Mkp3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mkp3
Entrez Gene ID	40081
Full Name	Mitogen-activated protein kinase phosphatase 3
Synonyms	CG14080,CG14081,DMKP-3,Dmel\CG14080,Dmkp3,MKP3,dMKP3,mkp-3,mkp3
General protein information	Preferred Names Mitogen-activated protein kinase phosphatase 3 Names CG14080 gene product from transcript CG14080-RD CG14080-PA CG14080-PB CG14080-PC CG14080-PD ERK-inhibitory phosphatase MAP kinase phosphatase 3 MAPK phosphatase 3 MAPK phosphatase-3 MKP-3-like MKP-like Mkp3-PA Mkp3-PB Mkp3-PC Mkp3-PD drosophila MKP-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75F6-75F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_140830.2	NP_649087.1	Mitogen-activated protein kinase phosphatase 3, isoform B
NM_001275103.1	NP_001262032.1	Mitogen-activated protein kinase phosphatase 3, isoform D
NM_168785.1	NP_730385.1	Mitogen-activated protein kinase phosphatase 3, isoform A
NM_001275102.1	NP_001262031.1	Mitogen-activated protein kinase phosphatase 3, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DUSP7	XP_541858.4
Pan troglodytes (chimpanzee)	DUSP7	XP_526209.4
Mus musculus (house mouse)	Dusp7	NP_703189.3
Danio rerio (zebrafish)	dusp7	NP_956068.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012237	XP_320303.4
Xenopus tropicalis (tropical clawed frog)	dusp7	NP_001039042.1
Homo sapiens (human)	DUSP7	NP_001938.2
Bos taurus (cattle)	DUSP7	NP_001094764.2
	LOC698473	XP_001091142.2
Rattus norvegicus (Norway rat)	Dusp7	NP_001094017.1
Drosophila melanogaster (fruit fly)	Mkp3	NP_649087.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that Tay bridge antagonizes EGFR signalling in the Drosophila melanogaster wing disc and other tissues, and that the protein interacts with both Erk and Mkp3
Title: Tay bridge is a negative regulator of EGFR signalling and interacts with Erk and Mkp3 in the Drosophila melanogaster wing.

Study suggests that Mkp3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway.
Title: Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3.

MKP-3 is critically involved in cell differentiation, proliferation, and gene expression by suppressing the Ras/ERK pathway, specifically binding to ERK via the N-terminal ERK-binding domain of MKP-3.
Title: MKP-3 has essential roles as a negative regulator of the Ras/mitogen-activated protein kinase pathway during Drosophila development.

Drosophila ERK specifically regulates DMKP-3 in terms of its enzyme activity and protein stability esssential for regulation.
Title: Regulation of Drosophila MKP-3 by Drosophila ERK.

The following Mkp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mkp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18927	NM_168785.1 Latest version!	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa18928	NM_001275103.1 Latest version!	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18928	NM_140830.2 Latest version!	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21079	NM_001275102.1 Latest version!	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mkp3 ( NM_168785.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730385.1 Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18927
Clone ID Related Accession (Same CDS sequence)	NM_168785.1
Accession Version	NM_168785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mitogen-activated protein kinase phosphatase 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCTTA GGTCCCTGCG CATTTCCACA ACGCAATCCG ATTCCGCGTG CAGCAGTTCG 
GCGGAATCGT CGGATTGCGA GAGCTCCAGC CACCATCACC ACCACCACAG TCACCACAAC 
TACAACGAGG CGCCCGTGGA GATAATCCCT GGACTACTCT TCCTGGGAAA TGCCACACAC 
AGCTGCGACT CGGAAGCGTT GAAAAAGTAC AATATAAAGT ATGTTTTGAA TGTGACACCA 
GATTTGCCAA ATAAGTTCAA GGAGTCGGGC GACATCAAGT ATCTGCAGAT TCCGATCACG 
GATCACTACT CACAAGATTT GGCCATACAT TTCCCGGATG CCATACAGTT TATAGAGGAA 
GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC CACTGCCTGG CCGGAGTTTC GCGCTCGGTG 
ACCGTGACGC TCGCCTACTT GATGCACACG CGGGGCCTGA GTCTCAACGA CGCCTTCGCG 
ATGGTGCGGG ACCGGAAGCC GGATGTATCG CCCAACTTCC ACTTCATGCA GCAGCTGCTG 
TCCTTCGAGA GCCAACTGCG CCTGAGACCC GGCTCCAGGT TCTCGTGCAG CTGCATTGCG 
CCGGACTGCA ATTGCATGCA GACCACGGGC TTTATGGCCA CCCATCTGGC GAATGCTACC 
GGGGTCTCGC CAGACTCCGG CATCGAGTTC GATCGCTGGA CCCCGTCGGA CACGGGTCTT 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730385.1
CDS	148..873
Translation	MGLRSLRISTTQSDSACSSSAESSDCESSSHHHHHHSHHNYNEAPVEIIPGLLFLGNATHSCDSEALKKYNIKYVLNVTPDLPNKFKESGDIKYLQIPITDHYSQDLAIHFPDAIQFIEEARSASSVVLVHCLAGVSRSVTVTLAYLMHTRGLSLNDAFAMVRDRKPDVSPNFHFMQQLLSFESQLRLRPGSRFSCSCIAPDCNCMQTTGFMATHLANATGVSPDSGIEFDRWTPSDTGLK

Target ORF information:

RefSeq Version	NM_168785.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168785.1

ORF Insert Sequence:

ATGGGTCTTA GGTCCCTGCG CATTTCCACA ACGCAATCCG ATTCCGCGTG CAGCAGTTCG 
GCGGAATCGT CGGATTGCGA GAGCTCCAGC CACCATCACC ACCACCACAG TCACCACAAC 
TACAACGAGG CGCCCGTGGA GATAATCCCT GGACTACTCT TCCTGGGAAA TGCCACACAC 
AGCTGCGACT CGGAAGCGTT GAAAAAGTAC AATATAAAGT ATGTTTTGAA TGTGACACCA 
GATTTGCCAA ATAAGTTCAA GGAGTCGGGC GACATCAAGT ATCTGCAGAT TCCGATCACG 
GATCACTACT CACAAGATTT GGCCATACAT TTCCCGGATG CCATACAGTT TATAGAGGAA 
GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC CACTGCCTGG CCGGAGTTTC GCGCTCGGTG 
ACCGTGACGC TCGCCTACTT GATGCACACG CGGGGCCTGA GTCTCAACGA CGCCTTCGCG 
ATGGTGCGGG ACCGGAAGCC GGATGTATCG CCCAACTTCC ACTTCATGCA GCAGCTGCTG 
TCCTTCGAGA GCCAACTGCG CCTGAGACCC GGCTCCAGGT TCTCGTGCAG CTGCATTGCG 
CCGGACTGCA ATTGCATGCA GACCACGGGC TTTATGGCCA CCCATCTGGC GAATGCTACC 
GGGGTCTCGC CAGACTCCGG CATCGAGTTC GATCGCTGGA CCCCGTCGGA CACGGGTCTT 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkp3 ( NM_140830.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649087.1 Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18928
Clone ID Related Accession (Same CDS sequence)	NM_140830.2 , NM_001275103.1
Accession Version	NM_140830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mitogen-activated protein kinase phosphatase 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140830.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGAAA CGGAGCACGA GACCTGCAGC AAGGAGTGGC TGCAGTCCCA GTTGCGATCC 
CTGGACTCCA AGGACCTGAT CCTGTTGGAC TGCCGCGGCT CGCACGAGTA CAGTGAGTCG 
CACATCCGCG GAGCCGTCAA CCTATGCATA CCCAGCATCG TCCTCCGTCG TCTGGCGGTT 
GGCAAAATCG ATCTGGCCTC CACGATCAAG TCGCCCGAGC TGAAGCAACG CATCCAGTCG 
GGCTACAAGC TATGCTGGTT CATCCTCTAC AACGGCGAAG GCGTGCCCGG CCAGAATCAG 
GAGATCGCCG GAGCCGGATC CCTGGCCGTC GCCATGGACT CCATCATCAG CATCCTGCAC 
CGTCGCCTCA AGCAGGACGG CTGCCGCGTA GTTGCTTTAC AAGATGGCTT CAACAATTTT 
CGCCAGGCAT TTCCGGAATG GTGCGAGGAC GATAATCAGA CGCACAGCAA AGAGATCGAA 
TCTAGTCGCA ATGTTCAAAC CGATCAGTTA ATGGGTCTTA GGTCCCTGCG CATTTCCACA 
ACGCAATCCG ATTCCGCGTG CAGCAGTTCG GCGGAATCGT CGGATTGCGA GAGCTCCAGC 
CACCATCACC ACCACCACAG TCACCACAAC TACAACGAGG CGCCCGTGGA GATAATCCCT 
GGACTACTCT TCCTGGGAAA TGCCACACAC AGCTGCGACT CGGAAGCGTT GAAAAAGTAC 
AATATAAAGT ATGTTTTGAA TGTGACACCA GATTTGCCAA ATAAGTTCAA GGAGTCGGGC 
GACATCAAGT ATCTGCAGAT TCCGATCACG GATCACTACT CACAAGATTT GGCCATACAT 
TTCCCGGATG CCATACAGTT TATAGAGGAA GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC 
CACTGCCTGG CCGGAGTTTC GCGCTCGGTG ACCGTGACGC TCGCCTACTT GATGCACACG 
CGGGGCCTGA GTCTCAACGA CGCCTTCGCG ATGGTGCGGG ACCGGAAGCC GGATGTATCG 
CCCAACTTCC ACTTCATGCA GCAGCTGCTG TCCTTCGAGA GCCAACTGCG CCTGAGACCC 
GGCTCCAGGT TCTCGTGCAG CTGCATTGCG CCGGACTGCA ATTGCATGCA GACCACGGGC 
TTTATGGCCA CCCATCTGGC GAATGCTACC GGGGTCTCGC CAGACTCCGG CATCGAGTTC 
GATCGCTGGA CCCCGTCGGA CACGGGTCTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649087.1
CDS	704..1939
Translation	MPETEHETCSKEWLQSQLRSLDSKDLILLDCRGSHEYSESHIRGAVNLCIPSIVLRRLAVGKIDLASTIKSPELKQRIQSGYKLCWFILYNGEGVPGQNQEIAGAGSLAVAMDSIISILHRRLKQDGCRVVALQDGFNNFRQAFPEWCEDDNQTHSKEIESSRNVQTDQLMGLRSLRISTTQSDSACSSSAESSDCESSSHHHHHHSHHNYNEAPVEIIPGLLFLGNATHSCDSEALKKYNIKYVLNVTPDLPNKFKESGDIKYLQIPITDHYSQDLAIHFPDAIQFIEEARSASSVVLVHCLAGVSRSVTVTLAYLMHTRGLSLNDAFAMVRDRKPDVSPNFHFMQQLLSFESQLRLRPGSRFSCSCIAPDCNCMQTTGFMATHLANATGVSPDSGIEFDRWTPSDTGLK

Target ORF information:

RefSeq Version	NM_140830.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140830.2

ORF Insert Sequence:

ATGCCAGAAA CGGAGCACGA GACCTGCAGC AAGGAGTGGC TGCAGTCCCA GTTGCGATCC 
CTGGACTCCA AGGACCTGAT CCTGTTGGAC TGCCGCGGCT CGCACGAGTA CAGTGAGTCG 
CACATCCGCG GAGCCGTCAA CCTATGCATA CCCAGCATCG TCCTCCGTCG TCTGGCGGTT 
GGCAAAATCG ATCTGGCCTC CACGATCAAG TCGCCCGAGC TGAAGCAACG CATCCAGTCG 
GGCTACAAGC TATGCTGGTT CATCCTCTAC AACGGCGAAG GCGTGCCCGG CCAGAATCAG 
GAGATCGCCG GAGCCGGATC CCTGGCCGTC GCCATGGACT CCATCATCAG CATCCTGCAC 
CGTCGCCTCA AGCAGGACGG CTGCCGCGTA GTTGCTTTAC AAGATGGCTT CAACAATTTT 
CGCCAGGCAT TTCCGGAATG GTGCGAGGAC GATAATCAGA CGCACAGCAA AGAGATCGAA 
TCTAGTCGCA ATGTTCAAAC CGATCAGTTA ATGGGTCTTA GGTCCCTGCG CATTTCCACA 
ACGCAATCCG ATTCCGCGTG CAGCAGTTCG GCGGAATCGT CGGATTGCGA GAGCTCCAGC 
CACCATCACC ACCACCACAG TCACCACAAC TACAACGAGG CGCCCGTGGA GATAATCCCT 
GGACTACTCT TCCTGGGAAA TGCCACACAC AGCTGCGACT CGGAAGCGTT GAAAAAGTAC 
AATATAAAGT ATGTTTTGAA TGTGACACCA GATTTGCCAA ATAAGTTCAA GGAGTCGGGC 
GACATCAAGT ATCTGCAGAT TCCGATCACG GATCACTACT CACAAGATTT GGCCATACAT 
TTCCCGGATG CCATACAGTT TATAGAGGAA GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC 
CACTGCCTGG CCGGAGTTTC GCGCTCGGTG ACCGTGACGC TCGCCTACTT GATGCACACG 
CGGGGCCTGA GTCTCAACGA CGCCTTCGCG ATGGTGCGGG ACCGGAAGCC GGATGTATCG 
CCCAACTTCC ACTTCATGCA GCAGCTGCTG TCCTTCGAGA GCCAACTGCG CCTGAGACCC 
GGCTCCAGGT TCTCGTGCAG CTGCATTGCG CCGGACTGCA ATTGCATGCA GACCACGGGC 
TTTATGGCCA CCCATCTGGC GAATGCTACC GGGGTCTCGC CAGACTCCGG CATCGAGTTC 
GATCGCTGGA CCCCGTCGGA CACGGGTCTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkp3 ( NM_001275103.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262032.1 Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18928
Clone ID Related Accession (Same CDS sequence)	NM_140830.2 , NM_001275103.1
Accession Version	NM_001275103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mitogen-activated protein kinase phosphatase 3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGAAA CGGAGCACGA GACCTGCAGC AAGGAGTGGC TGCAGTCCCA GTTGCGATCC 
CTGGACTCCA AGGACCTGAT CCTGTTGGAC TGCCGCGGCT CGCACGAGTA CAGTGAGTCG 
CACATCCGCG GAGCCGTCAA CCTATGCATA CCCAGCATCG TCCTCCGTCG TCTGGCGGTT 
GGCAAAATCG ATCTGGCCTC CACGATCAAG TCGCCCGAGC TGAAGCAACG CATCCAGTCG 
GGCTACAAGC TATGCTGGTT CATCCTCTAC AACGGCGAAG GCGTGCCCGG CCAGAATCAG 
GAGATCGCCG GAGCCGGATC CCTGGCCGTC GCCATGGACT CCATCATCAG CATCCTGCAC 
CGTCGCCTCA AGCAGGACGG CTGCCGCGTA GTTGCTTTAC AAGATGGCTT CAACAATTTT 
CGCCAGGCAT TTCCGGAATG GTGCGAGGAC GATAATCAGA CGCACAGCAA AGAGATCGAA 
TCTAGTCGCA ATGTTCAAAC CGATCAGTTA ATGGGTCTTA GGTCCCTGCG CATTTCCACA 
ACGCAATCCG ATTCCGCGTG CAGCAGTTCG GCGGAATCGT CGGATTGCGA GAGCTCCAGC 
CACCATCACC ACCACCACAG TCACCACAAC TACAACGAGG CGCCCGTGGA GATAATCCCT 
GGACTACTCT TCCTGGGAAA TGCCACACAC AGCTGCGACT CGGAAGCGTT GAAAAAGTAC 
AATATAAAGT ATGTTTTGAA TGTGACACCA GATTTGCCAA ATAAGTTCAA GGAGTCGGGC 
GACATCAAGT ATCTGCAGAT TCCGATCACG GATCACTACT CACAAGATTT GGCCATACAT 
TTCCCGGATG CCATACAGTT TATAGAGGAA GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC 
CACTGCCTGG CCGGAGTTTC GCGCTCGGTG ACCGTGACGC TCGCCTACTT GATGCACACG 
CGGGGCCTGA GTCTCAACGA CGCCTTCGCG ATGGTGCGGG ACCGGAAGCC GGATGTATCG 
CCCAACTTCC ACTTCATGCA GCAGCTGCTG TCCTTCGAGA GCCAACTGCG CCTGAGACCC 
GGCTCCAGGT TCTCGTGCAG CTGCATTGCG CCGGACTGCA ATTGCATGCA GACCACGGGC 
TTTATGGCCA CCCATCTGGC GAATGCTACC GGGGTCTCGC CAGACTCCGG CATCGAGTTC 
GATCGCTGGA CCCCGTCGGA CACGGGTCTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262032.1
CDS	704..1939
Translation	MPETEHETCSKEWLQSQLRSLDSKDLILLDCRGSHEYSESHIRGAVNLCIPSIVLRRLAVGKIDLASTIKSPELKQRIQSGYKLCWFILYNGEGVPGQNQEIAGAGSLAVAMDSIISILHRRLKQDGCRVVALQDGFNNFRQAFPEWCEDDNQTHSKEIESSRNVQTDQLMGLRSLRISTTQSDSACSSSAESSDCESSSHHHHHHSHHNYNEAPVEIIPGLLFLGNATHSCDSEALKKYNIKYVLNVTPDLPNKFKESGDIKYLQIPITDHYSQDLAIHFPDAIQFIEEARSASSVVLVHCLAGVSRSVTVTLAYLMHTRGLSLNDAFAMVRDRKPDVSPNFHFMQQLLSFESQLRLRPGSRFSCSCIAPDCNCMQTTGFMATHLANATGVSPDSGIEFDRWTPSDTGLK

Target ORF information:

RefSeq Version	NM_001275103.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275103.1

ORF Insert Sequence:

ATGCCAGAAA CGGAGCACGA GACCTGCAGC AAGGAGTGGC TGCAGTCCCA GTTGCGATCC 
CTGGACTCCA AGGACCTGAT CCTGTTGGAC TGCCGCGGCT CGCACGAGTA CAGTGAGTCG 
CACATCCGCG GAGCCGTCAA CCTATGCATA CCCAGCATCG TCCTCCGTCG TCTGGCGGTT 
GGCAAAATCG ATCTGGCCTC CACGATCAAG TCGCCCGAGC TGAAGCAACG CATCCAGTCG 
GGCTACAAGC TATGCTGGTT CATCCTCTAC AACGGCGAAG GCGTGCCCGG CCAGAATCAG 
GAGATCGCCG GAGCCGGATC CCTGGCCGTC GCCATGGACT CCATCATCAG CATCCTGCAC 
CGTCGCCTCA AGCAGGACGG CTGCCGCGTA GTTGCTTTAC AAGATGGCTT CAACAATTTT 
CGCCAGGCAT TTCCGGAATG GTGCGAGGAC GATAATCAGA CGCACAGCAA AGAGATCGAA 
TCTAGTCGCA ATGTTCAAAC CGATCAGTTA ATGGGTCTTA GGTCCCTGCG CATTTCCACA 
ACGCAATCCG ATTCCGCGTG CAGCAGTTCG GCGGAATCGT CGGATTGCGA GAGCTCCAGC 
CACCATCACC ACCACCACAG TCACCACAAC TACAACGAGG CGCCCGTGGA GATAATCCCT 
GGACTACTCT TCCTGGGAAA TGCCACACAC AGCTGCGACT CGGAAGCGTT GAAAAAGTAC 
AATATAAAGT ATGTTTTGAA TGTGACACCA GATTTGCCAA ATAAGTTCAA GGAGTCGGGC 
GACATCAAGT ATCTGCAGAT TCCGATCACG GATCACTACT CACAAGATTT GGCCATACAT 
TTCCCGGATG CCATACAGTT TATAGAGGAA GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC 
CACTGCCTGG CCGGAGTTTC GCGCTCGGTG ACCGTGACGC TCGCCTACTT GATGCACACG 
CGGGGCCTGA GTCTCAACGA CGCCTTCGCG ATGGTGCGGG ACCGGAAGCC GGATGTATCG 
CCCAACTTCC ACTTCATGCA GCAGCTGCTG TCCTTCGAGA GCCAACTGCG CCTGAGACCC 
GGCTCCAGGT TCTCGTGCAG CTGCATTGCG CCGGACTGCA ATTGCATGCA GACCACGGGC 
TTTATGGCCA CCCATCTGGC GAATGCTACC GGGGTCTCGC CAGACTCCGG CATCGAGTTC 
GATCGCTGGA CCCCGTCGGA CACGGGTCTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkp3 ( NM_001275102.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262031.1 Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa21079
Clone ID Related Accession (Same CDS sequence)	NM_001275102.1
Accession Version	NM_001275102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mitogen-activated protein kinase phosphatase 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGAAA CGGAGCACGA GACCTGCAGC AAGGAGTGGC TGCAGTCCCA GTTGCGATCC 
CTGGACTCCA AGGACCTGAT CCTGTTGGAC TGCCGCGGCT CGCACGAGTA CAGTGAGTCG 
CACATCCGCG GAGCCGTCAA CCTATGCATA CCCAGCATCG TCCTCCGTCG TCTGGCGGTT 
GGCAAAATCG ATCTGGCCTC CACGATCAAG TCGCCCGAGC TGAAGCAACG CATCCAGTCG 
GGCTACAAGC TATGCTGGTT CATCCTCTAC AACGGCGAAG GCGTGCCCGG CCAGAATCAG 
GAGATCGCCG GAGCCGGATC CCTGGCCGTC GCCATGGACT CCATCATCAG CATCCTGCAC 
CGTCGCCTCA AGCAGGACGG CTGCCGCGTA GTTGCTTTAC AAGATGGCTT CAACAATTTT 
CGCCAGGCAT TTCCGGAATG GTGCGAGGAC GATAATCAGA CGCACAGCAA AGAGATCGAA 
TCTAGTCGCA ATGTTCAAAC CGATCAGTTA ATGGGTCTTA GGTCCCTGCG CATTTCCACA 
ACGCAATCCG ATTCCGCGTG CAGCAGTTCG GCGGAATCGT CGGATTGCGA GAGCTCCAGC 
CACCATCACC ACCACCACAG TCACCACAAC TACAACGAGG CGCCCGTGGA GATAATCCCT 
GGACTACTCT TCCTGGGAAA TGCCACACAC AGCTGCGACT CGGAAGCGTT GAAAAAGTAC 
AATATAAAGT ATGTTTTGAA TGTGACACCA GATTTGCCAA ATAAGTTCAA GGAGTCGGGC 
GACATCAAGT ATCTGCAGAT TCCGATCACG GATCACTACT CACAAGATTT GGCCATACAT 
TTCCCGGATG CCATACAGTT TATAGAGGAA GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC 
CACTGCCTGG CCGGAGTTTC GCGCTCGGTG ACCGTGACGC TCGCCTACTT GATGCACACG 
CGGGGCCTGA GTCTCAACGA CGCCTTCGCG ATGGTGCGGG ACCGGAAGCC GGATGTATCG 
CCCAACTTCC ACTTCATGCA GCAGCTGCTG TCCTTCGAGA GCCAACTGCG CCTGAGACCC 
GGCTCCAGGT TCTCGTGCAG CTGCATTGCG CCGGACTGCA ATTGCATGCA GACCACGGGC 
TTTATGGCCA CCCATCTGGC GAATGCTACC GGGGTCTCGC CAGACTCCGG CATCGAGTTC 
GATCGCTGGA CCCCGTCGGA CACGGGTCTT AAATGAGAAC AGAGTGGCGG TAAGAGTTTC 
GTGCTACCAC CAAGTCAGGA GGTCCCATTT GCAGCCGCCG CCACATCGCC GTTGCCCATG 
TGCCTGGCGG TTAACCCGGA CAACATGTCT CCGGCCAGCA CCACCAGCTC GTCCACATCG 
ACCACGACCA CCGCGGAGGC GGTTTCCGTG GTGGAAATGG TGCAGCATCG GGATCAGGAG 
ATGGCCGAAG AAGACATCAT AACCAGGGGA GAGTACGATG AGGATGCGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262031.1
CDS	704..2197
Translation	MPETEHETCSKEWLQSQLRSLDSKDLILLDCRGSHEYSESHIRGAVNLCIPSIVLRRLAVGKIDLASTIKSPELKQRIQSGYKLCWFILYNGEGVPGQNQEIAGAGSLAVAMDSIISILHRRLKQDGCRVVALQDGFNNFRQAFPEWCEDDNQTHSKEIESSRNVQTDQLMGLRSLRISTTQSDSACSSSAESSDCESSSHHHHHHSHHNYNEAPVEIIPGLLFLGNATHSCDSEALKKYNIKYVLNVTPDLPNKFKESGDIKYLQIPITDHYSQDLAIHFPDAIQFIEEARSASSVVLVHCLAGVSRSVTVTLAYLMHTRGLSLNDAFAMVRDRKPDVSPNFHFMQQLLSFESQLRLRPGSRFSCSCIAPDCNCMQTTGFMATHLANATGVSPDSGIEFDRWTPSDTGLK*EQSGGKSFVLPPSQEVPFAAAATSPLPMCLAVNPDNMSPASTTSSSTSTTTTAEAVSVVEMVQHRDQEMAEEDIITRGEYDEDAA

Target ORF information:

RefSeq Version	NM_001275102.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mitogen-activated protein kinase phosphatase 3 (Mkp3), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275102.1

ORF Insert Sequence:

ATGCCAGAAA CGGAGCACGA GACCTGCAGC AAGGAGTGGC TGCAGTCCCA GTTGCGATCC 
CTGGACTCCA AGGACCTGAT CCTGTTGGAC TGCCGCGGCT CGCACGAGTA CAGTGAGTCG 
CACATCCGCG GAGCCGTCAA CCTATGCATA CCCAGCATCG TCCTCCGTCG TCTGGCGGTT 
GGCAAAATCG ATCTGGCCTC CACGATCAAG TCGCCCGAGC TGAAGCAACG CATCCAGTCG 
GGCTACAAGC TATGCTGGTT CATCCTCTAC AACGGCGAAG GCGTGCCCGG CCAGAATCAG 
GAGATCGCCG GAGCCGGATC CCTGGCCGTC GCCATGGACT CCATCATCAG CATCCTGCAC 
CGTCGCCTCA AGCAGGACGG CTGCCGCGTA GTTGCTTTAC AAGATGGCTT CAACAATTTT 
CGCCAGGCAT TTCCGGAATG GTGCGAGGAC GATAATCAGA CGCACAGCAA AGAGATCGAA 
TCTAGTCGCA ATGTTCAAAC CGATCAGTTA ATGGGTCTTA GGTCCCTGCG CATTTCCACA 
ACGCAATCCG ATTCCGCGTG CAGCAGTTCG GCGGAATCGT CGGATTGCGA GAGCTCCAGC 
CACCATCACC ACCACCACAG TCACCACAAC TACAACGAGG CGCCCGTGGA GATAATCCCT 
GGACTACTCT TCCTGGGAAA TGCCACACAC AGCTGCGACT CGGAAGCGTT GAAAAAGTAC 
AATATAAAGT ATGTTTTGAA TGTGACACCA GATTTGCCAA ATAAGTTCAA GGAGTCGGGC 
GACATCAAGT ATCTGCAGAT TCCGATCACG GATCACTACT CACAAGATTT GGCCATACAT 
TTCCCGGATG CCATACAGTT TATAGAGGAA GCGCGGTCCG CAAGCTCGGT GGTGCTGGTC 
CACTGCCTGG CCGGAGTTTC GCGCTCGGTG ACCGTGACGC TCGCCTACTT GATGCACACG 
CGGGGCCTGA GTCTCAACGA CGCCTTCGCG ATGGTGCGGG ACCGGAAGCC GGATGTATCG 
CCCAACTTCC ACTTCATGCA GCAGCTGCTG TCCTTCGAGA GCCAACTGCG CCTGAGACCC 
GGCTCCAGGT TCTCGTGCAG CTGCATTGCG CCGGACTGCA ATTGCATGCA GACCACGGGC 
TTTATGGCCA CCCATCTGGC GAATGCTACC GGGGTCTCGC CAGACTCCGG CATCGAGTTC 
GATCGCTGGA CCCCGTCGGA CACGGGTCTT AAATGAGAAC AGAGTGGCGG TAAGAGTTTC 
GTGCTACCAC CAAGTCAGGA GGTCCCATTT GCAGCCGCCG CCACATCGCC GTTGCCCATG 
TGCCTGGCGG TTAACCCGGA CAACATGTCT CCGGCCAGCA CCACCAGCTC GTCCACATCG 
ACCACGACCA CCGCGGAGGC GGTTTCCGTG GTGGAAATGG TGCAGCATCG GGATCAGGAG 
ATGGCCGAAG AAGACATCAT AACCAGGGGA GAGTACGATG AGGATGCGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mkp3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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