AGGGCGATGC CGTCTATGTC 
CTACGAGACA TCCCCATCAA GGATGAGTCC GGCAAGGTGT TACCGACGAA GAAGCACACC 
TATGAAACGA TCGGAGCCAT TGATTACCAA GAGTGCGATA TCTTTCGGGT GGAGCACTTG 
TGGAAAAACG AACTGGGAAA GCGTTTTATA TTCGGACACC ATTTCCTGCG TCCTCATGAA 
ACTTTCCACG AACCATCGCG TCGTTTCTAC CCCAATGAGG TGGTACGAGT TTCACTTTAT 
GAGGTGGTAC CCATTGAGTT GGTCATTGGA CGCTGCTGGG TGTTGGATCG AACAACTTTC 
TGTAAAGGAC GCCCCATGGA ATGCAACGAT GAGGATCATT GCTACATCTG CGAGCTGCGT 
GTGGACAAGA CGGCAAGGTT CTTCTCAAAG GCCAAGGCCA ACCATCCAGC CTGCACCAAG 
AGCTATGCCT TCAGAAAATT TCCCGAGAAG ATTAAGATCT CCAAGAGCTA TGCGCCCCAT 
GATGTGGATC CGTCGTTGCT GAAGACAAGG AAGCAAAAGA CTGAATTAGA CGTAGGAGCC 
GGGCCAACAA CGATGCACAA GGTGTCTGGC AGGCAGGAAC AGCATCAGGC CAAGATGGTT 
GGGCGAAAGC CTCGTGGGAT CAGTGCCCCA GCGGATGCAA CTGCTGTCCA TGTCGTAACA 
CCCGTGGCGC CCAATAAACA GATGCTTAAG AAGAGGAAAT CGCGTTTAGA GAACGTTTTG 
ATAACTATGA AGCTTAAGTG TCTGGATGCA CAAACGGCAC AGGAGCAACC CATTGACTTG 
TCATACCTGC TGTCCGGACG CGGCGCCCGG CAGCGGAAGA CCCAGCAGTC CAGTAGCAGT 
TCTACGGCCA ACTCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ash1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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