Eip74EF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eip74EF
Entrez Gene ID	39962
Full Name	Ecdysone-induced protein 74EF
Synonyms	3.3,74EF,CG32180,CG6273,CG6285,Dmel\CG32180,E-74,E74,E74-B,E74A,E74A/B,E74B,ECIP,Eip74,Eip74 A,Eip74A,Eip74E,e74,e74B,eip74EF,eip74ef,l(3)24,l(3)neo24,mE74
General protein information	Preferred Names Ecdysone-induced protein 74EF Names CG32180 gene product from transcript CG32180-RD CG32180-PA CG32180-PB CG32180-PC CG32180-PD CG32180-PE Ecdysone-induced prot 74EF Ecdysone-induced-protein 74EF Eip74EF-PA Eip74EF-PB Eip74EF-PC Eip74EF-PD Eip74EF-PE ecdysone induced protein 74EF ecdysone inducible protein ecdysone-induced protein 74EF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 74D4-74E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_168740.3	NP_730287.1	Ecdysone-induced protein 74EF, isoform A
NM_168739.3	NP_730286.3	Ecdysone-induced protein 74EF, isoform C
NM_168741.2	NP_730288.1	Ecdysone-induced protein 74EF, isoform B
NM_001014590.2	NP_001014590.1	Ecdysone-induced protein 74EF, isoform D
NM_001259887.2	NP_001246816.1	Ecdysone-induced protein 74EF, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mid-embryonic expression of DHR3 and betaFTZ-F1 is part of a 20-hydroxyecdysone (20E)-triggered transcriptional cascade, directing maximal expression of E74A and E75A during late embryogenesis.
Title: The Drosophila nuclear receptors DHR3 and betaFTZ-F1 control overlapping developmental responses in late embryos.

LARK RNA-binding protein selectively regulates the circadian eclosion rhythm by controlling E74 protein expression
Title: The LARK RNA-binding protein selectively regulates the circadian eclosion rhythm by controlling E74 protein expression.

mutations in the ecdysone-regulated E74A and E74B genes do not appear to perturb midgut development during metamorphosis
Title: Steroid regulation of midgut cell death during Drosophila development.

The following Eip74EF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eip74EF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18839	NM_001259887.2 Latest version!	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa21021	NM_168741.2 Latest version!	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa21021	NM_001014590.2 Latest version!	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa21022	NM_168740.3 Latest version!	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa21023	NM_168739.3 Latest version!	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eip74EF ( NM_001259887.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246816.1 Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa18839
Clone ID Related Accession (Same CDS sequence)	NM_001259887.2
Accession Version	NM_001259887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 74EF, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259887.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

CTGGCAGTTA ACAGTGTTTG CAGTGCAGGC TTTGCCCTAT CAGCGATGCC CTTTATCGAC 
GATGCACTGC TCTGGTGTCC AGATAACGAT GGTCGTCTTG TTGGAGGTCT AGACTTGGGA 
ACATGCATAG CCGATGATTC TACGGCTAAC GGAACGGAAA ACTTGAACCC CTCGATCCAA 
TCAGCTGGCA ATCCGAACAA CCCGCAGCAG AGCGTCGGTG GCGAGATCCT GGGATCCGTG 
GAGTCCGCCG GCAACGAACT GAATGGAGCG GCGGCCCGCA ACGTGAACGT GGTGGTGGAA 
CCGCTCTGCG GCGGCGACTC CTCCGACGAG CTGTTCCGCA GCTTCTCCGA GAGCAACTTC 
GAGATCGAGA GCCTGCTGTC CGATTTGGCC ACCGTCGAGG TGAAAGTCGA AAATGAGGAG 
AACAACAACA ATGTCATCAC CGATGATGAC TTCGCCAGCG TCGCTGCCGC CGTGGTGGCC 
AACGATGATC TGCTAGCCAA AGAGAATGCC CAGCTGAGTG CCCAGGGTCT GGTCGATTCG 
GTGGCCGCCA GTCTGGCCGA TTCCGGCGAT GCCGGTGGTC AGCAGGCGCT ACTGGCCTTC 
GGCTCTTCCA GCTCGGCAGC CTCGGCGATT GCAGCAGCAG CTGCCGCCCT CTGTGGCGAT 
TTGATCAACA ACAACAACAA CAACAGCAAC AGCAACAACA ACAGCAATGG CAATGGCAAT 
CACGGCGGCG GGGGAGGAGG AGCTAGTTCC GGCGGCGGAG TAGCCGGCGA CTGTGCCACC 
AAGCTGGAGT ACGCCCTCAT GGGCGGACAG CCGCTGGCGG AGGAGCCGCG CTTCGTGACC 
AGCGCCGCCG CCAATCCCCT TCTCGTCGAG AAGCTAATGT CCAAGTGCCT GAACATTGAG 
AAGCGAATGG ACAAGCTCAG CGACACCGAA ATTCCTATTG TCAAGCAATC GACAAGTCCG 
GCACCGCAGC AGCAACTGCA GCAGCAGCAC CACCTGCAAC AGCAGCAACA GCAGCAGCCG 
CACAACGGCT CCACCTTCGC CGGCGCCACC GCTCTGCTCC ACATAAAGAC GGAGCAAAAT 
ACGCTGCTCA CTCCGCTCCA GCTGCAGCAG CAGCAACAAC AGCAGCAGGG TCTACACGGT 
GCGGCAGGCA ACGGAGGCAG CAGCAACGGC AACAATGCCC ACCAGCAGCA ACAGCCGCTG 
GCCATCCCAC AACGCCCGCT GCTCCACAAT CTGCTTAGCG GCGGTGCCAT CCACAATCCG 
CACCACAGAA ACTACACGAC GGCCACAACA GGTTCATTTC CGCCCAGTCC CGCGGACAGC 
GGCGTCTCCG ACGTGGACAG CTCCAGTTCC GGCGGCCAGC CCTGCGCCGA CGAGCTGAAG 
GCGCGTCTCG GGATGCCACC AGCCACCTCG GCGTCGGCAG CGGCCGCGGC AGCAGCGGCG 
GCGGCGGCAG CGGCACACCT GCACACGGGC ACCTTTCTGC ATCCGAATTT ATACCAGAAC 
AATGCGGCAA ATTCGCTGCG GAACATATGG AATCGCAGTG TTGGGGTGCC CGACAACTAC 
TACGGAAGCA GTGGCGCCGG CAGTGGGGGC ACCCAGCCAG GTGGTCCGGG AAACCCCCAA 
ACGCCTGGCT ACCTGACAAC GTCCTACTTC AACGCACCGA CAGCGGCAAC GGCAGCGGCG 
TCCCAGCGAG GAACCACAAT CAATGGCTAT CATTCCCTGC ACCAGCAGCA GCAGCAGCAA 
CAGCAGTCGC AGCAATCGCA GCAGCAACAG CAACTGGCCC ACCAGCAACT GTCGCACCAG 
CAGCAGCAGG CGTTGCATCA GCAACTGTCG CACCAACAGC AGCAGCAGCA ACAGCAACAG 
CAGCAACACC CGCACTCCCA ACTGAACGGT CCGCATCCGC ACTCACATCC GCACTCTCAT 
CCGCATTCGC ATCCGCACGC GGGCCAGCAC ACGCATTCCA CAATCGCTGC AGCGGCGGCA 
GCGGCAGCAG CATCTGTCGT CAGCAGCAGC AGCAGCGCCG TTGCAGCGGC AGCCATGCTG 
AGCGCCAGTG CAGCGGCAGC AGCAACGGCG GCAGCTGCGG CGGGCGGATC CCAGAGTGTT 
ATCCAACCGG CGACGTCCAG CGTCAGCTAC GATCTCTCCT ACATGCTGGA GCTGGGCGGA 
TTCCAGCAGC GGAAGGCGAA GAAGCCGCGC AAACCGAAGC TGGAGATGGG CGTAAAGCGG 
CGCAGCCGGG AGGGATCCAC CACCTATCTG TGGGAGTTCC TCCTCAAACT GCTCCAGGAT 
CGCGAATACT GTCCGCGTTT CATCAAGTGG ACGAACCGGG AGAAGGGCGT CTTCAAGCTG 
GTCGACTCGA AGGCTGTGTC CCGCCTGTGG GGCATGCACA AGAACAAGCC GGACATGAAC 
TACGAGACGA TGGGCAGGGC GCTGAGGTAC TACTACCAGC GCGGCATACT GGCCAAGGTG 
GATGGCCAGC GGCTGGTCTA CCAGTTCGTG GATGTGCCCA AGGACATCAT CGAGATTGAC 
TGCAACGGTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246816.1
CDS	1866..4400
Translation	LAVNSVCSAGFALSAMPFIDDALLWCPDNDGRLVGGLDLGTCIADDSTANGTENLNPSIQSAGNPNNPQQSVGGEILGSVESAGNELNGAAARNVNVVVEPLCGGDSSDELFRSFSESNFEIESLLSDLATVEVKVENEENNNNVITDDDFASVAAAVVANDDLLAKENAQLSAQGLVDSVAASLADSGDAGGQQALLAFGSSSSAASAIAAAAAALCGDLINNNNNNSNSNNNSNGNGNHGGGGGGASSGGGVAGDCATKLEYALMGGQPLAEEPRFVTSAAANPLLVEKLMSKCLNIEKRMDKLSDTEIPIVKQSTSPAPQQQLQQQHHLQQQQQQQPHNGSTFAGATALLHIKTEQNTLLTPLQLQQQQQQQQGLHGAAGNGGSSNGNNAHQQQQPLAIPQRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPPATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGAGSGGTQPGGPGNPQTPGYLTTSYFNAPTAATAAASQRGTTINGYHSLHQQQQQQQQSQQSQQQQQLAHQQLSHQQQQALHQQLSHQQQQQQQQQQQHPHSQLNGPHPHSHPHSHPHSHPHAGQHTHSTIAAAAAAAAASVVSSSSSAVAAAAMLSASAAAAATAAAAAGGSQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIIEIDCNGV

Target ORF information:

RefSeq Version	NM_001259887.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259887.2

ORF Insert Sequence:

CTGGCAGTTA ACAGTGTTTG CAGTGCAGGC TTTGCCCTAT CAGCGATGCC CTTTATCGAC 
GATGCACTGC TCTGGTGTCC AGATAACGAT GGTCGTCTTG TTGGAGGTCT AGACTTGGGA 
ACATGCATAG CCGATGATTC TACGGCTAAC GGAACGGAAA ACTTGAACCC CTCGATCCAA 
TCAGCTGGCA ATCCGAACAA CCCGCAGCAG AGCGTCGGTG GCGAGATCCT GGGATCCGTG 
GAGTCCGCCG GCAACGAACT GAATGGAGCG GCGGCCCGCA ACGTGAACGT GGTGGTGGAA 
CCGCTCTGCG GCGGCGACTC CTCCGACGAG CTGTTCCGCA GCTTCTCCGA GAGCAACTTC 
GAGATCGAGA GCCTGCTGTC CGATTTGGCC ACCGTCGAGG TGAAAGTCGA AAATGAGGAG 
AACAACAACA ATGTCATCAC CGATGATGAC TTCGCCAGCG TCGCTGCCGC CGTGGTGGCC 
AACGATGATC TGCTAGCCAA AGAGAATGCC CAGCTGAGTG CCCAGGGTCT GGTCGATTCG 
GTGGCCGCCA GTCTGGCCGA TTCCGGCGAT GCCGGTGGTC AGCAGGCGCT ACTGGCCTTC 
GGCTCTTCCA GCTCGGCAGC CTCGGCGATT GCAGCAGCAG CTGCCGCCCT CTGTGGCGAT 
TTGATCAACA ACAACAACAA CAACAGCAAC AGCAACAACA ACAGCAATGG CAATGGCAAT 
CACGGCGGCG GGGGAGGAGG AGCTAGTTCC GGCGGCGGAG TAGCCGGCGA CTGTGCCACC 
AAGCTGGAGT ACGCCCTCAT GGGCGGACAG CCGCTGGCGG AGGAGCCGCG CTTCGTGACC 
AGCGCCGCCG CCAATCCCCT TCTCGTCGAG AAGCTAATGT CCAAGTGCCT GAACATTGAG 
AAGCGAATGG ACAAGCTCAG CGACACCGAA ATTCCTATTG TCAAGCAATC GACAAGTCCG 
GCACCGCAGC AGCAACTGCA GCAGCAGCAC CACCTGCAAC AGCAGCAACA GCAGCAGCCG 
CACAACGGCT CCACCTTCGC CGGCGCCACC GCTCTGCTCC ACATAAAGAC GGAGCAAAAT 
ACGCTGCTCA CTCCGCTCCA GCTGCAGCAG CAGCAACAAC AGCAGCAGGG TCTACACGGT 
GCGGCAGGCA ACGGAGGCAG CAGCAACGGC AACAATGCCC ACCAGCAGCA ACAGCCGCTG 
GCCATCCCAC AACGCCCGCT GCTCCACAAT CTGCTTAGCG GCGGTGCCAT CCACAATCCG 
CACCACAGAA ACTACACGAC GGCCACAACA GGTTCATTTC CGCCCAGTCC CGCGGACAGC 
GGCGTCTCCG ACGTGGACAG CTCCAGTTCC GGCGGCCAGC CCTGCGCCGA CGAGCTGAAG 
GCGCGTCTCG GGATGCCACC AGCCACCTCG GCGTCGGCAG CGGCCGCGGC AGCAGCGGCG 
GCGGCGGCAG CGGCACACCT GCACACGGGC ACCTTTCTGC ATCCGAATTT ATACCAGAAC 
AATGCGGCAA ATTCGCTGCG GAACATATGG AATCGCAGTG TTGGGGTGCC CGACAACTAC 
TACGGAAGCA GTGGCGCCGG CAGTGGGGGC ACCCAGCCAG GTGGTCCGGG AAACCCCCAA 
ACGCCTGGCT ACCTGACAAC GTCCTACTTC AACGCACCGA CAGCGGCAAC GGCAGCGGCG 
TCCCAGCGAG GAACCACAAT CAATGGCTAT CATTCCCTGC ACCAGCAGCA GCAGCAGCAA 
CAGCAGTCGC AGCAATCGCA GCAGCAACAG CAACTGGCCC ACCAGCAACT GTCGCACCAG 
CAGCAGCAGG CGTTGCATCA GCAACTGTCG CACCAACAGC AGCAGCAGCA ACAGCAACAG 
CAGCAACACC CGCACTCCCA ACTGAACGGT CCGCATCCGC ACTCACATCC GCACTCTCAT 
CCGCATTCGC ATCCGCACGC GGGCCAGCAC ACGCATTCCA CAATCGCTGC AGCGGCGGCA 
GCGGCAGCAG CATCTGTCGT CAGCAGCAGC AGCAGCGCCG TTGCAGCGGC AGCCATGCTG 
AGCGCCAGTG CAGCGGCAGC AGCAACGGCG GCAGCTGCGG CGGGCGGATC CCAGAGTGTT 
ATCCAACCGG CGACGTCCAG CGTCAGCTAC GATCTCTCCT ACATGCTGGA GCTGGGCGGA 
TTCCAGCAGC GGAAGGCGAA GAAGCCGCGC AAACCGAAGC TGGAGATGGG CGTAAAGCGG 
CGCAGCCGGG AGGGATCCAC CACCTATCTG TGGGAGTTCC TCCTCAAACT GCTCCAGGAT 
CGCGAATACT GTCCGCGTTT CATCAAGTGG ACGAACCGGG AGAAGGGCGT CTTCAAGCTG 
GTCGACTCGA AGGCTGTGTC CCGCCTGTGG GGCATGCACA AGAACAAGCC GGACATGAAC 
TACGAGACGA TGGGCAGGGC GCTGAGGTAC TACTACCAGC GCGGCATACT GGCCAAGGTG 
GATGGCCAGC GGCTGGTCTA CCAGTTCGTG GATGTGCCCA AGGACATCAT CGAGATTGAC 
TGCAACGGTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip74EF ( NM_168741.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730288.1 Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21021
Clone ID Related Accession (Same CDS sequence)	NM_168741.2 , NM_001014590.2
Accession Version	NM_168741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 74EF, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Apr 11, 2005 this sequence version replaced NM_168741.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATGG TGCAACATTT GGTGGCCGCC TCGGCGCACA ACTTCGCCAG CCAGGCGGCG 
GCATCGCTTG TCAATGTGTC CAGCTCCAGC TCTAGTTCCA GTTCGAGTTC CTCGTCCTCG 
CTGTCGCTGT CTTCATCCTC ATCCAGCTCC TCGCTGTCCT CGGCCACGCC CACGCCGGTG 
GCCTCGCCCG TGACGCCCAC ATCTCCACCG CCGGCAGCCG CCGCACCCGC AGAAGCCTCG 
CCGCCAGCAG GAGCTGAGTT GCAGGAGGAT GGGCAGCAGG CTAAGACTCA GGAGGATCCT 
ACTATGAAGG ACCAAGACAT GCTGGAAAAG ACGAGGCAAG AGGTGAAGGA TCCAGTTAAT 
GTGGAGGAAC CAGGTGCCAT AGTTGATACC GAGTCAGTGA TGGCGCGGCA AAGTCCAAGT 
CCAGTTGCGT CCACCAAAGT CCCCGAGTCA CTAGAAGAGA TTTCCAACAA ATCGCCGCCC 
GTTCAAGAGG ATGAAGAGGA GTCTGAATCC GTAGCCTCCG ACTGTCGCGA GTTCAAAGTG 
CTCTATAATC ACCTAAGGCA GCAGCAACAC CACCACTCTC CCTCCTCGCC AGACAAGACG 
CGCAGCACCC TGGACGATGT GTCCAAGATC CTCTGGGAGC GAAAGCAGCA GCTGCAGCGC 
AGCTCAGTGA TTACCGCAGC ACCCACTCTG CAGCCGCAGC AGCACCAGCA GCCCATGAGC 
GACATCGAGG ACGAGGAGAC GCTGGAGGAT GTCGATGACG CGGATGCGGA CGTGGAGGCA 
GACGCCGAGG ACGAGGAGCT ACTGGAGCAG TACCAAAACG GCTACGACTC GCCACTGGAT 
CTGTCCCTGG GTGGAGCCAC CAGTGCAGCC GCATCAGCTG CCGCTGCCGC CTCGGCGGTC 
AGTCGCCGAC GGGGACGCAC CTACTCCGGC ACGGAATCCG ACGACTCCGC GCAGTGCGAA 
CGGGCGAGGA TGCGACTCAA GCCGGAACGC AAGGCGGAGA GATCGGCGGC CTACAAGAAG 
AGCTTGATGA AGCGATATTA CACCGAAATT CCTATTGTCA AGCAATCGAC AAGTCCGGCA 
CCGCAGCAGC AACTGCAGCA GCAGCACCAC CTGCAACAGC AGCAACAGCA GCAGCCGCAC 
AACGGCTCCA CCTTCGCCGG CGCCACCGCT CTGCTCCACA TAAAGACGGA GCAAAATACG 
CTGCTCACTC CGCTCCAGCT GCAGCAGCAG CAACAACAGC AGCAGGGTCT ACACGGTGCG 
GCAGGCAACG GAGGCAGCAG CAACGGCAAC AATGCCCACC AGCAGCAACA GCCGCTGGCC 
ATCCCACAAC GCCCGCTGCT CCACAATCTG CTTAGCGGCG GTGCCATCCA CAATCCGCAC 
CACAGAAACT ACACGACGGC CACAACAGGT TCATTTCCGC CCAGTCCCGC GGACAGCGGC 
GTCTCCGACG TGGACAGCTC CAGTTCCGGC GGCCAGCCCT GCGCCGACGA GCTGAAGGCG 
CGTCTCGGGA TGCCACCAGC CACCTCGGCG TCGGCAGCGG CCGCGGCAGC AGCGGCGGCG 
GCGGCAGCGG CACACCTGCA CACGGGCACC TTTCTGCATC CGAATTTATA CCAGAACAAT 
GCGGCAAATT CGCTGCGGAA CATATGGAAT CGCAGTGTTG GGGTGCCCGA CAACTACTAC 
GGAAGCAGTG GCGCCGGCAG TGGGGGCACC CAGCCAGGTG GTCCGGGAAA CCCCCAAACG 
CCTGGCTACC TGACAACGTC CTACTTCAAC GCACCGACAG CGGCAACGGC AGCGGCGTCC 
CAGCGAGGAA CCACAATCAA TGGCTATCAT TCCCTGCACC AGCAGCAGCA GCAGCAACAG 
CAGTCGCAGC AATCGCAGCA GCAACAGCAA CTGGCCCACC AGCAACTGTC GCACCAGCAG 
CAGCAGGCGT TGCATCAGCA ACTGTCGCAC CAACAGCAGC AGCAGCAACA GCAACAGCAG 
CAACACCCGC ACTCCCAACT GAACGGTCCG CATCCGCACT CACATCCGCA CTCTCATCCG 
CATTCGCATC CGCACGCGGG CCAGCACACG CATTCCACAA TCGCTGCAGC GGCGGCAGCG 
GCAGCAGCAT CTGTCGTCAG CAGCAGCAGC AGCGCCGTTG CAGCGGCAGC CATGCTGAGC 
GCCAGTGCAG CGGCAGCAGC AACGGCGGCA GCTGCGGCGG GCGGATCCCA GAGTGTTATC 
CAACCGGCGA CGTCCAGCGT CAGCTACGAT CTCTCCTACA TGCTGGAGCT GGGCGGATTC 
CAGCAGCGGA AGGCGAAGAA GCCGCGCAAA CCGAAGCTGG AGATGGGCGT AAAGCGGCGC 
AGCCGGGAGG GATCCACCAC CTATCTGTGG GAGTTCCTCC TCAAACTGCT CCAGGATCGC 
GAATACTGTC CGCGTTTCAT CAAGTGGACG AACCGGGAGA AGGGCGTCTT CAAGCTGGTC 
GACTCGAAGG CTGTGTCCCG CCTGTGGGGC ATGCACAAGA ACAAGCCGGA CATGAACTAC 
GAGACGATGG GCAGGGCGCT GAGGTACTAC TACCAGCGCG GCATACTGGC CAAGGTGGAT 
GGCCAGCGGC TGGTCTACCA GTTCGTGGAT GTGCCCAAGG ACATCATCGA GATTGACTGC 
AACGGTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730288.1
CDS	796..3447
Translation	MIMVQHLVAASAHNFASQAAASLVNVSSSSSSSSSSSSSSLSLSSSSSSSSLSSATPTPVASPVTPTSPPPAAAAPAEASPPAGAELQEDGQQAKTQEDPTMKDQDMLEKTRQEVKDPVNVEEPGAIVDTESVMARQSPSPVASTKVPESLEEISNKSPPVQEDEEESESVASDCREFKVLYNHLRQQQHHHSPSSPDKTRSTLDDVSKILWERKQQLQRSSVITAAPTLQPQQHQQPMSDIEDEETLEDVDDADADVEADAEDEELLEQYQNGYDSPLDLSLGGATSAAASAAAAASAVSRRRGRTYSGTESDDSAQCERARMRLKPERKAERSAAYKKSLMKRYYTEIPIVKQSTSPAPQQQLQQQHHLQQQQQQQPHNGSTFAGATALLHIKTEQNTLLTPLQLQQQQQQQQGLHGAAGNGGSSNGNNAHQQQQPLAIPQRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPPATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGAGSGGTQPGGPGNPQTPGYLTTSYFNAPTAATAAASQRGTTINGYHSLHQQQQQQQQSQQSQQQQQLAHQQLSHQQQQALHQQLSHQQQQQQQQQQQHPHSQLNGPHPHSHPHSHPHSHPHAGQHTHSTIAAAAAAAAASVVSSSSSAVAAAAMLSASAAAAATAAAAAGGSQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIIEIDCNGV

Target ORF information:

RefSeq Version	NM_168741.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168741.2

ORF Insert Sequence:

ATGATTATGG TGCAACATTT GGTGGCCGCC TCGGCGCACA ACTTCGCCAG CCAGGCGGCG 
GCATCGCTTG TCAATGTGTC CAGCTCCAGC TCTAGTTCCA GTTCGAGTTC CTCGTCCTCG 
CTGTCGCTGT CTTCATCCTC ATCCAGCTCC TCGCTGTCCT CGGCCACGCC CACGCCGGTG 
GCCTCGCCCG TGACGCCCAC ATCTCCACCG CCGGCAGCCG CCGCACCCGC AGAAGCCTCG 
CCGCCAGCAG GAGCTGAGTT GCAGGAGGAT GGGCAGCAGG CTAAGACTCA GGAGGATCCT 
ACTATGAAGG ACCAAGACAT GCTGGAAAAG ACGAGGCAAG AGGTGAAGGA TCCAGTTAAT 
GTGGAGGAAC CAGGTGCCAT AGTTGATACC GAGTCAGTGA TGGCGCGGCA AAGTCCAAGT 
CCAGTTGCGT CCACCAAAGT CCCCGAGTCA CTAGAAGAGA TTTCCAACAA ATCGCCGCCC 
GTTCAAGAGG ATGAAGAGGA GTCTGAATCC GTAGCCTCCG ACTGTCGCGA GTTCAAAGTG 
CTCTATAATC ACCTAAGGCA GCAGCAACAC CACCACTCTC CCTCCTCGCC AGACAAGACG 
CGCAGCACCC TGGACGATGT GTCCAAGATC CTCTGGGAGC GAAAGCAGCA GCTGCAGCGC 
AGCTCAGTGA TTACCGCAGC ACCCACTCTG CAGCCGCAGC AGCACCAGCA GCCCATGAGC 
GACATCGAGG ACGAGGAGAC GCTGGAGGAT GTCGATGACG CGGATGCGGA CGTGGAGGCA 
GACGCCGAGG ACGAGGAGCT ACTGGAGCAG TACCAAAACG GCTACGACTC GCCACTGGAT 
CTGTCCCTGG GTGGAGCCAC CAGTGCAGCC GCATCAGCTG CCGCTGCCGC CTCGGCGGTC 
AGTCGCCGAC GGGGACGCAC CTACTCCGGC ACGGAATCCG ACGACTCCGC GCAGTGCGAA 
CGGGCGAGGA TGCGACTCAA GCCGGAACGC AAGGCGGAGA GATCGGCGGC CTACAAGAAG 
AGCTTGATGA AGCGATATTA CACCGAAATT CCTATTGTCA AGCAATCGAC AAGTCCGGCA 
CCGCAGCAGC AACTGCAGCA GCAGCACCAC CTGCAACAGC AGCAACAGCA GCAGCCGCAC 
AACGGCTCCA CCTTCGCCGG CGCCACCGCT CTGCTCCACA TAAAGACGGA GCAAAATACG 
CTGCTCACTC CGCTCCAGCT GCAGCAGCAG CAACAACAGC AGCAGGGTCT ACACGGTGCG 
GCAGGCAACG GAGGCAGCAG CAACGGCAAC AATGCCCACC AGCAGCAACA GCCGCTGGCC 
ATCCCACAAC GCCCGCTGCT CCACAATCTG CTTAGCGGCG GTGCCATCCA CAATCCGCAC 
CACAGAAACT ACACGACGGC CACAACAGGT TCATTTCCGC CCAGTCCCGC GGACAGCGGC 
GTCTCCGACG TGGACAGCTC CAGTTCCGGC GGCCAGCCCT GCGCCGACGA GCTGAAGGCG 
CGTCTCGGGA TGCCACCAGC CACCTCGGCG TCGGCAGCGG CCGCGGCAGC AGCGGCGGCG 
GCGGCAGCGG CACACCTGCA CACGGGCACC TTTCTGCATC CGAATTTATA CCAGAACAAT 
GCGGCAAATT CGCTGCGGAA CATATGGAAT CGCAGTGTTG GGGTGCCCGA CAACTACTAC 
GGAAGCAGTG GCGCCGGCAG TGGGGGCACC CAGCCAGGTG GTCCGGGAAA CCCCCAAACG 
CCTGGCTACC TGACAACGTC CTACTTCAAC GCACCGACAG CGGCAACGGC AGCGGCGTCC 
CAGCGAGGAA CCACAATCAA TGGCTATCAT TCCCTGCACC AGCAGCAGCA GCAGCAACAG 
CAGTCGCAGC AATCGCAGCA GCAACAGCAA CTGGCCCACC AGCAACTGTC GCACCAGCAG 
CAGCAGGCGT TGCATCAGCA ACTGTCGCAC CAACAGCAGC AGCAGCAACA GCAACAGCAG 
CAACACCCGC ACTCCCAACT GAACGGTCCG CATCCGCACT CACATCCGCA CTCTCATCCG 
CATTCGCATC CGCACGCGGG CCAGCACACG CATTCCACAA TCGCTGCAGC GGCGGCAGCG 
GCAGCAGCAT CTGTCGTCAG CAGCAGCAGC AGCGCCGTTG CAGCGGCAGC CATGCTGAGC 
GCCAGTGCAG CGGCAGCAGC AACGGCGGCA GCTGCGGCGG GCGGATCCCA GAGTGTTATC 
CAACCGGCGA CGTCCAGCGT CAGCTACGAT CTCTCCTACA TGCTGGAGCT GGGCGGATTC 
CAGCAGCGGA AGGCGAAGAA GCCGCGCAAA CCGAAGCTGG AGATGGGCGT AAAGCGGCGC 
AGCCGGGAGG GATCCACCAC CTATCTGTGG GAGTTCCTCC TCAAACTGCT CCAGGATCGC 
GAATACTGTC CGCGTTTCAT CAAGTGGACG AACCGGGAGA AGGGCGTCTT CAAGCTGGTC 
GACTCGAAGG CTGTGTCCCG CCTGTGGGGC ATGCACAAGA ACAAGCCGGA CATGAACTAC 
GAGACGATGG GCAGGGCGCT GAGGTACTAC TACCAGCGCG GCATACTGGC CAAGGTGGAT 
GGCCAGCGGC TGGTCTACCA GTTCGTGGAT GTGCCCAAGG ACATCATCGA GATTGACTGC 
AACGGTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip74EF ( NM_001014590.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014590.1 Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21021
Clone ID Related Accession (Same CDS sequence)	NM_168741.2 , NM_001014590.2
Accession Version	NM_001014590.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 74EF, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014590.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATGG TGCAACATTT GGTGGCCGCC TCGGCGCACA ACTTCGCCAG CCAGGCGGCG 
GCATCGCTTG TCAATGTGTC CAGCTCCAGC TCTAGTTCCA GTTCGAGTTC CTCGTCCTCG 
CTGTCGCTGT CTTCATCCTC ATCCAGCTCC TCGCTGTCCT CGGCCACGCC CACGCCGGTG 
GCCTCGCCCG TGACGCCCAC ATCTCCACCG CCGGCAGCCG CCGCACCCGC AGAAGCCTCG 
CCGCCAGCAG GAGCTGAGTT GCAGGAGGAT GGGCAGCAGG CTAAGACTCA GGAGGATCCT 
ACTATGAAGG ACCAAGACAT GCTGGAAAAG ACGAGGCAAG AGGTGAAGGA TCCAGTTAAT 
GTGGAGGAAC CAGGTGCCAT AGTTGATACC GAGTCAGTGA TGGCGCGGCA AAGTCCAAGT 
CCAGTTGCGT CCACCAAAGT CCCCGAGTCA CTAGAAGAGA TTTCCAACAA ATCGCCGCCC 
GTTCAAGAGG ATGAAGAGGA GTCTGAATCC GTAGCCTCCG ACTGTCGCGA GTTCAAAGTG 
CTCTATAATC ACCTAAGGCA GCAGCAACAC CACCACTCTC CCTCCTCGCC AGACAAGACG 
CGCAGCACCC TGGACGATGT GTCCAAGATC CTCTGGGAGC GAAAGCAGCA GCTGCAGCGC 
AGCTCAGTGA TTACCGCAGC ACCCACTCTG CAGCCGCAGC AGCACCAGCA GCCCATGAGC 
GACATCGAGG ACGAGGAGAC GCTGGAGGAT GTCGATGACG CGGATGCGGA CGTGGAGGCA 
GACGCCGAGG ACGAGGAGCT ACTGGAGCAG TACCAAAACG GCTACGACTC GCCACTGGAT 
CTGTCCCTGG GTGGAGCCAC CAGTGCAGCC GCATCAGCTG CCGCTGCCGC CTCGGCGGTC 
AGTCGCCGAC GGGGACGCAC CTACTCCGGC ACGGAATCCG ACGACTCCGC GCAGTGCGAA 
CGGGCGAGGA TGCGACTCAA GCCGGAACGC AAGGCGGAGA GATCGGCGGC CTACAAGAAG 
AGCTTGATGA AGCGATATTA CACCGAAATT CCTATTGTCA AGCAATCGAC AAGTCCGGCA 
CCGCAGCAGC AACTGCAGCA GCAGCACCAC CTGCAACAGC AGCAACAGCA GCAGCCGCAC 
AACGGCTCCA CCTTCGCCGG CGCCACCGCT CTGCTCCACA TAAAGACGGA GCAAAATACG 
CTGCTCACTC CGCTCCAGCT GCAGCAGCAG CAACAACAGC AGCAGGGTCT ACACGGTGCG 
GCAGGCAACG GAGGCAGCAG CAACGGCAAC AATGCCCACC AGCAGCAACA GCCGCTGGCC 
ATCCCACAAC GCCCGCTGCT CCACAATCTG CTTAGCGGCG GTGCCATCCA CAATCCGCAC 
CACAGAAACT ACACGACGGC CACAACAGGT TCATTTCCGC CCAGTCCCGC GGACAGCGGC 
GTCTCCGACG TGGACAGCTC CAGTTCCGGC GGCCAGCCCT GCGCCGACGA GCTGAAGGCG 
CGTCTCGGGA TGCCACCAGC CACCTCGGCG TCGGCAGCGG CCGCGGCAGC AGCGGCGGCG 
GCGGCAGCGG CACACCTGCA CACGGGCACC TTTCTGCATC CGAATTTATA CCAGAACAAT 
GCGGCAAATT CGCTGCGGAA CATATGGAAT CGCAGTGTTG GGGTGCCCGA CAACTACTAC 
GGAAGCAGTG GCGCCGGCAG TGGGGGCACC CAGCCAGGTG GTCCGGGAAA CCCCCAAACG 
CCTGGCTACC TGACAACGTC CTACTTCAAC GCACCGACAG CGGCAACGGC AGCGGCGTCC 
CAGCGAGGAA CCACAATCAA TGGCTATCAT TCCCTGCACC AGCAGCAGCA GCAGCAACAG 
CAGTCGCAGC AATCGCAGCA GCAACAGCAA CTGGCCCACC AGCAACTGTC GCACCAGCAG 
CAGCAGGCGT TGCATCAGCA ACTGTCGCAC CAACAGCAGC AGCAGCAACA GCAACAGCAG 
CAACACCCGC ACTCCCAACT GAACGGTCCG CATCCGCACT CACATCCGCA CTCTCATCCG 
CATTCGCATC CGCACGCGGG CCAGCACACG CATTCCACAA TCGCTGCAGC GGCGGCAGCG 
GCAGCAGCAT CTGTCGTCAG CAGCAGCAGC AGCGCCGTTG CAGCGGCAGC CATGCTGAGC 
GCCAGTGCAG CGGCAGCAGC AACGGCGGCA GCTGCGGCGG GCGGATCCCA GAGTGTTATC 
CAACCGGCGA CGTCCAGCGT CAGCTACGAT CTCTCCTACA TGCTGGAGCT GGGCGGATTC 
CAGCAGCGGA AGGCGAAGAA GCCGCGCAAA CCGAAGCTGG AGATGGGCGT AAAGCGGCGC 
AGCCGGGAGG GATCCACCAC CTATCTGTGG GAGTTCCTCC TCAAACTGCT CCAGGATCGC 
GAATACTGTC CGCGTTTCAT CAAGTGGACG AACCGGGAGA AGGGCGTCTT CAAGCTGGTC 
GACTCGAAGG CTGTGTCCCG CCTGTGGGGC ATGCACAAGA ACAAGCCGGA CATGAACTAC 
GAGACGATGG GCAGGGCGCT GAGGTACTAC TACCAGCGCG GCATACTGGC CAAGGTGGAT 
GGCCAGCGGC TGGTCTACCA GTTCGTGGAT GTGCCCAAGG ACATCATCGA GATTGACTGC 
AACGGTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014590.1
CDS	490..3141
Translation	MIMVQHLVAASAHNFASQAAASLVNVSSSSSSSSSSSSSSLSLSSSSSSSSLSSATPTPVASPVTPTSPPPAAAAPAEASPPAGAELQEDGQQAKTQEDPTMKDQDMLEKTRQEVKDPVNVEEPGAIVDTESVMARQSPSPVASTKVPESLEEISNKSPPVQEDEEESESVASDCREFKVLYNHLRQQQHHHSPSSPDKTRSTLDDVSKILWERKQQLQRSSVITAAPTLQPQQHQQPMSDIEDEETLEDVDDADADVEADAEDEELLEQYQNGYDSPLDLSLGGATSAAASAAAAASAVSRRRGRTYSGTESDDSAQCERARMRLKPERKAERSAAYKKSLMKRYYTEIPIVKQSTSPAPQQQLQQQHHLQQQQQQQPHNGSTFAGATALLHIKTEQNTLLTPLQLQQQQQQQQGLHGAAGNGGSSNGNNAHQQQQPLAIPQRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPPATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGAGSGGTQPGGPGNPQTPGYLTTSYFNAPTAATAAASQRGTTINGYHSLHQQQQQQQQSQQSQQQQQLAHQQLSHQQQQALHQQLSHQQQQQQQQQQQHPHSQLNGPHPHSHPHSHPHSHPHAGQHTHSTIAAAAAAAAASVVSSSSSAVAAAAMLSASAAAAATAAAAAGGSQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIIEIDCNGV

Target ORF information:

RefSeq Version	NM_001014590.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014590.2

ORF Insert Sequence:

ATGATTATGG TGCAACATTT GGTGGCCGCC TCGGCGCACA ACTTCGCCAG CCAGGCGGCG 
GCATCGCTTG TCAATGTGTC CAGCTCCAGC TCTAGTTCCA GTTCGAGTTC CTCGTCCTCG 
CTGTCGCTGT CTTCATCCTC ATCCAGCTCC TCGCTGTCCT CGGCCACGCC CACGCCGGTG 
GCCTCGCCCG TGACGCCCAC ATCTCCACCG CCGGCAGCCG CCGCACCCGC AGAAGCCTCG 
CCGCCAGCAG GAGCTGAGTT GCAGGAGGAT GGGCAGCAGG CTAAGACTCA GGAGGATCCT 
ACTATGAAGG ACCAAGACAT GCTGGAAAAG ACGAGGCAAG AGGTGAAGGA TCCAGTTAAT 
GTGGAGGAAC CAGGTGCCAT AGTTGATACC GAGTCAGTGA TGGCGCGGCA AAGTCCAAGT 
CCAGTTGCGT CCACCAAAGT CCCCGAGTCA CTAGAAGAGA TTTCCAACAA ATCGCCGCCC 
GTTCAAGAGG ATGAAGAGGA GTCTGAATCC GTAGCCTCCG ACTGTCGCGA GTTCAAAGTG 
CTCTATAATC ACCTAAGGCA GCAGCAACAC CACCACTCTC CCTCCTCGCC AGACAAGACG 
CGCAGCACCC TGGACGATGT GTCCAAGATC CTCTGGGAGC GAAAGCAGCA GCTGCAGCGC 
AGCTCAGTGA TTACCGCAGC ACCCACTCTG CAGCCGCAGC AGCACCAGCA GCCCATGAGC 
GACATCGAGG ACGAGGAGAC GCTGGAGGAT GTCGATGACG CGGATGCGGA CGTGGAGGCA 
GACGCCGAGG ACGAGGAGCT ACTGGAGCAG TACCAAAACG GCTACGACTC GCCACTGGAT 
CTGTCCCTGG GTGGAGCCAC CAGTGCAGCC GCATCAGCTG CCGCTGCCGC CTCGGCGGTC 
AGTCGCCGAC GGGGACGCAC CTACTCCGGC ACGGAATCCG ACGACTCCGC GCAGTGCGAA 
CGGGCGAGGA TGCGACTCAA GCCGGAACGC AAGGCGGAGA GATCGGCGGC CTACAAGAAG 
AGCTTGATGA AGCGATATTA CACCGAAATT CCTATTGTCA AGCAATCGAC AAGTCCGGCA 
CCGCAGCAGC AACTGCAGCA GCAGCACCAC CTGCAACAGC AGCAACAGCA GCAGCCGCAC 
AACGGCTCCA CCTTCGCCGG CGCCACCGCT CTGCTCCACA TAAAGACGGA GCAAAATACG 
CTGCTCACTC CGCTCCAGCT GCAGCAGCAG CAACAACAGC AGCAGGGTCT ACACGGTGCG 
GCAGGCAACG GAGGCAGCAG CAACGGCAAC AATGCCCACC AGCAGCAACA GCCGCTGGCC 
ATCCCACAAC GCCCGCTGCT CCACAATCTG CTTAGCGGCG GTGCCATCCA CAATCCGCAC 
CACAGAAACT ACACGACGGC CACAACAGGT TCATTTCCGC CCAGTCCCGC GGACAGCGGC 
GTCTCCGACG TGGACAGCTC CAGTTCCGGC GGCCAGCCCT GCGCCGACGA GCTGAAGGCG 
CGTCTCGGGA TGCCACCAGC CACCTCGGCG TCGGCAGCGG CCGCGGCAGC AGCGGCGGCG 
GCGGCAGCGG CACACCTGCA CACGGGCACC TTTCTGCATC CGAATTTATA CCAGAACAAT 
GCGGCAAATT CGCTGCGGAA CATATGGAAT CGCAGTGTTG GGGTGCCCGA CAACTACTAC 
GGAAGCAGTG GCGCCGGCAG TGGGGGCACC CAGCCAGGTG GTCCGGGAAA CCCCCAAACG 
CCTGGCTACC TGACAACGTC CTACTTCAAC GCACCGACAG CGGCAACGGC AGCGGCGTCC 
CAGCGAGGAA CCACAATCAA TGGCTATCAT TCCCTGCACC AGCAGCAGCA GCAGCAACAG 
CAGTCGCAGC AATCGCAGCA GCAACAGCAA CTGGCCCACC AGCAACTGTC GCACCAGCAG 
CAGCAGGCGT TGCATCAGCA ACTGTCGCAC CAACAGCAGC AGCAGCAACA GCAACAGCAG 
CAACACCCGC ACTCCCAACT GAACGGTCCG CATCCGCACT CACATCCGCA CTCTCATCCG 
CATTCGCATC CGCACGCGGG CCAGCACACG CATTCCACAA TCGCTGCAGC GGCGGCAGCG 
GCAGCAGCAT CTGTCGTCAG CAGCAGCAGC AGCGCCGTTG CAGCGGCAGC CATGCTGAGC 
GCCAGTGCAG CGGCAGCAGC AACGGCGGCA GCTGCGGCGG GCGGATCCCA GAGTGTTATC 
CAACCGGCGA CGTCCAGCGT CAGCTACGAT CTCTCCTACA TGCTGGAGCT GGGCGGATTC 
CAGCAGCGGA AGGCGAAGAA GCCGCGCAAA CCGAAGCTGG AGATGGGCGT AAAGCGGCGC 
AGCCGGGAGG GATCCACCAC CTATCTGTGG GAGTTCCTCC TCAAACTGCT CCAGGATCGC 
GAATACTGTC CGCGTTTCAT CAAGTGGACG AACCGGGAGA AGGGCGTCTT CAAGCTGGTC 
GACTCGAAGG CTGTGTCCCG CCTGTGGGGC ATGCACAAGA ACAAGCCGGA CATGAACTAC 
GAGACGATGG GCAGGGCGCT GAGGTACTAC TACCAGCGCG GCATACTGGC CAAGGTGGAT 
GGCCAGCGGC TGGTCTACCA GTTCGTGGAT GTGCCCAAGG ACATCATCGA GATTGACTGC 
AACGGTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip74EF ( NM_168740.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730287.1 Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa21022
Clone ID Related Accession (Same CDS sequence)	NM_168740.3
Accession Version	NM_168740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 74EF, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168740.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCTTTA TCGACGATGC ACTGCTCTGG TGTCCAGATA ACGATGGTCG TCTTGTTGGA 
GGTCTAGACT TGGGAACATG CATAGCCGAT GATTCTACGG CTAACGGAAC GGAAAACTTG 
AACCCCTCGA TCCAATCAGC TGGCAATCCG AACAACCCGC AGCAGAGCGT CGGTGGCGAG 
ATCCTGGGAT CCGTGGAGTC CGCCGGCAAC GAACTGAATG GAGCGGCGGC CCGCAACGTG 
AACGTGGTGG TGGAACCGCT CTGCGGCGGC GACTCCTCCG ACGAGCTGTT CCGCAGCTTC 
TCCGAGAGCA ACTTCGAGAT CGAGAGCCTG CTGTCCGATT TGGCCACCGT CGAGGTGAAA 
GTCGAAAATG AGGAGAACAA CAACAATGTC ATCACCGATG ATGACTTCGC CAGCGTCGCT 
GCCGCCGTGG TGGCCAACGA TGATCTGCTA GCCAAAGAGA ATGCCCAGCT GAGTGCCCAG 
GGTCTGGTCG ATTCGGTGGC CGCCAGTCTG GCCGATTCCG GCGATGCCGG TGGTCAGCAG 
GCGCTACTGG CCTTCGGCTC TTCCAGCTCG GCAGCCTCGG CGATTGCAGC AGCAGCTGCC 
GCCCTCTGTG GCGATTTGAT CAACAACAAC AACAACAACA GCAACAGCAA CAACAACAGC 
AATGGCAATG GCAATCACGG CGGCGGGGGA GGAGGAGCTA GTTCCGGCGG CGGAGTAGCC 
GGCGACTGTG CCACCAAGCT GGAGTACGCC CTCATGGGCG GACAGCCGCT GGCGGAGGAG 
CCGCGCTTCG TGACCAGCGC CGCCGCCAAT CCCCTTCTCG TCGAGAAGCT AATGTCCAAG 
TGCCTGAACA TTGAGAAGCG AATGGACAAG CTCAGCGACA CCGAAATTCC TATTGTCAAG 
CAATCGACAA GTCCGGCACC GCAGCAGCAA CTGCAGCAGC AGCACCACCT GCAACAGCAG 
CAACAGCAGC AGCCGCACAA CGGCTCCACC TTCGCCGGCG CCACCGCTCT GCTCCACATA 
AAGACGGAGC AAAATACGCT GCTCACTCCG CTCCAGCTGC AGCAGCAGCA ACAACAGCAG 
CAGGGTCTAC ACGGTGCGGC AGGCAACGGA GGCAGCAGCA ACGGCAACAA TGCCCACCAG 
CAGCAACAGC CGCTGGCCAT CCCACAACGC CCGCTGCTCC ACAATCTGCT TAGCGGCGGT 
GCCATCCACA ATCCGCACCA CAGAAACTAC ACGACGGCCA CAACAGGTTC ATTTCCGCCC 
AGTCCCGCGG ACAGCGGCGT CTCCGACGTG GACAGCTCCA GTTCCGGCGG CCAGCCCTGC 
GCCGACGAGC TGAAGGCGCG TCTCGGGATG CCACCAGCCA CCTCGGCGTC GGCAGCGGCC 
GCGGCAGCAG CGGCGGCGGC GGCAGCGGCA CACCTGCACA CGGGCACCTT TCTGCATCCG 
AATTTATACC AGAACAATGC GGCAAATTCG CTGCGGAACA TATGGAATCG CAGTGTTGGG 
GTGCCCGACA ACTACTACGG AAGCAGTGGC GCCGGCAGTG GGGGCACCCA GCCAGGTGGT 
CCGGGAAACC CCCAAACGCC TGGCTACCTG ACAACGTCCT ACTTCAACGC ACCGACAGCG 
GCAACGGCAG CGGCGTCCCA GCGAGGAACC ACAATCAATG GCTATCATTC CCTGCACCAG 
CAGCAGCAGC AGCAACAGCA GTCGCAGCAA TCGCAGCAGC AACAGCAACT GGCCCACCAG 
CAACTGTCGC ACCAGCAGCA GCAGGCGTTG CATCAGCAAC TGTCGCACCA ACAGCAGCAG 
CAGCAACAGC AACAGCAGCA ACACCCGCAC TCCCAACTGA ACGGTCCGCA TCCGCACTCA 
CATCCGCACT CTCATCCGCA TTCGCATCCG CACGCGGGCC AGCACACGCA TTCCACAATC 
GCTGCAGCGG CGGCAGCGGC AGCAGCATCT GTCGTCAGCA GCAGCAGCAG CGCCGTTGCA 
GCGGCAGCCA TGCTGAGCGC CAGTGCAGCG GCAGCAGCAA CGGCGGCAGC TGCGGCGGGC 
GGATCCCAGA GTGTTATCCA ACCGGCGACG TCCAGCGTCA GCTACGATCT CTCCTACATG 
CTGGAGCTGG GCGGATTCCA GCAGCGGAAG GCGAAGAAGC CGCGCAAACC GAAGCTGGAG 
ATGGGCGTAA AGCGGCGCAG CCGGGAGGGA TCCACCACCT ATCTGTGGGA GTTCCTCCTC 
AAACTGCTCC AGGATCGCGA ATACTGTCCG CGTTTCATCA AGTGGACGAA CCGGGAGAAG 
GGCGTCTTCA AGCTGGTCGA CTCGAAGGCT GTGTCCCGCC TGTGGGGCAT GCACAAGAAC 
AAGCCGGACA TGAACTACGA GACGATGGGC AGGGCGCTGA GGTACTACTA CCAGCGCGGC 
ATACTGGCCA AGGTGGATGG CCAGCGGCTG GTCTACCAGT TCGTGGATGT GCCCAAGGAC 
ATCATCGAGA TTGACTGCAA CGGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730287.1
CDS	1911..4400
Translation	MPFIDDALLWCPDNDGRLVGGLDLGTCIADDSTANGTENLNPSIQSAGNPNNPQQSVGGEILGSVESAGNELNGAAARNVNVVVEPLCGGDSSDELFRSFSESNFEIESLLSDLATVEVKVENEENNNNVITDDDFASVAAAVVANDDLLAKENAQLSAQGLVDSVAASLADSGDAGGQQALLAFGSSSSAASAIAAAAAALCGDLINNNNNNSNSNNNSNGNGNHGGGGGGASSGGGVAGDCATKLEYALMGGQPLAEEPRFVTSAAANPLLVEKLMSKCLNIEKRMDKLSDTEIPIVKQSTSPAPQQQLQQQHHLQQQQQQQPHNGSTFAGATALLHIKTEQNTLLTPLQLQQQQQQQQGLHGAAGNGGSSNGNNAHQQQQPLAIPQRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPPATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGAGSGGTQPGGPGNPQTPGYLTTSYFNAPTAATAAASQRGTTINGYHSLHQQQQQQQQSQQSQQQQQLAHQQLSHQQQQALHQQLSHQQQQQQQQQQQHPHSQLNGPHPHSHPHSHPHSHPHAGQHTHSTIAAAAAAAAASVVSSSSSAVAAAAMLSASAAAAATAAAAAGGSQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIIEIDCNGV

Target ORF information:

RefSeq Version	NM_168740.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168740.3

ORF Insert Sequence:

ATGCCCTTTA TCGACGATGC ACTGCTCTGG TGTCCAGATA ACGATGGTCG TCTTGTTGGA 
GGTCTAGACT TGGGAACATG CATAGCCGAT GATTCTACGG CTAACGGAAC GGAAAACTTG 
AACCCCTCGA TCCAATCAGC TGGCAATCCG AACAACCCGC AGCAGAGCGT CGGTGGCGAG 
ATCCTGGGAT CCGTGGAGTC CGCCGGCAAC GAACTGAATG GAGCGGCGGC CCGCAACGTG 
AACGTGGTGG TGGAACCGCT CTGCGGCGGC GACTCCTCCG ACGAGCTGTT CCGCAGCTTC 
TCCGAGAGCA ACTTCGAGAT CGAGAGCCTG CTGTCCGATT TGGCCACCGT CGAGGTGAAA 
GTCGAAAATG AGGAGAACAA CAACAATGTC ATCACCGATG ATGACTTCGC CAGCGTCGCT 
GCCGCCGTGG TGGCCAACGA TGATCTGCTA GCCAAAGAGA ATGCCCAGCT GAGTGCCCAG 
GGTCTGGTCG ATTCGGTGGC CGCCAGTCTG GCCGATTCCG GCGATGCCGG TGGTCAGCAG 
GCGCTACTGG CCTTCGGCTC TTCCAGCTCG GCAGCCTCGG CGATTGCAGC AGCAGCTGCC 
GCCCTCTGTG GCGATTTGAT CAACAACAAC AACAACAACA GCAACAGCAA CAACAACAGC 
AATGGCAATG GCAATCACGG CGGCGGGGGA GGAGGAGCTA GTTCCGGCGG CGGAGTAGCC 
GGCGACTGTG CCACCAAGCT GGAGTACGCC CTCATGGGCG GACAGCCGCT GGCGGAGGAG 
CCGCGCTTCG TGACCAGCGC CGCCGCCAAT CCCCTTCTCG TCGAGAAGCT AATGTCCAAG 
TGCCTGAACA TTGAGAAGCG AATGGACAAG CTCAGCGACA CCGAAATTCC TATTGTCAAG 
CAATCGACAA GTCCGGCACC GCAGCAGCAA CTGCAGCAGC AGCACCACCT GCAACAGCAG 
CAACAGCAGC AGCCGCACAA CGGCTCCACC TTCGCCGGCG CCACCGCTCT GCTCCACATA 
AAGACGGAGC AAAATACGCT GCTCACTCCG CTCCAGCTGC AGCAGCAGCA ACAACAGCAG 
CAGGGTCTAC ACGGTGCGGC AGGCAACGGA GGCAGCAGCA ACGGCAACAA TGCCCACCAG 
CAGCAACAGC CGCTGGCCAT CCCACAACGC CCGCTGCTCC ACAATCTGCT TAGCGGCGGT 
GCCATCCACA ATCCGCACCA CAGAAACTAC ACGACGGCCA CAACAGGTTC ATTTCCGCCC 
AGTCCCGCGG ACAGCGGCGT CTCCGACGTG GACAGCTCCA GTTCCGGCGG CCAGCCCTGC 
GCCGACGAGC TGAAGGCGCG TCTCGGGATG CCACCAGCCA CCTCGGCGTC GGCAGCGGCC 
GCGGCAGCAG CGGCGGCGGC GGCAGCGGCA CACCTGCACA CGGGCACCTT TCTGCATCCG 
AATTTATACC AGAACAATGC GGCAAATTCG CTGCGGAACA TATGGAATCG CAGTGTTGGG 
GTGCCCGACA ACTACTACGG AAGCAGTGGC GCCGGCAGTG GGGGCACCCA GCCAGGTGGT 
CCGGGAAACC CCCAAACGCC TGGCTACCTG ACAACGTCCT ACTTCAACGC ACCGACAGCG 
GCAACGGCAG CGGCGTCCCA GCGAGGAACC ACAATCAATG GCTATCATTC CCTGCACCAG 
CAGCAGCAGC AGCAACAGCA GTCGCAGCAA TCGCAGCAGC AACAGCAACT GGCCCACCAG 
CAACTGTCGC ACCAGCAGCA GCAGGCGTTG CATCAGCAAC TGTCGCACCA ACAGCAGCAG 
CAGCAACAGC AACAGCAGCA ACACCCGCAC TCCCAACTGA ACGGTCCGCA TCCGCACTCA 
CATCCGCACT CTCATCCGCA TTCGCATCCG CACGCGGGCC AGCACACGCA TTCCACAATC 
GCTGCAGCGG CGGCAGCGGC AGCAGCATCT GTCGTCAGCA GCAGCAGCAG CGCCGTTGCA 
GCGGCAGCCA TGCTGAGCGC CAGTGCAGCG GCAGCAGCAA CGGCGGCAGC TGCGGCGGGC 
GGATCCCAGA GTGTTATCCA ACCGGCGACG TCCAGCGTCA GCTACGATCT CTCCTACATG 
CTGGAGCTGG GCGGATTCCA GCAGCGGAAG GCGAAGAAGC CGCGCAAACC GAAGCTGGAG 
ATGGGCGTAA AGCGGCGCAG CCGGGAGGGA TCCACCACCT ATCTGTGGGA GTTCCTCCTC 
AAACTGCTCC AGGATCGCGA ATACTGTCCG CGTTTCATCA AGTGGACGAA CCGGGAGAAG 
GGCGTCTTCA AGCTGGTCGA CTCGAAGGCT GTGTCCCGCC TGTGGGGCAT GCACAAGAAC 
AAGCCGGACA TGAACTACGA GACGATGGGC AGGGCGCTGA GGTACTACTA CCAGCGCGGC 
ATACTGGCCA AGGTGGATGG CCAGCGGCTG GTCTACCAGT TCGTGGATGT GCCCAAGGAC 
ATCATCGAGA TTGACTGCAA CGGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip74EF ( NM_168739.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730286.3 Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant C, mRNA.

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CloneID	OFa21023
Clone ID Related Accession (Same CDS sequence)	NM_168739.3
Accession Version	NM_168739.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 74EF, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168739.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

CTGGACCTGG GTGGCATCAA GATTGAGCAG AACTTTCGCG ACTGGCAGGG GTATTCAGGA 
AACGGCTACA CGCTGGCAGT TAACAGTGTT TGCAGTGCAG GCTTTGCCCT ATCAGCGATG 
CCCTTTATCG ACGATGCACT GCTCTGGTGT CCAGATAACG ATGGTCGTCT TGTTGGAGGT 
CTAGACTTGG GAACATGCAT AGCCGATGAT TCTACGGCTA ACGGAACGGA AAACTTGAAC 
CCCTCGATCC AATCAGCTGG CAATCCGAAC AACCCGCAGC AGAGCGTCGG TGGCGAGATC 
CTGGGATCCG TGGAGTCCGC CGGCAACGAA CTGAATGGAG CGGCGGCCCG CAACGTGAAC 
GTGGTGGTGG AACCGCTCTG CGGCGGCGAC TCCTCCGACG AGCTGTTCCG CAGCTTCTCC 
GAGAGCAACT TCGAGATCGA GAGCCTGCTG TCCGATTTGG CCACCGTCGA GGTGAAAGTC 
GAAAATGAGG AGAACAACAA CAATGTCATC ACCGATGATG ACTTCGCCAG CGTCGCTGCC 
GCCGTGGTGG CCAACGATGA TCTGCTAGCC AAAGAGAATG CCCAGCTGAG TGCCCAGGGT 
CTGGTCGATT CGGTGGCCGC CAGTCTGGCC GATTCCGGCG ATGCCGGTGG TCAGCAGGCG 
CTACTGGCCT TCGGCTCTTC CAGCTCGGCA GCCTCGGCGA TTGCAGCAGC AGCTGCCGCC 
CTCTGTGGCG ATTTGATCAA CAACAACAAC AACAACAGCA ACAGCAACAA CAACAGCAAT 
GGCAATGGCA ATCACGGCGG CGGGGGAGGA GGAGCTAGTT CCGGCGGCGG AGTAGCCGGC 
GACTGTGCCA CCAAGCTGGA GTACGCCCTC ATGGGCGGAC AGCCGCTGGC GGAGGAGCCG 
CGCTTCGTGA CCAGCGCCGC CGCCAATCCC CTTCTCGTCG AGAAGCTAAT GTCCAAGTGC 
CTGAACATTG AGAAGCGAAT GGACAAGCTC AGCGACACCG AAATTCCTAT TGTCAAGCAA 
TCGACAAGTC CGGCACCGCA GCAGCAACTG CAGCAGCAGC ACCACCTGCA ACAGCAGCAA 
CAGCAGCAGC CGCACAACGG CTCCACCTTC GCCGGCGCCA CCGCTCTGCT CCACATAAAG 
ACGGAGCAAA ATACGCTGCT CACTCCGCTC CAGCTGCAGC AGCAGCAACA ACAGCAGCAG 
GGTCTACACG GTGCGGCAGG CAACGGAGGC AGCAGCAACG GCAACAATGC CCACCAGCAG 
CAACAGCCGC TGGCCATCCC ACAACGCCCG CTGCTCCACA ATCTGCTTAG CGGCGGTGCC 
ATCCACAATC CGCACCACAG AAACTACACG ACGGCCACAA CAGGTTCATT TCCGCCCAGT 
CCCGCGGACA GCGGCGTCTC CGACGTGGAC AGCTCCAGTT CCGGCGGCCA GCCCTGCGCC 
GACGAGCTGA AGGCGCGTCT CGGGATGCCA CCAGCCACCT CGGCGTCGGC AGCGGCCGCG 
GCAGCAGCGG CGGCGGCGGC AGCGGCACAC CTGCACACGG GCACCTTTCT GCATCCGAAT 
TTATACCAGA ACAATGCGGC AAATTCGCTG CGGAACATAT GGAATCGCAG TGTTGGGGTG 
CCCGACAACT ACTACGGAAG CAGTGGCGCC GGCAGTGGGG GCACCCAGCC AGGTGGTCCG 
GGAAACCCCC AAACGCCTGG CTACCTGACA ACGTCCTACT TCAACGCACC GACAGCGGCA 
ACGGCAGCGG CGTCCCAGCG AGGAACCACA ATCAATGGCT ATCATTCCCT GCACCAGCAG 
CAGCAGCAGC AACAGCAGTC GCAGCAATCG CAGCAGCAAC AGCAACTGGC CCACCAGCAA 
CTGTCGCACC AGCAGCAGCA GGCGTTGCAT CAGCAACTGT CGCACCAACA GCAGCAGCAG 
CAACAGCAAC AGCAGCAACA CCCGCACTCC CAACTGAACG GTCCGCATCC GCACTCACAT 
CCGCACTCTC ATCCGCATTC GCATCCGCAC GCGGGCCAGC ACACGCATTC CACAATCGCT 
GCAGCGGCGG CAGCGGCAGC AGCATCTGTC GTCAGCAGCA GCAGCAGCGC CGTTGCAGCG 
GCAGCCATGC TGAGCGCCAG TGCAGCGGCA GCAGCAACGG CGGCAGCTGC GGCGGGCGGA 
TCCCAGAGTG TTATCCAACC GGCGACGTCC AGCGTCAGCT ACGATCTCTC CTACATGCTG 
GAGCTGGGCG GATTCCAGCA GCGGAAGGCG AAGAAGCCGC GCAAACCGAA GCTGGAGATG 
GGCGTAAAGC GGCGCAGCCG GGAGGGATCC ACCACCTATC TGTGGGAGTT CCTCCTCAAA 
CTGCTCCAGG ATCGCGAATA CTGTCCGCGT TTCATCAAGT GGACGAACCG GGAGAAGGGC 
GTCTTCAAGC TGGTCGACTC GAAGGCTGTG TCCCGCCTGT GGGGCATGCA CAAGAACAAG 
CCGGACATGA ACTACGAGAC GATGGGCAGG GCGCTGAGGT ACTACTACCA GCGCGGCATA 
CTGGCCAAGG TGGATGGCCA GCGGCTGGTC TACCAGTTCG TGGATGTGCC CAAGGACATC 
ATCGAGATTG ACTGCAACGG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730286.3
CDS	1794..4400
Translation	LDLGGIKIEQNFRDWQGYSGNGYTLAVNSVCSAGFALSAMPFIDDALLWCPDNDGRLVGGLDLGTCIADDSTANGTENLNPSIQSAGNPNNPQQSVGGEILGSVESAGNELNGAAARNVNVVVEPLCGGDSSDELFRSFSESNFEIESLLSDLATVEVKVENEENNNNVITDDDFASVAAAVVANDDLLAKENAQLSAQGLVDSVAASLADSGDAGGQQALLAFGSSSSAASAIAAAAAALCGDLINNNNNNSNSNNNSNGNGNHGGGGGGASSGGGVAGDCATKLEYALMGGQPLAEEPRFVTSAAANPLLVEKLMSKCLNIEKRMDKLSDTEIPIVKQSTSPAPQQQLQQQHHLQQQQQQQPHNGSTFAGATALLHIKTEQNTLLTPLQLQQQQQQQQGLHGAAGNGGSSNGNNAHQQQQPLAIPQRPLLHNLLSGGAIHNPHHRNYTTATTGSFPPSPADSGVSDVDSSSSGGQPCADELKARLGMPPATSASAAAAAAAAAAAAAHLHTGTFLHPNLYQNNAANSLRNIWNRSVGVPDNYYGSSGAGSGGTQPGGPGNPQTPGYLTTSYFNAPTAATAAASQRGTTINGYHSLHQQQQQQQQSQQSQQQQQLAHQQLSHQQQQALHQQLSHQQQQQQQQQQQHPHSQLNGPHPHSHPHSHPHSHPHAGQHTHSTIAAAAAAAAASVVSSSSSAVAAAAMLSASAAAAATAAAAAGGSQSVIQPATSSVSYDLSYMLELGGFQQRKAKKPRKPKLEMGVKRRSREGSTTYLWEFLLKLLQDREYCPRFIKWTNREKGVFKLVDSKAVSRLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIIEIDCNGV

Target ORF information:

RefSeq Version	NM_168739.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 74EF (Eip74EF), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168739.3

ORF Insert Sequence:

CTGGACCTGG GTGGCATCAA GATTGAGCAG AACTTTCGCG ACTGGCAGGG GTATTCAGGA 
AACGGCTACA CGCTGGCAGT TAACAGTGTT TGCAGTGCAG GCTTTGCCCT ATCAGCGATG 
CCCTTTATCG ACGATGCACT GCTCTGGTGT CCAGATAACG ATGGTCGTCT TGTTGGAGGT 
CTAGACTTGG GAACATGCAT AGCCGATGAT TCTACGGCTA ACGGAACGGA AAACTTGAAC 
CCCTCGATCC AATCAGCTGG CAATCCGAAC AACCCGCAGC AGAGCGTCGG TGGCGAGATC 
CTGGGATCCG TGGAGTCCGC CGGCAACGAA CTGAATGGAG CGGCGGCCCG CAACGTGAAC 
GTGGTGGTGG AACCGCTCTG CGGCGGCGAC TCCTCCGACG AGCTGTTCCG CAGCTTCTCC 
GAGAGCAACT TCGAGATCGA GAGCCTGCTG TCCGATTTGG CCACCGTCGA GGTGAAAGTC 
GAAAATGAGG AGAACAACAA CAATGTCATC ACCGATGATG ACTTCGCCAG CGTCGCTGCC 
GCCGTGGTGG CCAACGATGA TCTGCTAGCC AAAGAGAATG CCCAGCTGAG TGCCCAGGGT 
CTGGTCGATT CGGTGGCCGC CAGTCTGGCC GATTCCGGCG ATGCCGGTGG TCAGCAGGCG 
CTACTGGCCT TCGGCTCTTC CAGCTCGGCA GCCTCGGCGA TTGCAGCAGC AGCTGCCGCC 
CTCTGTGGCG ATTTGATCAA CAACAACAAC AACAACAGCA ACAGCAACAA CAACAGCAAT 
GGCAATGGCA ATCACGGCGG CGGGGGAGGA GGAGCTAGTT CCGGCGGCGG AGTAGCCGGC 
GACTGTGCCA CCAAGCTGGA GTACGCCCTC ATGGGCGGAC AGCCGCTGGC GGAGGAGCCG 
CGCTTCGTGA CCAGCGCCGC CGCCAATCCC CTTCTCGTCG AGAAGCTAAT GTCCAAGTGC 
CTGAACATTG AGAAGCGAAT GGACAAGCTC AGCGACACCG AAATTCCTAT TGTCAAGCAA 
TCGACAAGTC CGGCACCGCA GCAGCAACTG CAGCAGCAGC ACCACCTGCA ACAGCAGCAA 
CAGCAGCAGC CGCACAACGG CTCCACCTTC GCCGGCGCCA CCGCTCTGCT CCACATAAAG 
ACGGAGCAAA ATACGCTGCT CACTCCGCTC CAGCTGCAGC AGCAGCAACA ACAGCAGCAG 
GGTCTACACG GTGCGGCAGG CAACGGAGGC AGCAGCAACG GCAACAATGC CCACCAGCAG 
CAACAGCCGC TGGCCATCCC ACAACGCCCG CTGCTCCACA ATCTGCTTAG CGGCGGTGCC 
ATCCACAATC CGCACCACAG AAACTACACG ACGGCCACAA CAGGTTCATT TCCGCCCAGT 
CCCGCGGACA GCGGCGTCTC CGACGTGGAC AGCTCCAGTT CCGGCGGCCA GCCCTGCGCC 
GACGAGCTGA AGGCGCGTCT CGGGATGCCA CCAGCCACCT CGGCGTCGGC AGCGGCCGCG 
GCAGCAGCGG CGGCGGCGGC AGCGGCACAC CTGCACACGG GCACCTTTCT GCATCCGAAT 
TTATACCAGA ACAATGCGGC AAATTCGCTG CGGAACATAT GGAATCGCAG TGTTGGGGTG 
CCCGACAACT ACTACGGAAG CAGTGGCGCC GGCAGTGGGG GCACCCAGCC AGGTGGTCCG 
GGAAACCCCC AAACGCCTGG CTACCTGACA ACGTCCTACT TCAACGCACC GACAGCGGCA 
ACGGCAGCGG CGTCCCAGCG AGGAACCACA ATCAATGGCT ATCATTCCCT GCACCAGCAG 
CAGCAGCAGC AACAGCAGTC GCAGCAATCG CAGCAGCAAC AGCAACTGGC CCACCAGCAA 
CTGTCGCACC AGCAGCAGCA GGCGTTGCAT CAGCAACTGT CGCACCAACA GCAGCAGCAG 
CAACAGCAAC AGCAGCAACA CCCGCACTCC CAACTGAACG GTCCGCATCC GCACTCACAT 
CCGCACTCTC ATCCGCATTC GCATCCGCAC GCGGGCCAGC ACACGCATTC CACAATCGCT 
GCAGCGGCGG CAGCGGCAGC AGCATCTGTC GTCAGCAGCA GCAGCAGCGC CGTTGCAGCG 
GCAGCCATGC TGAGCGCCAG TGCAGCGGCA GCAGCAACGG CGGCAGCTGC GGCGGGCGGA 
TCCCAGAGTG TTATCCAACC GGCGACGTCC AGCGTCAGCT ACGATCTCTC CTACATGCTG 
GAGCTGGGCG GATTCCAGCA GCGGAAGGCG AAGAAGCCGC GCAAACCGAA GCTGGAGATG 
GGCGTAAAGC GGCGCAGCCG GGAGGGATCC ACCACCTATC TGTGGGAGTT CCTCCTCAAA 
CTGCTCCAGG ATCGCGAATA CTGTCCGCGT TTCATCAAGT GGACGAACCG GGAGAAGGGC 
GTCTTCAAGC TGGTCGACTC GAAGGCTGTG TCCCGCCTGT GGGGCATGCA CAAGAACAAG 
CCGGACATGA ACTACGAGAC GATGGGCAGG GCGCTGAGGT ACTACTACCA GCGCGGCATA 
CTGGCCAAGG TGGATGGCCA GCGGCTGGTC TACCAGTTCG TGGATGTGCC CAAGGACATC 
ATCGAGATTG ACTGCAACGG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eip74EF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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