Edc3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Edc3
Entrez Gene ID	39957
Full Name	Enhancer of decapping 3
Synonyms	CG6311,DmEDC3,Dmel\CG6311,EDC3,LSM16,LSm16
General protein information	Preferred Names Enhancer of decapping 3 Names CG6311 gene product from transcript CG6311-RB CG6311-PB Edc3-PB enhancer of decapping 3 enhancer of decapping-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 74D2-74D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_140735.3	NP_648992.1	enhancer of decapping 3

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Edc3	NP_648992.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003131	XP_312817.4
Homo sapiens (human)	EDC3	NP_001135915.1
Mus musculus (house mouse)	Edc3	NP_722494.1
Pan troglodytes (chimpanzee)	EDC3	XP_003952756.1
Bos taurus (cattle)	EDC3	NP_001026928.1
Xenopus tropicalis (tropical clawed frog)	edc3	NP_001120062.1
Rattus norvegicus (Norway rat)	Edc3	XP_002729954.2
Gallus gallus (chicken)	EDC3	NP_001025556.1
	EDC3	XP_002804909.1
Canis lupus familiaris (dog)	EDC3	XP_005638679.1
Danio rerio (zebrafish)	edc3	NP_001018136.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tral and EDC3 specifically interact with the decapping activator DCP1 and the DEAD-box helicase Me31B, in Drosophila cells.
Title: Similar modes of interaction enable Trailer Hitch and EDC3 to associate with DCP1 and Me31B in distinct protein complexes.

The modular architecture enables enhancer of decapping 3 (EDC3) to interact with multiple components of the decapping machinery, including DCP1, DCP2, and Me31B.
Title: A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body targeting.

The following Edc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21012	NM_140735.3 Latest version!	Drosophila melanogaster enhancer of decapping 3 (Edc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Edc3 ( NM_140735.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648992.1 Drosophila melanogaster enhancer of decapping 3 (Edc3), mRNA.

Price & Availability↑

CloneID	OFa21012
Clone ID Related Accession (Same CDS sequence)	NM_140735.3
Accession Version	NM_140735.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of decapping 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140735.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCCGA CGGATCAAGA CTGGATCGGC TGTGCAGTGT CTATAGCCTG CGACGAGGTC 
CTGGGCGTCT TCCAGGGCCT GATCAAGCAG ATTTCTGCCG AGGAGATCAC CATTGTGAGG 
GCTTTCCGCA ATGGCGTGCC GCTGCGAAAG CAGAACGCCG AGGTGGTCCT CAAGTGCACG 
GACATTCGCA GCATCGATCT CATCGAGCCC GCAAAGCAAG ATCTGGATGG GCACACGGCG 
CCGCCGCCAG TGGTCAACAA GCCCACGCCC GTCAAGCTGC CCCACTTCTC CAACATCCTG 
GGCAAGCAGC AGCAGCTCCA ACTCCAGCAG CAGCAGCAGC AGCTACAACT CCAGCAGCAA 
CAGAAACAGC AGTTCCAGGA GCAGGAACGA GAACAGGATC TGCCATCGAC GCCGCGGTCA 
AGGGCCAACG ACAATGGACG GGTAGCGGCT GGCGGAGCAT CGAGTTCTTC GGGCCCACGT 
GGCAACTTCA ATTCGGCGCT AAGCGATAAG ATGCATCAGC TGAAGCTAAT CGAGACCAAC 
GGCTCAAATG GCACCCTTAG AACACCACAA ACATCCCGTG CGTCCAGCGC TCAGCAGCAG 
CCGCAGATAT CAACGACTCC CAACAGCGTG GCCGCCTTTT TTGGCAACAT GATACCGCCG 
AAGGTCGAGG TGAAACTGGG CTCCTACGTG AGCAACACCC GGGAGAGTTA CTGCAGCAGC 
AGCGGAGATA GCGGCGAGGC AACTGGTTTG TCGTTGGGCA GCAGCAAACC CATAGATATC 
GTCAGCAACG GAGATGGATT TTACAAGCAG ACGGCAGCCT CGAGCTATGG GAATACAAAT 
GGAAATGTAA GAAGGAATGG CAATGCCAAT AACAATGGAA ATGGCACCGG AAATGGTTCT 
TATACCAATG GAAATGGCAA CGGCAACGGG AAGAACAAGC GGAACAGGGT GCGAAGGGAG 
AGCAGCATGC GGCAGCAACA GGTGCAACTG ACTTTTGGCA GCGAGGCGGA CGATCCGCTG 
ATCCACGAGG ATTTCGATTT TGAGGGCAAT CTAGCGCTGT TCGACAAGCA GGCAATTTGG 
GACGACATCG AGTCGACGAC CCAAAAACCA GATGTGGTGC GGCACATTGT GAACAATCAT 
CATCATAAGC CAGAGCAGAA GTATCGGCAC GACGAGAACA TCCTGGCCAG CAAACCACTA 
CAATTGCGCC AGATCGAAAG CATGTTCGGC GGCAGCCAGG ACTTTGTGAC GGACGACGGT 
CTCATTATAC CGACGATTCC GGCTTACGTT CGGAATAAAA TCGAGATAAG TGCCGATAAG 
GCGGGCCTCT CTCTGCAGAG GCAGATCGAC ATTTTGGCGC GCGGAGCCAG TGATCTGGCC 
ATTACACTGC TGGGCGGCGC CAGGCGATTG ACGCCGGCCA ACAACCATCA GTGGCCGAAG 
ATTGCCATCA TCTGCGATGG CGGCAAAAAT ATGAGAACAA TTAACATTGG AGCAGCCACA 
GGTCGCCAGC TGGCTTCGCA TGGGCTTACT GTCCTGCTGT ACGTGGAGCA GGCCAAACTG 
CTGGAGCAGA ACAGCAGCAG CCCAGAGATT TCATTGTTCA AGGCAACCGA CAATGTCATA 
GTTCACTCAG TAGACGCCTT ACCCACGCCA GATCTTGTGA TATTATCAAC TAACACCGCT 
AATTTATCGG ATGCGATCAG GAAATGGCTC AGTGTAAATC GCGCTTCGGT GCTTGCCATA 
GACCCGCCGC CGTGTGGCAT TAATGAGGTT GCTATCAAGT ACTCAATACT ACCCATACTA 
CCATTAAATG GCATATCAAC AGCAACAACA TCATCATCCT CTTCGGCTGC CGCCACACCA 
ACTCCCATTG CTAGCACTTC GGCAGCAGCA TCGGCTACCA AATCAGCAGC ATCAACGAAC 
AATTGTGGCA AATTGTATTT ATGTAATCTA GGTATACCGG ATAAATTCTA TCGTGATTGT 
GGCATTAAGT ACAAAAGTCC GTATGGACAT AAATATGTGA TACCGATTCA CTCAAAGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648992.1
CDS	394..2436
Translation	MGPTDQDWIGCAVSIACDEVLGVFQGLIKQISAEEITIVRAFRNGVPLRKQNAEVVLKCTDIRSIDLIEPAKQDLDGHTAPPPVVNKPTPVKLPHFSNILGKQQQLQLQQQQQQLQLQQQQKQQFQEQEREQDLPSTPRSRANDNGRVAAGGASSSSGPRGNFNSALSDKMHQLKLIETNGSNGTLRTPQTSRASSAQQQPQISTTPNSVAAFFGNMIPPKVEVKLGSYVSNTRESYCSSSGDSGEATGLSLGSSKPIDIVSNGDGFYKQTAASSYGNTNGNVRRNGNANNNGNGTGNGSYTNGNGNGNGKNKRNRVRRESSMRQQQVQLTFGSEADDPLIHEDFDFEGNLALFDKQAIWDDIESTTQKPDVVRHIVNNHHHKPEQKYRHDENILASKPLQLRQIESMFGGSQDFVTDDGLIIPTIPAYVRNKIEISADKAGLSLQRQIDILARGASDLAITLLGGARRLTPANNHQWPKIAIICDGGKNMRTINIGAATGRQLASHGLTVLLYVEQAKLLEQNSSSPEISLFKATDNVIVHSVDALPTPDLVILSTNTANLSDAIRKWLSVNRASVLAIDPPPCGINEVAIKYSILPILPLNGISTATTSSSSSAAATPTPIASTSAAASATKSAASTNNCGKLYLCNLGIPDKFYRDCGIKYKSPYGHKYVIPIHSKD

Target ORF information:

RefSeq Version	NM_140735.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of decapping 3 (Edc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140735.3

ORF Insert Sequence:

ATGGGTCCGA CGGATCAAGA CTGGATCGGC TGTGCAGTGT CTATAGCCTG CGACGAGGTC 
CTGGGCGTCT TCCAGGGCCT GATCAAGCAG ATTTCTGCCG AGGAGATCAC CATTGTGAGG 
GCTTTCCGCA ATGGCGTGCC GCTGCGAAAG CAGAACGCCG AGGTGGTCCT CAAGTGCACG 
GACATTCGCA GCATCGATCT CATCGAGCCC GCAAAGCAAG ATCTGGATGG GCACACGGCG 
CCGCCGCCAG TGGTCAACAA GCCCACGCCC GTCAAGCTGC CCCACTTCTC CAACATCCTG 
GGCAAGCAGC AGCAGCTCCA ACTCCAGCAG CAGCAGCAGC AGCTACAACT CCAGCAGCAA 
CAGAAACAGC AGTTCCAGGA GCAGGAACGA GAACAGGATC TGCCATCGAC GCCGCGGTCA 
AGGGCCAACG ACAATGGACG GGTAGCGGCT GGCGGAGCAT CGAGTTCTTC GGGCCCACGT 
GGCAACTTCA ATTCGGCGCT AAGCGATAAG ATGCATCAGC TGAAGCTAAT CGAGACCAAC 
GGCTCAAATG GCACCCTTAG AACACCACAA ACATCCCGTG CGTCCAGCGC TCAGCAGCAG 
CCGCAGATAT CAACGACTCC CAACAGCGTG GCCGCCTTTT TTGGCAACAT GATACCGCCG 
AAGGTCGAGG TGAAACTGGG CTCCTACGTG AGCAACACCC GGGAGAGTTA CTGCAGCAGC 
AGCGGAGATA GCGGCGAGGC AACTGGTTTG TCGTTGGGCA GCAGCAAACC CATAGATATC 
GTCAGCAACG GAGATGGATT TTACAAGCAG ACGGCAGCCT CGAGCTATGG GAATACAAAT 
GGAAATGTAA GAAGGAATGG CAATGCCAAT AACAATGGAA ATGGCACCGG AAATGGTTCT 
TATACCAATG GAAATGGCAA CGGCAACGGG AAGAACAAGC GGAACAGGGT GCGAAGGGAG 
AGCAGCATGC GGCAGCAACA GGTGCAACTG ACTTTTGGCA GCGAGGCGGA CGATCCGCTG 
ATCCACGAGG ATTTCGATTT TGAGGGCAAT CTAGCGCTGT TCGACAAGCA GGCAATTTGG 
GACGACATCG AGTCGACGAC CCAAAAACCA GATGTGGTGC GGCACATTGT GAACAATCAT 
CATCATAAGC CAGAGCAGAA GTATCGGCAC GACGAGAACA TCCTGGCCAG CAAACCACTA 
CAATTGCGCC AGATCGAAAG CATGTTCGGC GGCAGCCAGG ACTTTGTGAC GGACGACGGT 
CTCATTATAC CGACGATTCC GGCTTACGTT CGGAATAAAA TCGAGATAAG TGCCGATAAG 
GCGGGCCTCT CTCTGCAGAG GCAGATCGAC ATTTTGGCGC GCGGAGCCAG TGATCTGGCC 
ATTACACTGC TGGGCGGCGC CAGGCGATTG ACGCCGGCCA ACAACCATCA GTGGCCGAAG 
ATTGCCATCA TCTGCGATGG CGGCAAAAAT ATGAGAACAA TTAACATTGG AGCAGCCACA 
GGTCGCCAGC TGGCTTCGCA TGGGCTTACT GTCCTGCTGT ACGTGGAGCA GGCCAAACTG 
CTGGAGCAGA ACAGCAGCAG CCCAGAGATT TCATTGTTCA AGGCAACCGA CAATGTCATA 
GTTCACTCAG TAGACGCCTT ACCCACGCCA GATCTTGTGA TATTATCAAC TAACACCGCT 
AATTTATCGG ATGCGATCAG GAAATGGCTC AGTGTAAATC GCGCTTCGGT GCTTGCCATA 
GACCCGCCGC CGTGTGGCAT TAATGAGGTT GCTATCAAGT ACTCAATACT ACCCATACTA 
CCATTAAATG GCATATCAAC AGCAACAACA TCATCATCCT CTTCGGCTGC CGCCACACCA 
ACTCCCATTG CTAGCACTTC GGCAGCAGCA TCGGCTACCA AATCAGCAGC ATCAACGAAC 
AATTGTGGCA AATTGTATTT ATGTAATCTA GGTATACCGG ATAAATTCTA TCGTGATTGT 
GGCATTAAGT ACAAAAGTCC GTATGGACAT AAATATGTGA TACCGATTCA CTCAAAGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Edc3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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