TrpRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	TrpRS-m
Entrez Gene ID	39989
Full Name	Tryptophanyl-tRNA synthetase, mitochondrial
Synonyms	BEST:CK01295,CG7441,Dmel\CG7441,GH31
General protein information	Preferred Names Tryptophanyl-tRNA synthetase, mitochondrial Names CG7441 gene product from transcript CG7441-RA CG7441-PA CG7441-PB TrpRS-m-PA TrpRS-m-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75A4-75A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_140761.3	NP_649018.2	Tryptophanyl-tRNA synthetase, mitochondrial, isoform A
NM_001275072.1	NP_001262001.1	Tryptophanyl-tRNA synthetase, mitochondrial, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WARS2	XP_416536.4
Eremothecium gossypii	AGOS_ACR089C	NP_983491.1
Rattus norvegicus (Norway rat)	Wars2	NP_001162112.1
Kluyveromyces lactis	KLLA0E21825g	XP_454939.1
Arabidopsis thaliana (thale cress)	OVA4	NP_973533.1
Saccharomyces cerevisiae (baker's yeast)	MSW1	NP_010554.1
Oryza sativa (rice)	Os01g0743400	NP_001044218.1
Xenopus tropicalis (tropical clawed frog)	wars2	XP_004912356.1
Homo sapiens (human)	WARS2	NP_056651.1
	WARS2	XP_001113563.1
Bos taurus (cattle)	WARS2	NP_001029754.1
Mus musculus (house mouse)	Wars2	NP_081738.2
Drosophila melanogaster (fruit fly)	CG7441	NP_649018.2
Schizosaccharomyces pombe (fission yeast)	msw1	NP_594085.1
Magnaporthe oryzae (rice blast fungus)	MGG_14679	XP_003718589.1
Pan troglodytes (chimpanzee)	WARS2	XP_003308338.2
Canis lupus familiaris (dog)	WARS2	XP_005630737.1
Danio rerio (zebrafish)	wars2	NP_001013341.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011744	XP_320768.4
Caenorhabditis elegans (roundworm)	prx-10	NP_001021201.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TrpRS-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TrpRS-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18846	NM_140761.3 Latest version!	Drosophila melanogaster Tryptophanyl-tRNA synthetase, mitochondrial (TrpRS-m), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18846	NM_001275072.1 Latest version!	Drosophila melanogaster Tryptophanyl-tRNA synthetase, mitochondrial (TrpRS-m), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TrpRS-m ( NM_140761.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649018.2 Drosophila melanogaster Tryptophanyl-tRNA synthetase, mitochondrial (TrpRS-m), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18846
Clone ID Related Accession (Same CDS sequence)	NM_140761.3 , NM_001275072.1
Accession Version	NM_140761.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Tryptophanyl-tRNA synthetase, mitochondrial, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140761.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGAT TTGGCAAGCT CCATCGCAGT CTGTCTAAGG TGGCCAAAAA GGGACAGACA 
GCTGTGTATT CCCAGCGCCG TTGCATTCAG GCGAGGAGTT TCTTGCCGGG ATTGGCTAGT 
AGGACGGCTG GAACGCCCTC TGCGACGGCC CAGGTAACCG GTGGAAACAA GCAGCACGTA 
CACCCCCATG GTAATTCCGT TAACAGTGGC CACGAAGAAC ATAACACCCG CTGGCCGAGG 
AAAGTCTTTA GTGGCATCCA GCCGACGGGT TCCCTGCATC TGGGTAATTA TCTCGGCGCC 
GTGCGCAAAT GGGTGCAGCT GCAGAATGCT CGTGACGATG TCACCGTGTG TATTGTGGAT 
TTGCACTCTA TTACCATGCC CCACAATCCG CCGCTTCTGC GAGAGAACAT CTTCACCATG 
GCCGCCACGT TGTTGGCATG TGGCATCGAT CCCACCAAGA GCACCCTCTT TGTCCAGTCC 
GCCGTTGCCG AGCACGCCGA GTTCAATTGG ATCCTTAGCT CCTTGACCAC CATGCCGCGA 
TTGGCTCAAC TGCCGCAGTT CCGAGAGAAG TCCCGTCTTC TAAAGGATGT CCCTCTGGGA 
TTGTACGTTT ACCCGGTTCT CCAAGCAGCC GACATTATGC TTTACAAGTC CACTCATGTT 
CCTGTGGGCG CTGACCAGAT TCAGCACATT CAGCTGGCCC AGCACTTGGC CAGAATTTAT 
AACGGTCGCT ATGGCGAGAC TTTCCCTGTT TGCACCGCCA TCATTGAGGA TGGTGATGCC 
TCCCGTGTGC TGTCCCTGCG AGATCCCTCC AAAAAGATGT CTAAATCGGA AGCGAATCCC 
AAGGCCACCA TCAACTTGTG TGACTCGCCC GACTTGATTA CGCAGAAGAT AAAGAAAGCC 
GTCACAGACT TTACATCGGA CATTACTTAT AATCCTGGCA AGCGTGCTGG CGTCAGCAAT 
CTGGTCAACA TCCATGCCCA GGTCACGGGA CAGAGCATTA AGACAGTGGT GAACGAGGCC 
GCCACTTTGG ACACGGCAAA GTATAAGGAC CGTGTGGCGG AGGCTGTGGT CGAGCACTTG 
CGTCCGATCC GAGAACAGAT ACACCATCAC ATGACTAAGC GCAACGAGAT GATCTACTTG 
CTGGAAGTGG GTGCAGAAAA GGCACGTCAG CAGGCACGCC AAACGTTGAA CGATGTTAAA 
CAGCGCCTAG GACTAGGAAC CAGTGCCAAC ATTCCGGCTG CGGTGCATGT GGCTCCACTT 
CTGCCCGCAC CCGATATTTC CAAAACCTCA GCAAGCAGGC GAATGTCCAA GGACTTCGAC 
GGGAATGTCC ACGAGCGTCA CGAACGGATG TACGGATTCG GAGATCAGCC AAGCGGAGGA 
GCTGGTCAGT CAGCCACCGG CGAGGTGGCG GATATGCCTC AGGCCGTTGT ACCGCGGGTG 
ATCCGTCGGG CGCCCATCGT TCCCACTCAG TCTATGCGCG TAGAGAAACA ACTAGGTAGC 
ACTCGAACTC GGCACGTATT CCAGATGGAC ACCTGCAATG CTCCGCATAT AGGGTTCAAA 
CCGCCGCCAG GATTTTCGGC CAGCATGGTC ACCGGTGCTC TAGCCAAGAA CAAGCGCGCT 
ACAGTTAAAC CCGTTACTGG CAACCTGGGC TTCGGCCAGC AATCGCAGGG TGGCTCTTAC 
AAGCACAGCC AGAACGTGAA TCCTAGTGCG GTATCCACCA TTGCACGTGA AGTAAACGCT 
GCTAAGAAGC AGGAGTTTAC CTGCAACCCC GCTTTGTACG GCTCTGCCTT AGAATCGCTT 
TCCAATTCAA GTCGCTCGAG CGAAAAGACT AACTCTACGG CAGGAGACAG TGAGTTCATA 
ATTGTCGCGA GCGAGGAGGA AGAGCAGGCT GCTTCCTCTG AGGAGCAACG CGAAGAAGCT 
GAAGAGGAGG AGTCCGGGGA ACGTGAAAGG GAGAAAGGCG AGCGCAACGC CACTGAAGCT 
ACCAAAGTCG AGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649018.2
CDS	154..2151
Translation	MFRFGKLHRSLSKVAKKGQTAVYSQRRCIQARSFLPGLASRTAGTPSATAQVTGGNKQHVHPHGNSVNSGHEEHNTRWPRKVFSGIQPTGSLHLGNYLGAVRKWVQLQNARDDVTVCIVDLHSITMPHNPPLLRENIFTMAATLLACGIDPTKSTLFVQSAVAEHAEFNWILSSLTTMPRLAQLPQFREKSRLLKDVPLGLYVYPVLQAADIMLYKSTHVPVGADQIQHIQLAQHLARIYNGRYGETFPVCTAIIEDGDASRVLSLRDPSKKMSKSEANPKATINLCDSPDLITQKIKKAVTDFTSDITYNPGKRAGVSNLVNIHAQVTGQSIKTVVNEAATLDTAKYKDRVAEAVVEHLRPIREQIHHHMTKRNEMIYLLEVGAEKARQQARQTLNDVKQRLGLGTSANIPAAVHVAPLLPAPDISKTSASRRMSKDFDGNVHERHERMYGFGDQPSGGAGQSATGEVADMPQAVVPRVIRRAPIVPTQSMRVEKQLGSTRTRHVFQMDTCNAPHIGFKPPPGFSASMVTGALAKNKRATVKPVTGNLGFGQQSQGGSYKHSQNVNPSAVSTIAREVNAAKKQEFTCNPALYGSALESLSNSSRSSEKTNSTAGDSEFIIVASEEEEQAASSEEQREEAEEEESGEREREKGERNATEATKVEI

Target ORF information:

RefSeq Version	NM_140761.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Tryptophanyl-tRNA synthetase, mitochondrial (TrpRS-m), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140761.3

ORF Insert Sequence:

ATGTTCCGAT TTGGCAAGCT CCATCGCAGT CTGTCTAAGG TGGCCAAAAA GGGACAGACA 
GCTGTGTATT CCCAGCGCCG TTGCATTCAG GCGAGGAGTT TCTTGCCGGG ATTGGCTAGT 
AGGACGGCTG GAACGCCCTC TGCGACGGCC CAGGTAACCG GTGGAAACAA GCAGCACGTA 
CACCCCCATG GTAATTCCGT TAACAGTGGC CACGAAGAAC ATAACACCCG CTGGCCGAGG 
AAAGTCTTTA GTGGCATCCA GCCGACGGGT TCCCTGCATC TGGGTAATTA TCTCGGCGCC 
GTGCGCAAAT GGGTGCAGCT GCAGAATGCT CGTGACGATG TCACCGTGTG TATTGTGGAT 
TTGCACTCTA TTACCATGCC CCACAATCCG CCGCTTCTGC GAGAGAACAT CTTCACCATG 
GCCGCCACGT TGTTGGCATG TGGCATCGAT CCCACCAAGA GCACCCTCTT TGTCCAGTCC 
GCCGTTGCCG AGCACGCCGA GTTCAATTGG ATCCTTAGCT CCTTGACCAC CATGCCGCGA 
TTGGCTCAAC TGCCGCAGTT CCGAGAGAAG TCCCGTCTTC TAAAGGATGT CCCTCTGGGA 
TTGTACGTTT ACCCGGTTCT CCAAGCAGCC GACATTATGC TTTACAAGTC CACTCATGTT 
CCTGTGGGCG CTGACCAGAT TCAGCACATT CAGCTGGCCC AGCACTTGGC CAGAATTTAT 
AACGGTCGCT ATGGCGAGAC TTTCCCTGTT TGCACCGCCA TCATTGAGGA TGGTGATGCC 
TCCCGTGTGC TGTCCCTGCG AGATCCCTCC AAAAAGATGT CTAAATCGGA AGCGAATCCC 
AAGGCCACCA TCAACTTGTG TGACTCGCCC GACTTGATTA CGCAGAAGAT AAAGAAAGCC 
GTCACAGACT TTACATCGGA CATTACTTAT AATCCTGGCA AGCGTGCTGG CGTCAGCAAT 
CTGGTCAACA TCCATGCCCA GGTCACGGGA CAGAGCATTA AGACAGTGGT GAACGAGGCC 
GCCACTTTGG ACACGGCAAA GTATAAGGAC CGTGTGGCGG AGGCTGTGGT CGAGCACTTG 
CGTCCGATCC GAGAACAGAT ACACCATCAC ATGACTAAGC GCAACGAGAT GATCTACTTG 
CTGGAAGTGG GTGCAGAAAA GGCACGTCAG CAGGCACGCC AAACGTTGAA CGATGTTAAA 
CAGCGCCTAG GACTAGGAAC CAGTGCCAAC ATTCCGGCTG CGGTGCATGT GGCTCCACTT 
CTGCCCGCAC CCGATATTTC CAAAACCTCA GCAAGCAGGC GAATGTCCAA GGACTTCGAC 
GGGAATGTCC ACGAGCGTCA CGAACGGATG TACGGATTCG GAGATCAGCC AAGCGGAGGA 
GCTGGTCAGT CAGCCACCGG CGAGGTGGCG GATATGCCTC AGGCCGTTGT ACCGCGGGTG 
ATCCGTCGGG CGCCCATCGT TCCCACTCAG TCTATGCGCG TAGAGAAACA ACTAGGTAGC 
ACTCGAACTC GGCACGTATT CCAGATGGAC ACCTGCAATG CTCCGCATAT AGGGTTCAAA 
CCGCCGCCAG GATTTTCGGC CAGCATGGTC ACCGGTGCTC TAGCCAAGAA CAAGCGCGCT 
ACAGTTAAAC CCGTTACTGG CAACCTGGGC TTCGGCCAGC AATCGCAGGG TGGCTCTTAC 
AAGCACAGCC AGAACGTGAA TCCTAGTGCG GTATCCACCA TTGCACGTGA AGTAAACGCT 
GCTAAGAAGC AGGAGTTTAC CTGCAACCCC GCTTTGTACG GCTCTGCCTT AGAATCGCTT 
TCCAATTCAA GTCGCTCGAG CGAAAAGACT AACTCTACGG CAGGAGACAG TGAGTTCATA 
ATTGTCGCGA GCGAGGAGGA AGAGCAGGCT GCTTCCTCTG AGGAGCAACG CGAAGAAGCT 
GAAGAGGAGG AGTCCGGGGA ACGTGAAAGG GAGAAAGGCG AGCGCAACGC CACTGAAGCT 
ACCAAAGTCG AGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TrpRS-m ( NM_001275072.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262001.1 Drosophila melanogaster Tryptophanyl-tRNA synthetase, mitochondrial (TrpRS-m), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18846
Clone ID Related Accession (Same CDS sequence)	NM_140761.3 , NM_001275072.1
Accession Version	NM_001275072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Tryptophanyl-tRNA synthetase, mitochondrial, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGAT TTGGCAAGCT CCATCGCAGT CTGTCTAAGG TGGCCAAAAA GGGACAGACA 
GCTGTGTATT CCCAGCGCCG TTGCATTCAG GCGAGGAGTT TCTTGCCGGG ATTGGCTAGT 
AGGACGGCTG GAACGCCCTC TGCGACGGCC CAGGTAACCG GTGGAAACAA GCAGCACGTA 
CACCCCCATG GTAATTCCGT TAACAGTGGC CACGAAGAAC ATAACACCCG CTGGCCGAGG 
AAAGTCTTTA GTGGCATCCA GCCGACGGGT TCCCTGCATC TGGGTAATTA TCTCGGCGCC 
GTGCGCAAAT GGGTGCAGCT GCAGAATGCT CGTGACGATG TCACCGTGTG TATTGTGGAT 
TTGCACTCTA TTACCATGCC CCACAATCCG CCGCTTCTGC GAGAGAACAT CTTCACCATG 
GCCGCCACGT TGTTGGCATG TGGCATCGAT CCCACCAAGA GCACCCTCTT TGTCCAGTCC 
GCCGTTGCCG AGCACGCCGA GTTCAATTGG ATCCTTAGCT CCTTGACCAC CATGCCGCGA 
TTGGCTCAAC TGCCGCAGTT CCGAGAGAAG TCCCGTCTTC TAAAGGATGT CCCTCTGGGA 
TTGTACGTTT ACCCGGTTCT CCAAGCAGCC GACATTATGC TTTACAAGTC CACTCATGTT 
CCTGTGGGCG CTGACCAGAT TCAGCACATT CAGCTGGCCC AGCACTTGGC CAGAATTTAT 
AACGGTCGCT ATGGCGAGAC TTTCCCTGTT TGCACCGCCA TCATTGAGGA TGGTGATGCC 
TCCCGTGTGC TGTCCCTGCG AGATCCCTCC AAAAAGATGT CTAAATCGGA AGCGAATCCC 
AAGGCCACCA TCAACTTGTG TGACTCGCCC GACTTGATTA CGCAGAAGAT AAAGAAAGCC 
GTCACAGACT TTACATCGGA CATTACTTAT AATCCTGGCA AGCGTGCTGG CGTCAGCAAT 
CTGGTCAACA TCCATGCCCA GGTCACGGGA CAGAGCATTA AGACAGTGGT GAACGAGGCC 
GCCACTTTGG ACACGGCAAA GTATAAGGAC CGTGTGGCGG AGGCTGTGGT CGAGCACTTG 
CGTCCGATCC GAGAACAGAT ACACCATCAC ATGACTAAGC GCAACGAGAT GATCTACTTG 
CTGGAAGTGG GTGCAGAAAA GGCACGTCAG CAGGCACGCC AAACGTTGAA CGATGTTAAA 
CAGCGCCTAG GACTAGGAAC CAGTGCCAAC ATTCCGGCTG CGGTGCATGT GGCTCCACTT 
CTGCCCGCAC CCGATATTTC CAAAACCTCA GCAAGCAGGC GAATGTCCAA GGACTTCGAC 
GGGAATGTCC ACGAGCGTCA CGAACGGATG TACGGATTCG GAGATCAGCC AAGCGGAGGA 
GCTGGTCAGT CAGCCACCGG CGAGGTGGCG GATATGCCTC AGGCCGTTGT ACCGCGGGTG 
ATCCGTCGGG CGCCCATCGT TCCCACTCAG TCTATGCGCG TAGAGAAACA ACTAGGTAGC 
ACTCGAACTC GGCACGTATT CCAGATGGAC ACCTGCAATG CTCCGCATAT AGGGTTCAAA 
CCGCCGCCAG GATTTTCGGC CAGCATGGTC ACCGGTGCTC TAGCCAAGAA CAAGCGCGCT 
ACAGTTAAAC CCGTTACTGG CAACCTGGGC TTCGGCCAGC AATCGCAGGG TGGCTCTTAC 
AAGCACAGCC AGAACGTGAA TCCTAGTGCG GTATCCACCA TTGCACGTGA AGTAAACGCT 
GCTAAGAAGC AGGAGTTTAC CTGCAACCCC GCTTTGTACG GCTCTGCCTT AGAATCGCTT 
TCCAATTCAA GTCGCTCGAG CGAAAAGACT AACTCTACGG CAGGAGACAG TGAGTTCATA 
ATTGTCGCGA GCGAGGAGGA AGAGCAGGCT GCTTCCTCTG AGGAGCAACG CGAAGAAGCT 
GAAGAGGAGG AGTCCGGGGA ACGTGAAAGG GAGAAAGGCG AGCGCAACGC CACTGAAGCT 
ACCAAAGTCG AGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262001.1
CDS	154..2151
Translation	MFRFGKLHRSLSKVAKKGQTAVYSQRRCIQARSFLPGLASRTAGTPSATAQVTGGNKQHVHPHGNSVNSGHEEHNTRWPRKVFSGIQPTGSLHLGNYLGAVRKWVQLQNARDDVTVCIVDLHSITMPHNPPLLRENIFTMAATLLACGIDPTKSTLFVQSAVAEHAEFNWILSSLTTMPRLAQLPQFREKSRLLKDVPLGLYVYPVLQAADIMLYKSTHVPVGADQIQHIQLAQHLARIYNGRYGETFPVCTAIIEDGDASRVLSLRDPSKKMSKSEANPKATINLCDSPDLITQKIKKAVTDFTSDITYNPGKRAGVSNLVNIHAQVTGQSIKTVVNEAATLDTAKYKDRVAEAVVEHLRPIREQIHHHMTKRNEMIYLLEVGAEKARQQARQTLNDVKQRLGLGTSANIPAAVHVAPLLPAPDISKTSASRRMSKDFDGNVHERHERMYGFGDQPSGGAGQSATGEVADMPQAVVPRVIRRAPIVPTQSMRVEKQLGSTRTRHVFQMDTCNAPHIGFKPPPGFSASMVTGALAKNKRATVKPVTGNLGFGQQSQGGSYKHSQNVNPSAVSTIAREVNAAKKQEFTCNPALYGSALESLSNSSRSSEKTNSTAGDSEFIIVASEEEEQAASSEEQREEAEEEESGEREREKGERNATEATKVEI

Target ORF information:

RefSeq Version	NM_001275072.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Tryptophanyl-tRNA synthetase, mitochondrial (TrpRS-m), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275072.1

ORF Insert Sequence:

ATGTTCCGAT TTGGCAAGCT CCATCGCAGT CTGTCTAAGG TGGCCAAAAA GGGACAGACA 
GCTGTGTATT CCCAGCGCCG TTGCATTCAG GCGAGGAGTT TCTTGCCGGG ATTGGCTAGT 
AGGACGGCTG GAACGCCCTC TGCGACGGCC CAGGTAACCG GTGGAAACAA GCAGCACGTA 
CACCCCCATG GTAATTCCGT TAACAGTGGC CACGAAGAAC ATAACACCCG CTGGCCGAGG 
AAAGTCTTTA GTGGCATCCA GCCGACGGGT TCCCTGCATC TGGGTAATTA TCTCGGCGCC 
GTGCGCAAAT GGGTGCAGCT GCAGAATGCT CGTGACGATG TCACCGTGTG TATTGTGGAT 
TTGCACTCTA TTACCATGCC CCACAATCCG CCGCTTCTGC GAGAGAACAT CTTCACCATG 
GCCGCCACGT TGTTGGCATG TGGCATCGAT CCCACCAAGA GCACCCTCTT TGTCCAGTCC 
GCCGTTGCCG AGCACGCCGA GTTCAATTGG ATCCTTAGCT CCTTGACCAC CATGCCGCGA 
TTGGCTCAAC TGCCGCAGTT CCGAGAGAAG TCCCGTCTTC TAAAGGATGT CCCTCTGGGA 
TTGTACGTTT ACCCGGTTCT CCAAGCAGCC GACATTATGC TTTACAAGTC CACTCATGTT 
CCTGTGGGCG CTGACCAGAT TCAGCACATT CAGCTGGCCC AGCACTTGGC CAGAATTTAT 
AACGGTCGCT ATGGCGAGAC TTTCCCTGTT TGCACCGCCA TCATTGAGGA TGGTGATGCC 
TCCCGTGTGC TGTCCCTGCG AGATCCCTCC AAAAAGATGT CTAAATCGGA AGCGAATCCC 
AAGGCCACCA TCAACTTGTG TGACTCGCCC GACTTGATTA CGCAGAAGAT AAAGAAAGCC 
GTCACAGACT TTACATCGGA CATTACTTAT AATCCTGGCA AGCGTGCTGG CGTCAGCAAT 
CTGGTCAACA TCCATGCCCA GGTCACGGGA CAGAGCATTA AGACAGTGGT GAACGAGGCC 
GCCACTTTGG ACACGGCAAA GTATAAGGAC CGTGTGGCGG AGGCTGTGGT CGAGCACTTG 
CGTCCGATCC GAGAACAGAT ACACCATCAC ATGACTAAGC GCAACGAGAT GATCTACTTG 
CTGGAAGTGG GTGCAGAAAA GGCACGTCAG CAGGCACGCC AAACGTTGAA CGATGTTAAA 
CAGCGCCTAG GACTAGGAAC CAGTGCCAAC ATTCCGGCTG CGGTGCATGT GGCTCCACTT 
CTGCCCGCAC CCGATATTTC CAAAACCTCA GCAAGCAGGC GAATGTCCAA GGACTTCGAC 
GGGAATGTCC ACGAGCGTCA CGAACGGATG TACGGATTCG GAGATCAGCC AAGCGGAGGA 
GCTGGTCAGT CAGCCACCGG CGAGGTGGCG GATATGCCTC AGGCCGTTGT ACCGCGGGTG 
ATCCGTCGGG CGCCCATCGT TCCCACTCAG TCTATGCGCG TAGAGAAACA ACTAGGTAGC 
ACTCGAACTC GGCACGTATT CCAGATGGAC ACCTGCAATG CTCCGCATAT AGGGTTCAAA 
CCGCCGCCAG GATTTTCGGC CAGCATGGTC ACCGGTGCTC TAGCCAAGAA CAAGCGCGCT 
ACAGTTAAAC CCGTTACTGG CAACCTGGGC TTCGGCCAGC AATCGCAGGG TGGCTCTTAC 
AAGCACAGCC AGAACGTGAA TCCTAGTGCG GTATCCACCA TTGCACGTGA AGTAAACGCT 
GCTAAGAAGC AGGAGTTTAC CTGCAACCCC GCTTTGTACG GCTCTGCCTT AGAATCGCTT 
TCCAATTCAA GTCGCTCGAG CGAAAAGACT AACTCTACGG CAGGAGACAG TGAGTTCATA 
ATTGTCGCGA GCGAGGAGGA AGAGCAGGCT GCTTCCTCTG AGGAGCAACG CGAAGAAGCT 
GAAGAGGAGG AGTCCGGGGA ACGTGAAAGG GAGAAAGGCG AGCGCAACGC CACTGAAGCT 
ACCAAAGTCG AGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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TrpRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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