Wars2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wars2
Entrez Gene ID	690654
Full Name	tryptophanyl tRNA synthetase 2 (mitochondrial)
General protein information	Preferred Names tryptophanyl tRNA synthetase 2 (mitochondrial) Names tryptophan--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001168641.1	NP_001162112.1	tryptophan--tRNA ligase, mitochondrial
XM_006233028.3	XP_006233090.1	tryptophan--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WARS2	XP_416536.4
Eremothecium gossypii	AGOS_ACR089C	NP_983491.1
Rattus norvegicus (Norway rat)	Wars2	NP_001162112.1
Kluyveromyces lactis	KLLA0E21825g	XP_454939.1
Arabidopsis thaliana (thale cress)	OVA4	NP_973533.1
Saccharomyces cerevisiae (baker's yeast)	MSW1	NP_010554.1
Oryza sativa (rice)	Os01g0743400	NP_001044218.1
Xenopus tropicalis (tropical clawed frog)	wars2	XP_004912356.1
Homo sapiens (human)	WARS2	NP_056651.1
	WARS2	XP_001113563.1
Bos taurus (cattle)	WARS2	NP_001029754.1
Mus musculus (house mouse)	Wars2	NP_081738.2
Drosophila melanogaster (fruit fly)	CG7441	NP_649018.2
Schizosaccharomyces pombe (fission yeast)	msw1	NP_594085.1
Magnaporthe oryzae (rice blast fungus)	MGG_14679	XP_003718589.1
Pan troglodytes (chimpanzee)	WARS2	XP_003308338.2
Canis lupus familiaris (dog)	WARS2	XP_005630737.1
Danio rerio (zebrafish)	wars2	NP_001013341.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011744	XP_320768.4
Caenorhabditis elegans (roundworm)	prx-10	NP_001021201.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity.
Pravenec M, Z?dek V, Landa V, Mlejnek P, ?ilhav? J, ?im?kov? M, Trnovsk? J, ?kop V, Markov? I, Mal?nsk? H, H?ttl M, Kazdov? L, Bardov? K, Tauchmannov? K, Vrback? M, N?skov? H, Mr??ek T, Kopeck? J, Hou?t?k J
Physiological research66(6)917-924(2017 Dec)

Mutations of the aminoacyl-tRNA-synthetases SARS and WARS2 are implicated in the etiology of autosomal recessive intellectual disability.
Musante L, P?ttmann L, Kahrizi K, Garshasbi M, Hu H, Stehr H, Lipkowitz B, Otto S, Jensen LR, Tzschach A, Jamali P, Wienker T, Najmabadi H, Ropers HH, Kuss AW
Human mutation38(6)621-636(2017 Jun)

Wars2 is a determinant of angiogenesis.
Wang M, Sips P, Khin E, Rotival M, Sun X, Ahmed R, Widjaja AA, Schafer S, Yusoff P, Choksi PK, Ko NS, Singh MK, Epstein D, Guan Y, Hou?t?k J, Mracek T, Nuskova H, Mikell B, Tan J, Pesce F, Kolar F, Bottolo L, Mancini M, Hubner N, Pravenec M, Petretto E, MacRae C, Cook SA
Nature communications712061(2016 Jul)

T2-TrpRS inhibits preretinal neovascularization and enhances physiological vascular regrowth in OIR as assessed by a new method of quantification.
Banin E, Dorrell MI, Aguilar E, Ritter MR, Aderman CM, Smith AC, Friedlander J, Friedlander M
Investigative ophthalmology & visual science47(5)2125-34(2006 May)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05991	NM_001168641.1 Latest version!	Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial) (Wars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa25105	XM_006233028.3 Latest version!	Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial) (Wars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wars2 ( NM_001168641.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001162112.1 Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial) (Wars2), mRNA.

Price & Availability↑

CloneID	ORa05991
Clone ID Related Accession (Same CDS sequence)	NM_001168641.1
Accession Version	NM_001168641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-25
Organism	Rattus norvegicus(Norway rat)
Product	tryptophan--tRNA ligase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07012640.1 and AABR07012643.1. On or before Dec 13, 2009 this sequence version replaced XM_001065089.1, XM_001075111.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ081211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGCTGT TCTCCGTGCG GAAGGCGCGT GAGTGCTGGC GCTTCATCCG GGCACTTCAT 
AAAGGACCCA CAGCTACTGC GGCTCCCCAG AAGGAGAGCG GGGAGCGAGT GTTCTCTGGC 
ATTCAGCCTA CAGGAATCCC CCACCTGGGA AATTACCTTG GAGCCATCGA GAGCTGGGTG 
AAGTTACAGG AGGAGTATGA CACGGTGATA TACAGCATTG TGGACCTCCA CTCCATCACT 
GTCCCCCAAG ACCCCGGCAT CCTGCAGCAG AGCATCCTGG ACATGACTGC CGTGCTCCTT 
GCCTGCGGCA TAGACCCAGA GAGAAGTATC CTTTTCCAGC AGTCTCAGGT GTCTGAACAC 
ACTCAGTTAA GTTGGATCCT CACCTGCATG GTGAGACTGC CTCGACTACA GCATTTACAC 
CAGTGGAAGG CGAAGGCTGC GAGGCAGAAG CATGATGGGA CAGTAGGCCT GCTCACATAC 
CCTGTACTCC AGGCGGCAGA CATTCTGTGC TACAAGTCCA CACACGTTCC TGTCGGGGAA 
GATCAAGTCC AGCACATGGA ACTAGTTCAG GATCTAGCTC GGAGTTTCAA CCAGAAGTAT 
GGCGAGCTCT TTCCACTGCC CAGGTCTATT CTCACATCCA TGAAGAAAGT GAAATCTCTT 
CGTGACCCTT CTGCCAAGAT GTCAAAATCG GACCCTGACA AACTGGCCAC TGTCCAAATA 
ACAGACAGCC CAGAGGAGAT TGTACGGAAA TTCCGCAAGG CTGTGACGGA CTTCACGTCA 
GAAGTCACCT ACGAGCCGGA CAGCAGACCT GGTGTCTCCA ACATGGTGGC GATCCATGCG 
GCCGTGTCAG GCCTCTCGGT GGAGGAGGTG GTGCGCAATA GTGCAGGCGT GGACACTGCA 
CGCTACAAGC TGCTAGTGGC CGACGCTGTG ATTGAGAAAT TTGCTCCAAT CAGAAGTGAA 
ATTGAAAAAC TGAAAATGGA TAAGGACCAT TTAAGAAAGG TTTTACTTGT GGGATCTGCA 
AAAGCCAAAG AATTAGCCTC TCCTGTGTTC GAGGAGGTGA AGAAGTTGGT GGGGATTCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162112.1
CDS	11..1093
Translation	MALFSVRKARECWRFIRALHKGPTATAAPQKESGERVFSGIQPTGIPHLGNYLGAIESWVKLQEEYDTVIYSIVDLHSITVPQDPGILQQSILDMTAVLLACGIDPERSILFQQSQVSEHTQLSWILTCMVRLPRLQHLHQWKAKAARQKHDGTVGLLTYPVLQAADILCYKSTHVPVGEDQVQHMELVQDLARSFNQKYGELFPLPRSILTSMKKVKSLRDPSAKMSKSDPDKLATVQITDSPEEIVRKFRKAVTDFTSEVTYEPDSRPGVSNMVAIHAAVSGLSVEEVVRNSAGVDTARYKLLVADAVIEKFAPIRSEIEKLKMDKDHLRKVLLVGSAKAKELASPVFEEVKKLVGIL

Target ORF information:

RefSeq Version	NM_001168641.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial) (Wars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168641.1

ORF Insert Sequence:

ATGGCGCTGT TCTCCGTGCG GAAGGCGCGT GAGTGCTGGC GCTTCATCCG GGCACTTCAT 
AAAGGACCCA CAGCTACTGC GGCTCCCCAG AAGGAGAGCG GGGAGCGAGT GTTCTCTGGC 
ATTCAGCCTA CAGGAATCCC CCACCTGGGA AATTACCTTG GAGCCATCGA GAGCTGGGTG 
AAGTTACAGG AGGAGTATGA CACGGTGATA TACAGCATTG TGGACCTCCA CTCCATCACT 
GTCCCCCAAG ACCCCGGCAT CCTGCAGCAG AGCATCCTGG ACATGACTGC CGTGCTCCTT 
GCCTGCGGCA TAGACCCAGA GAGAAGTATC CTTTTCCAGC AGTCTCAGGT GTCTGAACAC 
ACTCAGTTAA GTTGGATCCT CACCTGCATG GTGAGACTGC CTCGACTACA GCATTTACAC 
CAGTGGAAGG CGAAGGCTGC GAGGCAGAAG CATGATGGGA CAGTAGGCCT GCTCACATAC 
CCTGTACTCC AGGCGGCAGA CATTCTGTGC TACAAGTCCA CACACGTTCC TGTCGGGGAA 
GATCAAGTCC AGCACATGGA ACTAGTTCAG GATCTAGCTC GGAGTTTCAA CCAGAAGTAT 
GGCGAGCTCT TTCCACTGCC CAGGTCTATT CTCACATCCA TGAAGAAAGT GAAATCTCTT 
CGTGACCCTT CTGCCAAGAT GTCAAAATCG GACCCTGACA AACTGGCCAC TGTCCAAATA 
ACAGACAGCC CAGAGGAGAT TGTACGGAAA TTCCGCAAGG CTGTGACGGA CTTCACGTCA 
GAAGTCACCT ACGAGCCGGA CAGCAGACCT GGTGTCTCCA ACATGGTGGC GATCCATGCG 
GCCGTGTCAG GCCTCTCGGT GGAGGAGGTG GTGCGCAATA GTGCAGGCGT GGACACTGCA 
CGCTACAAGC TGCTAGTGGC CGACGCTGTG ATTGAGAAAT TTGCTCCAAT CAGAAGTGAA 
ATTGAAAAAC TGAAAATGGA TAAGGACCAT TTAAGAAAGG TTTTACTTGT GGGATCTGCA 
AAAGCCAAAG AATTAGCCTC TCCTGTGTTC GAGGAGGTGA AGAAGTTGGT GGGGATTCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wars2 ( XM_006233028.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233090.1 Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial) (Wars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25105
Clone ID Related Accession (Same CDS sequence)	XM_006233028.3
Accession Version	XM_006233028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tryptophan--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCTCCGTGCG GAAGGCGCGT GAGTGCTGGC GCTTCATCCG GGCACTTCAT 
AAAGGACCCA CAGCTACTGC GGCTCCCCAG AAGGAGAGCG GGGAGCGAGT GTTCTCTGGC 
ATTCAGCCTA CAGGAATCCC CCACCTGGGA AATTACCTTG GAGCCATCGA GAGCTGGGTG 
AAGTTACAGG AGGAGTATGA CACGGTGATA TACAGCATTG TGGACCTCCA CTCCATCACT 
GTCCCCCAAG ACCCCGGCAT CCTGCAGCAG AGCATCCTGG ACATGACTGC CGTGCTCCTT 
GCCTGCGGCA TAGACCCAGA GAGAAGTATC CTTTTCCAGC AGTCTCAGGC GAAGGCTGCG 
AGGCAGAAGC ATGATGGGAC AGTAGGCCTG CTCACATACC CTGTACTCCA GGCGGCAGAC 
ATTCTGTGCT ACAAGTCCAC ACACGTTCCT GTCGGGGAAG ATCAAGTCCA GCACATGGAA 
CTAGTTCAGG ATCTAGCTCG GAGTTTCAAC CAGAAGTATG GCGAGCTCTT TCCACTGCCC 
AGGTCTATTC TCACATCCAT GAAGAAAGTG AAATCTCTTC GTGACCCTTC TGCCAAGATG 
TCAAAATCGG ACCCTGACAA ACTGGCCACT GTCCAAATAA CAGACAGCCC AGAGGAGATT 
GTACGGAAAT TCCGCAAGGC TGTGACGGAC TTCACGTCAG AAGTCACCTA CGAGCCGGAC 
AGCAGACCTG GTGTCTCCAA CATGGTGGCG ATCCATGCGG CCGTGTCAGG CCTCTCGGTG 
GAGGAGGTGG TGCGCAATAG TGCAGGCGTG GACACTGCAC GCTACAAGCT GCTAGTGGCC 
GACGCTGTGA TTGAGAAATT TGCTCCAATC AGAAGTGAAA TTGAAAAACT GAAAATGGAT 
AAGGACCATT TAAGAAAGGT TTTACTTGTG GGATCTGCAA AAGCCAAAGA ATTAGCCTCT 
CCTGTGTTCG AGGAGGTGAA GAAGTTGGTG GGGATTCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233090.1
CDS	1960..2961
Translation	MALFSVRKARECWRFIRALHKGPTATAAPQKESGERVFSGIQPTGIPHLGNYLGAIESWVKLQEEYDTVIYSIVDLHSITVPQDPGILQQSILDMTAVLLACGIDPERSILFQQSQAKAARQKHDGTVGLLTYPVLQAADILCYKSTHVPVGEDQVQHMELVQDLARSFNQKYGELFPLPRSILTSMKKVKSLRDPSAKMSKSDPDKLATVQITDSPEEIVRKFRKAVTDFTSEVTYEPDSRPGVSNMVAIHAAVSGLSVEEVVRNSAGVDTARYKLLVADAVIEKFAPIRSEIEKLKMDKDHLRKVLLVGSAKAKELASPVFEEVKKLVGIL

Target ORF information:

RefSeq Version	XM_006233028.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial) (Wars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233028.3

ORF Insert Sequence:

ATGGCGCTGT TCTCCGTGCG GAAGGCGCGT GAGTGCTGGC GCTTCATCCG GGCACTTCAT 
AAAGGACCCA CAGCTACTGC GGCTCCCCAG AAGGAGAGCG GGGAGCGAGT GTTCTCTGGC 
ATTCAGCCTA CAGGAATCCC CCACCTGGGA AATTACCTTG GAGCCATCGA GAGCTGGGTG 
AAGTTACAGG AGGAGTATGA CACGGTGATA TACAGCATTG TGGACCTCCA CTCCATCACT 
GTCCCCCAAG ACCCCGGCAT CCTGCAGCAG AGCATCCTGG ACATGACTGC CGTGCTCCTT 
GCCTGCGGCA TAGACCCAGA GAGAAGTATC CTTTTCCAGC AGTCTCAGGC GAAGGCTGCG 
AGGCAGAAGC ATGATGGGAC AGTAGGCCTG CTCACATACC CTGTACTCCA GGCGGCAGAC 
ATTCTGTGCT ACAAGTCCAC ACACGTTCCT GTCGGGGAAG ATCAAGTCCA GCACATGGAA 
CTAGTTCAGG ATCTAGCTCG GAGTTTCAAC CAGAAGTATG GCGAGCTCTT TCCACTGCCC 
AGGTCTATTC TCACATCCAT GAAGAAAGTG AAATCTCTTC GTGACCCTTC TGCCAAGATG 
TCAAAATCGG ACCCTGACAA ACTGGCCACT GTCCAAATAA CAGACAGCCC AGAGGAGATT 
GTACGGAAAT TCCGCAAGGC TGTGACGGAC TTCACGTCAG AAGTCACCTA CGAGCCGGAC 
AGCAGACCTG GTGTCTCCAA CATGGTGGCG ATCCATGCGG CCGTGTCAGG CCTCTCGGTG 
GAGGAGGTGG TGCGCAATAG TGCAGGCGTG GACACTGCAC GCTACAAGCT GCTAGTGGCC 
GACGCTGTGA TTGAGAAATT TGCTCCAATC AGAAGTGAAA TTGAAAAACT GAAAATGGAT 
AAGGACCATT TAAGAAAGGT TTTACTTGTG GGATCTGCAA AAGCCAAAGA ATTAGCCTCT 
CCTGTGTTCG AGGAGGTGAA GAAGTTGGTG GGGATTCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity.
Physiological research66(6)917-924(2017 Dec)
Pravenec M,Z?dek V,Landa V,Mlejnek P,?ilhav? J,?im?kov? M,Trnovsk? J,?kop V,Markov? I,Mal?nsk? H,H?ttl M,Kazdov? L,Bardov? K,Tauchmannov? K,Vrback? M,N?skov? H,Mr??ek T,Kopeck? J,Hou?t?k J

Mutations of the aminoacyl-tRNA-synthetases SARS and WARS2 are implicated in the etiology of autosomal recessive intellectual disability.
Human mutation38(6)621-636(2017 Jun)
Musante L,P?ttmann L,Kahrizi K,Garshasbi M,Hu H,Stehr H,Lipkowitz B,Otto S,Jensen LR,Tzschach A,Jamali P,Wienker T,Najmabadi H,Ropers HH,Kuss AW

Wars2 is a determinant of angiogenesis.
Nature communications712061(2016 Jul)
Wang M,Sips P,Khin E,Rotival M,Sun X,Ahmed R,Widjaja AA,Schafer S,Yusoff P,Choksi PK,Ko NS,Singh MK,Epstein D,Guan Y,Hou?t?k J,Mracek T,Nuskova H,Mikell B,Tan J,Pesce F,Kolar F,Bottolo L,Mancini M,Hubner N,Pravenec M,Petretto E,MacRae C,Cook SA

T2-TrpRS inhibits preretinal neovascularization and enhances physiological vascular regrowth in OIR as assessed by a new method of quantification.
Investigative ophthalmology & visual science47(5)2125-34(2006 May)
Banin E,Dorrell MI,Aguilar E,Ritter MR,Aderman CM,Smith AC,Friedlander J,Friedlander M

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Wars2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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