Cpr76Bd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpr76Bd
Entrez Gene ID	40123
Full Name	Cuticular protein 76Bd
Synonyms	CG9299,DmelCpr76Bd,Dmel\CG9299
General protein information	Preferred Names Cuticular protein 76Bd Names CG9299 gene product from transcript CG9299-RC CG9299-PB CG9299-PC CG9299-PD Cpr76Bd-PB Cpr76Bd-PC Cpr76Bd-PD cuticular protein 76Bd
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76B6-76B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275124.1	NP_001262053.1	cuticular protein 76Bd, isoform D
NM_001104175.3	NP_001097645.1	cuticular protein 76Bd, isoform B
NM_001275123.1	NP_001262052.1	cuticular protein 76Bd, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	CPR140	XP_565330.3
Xenopus tropicalis (tropical clawed frog)	LOC101734022	XP_004920960.1
Drosophila melanogaster (fruit fly)	Cpr76Bd	NP_001262052.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cpr76Bd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpr76Bd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18972	NM_001104175.3 Latest version!	Drosophila melanogaster cuticular protein 76Bd, transcript variant B (Cpr76Bd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa18972	NM_001275124.1 Latest version!	Drosophila melanogaster cuticular protein 76Bd, transcript variant D (Cpr76Bd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21085	NM_001275123.1 Latest version!	Drosophila melanogaster cuticular protein 76Bd, transcript variant C (Cpr76Bd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpr76Bd ( NM_001275124.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262053.1 Drosophila melanogaster cuticular protein 76Bd, transcript variant D (Cpr76Bd), mRNA.

Price & Availability↑

CloneID	OFa18972
Clone ID Related Accession (Same CDS sequence)	NM_001275124.1 , NM_001104175.3
Accession Version	NM_001275124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cuticular protein 76Bd, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTTC ATCTGGTGGC CCTCCTGGGC ATACTCAGCG TGGTACTTGC GGATCCGTCG 
CCCACCTTGG CTCCACCAAT CTTGCCCTGC GTACACGCAT CCACTGCTGG TTATGCCTAT 
CCTCCACCGG CGGAGGTAAA GTTCTCCATC TCGCCCGGAG TGACGAAGTA CAGCCAGAGC 
CCGGCGATAT CCACGTACTC GGAGAACGGA CAACTCCTGC ACACCTCGGT GGGAAGTTCC 
GGAGCCGCCT ATCAGTCTAC GGCCGGAATC GAGGGCTTGT CTCACGGTGG CATAACTCAG 
ATCAACAAAT ACATAGCTCC CGTGCAGAAG AGCCTGTTCT CTCCCTCAGC GGAGTACGGA 
GGAGCGGGAA TTACCTATGC GGACAAGTCA CCAGCGGCCA AGTACGCAAC CGTTGTGCCG 
TCGGGCATCG AAATCAAGAA CCTGGTGTCG CCGGCGGTTA CCAAGTTGGA CAACTCCTAC 
TTGCCACCAT CACCCGGAAT CACTAAAGTG GCGACATACA CCTCGCCCGG ATATAGTTAC 
AGTTCGGCAA GTCCGGGAAT ATCCAAAGTG GCGACTTACT CCTCCCCATC GCTTTCCAGC 
GTTCCCTACT ATGCGCCACC AGTGACTTCC AAGGTGGAGA CATACAGCTC ACCTGCGTAC 
ACGTACAGCA AGACCACACC CGGCTACTCC AAGGTGGAGA CCTACAGCTC TCCTGGTTAC 
AGCTATGGAC AGATCTCGCC GGGCATCTCC AGGATAGCCA CCTACTCGCC ATCGGTTTCG 
TATTCAGCGC CAACGATTGC CAAGGTGTCC ACCTACTCAG CGCCTTCAGT GAAACTGGCC 
ACCACTTCGT CACTGCTCTC CTCCCACGGA ACAGGATACT CCGCCAGCTA TGCTCCCTCA 
ATTACCAAGT ACAGCCAGGT ATCAGATGTA TCCCACCAGT ACATATCCAA GCCTATAGTG 
GCAGCCTATC CGGCGATCAC CAAAGTGGCG GCCAGCTACG GAGGCACTGC ATCCGGAGCG 
CTGTCCCACC AGTATGTCTC CCAACCAGCC ATTGCCAAGG TGTCCACTTA CGCTGCTCCC 
ACGGTTGCAA CTTACTCATC TGGGCCTGCC ATCTCGAAGC TCTCCACCAG CTATGGAGCT 
TCAGGATCGG GAGCCGTCTC CCACCAATAT GTCTCGAAGC CAGCTGTGGC TATTGCCGCT 
CCAGCAGTGG CCAAGGTGGC AACGTATGCA GCTCCTGCCA TTTCAAGCTA CTCCACTGGT 
CCTGCCATTT CGAAGGTAGC ATCCTACGCA GCTCCCACTG TCTCCACCTA CTCCTCGGGA 
TATGGTTATG GTTCGAGTGG GTCAGGGGCC GTATCGCACC AGTACGTGTC CAAGCCTGCG 
GTGGCCATTT CAGCCGCTCC AGCCATCGCC AAGGTGGCTA CCTATGCAGC TCCCGCCATT 
TCAACTTATG CGGCAGCTCC AGTGGTGACC AAAGTCGCCA CTGGATATGG AGGAAGTGGC 
TCGGGATACA GTTCGGGCGC TGTTTCCCAT CAATATGTCT CTAAGCCAGC GGTTGCTAAA 
GTGGCAACCT ATGCTGCTCC TGCCATTTCA ACATACTCAG CAGCTCCGGC AGTGACCAAG 
ATCGCGACAA GCTATGGAGG ATCGGGTCAT GGAGCCGTCT CCCATCAGTA TGTCTCCAAG 
CCAGCAGTAG CCATCTCAGC AGCCCCCGCA ATCGCTAAGG TAGCGACATA CGCTTCTCCG 
GCAATATCAA CGTATGCCAC TGCTCCTGTG GTCTCAAAAG TGGCTACCTA CGCAGCTCCC 
TCCATAGCCA CTTACTCCTC CGCTCCGGCT CTAGCCAAGG TTTCCTATAG TCAGGCAGCG 
GATGTATCCC ACCAGTACAT TTCCAAACCT ATTGTAGCTG CCTATCCGGC AGCTGCTCCG 
GCAGTGGCCA CATACTCTTC CGCTCCTGCC ATCACCAAGC TCTCCACAAG CTATGAATCG 
TCAGGATCGG GAGCCGTCTC CCACCAGTAT GTGTCTAAAC CGGCGGTGGC TAAGGTCGCC 
GCATATGCAG CTCCAGCAAT CTCAACGTAT GCTACTGCTC CCGCCGTGTC CACTTACTCG 
TCAGCTCCAG TGGTAGCCAA AGTGGCCACT GGATACGGCG GAAGCGGATC GGGATACAGC 
TCCGGAGCAG TTTCACATCA ATATATCTCG AAGCCAGCAG TTGCCATTGC ATCAGCTCCA 
GCGATTGCCA AGGTAGCAAC ATATGCTGCT CCGGCCATAT CCGCCTATTC CTCGGCTCCC 
AGTCTCACCA AGATAGCCAC GGGCTATGGA GAATCGGCCC ATGGAGGAGC ACTCTCCCAC 
CAGTACATCT CTAAGCCGGC GATCGCAGTG GCTCCAGTGG CTCCTGTACT CTCCAAAACT 
TACTTGCCTG CCGCTCCAGC CGTCGCTAAA GTAGCTACAG GATATGGAAT AAGTGGTTCG 
GGAGCCGTGT CCCATCAGTA CGTATCCAAG CCAGCACTGA CACTTGCTGC GCCAGCGGTT 
GCCAAGGTGG CCACCTATGC TGCTCCGGCA ATATCCAGCT ACTCCACTGG AGCTGGCATA 
TCGAAAGGAT TGTCCTATGC AGCTCCTGTG GTTTCGACGG GCCACGGGTA CGGAGGAAGT 
GCCTCAGAAT ATAGTTCTGG AGCAGTCTCT CATCAGTATG TGTCCAAGCC AGCGGTTGCC 
ATTTCAGCTG CTCCTGCAAT TGCCAAAGTA GCAACGTATG TAGCTCCAGC TGTGGGTCAC 
ATTTTAGGTG GGCACGCCGT CGGCTCTGTT CCCGTTTTAT CCACTAAGCT GGCTACTGGC 
TACGGGGGCA GTGGATCGGG ATATAGCTCG GGAGCCGTTT CCCATCAATA TGTCTCCAAG 
CCAGCTGTGG CCATTTCAGC TGCTCCTGCT ATCGCCAAGG TGGCCGTTCC AACTGTCAGC 
CACATTTCCA CAGGTCCATC GACCCCGCTG GGAGTGGGAG TCGGGTACGG GTCTAGTGGA 
CATGGTGGAG TTTTGCTGGG TGCGCCGCTG ACGAAGTTGA CATCGGCTCC TGCTTATGCC 
CTGGGAGGAA AGCTGGCCAG CAGCACGGCC TACGGAATCC AAGCTGGGCA TGGATCTGGT 
CCCAGCGGTG GCTACTATGG TGCCATCTCC TTGGGTCATG CGAAGGTATC CCCGGCCCTT 
AGCTACCACG GTCTACTGTC CCATGGATCG GGTCTGGCAT CAGGCGCATC CTCTCTGGCC 
CATTTGGACT CCTCCCTGAG TGGATACTCA CACGGAGTGG GCGGAATCGG ACCGCTGGGA 
GCTGGATTCT ACCGCTATGC TCCAAGTGTG CCAGCGCTGA GCAGCCACGC CCCCGTGGCA 
GCCACCGCCT ACTTGAAGTC AGCACCCGTA ACTCAACATG CCGTCCTGAA GGTGGTGCCC 
GAGAAGCATC TGGAGCACTT CGATGCCCAT CCTCGATATG CCTTCGAGTA TGCCGTGAAT 
GATCCCCATA CGGGAGATAA CAAGCACCAG AAGGAGGAGC GAGATGGAGA CGTGGTCAAG 
GGAGAGTACT CCTTGGTTGA GCCCGATGGC AATGTGCGCA CGGTGAAGTA CTACGCCGAC 
TGGGAGACGG GCTTCCACGC CGAGGTCATC AACAGTCGCG ACCAGGGCAA AATTGTGGCC 
AAGCGTCAGA CGGAAACCAA GTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262053.1
CDS	348..4034
Translation	MKLHLVALLGILSVVLADPSPTLAPPILPCVHASTAGYAYPPPAEVKFSISPGVTKYSQSPAISTYSENGQLLHTSVGSSGAAYQSTAGIEGLSHGGITQINKYIAPVQKSLFSPSAEYGGAGITYADKSPAAKYATVVPSGIEIKNLVSPAVTKLDNSYLPPSPGITKVATYTSPGYSYSSASPGISKVATYSSPSLSSVPYYAPPVTSKVETYSSPAYTYSKTTPGYSKVETYSSPGYSYGQISPGISRIATYSPSVSYSAPTIAKVSTYSAPSVKLATTSSLLSSHGTGYSASYAPSITKYSQVSDVSHQYISKPIVAAYPAITKVAASYGGTASGALSHQYVSQPAIAKVSTYAAPTVATYSSGPAISKLSTSYGASGSGAVSHQYVSKPAVAIAAPAVAKVATYAAPAISSYSTGPAISKVASYAAPTVSTYSSGYGYGSSGSGAVSHQYVSKPAVAISAAPAIAKVATYAAPAISTYAAAPVVTKVATGYGGSGSGYSSGAVSHQYVSKPAVAKVATYAAPAISTYSAAPAVTKIATSYGGSGHGAVSHQYVSKPAVAISAAPAIAKVATYASPAISTYATAPVVSKVATYAAPSIATYSSAPALAKVSYSQAADVSHQYISKPIVAAYPAAAPAVATYSSAPAITKLSTSYESSGSGAVSHQYVSKPAVAKVAAYAAPAISTYATAPAVSTYSSAPVVAKVATGYGGSGSGYSSGAVSHQYISKPAVAIASAPAIAKVATYAAPAISAYSSAPSLTKIATGYGESAHGGALSHQYISKPAIAVAPVAPVLSKTYLPAAPAVAKVATGYGISGSGAVSHQYVSKPALTLAAPAVAKVATYAAPAISSYSTGAGISKGLSYAAPVVSTGHGYGGSASEYSSGAVSHQYVSKPAVAISAAPAIAKVATYVAPAVGHILGGHAVGSVPVLSTKLATGYGGSGSGYSSGAVSHQYVSKPAVAISAAPAIAKVAVPTVSHISTGPSTPLGVGVGYGSSGHGGVLLGAPLTKLTSAPAYALGGKLASSTAYGIQAGHGSGPSGGYYGAISLGHAKVSPALSYHGLLSHGSGLASGASSLAHLDSSLSGYSHGVGGIGPLGAGFYRYAPSVPALSSHAPVAATAYLKSAPVTQHAVLKVVPEKHLEHFDAHPRYAFEYAVNDPHTGDNKHQKEERDGDVVKGEYSLVEPDGNVRTVKYYADWETGFHAEVINSRDQGKIVAKRQTETKS

Target ORF information:

RefSeq Version	NM_001275124.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cuticular protein 76Bd, transcript variant D (Cpr76Bd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275124.1

ORF Insert Sequence:

ATGAAGCTTC ATCTGGTGGC CCTCCTGGGC ATACTCAGCG TGGTACTTGC GGATCCGTCG 
CCCACCTTGG CTCCACCAAT CTTGCCCTGC GTACACGCAT CCACTGCTGG TTATGCCTAT 
CCTCCACCGG CGGAGGTAAA GTTCTCCATC TCGCCCGGAG TGACGAAGTA CAGCCAGAGC 
CCGGCGATAT CCACGTACTC GGAGAACGGA CAACTCCTGC ACACCTCGGT GGGAAGTTCC 
GGAGCCGCCT ATCAGTCTAC GGCCGGAATC GAGGGCTTGT CTCACGGTGG CATAACTCAG 
ATCAACAAAT ACATAGCTCC CGTGCAGAAG AGCCTGTTCT CTCCCTCAGC GGAGTACGGA 
GGAGCGGGAA TTACCTATGC GGACAAGTCA CCAGCGGCCA AGTACGCAAC CGTTGTGCCG 
TCGGGCATCG AAATCAAGAA CCTGGTGTCG CCGGCGGTTA CCAAGTTGGA CAACTCCTAC 
TTGCCACCAT CACCCGGAAT CACTAAAGTG GCGACATACA CCTCGCCCGG ATATAGTTAC 
AGTTCGGCAA GTCCGGGAAT ATCCAAAGTG GCGACTTACT CCTCCCCATC GCTTTCCAGC 
GTTCCCTACT ATGCGCCACC AGTGACTTCC AAGGTGGAGA CATACAGCTC ACCTGCGTAC 
ACGTACAGCA AGACCACACC CGGCTACTCC AAGGTGGAGA CCTACAGCTC TCCTGGTTAC 
AGCTATGGAC AGATCTCGCC GGGCATCTCC AGGATAGCCA CCTACTCGCC ATCGGTTTCG 
TATTCAGCGC CAACGATTGC CAAGGTGTCC ACCTACTCAG CGCCTTCAGT GAAACTGGCC 
ACCACTTCGT CACTGCTCTC CTCCCACGGA ACAGGATACT CCGCCAGCTA TGCTCCCTCA 
ATTACCAAGT ACAGCCAGGT ATCAGATGTA TCCCACCAGT ACATATCCAA GCCTATAGTG 
GCAGCCTATC CGGCGATCAC CAAAGTGGCG GCCAGCTACG GAGGCACTGC ATCCGGAGCG 
CTGTCCCACC AGTATGTCTC CCAACCAGCC ATTGCCAAGG TGTCCACTTA CGCTGCTCCC 
ACGGTTGCAA CTTACTCATC TGGGCCTGCC ATCTCGAAGC TCTCCACCAG CTATGGAGCT 
TCAGGATCGG GAGCCGTCTC CCACCAATAT GTCTCGAAGC CAGCTGTGGC TATTGCCGCT 
CCAGCAGTGG CCAAGGTGGC AACGTATGCA GCTCCTGCCA TTTCAAGCTA CTCCACTGGT 
CCTGCCATTT CGAAGGTAGC ATCCTACGCA GCTCCCACTG TCTCCACCTA CTCCTCGGGA 
TATGGTTATG GTTCGAGTGG GTCAGGGGCC GTATCGCACC AGTACGTGTC CAAGCCTGCG 
GTGGCCATTT CAGCCGCTCC AGCCATCGCC AAGGTGGCTA CCTATGCAGC TCCCGCCATT 
TCAACTTATG CGGCAGCTCC AGTGGTGACC AAAGTCGCCA CTGGATATGG AGGAAGTGGC 
TCGGGATACA GTTCGGGCGC TGTTTCCCAT CAATATGTCT CTAAGCCAGC GGTTGCTAAA 
GTGGCAACCT ATGCTGCTCC TGCCATTTCA ACATACTCAG CAGCTCCGGC AGTGACCAAG 
ATCGCGACAA GCTATGGAGG ATCGGGTCAT GGAGCCGTCT CCCATCAGTA TGTCTCCAAG 
CCAGCAGTAG CCATCTCAGC AGCCCCCGCA ATCGCTAAGG TAGCGACATA CGCTTCTCCG 
GCAATATCAA CGTATGCCAC TGCTCCTGTG GTCTCAAAAG TGGCTACCTA CGCAGCTCCC 
TCCATAGCCA CTTACTCCTC CGCTCCGGCT CTAGCCAAGG TTTCCTATAG TCAGGCAGCG 
GATGTATCCC ACCAGTACAT TTCCAAACCT ATTGTAGCTG CCTATCCGGC AGCTGCTCCG 
GCAGTGGCCA CATACTCTTC CGCTCCTGCC ATCACCAAGC TCTCCACAAG CTATGAATCG 
TCAGGATCGG GAGCCGTCTC CCACCAGTAT GTGTCTAAAC CGGCGGTGGC TAAGGTCGCC 
GCATATGCAG CTCCAGCAAT CTCAACGTAT GCTACTGCTC CCGCCGTGTC CACTTACTCG 
TCAGCTCCAG TGGTAGCCAA AGTGGCCACT GGATACGGCG GAAGCGGATC GGGATACAGC 
TCCGGAGCAG TTTCACATCA ATATATCTCG AAGCCAGCAG TTGCCATTGC ATCAGCTCCA 
GCGATTGCCA AGGTAGCAAC ATATGCTGCT CCGGCCATAT CCGCCTATTC CTCGGCTCCC 
AGTCTCACCA AGATAGCCAC GGGCTATGGA GAATCGGCCC ATGGAGGAGC ACTCTCCCAC 
CAGTACATCT CTAAGCCGGC GATCGCAGTG GCTCCAGTGG CTCCTGTACT CTCCAAAACT 
TACTTGCCTG CCGCTCCAGC CGTCGCTAAA GTAGCTACAG GATATGGAAT AAGTGGTTCG 
GGAGCCGTGT CCCATCAGTA CGTATCCAAG CCAGCACTGA CACTTGCTGC GCCAGCGGTT 
GCCAAGGTGG CCACCTATGC TGCTCCGGCA ATATCCAGCT ACTCCACTGG AGCTGGCATA 
TCGAAAGGAT TGTCCTATGC AGCTCCTGTG GTTTCGACGG GCCACGGGTA CGGAGGAAGT 
GCCTCAGAAT ATAGTTCTGG AGCAGTCTCT CATCAGTATG TGTCCAAGCC AGCGGTTGCC 
ATTTCAGCTG CTCCTGCAAT TGCCAAAGTA GCAACGTATG TAGCTCCAGC TGTGGGTCAC 
ATTTTAGGTG GGCACGCCGT CGGCTCTGTT CCCGTTTTAT CCACTAAGCT GGCTACTGGC 
TACGGGGGCA GTGGATCGGG ATATAGCTCG GGAGCCGTTT CCCATCAATA TGTCTCCAAG 
CCAGCTGTGG CCATTTCAGC TGCTCCTGCT ATCGCCAAGG TGGCCGTTCC AACTGTCAGC 
CACATTTCCA CAGGTCCATC GACCCCGCTG GGAGTGGGAG TCGGGTACGG GTCTAGTGGA 
CATGGTGGAG TTTTGCTGGG TGCGCCGCTG ACGAAGTTGA CATCGGCTCC TGCTTATGCC 
CTGGGAGGAA AGCTGGCCAG CAGCACGGCC TACGGAATCC AAGCTGGGCA TGGATCTGGT 
CCCAGCGGTG GCTACTATGG TGCCATCTCC TTGGGTCATG CGAAGGTATC CCCGGCCCTT 
AGCTACCACG GTCTACTGTC CCATGGATCG GGTCTGGCAT CAGGCGCATC CTCTCTGGCC 
CATTTGGACT CCTCCCTGAG TGGATACTCA CACGGAGTGG GCGGAATCGG ACCGCTGGGA 
GCTGGATTCT ACCGCTATGC TCCAAGTGTG CCAGCGCTGA GCAGCCACGC CCCCGTGGCA 
GCCACCGCCT ACTTGAAGTC AGCACCCGTA ACTCAACATG CCGTCCTGAA GGTGGTGCCC 
GAGAAGCATC TGGAGCACTT CGATGCCCAT CCTCGATATG CCTTCGAGTA TGCCGTGAAT 
GATCCCCATA CGGGAGATAA CAAGCACCAG AAGGAGGAGC GAGATGGAGA CGTGGTCAAG 
GGAGAGTACT CCTTGGTTGA GCCCGATGGC AATGTGCGCA CGGTGAAGTA CTACGCCGAC 
TGGGAGACGG GCTTCCACGC CGAGGTCATC AACAGTCGCG ACCAGGGCAA AATTGTGGCC 
AAGCGTCAGA CGGAAACCAA GTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpr76Bd ( NM_001104175.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097645.1 Drosophila melanogaster cuticular protein 76Bd, transcript variant B (Cpr76Bd), mRNA.

Price & Availability↑

CloneID	OFa18972
Clone ID Related Accession (Same CDS sequence)	NM_001275124.1 , NM_001104175.3
Accession Version	NM_001104175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cuticular protein 76Bd, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104175.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTTC ATCTGGTGGC CCTCCTGGGC ATACTCAGCG TGGTACTTGC GGATCCGTCG 
CCCACCTTGG CTCCACCAAT CTTGCCCTGC GTACACGCAT CCACTGCTGG TTATGCCTAT 
CCTCCACCGG CGGAGGTAAA GTTCTCCATC TCGCCCGGAG TGACGAAGTA CAGCCAGAGC 
CCGGCGATAT CCACGTACTC GGAGAACGGA CAACTCCTGC ACACCTCGGT GGGAAGTTCC 
GGAGCCGCCT ATCAGTCTAC GGCCGGAATC GAGGGCTTGT CTCACGGTGG CATAACTCAG 
ATCAACAAAT ACATAGCTCC CGTGCAGAAG AGCCTGTTCT CTCCCTCAGC GGAGTACGGA 
GGAGCGGGAA TTACCTATGC GGACAAGTCA CCAGCGGCCA AGTACGCAAC CGTTGTGCCG 
TCGGGCATCG AAATCAAGAA CCTGGTGTCG CCGGCGGTTA CCAAGTTGGA CAACTCCTAC 
TTGCCACCAT CACCCGGAAT CACTAAAGTG GCGACATACA CCTCGCCCGG ATATAGTTAC 
AGTTCGGCAA GTCCGGGAAT ATCCAAAGTG GCGACTTACT CCTCCCCATC GCTTTCCAGC 
GTTCCCTACT ATGCGCCACC AGTGACTTCC AAGGTGGAGA CATACAGCTC ACCTGCGTAC 
ACGTACAGCA AGACCACACC CGGCTACTCC AAGGTGGAGA CCTACAGCTC TCCTGGTTAC 
AGCTATGGAC AGATCTCGCC GGGCATCTCC AGGATAGCCA CCTACTCGCC ATCGGTTTCG 
TATTCAGCGC CAACGATTGC CAAGGTGTCC ACCTACTCAG CGCCTTCAGT GAAACTGGCC 
ACCACTTCGT CACTGCTCTC CTCCCACGGA ACAGGATACT CCGCCAGCTA TGCTCCCTCA 
ATTACCAAGT ACAGCCAGGT ATCAGATGTA TCCCACCAGT ACATATCCAA GCCTATAGTG 
GCAGCCTATC CGGCGATCAC CAAAGTGGCG GCCAGCTACG GAGGCACTGC ATCCGGAGCG 
CTGTCCCACC AGTATGTCTC CCAACCAGCC ATTGCCAAGG TGTCCACTTA CGCTGCTCCC 
ACGGTTGCAA CTTACTCATC TGGGCCTGCC ATCTCGAAGC TCTCCACCAG CTATGGAGCT 
TCAGGATCGG GAGCCGTCTC CCACCAATAT GTCTCGAAGC CAGCTGTGGC TATTGCCGCT 
CCAGCAGTGG CCAAGGTGGC AACGTATGCA GCTCCTGCCA TTTCAAGCTA CTCCACTGGT 
CCTGCCATTT CGAAGGTAGC ATCCTACGCA GCTCCCACTG TCTCCACCTA CTCCTCGGGA 
TATGGTTATG GTTCGAGTGG GTCAGGGGCC GTATCGCACC AGTACGTGTC CAAGCCTGCG 
GTGGCCATTT CAGCCGCTCC AGCCATCGCC AAGGTGGCTA CCTATGCAGC TCCCGCCATT 
TCAACTTATG CGGCAGCTCC AGTGGTGACC AAAGTCGCCA CTGGATATGG AGGAAGTGGC 
TCGGGATACA GTTCGGGCGC TGTTTCCCAT CAATATGTCT CTAAGCCAGC GGTTGCTAAA 
GTGGCAACCT ATGCTGCTCC TGCCATTTCA ACATACTCAG CAGCTCCGGC AGTGACCAAG 
ATCGCGACAA GCTATGGAGG ATCGGGTCAT GGAGCCGTCT CCCATCAGTA TGTCTCCAAG 
CCAGCAGTAG CCATCTCAGC AGCCCCCGCA ATCGCTAAGG TAGCGACATA CGCTTCTCCG 
GCAATATCAA CGTATGCCAC TGCTCCTGTG GTCTCAAAAG TGGCTACCTA CGCAGCTCCC 
TCCATAGCCA CTTACTCCTC CGCTCCGGCT CTAGCCAAGG TTTCCTATAG TCAGGCAGCG 
GATGTATCCC ACCAGTACAT TTCCAAACCT ATTGTAGCTG CCTATCCGGC AGCTGCTCCG 
GCAGTGGCCA CATACTCTTC CGCTCCTGCC ATCACCAAGC TCTCCACAAG CTATGAATCG 
TCAGGATCGG GAGCCGTCTC CCACCAGTAT GTGTCTAAAC CGGCGGTGGC TAAGGTCGCC 
GCATATGCAG CTCCAGCAAT CTCAACGTAT GCTACTGCTC CCGCCGTGTC CACTTACTCG 
TCAGCTCCAG TGGTAGCCAA AGTGGCCACT GGATACGGCG GAAGCGGATC GGGATACAGC 
TCCGGAGCAG TTTCACATCA ATATATCTCG AAGCCAGCAG TTGCCATTGC ATCAGCTCCA 
GCGATTGCCA AGGTAGCAAC ATATGCTGCT CCGGCCATAT CCGCCTATTC CTCGGCTCCC 
AGTCTCACCA AGATAGCCAC GGGCTATGGA GAATCGGCCC ATGGAGGAGC ACTCTCCCAC 
CAGTACATCT CTAAGCCGGC GATCGCAGTG GCTCCAGTGG CTCCTGTACT CTCCAAAACT 
TACTTGCCTG CCGCTCCAGC CGTCGCTAAA GTAGCTACAG GATATGGAAT AAGTGGTTCG 
GGAGCCGTGT CCCATCAGTA CGTATCCAAG CCAGCACTGA CACTTGCTGC GCCAGCGGTT 
GCCAAGGTGG CCACCTATGC TGCTCCGGCA ATATCCAGCT ACTCCACTGG AGCTGGCATA 
TCGAAAGGAT TGTCCTATGC AGCTCCTGTG GTTTCGACGG GCCACGGGTA CGGAGGAAGT 
GCCTCAGAAT ATAGTTCTGG AGCAGTCTCT CATCAGTATG TGTCCAAGCC AGCGGTTGCC 
ATTTCAGCTG CTCCTGCAAT TGCCAAAGTA GCAACGTATG TAGCTCCAGC TGTGGGTCAC 
ATTTTAGGTG GGCACGCCGT CGGCTCTGTT CCCGTTTTAT CCACTAAGCT GGCTACTGGC 
TACGGGGGCA GTGGATCGGG ATATAGCTCG GGAGCCGTTT CCCATCAATA TGTCTCCAAG 
CCAGCTGTGG CCATTTCAGC TGCTCCTGCT ATCGCCAAGG TGGCCGTTCC AACTGTCAGC 
CACATTTCCA CAGGTCCATC GACCCCGCTG GGAGTGGGAG TCGGGTACGG GTCTAGTGGA 
CATGGTGGAG TTTTGCTGGG TGCGCCGCTG ACGAAGTTGA CATCGGCTCC TGCTTATGCC 
CTGGGAGGAA AGCTGGCCAG CAGCACGGCC TACGGAATCC AAGCTGGGCA TGGATCTGGT 
CCCAGCGGTG GCTACTATGG TGCCATCTCC TTGGGTCATG CGAAGGTATC CCCGGCCCTT 
AGCTACCACG GTCTACTGTC CCATGGATCG GGTCTGGCAT CAGGCGCATC CTCTCTGGCC 
CATTTGGACT CCTCCCTGAG TGGATACTCA CACGGAGTGG GCGGAATCGG ACCGCTGGGA 
GCTGGATTCT ACCGCTATGC TCCAAGTGTG CCAGCGCTGA GCAGCCACGC CCCCGTGGCA 
GCCACCGCCT ACTTGAAGTC AGCACCCGTA ACTCAACATG CCGTCCTGAA GGTGGTGCCC 
GAGAAGCATC TGGAGCACTT CGATGCCCAT CCTCGATATG CCTTCGAGTA TGCCGTGAAT 
GATCCCCATA CGGGAGATAA CAAGCACCAG AAGGAGGAGC GAGATGGAGA CGTGGTCAAG 
GGAGAGTACT CCTTGGTTGA GCCCGATGGC AATGTGCGCA CGGTGAAGTA CTACGCCGAC 
TGGGAGACGG GCTTCCACGC CGAGGTCATC AACAGTCGCG ACCAGGGCAA AATTGTGGCC 
AAGCGTCAGA CGGAAACCAA GTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097645.1
CDS	348..4034
Translation	MKLHLVALLGILSVVLADPSPTLAPPILPCVHASTAGYAYPPPAEVKFSISPGVTKYSQSPAISTYSENGQLLHTSVGSSGAAYQSTAGIEGLSHGGITQINKYIAPVQKSLFSPSAEYGGAGITYADKSPAAKYATVVPSGIEIKNLVSPAVTKLDNSYLPPSPGITKVATYTSPGYSYSSASPGISKVATYSSPSLSSVPYYAPPVTSKVETYSSPAYTYSKTTPGYSKVETYSSPGYSYGQISPGISRIATYSPSVSYSAPTIAKVSTYSAPSVKLATTSSLLSSHGTGYSASYAPSITKYSQVSDVSHQYISKPIVAAYPAITKVAASYGGTASGALSHQYVSQPAIAKVSTYAAPTVATYSSGPAISKLSTSYGASGSGAVSHQYVSKPAVAIAAPAVAKVATYAAPAISSYSTGPAISKVASYAAPTVSTYSSGYGYGSSGSGAVSHQYVSKPAVAISAAPAIAKVATYAAPAISTYAAAPVVTKVATGYGGSGSGYSSGAVSHQYVSKPAVAKVATYAAPAISTYSAAPAVTKIATSYGGSGHGAVSHQYVSKPAVAISAAPAIAKVATYASPAISTYATAPVVSKVATYAAPSIATYSSAPALAKVSYSQAADVSHQYISKPIVAAYPAAAPAVATYSSAPAITKLSTSYESSGSGAVSHQYVSKPAVAKVAAYAAPAISTYATAPAVSTYSSAPVVAKVATGYGGSGSGYSSGAVSHQYISKPAVAIASAPAIAKVATYAAPAISAYSSAPSLTKIATGYGESAHGGALSHQYISKPAIAVAPVAPVLSKTYLPAAPAVAKVATGYGISGSGAVSHQYVSKPALTLAAPAVAKVATYAAPAISSYSTGAGISKGLSYAAPVVSTGHGYGGSASEYSSGAVSHQYVSKPAVAISAAPAIAKVATYVAPAVGHILGGHAVGSVPVLSTKLATGYGGSGSGYSSGAVSHQYVSKPAVAISAAPAIAKVAVPTVSHISTGPSTPLGVGVGYGSSGHGGVLLGAPLTKLTSAPAYALGGKLASSTAYGIQAGHGSGPSGGYYGAISLGHAKVSPALSYHGLLSHGSGLASGASSLAHLDSSLSGYSHGVGGIGPLGAGFYRYAPSVPALSSHAPVAATAYLKSAPVTQHAVLKVVPEKHLEHFDAHPRYAFEYAVNDPHTGDNKHQKEERDGDVVKGEYSLVEPDGNVRTVKYYADWETGFHAEVINSRDQGKIVAKRQTETKS

Target ORF information:

RefSeq Version	NM_001104175.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cuticular protein 76Bd, transcript variant B (Cpr76Bd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104175.3

ORF Insert Sequence:

ATGAAGCTTC ATCTGGTGGC CCTCCTGGGC ATACTCAGCG TGGTACTTGC GGATCCGTCG 
CCCACCTTGG CTCCACCAAT CTTGCCCTGC GTACACGCAT CCACTGCTGG TTATGCCTAT 
CCTCCACCGG CGGAGGTAAA GTTCTCCATC TCGCCCGGAG TGACGAAGTA CAGCCAGAGC 
CCGGCGATAT CCACGTACTC GGAGAACGGA CAACTCCTGC ACACCTCGGT GGGAAGTTCC 
GGAGCCGCCT ATCAGTCTAC GGCCGGAATC GAGGGCTTGT CTCACGGTGG CATAACTCAG 
ATCAACAAAT ACATAGCTCC CGTGCAGAAG AGCCTGTTCT CTCCCTCAGC GGAGTACGGA 
GGAGCGGGAA TTACCTATGC GGACAAGTCA CCAGCGGCCA AGTACGCAAC CGTTGTGCCG 
TCGGGCATCG AAATCAAGAA CCTGGTGTCG CCGGCGGTTA CCAAGTTGGA CAACTCCTAC 
TTGCCACCAT CACCCGGAAT CACTAAAGTG GCGACATACA CCTCGCCCGG ATATAGTTAC 
AGTTCGGCAA GTCCGGGAAT ATCCAAAGTG GCGACTTACT CCTCCCCATC GCTTTCCAGC 
GTTCCCTACT ATGCGCCACC AGTGACTTCC AAGGTGGAGA CATACAGCTC ACCTGCGTAC 
ACGTACAGCA AGACCACACC CGGCTACTCC AAGGTGGAGA CCTACAGCTC TCCTGGTTAC 
AGCTATGGAC AGATCTCGCC GGGCATCTCC AGGATAGCCA CCTACTCGCC ATCGGTTTCG 
TATTCAGCGC CAACGATTGC CAAGGTGTCC ACCTACTCAG CGCCTTCAGT GAAACTGGCC 
ACCACTTCGT CACTGCTCTC CTCCCACGGA ACAGGATACT CCGCCAGCTA TGCTCCCTCA 
ATTACCAAGT ACAGCCAGGT ATCAGATGTA TCCCACCAGT ACATATCCAA GCCTATAGTG 
GCAGCCTATC CGGCGATCAC CAAAGTGGCG GCCAGCTACG GAGGCACTGC ATCCGGAGCG 
CTGTCCCACC AGTATGTCTC CCAACCAGCC ATTGCCAAGG TGTCCACTTA CGCTGCTCCC 
ACGGTTGCAA CTTACTCATC TGGGCCTGCC ATCTCGAAGC TCTCCACCAG CTATGGAGCT 
TCAGGATCGG GAGCCGTCTC CCACCAATAT GTCTCGAAGC CAGCTGTGGC TATTGCCGCT 
CCAGCAGTGG CCAAGGTGGC AACGTATGCA GCTCCTGCCA TTTCAAGCTA CTCCACTGGT 
CCTGCCATTT CGAAGGTAGC ATCCTACGCA GCTCCCACTG TCTCCACCTA CTCCTCGGGA 
TATGGTTATG GTTCGAGTGG GTCAGGGGCC GTATCGCACC AGTACGTGTC CAAGCCTGCG 
GTGGCCATTT CAGCCGCTCC AGCCATCGCC AAGGTGGCTA CCTATGCAGC TCCCGCCATT 
TCAACTTATG CGGCAGCTCC AGTGGTGACC AAAGTCGCCA CTGGATATGG AGGAAGTGGC 
TCGGGATACA GTTCGGGCGC TGTTTCCCAT CAATATGTCT CTAAGCCAGC GGTTGCTAAA 
GTGGCAACCT ATGCTGCTCC TGCCATTTCA ACATACTCAG CAGCTCCGGC AGTGACCAAG 
ATCGCGACAA GCTATGGAGG ATCGGGTCAT GGAGCCGTCT CCCATCAGTA TGTCTCCAAG 
CCAGCAGTAG CCATCTCAGC AGCCCCCGCA ATCGCTAAGG TAGCGACATA CGCTTCTCCG 
GCAATATCAA CGTATGCCAC TGCTCCTGTG GTCTCAAAAG TGGCTACCTA CGCAGCTCCC 
TCCATAGCCA CTTACTCCTC CGCTCCGGCT CTAGCCAAGG TTTCCTATAG TCAGGCAGCG 
GATGTATCCC ACCAGTACAT TTCCAAACCT ATTGTAGCTG CCTATCCGGC AGCTGCTCCG 
GCAGTGGCCA CATACTCTTC CGCTCCTGCC ATCACCAAGC TCTCCACAAG CTATGAATCG 
TCAGGATCGG GAGCCGTCTC CCACCAGTAT GTGTCTAAAC CGGCGGTGGC TAAGGTCGCC 
GCATATGCAG CTCCAGCAAT CTCAACGTAT GCTACTGCTC CCGCCGTGTC CACTTACTCG 
TCAGCTCCAG TGGTAGCCAA AGTGGCCACT GGATACGGCG GAAGCGGATC GGGATACAGC 
TCCGGAGCAG TTTCACATCA ATATATCTCG AAGCCAGCAG TTGCCATTGC ATCAGCTCCA 
GCGATTGCCA AGGTAGCAAC ATATGCTGCT CCGGCCATAT CCGCCTATTC CTCGGCTCCC 
AGTCTCACCA AGATAGCCAC GGGCTATGGA GAATCGGCCC ATGGAGGAGC ACTCTCCCAC 
CAGTACATCT CTAAGCCGGC GATCGCAGTG GCTCCAGTGG CTCCTGTACT CTCCAAAACT 
TACTTGCCTG CCGCTCCAGC CGTCGCTAAA GTAGCTACAG GATATGGAAT AAGTGGTTCG 
GGAGCCGTGT CCCATCAGTA CGTATCCAAG CCAGCACTGA CACTTGCTGC GCCAGCGGTT 
GCCAAGGTGG CCACCTATGC TGCTCCGGCA ATATCCAGCT ACTCCACTGG AGCTGGCATA 
TCGAAAGGAT TGTCCTATGC AGCTCCTGTG GTTTCGACGG GCCACGGGTA CGGAGGAAGT 
GCCTCAGAAT ATAGTTCTGG AGCAGTCTCT CATCAGTATG TGTCCAAGCC AGCGGTTGCC 
ATTTCAGCTG CTCCTGCAAT TGCCAAAGTA GCAACGTATG TAGCTCCAGC TGTGGGTCAC 
ATTTTAGGTG GGCACGCCGT CGGCTCTGTT CCCGTTTTAT CCACTAAGCT GGCTACTGGC 
TACGGGGGCA GTGGATCGGG ATATAGCTCG GGAGCCGTTT CCCATCAATA TGTCTCCAAG 
CCAGCTGTGG CCATTTCAGC TGCTCCTGCT ATCGCCAAGG TGGCCGTTCC AACTGTCAGC 
CACATTTCCA CAGGTCCATC GACCCCGCTG GGAGTGGGAG TCGGGTACGG GTCTAGTGGA 
CATGGTGGAG TTTTGCTGGG TGCGCCGCTG ACGAAGTTGA CATCGGCTCC TGCTTATGCC 
CTGGGAGGAA AGCTGGCCAG CAGCACGGCC TACGGAATCC AAGCTGGGCA TGGATCTGGT 
CCCAGCGGTG GCTACTATGG TGCCATCTCC TTGGGTCATG CGAAGGTATC CCCGGCCCTT 
AGCTACCACG GTCTACTGTC CCATGGATCG GGTCTGGCAT CAGGCGCATC CTCTCTGGCC 
CATTTGGACT CCTCCCTGAG TGGATACTCA CACGGAGTGG GCGGAATCGG ACCGCTGGGA 
GCTGGATTCT ACCGCTATGC TCCAAGTGTG CCAGCGCTGA GCAGCCACGC CCCCGTGGCA 
GCCACCGCCT ACTTGAAGTC AGCACCCGTA ACTCAACATG CCGTCCTGAA GGTGGTGCCC 
GAGAAGCATC TGGAGCACTT CGATGCCCAT CCTCGATATG CCTTCGAGTA TGCCGTGAAT 
GATCCCCATA CGGGAGATAA CAAGCACCAG AAGGAGGAGC GAGATGGAGA CGTGGTCAAG 
GGAGAGTACT CCTTGGTTGA GCCCGATGGC AATGTGCGCA CGGTGAAGTA CTACGCCGAC 
TGGGAGACGG GCTTCCACGC CGAGGTCATC AACAGTCGCG ACCAGGGCAA AATTGTGGCC 
AAGCGTCAGA CGGAAACCAA GTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cpr76Bd ( NM_001275123.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262052.1 Drosophila melanogaster cuticular protein 76Bd, transcript variant C (Cpr76Bd), mRNA.

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CloneID	OFa21085
Clone ID Related Accession (Same CDS sequence)	NM_001275123.1
Accession Version	NM_001275123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cuticular protein 76Bd, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTTC ATCTGGTGGC CCTCCTGGGC ATACTCAGCG TGGTACTTGC GGATCCGTCG 
CCCACCTTGG CTCCACCAAT CTTGCCCTGC GTACACGCAT CCACTGCTGG TTATGCCTAT 
CCTCCACCGG CGGAGGTAAA GTTCTCCATC TCGCCCGGAG TGACGAAGTA CAGCCAGAGC 
CCGGCGATAT CCACGTACTC GGAGAACGGA CAACTCCTGC ACACCTCGGT GGGAAGTTCC 
GGAGCCGCCT ATCAGTCTAC GGCCGGAATC GAGGGCTTGT CTCACGGTGG CATAACTCAG 
ATCAACAAAT ACATAGCTCC CGTGCAGAAG AGCCTGTTCT CTCCCTCAGC GGAGTACGGA 
GGAGCGGGAA TTACCTATGC GGACAAGTCA CCAGCGGCCA AGTACGCAAC CGTTGTGCCG 
TCGGGCATCG AAATCAAGAA CCTGGTGTCG CCGGCGGTTA CCAAGTTGGA CAACTCCTAC 
TTGCCACCAT CACCCGGAAT CACTAAAGTG GCGACATACA CCTCGCCCGG ATATAGTTAC 
AGTTCGGCAA GTCCGGGAAT ATCCAAAGTG GCGACTTACT CCTCCCCATC GCTTTCCAGC 
GTTCCCTACT ATGCGCCACC AGTGACTTCC AAGGTGGAGA CATACAGCTC ACCTGCGTAC 
ACGTACAGCA AGACCACACC CGGCTACTCC AAGGTGGAGA CCTACAGCTC TCCTGGTTAC 
AGCTATGGAC AGATCTCGCC GGGCATCTCC AGGATAGCCA CCTACTCGCC ATCGGTTTCG 
TATTCAGCGC CAACGATTGC CAAGGTGTCC ACCTACTCAG CGCCTTCAGT GAAACTGGCC 
ACCACTTCGT CACTGCTCTC CTCCCACGGA ACAGGATACT CCGCCAGCTA TGCTCCCTCA 
ATTACCAAGT ACAGCCAGGT ATCAGATGTA TCCCACCAGT ACATATCCAA GCCTATAGTG 
GCAGCCTATC CGGCGATCAC CAAAGTGGCG GCCAGCTACG GAGGCACTGC ATCCGGAGCG 
CTGTCCCACC AGTATGTCTC CCAACCAGCC ATTGCCAAGG TGTCCACTTA CGCTGCTCCC 
ACGGTTGCAA CTTACTCATC TGGGCCTGCC ATCTCGAAGC TCTCCACCAG CTATGGAGCT 
TCAGGATCGG GAGCCGTCTC CCACCAATAT GTCTCGAAGC CAGCTGTGGC TATTGCCGCT 
CCAGCAGTGG CCAAGGTGGC AACGTATGCA GCTCCTGCCA TTTCAAGCTA CTCCACTGGT 
CCTGCCATTT CGAAGGTAGC ATCCTACGCA GCTCCCACTG TCTCCACCTA CTCCTCGGGA 
TATGGTTATG GTTCGAGTGG GTCAGGGGCC GTATCGCACC AGTACGTGTC CAAGCCTGCG 
GTGGCCATTT CAGCCGCTCC AGCCATCGCC AAGGTGGCTA CCTATGCAGC TCCCGCCATT 
TCAACTTATG CGGCAGCTCC AGTGGTGACC AAAGTCGCCA CTGGATATGG AGGAAGTGGC 
TCGGGATACA GTTCGGGCGC TGTTTCCCAT CAATATGTCT CTAAGCCAGC GGTTGCTAAA 
GTGGCAACCT ATGCTGCTCC TGCCATTTCA ACATACTCAG CAGCTCCGGC AGTGACCAAG 
ATCGCGACAA GCTATGGAGG ATCGGGTCAT GGAGCCGTCT CCCATCAGTA TGTCTCCAAG 
CCAGCAGTAG CCATCTCAGC AGCCCCCGCA ATCGCTAAGG TAGCGACATA CGCTTCTCCG 
GCAATATCAA CGTATGCCAC TGCTCCTGTG GTCTCAAAAG TGGCTACCTA CGCAGCTCCC 
TCCATAGCCA CTTACTCCTC CGCTCCGGCT CTAGCCAAGG TTTCCTATAG TCAGGCAGCG 
GATGTATCCC ACCAGTACAT TTCCAAACCT ATTGTAGCTG CCTATCCGGC AGCTGCTCCG 
GCAGTGGCCA CATACTCTTC CGCTCCTGCC ATCACCAAGC TCTCCACAAG CTATGAATCG 
TCAGGATCGG GAGCCGTCTC CCACCAGTAT GTGTCTAAAC CGGCGGTGGC TAAGGTCGCC 
GCATATGCAG CTCCAGCAAT CTCAACGTAT GCTACTGCTC CCGCCGTGTC CACTTACTCG 
TCAGCTCCAG TGGTAGCCAA AGTGGCCACT GGATACGGCG GAAGCGGATC GGGATACAGC 
TCCGGAGCAG TTTCACATCA ATATATCTCG AAGCCAGCAG TTGCCATTGC ATCAGCTCCA 
GCGATTGCCA AGGTAGCAAC ATATGCTGCT CCGGCCATAT CCGCCTATTC CTCGGCTCCC 
AGTCTCACCA AGATAGCCAC GGGCTATGGA GAATCGGCCC ATGGAGGAGC ACTCTCCCAC 
CAGTACATCT CTAAGCCGGC GATCGCAGTG GCTCCAGTGG CTCCTGTACT CTCCAAAACT 
TACTTGCCTG CCGCTCCAGC CGTCGCTAAA GTAGCTACAG GATATGGAAT AAGTGGTTCG 
GGAGCCGTGT CCCATCAGTA CGTATCCAAG CCAGCACTGA CACTTGCTGC GCCAGCGGTT 
GCCAAGGTGG CCACCTATGC TGCTCCGGCA ATATCCAGCT ACTCCACTGG AGCTGGCATA 
TCGAAAGGAT TGTCCTATGC AGCTCCTGTG GTTTCGACGG GCCACGGGTA CGGAGGAAGT 
GCCTCAGAAT ATAGTTCTGG AGCAGTCTCT CATCAGTATG TGTCCAAGCC AGCGGTTGCC 
ATTTCAGCTG CTCCTGCAAT TGCCAAAGTA GCAACGTATG TAGCTCCAGC TGTGGGTCAC 
ATTTTAGGTG GGCACGCCGT CGGCTCTGTT CCCGTTTTAT CCACTAAGCT GGCTACTGGC 
TACGGGGGCA GTGGATCGGG ATATAGCTCG GGAGCCGTTT CCCATCAATA TGTCTCCAAG 
CCAGCTGTGG CCATTTCAGC TGCTCCTGCT ATCGCCAAGG TGGCCGTTCC AACTGTCAGC 
CACATTTCCA CAGGTCCATC GACCCCGCTG GGAGTGGGAG TCGGGTACGG GTCTAGTGGA 
CATGGTGGAG TTTTGCTGGG TGCGCCGCTG ACGAAGTTGA CATCGGCTCC TGCTTATGCC 
CTGGGAGGAA AGCTGGCCAG CAGCACGGCC TACGGAATCC AAGCTGGCAA TTTAGGGCAT 
GGATCTGGTC CCAGCGGTGG CTACTATGGT GCCATCTCCT TGGGTCATGC GAAGGTATCC 
CCGGCCCTTA GCTACCACGG TCTACTGTCC CATGGATCGG GTCTGGCATC AGGCGCATCC 
TCTCTGGCCC ATTTGGACTC CTCCCTGAGT GGATACTCAC ACGGAGTGGG CGGAATCGGA 
CCGCTGGGAG CTGGATTCTA CCGCTATGCT CCAAGTGTGC CAGCGCTGAG CAGCCACGCC 
CCCGTGGCAG CCACCGCCTA CTTGAAGTCA GCACCCGTAA CTCAACATGC CGTCCTGAAG 
GTGGTGCCCG AGAAGCATCT GGAGCACTTC GATGCCCATC CTCGATATGC CTTCGAGTAT 
GCCGTGAATG ATCCCCATAC GGGAGATAAC AAGCACCAGA AGGAGGAGCG AGATGGAGAC 
GTGGTCAAGG GAGAGTACTC CTTGGTTGAG CCCGATGGCA ATGTGCGCAC GGTGAAGTAC 
TACGCCGACT GGGAGACGGG CTTCCACGCC GAGGTCATCA ACAGTCGCGA CCAGGGCAAA 
ATTGTGGCCA AGCGTCAGAC GGAAACCAAG TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262052.1
CDS	348..4043
Translation	MKLHLVALLGILSVVLADPSPTLAPPILPCVHASTAGYAYPPPAEVKFSISPGVTKYSQSPAISTYSENGQLLHTSVGSSGAAYQSTAGIEGLSHGGITQINKYIAPVQKSLFSPSAEYGGAGITYADKSPAAKYATVVPSGIEIKNLVSPAVTKLDNSYLPPSPGITKVATYTSPGYSYSSASPGISKVATYSSPSLSSVPYYAPPVTSKVETYSSPAYTYSKTTPGYSKVETYSSPGYSYGQISPGISRIATYSPSVSYSAPTIAKVSTYSAPSVKLATTSSLLSSHGTGYSASYAPSITKYSQVSDVSHQYISKPIVAAYPAITKVAASYGGTASGALSHQYVSQPAIAKVSTYAAPTVATYSSGPAISKLSTSYGASGSGAVSHQYVSKPAVAIAAPAVAKVATYAAPAISSYSTGPAISKVASYAAPTVSTYSSGYGYGSSGSGAVSHQYVSKPAVAISAAPAIAKVATYAAPAISTYAAAPVVTKVATGYGGSGSGYSSGAVSHQYVSKPAVAKVATYAAPAISTYSAAPAVTKIATSYGGSGHGAVSHQYVSKPAVAISAAPAIAKVATYASPAISTYATAPVVSKVATYAAPSIATYSSAPALAKVSYSQAADVSHQYISKPIVAAYPAAAPAVATYSSAPAITKLSTSYESSGSGAVSHQYVSKPAVAKVAAYAAPAISTYATAPAVSTYSSAPVVAKVATGYGGSGSGYSSGAVSHQYISKPAVAIASAPAIAKVATYAAPAISAYSSAPSLTKIATGYGESAHGGALSHQYISKPAIAVAPVAPVLSKTYLPAAPAVAKVATGYGISGSGAVSHQYVSKPALTLAAPAVAKVATYAAPAISSYSTGAGISKGLSYAAPVVSTGHGYGGSASEYSSGAVSHQYVSKPAVAISAAPAIAKVATYVAPAVGHILGGHAVGSVPVLSTKLATGYGGSGSGYSSGAVSHQYVSKPAVAISAAPAIAKVAVPTVSHISTGPSTPLGVGVGYGSSGHGGVLLGAPLTKLTSAPAYALGGKLASSTAYGIQAGNLGHGSGPSGGYYGAISLGHAKVSPALSYHGLLSHGSGLASGASSLAHLDSSLSGYSHGVGGIGPLGAGFYRYAPSVPALSSHAPVAATAYLKSAPVTQHAVLKVVPEKHLEHFDAHPRYAFEYAVNDPHTGDNKHQKEERDGDVVKGEYSLVEPDGNVRTVKYYADWETGFHAEVINSRDQGKIVAKRQTETKS

Target ORF information:

RefSeq Version	NM_001275123.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cuticular protein 76Bd, transcript variant C (Cpr76Bd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275123.1

ORF Insert Sequence:

ATGAAGCTTC ATCTGGTGGC CCTCCTGGGC ATACTCAGCG TGGTACTTGC GGATCCGTCG 
CCCACCTTGG CTCCACCAAT CTTGCCCTGC GTACACGCAT CCACTGCTGG TTATGCCTAT 
CCTCCACCGG CGGAGGTAAA GTTCTCCATC TCGCCCGGAG TGACGAAGTA CAGCCAGAGC 
CCGGCGATAT CCACGTACTC GGAGAACGGA CAACTCCTGC ACACCTCGGT GGGAAGTTCC 
GGAGCCGCCT ATCAGTCTAC GGCCGGAATC GAGGGCTTGT CTCACGGTGG CATAACTCAG 
ATCAACAAAT ACATAGCTCC CGTGCAGAAG AGCCTGTTCT CTCCCTCAGC GGAGTACGGA 
GGAGCGGGAA TTACCTATGC GGACAAGTCA CCAGCGGCCA AGTACGCAAC CGTTGTGCCG 
TCGGGCATCG AAATCAAGAA CCTGGTGTCG CCGGCGGTTA CCAAGTTGGA CAACTCCTAC 
TTGCCACCAT CACCCGGAAT CACTAAAGTG GCGACATACA CCTCGCCCGG ATATAGTTAC 
AGTTCGGCAA GTCCGGGAAT ATCCAAAGTG GCGACTTACT CCTCCCCATC GCTTTCCAGC 
GTTCCCTACT ATGCGCCACC AGTGACTTCC AAGGTGGAGA CATACAGCTC ACCTGCGTAC 
ACGTACAGCA AGACCACACC CGGCTACTCC AAGGTGGAGA CCTACAGCTC TCCTGGTTAC 
AGCTATGGAC AGATCTCGCC GGGCATCTCC AGGATAGCCA CCTACTCGCC ATCGGTTTCG 
TATTCAGCGC CAACGATTGC CAAGGTGTCC ACCTACTCAG CGCCTTCAGT GAAACTGGCC 
ACCACTTCGT CACTGCTCTC CTCCCACGGA ACAGGATACT CCGCCAGCTA TGCTCCCTCA 
ATTACCAAGT ACAGCCAGGT ATCAGATGTA TCCCACCAGT ACATATCCAA GCCTATAGTG 
GCAGCCTATC CGGCGATCAC CAAAGTGGCG GCCAGCTACG GAGGCACTGC ATCCGGAGCG 
CTGTCCCACC AGTATGTCTC CCAACCAGCC ATTGCCAAGG TGTCCACTTA CGCTGCTCCC 
ACGGTTGCAA CTTACTCATC TGGGCCTGCC ATCTCGAAGC TCTCCACCAG CTATGGAGCT 
TCAGGATCGG GAGCCGTCTC CCACCAATAT GTCTCGAAGC CAGCTGTGGC TATTGCCGCT 
CCAGCAGTGG CCAAGGTGGC AACGTATGCA GCTCCTGCCA TTTCAAGCTA CTCCACTGGT 
CCTGCCATTT CGAAGGTAGC ATCCTACGCA GCTCCCACTG TCTCCACCTA CTCCTCGGGA 
TATGGTTATG GTTCGAGTGG GTCAGGGGCC GTATCGCACC AGTACGTGTC CAAGCCTGCG 
GTGGCCATTT CAGCCGCTCC AGCCATCGCC AAGGTGGCTA CCTATGCAGC TCCCGCCATT 
TCAACTTATG CGGCAGCTCC AGTGGTGACC AAAGTCGCCA CTGGATATGG AGGAAGTGGC 
TCGGGATACA GTTCGGGCGC TGTTTCCCAT CAATATGTCT CTAAGCCAGC GGTTGCTAAA 
GTGGCAACCT ATGCTGCTCC TGCCATTTCA ACATACTCAG CAGCTCCGGC AGTGACCAAG 
ATCGCGACAA GCTATGGAGG ATCGGGTCAT GGAGCCGTCT CCCATCAGTA TGTCTCCAAG 
CCAGCAGTAG CCATCTCAGC AGCCCCCGCA ATCGCTAAGG TAGCGACATA CGCTTCTCCG 
GCAATATCAA CGTATGCCAC TGCTCCTGTG GTCTCAAAAG TGGCTACCTA CGCAGCTCCC 
TCCATAGCCA CTTACTCCTC CGCTCCGGCT CTAGCCAAGG TTTCCTATAG TCAGGCAGCG 
GATGTATCCC ACCAGTACAT TTCCAAACCT ATTGTAGCTG CCTATCCGGC AGCTGCTCCG 
GCAGTGGCCA CATACTCTTC CGCTCCTGCC ATCACCAAGC TCTCCACAAG CTATGAATCG 
TCAGGATCGG GAGCCGTCTC CCACCAGTAT GTGTCTAAAC CGGCGGTGGC TAAGGTCGCC 
GCATATGCAG CTCCAGCAAT CTCAACGTAT GCTACTGCTC CCGCCGTGTC CACTTACTCG 
TCAGCTCCAG TGGTAGCCAA AGTGGCCACT GGATACGGCG GAAGCGGATC GGGATACAGC 
TCCGGAGCAG TTTCACATCA ATATATCTCG AAGCCAGCAG TTGCCATTGC ATCAGCTCCA 
GCGATTGCCA AGGTAGCAAC ATATGCTGCT CCGGCCATAT CCGCCTATTC CTCGGCTCCC 
AGTCTCACCA AGATAGCCAC GGGCTATGGA GAATCGGCCC ATGGAGGAGC ACTCTCCCAC 
CAGTACATCT CTAAGCCGGC GATCGCAGTG GCTCCAGTGG CTCCTGTACT CTCCAAAACT 
TACTTGCCTG CCGCTCCAGC CGTCGCTAAA GTAGCTACAG GATATGGAAT AAGTGGTTCG 
GGAGCCGTGT CCCATCAGTA CGTATCCAAG CCAGCACTGA CACTTGCTGC GCCAGCGGTT 
GCCAAGGTGG CCACCTATGC TGCTCCGGCA ATATCCAGCT ACTCCACTGG AGCTGGCATA 
TCGAAAGGAT TGTCCTATGC AGCTCCTGTG GTTTCGACGG GCCACGGGTA CGGAGGAAGT 
GCCTCAGAAT ATAGTTCTGG AGCAGTCTCT CATCAGTATG TGTCCAAGCC AGCGGTTGCC 
ATTTCAGCTG CTCCTGCAAT TGCCAAAGTA GCAACGTATG TAGCTCCAGC TGTGGGTCAC 
ATTTTAGGTG GGCACGCCGT CGGCTCTGTT CCCGTTTTAT CCACTAAGCT GGCTACTGGC 
TACGGGGGCA GTGGATCGGG ATATAGCTCG GGAGCCGTTT CCCATCAATA TGTCTCCAAG 
CCAGCTGTGG CCATTTCAGC TGCTCCTGCT ATCGCCAAGG TGGCCGTTCC AACTGTCAGC 
CACATTTCCA CAGGTCCATC GACCCCGCTG GGAGTGGGAG TCGGGTACGG GTCTAGTGGA 
CATGGTGGAG TTTTGCTGGG TGCGCCGCTG ACGAAGTTGA CATCGGCTCC TGCTTATGCC 
CTGGGAGGAA AGCTGGCCAG CAGCACGGCC TACGGAATCC AAGCTGGCAA TTTAGGGCAT 
GGATCTGGTC CCAGCGGTGG CTACTATGGT GCCATCTCCT TGGGTCATGC GAAGGTATCC 
CCGGCCCTTA GCTACCACGG TCTACTGTCC CATGGATCGG GTCTGGCATC AGGCGCATCC 
TCTCTGGCCC ATTTGGACTC CTCCCTGAGT GGATACTCAC ACGGAGTGGG CGGAATCGGA 
CCGCTGGGAG CTGGATTCTA CCGCTATGCT CCAAGTGTGC CAGCGCTGAG CAGCCACGCC 
CCCGTGGCAG CCACCGCCTA CTTGAAGTCA GCACCCGTAA CTCAACATGC CGTCCTGAAG 
GTGGTGCCCG AGAAGCATCT GGAGCACTTC GATGCCCATC CTCGATATGC CTTCGAGTAT 
GCCGTGAATG ATCCCCATAC GGGAGATAAC AAGCACCAGA AGGAGGAGCG AGATGGAGAC 
GTGGTCAAGG GAGAGTACTC CTTGGTTGAG CCCGATGGCA ATGTGCGCAC GGTGAAGTAC 
TACGCCGACT GGGAGACGGG CTTCCACGCC GAGGTCATCA ACAGTCGCGA CCAGGGCAAA 
ATTGTGGCCA AGCGTCAGAC GGAAACCAAG TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cpr76Bd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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